Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| in-vitro antibacterial, antifungal and cytotoxic activities of some coumarins and their metal complexes. | a series of new antibacterial and antifungal coumarin-derived compounds and their transition metal complexes [cobalt (ii), copper (ii), nickel (ii) and zinc (ii)] have been synthesized, characterized and screened for their in vitro antibacterial activity against escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebacterium diphtheriae, staphylococcus aureus and streptococcus pyogenes bacterial strains ... | 2005 | 16206827 |
| quantitative assessment of phytopathogenic fungi in various substrates using a dna macroarray. | detection, identification and quantification of plant pathogens are the cornerstones of preventive plant disease management. to detect multiple pathogens in a single assay, dna array technology currently is the most suitable technique. however, for sensitive detection, polymerase chain reaction (pcr) amplification before array hybridization is required. to evaluate whether dna array technology can be used to simultaneously detect and quantify multiple pathogens, a dna macroarray was designed and ... | 2005 | 16232285 |
| evaluation of fusarium solani hyphae and conidia susceptibility to amphotericin b and itraconazole: study of a clinical case. | fusarium species are hyaline moulds belonging to the hyalohyphomycosis group that are usually found in the soil and plants. this organism has emerged as a cause of disseminated invasive disease. the correlation between in vitro value and clinical efficacy is low and many patients remain unresponsive to treatment despite in vitro susceptibility. we determined growth control for fusarium solani using the biocell-tracer system that measures the growth rate of a single fungal hypha, and the effect o ... | 2005 | 16244897 |
| disinfecting action of a new multi-purpose disinfection solution for contact lenses. | the disinfection activity of a new multipurpose disinfection solution (opti-free express with aldox) was compared to several other contact lens disinfecting solutions. the new solution is preserved with polyquaternium-1 and myristamidopropyl dimethylamine. the other solutions included 3% hydrogen peroxide systems and multipurpose solutions (mps) preserved with polyhexamethylene biguanide. the products were tested for antimicrobial activity against staphylococcus spp., pseudomonas aeruginosa, ser ... | 1999 | 16303414 |
| comparative antimicrobial efficacy of multi-purpose hydrogel lens care solutions. | ten single-bottle multipurpose hydrogel lens care solutions commercially available in the european market were evaluated for antimicrobial efficacy using the international organization for standardization's (iso) stand-alone procedure. the results of this study indicate that renu multi plus renu multi purpose, complete and dua meet the iso stand-alone primary acceptance criteria for stand-alone disinfectants against all challenge organisms: staphylococcus aureus, serratia marcescens, pseudomonas ... | 2001 | 16303461 |
| anatomy of a regimen: consideration of multipurpose solutions during non-compliant use. | consumers are often non-compliant with instructions for contact lens care products. this study explores the antimicrobial capacity of multipurpose solutions using variable use conditions. opti-free express (alcon) multi-purpose disinfecting solution with polyquad (alcon) and aldox (alcon) antimicrobial system and products containing phmb (renu multiplus, solo-care, and complete multipurpose solutions) were evaluated. products were challenged with fusarium solani, candida albicans, serratia marce ... | 2003 | 16303493 |
| contribution of regimen steps to disinfection of hydrophilic contact lenses. | the effect of regimen steps on overall performance of contact lens disinfection processes was evaluated. hydrophilic lenses were inoculated with staphylococcus aureus, pseudomonas aeruginosa, serratia marcescens, candida albicans, or fusarium solani. contributions of regimen steps and rinse volumes to disinfection abilities of opti-free express, solo-care plus (ciba vision), complete (allergan), and renu multiplus (bausch & lomb) multi-purpose solutions were examined. numbers of surviving microo ... | 2004 | 16303537 |
| effect of voriconazole combined with micafungin against candida, aspergillus, and scedosporium spp. and fusarium solani. | effects of voriconazole combined with micafungin against 101 isolates of candida spp. and 100 isolates of filamentous fungi have been evaluated by in vitro checkerboard analysis. the combination was indifferent for 97% of the candida isolates and synergistic for 64% of the filamentous fungi (79% for aspergillus fumigatus). | 2005 | 16304192 |
| methyl tert-butyl ether and tert-butyl alcohol degradation by fusarium solani. | fusarium solani degraded methyl tert-butyl ether (mtbe) and other oxygenated compounds from gasoline including tert-butyl alcohol (tba). the maximum degradation rate of mtbe was 16 mg protein h and 46 mg/g protein h for tba. the culture transformed 77% of the total carbon to 14co2. the estimated yield for mtbe was 0.18 g dry wt/g mtbe. | 2005 | 16314973 |
| synthesis and evaluation of demethoxyviridin derivatives as potential antimicrobials. | the in vitro antibacterial and antifungal activities of demethoxyviridin and some synthetic analogues were evaluated by the agar diffusion method. the minimum inhibitory concentrations (mic) of the active compounds were also determined by the agar dilution method. demethoxyviridin (1) showed moderate antibacterial activity against most of the strains tested. 1alpha-hydroxydemethoxyviridin (3) showed antibacterial activity and the most potent in vitro antifungal activity with mic of 20 microg/ml ... | 2005 | 16320609 |
| substrate specificity and kinetic properties of enzymes belonging to the hormone-sensitive lipase family: comparison with non-lipolytic and lipolytic carboxylesterases. | we have studied the kinetics of hydrolysis of triacylglycerols, vinyl esters and p-nitrophenyl butyrate by four carboxylesterases of the hsl family, namely recombinant human hormone-sensitive lipase (hsl), est2 from alicyclobacillus acidocaldarius, afest from archeoglobus fulgidus, and protein rv1399c from mycobacterium tuberculosis. the kinetic properties of enzymes of the hsl family have been compared to those of a series of lipolytic and non-lipolytic carboxylesterases including human pancrea ... | 2005 | 16325466 |
| degradation of c-labeled lignins and c-labeled aromatic acids by fusarium solani. | abilities of isolate af-w1 of fusarium solani to degrade the side chain and the ring structure of synthetic dehydrogenative polymerizates, aromatic acids, or lignin in sound wood were investigated under several conditions of growth substrate or basal medium and ph. significant transformations of lignins occurred in 50 days in both unextracted and extracted sound wood substrates with 3% malt as the growth substrate and the ph buffered initially at 4.0 with 2,2-dimethylsuccinate. degradation of li ... | 1980 | 16345616 |
| microbiological systems in organic synthesis: preparative-scale resolution of (rs)-glaucine by fusarium solani and stereospecific oxidation of (r)-(-)-glaucine by aspergillus flavipes. | the destructive resolution of (6ar,s)-glaucine (ic) was accomplished by oxidation of the (6as)-(+)-enantiomer (ia), using fusarium solani atcc 12823 to yield the unnatural alkaloid (6ar)-(-)-glaucine (ib). eighteen cultures were examined for their ability to metabolize the (6ar)-(-)-enantiomer (ib), and aspergillus flavipes atcc 1030 was found to catalyze the stereoselective oxidation of this substrate to didehydroglaucine. thus, it has been demonstrated that "r" and "s" organisms exist with reg ... | 1981 | 16345776 |
| fungal catabolism of crown gall opines. | this study was conducted to determine the capacities of 37 fungi to utilize various crown gall opines as their sole carbon and nitrogen source. one strain of fusarium solani, two of cylindrocarpon destructans, and six of cylindrocarpon heteronema catabolized octopine, mannopine, octopinic acid, succinamopine, or a combination of these opines. one c. heteronema and one fusarium dimerum strain grew only on succinamopine. none of the fungal isolates had the ability to grow on nopaline. the cataboli ... | 1990 | 16348087 |
| adhesion of macroconidia to the plant surface and virulence of nectria haematococca. | to study spore attachment of the cucurbit pathogen nectria haematococca (anamorph, fusarium solani f. sp. cucurbitae), mutants with adhesion-deficient macroconidia were isolated. the adhesion-deficient mutants were selected after treatment with n-methyl-n' -nitro-n-nitrosoguanidine followed by repeated enrichment for macroconidia which did not attach to polystyrene. two independently derived mutants produced macroconidia with an approximately 50% reduction in attachment to polystyrene and to zuc ... | 1990 | 16348379 |
| pseudomonas stutzeri ypl-1 genetic transformation and antifungal mechanism against fusarium solani, an agent of plant root rot. | an actively antagonistic bacterium that could be used as a biocontrol agent against fusarium solani, which causes root rots with considerable losses in many important crops, was isolated from a ginseng rhizosphere and identified as a strain of pseudomonas stutzeri. in several biochemical tests with culture filtrates of p. stutzeri ypl-1 and in mutational analyses of antifungal activities of reinforced or defective mutants, we found that the anti-f. solani mechanism of the bacterium may involve a ... | 1991 | 16348417 |
| influence of potassium in the agar medium on the growth pattern of the filamentous fungus fusarium solani. | a decrease in the concentration of k ions below 3 mm in agar medium which also contained starch, casein hydrolysate, mgso(4), and k(2)hpo(4) changed the growth pattern of fusarium solani illuminated in diurnal 12-h light/12-h dark cycles from zonation to a feathery growth mode. rubidium or cesium ions could replace potassium, but lithium, sodium, and the bivalent alkaline earth ions could not. | 1991 | 16348571 |
| genetic and biochemical characterization of nectria haematococca strains with adhesive and adhesion-reduced macroconidia. | a previous study reported the isolation of two mutants (le1 and le2) of the plant pathogenic fungus nectria haematococca (anamorph, fusarium solani f. sp. cucurbitae) with macroconidia with reduced ability to adhere (att) to zucchini fruits and polystyrene. the adhesion-reduced-phenotype in le1 and le2 macroconidia is temperature sensitive and dependent on the concentration of nutrients. classical genetic analysis of progeny derived from le1 identified a mutation in a genetic locus, named att1. ... | 1994 | 16349181 |
| antifungal effects and mechanism of action of viscotoxin a3. | viscotoxins are cationic proteins, isolated from different mistletoe species, that belong to the group of thionins, a group of basic cysteine-rich peptides of approximately 5 kda. they have been shown to be cytotoxic to different types of cell, including animal, bacterial and fungal. the aim of this study was to obtain information on the cell targets and the mechanism of action of viscotoxin isoform a3 (vta3). we describe a detailed study of viscotoxin interaction with fungal-derived model membr ... | 2006 | 16367749 |
| analysis of essential carboxylic amino acid residues for catalytic activity of fungal chitosanases by site-directed mutagenesis. | a comparison of amino acid sequences of fungal chitosanases, belonging to family 75 of glycosyl hydrolases, revealed three carboxylic amino acid residues completely conserved among all of the chitosanases. to study the role of these residues in catalysis, they were replaced with other residues by site-directed mutagenesis in the chitosanase gene of fusarium solani. the mutated genes were expressed in the yeast saccharomyces cerevisiae and the resulting recombinant chitosanases were used in kinet ... | 2005 | 16384794 |
| antifungal efficacy of chitosan and its thiourea derivatives upon the growth of some sugar-beet pathogens. | chitosan (cs) was modified by reaction with benzoyl thiocyanate to give a thiourea derivative (tucs). the antifungal behavior of chitosan and its thiourea derivative was investigated in vitro on the mycelial growth, sporulation and germination of conidia or sclerotia of the following sugar-beet: beta vulgaris pathogens isolated in egypt, rhizoctonia solani kühn (ag(2-2)) sclerotium rolfsii sacc. and fusarium solani (mart.) sacc. all the prepared thiourea derivatives had a significant inhibiting ... | 2006 | 16413607 |
| hexadecylphosphocholine (miltefosine) has broad-spectrum fungicidal activity and is efficacious in a mouse model of cryptococcosis. | the alkyl phosphocholine drug miltefosine is structurally similar to natural substrates of the fungal virulence determinant phospholipase b1 (plb1), which is a potential drug target. we determined the mics of miltefosine against key fungal pathogens, correlated antifungal activity with inhibition of the plb1 activities (plb, lysophospholipase [lpl], and lysophospholipase-transacylase [lpta]), and investigated its efficacy in a mouse model of disseminated cryptococcosis. miltefosine inhibited sec ... | 2006 | 16436691 |
| herbicidal and fungicidal activities of lactones in kava (piper methysticum). | this is the first report showing that kava lactones are plant and plant fungus growth inhibitors. aqueous extract of kava roots showed high allelopathic potential and strongly suppressed germination and growth of lettuce, radish, barnyardgrass, and monochoria. nine kava lactones were detected using gc-ms including desmethoxyyagonin, kavain, 7,8-dihydrokavain, hydroxykavain, yagonin, 5,6,7,8-tetrahydroxyyagonin, methysticin, dihydromethysticin, and 11-hydroxy-12-methoxydihydrokavain. quantities o ... | 2006 | 16448174 |
| versatile expression and secretion vectors for bacillus subtilis. | most expression systems are based on escherichia coli as the host strain because of the large availability of all kinds of vector plasmids. however, aside from the obvious advantages of e. coli systems, serious problems can occur during the process of heterologous gene expression and purification. therefore, low expression rates, formation of inclusion bodies, improper protein-folding, and/or toxicity problems might enforce changing the expression host. here we describe the construction of two n ... | 2006 | 16450069 |
| deoxycholate amphotericin b and amphotericin b lipid complex exert additive antifungal activity in combination with pulmonary alveolar macrophages against fusarium solani. | fusarium spp. have emerged as important causes of invasive fungal infections in immunocompromised patients. rabbit pulmonary alveolar macrophages (pams) exhibited fungicidal activity against conidia of fusarium solani and achieved a time-dependent increase in killing. neither deoxycholate amphotericin b (damb) nor amphotericin b lipid complex (ablc) exerted a suppressive effect on pams by decreasing their conidiocidal activity against f. solani. on the contrary, at a concentration of 0.125 micro ... | 2006 | 16466443 |
| variability of non-mutualistic filamentous fungi associated with atta sexdens rubropilosa nests. | a survey of the filamentous fungi other than the symbiotic one found in association with atta sexdens rubropilosa colonies was carried out. different fungal species (27 taxa) were isolated a few days after treating the workers with toxic baits (sulfluramid; mirex-s), from 40 laboratory and 20 field nests. syncephalastrum racemosum (54%) and escovopsis weberi (21%), trichoderma harzianum (38%) and fusarium oxysporum (23%) were the prevalent species in laboratory and field nests, respectively. acr ... | 2005 | 16475502 |
| head-to-head comparison of the activities of currently available antifungal agents against 3,378 spanish clinical isolates of yeasts and filamentous fungi. | we have compared the activities of posaconazole and other currently available antifungal agents against a collection of 3,378 clinical isolates of yeasts and filamentous fungi. a total of 1,997 clinical isolates of candida spp., 359 of other yeast species, 697 strains of aspergillus spp., and 325 nondermatophyte non-aspergillus spp. were included. the average geometric means of the mics of agents that were tested against candida spp. were 0.23 microg/ml for amphotericin b, 0.29 microg/ml for flu ... | 2006 | 16495251 |
| cyanide degradation under alkaline conditions by a strain of fusarium solani isolated from contaminated soils. | several cyanide-tolerant microorganisms have been selected from alkaline wastes and soils contaminated with cyanide. among them, a fungus identified as fusarium solani ihem 8026 shows a good potential for cyanide biodegradation under alkaline conditions (ph 9.2 to 10.7). results of k(sup14)cn biodegradation studies show that fungal metabolism seems to proceed by a two-step hydrolytic mechanism: (i) the first reaction involves the conversion of cyanide to formamide by a cyanide-hydrolyzing enzyme ... | 1997 | 16535647 |
| effect of the high polycyclic aromatic hydrocarbon, benzo[a]pyrene, on the lipid content of fusarium solani. | the purpose of the present paper was to study the effect of the high polycyclic aromatic hydrocarbon (pah), benzo[a]pyrene, on the lipid [fatty acid (fa) and sterol] composition and content of the fungi fusarium solani and f. oxysporum, respectively recognized as good and poor pah degraders. the major fas and the major sterol that characterized the tested fusarium strains were c16:0, c18:1, c18:2, and ergosterol. lipid profiles of f. solani remained unchanged with the addition of benzo[a]pyrene ... | 2006 | 16546362 |
| oxovanadium(iv) complexes of hydrazides: potential antifungal agents. | oxovanadium(iv) -derived antifungals have been prepared by condensing equimolar amounts of vanadyl sulfate with hydrazides. all the synthesized ligands and their metal complexes were characterized by ir, uv-visible and micro analytical data. these synthesized compounds were screened for their antifungal activity against aspergillus flavus (a. flavus), trichophyton longifusus (t. longifusus), candida albicans (c. albicans), microsporum canis (m. canis), fusarium solani (f. solani) and candida gla ... | 2006 | 16570503 |
| in vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes. | isonicotinoylhydrazide schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their co (ii), cu (ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against mycobacterium tuberculosis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebac ... | 2006 | 16570512 |
| mechanism by which contact with plant cuticle triggers cutinase gene expression in the spores of fusarium solani f. sp. pisi. | spores of the phytopathogenic fungus fusarium solani f. sp. pisi were shown to produce the extracellular enzyme, cutinase, only when cutin or cutin hydrolysate was added to the spore suspension. dihydroxy-c(16) acid and trihydroxy-c(18) acid, which are unique cutin monomers, showed the greatest cutinase-inducing activity. experiments with several compounds structurally related to these fatty acids suggested that both a omega-hydroxyl and a midchain hydroxyl are required for cutinase-inducing act ... | 1986 | 16593666 |
| the causal agents of damping-off disease of buglosse from iran. | iran is considered a major genetic for medicinal plant in the world. because of this significant diversity and historical background in identification and utilization to remedy human and animal diseases, export of medicinal plant can help to strengthen local as well as natural economy. buglosse (fig. 1) is one of the most important and common medicinal plants in iran and exist as echium amoneum and borago officinalis. this work was conducted in order to identify the causal agent(s) of damping of ... | 2005 | 16637194 |
| reaction of selected soybean cultivars to rhizoctonia root rot and other damping-off disease agents. | eight soybean cultivars; giza 21. giza 22, giza 35, giza 82, giza 83, crawford, holladay and toamo were evaluated to rhizoctonia root rot using agar plate and potted plant techniques. data cleared that, in agar plate assay all soybean cultivars were moderately susceptible (ms), although the differences between them were significant (p=0.05). generally, in potted assay, the reactions were resistant (r) or moderately resistant (mr) to root rots. also, the differences between cultivars were signifi ... | 2005 | 16637203 |
| gaseous hexane biodegradation by fusarium solani in two liquid phase packed-bed and stirred-tank bioreactors. | biofiltration of hydrophobic volatile pollutants is intrinsically limited by poor transfer of the pollutants from the gaseous to the liquid biotic phase, where biodegradation occurs. this study was conducted to evaluate the potential of silicone oil for enhancing the transport and subsequent biodegradation of hexane by the fungus fusarium solani in various bioreactor configurations. silicone oil was first selected among various solvents for its biocompatibility, nonbiodegradability, and good par ... | 2006 | 16646479 |
| spore germination and carbon metabolism in fusarium solani. iii. carbohydrate respiration in relation to germination. | 1963 | 16655828 | |
| spore germination and carbon metabolism in fusarium solani v. changes in anaerobic metabolism and related enzyme activities during development. | macroconidia of fusarium solani f. phascoli have no detectable capacity to respire glucose anaerobically; germinated spores and mycelium, on the other hand, ferment glucose, although slowly.extracts of ungerminated spores contain hexokinase, phosphohexoisomerase, phosphofructokinase, aldolase, triose phosphate dehydrogenase, triose phosphate isomerase, phosphoglyceric kinase, enolase, phosphoglyceric mutase, pyruvate kinase, and pyruvate decarboxylase. it follows, therefore, that the appearance ... | 1966 | 16656324 |
| physiological and cytological similarities between disease resistance and cellular incompatibility responses. | excised pea pods responded similarly to both the invasion of plant pathogenic fungi and the presence of bean tissue, bean pollen, and mouse tumor cells by synthesizing pisatin and by developing a characteristic yellow-green fluorescence. both responses were dependent on rna and protein synthesis. conversely, the foreign pollen and incompatible fungi were sensitive to the pea pod tissue and were subject to abnormal development.the induction of pisatin and the yellow-green fluorescence development ... | 1974 | 16658953 |
| proof for the production of cutinase by fusarium solani f. pisi during penetration into its host, pisum sativum. | rabbit antibody to cutinase-i, isolated from fusarium solani f. pisi, was conjugated to ferritin. with this ferritin-conjugated antibody it was shown that germinating spores of this fungus excreted cutinase during the penetration of the host pisum sativum. this result constitutes the most specific and strongest evidence for an enzymic penetration of a plant cuticle by a pathogen during infection. | 1977 | 16660031 |
| glycosidic enzyme activity in pea tissue and pea-fusarium solani interactions. | membrane barriers which prevent direct contact between fusarium solani and pea endocarp tissue prevent fungal spores from inducing phytoalexin production. conversely, preinduced host resistance responses are not readily transported from the plant across the membrane barrier to fusarium macroconidia.crude enzyme extracts from pea endocarp tissues partially degrade fusarium solani f. sp. phaseoli cell walls. activities of the glycosidic enzymes, chitinase, beta-1,3-glucanase, chitosanase, beta-d-n ... | 1980 | 16661404 |
| chitosan as a component of pea-fusarium solani interactions. | chitosan, a polymer of beta-1,4-linked glucosamine residues with a strong affinity for dna, was implicated in the pea pod-fusarium solani interaction as an elicitor of phytoalexin production, an inhibitor of fungal growth and a chemical which can protect pea tissue from infection by f. solani f. sp. pisi. purified fusarium fungal cell walls can elicit phytoalexin production in pea pod tissue. enzymes from acetone powders of pea tissue release eliciting components from the f. solani f. sp. phaseo ... | 1980 | 16661405 |
| localization of fungal components in the pea-fusarium interaction detected immunochemically with anti-chitosan and anti-fungal cell wall antisera. | antisera specific for purified cell walls of fusarium solani f. sp. pisi and phaseoli and of shrimp shell chitosan were utilized as immunochemical probes to determine the location of fungal components in the pea-fusarium interaction.within 15 minutes after inoculation, fungal cell wall components appear to enter the plant cell and to accumulate inside the plant cell wall as fungal growth on the plant tissue is inhibited. the accumulation patterns of chitosan and all components containing hexosam ... | 1981 | 16661621 |
| effects of light and of fusarium solani on synthesis and activity of phenylalanine ammonia-lyase in peas. | phenylalanine ammonia-lyase was purified from peas, and a specific antiserum against the enzyme was produced in rabbits. the antiserum was used to study the first 8 hours of the phenylalanine ammonia-lyase activity response in two different organs of the pea from different developmental stages and in response to two different stimuli. etiolated seedlings were pulse-labeled with l-[(35)s]methionine after either no light exposure or after specific periods of irradiation with blue light. immature p ... | 1981 | 16661979 |
| ethylene: symptom, not signal for the induction of chitinase and beta-1,3-glucanase in pea pods by pathogens and elicitors. | infection of immature pea pods with fusarium solani f.sp. phaseoli (a non-pathogen of peas) or f.sp. pisi (a pea pathogen) resulted in induction of chitinase and beta-1,3-glucanase. within 30 hours, activities of the two enzymes increased 9-fold and 4-fold, respectively. chitinase and beta-1,3-glucanase were also induced by autoclaved spores of the two f. solani strains and by the known elicitors of phytoalexins in pea pods, cadmium ions, actinomycin d, and chitosan. furthermore, exogenously app ... | 1984 | 16663892 |
| antifungal hydrolases in pea tissue : i. purification and characterization of two chitinases and two beta-1,3-glucanases differentially regulated during development and in response to fungal infection. | chitinase and beta-1,-3-glucanase activities increased coordinately in pea (pisum sativum l. cv "dot") pods during development and maturation and when immature pea pods were inoculated with compatible or incompatible strains of fusarium solani or wounded or treated with chitosan or ethylene. up to five major soluble, basic proteins accumulated in stressed immature pods and in maturing untreated pods. after separation of these proteins by chromatofocusing, an enzymic function could be assigned to ... | 1988 | 16666142 |
| antifungal hydrolases in pea tissue : ii. inhibition of fungal growth by combinations of chitinase and beta-1,3-glucanase. | chitinase and beta-1,3-glucanase purified from pea pods acted synergistically in the degradation of fungal cell walls. the antifungal potential of the two enzymes was studied directly by adding protein preparations to paper discs placed on agar plates containing germinated fungal spores. protein extracts from pea pods infected with fusarium solani f.sp. phaseoli, which contained high activities of chitinase and beta-1,3-glucanase, inhibited growth of 15 out of 18 fungi tested. protein extracts f ... | 1988 | 16666407 |
| post-traumatic fungal endophthalmitis--a prospective study. | to study the incidence, clinical presentation, and the response of anti-fungals in cases of fungal endophthalmitis following open globe injury. | 2008 | 16751752 |
| members of the fusarium solani species complex that cause infections in both humans and plants are common in the environment. | members of the fusarium solani species complex (fssc) are increasingly implicated as the causative agents of human mycoses, particularly in the expanding immunocompromised and immunosuppressed patient populations. best known as ubiquitous plant pathogens and saprotrophs, the fssc comprises over 45 phylogenetically distinct species distributed among three major clades. to identify which species are associated with human infections, we generated multilocus haplotypes based on four partial gene seq ... | 2006 | 16757619 |
| insurgence of fusarium keratitis associated with contact lens wear. | to describe the clinical presentation and course of patients who developed keratitis due to fusarium while wearing nontherapeutic soft contact lenses. | 2006 | 16769827 |
| de novo design, synthesis and screening of a combinatorial library of complementary ligands directed towards the surface of cutinase from fusarium solani pisi. | the protein surface is the interface through which a protein molecule senses the external world. the composition of this interface, in charged, polar and/or hydrophobic residues is crucial for both the activity and stability of the protein. protein immobilization on surfaces has been extensively explored as one of the most effective approaches for stabilization. the mechanism of stabilization, however, is still poorly understood, and usually the success of any method is more a matter of trial an ... | 2006 | 16779873 |
| [clinical and microbiological study of mycetomas at the muñiz hospital of buenos aires between 1989 and 2004]. | this work presents clinical, microbiological and outcome data collected from 76 patients with mycetomas at the muñiz hospital from 1989 to 2004. forty-nine patients were male and 27 female; the mean age was 43.4 years. the majority of the patients acquired the infection in argentina: the most affected provinces were santiago del estero with 31 cases, and chaco with 11; 8 cases came from other countries (bolivia 6 and paraguay 2). the mean evolution of the disease was 9.2 years. the most frequent ... | 2006 | 16784127 |
| studies on kinetics and thermostability of a novel acid invertase from fusarium solani. | the present investigation deals with purification and thermal characterization of an acid invertase produced by fusarium solani in submerged culture. the maximum enzyme activity (9.90 u ml(-1)) was achieved after 96 h of cultivation at ph 5.0 and 30 degrees c in a basal medium containing molasses (2%) as the carbon and energy source supplemented with 1% peptone. invertase was purified by ammonium sulfate fractionation and column chromatography on deae-cellulose and sephadex g-200. the purified e ... | 2006 | 16787006 |
| antibacterial, antifungal and cytotoxic properties of some sulfonamide-derived chromones. | a series of antibacterial and antifungal sulfonamide (sulfanilamide, sulfaguanidine, sulfamethaxozole, 4-aminoethylbenzene-sulfonamide and 4-amino-6-trifluoromethyl-benzene-1,3-disulfonamide) derived chromones, previously reported as inhibitors of carbonic anhydrase, have been screened for in-vitro antibacterial activity against four gram-negative (escherichia coli, pseudomonas aeruginosa, salmonella typhi and shigella flexener) and two gram-positive (bacillus subtilis and staphylococcus aureus) ... | 2006 | 16789431 |
| increase of the hydrophilicity of polyethylene terephthalate fibres by hydrolases from thermomonospora fusca and fusarium solani f. sp. pisi. | treatment of polyethylene terephthalate fibres with hydrolase preparations from thermomonospora (thermobifida) fusca and fusarium solani f. sp. pisi resulted in an increase of the hydrophilicity of the fibres determined by measurement of their dyeing behaviour with reactive dyes and their water absorption ability. reflectance spectrometry of treated fibres dyed with a reactive dye showed that the colour became more intense corresponding to an increase of hydroxyl groups on the fibre surfaces and ... | 2006 | 16791721 |
| antifungal activity and enhancement of plant growth by bacillus cereus grown on shellfish chitin wastes. | bacillus cereus qq308 produced antifungal hydrolytic enzymes, comprising chitinase, chitosanase and protease, when grown in a medium containing shrimp and crab shell powder (scsp) produced from marine waste. the growth of the plant-pathogenic fungi fusarium oxysporum, fusarium solani, and pythium ultimum were considerably affected by the presence of the qq308 culture supernatant. the supernatant inhibited spore germination and germ tube elongation of f. oxysporum, f. solani, and p. ultimum. the ... | 2007 | 16797180 |
| antimicrobial activity of potato aspartic proteases (staps) involves membrane permeabilization. | solanum tuberosum aspartic proteases (staps) with antimicrobial activity are induced after abiotic and biotic stress. in this study the ability of staps to produce a direct antimicrobial effect was investigated. viability assays demonstrated that staps are able to kill spores of fusarium solani and phytophthora infestans in a dose-dependent manner. localization experiments with fitc-labelled staps proved that the proteins interact directly with the surface of spores and hyphae of f. solani and p ... | 2006 | 16804179 |
| molecular characterization of a potato map kinase transcriptionally regulated by multiple environmental stresses. | the mapk cascade is an evolutionary conserved signaling pathway that links external stimuli with cellular responses. using polymerase chain reaction (pcr), a dna fragment corresponding to a solanum tuberosum mapk, stmpk1, was isolated. stmpk1 amino acid sequence displayed over 90% identity with tomato mpk1 (lempk1) and tobacco sipk. southern blot analysis indicated that the gene encoding stmpk1 is present in a single copy in the potato genome. stmpk1 mrna levels differentially accumulated in pot ... | 2006 | 16814558 |
| light is essential for degradation of ribulose-1,5-bisphosphate carboxylase-oxygenase large subunit during sudden death syndrome development in soybean. | fusarium solani f. sp. glycines (fsg) has been reported to produce at least two phytotoxins. cell-free fsg culture filtrates containing phytotoxins have been shown to develop foliar sudden death syndrome (sds) in soybean. we have investigated the changes in protein profiles of diseased leaves caused by cell-free fsg culture filtrates prepared from fsg isolates. two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (page) was conducted to investigate the protein profiles of di ... | 2006 | 16821191 |
| nutritive value, antioxidant and antimicrobial properties of struchium sparganophora leaves. | struchium sparganophora leaf is a green leafy vegetable popularly used in soup preparations in processed and unprocessed forms in nigeria. fresh leaves of s. sparganophora were soaked overnight to remove its bitterness, before determination of the nutrient and antinutrient content, while sun-dried s. sparganophora leaves were extracted with rectified spirit. the antioxidant and antimicrobial properties of the extracts were subsequently determined. the result of the study revealed that the fresh ... | 2006 | 16822216 |
| the role of chitosan in protection of soybean from sudden death syndrome caused by fusarium solani f. sp. glycines. | the in vitro antifungal properties of chitosan and its role in protection of soybean from a sudden death syndrome (sds) were evaluated. chitosan inhibited the radial and submerged growth of f. solani f. sp. glycines with a marked effect at concentrations up to 1mg/ml indicating antifungal property and at 3mg/ml was able to delay sds symptoms expression on soybean leaves for over three days after fungal inoculation when applied preventively. chitosan was able to induce the level of chitinase acti ... | 2007 | 16828285 |
| fsfks1, the 1,3-beta-glucan synthase from the caspofungin-resistant fungus fusarium solani. | the cell wall, a mesh of carbohydrates and proteins, shapes and protects the fungal cell. the enzyme responsible for the synthesis of one of the main components of the fungal wall, 1,3-beta-glucan synthase, is targeted by the antifungal caspofungin acetate (cfa). clinical isolates of candida albicans and aspergillus fumigatus are much more sensitive to cfa than clinical isolates of fusarium species. to better understand cfa resistance in fusarium species, we cloned and sequenced fsfks1, which en ... | 2006 | 16835448 |
| the interaction of fusarium solani pisi cutinase with long chain spin label esters. | we here present a study of the interaction between the fusarium solani pisi cutinase mutant s120a and spin-labeled 4,4-dimethyloxazoline-n-oxyl-(doxyl)-stearoyl-glycerol substrates in a micellar system. the interaction is detected by nmr measuring changes in chemical shift for 1h and 15n as well as relaxation parameters for backbone 1h (t1) and 15n (t1, t2) atoms as well as for side chain methyl groups 1h (t1). the detected interaction shows a weak binding of cutinase to the lipid micelles. stru ... | 2006 | 16866362 |
| [bilateral infectious keratitis in a patient wearing cosmetic soft contact lenses]. | we report a case of an immunocompetent 20-year-old woman, wearing planocosmetic contact lenses to change the color of her eyes, with no contact lens hygiene regimen. she developed a bilateral infectious keratitis. acanthamoeba and fusarium solani were isolated in both eyes. bilateral penetrating keratoplasty was needed because of bilateral corneal perforation. three months later, bilateral simultaneous phacoemulsification was performed because of a dense cataract and a severe decrease in her vis ... | 2006 | 16885898 |
| hyperinduction of nitrilases in filamentous fungi. | 2-cyanopyridine proved to act as a powerful nitrilase inducer in aspergillus niger k10, fusarium solani o1, fusarium oxysporum ccf 1414, fusarium oxysporum ccf 483 and penicillium multicolor ccf 2244. valeronitrile also enhanced the nitrilase activity in most of the strains. the highest nitrilase activities were produced by fungi cultivated in a czapek-dox medium with both 2-cyanopyridine and valeronitrile. the specific nitrilase activities of these cultures were two to three orders of magnitude ... | 2006 | 16909267 |
| a case of fusariosis in an immunocompromised patient successfully treated with liposomal amphotericin b. | although aspergillosis remains the most common mould infection in patients with haematologic malignancies, disseminated fusarium infection is an emerging problem with a poor prognosis in this patient population. the treatment options are limited due to relative resistance of the fungus to standard antifungals. we present a patient with acute lymphoblastic leukaemia successfully treated with ambisome for a disseminated fusarium solani infection that did not respond to first line treatment with vo ... | 2006 | 16918066 |
| cytotoxicity and antimicrobial activity of six multipurpose soft contact lens disinfecting solutions. | to assess and compare the cytotoxicity and antimicrobial activity of six multipurpose disinfecting contact lens solutions (mpds). | 2006 | 16918772 |
| modification of phenolic metabolism in soybean hairy roots through down regulation of chalcone synthase or isoflavone synthase. | soybean hairy roots, transformed with the soybean chalcone synthase (chs6) or isoflavone synthase (ifs2) genes, with dramatically decreased capacity to synthesize isoflavones were produced to determine what effects these changes would have on susceptibility to a fungal pathogen. the isoflavone and coumestrol concentrations were decreased by about 90% in most lines apparently due to gene silencing. the ifs2 transformed lines had very low ifs enzyme activity in microsomal fractions as measured by ... | 2007 | 16924535 |
| the effect of additives and mechanical agitation in surface modification of acrylic fibres by cutinase and esterase. | the surface of an acrylic fibre containing about 7% of vinyl acetate was modified using fusarium solani pisi cutinase and a commercial esterase, texazym pes. the effect of acrylic solvents and stabilising polyols on cutinase operational stability was studied. the half-life time of cutinase increased by 3.5-fold with the addition of 15% n,n-dimethylacetamide (dma) and by 3-fold with 1m glycerol. the impact of additives and mechanical agitation in the protein adsorption and in the hydrolysis of vi ... | 2006 | 16927260 |
| systematic screening of all signal peptides from bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria. | efficient protein secretion is very important in biotechnology as it provides active and stable enzymes, which are an essential prerequisite for successful biocatalysis. therefore, optimizing enzyme-producing bacterial strains is a major challenge in the field of biotechnology and protein production. in this study, the gram-positive model bacterium bacillus subtilis was optimized for heterologous protein secretion using a novel approach. two lipolytic enzymes, cutinase from fusarium solani pisi ... | 2006 | 16930615 |
| comparison of antifungal treatments for murine fusariosis. | fusarium solani infections are notoriously difficult to treat. we compared the efficacy of polyenes and an echinocandin in treating murine fusariosis to identify the optimal therapeutic regimen. | 2006 | 16973654 |
| [successful treatment with voriconazole for disseminated cutaneous and visceral infection by fusarium solani in a patient with acute myeloid leukemia]. | we report the successful treatment of a disseminated fusarium infection with skin manifestations in a severely neutropenic patient. a 51-year-old man with acute myeloblastic leukemia (m4) underwent two courses of remission induction therapy with cytarabine and daunorubicin. despite prophylactic treatment with tosufloxacin and micafungin, the patient developed a febrile scrotal ulcer. eight days later, we noted the appearance of painful and diffuse cutaneous nodules and a plain chest x-ray disclo ... | 2006 | 16986714 |
| antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents. | the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ... | 2006 | 16989308 |
| mild hydrolysis of nitriles by fusarium solani strain o1. | high levels of an aromatic nitrilase (about 37 microkat/l culture) were induced in fusarium solani o1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/l picolinonitrile. the mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (lentikats). the cell-free extract was immobilized by hydrophobic binding onto a butyl sepharose column. the enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonit ... | 2006 | 17007419 |
| phase partition of gaseous hexane and surface hydrophobicity of fusarium solani when grown in liquid and solid media with hexanol and hexane. | the filamentous fungus, fusarium solani, was grown in liquid and solid culture with glucose, glycerol, 1-hexanol and n-hexane. the partition coefficient with gaseous hexane (hpc) in the biomass was lower when grown in liquid medium with 1-hexanol (0.4) than with glycerol (0.8) or glucose (1) the hpc for surface growth were 0.2 for 1-hexanol, 0.5 for glycerol, 0.6 for glucose, and 0.2 for f. solani biomass obtained from a biofilter fed with gaseous n-hexane. these values show a 200-fold increase ... | 2006 | 17021662 |
| photophysics of the single tryptophan residue in fusarium solani cutinase: evidence for the occurrence of conformational substates with unusual fluorescence behaviour. | the single tryptophan residue, at position 69 in the amino acid sequence, was used as an intrinsic probe to obtain structural and dynamical information on the lipolytic enzyme fusarium solani cutinase. in the enzyme's native state the tryptophan fluorescence is highly quenched. time-resolved experiments reveal that the majority of the excited state species is characterized by an unusually fast decay time of approximately 40 ps, indicating the occurrence of a very efficient nonradiative relaxatio ... | 1996 | 17023358 |
| effect of aniline coupling on kinetic and thermodynamic properties of fusarium solani glucoamylase. | purified glucoamylase (ga) from fusarium solani was chemically modified by cross-linking with aniline hydrochloride in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (edc) for 1 [aniline-coupled glucoamylase-1 (acg-1)], 7 (acg-7), and 13 min (acg-13). the aniline coupling of ga had a profound enhancing effect on temperature, ph optima, and pk (a)'s of active site residues. the specificity constants (k (cat)/k (m)) of native, acg-1, acg-7, and acg-13 were 136, 244, 262, and 208 at ... | 2007 | 17031637 |
| systemic fungemia and hepatic localizations of fusarium solani in a liver transplanted patient: an emerging fungal agent. | the incidence of invasive fungal infection is increasing especially in the field of transplantation, affecting as many as 50% of bone marrow transplant (bmt) patients with neutropenia and 5-20% of solid-organ transplant (sot) recipients. fusarium species are soil saprophytes and plant pathogens. they may cause superficial mycoses or important opportunistic infections in patients with bone marrow suppression and neutropenia, they have been rarely described in solid organ recipients, and up to now ... | 2006 | 17058254 |
| report on testing from an investigation of fusarium keratitis in contact lens wearers. | recent reports of disproportionate outbreaks of microbial keratitis caused by fusarium solani have led to the removal of the bausch & lomb multipurpose lens care solution renu with moistureloc ("moistureloc") from the global market. previous studies have shown an increased risk of contact lens-related corneal infection in connection with overnight wear, noncompliant lens wearing and lens care behaviors, specific treatment regimens, and other patient-related and non-patient-related factors. the p ... | 2006 | 17099384 |
| biocide uptake in contact lenses and loss of fungicidal activity during storage of contact lenses. | with recent outbreaks of fusarium keratitis related to contact lens wear, studies were conducted to determine the biocide uptake during lens storage, and the resulting effect on antifungal activity of related products. | 2006 | 17099385 |
| purification and characterization of a lectin from endophytic fungus fusarium solani having complex sugar specificity. | a lectin from the mycelial extract of an endophytic strain of fusarium solani was purified. its hemagglutinating activity was inhibited by glycoproteins possessing n-linked as well as o-linked glycans. the thermodynamics and kinetics of binding of glycans and glycoproteins to f. solani lectin was studied using surface plasmon resonance. the lectin showed high affinity for asialofetuin, asialomucin, asialofibrinogen, and thyroglobulin; and comparatively low affinity for mucin, fetuin, fibrinogen, ... | 2007 | 17118333 |
| comparison of the hydrolysis of polyethylene terephthalate fibers by a hydrolase from fusarium oxysporum lch i and fusarium solani f. sp. pisi. | the hydrolysis of polyethylene terephthalate (pet) fibers by two fungal hydrolases was investigated. the hydrolase from a newly isolated fusarium oxysporum strain (lch 1) was more efficient in releasing terephthalic acid from pet fibers compared to the enzyme from f. solani f. sp. pisi dsm 62420 when equal amounts of p-nitrophenyl butyrate-hydrolyzing activity were employed. pet fabrics treated under the same conditions with the enzyme from f. oxysporum lch 1 also showed a considerably higher in ... | 2007 | 17136729 |
| deep cutaneous infection by fusarium solani in a healthy child: successful treatment with local heat therapy. | we describe a 6-year-old boy with a granulomatous lesion on the upper aspect of his left arm. fusarium solani infection was confirmed by biopsy studies and cultures. the biopsy specimen showed an unusually extensive dermal invasion with fungal hyphae. this is an uncommon clinical presentation for deep cutaneous infection caused by f solani in a healthy child. local heat therapy with a chemical pocket warmer led to dramatic healing of the lesion. | 2007 | 17147970 |
| in vivo confocal microscopy in fungal keratitis. | fungal keratitis is a major blinding eye disease found throughout the world, particularly in developing countries. given the recent increase in fusarium keratitis infections in contact lens wearers owing to contact lens solutions, a warning was recently issued by the food and drug administration, making it a public health concern in developed countries. | 2007 | 17151059 |
| cladosporium fulvum avr4 protects fungal cell walls against hydrolysis by plant chitinases accumulating during infection. | resistance against the leaf mold fungus cladosporium fulvum is mediated by the tomato cf proteins which belong to the class of receptor-like proteins and indirectly recognize extracellular avirulence proteins (avrs) of the fungus. apart from triggering disease resistance, avrs are believed to play a role in pathogenicity or virulence of c. fulvum. here, we report on the avirulence protein avr4, which is a chitin-binding lectin containing an invertebrate chitin-binding domain (cbm14). this domain ... | 2006 | 17153926 |
| development of a pooled probe method for locating small gene families in a physical map of soybean using stress related paralogues and a bac minimum tile path. | genome analysis of soybean (glycine max l.) has been complicated by its paleo-autopolyploid nature and conserved homeologous regions. landmarks of expressed sequence tags (ests) located within a minimum tile path (mtp) of contiguous (contig) bacterial artificial chromosome (bac) clones or radiation hybrid set can identify stress and defense related gene rich regions in the genome. a physical map of about 2,800 contigs and mtps of 8,064 bac clones encompass the soybean genome. that genome is bein ... | 2006 | 17156445 |
| growth and survival of fusarium solani-f. oxysporum complex on stressed multipurpose contact lens care solution films on plastic surfaces in situ and in vitro. | to analyze factors implicating the association of renu with moistureloc (renu ml) multipurpose contact lens solution (mps) with the increased incidence of fusarium keratitis. | 2006 | 17172900 |
| secretion of keratinolytic enzymes and keratinolysis by scopulariopsis brevicaulis and trichophyton mentagrophytes: regression analysis. | a survey on keratinophilic fungi from poultry-farm soils at namakkal and from feather dumping soils at chennai, india, revealed the existence of 34 species of fungi. most of the fungi exhibited variable efficiency in producing extracellular keratinase when grown in plates with chicken feathers as the sole carbon and nitrogen source. the fungi aspergillus flavus, aspergillus niger, aspergillus versicolor, chrysosporium state of arthroderma tuberculatum, paecilomyces carneus, scopulariopsis brevic ... | 2006 | 17215897 |
| universal in vitro antifungal resistance of genetic clades of the fusarium solani species complex. | eleven antifungal drugs were tested against representative isolates of the four phylogenetic clades of the fusarium solani species complex obtained in a multilocus sequence analysis. they all showed very poor activity, with no differences among the clades. amphotericin b was the most active drug. | 2007 | 17220423 |
| effects of a chitin-binding vicilin from enterolobium contortisiliquum seeds on bean bruchid pests (callosobruchus maculatus and zabrotes subfasciatus) and phytopathogenic fungi (fusarium solani and colletrichum lindemuntianum). | chitin-binding vicilin from enterolobium contortisiliquum seeds was purified by ammonium sulfate followed by gel filtration on sephacryl 300-sh and on sephacryl 200-sh. the vicilin, called ecv, is a dimeric glycoprotein composed of 1.03% carbohydrates and a mr of 151 kda, consisting of two subunits of mr of 66.2 and 63.8 kda. the ecv homogeneity was confirmed in a page where it was observed to be a unique acid protein band with slow mobility in this native gel. e. contortisiliquum vicilin (ecv) ... | 2007 | 17227051 |
| in-vitro antibacterial, antifungal and cytotoxic activity of cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes with furanylmethyl- and thienylmethyl-dithiolenes: [1, 3-dithiole- 2-one and 1,3-dithiole-2-thione]. | some antibacterial and antifungal furanylmethyl-and thienylmethyl dithiolenes and, their co(ii), cu(ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against four gram-negative; escherichia coli, pseudomonas aeruginosa, salmonella typhi and shigella flexeneri, and two gram-positive; bacillus subtilis and staphylococcus aureus bacterial strains, and for in-vitro antifungal activity against trichophyton longifusus, candid ... | 2006 | 17252947 |
| in-vitro antibacterial, antifungal and cytotoxic properties of metal-based furanyl derived sulfonamides. | a new series of antibacterial and antifungal furanyl-derived sulfonamides and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) metal complexes have been synthesized, characterized and screened for their in-vitro antibacterial activity against four gram-negative (escherichia coli, shigella flexneri, pseudomonas aeruginosa and salmonella typhi) and two gram-positive (bacillus subtilis and staphylococcus aureus) bacterial strains and, for in-vitro antifungal activity against trichophyton l ... | 2006 | 17252952 |
| outbreak of keratomycosis attributable to fusarium solani in the french west indies. | we report an unusual increase in the number of cases of keratomycosis attributable to fusarium solani in contact lens wearers. | 2007 | 17258535 |
| molecular characterization, biofilm analysis and experimental biofouling study of fusarium isolates from recent cases of fungal keratitis in new york state. | to characterize fusarium isolates from recent cases of fungal keratitis in contact lens wearers, and to investigate fungal association with moistureloc solution. | 2007 | 17263885 |
| cloning and sequence analysis of ornithine decarboxylase gene fragments from the ascomycota. | ornithine decarboxylase (odc; ec 4.1.1.17) catalyzes the initial step in the biosynthesis of polyamines, the conversion of ornithine to putrescine. based on the most conserved regions of fungal odcs, we designed and synthesized oligonucleotides to amplify homologous fragments of three important plant pathogenic pyrenomycete fungi (ascomycota), magnaporthe grisea, colletotrichum lindemuthianum and fusarium solani, and one insect pathogenic fungus metarhizium anisopliae. cloning and sequencing of ... | 2006 | 17286052 |
| in vitro and in vivo antifungal activity of cetrimide (cetyltrimethyl ammonium bromide) against fungal keratitis caused by fusarium solani. | mycotic keratitis is a devastating eye infection acquired after eye injury. cetrimide at 15 and 20 mg ml(-1) produced no surviving fusarium solani growth with minimal inhibitory concentration value of 0.10 mg ml(-1). topical administration of three drops (0.3 ml) of cetrimide aqueous solution of 10 mg ml(-1) at ph 6.4 three times daily succeeded to cure human severe resistant f. solani keratitis in a time course of <3 weeks, and with complete healing after 6 weeks. cetrimide-treated rabbit corne ... | 2007 | 17302751 |
| tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers. | cutinase from fusarium solani pisi was genetically modified near the active site, by site-directed mutagenesis, to enhance its activity towards polyethylene terephthalate (pet) and polyamide 6,6 (pa 6,6) fibers. the mutations l81a, n84a, l182a, v184a and l189a were done to enlarge the active site in order to better fit a larger polymer chain. modeling studies have shown enhanced free energy stabilization of model substrate tetrahedral intermediate (ti) bound at the enzyme active site for all mut ... | 2007 | 17306400 |
| chemical, thermal and ph-induced equilibrium unfolding studies of fusarium solani lectin. | the effect of urea, guanidine thiocyanate, temperature and ph was studied on the conformational stability of fusarium solani lectin. equilibrium unfolding with chemical denaturants showed that the lectin was least stable at ph 12 and maximally stable at ph 8.0 near its pi (8.7). guanidine thiocyanate (the concentration of denaturant at which the protein is half folded, d1/2 = 0.49 m at ph 12) was found to be an eight times stronger denaturant than urea (d1/2 = 3.88 m at ph 12). the unfolding cur ... | 2007 | 17365178 |
| [bilateral proximal cellulitis and onychomycosis in both big toes due to fusarium solani]. | we report a case of proximal fold cellulitis in both big toes, associated with a bilateral proximal onychomycosis and an intertrigo of the fourth space due to fusarium solani. the infection occurred in an immunocompetent man with diabetes mellitus type ii. apparently, the infection was acquired in a tropical country and once the patient was in spain the infection progressed causing nail detachment (onychomadesis). seven months later a relapse that affected the left toenail occurred. the patient ... | 2006 | 17388651 |
| chemical composition and antifungal activity of the essential oils of three species of artemisia on some soil-borne phytopathogens. | various species of the genus artemisia are used for their pharmacological, antimicrobial, antioxidant activity. three species of this genus, artemisia scoparia, a. sieberi and a. aucheri are widely distributed in desert area of iran. in order to identify the chemical composition, aerial parts of a. scoparia, a. sieberi and a. aucheri were collected from bajestan (khorasan province) at flowering stage. the essential oils were obtained by hydro-distillation of air-dried samples and their chemical ... | 2006 | 17390897 |
| modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents. | a comprehensive study of the hydration mechanism of an enzyme in nonaqueous media was done using molecular dynamics simulations in five organic solvents with different polarities, namely, hexane, 3-pentanone, diisopropyl ether, ethanol, and acetonitrile. in these solvents, the serine protease cutinase from fusarium solani pisi was increasingly hydrated with 12 different hydration levels ranging from 5% to 100% (w/w) (weight of water/weight of protein). the ability of organic solvents to 'strip o ... | 2007 | 17419728 |