Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| respiratory syncytial virus infection and prevalence of subgroups a and b in hawaii. | respiratory syncytial virus was isolated from hospitalized children in hawaii in each month of the year during the period january 1987 to august 1989. subgroup a and subgroup b strains cocirculated, with subgroup a predominating. there was an alternating early-season and late-season peak incidence cycle as reported elsewhere. | 1991 | 1774271 |
| rapid diagnosis of respiratory syncytial virus infection by using pernasal swabs. | the sensitivity and specificity of direct immunofluorescence microscopy performed on pernasal swab specimens and compared with those of nasopharyngeal aspirates were 93 and 99%, respectively. posterior nasopharyngeal swabs applied immediately to microscope slides allow a rapid and simple screening procedure for the diagnosis of acute respiratory syncytial virus infections. | 1991 | 1774282 |
| immune responses of lambs experimentally infected with bovine respiratory syncytial virus and pasteurella haemolytica. | the immune responses of lambs experimentally infected with bovine respiratory syncytial virus (rsv) and pasteurella haemolytica were compared with lambs infected with bovine rsv or pasteurella haemolytica alone. superinfection with p. haemolytica aggravated the reduction in the number of cd5+, cd4+ and lca p220+ (b) lymphocytes caused by bovine rsv, but it had no effect on the neutralizing antibodies against p. haemolytica cytotoxin. serum samples obtained from lambs experimentally infected with ... | 1991 | 1779037 |
| [medium-weight molecules and their indicator role in young children with acute pneumonia]. | as many as 63 infants with acute pneumonia, 7 children with arvi (bronchiolitis) and 28 normal children were examined. at the height of the clinical manifestations there was an increase of the level of medium-weight molecules in blood serum, correlating well with the disease gravity determined by the intensity of the toxic syndrome. the highest concentration of serum medium-weight molecules was demonstrable in toxic and purulent complications due to acute pneumonia. meanwhile the level of medium ... | 1991 | 1787993 |
| [the efficacy of ribamidil in experimental rs-virus infection]. | the antiviral activity of a national analogue of virasol, ribamydil, with regard to rs virus infection was studied in tissue culture and in experimental animals. in l-41 cell cultures ribamydil in a dose of 60 micrograms/ml or more completely inhibited multiplication of rs virus strain long, in a titre of 4.75 lg cpd50. the drug concentrations of 30, 15, and 7 micrograms/ml reduced the virus content by 3.75, 2.75 and 2.0 lg cpd50, respectively; ed50 of the drug was 7 micrograms/ml, the chemother ... | 1991 | 1796584 |
| vaccination against respiratory syncytial virus: problems and progress. | 1991 | 1797047 | |
| evaluation of a commercial enzyme immunomembrane filter assay for detection of respiratory syncytial virus in clinical specimens. | a commercial enzyme immunomembrane filter assay (eifa) for respiratory syncytial virus (rsv) was compared prospectively with isolation in cell culture and an enzyme immunoassay. a total of 595 respiratory specimens, mostly from pediatric patients, were examined. the eifa was 70.96% sensitive and 72.40% specific in comparison with cell culture. results for 40 specimens (6.72%) were uninterpretable, mainly due to filtration difficulties. twenty-one (25%) of 84 specimens whose results were initiall ... | 1991 | 1802701 |
| the seal death in danish waters 1988. 2. virological studies. | mass abortions and high mortality were observed in harbour seals in danish waters during 1988. severe pneumonia and emphysema were typical clinical and post-mortem findings. virological studies were carried out to identify the cause of the epidemic. although seal herpesvirus (sehv) was isolated in 23 of 114 animals this virus was subsequently found not to be the primary cause of the disease. following the observation of seroconversion against canine distemper virus (cdv) in diseased seals (oster ... | 1991 | 1803934 |
| comparative study of respiratory syncytial virus in nasopharyngeal aspirates using conventional cell culture, shell viral centrifugation culture, immunofluorescence and biotin-avidin enzyme linked immunosorbent assays. | 133 nasopharyngeal aspirates (npa) were simultaneously tested for the presence of respiratory syncytial virus (rsv) by conventional cell culture (ccc), shell vial centrifugation culture (svc), immunofluorescence assay (ifa) and biotin-avidin enzyme linked immunosorbent assay (b-a elisa). these yielded positive results in 32(24%), 45(33.8%), 36(27%) and 40(30%) of specimens, respectively. specimens positive by ifa and b-a elisa were all also positive by svc. the sensitivity of ccc, ifa, and b-a e ... | 1991 | 1807259 |
| [identification of rs virus by using reverse transcriptase polymerase chain reaction]. | 1991 | 1810089 | |
| clinical characteristics of respiratory syncytial virus (rsv) subgroup infections in japan. | the subgroup characteristics of 130 strains of respiratory syncytial virus (rsv) isolated in sapporo during 9 epidemic years 1980-1989 were determined. monoclonal antibodies raised against the rsv long strains were used. subgroup a included 77 (59.2%) isolates and subgroup b 52 (40.0%) strains, while 1 strain was considered to be a variant of a subgroup a strain. the distribution by age of infants and children was different for the 2 subgroups: less than 1 year of age infants with subgroup a inf ... | 1991 | 1815326 |
| pulmonary epithelial t cells induced by viral infection express t cell receptors alpha/beta. | cells were recovered from the lungs of mice by bronchoalveolar lavage before and after infection with respiratory syncytial (rs) virus. uninfected mice yielded mostly macrophages, but after rs virus infection lymphocytes also appeared. recovered cells were stained for cd4, cd8' and either cd3, t cell receptor (tcr) alpha/beta or tcr gamma/delta and analyzed by 3-color flow cytometry. both the cd4+cd8- and cd4-cd8+ subpopulations stained uniformly for cd3 and tcr alpha/beta, while none stained fo ... | 1991 | 1826265 |
| [isolation of a paramyxovirus-3 from turkeys with respiratory tract disease in germany]. | in fattening turkeys 2.5 weeks of age a respiratory disease associated with coughing, nasal discharge and swelling of the infraorbital sinus was seen. pathological findings in diseased turkeys were sinusitis, tracheitis, pneumonia and aerosacculitis. virological investigations of trachea, kidney and intestine in spf-chicken embryos resulted in the isolation of a virus, that could be identified as a paramyxovirus type 3 due to chemical-physical, biological, morphological and immunological propert ... | 1991 | 1829671 |
| immunological relationships between parainfluenza virus type 4 and other paramyxoviruses studied by use of monoclonal antibodies. | these results demonstrated that cross-reactions between mumps virus, piv-2, piv-4, and sv5 involve both internal components and surface glycoproteins and that these viruses form a separate group within the paramyxovirus genus. | 1991 | 1848067 |
| etiology of acute lower respiratory tract infections in gambian children: i. acute lower respiratory tract infections in infants presenting at the hospital. | ninety infants less than 1 year of age with pneumonia and 43 control infants were investigated for viral and chlamydial infection with the use of culture and serology and for bacterial infection with the use of blood cultures, lung aspirates, antibody assays and antigen detection procedures. one or more potential pathogens were identified in 62 (69%) cases with pneumonia and in 12 (28%) controls. infection by respiratory viruses was identified in 42 (49%) cases and in 8 (19%) controls. respirato ... | 1991 | 1848364 |
| numerous transitions in human parainfluenza virus 3 rna recovered from persistently infected cells. | the nucleotide sequences at the 3'-termini of human parainfluenza virus 3 (hpiv3) genomic rnas recovered from two lines of persistently infected llc-mk2 cells were determined following pcr amplification. after 29 months of persistence the 3'-end of the hpiv3 genome was found to be highly mutated. interestingly, the only types of nucleotide changes observed were u to c and a to g transitions. both u to c and a to g transitions were present on individual rna molecules. the data indicate that biase ... | 1991 | 1849685 |
| rescue of a sendai virus di genome by other parainfluenza viruses: implications for genome replication. | using a defective interfering sendai virus stock (dih4) freed of nondefective helper virus, we found that the closely related parainfluenza viruses 1 and 3 could substitute for the sendai virus helper in replicating dih4, creating chimeric nucleocapsids. the morbillivirus measles and the rhabdovirus vsv could not substitute. when dih4 is incubated intracellularly for 5 days in the absence of help, the ability of piv3 to rescue dih4 at this time depended on fresh sendai virus polymerase. the piv3 ... | 1991 | 1850900 |
| cardiovascular effects of acute bronchiolitis. | twenty-one children with normal hearts were studied during acute bronchiolitis. doppler echocardiography showed tricuspid valve regurgitation in 11 patients, many of whom had evidence of raised pulmonary artery systolic pressure. serial studies in those with severe infection showed that tricuspid regurgitation disappears with clinical improvement. | 1991 | 1851367 |
| the causes of hospital-treated acute lower respiratory tract infection in children. | to determine the etiologic agents in children with acute lower respiratory infection. | 1991 | 1852095 |
| acute and chronic giant cell hepatitis: a paramyxovirus infection? | 1991 | 1860651 | |
| etiology of acute respiratory infections in children in tropical southern india. | nasopharyngeal secretions and throat-swab specimens from 809 children less than 6 years old with acute respiratory infection were examined by culture and indirect immunofluorescence for the presence of virus or viral antigen. blood was cultured for the presence of bacteria in selected cases of lower respiratory infection (lri); pleural fluid also was cultured in cases of empyema. viruses were detected in 163 (49%) of 331 children with lri. respiratory syncytial virus (rsv) was the commonest agen ... | 1991 | 1862277 |
| reducing hospital respiratory infections. | 1991 | 1866260 | |
| [nosocomial virus infections in pediatric departments. rotavirus and respiratory syncytial virus]. | rotavirus and respiratory syncytial virus are common causes of acute gastroenteritis and upper and lower respiratory diseases among infants and toddlers. both viruses occur as epidemics during the winter season. the symptoms may be quite severe and often lead to hospitalisation in paediatric wards i.e. about 2,000 children per year. the viruses frequently give rise to nosocomial infections in the infant/toddler wards about one third of the cases being nosocomial. recent studies have revealed tha ... | 1991 | 1866810 |
| antibody enhancement of respiratory syncytial virus stimulation of leukotriene production by a macrophagelike cell line. | the clinical and epidemiologic features of respiratory syncytial virus (rsv) infections suggest that rsv-specific antibody may sometimes contribute to the disease process. recently, it has been demonstrated that virus-specific antibody can enhance rsv infection of macrophagelike cells in vitro. we evaluated the possibility that antibody might also enhance rsv stimulation of the bronchoactive mediator of inflammation leukotriene c-4 (ltc4)in a macrophagelike cell line, u937. the addition of rsv l ... | 1991 | 1870211 |
| [dynamics of local defensive reactions in the lungs in acute experimental inflammatory process induced by respiratory syncytial virus]. | study has been made of broncho-associated lymphoid tissue (balt), the number and functional activity of lung phagocytes, with 42 mice balb/c, induced by respiratory-syncytial virus. with virus inflammation a decreased number of lung macrophages, their stronger attraction and increased lung chemotaxis have been observed. the addition of bacterial infection was accompanied by an increase in lung neutrophil, lymphocyte and macrophage, a decrease in lung neutrophil chemotaxis, an increase in macroph ... | 1991 | 1878569 |
| ribavirin therapy for acute bronchiolitis: need for appropriate controls. | 1991 | 1880677 | |
| epidemiology of respiratory syncytial virus infection among infants over three winter seasons. | a study involving respiratory syncytial virus was carried out on infants and young children hospitalised with acute respiratory tract infection over the period december 1987 to march 1990. during peak periods of rs virus activity 420 naso-pharyngeal aspirates were submitted to the virus reference laboratory, department of medical microbiology, u.c.d., mostly from the dublin region. using immunofluorescence, enzyme linked immunosorbent assay and cell culture techniques 206 samples (49%) were iden ... | 1991 | 1885284 |
| reinfection of mice with respiratory syncytial virus. | a balb/c model of respiratory syncytial virus infection has been developed in which high-titered replication occurs in lung, immunological infiltrates in lung can be detected histologically, and illness can be consistently reproduced. the immunodeterminants of rsv reinfection in this system were investigated by rechallenging mice with rsv early (less than 2 months after primary infection) and late (16 to 21 months after primary infection) and correlating illness and titer of rsv isolated from lu ... | 1991 | 1885946 |
| experimental reproduction of respiratory tract disease with bovine respiratory syncytial virus. | an experiment was conducted to reproduce respiratory tract disease with bovine respiratory syncytial virus (brsv) in one-month-old, colostrum-fed calves. the hypothesized role of viral hypersensitivity and persistent infection in the pathogenesis of brsv pneumonia was also investigated. for brsv inoculation a field isolate of brsv, at the fifth passage level in cell culture, was administered by a combined respiratory tract route (intranasal and intratracheal) for four consecutive days. four grou ... | 1991 | 1887565 |
| reliability of two new test kits for rapid diagnosis of respiratory syncytial virus infection. | two new rapid enzyme immunoassays (eias) for detecting respiratory syncytial virus (rsv), directigen (becton dickinson microbiology systems) and testpack (abbott diagnostics) were compared with virus isolation and direct immunofluorescence by using fresh specimens. the sensitivities of both eias were low (72 to 73%), but when initial specimens were used, testpack had a high sensitivity (92%) in contrast to that of directigen (76%). because of its high sensitivity and specificity, testpack can be ... | 1991 | 1890186 |
| community- and hospital-acquired respiratory syncytial virus infections in chile. | nasopharyngeal aspirates were obtained on admission from 614 patients younger than 2 years of age who were hospitalized in a ward for acute respiratory infections from june 1988 through october, 1989, in santiago, chile. patients in two rooms were followed during the cold seasons by sampling aspirates every other day during the child's entire hospital stay. clinical features were recorded daily. indirect monoclonal immunofluorescent assay and isolation in hep-2 were used for respiratory syncytia ... | 1991 | 1891287 |
| solid matrix-antibody-antigen complexes induce antigen-specific cd8+ cells that clear a persistent paramyxovirus infection. | we have previously shown that the adoptive transfer of splenocytes, isolated from mice immunized by infection with the paramyxovirus simian virus 5 (sv5), enhance the speed of clearance of sv5 from the lungs of immunodeficient mice; clearance is mediated primarily through cd8+ effector cells and not by serum neutralizing antibody (d.f. young, r.e. randall, j.a. hoyle, and b.e. souberbielle, j. virol. 64:5403-5411, 1990). in this article we demonstrate that immunization of mice with solid matrix- ... | 1991 | 1898971 |
| a controlled trial of aerosolized ribavirin in infants receiving mechanical ventilation for severe respiratory syncytial virus infection. | although the antiviral agent ribavirin improves the course of lower respiratory tract disease in spontaneously breathing infants with respiratory syncytial virus infection, it is not known whether ribavirin can benefit infants with severe respiratory syncytial virus disease who require mechanical ventilation. | 1991 | 1904551 |
| effect of respiratory syncytial virus infection on mice with protein malnutrition. | respiratory syncytial virus (rsv) pulmonary infection was produced in balb/c mice fed protein-deficient diets in an effort to understand the severity of viral pneumonia in infants in developing countries. as in previously published experiments with sendai virus, animals on the deficient diet became clinically malnourished, and certain aspects of their cell-mediated immunity were altered. the course of rsv infection in protein-deprived mice was essentially identical to that in normally nourished ... | 1991 | 1906929 |
| respiratory syncytial virus infection in anti-mu-treated mice. | balb/c mice were depleted of b cells by anti-mu treatment to investigate the pathogenesis of respiratory syncytial virus (rsv) infection in the absence of antibody. termination of rsv replication after primary infection occurred with the same kinetics in anti-mu-treated mice as in phosphate-buffered saline (pbs)-treated controls. yet, when rechallenged, anti-mu-treated mice were more permissive to rsv replication than pbs-treated controls. anti-mu-treated mice also experienced greater illness th ... | 1991 | 1908028 |
| role of t lymphocyte subsets in the pathogenesis of primary infection and rechallenge with respiratory syncytial virus in mice. | the role of cd4+ and cd8+ t lymphocytes in terminating respiratory syncytial virus (rsv) replication, causing disease, and protecting from reinfection was investigated using a balb/c mouse model in which cd4+ or cd8+ lymphocytes or both were depleted by injections of mab directed against the respective mouse lymphocyte determinants. kinetics of rsv replication, illness, and pathology were assessed after primary infection and rechallenge. both cd4+ and cd8+ lymphocyte subsets were involved in ter ... | 1991 | 1909350 |
| respiratory syncytial virus and supraventricular tachycardia in an infant. | a 7-week infant admitted with bronchiolitis proven to be caused by respiratory syncytial virus developed a supraventricular tachycardia which responded to digitalization. she has remained well and no longer requires medication 6 months later, her electrocardiogram now being normal. the course of the illness suggests an association between infection with respiratory syncytial virus and the development of the tachycardia. | 1991 | 1917175 |
| clinical observations on lower respiratory tract infections with special reference to serum ige levels. | to examine if the occurrence of wheezing among children with lower respiratory tract infections is associated with atopic predisposition, serum levels of ige were determined for groups of children with or without wheezing, during infection with mycoplasma pneumoniae or respiratory syncytial virus (rsv). in m. pneumoniae infection, more than 60% of wheezy children over 2 years of age showed a high ige level (200 iu/ml or more), while ige levels were less than 200 iu/ml in 73% of non-wheezy childr ... | 1991 | 1923666 |
| subacute effects of respiratory syncytial virus infection on lung function in lambs. | we examined the effects of ovine respiratory syncytial virus (rsv) infection on lung mechanics, lung histology, and airway reactivity in lambs. nine lambs were inoculated with ovine rsv and seven control lambs with normal saline or viral media. serum neutralization titers were obtained prior to and 3 weeks post-inoculation (pi). open lung biopsies were performed 1 and 3 weeks pi. lung mechanics including dynamic compliance (cdyn), resistance of the lung (rl), and functional residual capacity (fr ... | 1991 | 1923668 |
| respiratory syncytial virus infection among intubated adults in a university medical intensive care unit. | respiratory syncytial virus is the major cause of lower respiratory tract infection in children. adults who are immunocompromised, aged, institutionalized, and/or have underlying medical diseases may be at risk for severe rsv infection. intubated adults in an micu were evaluated for evidence of rsv infection. respiratory secretions were analyzed by cell culture and rsv eia. serologic testing was obtained. respiratory secretions from micu personnel with acute respiratory symptoms and patients adm ... | 1991 | 1935297 |
| value of bronchoalveolar lavage in diagnosing severe respiratory syncytial virus infections in infants. | 1991 | 1941381 | |
| ultrastructural features of alveolar lesions in induced respiratory syncytial virus pneumonia of calves. | ultrastructural changes occurred in alveolar epithelium in the acute and repair stages of induced respiratory syncytial virus pneumonia induced in eight calves (calf nos. 1-7, 3 to 6 days old and calf no. 8, 2 weeks old), using a bovine strain of respiratory syncytial virus. five of the calves were friesians, three were hereford x friesians, and all were male. tissues from three mock-infected control calves (two friesian, one hereford x friesian) were also examined. evidence of respiratory syncy ... | 1991 | 1949507 |
| ultrastructural features of lesions in bronchiolar epithelium in induced respiratory syncytial virus pneumonia of calves. | ultrastructural changes were observed in bronchioles in acute and repair stages of respiratory syncytial virus pneumonia induced in eight young calves (calf nos. 1-8) using a bovine strain of respiratory syncytial virus. five of the calves were friesians and three were hereford x friesians and all were male. tissues from three mock-infected control calves (two friesian, one hereford x friesian) were also examined. calves were from 3 to 6 days old at the time of first inoculation, with the except ... | 1991 | 1949508 |
| cytotoxic t cell responses in lambs experimentally infected with bovine respiratory syncytial virus. | the role of cell-mediated immune response in the immunopathogenesis of bovine respiratory syncytial virus (brsv) infection is not well established. in the present study, cytotoxic t cell responses of brsv-infected lambs were examined using the chromium release assay. lambs experimentally infected with brsv developed cytotoxic lymphocytes in the peripheral blood and the spleen, which lysed brsv-infected but not uninfected cells. peak cytotoxic activity occurred 10-14 days after infection. pretrea ... | 1991 | 1949569 |
| effects of bovine respiratory syncytial virus on airway function in neonatal calves. | respiratory syncytial virus (rsv) infection causes severe lower respiratory tract disease in infants and calves. neonatal respiratory tract infection in children often produces persistent changes in lung function. the specific objective of this study was to determine whether neonatal calves have transient or persistent alterations in pulmonary function and airway reactivity following rsv infection. six 2- to 3-day-old holstein bull calves were inoculated with 10 ml of bovine respiratory syncytia ... | 1991 | 1952323 |
| ribavirin therapy of adult respiratory syncytial virus pneumonitis. | respiratory syncytial virus is a common respiratory tract pathogen in infants. pulmonary infection in adult and elderly populations can occur with severe and even fatal pneumonitis having been reported in several recent outbreaks. we present a previously healthy adult patient who developed respiratory syncytial virus pneumonia severe enough to require mechanical ventilation. antiviral therapy with aerosolized ribavirin was successfully undertaken and the patient recovered completely. respiratory ... | 1991 | 1953237 |
| ribavirin for severe rsv infection. | 1991 | 1961230 | |
| viruses associated with acute lower respiratory tract infections in children from the eastern highlands of papua new guinea (1983-1985). | this study, conducted at goroka hospital from january 1983 to june 1985, examined the viruses identified in nasopharyngeal aspirates (npa) and urines collected from 716 hospitalised children with moderate or severe pneumonia, in npa from 170 children with mild pneumonia treated as outpatients and in npa from a control group of 428 children attending the outpatient department of goroka hospital suffering from minor ailments other than upper or lower respiratory tract infections. one or more virus ... | 1990 | 1963705 |
| an igm specific elisa for the serodiagnosis of viral bovine respiratory infections. | a capture elisa for the detection of igm antibodies to infectious bovine rhinotracheitis (ibr) and to bovine respiratory syncytial (brs) viruses was developed. in these assays, the first monoclonal antibody to bovine igm is used as the catching antibody while the second monoclonal detects specific antiviral antibodies. the test was evaluated on serum samples originating from both experimentally and naturally infected animals. from these studies, it has been shown that primary ibr and brs virus i ... | 1990 | 1963838 |
| [clinical observations in acute viral respiratory tract infections in infants under 2 years of age]. | 1990 | 1964742 | |
| [hospital acute respiratory infections]. | to study the incidence of nosocomial respiratory tract infection along the first week after admission, 31 children under 2 years of age admitted to the pediatric wards of a general hospital at metropolitan santiago, chile, with the diagnosis of lower respiratory tract infection, were studied for viral agents by immunofluorescence tests, viral isolation from pharyngeal aspirates, paired serum viral antibodies and bacterial cultures, all of them performed at admission and 4 to 5 days later from ma ... | 1990 | 1965058 |
| evaluation of the toxicity and antiviral activity of carbocyclic 3-deazaadenosine against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. | the toxicity and antiviral efficacy of carbocyclic 3-deazaadenosine (cc3ado) against respiratory syncytial (rsv) and parainfluenza type 3 (piv3) virus infections were tested in tissue culture and in cotton rats. the mean median efficacious dose (ed50) of cc3ado in hep2 cells against rsv and piv3 was 9 and 14 micrograms/ml, respectively. these values were 85- and 55-fold less than the median inhibitory (toxic) dose (id50) of cc3ado in this cell line (750 micrograms/ml), and similar to values obta ... | 1990 | 1965109 |
| toxicity and antiviral activity of ly253963 against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. | ly253963, the sodium salt of 1,3,4-thiadiazol-2-ylcyanamide, was evaluated in tissue culture and in cotton rats for toxicity and antiviral efficacy against respiratory syncytial (rsv) and parainfluenza type 3 (piv3) viruses. the selective index (ratio of the median toxic dose: median efficacious dose) of ly253963 in hep2 tissue culture cells was greater than 100 against both rsv and piv3. when given intraperitoneally to cotton rats, the minimum protective dose of ly253963 against both of these v ... | 1990 | 1965110 |
| host cell-dependent lateral mobility of viral glycoproteins. | the lateral mobility of viral envelope proteins on the plasma membranes of infected cells is an important factor in both virus assembly and pathogenesis. the envelope glycoproteins of measles and human parainfluenza virus are mobile on the surfaces of infected hela cells and undergo lateral redistribution in the presence of specific antibody, forming unipolar caps. in contrast, no such redistribution was observed with influenza virus hemagglutinin (ha) or vesicular stomatitis virus (vsv) g glyco ... | 1990 | 1965847 |
| [respiratory viral infections in young children 1988-1990]. | from october 1988 to june 1989 the studies on viral infections of respiratory tract were done in specimens taken from 461 children, aged 0-2 years. similarly to the observations in previous epidemic seasons infections due to rs and parainfluenza type 3 viruses were dominated. these studies included also influenza type c and parainfluenza type 4 viruses with unknown epidemiology in poland. the insignificant role of these infections in small children was estimated according to the low ratio of det ... | 1990 | 1966118 |
| parvovirus, rsv and cmv infections. | 1990 | 1966688 | |
| [acute lower respiratory viral infections]. | 1990 | 1967049 | |
| antiviral effect of 6-diazo-5-oxo-l-norleucine, antagonist of gamma-glutamyl transpeptidase, on replication of human parainfluenza virus type 2. | the antiviral effects of 6-diazo-5-oxo-l-norleucine (l-don) on the replication of human parainfluenza virus type 2 (hpiv-2), mumps and vesicular stomatitis viruses were studied. l-don suppressed growth of these viruses and, in particular, hpiv-2 in four cell types. l-don was not toxic to the cells at the active dose and did not significantly inhibit cellular macromolecular synthesis. the l-don-sensitive step of hpiv-2 replication was considered to be relatively early. the np, p and m proteins we ... | 1990 | 1968085 |
| respiratory syncytial virus infection in special care nursery. | 1990 | 1970055 | |
| causes of excess mortality in the netherlands and england and wales 1989/90. | 1990 | 1976180 | |
| clearance of a persistent paramyxovirus infection is mediated by cellular immune responses but not by serum-neutralizing antibody. | infection of the lungs of immunodeficient mice with the paramyxovirus simian virus 5 (sv5) was prolonged compared with the time course of infection in immunocompetent mice. although there was a significant increase in both viral rna and proteins, little infectious virus was produced. adoptive transfer of immune lymphocytes (isolated from the spleens of mice previously infected with sv5) but not of nonimmune lymphocytes increased the speed of clearance of virus from the lungs of immunodeficient m ... | 1990 | 1976819 |
| ribavirin therapy for respiratory syncytial virus infections. a scientific workshop. long beach, california, september 26-27, 1989. proceedings. | 1990 | 1978280 | |
| effectiveness of enteric immunization in the development of secretory immunoglobulin a response and the outcome of infection with respiratory syncytial virus. | cotton rats were immunized via intranasal, intradermal, or enteric routes with respiratory syncytial virus (rsv) or a live recombinant vaccinia virus expressing the rsv f glycoprotein (vaccinia f). the animals were tested for the appearance of rsv-specific antibody responses in the serum, bronchoalveolar lavage, and nasal wash after immunization and for virus replication 4 days after intranasal challenge with rsv. rsv antibody response in the serum and respiratory tract was demonstrated in all i ... | 1991 | 1987374 |
| ribavirin safety alert. | 1991 | 1988073 | |
| syncytial giant-cell hepatitis. sporadic hepatitis with distinctive pathological features, a severe clinical course, and paramyxoviral features. | we describe a new form of hepatitis, occurring in 10 patients over a period of six years, characterized clinically by manifestations of severe hepatitis, histologically by large syncytial giant hepatocytes, and ultrastructurally by intracytoplasmic structures consistent with paramyxoviral nucleocapsids. | 1991 | 1988831 |
| nosocomial transmission of respiratory syncytial virus in immunocompromised adults. | respiratory syncytial virus (rsv) isolates obtained from nine infected immunocompromised adult patients hospitalized during two consecutive winters (january through april 1987 and 1988) were collected and analyzed against a panel of monoclonal antibodies by an enzyme immunoassay. the history of the patients' illness, onset of symptoms, and date of initial isolation of virus was correlated with the hospital ward and time of hospitalization. three patients died of respiratory failure related to rs ... | 1991 | 1993745 |
| a single-season epidemic with respiratory syncytial virus subgroup b2 during 10 epidemic years, 1978 to 1988. | respiratory syncytial virus strains isolated from infants and children hospitalized with acute respiratory tract disease in huntington, w. va., from july 1979 through june 1988 were categorized by subgroup by using two panels of monoclonal antibodies specific for subgroup a and subgroup b. of 405 strains tested, 319 (78.8%) were subgroup a, 73 (18.0%) were subgroup b1, and 13 (3.2%) were subgroup b2. the subgroup b2 strains occurred as a single-season epidemic during the epidemiologic year july ... | 1991 | 1993752 |
| respiratory syncytial virus heterogeneity during an epidemic: analysis by limited nucleotide sequencing (sh gene) and restriction mapping (n gene). | the genes encoding the small hydrophobic (sh) proteins of a series of respiratory syncytial (rs) virus strains were amplified using the polymerase chain reaction, cloned and sequenced. analysis of the sh gene sequences from 12 rs virus strains isolated between 1956 and 1989 confirmed the homogeneity of the two subgroups. a and b, previously defined serologically. although there is only 76% deduced amino acid sequence identity of sh proteins between subgroups, there was little variation in deduce ... | 1991 | 1993875 |
| pattern of respiratory syncytial virus epidemics in finland: two-year cycles with alternating prevalence of groups a and b. | time-resolved fluoroimmunoassay with monoclonal antibodies distinguishing between respiratory syncytial virus (rsv) group a and b strains was used to analyze their prevalence in finland during 1981-1990 among 3285 patients with laboratory diagnosis of rsv, most of them hospitalized. the group typing of antigens in 608 rsv-positive nasopharyngeal aspirates showed a regular alternation of group prevalence, following the cyclic occurrence of the virus. group a predominated in 73%-90% of specimens f ... | 1991 | 1995719 |
| vaccination of cotton rats with a chimeric fg glycoprotein of human respiratory syncytial virus induces minimal pulmonary pathology on challenge. | the cotton rat model of human respiratory syncytial virus (rsv) infection was used to study the safety and efficacy of a chimeric fg glycoprotein that was expressed in insect cells using a baculovirus vector. histologic and virologic examination of vaccinated rat lungs was done after challenge with rsv. when rats were challenged 1 month after vaccination, severe pulmonary inflammation characterized by both a mononuclear and polymorphonuclear cell infiltrate and 30%-40% involvement of lung tissue ... | 1991 | 1995720 |
| age-related prevalence of serum antibody to respiratory syncytial virus in ecuadorian and german children. | 1991 | 1995747 | |
| respiratory syncytial virus (rsv) f, g, m2 (22k), and n proteins each induce resistance to rsv challenge, but resistance induced by m2 and n proteins is relatively short-lived. | the ability of recombinant vaccinia viruses that separately encoded 9 of the 10 known respiratory syncytial virus (rsv) proteins to induce resistance to rsv challenge was studied in balb/c mice. resistance was examined at two intervals following vaccination to examine early (day 9) as well as late (day 28) immunity. balb/c mice were inoculated simultaneously by the intranasal and intraperitoneal routes with a recombinant vaccinia virus encoding one of the following rsv proteins: f, g, n, p, sh, ... | 1991 | 1995956 |
| respiratory syncytial virus infections at rhode island hospital: use of ribavirin. | 1991 | 2000472 | |
| handwashing and cohorting in prevention of hospital acquired infections with respiratory syncytial virus. | hospital acquired infections with respiratory syncytial virus are a major problem. the virus is spread predominantly by infected nasal secretions and we investigated whether we could reduce its incidence by cohorting babies on each ward into designated areas and encouraging staff and parents to wash their hands. we examined the incidence of hospital acquired infection due to respiratory syncytial virus in all children less than 2 years old and in those with congenital heart disease. in 1986-7, b ... | 1991 | 2001109 |
| etiology of acute lower respiratory tract infections in gambian children: ii. acute lower respiratory tract infection in children ages one to nine years presenting at the hospital. | seventy-four children ages 1 to 9 years hospitalized because of severe pneumonia were investigated using blood cultures, lung aspirates, nasopharyngeal aspirates, serology and antigen detection procedures. a bacterial infection was identified in 57 (77%), a viral infection was seen in 25 (34%) and 18 (24%) had mixed viral-bacterial infections. the bacterial pathogens most frequently identified were streptococcus pneumoniae and haemophilus influenzae found in 61 and 15% of patients, respectively. ... | 1991 | 2003054 |
| respiratory syncytial virus infection of human mononuclear phagocytes stimulates synthesis of platelet-activating factor. | production of platelet-activating factor 1-o-alkyl-2-acetyl-sn-glycero-3- phosphocholine (paf), a potent mediator of inflammation, by mononuclear phagocytes varies with their stage of cellular differentiation and the nature of the eliciting stimulus. the human monocytic cell line u937 can be induced to differentiate to a macrophage-like cell following phorbol myristate acetate exposure, and after differentiation, these cells efficiently support replication of respiratory syncytial virus (rsv). u ... | 1991 | 2005091 |
| respiratory syncytial virus infection in infants with unequal pulmonary perfusion. | 1991 | 2005950 | |
| evaluation of immunofluorescence, two enzyme immunoassays and the shell-vial assay for detection of respiratory syncytial virus. | 1991 | 2009879 | |
| multicenter study of strains of respiratory syncytial virus. | two major groups of respiratory syncytial virus (rsv) strains, a and b, have been identified and their patterns of isolation determined in different communities but not simultaneously in multiple communities. in this study, we tested 483 rsv isolates from 14 university laboratories in the united states and canada for the 1984/1985 and 1985/1986 rsv seasons; 303 (63%) isolates were group a, 114 (24%) were group b, and 66 (14%) could not be grouped. isolates were subdivided into six subgroups with ... | 1991 | 2010623 |
| immunity to and frequency of reinfection with respiratory syncytial virus. | to better understand the duration of immunity against respiratory syncytial virus (rsv) and the role of serum antibodies to the surface glycoproteins, f and g, in susceptibility to reinfection, 15 adults with previous natural rsv infection were challenged with rsv of the same strain group (a) at 2, 4, 8, 14, 20, and 26 months after natural infection. by 2 months about one-half and by 8 months two-thirds of the subjects became reinfected. each challenge resulted in infection in at least one-fourt ... | 1991 | 2010624 |
| antigenic and genomic diversity within group a respiratory syncytial virus. | antigenic analysis using monoclonal antibodies and genomic analysis using ribonuclease protection was done on 47 isolates of group a respiratory syncytial virus (rsv) recovered from children in st. louis during four rsv seasons. antigenic analysis identified four subgroups; of the three that included more than one member, those designated a/2 and a/2v had characteristic ribonuclease protection patterns. a third subgroup, a/4, exhibited more extensive genomic heterogeneity, but all isolates were ... | 1991 | 2010638 |
| respiratory syncytial virus infections on an adult medical ward. | eleven cases of respiratory syncytial virus (rsv) infection occurred in acutely ill hospitalized adults over a 7-week period. nosocomial illness was suspected in two patients. because rsv can cause serious infections in immunocompromised adults with the potential for nosocomial spread, the following recommendations are indicated: (1) during the winter months, early recognition and diagnosis of rsv infections both in hospital staff and in patients should be encouraged; (2) infected hospital perso ... | 1991 | 2012452 |
| clinical and epidemiologic aspects of respiratory syncytial virus antigenic variants in argentinian children. | 1991 | 2019767 | |
| randomized trial of salbutamol in acute bronchiolitis. | to test whether nebulized salbutamol (albuterol) is safe and efficacious for the treatment of young children with acute bronchiolitis, we enrolled 83 children (median age 6 months, range 1 to 21 months) in a randomized, double-blind clinical trial. participants received two treatments at 30-minute intervals of either nebulized salbutamol (0.10 mg/kg in 2 ml 0.9% saline solution) or a similar volume of 0.9% saline solution placebo. outcome measures were the respiratory rate, pulse oximetry, and a ... | 1991 | 2019938 |
| management of the infant with respiratory syncytial virus. | this article examines updated clinical information concerning respiratory syncytial virus (rsv) infection including epidemiology, pathology, clinical manifestations, diagnosis, treatment, nosocomial infection, and prognosis. also presented is current information on ribavirin therapy, its side effects, and precautions. research related to the most effective isolation methodology is discussed, as well as nursing diagnoses based on gordon's functional health patterns and interventions for the infan ... | 1991 | 2019971 |
| human and bovine respiratory syncytial virus: immunopathologic mechanisms. | human respiratory syncytial virus (hrsv) is the major respiratory tract pathogen of infants and young children. bovine respiratory syncytial virus (brsv) is recognised as an important cause of respiratory tract disease in calves. both of these viruses and their respective diseases share many similarities. immunopathologic mechanisms have been proposed to be involved in the pathogenesis of respiratory syncytial virus (rsv) infections. this review examines the current understanding of the role of ... | 1991 | 2021054 |
| risk factors for respiratory syncytial virus-associated lower respiratory illnesses in the first year of life. | the relation of breast feeding and other factors to the incidence of respiratory syncytial virus-associated lower respiratory tract illness (rsv-lri) in the first year of life is examined. the study population is 1,179 healthy infants enrolled at birth between may 1980 and january 1984 into the tucson children's respiratory study, tucson, arizona. each subject's data were assessed at each month of age during the first year of life, during those months when respiratory syncytial virus was isolate ... | 1991 | 2035517 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. | a shell vial technique was used to recover respiratory syncytial virus (rsv) from frozen nasopharyngeal specimens previously tested by rapid diagnostic methods. with specimens determined to be positive by direct fluorescence assay (dfa), the shell vial technique was at least as sensitive as conventional tissue culture (92 versus 90%). the majority of rsv isolates were detected within 16 h postinoculation, versus an average of 4.5 days by conventional techniques. also, the shell vial method recov ... | 1991 | 2037662 |
| antigenic characterization of respiratory syncytial virus group a and b isolates in rio de janeiro, brazil. | the antigenic characteristics of 87 strains of respiratory syncytial virus isolated in rio de janeiro, brazil, from 1982 to 1988 were determined with a panel of monoclonal antibodies (mabs) in an enzyme immunoassay. four of these mabs immunoprecipitated the fusion protein, and five immunoprecipitated the large glycoprotein. on the basis of the patterns of reaction of these mabs to respiratory syncytial virus isolates in an enzyme immunoassay, we were able to separate isolates into the two major ... | 1991 | 2037672 |
| comparison of two rapid methods for detection of respiratory syncytial virus (rsv) (testpack rsv and ortho rsv elisa) with direct immunofluorescence and virus isolation for the diagnosis of pediatric rsv infection. | the ability of two commercial immunoassays to detect respiratory syncytial virus (rsv) in respiratory specimens was evaluated as follows: 152 specimens were tested by testpack rsv (abbott), and 72 were tested by ortho rsv elisa (ortho). test outcomes were compared with those of virus isolation alone, direct immunofluorescence assay (dfa) alone, or virus isolation and/or dfa. testpack rsv versus virus isolation showed 91% sensitivity, 96% specificity, 93% positive predictive value (ppv), and 95% ... | 1991 | 2037684 |
| effect of passive antibody on the immune response of cotton rats to purified f and g glycoproteins of respiratory syncytial virus (rsv). | the effect of passively transferred rsv immune serum on the antibody response to a single dose of purified rsv fusion (f) and large (g) glycoproteins was studied in cotton rats. passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eightfold suppression of the neutralizing antibody response of cotton rats to low doses of purified f and g glycoproteins (0.2-1.7 micrograms) and a twofold suppression to higher doses ... | 1991 | 2042390 |
| respiratory syncytial virus--from chimps with colds to conundrums and cures. | 1991 | 2046710 | |
| rapid identification of respiratory syncytial virus infections by direct fluorescent antibody testing: reliability as a guide to patient cohorting. | this study compared the results of a commercially available, direct fluorescent antibody (dfa) test with viral culture in 880 specimens obtained from 690 patients by means of nasopharyngeal swabs. the two tests were congruent in 92.5% (814) of the specimens. the sensitivity of the dfa was 0.95, the specificity was 0.91, the positive predictive value was 0.82, and the negative predictive value was 0.98. among 548 inpatients, there were 3 mixed infections (rsv and another virus), 8 rsv infections ... | 1991 | 2053715 |
| group-specific serum antibody responses in children with primary and recurrent respiratory syncytial virus infections. | antigenic group-specific serum antibody responses to first and second respiratory syncytial virus (rsv) infections were studied in children who had been followed longitudinally from early infancy in a research day-care center. plaque-reduction neutralizing (prn) antibody assays and elisas for the fusion (f) and attachment (g) glycoproteins were done using antigens of prototype rsv strains from groups a and b. responses to antigens of viruses homologous and heterologous to the antigenic group of ... | 1991 | 2056202 |
| [cytology of the bronchoalveolar lavage fluid in acute focal pneumonia]. | 1991 | 2057846 | |
| a survey of nosocomial respiratory viral infections in a children's hospital: occult respiratory infection in patients admitted during an epidemic season. | to define the extent of shedding of respiratory viruses and mycoplasma pneumoniae among a population of pediatric patients admitted to the hospital during a winter epidemic period and to identify nosocomial infections within this population. | 1991 | 2061581 |
| evaluation of an enzyme membrane immunoassay (directigen rsv) for the rapid diagnosis of respiratory syncytial virus infections. | a rapid enzyme immunoassay (eia) membrane test, the directigen respiratory syncytial virus (becton dickinson), was compared with cell culture, an indirect immunofluorescence (if) test, the monofluokit respiratory syncytial virus (diagnostics pasteur), and a conventional enzyme immunoassay antigen test, the abbott respiratory syncytial virus enzyme immunoassay in nasal aspirates specimens from children with suspected respiratory syncytial virus (rsv) bronchiolitis. the sensibility and specificity ... | 1991 | 2062553 |
| prevalence of respiratory syncytial virus subgroups a and b in france from 1982 to 1990. | a fluorescence antibody test with monoclonal antibodies was used to determine the subgroup (a or b) of respiratory syncytial virus from infants hospitalized in caen, france, over eight consecutive epidemics from 1982 to 1990. from 1982 to 1985, 27 (30%) out of 90 frozen nasal slides were classified as subgroup a strains and 63 (70%) were classified as subgroup b. b strains predominated over a in 1983-1984 and 1984-1985. from 1985 to 1990, 284 respiratory syncytial virus field strains were reisol ... | 1991 | 2064681 |
| isolation and characterisation of a porpoise morbillivirus. | a morbillivirus was isolated from lung tissue of a porpoise which had lesions similar to those of phocine distemper. porpoise and seal isolates differed in their reactions to monoclonal antibodies to canine distemper virus. | 1991 | 2069505 |
| respiratory syncytial virus infection in c57bl/6 mice: clearance of virus from the lungs with virus-specific cytotoxic t cells. | we describe respiratory syncytial virus (rsv)-specific cytotoxic t-cell (ctl) lines and clones developed from the spleens of c57bl/6 and balb/c mice. line 7 and clones derived from it were h-2kb restricted, whereas line 12 had both kb and db components. both lines, and all the clones except one, could lyse targets infected with either strain a or strain b rsv. line 7 or 7-11e1 cells (8 x 10(6) to 10 x 10(6) given intravenously cleared rsv from the lungs of infected mice. there was no morbidity o ... | 1991 | 2072460 |