Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| enhanced production of insecticidal proteins in bacillus thuringiensis strains carrying an additional crystal protein gene in their chromosomes. | a two-step procedure was used to place a cryic crystal protein gene from bacillus thuringiensis subsp. aizawai into the chromosomes of two b. thuringiensis subsp. kurstaki strains containing multiple crystal protein genes. the b. thuringiensis aizawai cryic gene, which encodes an insecticidal protein highly specific to spodoptera exigua (beet armyworm), has not been found in any b. thuringiensis subsp. kurstaki strains. the cryic gene was cloned into an integration vector which contained a b. th ... | 1995 | 7487039 |
| occurrence of fusarium species and zearalenone in dairy cattle feeds in vojvodina. | the occurrence of fusarium spp. and zearalenone (f-2 toxin) in dairy cattle feeds was studied during a period of three years (in all seasons) in vojvodina. fusarium species were found to be very common in feeds. they were classified into 11 different species (f. chlamydosporum, f. equiseti, f. flocciferum, f. graminearum, f. lateritium, f. moniliforme, f. oxysporum, f. semitectum, f. solani, f. tabacinum and f. tricinctum). some of these species are known to produce mycotoxins. the highest distr ... | 1995 | 7491864 |
| beeturia and colonic oxalic acid. | beeturia is the excretion of red beetroot pigment (betalaine) in urine and faeces. it occurs in about 14% of humans. betalaine is a redox indicator whose colour is protected by reducing agents. we investigated pigment-decolourizing systems in the intestinal tracts of beeturic and non-beeturic subjects. betalaine was decolourized by hydrochloric acid, ferric ions and colonic bacteria preparations, but not by pancreatic or mucosal enzymes. in animals, oral betalaine did not produce beeturia, but i ... | 1995 | 7493168 |
| location, size, and complexity of epitopes on the coat protein of beet necrotic yellow vein virus studied by means of synthetic overlapping peptides. | five regions on the coat protein of bnyvv which had been shown previously to be involved in the formation of continuous epitopes were further analyzed by means of synthetic overlapping peptides. it was found that at least some of these regions may encompass several overlapping epitopes (or parts thereof). four monoclonal antibodies (mabs) which were known to be specific for the c-terminus of bnyvv coat protein (amino acids 182-188 = rtsppgq) were found to react with different sets of peptides wh ... | 1994 | 7505070 |
| expression of the beet yellows closterovirus capsid protein and p24, a capsid protein homologue, in vitro and in vivo. | the positive-sense rna genome of beet yellows closterovirus (byv) encompasses open reading frames (orfs) for the viral capsid protein (cp, orf 6) and for a cp homologue (p24, orf 5). the sequences of the orfs 5 and 6 were inserted into an escherichia coli expression vector, pqe-9, under the control of the bacteriophage t5 promoter. the proteins were expressed in bacteria, purified, and used for antiserum production in rabbits. the recombinant byv cp and p24 showed serological cross-reactions whe ... | 1994 | 7515940 |
| vacuolar chloride regulation of an anion-selective tonoplast channel. | fluctuations in intravacuolar chloride concentrations affected the tonoplast inward (anion flux into the vacuole) currents of sugar beet (beta vulgaris). rising vacuolar chloride concentrations induced increases in the levels of nitrate, acetate and phosphate inward currents. these currents, evoked at physiological vacuolar potentials, showed a linear relationship with the concentration of vacuolar chloride between 6 and 100 mm. single channel currents revealed that rises in vacuolar chloride in ... | 1994 | 7519678 |
| antigenic structure of the coat protein of potato mop-top furovirus. | the antigenic structure of the coat protein (cp) of potato mop-top furovirus (pmtv) was studied by electron microscopy of virus particles labeled with gold-conjugated monoclonal antibodies (mabs) and by the reactions of mabs with overlapping octapeptides (pepscan) representing the complete amino acid sequence of the cp. a total of seven epitopes were identified in the cp. mab scr 69 detected a continuous epitope, which was located at the extreme n-terminus of the cp, was exposed at the surface a ... | 1994 | 7519806 |
| comparison of utilization of pectins from various sources by pure cultures of pectinolytic rumen bacteria and mixed cultures of rumen microorganisms. | utilization of citrus, lucerne, apple and sugar beet pulp pectins by pure strains of rumen bacteria, prevotella ruminicola, lachnospira multiparus and butyrivibrio fibrisolvens was compared. additionally, the utilization of pectins by mixed rumen microorganisms was evaluated. the comparison was based on the depletion of galacturonic acid from medium, content of cellular protein in the cultures and the amount of end products of pectin fermentation in cell-free culture fluids. it was found that ci ... | 1994 | 7526615 |
| determination of digestible energy values and fermentabilities of dietary fibre supplements: a european interlaboratory study in vivo. | the performance of methods to determine energy conversion factors for dietary fibre (df) supplements and fermentability (d) values of their non-starch polysaccharides (nsp) was investigated. heats of combustion, digestible energy (de) and d values were determined on five df supplements in five european laboratories on five separate occasions. in each instance the df supplements were fed to juvenile male wistar rats at two doses, 50 and 100 g/kg basal diet, for 3 weeks with food and faeces collec ... | 1995 | 7547845 |
| construction and expression of 75kda readthrough protein gene from beet necrotic yellow vein virus. | using dna recombinant techniques, coat protein (cp) gene and 54kda fragment from beet necrotic yellow vein virus (bnyvv) rna2 were ligated to construct 75kda readthrough protein gene. comparing with wild-type 75kda readthrough protein gene, four nucleotides of the constructed gene were replaced, including cp amber termination codon uag to aug. correspondingly, two amino acids were changed. the temperature inducible expression vectors containing 75kda readthrough protein gene or its 54kda fragmen ... | 1995 | 7548774 |
| analysis and chromosomal localization of retrotransposons in sugar beet (beta vulgaris l.): lines and ty1-copia-like elements as major components of the genome. | dna sequences of the reverse transcriptase gene of long terminal repeat (ltr) and non-ltr (non-viral) retrotransposons have been isolated and cloned from the genome of sugar beet (beta vulgaris). both retrotransposon types are highly amplified in sugar beet and may account for 2-5% of the genome. the bnr1 family, representing the first non-viral retrotransposon reported from a dicotyledonous species, shows homology to the mammalian l1 family of long interspersed repeated sequences (lines) and to ... | 1995 | 7551548 |
| detection of beet necrotic yellow vein virus using reverse transcription and polymerase chain reaction. | a diagnostic test based on reverse transcription followed by the polymerase chain reaction (rt-pcr) was developed for the detection of beet necrotic yellow vein virus (bnyvv). a specific 500-base-pair fragment was amplified from the read-through region of the coat protein gene located on rna-2. the viral origin of the amplified product was confirmed by sequencing, with the sequence obtained having 94.5% homology with published sequence data for bnyvv. the assay gave a sensitivity of 800 times th ... | 1995 | 7559854 |
| [dietary carotene intake and lung cancer among men from santiago]. | aiming to assess the association between carotenes and vitamin a intake and lung cancer, a case control study was performed. sixty one male subjects with lung cancer and 61 controls paired for age, sex and smoking habits from 6 hospitals in santiago were analyzed. based on a consumption tendency enquiry, the mean weekly intake of food groups, carotenes and vitamin a was calculated, considering seasonal variations. results showed that cases consumed less "winter" vegetables than controls (chard, ... | 1995 | 7569446 |
| [composition, physico-chemical properties and molecular superstructure of dietary fiber preparations of the cellan type]. | dietary fiber preparations of "cellan" type were prepared from apples, white cabbage, sugar beet pulp, soy hulls and wheat bran by treatment with amylolytic and proteolytic enzymes as well as by chemical extractions. scanning electron microscopic examinations show different morphological structures of the preparations and a high maintenance of native biomolecular superstructure. the content of pectin, protein, polysaccharide-hexoses and -pentoses and the composition of monosaccharides (also afte ... | 1995 | 7571866 |
| cloning, sequence and expression of the gene coding for rhamnogalacturonase of aspergillus aculeatus; a novel pectinolytic enzyme. | rhamnogalacturonase was purified from culture filtrate of aspergillus aculeatus after growth in medium with sugar-beet pulp as carbon source. purified protein was used to raise antibodies in mice and with the antiserum obtained a gene coding for rhamnogalacturonase (rhga) was isolated from a lambda cdna expression library. the cloned rhga gene has an open-reading frame of 1320 base pairs encoding a protein of 440 amino acids with a predicted molecular mass of 45 962 da. the protein contains a po ... | 1995 | 7576553 |
| pcr and non-isotopic labeling techniques for plant virus detection. | pcr technology permits the detection of viruses at levels several orders of magnitude lower than is possible by other methods. this high sensitivity facilitates detection of virus sequences during the early stages of infection of plants and in soil and vector samples. early detection of beet necrotic yellow vein virus (bnyvv) in beta vulgaris is an important part of the strategy for prevention of the spread of rhizomania, a commercially significant disease of sugar beet. a diagnostic test for bn ... | 1995 | 7580844 |
| voltage-gated ca2+ release channels in vacuolar membranes from beet storage roots and guard cells. | the ubiquity and wide-ranging roles of ca(2+)-mediated stimulus-response coupling in plant cells is likely to demand flexibility in the duration and amplitude of the cytosolic ca2+ signal. the presence of both ligand- and voltage-gated ca2+ release channels at the vacuolar membrane suggests that this flexibility might be achieved through differential regulation of these discrete channel classes. the properties and distribution of voltage-gated vacuolar ca2+ channels is described in this chapter. ... | 1994 | 7597637 |
| the plant vacuolar na+/h+ antiport. | salt stress imposes severe limitations on plant growth, however, the extent of growth reduction depends upon the soil salinity level and the plant species. one of the mechanisms employed by salt tolerant plants is the effective vacuolar compartmentalization of sodium. the sequestration of sodium into the vacuole occurs by the operation of a na+/h+ antiport located at the tonoplast. evidence for a plant vacuolar na+/h+ antiport has been demonstrated in tissues, intact vacuoles and isolated tonopl ... | 1994 | 7597639 |
| nucleotide sequence of beet mild yellowing virus rna. | the complete nucleotide sequence of the genomic rna of beet mild yellowing virus, isolate 2itb, is reported. the rna consists of 5722 nucleotides and contains six long open reading frames which conform to the arrangement characteristic of subgroup 2 luteoviruses. the three 3'-proximal open reading frames, which encode the viral coat protein, a putative movement protein and the readthrough domain, are highly homologous to the corresponding genes of beet western yellows luteovirus while the three ... | 1995 | 7611881 |
| isolation of cryptococcus neoformans var. neoformans from bird droppings, fruits and vegetables in mexico city. | the presence of cryptococcus neoformans in various natural sources, such as bird droppings, fruits and vegetables, was investigated. a total of 711 samples were analyzed; c. neoformans var. neoformans was isolated from seven out of 74 bird droppings (9.5%), with parrots as one of the most significant sources. fruits were positive in 9.5% of the 169 samples studied, specially citrus fruits, particularly grapefruit, in which the highest frequency was found. from the 468 vegetable samples, only 20 ... | 1995 | 7617014 |
| analysis of structure of sugar-beet pectin by enzymatic methods. | the structure of alkali-soluble pectin (asp) prepared from sugar-beet pulp was determined. asp was sequentially degraded by pure galactanase, rhamnogalacturonase and arabinosidase and sugar compositions and nmr analysis of the products used to assign the following tentative structure to asp: a backbone based on units of -->4)-alpha-d-galpa-(1--> 2)-alpha-l-rhap-(1-->, and side chains both of arabinan and of arabinogalactan. | 1995 | 7626265 |
| chronic and postprandial responses of plasma insulin, glucose and lipids in volunteers given dietary fibre supplements. | we questioned whether a dietary fibre supplement known to lower fasting plasma cholesterol concentrations can also lower the postprandial plasma cholesterol, glucose and insulin concentrations when it is administered just before a meal. two studies were conducted in healthy middle-aged volunteers of both sexes in whom the fasting plasma total cholesterol concentrations were above normal. in the first study the dietary fibre treatments (2.2 g) were psyllium and a psyllium-citrus pectin mixture to ... | 1995 | 7626592 |
| dietary fiber for dogs: iv. in vitro fermentation of selected fiber sources by dog fecal inoculum and in vivo digestion and metabolism of fiber-supplemented diets. | two experiments were conducted to evaluate single sources and blends of dietary fiber in dog food. in exp. 1, 14 fibrous substrates were fermented in vitro using dog feces as the source of inoculum. organic matter disappearance was lowest (p < .05; < 10%) for solka floc and oat fiber and greatest (p < .05; > 80%) for fructooligosaccharides (fos) and lactulose. solka floc, oat fiber, gum karaya, and xanthan gum produced the least (p < .05; < 1 mmol/g of substrate om) total short-chain fatty acids ... | 1995 | 7628954 |
| in vitro fermentation of selected fibrous substrates by dog and cat fecal inoculum: influence of diet composition on substrate organic matter disappearance and short-chain fatty acid production. | two in vitro fermentation experiments were conducted to evaluate the influence of source of dietary fiber fed to dogs and cats on fermentative activity of their fecal microflora. in exp. 1, six english pointer dogs were fed a diet containing either a non-fermentable fiber (solka floc) or a fermentable fiber (citrus pulp). a fecal sample from each dog was used as the inoculum source to determine in vitro om disappearance (omd) and short-chain fatty acid (scfa) production from selected fibrous sub ... | 1995 | 7628955 |
| the consequences of fruit and vegetable fibre fermentation on their binding capacity for meiqx and the effects of soluble fibre sources on the binding affinity of wheat bran preparations. | fruits and vegetables provide dietary fibre some of which is partly soluble in the upper gut; in the colon it is highly fermentable. using alcohol-insoluble residues prepared from a range of fruits and vegetables the effects of fermentation on the changes in composition and binding capacity have been assessed for the hydrophobic mutagen 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx). fermentation was extensive and resulted in destruction of most of the pectic polysaccharides. of the unfer ... | 1995 | 7634394 |
| detection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture rt-pcr products. | single-strand conformation polymorphism analysis was found to be a powerful tool for rapidly assigning large numbers of beet necrotic yellow vein virus (bnyvv) isolates to a known strain group as well as for detecting mixed infections, minor variants or new strain groups. the prevalence of the b-type in germany and france and the a-type in most other countries was confirmed. minor variants with a very restricted distribution were detected occasionally. new rhizomania outbreaks in great britain w ... | 1995 | 7636486 |
| atp, ph and mg2+ modulate a cation current in beta vulgaris vacuoles: a possible shunt conductance for the vacuolar h(+)-atpase. | macroscopic instantaneous and time-dependent currents have been measured in the vacuolar membrane of beta vulgaris using a patch clamp configuration analogous to whole cell mode. at low cytosolic ca2+ and in the absence of mg2+, only an instantaneous current was observed. this current is carried predominantly by cations (pk:pcl 7:1, pna:pcl 4:1 and arginine is also conducted). the instantaneous current can be activated by atp4- (e.g., atp-activated mean k+ current density was -20 ma.m-2 at a mem ... | 1995 | 7636887 |
| construction of a rhizosphere pseudomonad with potential to degrade polychlorinated biphenyls and detection of bph gene expression in the rhizosphere. | the genetically engineered transposon tnpcb, contains genes (bph) encoding the biphenyl degradative pathway. tnpcb was stably inserted into the chromosome of two different rhizosphere pseudomonads. one genetically modified strain, pseudomonas fluorescens f113pcb, was characterized in detail and found to be unaltered in important parameters such as growth rate and production of secondary metabolites. the expression of the heterologous bph genes in f113pcb was confirmed by the ability of the genet ... | 1995 | 7646029 |
| characterization and localization of new antifungal cysteine-rich proteins from beta vulgaris. | two novel antifungal proteins, ax1 and ax2, were isolated from leaves of sugar beet infected with cercospora beticola. ax1 (mw = 5078 +/- 3d) and ax2 (mw = 5193 +/- 3d) were n-terminally sequenced and identified as monomeric, basic proteins consisting of 46 amino acid residues, of which eight are cysteines. both ax proteins strongly inhibit growth of c. beticola and other filamentous fungi, but have little or no effect against bacteria. based on primary sequence homology (24 to 46% identity), th ... | 1995 | 7655063 |
| early iron deficiency stress response in leaves of sugar beet. | iron nutrient deficiency was investigated in leaves of hydroponically grown sugar beets (beta vulgaris) to determine how ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) gene expression is affected when thylakoid components of photosynthesis are diminished. rubisco polypeptide content was reduced by 60% in severely iron-stressed leaves, and the reduction was directly correlated to chlorophyll content. the concentration of rubisco protein in iron-stressed leaves was found to be regulated ... | 1995 | 7659749 |
| kinetics of hydration and functional specific gravity of fibrous feed by-products. | five studies were conducted to evaluate the kinetics of digestion, hydration, and functional specific gravity (fsg) of various feed by-products (fbp) in vitro. the water-holding capacity (whc) of alfalfa and orchardgrass (1.428 and 1.005 g/g of insoluble dm [idm], respectively) was higher (p < .05) than the whc of fbp, which ranged from .175 for distillers grains to .481 g/g of idm for brewers grains pellets. rate of hydration was the highest in brewers grains pellets and beet pulp (.215 and .25 ... | 1995 | 7665376 |
| [food conversion in the digestive system of juvenile bulls. 1. conversion of protein and amino acids]. | fistulated bulls (duodenal bridge fistulas) received 10 rations containing 60% concentrate and combinations of carbohydrates from various sources to investigate the protein and amino acid flux in the digestive tract. with a constant crude protein concentration of 12-13% in the rations (adjusted by adding urea), an average of 85 +/- 8% of the crude protein ingested with the feed were found in the duodenum. the highest values were measured for rations containing wheat/dried sugar beet chips (97% a ... | 1994 | 7668969 |
| [food conversion in the digestive system of juvenile bulls. 3. rumen liquor investigations]. | two bulls with a permanent rumen fistula and a re-entrant cannula at the duodenum were fed various concentrates and straw (dry matter ration composition: 60% concentrate, 40% straw). negative correlations were found between the ph value and total fatty acid concentration (r = -0.70). the animals were given the straw one hour before the concentrate. the ph value increased to not more than 7.3 one hour after they had received the straw. it did not drop below 6.4 after the concentrate with any of t ... | 1994 | 7668978 |
| [dry matter and nutrient degradability of foodstuffs after incubation in the rumen (nylon bag technique)]. | the digestion of dry matter and nutrients of wheat/barley, maize, potatoes, dried sugar beet chips dried sugar beet pulp and barley straw were studied by means of the nylon bag method on two steers with fistulated rumens. compared with that of the other feedstuffs with a high starch content, the disappearance of maize was significantly lower in terms of dry matter (20%), starch (38%) and crude protein (23%). in the case of cereals, dry matter degradability correlated closely with the disappearan ... | 1994 | 7668979 |
| differential response to frameshift signals in eukaryotic and prokaryotic translational systems. | the genomic rna of beet western yellows virus (bwyv) contains a potential translational frameshift signal in the overlap region of open reading frames orf2 and orf3. the signal, composed of a heptanucleotide slippery sequence and a downstream pseudoknot, is similar in appearance to those identified in retroviral rnas. we have examined whether the proposed bwyv signal functions in frameshifting in three translational systems, i.c. in vitro in a reticulocyte lysate or a wheat germ extract and in v ... | 1993 | 7680118 |
| transcriptional analysis of the virion-sense genes of the geminivirus beet curly top virus. | the genome of the geminivirus beet curly top virus (bctv) consists of a single circular dna containing overlapping open reading frames (orfs) located on both the virion-sense and complementary-sense dna strands. to investigate the expression of these orfs, rna extracted from infected nicotiana benthamiana and beta vulgaris has been examined for the presence of viral transcripts. an abundant 1.1-kb virion-sense polyadenylated rna and four complementary-sense polyadenylated rnas of 1.7, 1.5, 1.3, ... | 1993 | 7692668 |
| "rattlesnake" structure of a filamentous plant rna virus built of two capsid proteins. | elongated particles of simple rna viruses of plants are composed of an rna molecule coated with numerous identical capsid protein subunits to form a regular helical structure, of which tobacco mosaic virus is the archetype. filamentous particles of the closterovirus beet yellow virus (byv) reportedly contain approximately 4000 identical 22-kda (p22) capsid protein subunits. the byv genome encodes a 24-kda protein (p24) that is structurally related to the p22. we searched for the p24 in byv parti ... | 1995 | 7708667 |
| papain-like proteinase of turnip yellow mosaic virus: a prototype of a new viral proteinase group. | sequence comparisons predicted a potential papain-like proteinase domain in the n-terminal cleavage product (nrp) of the large nonstructural replicase polyprotein (rp) of turnip yellow mosaic virus (tymv). replacement of the predicted catalytic amino acids, cys-783 by ser, or of his-869 by glu, abolished cleavage of the 206k rp into a approximately 150 k nrp and a approximately 78 k c-terminal product in reticulocyte lysates, while other substitutions exerted no apparent influence on proteolysis ... | 1995 | 7710355 |
| primary structure and sequence analysis of rna2 of a mechanically transmitted barley mild mosaic virus isolate: an evolutionary relationship between bymo- and furoviruses. | the rna2 nucleotide sequence of a mechanically transmitted isolate of barley mild mosaic virus (bammv) has been determined. a combination of northern blot and sequence analysis indicates that this rna2 lacks approximately 1000 nucleotides of its c-terminal protein (p2) gene, with respect to polymyxa graminis transmitted bammv. this is confirmed by sequence comparison with rna2 of a fungus transmitted bammv isolate, which reveals the presence of a single deletion located in the 3'-terminal part o ... | 1995 | 7710357 |
| [expression in bacteria and some properties of p65, a homolog of cellular heat shock proteins hsp70 coded in the rna of the beet yellows virus genome]. | 1995 | 7719251 | |
| plaque assay for spodoptera exigua and autographa californica nuclear polyhedrosis viruses in a newly established cell line of the beet armyworm, spodoptera exigua. | the nuclear polyhedrosis viruses of spodoptera exigua (senpv) and autographa californica (acnpv) produced plaques in a newly established cell line of the beet armyworm, spodoptera exigua. plaques were composed of infected cells containing many polyhedra and were visible without any staining procedure. dose-response assays showed a direct correlation between the number of plaques and the inoculum size. growth kinetic studies of the two viruses, using the developed plaque assay system, revealed th ... | 1994 | 7726004 |
| release of ca2+ from individual plant vacuoles by both insp3 and cyclic adp-ribose. | calcium mobilization from intracellular pools couples many stimuli to responses in plant cells. cyclic adenosine 5'-diphosphoribose (cadpr), which interacts with a ryanodine receptor in certain animal cells, was shown to elicit calcium release at the vacuolar membrane of beet storage root. the vacuolar calcium release pathway showed similarities to cadpr-gated calcium release in animal cells, including inhibition by ruthenium red, ryanodine activation, and high affinity for cadpr [michaelis cons ... | 1995 | 7732384 |
| beet fed as such or ensiled with maize and fresh potatoes in diets for finishing bulls. | the use of beet for beef production after ensiling together with maize was studied in two experiments involving 112 and 114 white-blue bulls. in the first experiment maize silage (i) was compared with mixed silage of maize and fodder beet (ii) or sugar beet (iii) (ratio: 2/1, dm-basis). in the second experiment maize silage (i) was compared with mixed silage of 0.67 maize and 0.33 sugar beet (ii) or maize silage and fresh sugar beet (ratio: 2/1, dm basis) (iii) or fresh sugar beet and potatoes a ... | 1994 | 7733816 |
| the chloroplast trnp-trnw-petg gene cluster in the mitochondrial genomes of beta vulgaris, b. trigyna and b. webbiana: evolutionary aspects. | the chloroplast trnp-trnw-petg gene cluster has been identified in the mitochondrial dna (mtdna) of sugar beet (beta vulgaris). the chloroplast-derived trnw gene is transcribed in the mitochondria; the other two genes, however, do not seem to be transcribed. this gene cluster is also present in the mitochondrial genomes of two wild beta species, b. trigyna and b. webbiana. sugar beet and the two wild relatives share 100% sequence identity in the coding regions of both the mitochondrial trnp and ... | 1995 | 7736615 |
| ruminal fermentation and digestion in lactating cows fed grass silage with protein and energy supplements. | four multiparous holstein cows were used in a 4 x 5 incomplete latin square design to study the effects of different dietary sources of energy and protein on digestion, ruminal fermentation, and degradability in cows fed high moisture grass silage. the five treatments were an all silage diet (control); silage and concentrate containing soybean meal fed with corn, beet pulp, or a mixture (50:50 on a dm basis) of oats and barley; and fish meal fed with beet pulp. concentrates were fed between .70 ... | 1995 | 7745154 |
| milk production and intake of lactating cows fed grass silage with protein and energy supplements. | primiparous (n = 8) and multiparous (n = 24) holstein cows averaging 591 kg of bw (se = 12) were allotted at 2 wk postpartum to eight blocks of 4 cows each on the basis of calving date and parity. cows were fed timothy silage for ad libitum intake. the experiment was carried out between wk 4 and 15 of lactation. cows within each block were assigned randomly to a concentrate containing soybean meal fed with corn, beet pulp, or a mixture of oats and barley (50: 50 on a dm basis) or fish meal fed w ... | 1995 | 7745155 |
| effect of substituting steam-flaked sorghum for concentrate on lactation and digestion in dairy cows. | thirty-five lactating cows (110 dim), allotted to five dietary treatments for 70 d, were fed 0, 15, 30, or 45% of dietary dm as steam-flaked sorghum grain (360 g/l) in place of equal amounts of a mixture (dm basis) of 40% steam-rolled corn, 13% rolled barley, 20% wheat mill run, and 27% ground beet pulp in four diets. thinly flaked sorghum grain (283 g/l) was also fed at 15% of dietary dm. the dmi tended to increase as flaked sorghum increased. yields of milk and milk protein and percentages of ... | 1995 | 7745156 |
| complete sequence of the citrus tristeza virus rna genome. | the sequence of the entire genome of citrus tristeza virus (ctv), florida isolate t36, was completed. the 19,296-nt ctv genome encodes 12 open reading frames (orfs) potentially coding for at least 17 protein products. the 5'-proximal orf 1a starts at nucleotide 108 and encodes a large polyprotein with calculated mw of 349 kda containing domains characteristic of (from 5' to 3') two papain-like proteases (p-pro), a methyltransferase (mt), and a helicase (hel). alignment of the putative p-pro sequ ... | 1995 | 7747424 |
| antibodies to the tonoplast from the storage parenchyma cells of beetroot recognize a major intrinsic protein related to tips. | a vacuole membrane (= tonoplast) subfraction was purified by sedimentation in a discontinuous sucrose gradient (0.6 m/0.3 m) from a crude vacuole fraction isolated from red beetroot storage cells. the vacuole membrane-enriched fraction was injected into the popliteal lymph nodes of rabbits to raise polyclonal antibodies. the resultant serum was tested by indirect immunofluorescence microscopy on cryosections of shoot meristem. antibodies specifically bound to the tonoplast but not the cytoplasm ... | 1995 | 7750515 |
| cloning of the aspergillus niger gene encoding alpha-l-arabinofuranosidase a. | using l-arabitol as an inducer, simple induction conditions were established that resulted in high-level expression of alpha-l-arabinofuranosidase a by an aspergillus niger d-xylulose kinase mutant strain. these conditions were adapted to construct a cdna expression library from which an alpha-l-arabinofuranosidase a cdna clone was isolated using specific antiserum. the corresponding gene encoding alpha-l-arabinofuranosidase a (abfa) was isolated from a genomic library and cloned into a high cop ... | 1993 | 7764056 |
| molecular cloning, expression and structure of the endo-1,5-alpha-l-arabinase gene of aspergillus niger. | secretion of endo-1,5-alpha-l-arabinase a (abn a) by an aspergillus niger xylulose kinase mutant upon mycelium transfer to medium containing l-arabitol was immunochemically followed with time to monitor its induction profile. a cdna expression library was made from polya+ rna isolated from the induced mycelium. this library was immunochemically screened and one abn a specific clone emerged. the corresponding abna gene was isolated from an a. niger genomic library. upon southern blot analysis, a ... | 1993 | 7764386 |
| metabolic engineering of glycine betaine synthesis: plant betaine aldehyde dehydrogenases lacking typical transit peptides are targeted to tobacco chloroplasts where they confer betaine aldehyde resistance. | certain higher plants synthesize and accumulate glycine betaine, a compound with osmoprotectant properties. biosynthesis of glycine betaine proceeds via the pathway choline-->betaine aldehyde-->glycine betaine. plants such as tobacco (nicotiana tabacum l.) which do not accumulate glycine betaine lack the enzymes catalyzing both reactions. as a step towards engineering glycine betaine accumulation into a non-accumulator, spinach and sugar beet complementary-dna sequences encoding the second enzym ... | 1994 | 7764986 |
| microbial production of propionic acid and vitamin b12 using molasses or sugar. | with a cell concentration of 125 g dry biomass l-1 and a dilution rate of 0.1 h-1, propionibacterium acidipropionici produces 30 g propionic acid l-1 from sugar with a productivity of 3 g l-1 h-1. the yield of propionic acid is approx. 0.36-0.45 g propionic acid g-1 sucrose and is independent of the dilution rate and cell concentration. acetic acid is an unwanted by-product in the production of propionic acid. the concentration of acetic acid only increases slightly when the cell concentration i ... | 1994 | 7765100 |
| phenolics induced in beta vulgaris by rhizoctonia solani infection. | rhizoctonia solani inoculated sugarbeet roots produced the new beta-xyloside and beta-glucoside of 2'-hydroxy-6,7-methylenedioxy-5- methoxyisoflavone (betavulgarin) in response to infection. also formed were 6,7-methylenedioxy-5-methoxyflavone, 6,7-methylenedioxy-5-methoxydihydroflavonol and 3'-methoxy-4',5,7-trihydroxy-dihydroflavonol, not reported previously in beta vulgaris. | 1994 | 7765684 |
| seco-glycosides of oleanolic acid from beta vulgaris. | two new oleanolic acid saponins were isolated from the leaves and roots of beta vulgaris. both contained the unusual feature of a 3,4 seco-glycopyranosyl moiety. their structures were established by a combination of 2d nmr experiments and of californium plasma desorption mass spectrometry. | 1994 | 7766003 |
| chemical modification and amino acid sequence of active site in sugar beet alpha-glucosidase. | the modification of amino acid residues in sugar beet alpha-glucosidase with conduritol b epoxide (cbe), an affinity labeling reagent, inactivated the enzyme. the inactivation followed pseudo-first-order kinetics. the enzyme was protected from inactivation by a competitive inhibitor, tris, and the partially inactivated enzymes showed only the decrease of v values and no change in km value. an 3h-cbe labeled peptide isolated from the digest of the inactivated enzyme with lys-c protease was sequen ... | 1995 | 7766184 |
| identification of potential redox-sensitive cysteines in cytosolic forms of fructosebisphosphatase and glyceraldehyde-3-phosphate dehydrogenase. | tertiary-structure modeling suggests the occurrence of disulfide bonds in the cytosolic form of fructose-bisphosphatase (ec 3.1.3.11) in spinach (spinacia oleracea l.), sugarbeet (beta vulgaris l.) and potato (solanum tuberosum l.). redox modulation could then control the amp sensitivity of fructosebisphosphatase in the cytosol, as suggested by the experiments of e. khayat et al. (1993, plant physiol. 101, 57-64). modeling also reveals two cysteine residues correctly positioned to form a disulfi ... | 1995 | 7767233 |
| cloning and expression analysis of sucrose-phosphate synthase from sugar beet (beta vulgaris l.). | a cdna clone encoding a sucrose-phosphate synthase from sugar beet (bvsps 1) has been isolated by screening a tap root-specific cdna library using a heterologous sps cdna from spinach. the 3635 bp sugar beet cdna codes for a 1045 amino acid polypeptide with a predicted molecular mass of 118 kda. the deduced amino acid sequence of sugar beet sps shows homologies with sps from maize (71% identity) and spinach (77% identity). genomic southern blot analysis suggests that bvsps 1 is a low-copy-number ... | 1995 | 7770061 |
| cloning of genes required for hypersensitivity and pathogenicity in pseudomonas syringae pv. aptata. | a genomic library of pseudomonas syringae pv. aptata strain ncppb 2664, which causes bacterial blight of sugar beet, lettuce and other plants, was constructed in the cosmid vector pcpp31. the 13.4 kb ecori fragment of the cosmid phir11, containing the hrp (hypersensitive response and pathogenicity) gene cluster of the closely related bacterium pseudomonas syringae pv. syringae strain 61, was used as a probe to identify a homologous hrp gene cluster in p. syringae pv. aptata. thirty of 2500 cosmi ... | 1995 | 7771767 |
| a novel protein from mung bean hypocotyl cell walls with acetyl esterase activity. | an acetyl esterase was purified from cell walls isolated from mung bean hypocotyls. the purified enzyme had an apparent mr of 43,300 and an apparent pi > 9. it rapidly deesterified triacetin and p-nitrophenylacetate and slowly released acetate from beet and flax pectins, the deesterification rate being increased by previous demethylation of the pectins. no significant peptide sequence identity between the acetyl esterase and any known protein could be found in protein data bases. | 1995 | 7772302 |
| purification and properties of 6-phosphogluconate dehydrogenase from beet leaves. | we have purified to homogeneity 6-phosphogluconate dehydrogenase from leaves of silver beet (beta vulgaris l.) by means of cation-exchange and affinity chromatography. the enzyme is a homodimer of 52 kda subunits; it catalyzes nadp dependent oxidation of 6-p-gluconate with michaelian substrate saturation. the activity is affected by some intermediates of carbohydrate metabolism, particularly erythrose-4-p. subcellular fractionation studies indicate the cytosolic location of the enzyme. | 1995 | 7773202 |
| the voltage-gated ca2+ release channel in the vacuolar membrane of sugar beet resides in two activity states. | the voltage-gated ca2+ release channel in the vacuolar membrane of sugar beet tap roots resides in two states which show similar reversal potentials in biionic conditions but differ dramatically in their single channel current and open frequency. state i has a unitary ca2+ conductance of 3.9 +/- 0.5 ps and channel openings are rare at vacuolar resting potentials. after spontaneous and reversible transition into state ii the unitary ca2+ conductance increases three-fold, accompanied by a concomit ... | 1995 | 7774705 |
| [the content of nitrates and nitrites in fruits, vegetables and other foodstuffs]. | there are presented the results of the investigation of many popular fresh and frozen vegetables as well as in fresh and frozen fruits, such as different kind of apples, strawberries, currants, raspberries and many other tender fruits mainly from market and few from own allotment-cottages performed in the year 1989-1992. the nitrate and nitrite was determined according to the griess reaction. high levels of nitrate was encountered in lettuce, frozen spinach, fennel, radishes, parsley. many of th ... | 1994 | 7777773 |
| plasma membrane intrinsic proteins of beta vulgaris l. | the plasma membrane (pm) of higher plants contains numerous proteins; however, due to their low abundance, only a few have been identified and characterized by direct biochemical approaches. the major intrinsic protein (mip) family is a class of highly hydrophobic integral membrane proteins thought to function as channels that facilitate the passage of water, small solutes, and possibly other moieties through the membrane. a family of pm intrinsic proteins was purified and characterized from pm ... | 1995 | 7784509 |
| quantity and economic importance of nine selected by-products used in california dairy rations. | food processing representatives, brokers, nutritionists, livestock producers, and trade associations were surveyed to quantify 9 by-products used for feeding livestock during 1992 in california. the commodities were almond hulls, dried beet pulp, wet brewers grains, wet citrus pulp, pressed citrus pulp, wet corn gluten feed, corn gluten meal, whole cottonseed, and rice bran. the 9 by-products contributed over 2.5 million tonnes and about 27% of the total feed concentrate moved within california ... | 1995 | 7790589 |
| [accumulation of heavy metals (pb, cd) in selected varieties of vegetables]. | vegetables have a great share in supplying of lead and cadmium with food into the human organism. for this reason an attempt was undertaken for quantitative evaluation of accumulation of those metals in three varieties of the following vegetables: carrots, parsley, garden beet, cabbage and potato grown at identical fertilization, weather conditions and pollution. cadmium and lead were complexed with diethyldithiocarbamate, extracted into organic phase, and determined using atomic absorption spec ... | 1994 | 7792516 |
| the small cysteine-rich protein p14 of beet necrotic yellow vein virus regulates accumulation of rna 2 in cis and coat protein in trans. | the effect of null mutations of the small cysteine-rich protein p14 encoded by rna 2 of beet necrotic yellow vein virus has been investigated using in vitro transcripts of viral rna to infect chenopodium quinoa protoplasts. the p14 mutations down-regulated rna 2 accumulation by approximately 10- to 50-fold. accumulation of minus-strand rna 2 was also diminished but rna 1 accumulation was much less affected. the inhibition of rna 2 accumulation could not be complemented in trans by providing p14 ... | 1995 | 7793082 |
| transition state structures for the hydrolysis of alpha-d-glucopyranosyl fluoride by retaining and inverting reactions of glycosylases. | secondary tritium and primary 14c kinetic isotope effects were measured for the hydrolysis of alpha-d-glucopyranosyl fluoride catalyzed by sugar beet seed alpha-d-glucosidase, forming alpha-d-glucose, and by rhizopus niveus glucoamylase forming beta-d-glucose. the data provided a novel opportunity to model and directly compare the transition state structures for the hydrolysis of a substrate promoted with retention or inversion of configuration according to the enzyme catalyst. the isotope effec ... | 1994 | 7798231 |
| isolation and purification of feruloylated oligosaccharides from cell walls of sugar-beet pulp. | cell walls from sugar-beet pulp contain some feruloyl groups linked to the pectic neutral side-chains. enzymic as well as chemical hydrolysis of the pulp yielded a series of feruloylated oligosaccharides, which have been purified by sephadex lh-20 and biogel p-2 chromatography in aqueous solvents. feruloylated arabinose di-, tri-, hexa-, hepta-, and octa-saccharides as well as feruloylated galactose disaccharides were obtained after hydrolysis of the pulp with a mixture of fungal carbohydrases ( ... | 1994 | 7805051 |
| structure identification of feruloylated oligosaccharides from sugar-beet pulp by nmr spectroscopy. | 1d nmr (1h and 13c) and 2d nmr spectroscopy have been used to determine the structure of feruloylated oligosaccharides obtained by enzymic degradation or mild acid hydrolysis of sugar-beet pulp. feruloylated oligosaccharides derived from pectic neutral side-chains containing arabinose or galactose residues were identified. in the feruloylated arabinose oligosaccharides, feruloyl groups were linked to o-2 of l-ara f residues. the structure of the feruloylated arabinose disaccharide was identified ... | 1994 | 7805052 |
| degradation of feruloylated oligosaccharides from sugar-beet pulp and wheat bran by ferulic acid esterases from aspergillus niger. | the activity of two forms of ferulic acid esterase (fae) from aspergillus niger on a synthetic feruloylated substrate (methyl ferulate) and on 11 different feruloylated oligosaccharides from sugar-beet pulp and wheat bran was determined. the enzymes exhibited different specificities for the various feruloylated substrates and were more active on certain substrates of cell-wall origin than on methyl ferulate. both enzymes preferred the arabinose residue to which ferulic acid is attached in the fu ... | 1994 | 7805053 |
| apolipoprotein mrna in liver and intestine of rats is affected by dietary beet fiber or cholestyramine. | rats were fed a cholesterol-free diet with no added fiber (fiber-free) or with 15 g/100 g beet fiber or 5 g/100 g cholestyramine for 14 d. final plasma total cholesterol concentrations were significantly lower in rats fed beet fiber than in those fed fiber-free or cholestyramine diets. this difference was due mainly to lower hdl cholesterol concentrations. the group fed beet fiber also tended (p < 0.1) to have lower apolipoprotein a-i concentration in plasma. northern blot analysis revealed that ... | 1995 | 7815170 |
| sequence analysis and gene content of potato mop-top virus rna 3: further evidence of heterogeneity in the genome organization of furoviruses. | the complete sequence of the 2315 nucleotides in rna 3 of potato mop-top furovirus (pmtv) isolate t was obtained by analysis of cdna clones and by direct rna sequencing. the sequence contains an open reading frame for the coat protein (20k) terminated by an amber codon, followed by an in-phase coding region for an additional 47k. pmtv therefore resembles soil-borne wheat mosaic (sbwmv) and beet necrotic yellow vein (bnyvv) viruses (two other fungus-transmitted viruses with rod-shaped particles) ... | 1995 | 7831829 |
| effects of concentrated separator dried beet pulp on nutrient digestibility, milk production, and preference of holstein cattle. | concentrated separator by-product was fed to cattle to determine tdn, milk production, and feed preference. the tdn content of concentrated separator by-product was determined using a substitution method involving a basal diet of alfalfa and grass hay and a treatment diet of alfalfa and grass hay plus 10% added concentrated separator by-product. four ruminally cannulated cows in a replicated 2 x 2 latin square design were used. the estimated tdn content of concentrated separator by-product was 5 ... | 1994 | 7836593 |
| classification and identification of geminiviruses using sequence comparisons. | the genomes and orfs of 36 geminiviruses were compared to obtain phylogenetic trees and frequency distributions of all possible pairwise comparisons with an objective to classify geminiviruses. such comparisons show that geminiviruses form two distinct clusters of leafhopper-transmitted viruses that infect monocots (subgroup i) and whitefly-transmitted viruses that infect dicots (subgroup iii), irrespective of the part of the genome considered. of the two leafhopper-transmitted viruses that infe ... | 1995 | 7844548 |
| genetic analysis of beet curly top virus: examination of the roles of l2 and l3 genes in viral pathogenesis. | the monopartite dna genome of beet curly top geminivirus (bctv, strain logan) contains four leftward (complementary sense) open reading frames (orfs) designated l1, l2, l3, and l4. we investigated the functions of the l2 and l3 orfs by mutational analysis. we found that in nicotiana benthamiana and sugarbeet plants, neither a functional l2 nor a functional l3 gene is required for infectivity. double mutants were also infectious, and no evidence for a synergistic effect of these genes was evident ... | 1995 | 7856079 |
| a recombinational event in the history of luteoviruses probably induced by base-pairing between the genomes of two distinct viruses. | alignments of luteovirus readthrough protein amino acid sequences show they consist of two distinct regions, here named the n domain and the c domain. n domain sequences were classified, and comparison of this gene phylogeny to phylogenies of other luteovirus genes revealed an anomaly in the relationships between beet western yellows luteovirus, cucurbit aphidborne yellows luteovirus (cabyv), and pea enation mosaic rna1 (pemv1). together with alignments of virion protein and readthrough protein ... | 1995 | 7856086 |
| strain-specific determinants of beet curly top geminivirus dna replication. | the logan and cfh strains of the geminivirus beet curly top virus (bctv) possess cis- and trans-dna replication factors which exhibit specificity and are not functionally interchangeable. we demonstrate that the cis-acting replication specificity element is entirely contained within a 82- to 97-bp fragment which includes most of the viral dna origin of replication. we also demonstrate that the strain-specific trans-acting replication determinant is located within amino acid residues 3-89 of the ... | 1995 | 7856103 |
| [beet necrotic yellow vein virus transmitted by the fungus polymyxa betae]. | 1994 | 7856119 | |
| isolation and expression analysis of cdna clones encoding a small and a large subunit of adp-glucose pyrophosphorylase from sugar beet. | the cdna cloning of a small and a large subunit of adp-glucose pyrophosphorylase (agpase) from sugar beet is reported. the deduced amino acid sequences are highly homologous to previously identified agpase polypeptides from other plant species. both subunits are encoded by low copy genes. when rna gel blot experiments were performed, strongest expression was detected in sink and source leaves of greenhouse-grown sugar beet plants. a lower expression was found in other tissues tested, i.e. in the ... | 1995 | 7865789 |
| a proline-rich chitinase from beta vulgaris. | a gene (ch1) encoding a novel type of chitinase was isolated from beta vulgaris. the ch1 protein consists of an n-terminal hydrophobic prepeptide of 25 amino acids followed by a hevein-like domain of 22 amino acid residues, an unusually long proline-rich domain of 131 amino acid residues with 90 prolines, and finally a catalytic domain of 261 amino acid residues. proteins with similar proline-rich domains are present in some other plants. the ch1 gene shows a transient expression in response to ... | 1995 | 7865792 |
| [effects of reduced food intake on digestive flux and use of nitrogenous matter by goats]. | limited feeding effects (75% of ad libitum) were studied in 3 adult dry goats. their diet was composed of dried beet pulps (1/3 dm), dried alfalfa (1/3 dm) and hay (1/3 dm). the cp content was 11.9% dm. rumen fermented om was decreased by 243 g.d-1, but rumen ph and ammonia content remained the same. rumen bacteria synthesis efficiency was decreased (-4%), but the value (151 g crude bacteria protein.kg-1 dom) was higher than conventional values used in pdi and nrc systems. nitrogen rumen balance ... | 1995 | 7873041 |
| [value of harmful elements in fruit and vegetables grown in the province of siedlce]. | the content of iron, manganese, nickel, lead, copper, zinc and cadmium was determined by means of atomic absorption spectrometry (asa) in vegetables and fruit grown in the province of siedlce. the studied fruits included tomatoes, cucumbers, cabbages, carrots, parsley, celery, red beets, potatoes garden strawberries, black and red currants, raspberries, apples, plums and cherries. the total number of studied samples of fruits and vegetables was 229. in 49 studied samples cadmium exceeded 0.03 mg ... | 1994 | 7878342 |
| [content of nitrates and nitrites in early vegetables and potatoes sold in the marketplace of białystok in the year 1992]. | nitrate and nitrite were determined in early vegetables and potatoes sold in białystok in 1992. the levels of nitrates and nitrites were determined in 248 samples. nitrate and nitrite were evaluated spectrophotometrically by the method of the griess reaction, after reduction of the nitrates to nitrites, with cadmium dust. high values of nitrate were found in the early vegetables: lettuce, young red beets, radishes, dills, parsley, carrots, cucumbers and potatoes. the results were higher than the ... | 1994 | 7878349 |
| [value of nitrates and nitrites in selected vegetables and potatoes sold in the marketplace of białystok in the years 1991-1992]. | nitrate and nitrite contents were determined in selected vegetables and potatoes from the market in białystok and from ecology cultivation in the years 1991-1992. the modified griess method was used for determination of nitrate. the results showed that the nitrate contents in red beets, cabbages, parsley, carrots, celery and potatoes, were higher than the suggested limit in poland. in the group of vegetables from special cultivation plots, the nitrate contents also exceeded the maximal permissib ... | 1994 | 7878350 |
| aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein p74. | beet western yellows luteovirus is obligately transmitted by the aphid myzus persicae in a circulative, non-propagative fashion. virus movement across the epithelial cells of the digestive tube into the hemocoel and from the hemocoel into the accessory salivary glands is believed to occur by receptor-mediated endocytosis and exocytosis. virions contain two types of protein; the major 22 kda capsid protein and the minor read-through protein, p74, which is composed of the major capsid protein fuse ... | 1995 | 7882968 |
| purification and characterization of alpha-l-arabinofuranosidase from bacillus stearothermophilus t-6. | bacillus stearothermophilus t-6 produced an alpha-l-arabinofuranosidase when grown in the presence of l-arabinose, sugar beet arabinan, or oat spelt xylan. at the end of a fermentation, about 40% of the activity was extracellular, and enzyme activity in the cell-free supernatant could reach 25 u/ml. the enzymatic activity in the supernatant was concentrated against polyethylene glycol 20000, and the enzyme was purified eightfold by anion-exchange and hydrophobic interaction chromatographies. the ... | 1995 | 7887599 |
| expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid. | when subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (badh). we report here cloning and characterization of badh cdna from barley, a monocot, and the expression pattern of a badh transcript. an open reading frame of 1515 bp encoded a protein which showed high homology to badh enzymes present in other ... | 1995 | 7888620 |
| in situ localization of the non-structural protein p25 encoded by beet necrotic yellow vein virus rna 3. | the in situ localization of the non-structural protein p25 encoded by beet necrotic yellow vein virus (bnyvv) rna 3 and of the bnyvv coat protein (cp) was studied by immunoelectron microscopy in infected leaf and root cells of chenopodium murale and c. quinoa. the cp was detected in the cytoplasm of all cell types except xylem, sieve elements, and companion cells. p25 was detected in the cytoplasm and nuclei of the same cell types. the intensity of cp labelling varied depending upon the stage of ... | 1995 | 7897353 |
| restriction fragment length polymorphism analysis of reverse transcription-pcr products reveals the existence of two major strain groups of beet necrotic yellow vein virus. | beet necrotic yellow vein virus (bnyvv)-infected sugarbeets were obtained from many parts of europe and also from some sites in asia and the u.s.a. reverse transcription (rt)-pcr products of more than 1 kbp were obtained for four different regions of the viral genome which may be particularly important with respect to the pathogenic properties of the virus, i.e. for the coat protein and the 42k protein-encoding regions on rna 2 and for major parts of rnas 3 and 4. restriction fragment length pol ... | 1994 | 7913953 |
| effect of ammonia treatment of wheat straw with or without supplementation of potato protein on intake, digestion and kinetics of comminution, rumen degradation and passage in steers. | untreated wheat straw (uws) or ammoniated wheat straw without (aws) or with (awsp) a supplement of potato protein of a low rumen degradability was fed to three steers according to a 3 x 3 latin square design. all rations were supplemented with sugar-beet pulp and minerals. voluntary organic matter intake (omi, g/kg0.75 per d) was 67.8, 76.0 and 80.1 for whole rations (51.1, 59.7 and 59.2 for straw) for uws, aws and awsp respectively, which was significantly higher for aws and awsp than for uws. ... | 1994 | 7918323 |
| affinity purification of sucrose binding proteins from the plant plasma membrane. | purified plasma membranes from sugar beet leaves were solubilized by 1% 3-((3-cholamidopropyl)dimethylammonio)-1-propanesulfonate and loaded on a sepharose 6 b column substituted with sucrose. elution with sucrose at ph 5.2 yielded a peak that represented 0.2% of the loaded protein. this peak did not appear when the samples were pretreated with either 0.5 mm n-ethylmaleimide (nem) or 0.5 mm para-chloromercuribenzenesulfonic acid. it was also absent when palatinose, a sucrose analogue not recogni ... | 1994 | 7918635 |
| soil biodegradation and leaf transfer of insecticide imidacloprid applied in seed dressing in sugar beet crops. | 1994 | 7919709 | |
| actual medical and ergonomic problems in agriculture in the ukraine. | a new agrarian policy in a number of countries caused the necessity to introduce additional measures aimed at preventing of agricultural workers' health. the epidemiological studies in the ukraine revealed the prevalence of cardiovascular diseases (ischemic heart disease, arterial hypertension) particularly among operators of agricultural machines and workers being in contact with pesticides. the state of health of women working in greenhouses and those in sugar beet growing is assessed. the rat ... | 1994 | 7921900 |
| herbivore-induced volatiles: the emission of acyclic homoterpenes from leaves of phaseolus lunatus and zea mays can be triggered by a beta-glucosidase and jasmonic acid. | the treatment of healthy, undamaged plants of the lima bean phaseolus lunatus with solutions of a beta-glucosidase from bitter almonds (at 5 u.ml-1) through the petiole results in an enhanced emission of volatiles to the environment. the compounds are identical with those emitted in response to infestation with the red spotted spider mite tetranychus urticae. dominant products are the two acyclic homoterpenes 4,8-dimethyl-1,3e,7- dimethylnonatriene (homoterpene i) and 4,8,12-trimethyl-1,3e,7e,11 ... | 1994 | 7925964 |
| coat protein sequences of rmv-like strains of barley yellow dwarf virus separate them from other luteoviruses. | illinois (il) and minnesota (mn) rmv-like strains of barley yellow dwarf virus (bydv) were identified from maize displaying red leaf symptoms by enzyme-linked immunosorbent assay (elisa) using antiserum against a new york strain (bydv-rmv-ny). some il and mn strains, but not the ny strain, could be detected by elisa with a monoclonal antibody raised against bydv-rpv-ny. the region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequen ... | 1994 | 7928284 |
| the infectivity of dimeric potato yellow mosaic geminivirus clones in different hosts. | head-to-tail dimeric clones of both dna a and dna b of potato yellow mosaic geminivirus (pymv) were constructed. these constructs were infectious when inoculated onto nicotiana benthamiana plants either as dna or by agroinoculation and were also infectious for tomato plants by agroinoculation. the dimers were not infectious for potato plants following inoculation by either method. symptom induction required both dna a and dna b but agroinoculation with dna a alone resulted in virus spread in 30% ... | 1994 | 7931179 |
| on the identification of group ii introns in nucleotide sequence data. | four different consensus sequences (gti, group ii identifiers) have been derived from domains v of known group ii introns and are used as query input sequences for sensitive database screenings with the fasta and lfasta programs. the set of four gti sequences can identify all domains v of the 96 known group ii introns in the completely sequenced chloroplast genomes of marchantia polymorpha, epifagus virginiana, oryza sativa, nicotiana tabacum and the completely sequenced mitochondrial genomes of ... | 1994 | 7932698 |
| the effects of dietary sugar-beet fibre and guar gum on lipid metabolism in wistar rats. | this study investigates the mechanisms of action for the hypocholesterolaemic effects of sugar-beet fibre (sbf) and guar gum. four groups of ten male wistar rats were fed ad lib. on test diets containing either 100 g sbf or guar/kg, or control diets containing 100 g cellulose or wheat bran/kg for 28 d. food intake, weight gain and food consumption ratios were unaffected by the diets. circulating cholesterol and hepatic cholesterol concentrations were significantly lower in both sbf- and guar-fed ... | 1994 | 7947654 |
| phenotypic and genotypic diversity of fluorescent pseudomonads isolated from field-grown sugar beet. | a sample of 30 fluorescent pseudomonads isolated from the phyllosphere of sugar beet throughout a single growing season and shown to be closely related on the basis of fatty acid methyl ester (fame) analysis was subjected to detailed phenotypic and genotypic characterization. phenotypic traits were assessed on the basis of biochemical properties, assimilation of sole carbon sources, fame analysis, organic pyrolysate content (ms-pyrolysis), and total cellular protein profiles. with the exception ... | 1994 | 7952185 |