Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| minimizing bias in virally seeded water treatment studies: evaluation of optimal bacteriophage and mammalian virus preparation methodologies. | one key assumption impacting data quality in viral inactivation studies is that reduction estimates are not altered by the virus seeding process. however, seeding viruses often involves the inadvertent addition of co-constituents such as cell culture components or additives used during preparation steps which can impact viral reduction estimates by inducing non-representative oxidant demand in disinfection studies and fouling in membrane assessments. the objective of this study was therefore to ... | 2017 | 28616834 |
| identification of amino acids within norovirus polymerase involved in rna binding and viral replication. | until recently, molecular studies on human norovirus (hunov), a major causative agent of gastroenteritis, have been hampered by the lack of an efficient cell culture system. murine norovirus-1 (mnv-1) has served as a surrogate model system for norovirus research, due to the availability of robust cell culture systems and reverse genetics. to identify amino acids involved in rna synthesis by the viral rna-dependent rna polymerase (ns7), we constructed ns7 mutants in which basic amino acids surrou ... | 2017 | 28640742 |
| viral population changes during murine norovirus propagation in raw 264.7 cells. | laboratory adaptation of viruses is an essential technique for basic virology research, including the generation of attenuated vaccine strains, although the principles of cell adaptation remain largely unknown. deep sequencing of murine norovirus (munov) s7 during serial passages in raw264.7 cells showed that the frequencies of viral variants were altered more dynamically than previously reported. serial passages of the virus following two different multiplicity of infections gave rise to distin ... | 2017 | 28663743 |
| inactivation of viruses and bacteria on strawberries using a levulinic acid plus sodium dodecyl sulfate based sanitizer, taking sensorial and chemical food safety aspects into account. | the efficacy of levulinic acid (lva) in combination with sodium dodecyl sulfate (sds) in removal of foodborne viruses, enteric bacterial pathogens and their surrogates on fresh strawberries was investigated. inoculated strawberries were treated with potable water, sodium hypochlorite solution (50ppm), 0.5% lva plus 0.5% sds solution, and 5% lva plus 2% sds solution respectively for 2min, followed by spray-rinsing with potable water. water washing removed at least 1.0-log of the tested viral and ... | 2017 | 28668727 |
| viral replication complexes are targeted by lc3-guided interferon-inducible gtpases. | all viruses with positive-sense rna genomes replicate on membranous structures in the cytoplasm called replication complexes (rcs). rcs provide an advantageous microenvironment for viral replication, but it is unknown how the host immune system counteracts these structures. here we show that interferon-gamma (ifng) disrupts the rc of murine norovirus (mnv) via evolutionarily conserved autophagy proteins and the induction of ifn-inducible gtpases, which are known to destroy the membrane of vacuol ... | 2017 | 28669671 |
| noroviruses co-opt the function of host proteins vapa and vapb for replication via a phenylalanine-phenylalanine-acidic-tract-motif mimic in nonstructural viral protein ns1/2. | the norovirus genus contains important human pathogens, but the role of host pathways in norovirus replication is largely unknown. murine noroviruses provide the opportunity to study norovirus replication in cell culture and in small animals. the human norovirus nonstructural protein ns1/2 interacts with the host protein vamp-associated protein a (vapa), but the significance of the ns1/2-vapa interaction is unexplored. here we report decreased murine norovirus replication in vapa- and vapb-defic ... | 2017 | 28698274 |
| inactivation of norovirus by lemongrass essential oil using a norovirus surrogate system. | this study investigated the effect of lemongrass essential oil (lgeo) on the infectivity and viral replication of norovirus. murine norovirus 1 (mnv-1), a surrogate of human norovirus, was preincubated with lgeo and then used to infect raw 264.7 cells in a plaque reduction assay. lgeo exhibited a significant reduction in mnv-1 plaque formation in both time- and dose-dependent manners. the quantification of viral genome by quantitative real-time pcr showed similar results in line with those of th ... | 2017 | 28699786 |
| new perspectives regarding the antiviral effect of vitamin a on norovirus using modulation of gut microbiota. | gut microbiota has been revealed to play an important role in various health conditions, and recent studies have suggested the modulation of gut microbiota as a therapeutic strategy. there is no effective treatment of norovirus infection, though vitamin a has been suggested to have an antiviral effect in an epidemiological study. we demonstrated that vitamin a significantly inhibited murine norovirus replication. vitamin a supplementation significantly increased the abundance of lactobacillus sp ... | 2017 | 28727498 |
| broad-spectrum non-nucleoside inhibitors for caliciviruses. | viruses of the caliciviridae cause significant and sometimes lethal diseases, however despite substantial research efforts, specific antivirals are lacking. broad-spectrum antivirals could combat multiple viral pathogens, offering a rapid solution when no therapies exist. the rna-dependent rna polymerase (rdrp) is an attractive antiviral target as it is essential for viral replication and lacks mammalian homologs. to focus the search for pan-caliciviridae antivirals, the rdrp was probed with non ... | 2017 | 28757394 |
| inhibition of calcineurin or imp dehydrogenase exerts moderate to potent antiviral activity against norovirus replication. | norovirus is a major cause of acute gastroenteritis worldwide and has emerged as an important issue of chronic infection in transplantation patients. since no approved antiviral is available, we evaluated the effects of different immunosuppressants and ribavirin on norovirus and explored their mechanisms of action by using a human norovirus (hunv) replicon-harboring model and a surrogate murine norovirus (mnv) infectious model. the roles of the corresponding drug targets were investigated by gai ... | 2017 | 28807916 |
| rna sequencing of murine norovirus-infected cells reveals transcriptional alteration of genes important to viral recognition and antigen presentation. | viruses inherently exploit normal cellular functions to promote replication and survival. one mechanism involves transcriptional control of the host, and knowledge of the genes modified and their molecular function can aid in understanding viral-host interactions. norovirus pathogenesis, despite the recent advances in cell cultivation, remains largely uncharacterized. several studies have utilized the related murine norovirus (mnv) to identify innate response, antigen presentation, and cellular ... | 2017 | 28848558 |
| recovery optimization and survival of the human norovirus surrogates feline calicivirus and murine norovirus on carpet. | carpets have been implicated in prolonged and reoccurring outbreaks of human noroviruses (hunov), the leading cause of acute gastroenteritis worldwide. viral recovery from environmental surfaces, such as carpet, remains undeveloped. our aim was to determine survival of hunov surrogates on an understudied environmental surface, carpet. first, we measured the zeta potential and absorption capacity of wool and nylon carpet fibers, we then developed a minispin column elution (msc) method, and lastly ... | 2017 | 28864657 |
| efficacy of ethanol against viruses in hand disinfection. | ethanol is used worldwide in healthcare facilities for hand rubbing. it has been reported to have a stronger and broader virucidal activity compared with propanols. the aim of this review was to describe the spectrum of virucidal activity of ethanol in solution or as commercially available products. a systematic search was conducted. studies were selected when they contained original data on reduction of viral infectivity from suspension tests (49 studies) and contaminated hands (17 studies). et ... | 2017 | 28882643 |
| inactivation of human norovirus genogroups i and ii and surrogates by free chlorine in postharvest leafy green wash water. | human noroviruses (hnovs) are a known public health concern associated with the consumption of leafy green vegetables. while a number of studies have investigated pathogen reduction on the surfaces of leafy greens during the postharvest washing process, there remains a paucity of data on the level of treatment needed to inactivate viruses in the wash water, which is critical for preventing cross-contamination. the objective of this study was to quantify the susceptibility of hnov genotype i (gi) ... | 2017 | 28887415 |
| application of gamma radiation for the reduction of norovirus and the quality stability in optimally ripened cabbage kimchi. | optimally ripened commercial cabbage kimchi is considered the main cause of enteric norovirus (nov) outbreaks in korea. this study investigated the effect of 1-10kgy gamma radiation on the inactivation of murine norovirus-1 (mnv-1; initial inoculum of 5-6log10pfu/ml), used as a human nov surrogate, in kimchi. the effects of gamma radiation on the ph and acidity were also examined to address the index of quality and fermentation, respectively. titers of mnv-1 significantly reduced (p<0.05) in kim ... | 2017 | 28888451 |
| reduction of human norovirus gi, gii, and surrogates by peracetic acid and monochloramine in municipal secondary wastewater effluent. | the objective of this study was to characterize human norovirus (hnov) gi and gii reductions during disinfection by peracetic acid (paa) and monochloramine in secondary wastewater (ww) and phosphate buffer (pb) as assessed by reverse transcription-qpcr (rt-qpcr). infectivity and rt-qpcr reductions are also presented for surrogate viruses murine norovirus (mnv) and bacteriophage ms2 under identical experimental conditions to aid in interpretation of hnov molecular data. in ww, rt-qpcr reductions ... | 2017 | 28910089 |
| evaluation of air samplers and filter materials for collection and recovery of airborne norovirus. | the aim of this study was to identify the most efficient sampling method for quantitative pcr-based detection of airborne human norovirus (nov). | 2017 | 28921812 |
| norovirus cell tropism is determined by combinatorial action of a viral non-structural protein and host cytokine. | cellular tropism during persistent viral infection is commonly conferred by the interaction of a viral surface protein with a host receptor complex. norovirus, the leading global cause of gastroenteritis, can be persistently shed during infection, but its in vivo cellular tropism and tropism determinants remain unidentified. using murine norovirus (mnov), we determine that a small number of intestinal epithelial cells (iecs) serve as the reservoir for fecal shedding and persistence. the viral no ... | 2017 | 28966054 |
| differentiation and protective capacity of virus-specific cd8+ t cells suggest murine norovirus persistence in an immune-privileged enteric niche. | noroviruses can establish chronic infections with active viral shedding in healthy humans but whether persistence is associated with adaptive immune dysfunction is unknown. we used genetically engineered strains of mouse norovirus (mnv) to investigate cd8+ t cell differentiation during chronic infection. we found that chronic infection drove mnv-specific tissue-resident memory (trm) cd8+ t cells to a differentiation state resembling inflationary effector responses against latent cytomegalovirus ... | 2017 | 29031786 |
| virucidal efficacy of food additive grade calcium hydroxide against surrogate of human norovirus. | an alkaline agent, namely, food additive grade calcium hydroxide (fdca(oh)2) in the solution, powder and suspension forms was evaluated as a virucidal agent, using a murine norovirus (mnv) as the surrogate for human norovirus. the main constituent of fdca(oh)2 is ca(oh)2, which has ph 13 in 0.17% solution. the results showed that 0.17% fdca(oh)2 solution could inactivate mnv within 30s even in the presence of organic materials (5% fetal bovine serum (fbs)). in a contaminated surface experiment, ... | 2018 | 29054741 |
| norovirus escape from broadly neutralizing antibodies is limited to allostery-like mechanisms. | ideal antiviral vaccines elicit antibodies (abs) with broad strain recognition that bind to regions that are difficult to mutate for escape. using 10 murine norovirus (mnv) strains and 5 human norovirus (hunov) virus-like particles (vlps), we identified monoclonal antibody (mab) 2d3, which broadly neutralized all mnv strains tested. importantly, escape mutants corresponding to this antibody were very slow to develop and were distal to those raised against our previously studied antibody, a6.2. t ... | 2017 | 29062895 |
| membrane alterations induced by nonstructural proteins of human norovirus. | human noroviruses (hunov) are the most frequent cause of non-bacterial acute gastroenteritis worldwide, particularly genogroup ii genotype 4 (gii.4) variants. the viral nonstructural (ns) proteins encoded by the orf1 polyprotein induce vesical clusters harboring the viral replication sites. little is known so far about the ultrastructure of these replication organelles or the contribution of individual ns proteins to their biogenesis. we compared the ultrastructural changes induced by expression ... | 2017 | 29077760 |
| interferon lambda (ifn-λ) efficiently blocks norovirus transmission in a mouse model. | human noroviruses are highly efficient in person to person transmission thus associated with explosive outbreaks of acute gastroenteritis. outbreak control is limited to disinfection and isolation measures. strategies to control the spread of noroviruses should be developed and models to study norovirus transmission will greatly facilitate this. here, a mouse-to-mouse transmission model, in which mice develop acute murine norovirus (mnv)-induced diarrhea, was used to explore the role of interfer ... | 2018 | 29080649 |
| virological quality of irrigation water sources and pepper mild mottle virus and tobacco mosaic virus as index of pathogenic virus contamination level. | irrigation water is a doorway for the pathogen contamination of fresh produce. we quantified pathogenic viruses [human adenoviruses, noroviruses of genogroups i and ii, group a rotaviruses, aichi virus 1 (aiv-1), enteroviruses (envs), and salivirus (saliv)] and examined potential index viruses [jc and bk polyomaviruses (jcpyvs and bkpyvs), pepper mild mottle virus (pmmov), and tobacco mosaic virus (tmv)] in irrigation water sources in the kathmandu valley, nepal. river, sewage, wastewater treatm ... | 2017 | 29098656 |
| the major targets of acute norovirus infection are immune cells in the gut-associated lymphoid tissue. | noroviruses are the leading cause of food-borne gastroenteritis outbreaks and childhood diarrhoea globally, estimated to be responsible for 200,000 deaths in children each year 1-4 . thus, reducing norovirus-associated disease is a critical priority. development of vaccines and therapeutics has been hindered by the limited understanding of basic norovirus pathogenesis and cell tropism. while macrophages, dendritic cells, b cells and stem-cell-derived enteroids can all support infection of certai ... | 2017 | 29109476 |
| the role of interferon in persistent viral infection: insights from murine norovirus. | persistent viral infections result from evasion or avoidance of sterilizing immunity, extend the timeframe of virus transmission, and can trigger disease. prior studies in mouse models of persistent infection have suggested that ineffective adaptive immune responses are necessary for persistent viral infection. however, recent work in the murine norovirus (mnv) model of persistent infection demonstrates that innate immunity can control both early and persistent viral replication independently of ... | 2017 | 29157967 |
| characterization of natural organic substances potentially hindering rt-pcr-based virus detection in large volumes of environmental water. | quantitative detection of pathogenic viruses in the environmental water is essential for the assessment of water safety. it is known that some of natural organic substances interfere with virus detection processes, i.e., nucleic acid extraction and reverse transcription-pcr. such substances are carried over into a sample after virus concentration. in this study, inhibitory substances in coastal water samples were characterized in view of their effects on efficiency of virus detection and propert ... | 2017 | 29165998 |
| inactivation of murine norovirus and hepatitis a virus on fresh raspberries by gaseous ozone treatment. | raspberries are vulnerable products for which industrial treatment solutions ensuring both food safety and sensory quality are not easily applicable. raspberries have been associated with numerous foodborne outbreaks in recent decades. ozone has been proven effective as a drinking water treatment against pathogenic microorganisms. nevertheless, to date, little information is available regarding the effect of gaseous ozone on viruses in food matrices. a comparison of the effect of gaseous ozone o ... | 2018 | 29173615 |
| virucidal efficacy of peracetic acid for instrument disinfection. | various peracetic-acid (paa)-based products for processing flexible endoscopes on the market are often based on a two-component system including a cleaning step before the addition of paa as disinfectant. the peracetic acid concentrations in these formulations from different manufacturers are ranging from 400 to 1500 ppm (part per million). these products are used at temperatures between 20 °c and 37 °c. since information on the virus-inactivating properties of peracetic acid at different concen ... | 2017 | 29177047 |
| evaluation of the suitability of a plant virus, pepper mild mottle virus, as a surrogate of human enteric viruses for assessment of the efficacy of coagulation-rapid sand filtration to remove those viruses. | here, we evaluated the removal of three representative human enteric viruses - adenovirus (adv) type 40, coxsackievirus (cv) b5, and hepatitis a virus (hav) ib - and one surrogate of human caliciviruses - murine norovirus (mnv) type 1 - by coagulation-rapid sand filtration, using water samples from eight water sources for drinking water treatment plants in japan. the removal ratios of a plant virus (pepper mild mottle virus; pmmov) and two bacteriophages (ms2 and φx174) were compared with the re ... | 2018 | 29182907 |
| heat-denatured lysozyme could be a novel disinfectant for reducing hepatitis a virus and murine norovirus on berry fruit. | hepatitis a virus (hav) is well known worldwide as a causative virus of acute hepatitis. in recent years, numerous cases of hav infection caused by hav-contaminated berries have occurred around the world. because berries are often consumed without prior heating, reliable disinfection of the raw fruit is important in order to prevent hav outbreaks. previous studies have found that murine norovirus strain 1 (mnv-1) and human norovirus gii.4 were inactivated in heat-denatured lysozyme solution. in ... | 2017 | 29202339 |
| testing for human norovirus and recovery of process control in outbreak-associated produce items. | the development of rapid and sensitive detection methods for human noroviruses (hunov) in produce items is critical, especially with the recent rise in outbreaks associated with this food commodity. in this study, 50-g portions of various produce items linked to a norovirus outbreak (celery, cucumber, lettuce, grapes, and radish) were artificially inoculated with murine norovirus (mnv-1) and concentrated either by ultracentrifugation or polyethylene glycol (peg) precipitation after elution with ... | 2018 | 29280676 |
| murine norovirus inhibits b cell development in the bone marrow of stat1-deficient mice. | noroviruses are a leading cause of gastroenteritis in humans and it was recently revealed that noroviruses can infect b cells. we demonstrate that murine norovirus (mnv) infection can significantly impair b cell development in the bone marrow in a signal transducer and activator of transcription 1 (stat1) dependent, but interferon signaling independent manner. we also show that mnv replication is more pronounced in the absence of stat1 in ex vivo cultured b cells. interestingly, using bone marro ... | 2017 | 29287229 |
| disinfection of waterborne viruses using silver nanoparticle-decorated silica hybrid composites in water environments. | silver nanoparticles (agnps) have been reported as an effective alternative for controlling a broad-spectrum of pathogenic viruses. we developed a micrometer-sized silica hybrid composite decorated with agnps (agnp-sio2) to prevent the inherent aggregation of agnps, and facilitated their recovery from environmental media after use. the production process had a high-yield, and fabrication was cost-effective. we evaluated the antiviral capabilities of ag30-sio2 particles against two model viruses, ... | 2017 | 29291562 |
| alcohols as surface disinfectants in healthcare settings. | isopropyl alcohol and ethyl alcohol have been used as low-level disinfectants in healthcare settings for many years. recent studies have found that ethyl alcohol inhibits protein synthesis in escherichia coli by direct effects on ribosomes and rna polymerase and that 60%-70% solutions have in vitro efficacy against murine norovirus, ebola virus, and several coronaviruses. alcohol prep pads or towelettes containing isopropyl or ethyl alcohol and water have been used primarily for disinfection of ... | 2018 | 29374503 |
| comparative transcriptomic response of primary and immortalized macrophages to murine norovirus infection. | murine norovirus (nov) is genetically similar to human nov and offers both an efficient in vitro cell culture system and an animal model by which to investigate the molecular basis of replication. in this study, we present a detailed global view of host alterations to cellular pathways that occur during the progression of a nov infection. this was accomplished for both mus musculus balb/c-derived raw264.7 (raw) cells, an immortalized cell line widely used in in vitro replication studies, and pri ... | 2018 | 29735480 |
| common vole (microtus arvalis) and bank vole (myodes glareolus) derived permanent cell lines differ in their susceptibility and replication kinetics of animal and zoonotic viruses. | pathogenesis and reservoir host adaptation of animal and zoonotic viruses are poorly understood due to missing adequate cell culture and animal models. the bank vole (myodes glareolus) and common vole (microtus arvalis) serve as hosts for a variety of zoonotic pathogens. for a better understanding of virus association to a putative animal host, we generated two novel cell lines from bank voles of different evolutionary lineages and two common vole cell lines and assayed their susceptibility, rep ... | 2019 | 31513859 |