Publications
Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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intraspecific and interspecific variations in the mitochondrial genomes of nilaparvata (hemiptera: delphacidae). | planthoppers in the genus nilaparvata distant are serious pests of rice and many other crops in tropical and temperate asia, and northern australia. in this study, the mitochondrial genomes of four nilaparvata planthoppers were sequenced, three in nilaparvata lugens stål and one in nilaparvata muiri china. mitochondrial genome of nilaparvata contain the standard set of 13 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes, and a control region. the nucleotide composition of nilap ... | 2015 | 26470349 |
rnai knockdown of acetyl-coa carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, nilaparvata lugens. | a major challenge in ecology lies in understanding the coexistence of intraguild species, well documented at the organismal level, but not at the molecular level. this study focused on the effects of the antibiotic, jinggangmycin (jgm), a fungicide widely used in asian rice agroecosystems, on reproduction of insects within the planthopper guild, including the brown planthopper (bph) nilaparvata lugens and the white-backed planthopper (wbph) sogatella furcifera, both serious resurgence rice pests ... | 2015 | 26482193 |
a salivary sheath protein essential for the interaction of the brown planthopper with rice plants. | salivary secretions, including gel saliva and watery saliva, play crucial roles in the interaction between the insect and plant during feeding. in this study, we identified a salivary gland-specific gene encoding a salivary sheath protein (nlshp) in nilaparvata lugens. nlshp has two alternative splicing variants; both are expressed at high levels during the nymph and adult stages. immunohistochemical staining showed that the nlshp were synthesized in the principal gland cells of the salivary gla ... | 2015 | 26483289 |
small interfering rna pathway modulates initial viral infection in midgut epithelium of insect after ingestion of virus. | numerous viruses are transmitted in a persistent manner by insect vectors. persistent viruses establish their initial infection in the midgut epithelium, from where they disseminate to the midgut visceral muscles. although propagation of viruses in insect vectors can be controlled by the small interfering rna (sirna) antiviral pathway, whether the sirna pathway can control viral dissemination from the midgut epithelium is unknown. infection by a rice virus (southern rice black streaked dwarf vir ... | 2015 | 26537672 |
functional screen for micrornas of nilaparvata lugens reveals that targeting of glutamine synthase by mir-4868b regulates fecundity. | insect fecundity is regulated by the interaction of genotypes and the environment. micrornas (mirnas) also act in insect development and reproduction by regulating genes involved in these physiological processes. although hundreds of insect mirnas have been identified, the biological roles of most remain poorly understood. here, we used a multi-algorithm approach for mirna target prediction in 3'utrs of fecundity-related genes in the brown planthopper (bph) nilaparvata lugens and identified 38 p ... | 2015 | 26546713 |
ran involved in the development and reproduction is a potential target for rna-interference-based pest management in nilaparvata lugens. | ran (rangtpase) in insects participates in the 20-hydroxyecdysone signal transduction pathway in which downstream genes, ftz-f1, krüppel-homolog 1 (kr-h1) and vitellogenin, are involved. a putative ran gene (nlran) was cloned from nilaparvata lugens, a destructive phloem-feeding pest of rice. nlran has the typical ran primary structure features that are conserved in insects. nlran showed higher mrna abundance immediately after molting and peaked in newly emerged female adults. among the examined ... | 2015 | 26554926 |
insectbase: a resource for insect genomes and transcriptomes. | the genomes and transcriptomes of hundreds of insects have been sequenced. however, insect community lacks an integrated, up-to-date collection of insect gene data. here, we introduce the first release of insectbase, available online at http://www.insect-genome.com. the database encompasses 138 insect genomes, 116 insect transcriptomes, 61 insect gene sets, 36 gene families of 60 insects, 7544 mirnas of 69 insects, 96,925 pirnas of drosophila melanogaster and chilo suppressalis, 2439 lncrna of n ... | 2015 | 26578584 |
different pathogenicities of rice stripe virus from the insect vector and from viruliferous plants. | persistent plant viruses usually depend on insects for their transmission; they cannot be transmitted between plants or through mechanical inoculation. however, the mechanism by which persistent viruses become pathogenic in insect vectors remains unknown. in this study, we used rice stripe virus (rsv), its insect vector laodelphax striatellus and host plant (oryza sativa) to explore how persistent viruses acquire pathogenicity from insect vectors. rsv acquired phytopathogenicity in both the alim ... | 2016 | 26585422 |
omics-based comparative transcriptional profiling of two contrasting rice genotypes during early infestation by small brown planthopper. | the small brown planthopper (sbph) is one of the destructive pests of rice. although different biochemical pathways that are involved in rice responding to planthopper infestation have been documented, it is unclear which individual metabolic pathways are responsive to planthopper infestation. in this study, an omics-based comparative transcriptional profiling of two contrasting rice genotypes, an sbph-resistant and an sbph-susceptible rice line, was assessed for rice individual metabolic pathwa ... | 2015 | 26633389 |
application of brown planthopper salivary gland extract to rice plants induces systemic host mrna patterns associated with nutrient remobilization. | insect saliva plays an important role in modulation of plant-insect interactions. although this area of research has generated much attention in recent years, mechanisms of how saliva affects plant responses remain poorly understood. to address this void, the present study investigated the impact of the brown planthopper (nilaparvata lugens, stål; hereafter bph) salivary gland extract (sge) on rice (oryza sativa) systemic responses at the mrna level. differentially expressed rice mrnas were gene ... | 2015 | 26641488 |
evolving ideas about genetics underlying insect virulence to plant resistance in rice-brown planthopper interactions. | many plant-parasite interactions that include major plant resistance genes have subsequently been shown to exhibit features of gene-for-gene interactions between plant resistance genes and parasite avirulence genes. the brown planthopper (bph) nilaparvata lugens is an important pest of rice (oryza sativa). historically, major resistance genes have played an important role in agriculture. as is common in gene-for-gene interactions, evolution of bph virulence compromises the effectiveness of singl ... | 2016 | 26668110 |
organ-specific transcriptome response of the small brown planthopper toward rice stripe virus. | rice stripe virus (rsv) causes rice stripe disease and is transmitted by the small brown planthopper (laodelphax striatellus, sbph) in a persistent, circulative, and propagative manner. the alimentary canal and salivary gland of sbph play important roles in viral replication and transmission. however, little is known about the underlying molecular functions of these two organs in the interaction between rsv and sbph. in this study, organ-specific transcriptomes of the alimentary canal and saliva ... | 2016 | 26678499 |
foxo links wing form polyphenism and wound healing in the brown planthopper, nilaparvata lugens. | polyphenisms such as wing dimorphisms and caste determination are important in allowing animals to adapt to changing environments. the brown planthopper nilaparvata lugens, one of the most serious insect agricultural pests, includes two wing forms, the long wing form (macropterous) and the short wing form (brachypterous). long wings are specialized for migration, while short wings are found in individuals specialized for reproduction. while studying wing form polyphenism in the brown planthopper ... | 2016 | 26696545 |
genetic basis of multiple resistance to the brown planthopper (nilaparvata lugens stål) and the green rice leafhopper (nephotettix cincticeps uhler) in the rice cultivar 'asd7' (oryza sativa l. ssp. indica). | the rice cultivar asd7 (oryza sativa l. ssp. indica) is resistant to the brown planthopper (bph; nilaparvata lugens stål) and the green leafhopper (nephotettix virescens distant). here, we analyzed multiple genetic resistance to bph and the green rice leafhopper (grh; nephotettix cincticeps uhler). using two independent f2 populations derived from a cross between asd7 and taichung 65 (oryza sativa ssp. japonica), we detected two qtls (qbph6 and qbph12) for resistance to bph and one qtl (qgrh5) f ... | 2015 | 26719745 |
evidence for the presence of biogenic magnetic particles in the nocturnal migratory brown planthopper, nilaparvata lugens. | biogenic magnetic particles have been detected in some migratory insects, which implies the basis of magnetoreception mechanism for orientation and navigation. here, the biogenic magnetic particles in the migratory brown planthopper (bph), nilaparvata lugens were qualitatively measured by squid magnetometry, and their characteristics were further determined by prussian blue staining, electron microscopy and energy dispersive x-ray spectroscopy. the results indicate that there were remarkable mag ... | 2016 | 26727944 |
comparing gene expression profiles between bt and non-bt rice in response to brown planthopper infestation. | bt proteins are the most widely used insecticidal proteins in transgenic crops for improving insect resistance. we previously observed longer nymphal developmental duration and lower fecundity in brown planthopper (bph) fed on bt rice line kmd2, although bt insecticidal protein cry1ab could rarely concentrate in this non-target rice pest. in the present study, we performed microarray analysis in an effort to detect bt-independent variation, which might render bt rice more defensive and/or less n ... | 2015 | 26734057 |
adipose triglyceride lipase (atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, nilaparvata lugens stål. | the antibiotic jinggangmycin (jgm) is an agrochemical product widely used in china for controlling rice sheath blight, rhizoctonia solani. unexpectedly, it stimulates reproduction in the brown planthopper (bph), nilaparvata lugens (stål). however, the underlying molecular mechanisms of the stimulation are unclear. the present investigation demonstrates that adipose triglyceride lipase (atgl) is one of the enzymes involved in the jgm-stimulated reproduction in bph. silence of atgl in jgm-treated ... | 2016 | 26739506 |
a cripavirus in the brown planthopper, nilaparvata lugens. | a cripavirus-like long unique sequence was identified during transcriptome sequencing of the brown planthopper (bph), nilaparvata lugens. this unique sequence demonstrated high similarity with the whole-genome sequence of cricket paralysis virus, including 5' and 3' untranslated regions; thus we considered it the whole genome of a new virus. we propose that the virus be named nilaparvata lugens c virus (nlcv). the plus-strand rna genome spanned 9144 nt, excluding a 3' poly(a) tail with two large ... | 2016 | 26746854 |
characteristics of the draft genome of "candidatus arsenophonus nilaparvatae", a facultative endosymbiont of nilaparvata lugens. | there exists a kind of symbiotic bacterium named "candidatus arsenophonus nilaparvatae" in the brown planthopper (bph), nilaparvata lugens. after being filtered and assembled from the bph genome sequencing project, the genome sequence of this bacterial symbiont was obtained. after initial analysis based on the genome, we have found its potential role to synthesize b vitamins for the host. in order to better understand the lifestyle and the genomic changes of this symbiotic bacterium after the sy ... | 2016 | 26792263 |
knockdown of nadph-cytochrome p450 reductase results in reduced resistance to buprofezin in the small brown planthopper, laodelphax striatellus (fallén). | nadph-cytochrome p450 reductase (cpr) plays an important role in cytochrome p450 function, and cpr knockdown in several insects leads to increased susceptibility to insecticides. however, a putative cpr gene has not yet been fully characterized in the small brown planthopper laodelphax striatellus, a notorious agricultural pest in rice that causes serious damage by transmitting rice stripe and rice black-streaked dwarf viruses. the objective of this study was to clone the cdna and to knock down ... | 2016 | 26821654 |
characterization of actin and tubulin promoters from two sap-sucking pests, nilaparvata lugens (stål) and nephotettix cincticeps (uhler). | the brown planthopper, nilaparvata lugens (n. lugens, hemiptera: delphacidae), and the green rice leafhopper, nephotettix cincticeps (n. cincticeps, hemiptera: cicadellidae), two sap-sucking feeders, have caused many destructive agricultural disasters in east asia, as they can bring diseases like 'hopper burn' and transmit plant viruses. recently, continuously-cultured cell lines from both insects have been reported. however, exogenous protein expression systems have not yet been established. he ... | 2016 | 26826390 |
loop replacements with gut-binding peptides in cry1ab domain ii enhanced toxicity against the brown planthopper, nilaparvata lugens (stål). | bacillus thuringiensis (bt) cry toxins have been used widely in pest managements. however, cry toxins are not effective against sap-sucking insects (hemiptera), which limits the application of bt for pest management. in order to extend the insecticidal spectrum of bt toxins to the rice brown planthopper (bph), nilaparvata lugens, we modified cry1ab putative receptor binding domains with selected bph gut-binding peptides (gbps). three surface exposed loops in the domain ii of cry1ab were replaced ... | 2016 | 26830331 |
novel hydrazone derivatives containing pyridine amide moiety: design, synthesis, and insecticidal activity. | a series of novel hydrazone derivatives containing pyridine amide moiety were designed, synthesized, and evaluated for their insecticidal activity. bioassays indicated that some of the target compounds exhibited good insecticidal activities against nilaparvata lugens (n. lugens), plutella xylostella (p. xylostella), mythimna separata (m. separata), helicoverpa armigera (h. armigera), pyrausta nubilalis (p. nubilalis), and culex pipiens pallens (c. pipiens pallens). in particular, compound 5j rev ... | 2016 | 26832221 |
geographic variation of diapause and sensitive stages of photoperiodic response in laodelphax striatellus fallén (hemiptera: delphacidae). | large numbers of the small brown planthopper laodelphax striatellus (fallén) (hemiptera: delphacidae) occur in temperate regions, causing severe losses in rice, wheat, and other economically important crops. the planthoppers enter diapause in the third- or fourth-instar nymph stage, induced by short photoperiods and low temperatures. to investigate the geographic variation in l. striatellus diapause, we compared the incidence of nymphal diapause under various constant temperature (20 and 27 °c) ... | 2016 | 26839318 |
complete genomic sequence of maize rough dwarf virus, a fijivirus transmitted by the small brown planthopper. | the nucleotide sequences of the 10 genomic segments of an italian isolate of maize rough dwarf virus (mrdv) were determined. this first complete genomic sequence of mrdv will help understand the phylogenetic relationships among group 2 fijiviruses and especially the closely related rice black-streaked dwarf virus, which is also found to naturally infect maize. | 2016 | 26847903 |
viruliferous rate of small brown planthopper is a good indicator of rice stripe disease epidemics. | rice stripe virus (rsv), its vector insect (small brown planthopper, sbph) and climatic conditions in jiangsu, china were monitored between 2002 and 2012 to determine key biotic and abiotic factors driving epidemics of the disease. average disease severity, disease incidence and viruliferous rate of sbph peaked in 2004 and then gradually decreased. disease severity of rsv was positively correlated with viruliferous rate of the vector but not with the population density of the insect, suggesting ... | 2016 | 26898155 |
a comprehensive assessment of the effects of transgenic cry1ac/cry1ab rice huahui 1 on adult micraspis discolor (fabricius) (coleoptera: coccinellidae). | micraspis discolor (fabricius) (coleoptera: coccinellidae) is a widely distributed coleoptera predator in southern asia in rice ecosystem, and adult m. discolor feed on both rice pollen and soft-bodied arthropods. bitrophic bioassay and tritrophic bioassay were conducted to evaluate the potential impact of cry1ac/cry1ab-expressing rice huahui 1 and its non-transgenic counterpart minghui 63 on fitness parameters of adult m. discolor. the results showed that the survival, and fecundity of this bee ... | 2016 | 26914608 |
nutritional signaling regulates vitellogenin synthesis and egg development through juvenile hormone in nilaparvata lugens (stål). | insect female reproduction which comprises the synthesis of vitellogenein (vg) in the fat body and its incorporation into developing oocytes, needs a large amount of energy and food resources. our previous studies found that juvenile hormone (jh) regulates vitellogenesis in the brown planthopper, nilaparvata lugens. here, we report on the role of jh in nutrient-regulated vg synthesis and egg development. we first cloned the genes coding for juvenile hormone acid methyltransferase (jhamt) which i ... | 2016 | 26927076 |
identification of a heat shock protein 90 gene involved in resistance to temperature stress in two wing-morphs of nilaparvata lugens (stål). | the brown planthopper, nilaparvata lugens, is one of the most destructive pests damaging rice in asia and exhibits wing dimorphism, with brachypters possessing severely reduced wings and macropters bearing fully developed wings. previous studies have shown that macropters are more heat resistant than brachypters. to understand the molecular mechanism underlying the differential thermotolerance abilities of these two morphs, a full-length hsp gene, nlhsp90 was cloned from n. lugen. our results sh ... | 2016 | 26945596 |
effects of fungicides on the yeast-like symbiotes and their host, nilaparvata lugens stål (hemiptera: delphacidae). | yeast-like symbiotes (yls) are endosymbionts that are closely related to the growth, development and reproduction of their host, the brown planthopper (bph), nilaparvata lugens stål (hemiptera: delphacidae). in order to understand the relationship between the population of yls in bph cells and the survival rate of bph, eight different fungicides were applied to rice plants infested by bph, and the number of yls and mortality of bph were determined. three of the fungicides, 27% toyocamycin & tetr ... | 2016 | 26969435 |
silencing of aco decreases reproduction and energy metabolism in triazophos-treated female brown plant hoppers, nilaparvata lugens stål (hemiptera: delphacidae). | the brown plant hopper (bph), nilaparvata lugens stål (hemiptera: delphacidae), is a major pest affecting rice in asia, and outbreaks of this pest are closely linked to pesticide-induced stimulation of reproduction. therefore, the bph is a classic example of a resurgent pest. however, the effects of different genes on the regulation of pesticide-induced reproductive stimulation in the bph are unclear. in this study, the regulatory effects of acyl-coenzyme a oxidase (aco) on the reproduction and ... | 2016 | 26969443 |
genetic and biochemical mechanisms of rice resistance to planthopper. | this article presents a comprehensive review on the genetic and biochemical mechanisms governing rice-planthopper interactions, aiming to contribute substantial planthopper control and facilitate breeding for resistance to planthoppers in rice. the rice planthopper is the most destructive pest of rice and a substantial threat to rice production. the brown planthopper (bph), white-backed planthopper (wbph) and small brown planthopper (sbph) are three species of delphacid planthoppers and importan ... | 2016 | 26979747 |
the roles of cyp6ay1 and cyp6er1 in imidacloprid resistance in the brown planthopper: expression levels and detoxification efficiency. | two p450 monooxygenase genes, cyp6ay1 and cyp6er1, were reported to contribute importantly to imidacloprid resistance in the brown planthopper, nilaparvata lugens. although recombinant cyp6ay1 could metabolize imidacloprid efficiently, the expression levels of cyp6er1 gene were higher in most resistant populations. in the present study, three field populations were collected from different countries, and the bioassay, rnai and imidacloprid metabolism were performed to evaluate the importance of ... | 2016 | 27017884 |
oswrky53, a versatile switch in regulating herbivore-induced defense responses in rice. | wrky proteins, which belong to a large family of plant-specific transcription factors, play important roles in plant defenses against pathogens and herbivores by regulating defense-related signaling pathways. recently, a rice wrky transcription factor oswrky53 has been reported to function as a negative feedback modulator of osmpk3/osmpk6 and thereby to control the size of the investment a rice plant makes to defend against a chewing herbivore, the striped stem borer chilo suppressalis. we inves ... | 2016 | 27031005 |
tor pathway-mediated juvenile hormone synthesis regulates nutrient-dependent female reproduction in nilaparvata lugens (stål). | the "target of rapamycin" (tor) nutritional signaling pathway and juvenile hormone (jh) regulation of vitellogenesis has been known for a long time. however, the interplay between these two pathways regulating vitellogenin (vg) expression remains obscure. here, we first demonstrated the key role of amino acids (aas) in activation of vg synthesis and egg development in nilaparvata lugens using chemically defined artificial diets. aas induced the expression of tor and s6k (s6 kinase), whereas rnai ... | 2016 | 27043527 |
knockdown of two trehalose-6-phosphate synthases severely affects chitin metabolism gene expression in the brown planthopper nilaparvata lugens. | rna interference combined with digital gene expression (dge) analysis can be used to study gene function. trehalose-6-phosphate synthase (tps) plays a key role in the synthesis of trehalose and insect development. | 2017 | 27060284 |
identification of the secreted watery saliva proteins of the rice brown planthopper, nilaparvata lugens (stål) by transcriptome and shotgun lc-ms/ms approach. | the rice brown planthopper, nilaparvata lugens (stål), a major rice insect pest in asia, is a vascular bundle-feeder that ejects gelling and watery saliva during the feeding process. although major proteins in the salivary glands of n. lugens have been identified using 2d page, very little is known about the secreted saliva of this insect. in this study, we identified the major proteins in the secreted watery saliva of n. lugens, via collecting from a sucrose diet that adult planthoppers had fed ... | 2016 | 27080912 |
seasonal changes in the percentage of rice stripe virus viruliferous laodelphax striatellus (hemiptera: delphacidae) in paddy fields in japan. | rice stripe disease, which is caused by rice stripe virus (rsv), is one of the most serious viral diseases of rice. rsv is transmitted in a persistent manner bylaodelphax striatellus(fallén). the incidence of the disease can be estimated from the density of viruliferous vectors. understanding seasonal changes of the percentage of viruliferousl. striatelluscan facilitate forecasting and controlling the disease. in paddies, the percentage of viruliferous insects fluctuated in phase with the rate o ... | 2016 | 27099363 |
jnk signaling mediates wing form polymorphism in brown planthoppers (nilaparvata lugens). | wing polyphenism is considered to be an adaptive trade-off between migration (long winged forms) and reproduction (short winged forms), determined by various environmental conditions. the c-jun nh2-terminal kinase (jnk) is crucial for the regulation of the activity of a number of transcription factors, and is activated under stress and environmental fluctuations where it functions in maintaining cell viability and proliferation. we used rna interference and a pharmacological inhibitor of jnk to ... | 2016 | 27120575 |
mode of action of triflumezopyrim: a novel mesoionic insecticide which inhibits the nicotinic acetylcholine receptor. | triflumezopyrim, a newly commercialized molecule from dupont crop protection, belongs to the novel class of mesoionic insecticides. this study characterizes the biochemical and physiological action of this novel insecticide. using membranes from the aphid, myzus persicae, triflumezopyrim was found to displace (3)h-imidacloprid with a ki value of 43 nm with competitive binding results indicating that triflumezopyrim binds to the orthosteric site of the nicotinic acetylcholine receptor (nachr). in ... | 2016 | 27130855 |
screening and functional analyses of nilaparvata lugens salivary proteome. | most phloem-feeding insects secrete gelling and watery saliva during the feeding process. however, the functions of salivary proteins are poorly understood. in this study, our purpose was to reveal the components and functions of saliva in a rice sap-sucking insect pest, nilaparvata lugens. the accomplishment of the whole genome and transcriptome sequencing in n. lugens would be helpful for elucidating the gene information and expression specificity of the salivary proteins. in this study, we ha ... | 2016 | 27142481 |
ecological trade-offs between migration and reproduction are mediated by the nutrition-sensitive insulin-signaling pathway. | crowding and changes in food availability are two critical environmental conditions that impact an animal's trajectory toward either migration or reproduction. many insects facing this challenge have evolved wing polyphenisms. when conditions favor reproduction, wing polyphenic species produce adults that either have no wings or short, non-functional wings. facultative wing growth reflects a physiological and evolutionary trade-off between migration and reproduction, triggered by environmental c ... | 2016 | 27143957 |
no cross-resistance between imidacloprid and pymetrozine in the brown planthopper: status and mechanisms. | cross-resistance between insecticides, especially from different groups, can be extremely unpredictable, and it has been a serious concern in pest control. pymetrozine has been widely used to control nilaparvata lugens with the suspension of imidacloprid for the resistance, and n. lugens has showed obvious pymetrozine resistance in recent years. to investigate the possible cross-resistance between imidacloprid and pymetrozine is very important to avoid the adverse effects on resistance developme ... | 2016 | 27155488 |
response of adult brown planthopper nilaparvata lugens (stål) to rice nutrient management. | nitrogen (n) limitation is well documented for the brown planthopper (bph) nilaparvata lugens (stål), but phosphorus (p) and potassium (k) limitation is poorly studied. we studied the effects of n, p, and k application on chemical composition of rice plants and its consequences on life parameters-adult longevity, fecundity, and egg hatchability of bph. life parameters of bph were regressed as function of plant chemical composition. a completely randomized design with four replicates in a factori ... | 2016 | 27155974 |
characterization of heat shock cognate protein 70 gene and its differential expression in response to thermal stress between two wing morphs of nilaparvata lugens (stål). | previous studies have demonstrated differences in thermotolerance between two wing morphs of nilaparvata lugens, the most serious pest of rice across the asia. to reveal the molecular regulatory mechanisms underlying the differential thermal resistance abilities between two wing morphs, a full-length of transcript encoding heat shock cognate protein 70 (hsc70) was cloned, and its expression patterns across temperature gradients were analyzed. the results showed that the expression levels of nlhs ... | 2016 | 27181274 |
temporal interactions of plant - insect - predator after infection of bacterial pathogen on rice plants. | pathogenic infection on plants may affect interactions of host-plants with their herbivores, as well as the herbivores with their predators. in this study, the effects of infection by pathogenic bacterium xanthomonas oryzae pv. oryzae (xoo), which causes a vascular disease in rice, on rice plants and consequent interactions with a rice herbivore, brown rice planthopper (bph) nilaparvata lugens, and its major predator, cyrtorhinus lividipennis, were investigated. the results showed that the rice ... | 2016 | 27185548 |
marker assisted pyramiding of two brown planthopper resistance genes, bph3 and bph27 (t), into elite rice cultivars. | brown planthopper (bph) is the most destructive insect in rice production. breeding of resistant cultivars is the most cost-effective and environment-friendly strategy for bph management; however, resistant cultivars are currently hampered by the rapid breakdown of bph resistance. thus, there is an urgent need to use more effective bph resistance genes or pyramiding different resistance genes to develop more durable resistant rice cultivars. | 2016 | 27246014 |
transmission electron microscopy (tem) observations of female oocytes from nilaparvata lugens (hemiptera: delphacidae): antibiotic jinggangmycin (jgm)-induced stimulation of reproduction and associated changes in hormone levels. | previous studies have demonstrated that the agricultural antibiotic jinggangmycin (jgm) stimulates reproduction in the brown planthopper nilaparvata lugens stål and that the stimulation of brown planthopper reproduction induced by jgm is regulated by the fatty acid synthase (fas) and acetyl-coa carboxylase (acc) genes. however, a key issue in the stimulation of reproduction induced by pesticides involves the growth and development of oocytes. therefore, the present study investigated oocyte chan ... | 2016 | 27247297 |
the transcription factor oswrky45 negatively modulates the resistance of rice to the brown planthopper nilaparvata lugens. | wrky transcription factors play a central role not only in plant growth and development but also in plant stress responses. however, the role of wrky transcription factors in herbivore-induced plant defenses and their underlying mechanisms, especially in rice, remains largely unclear. here, we cloned a rice wrky gene oswrky45, whose expression was induced by mechanical wounding, by infestation of the brown planthopper (bph, nilaparvata lugens) and by treatment with jasmonic acid (ja) or salicyli ... | 2016 | 27258255 |
differential proteomics profiling of the ova between healthy and rice stripe virus-infected female insects of laodelphax striatellus. | rice stripe virus-infected females of the small brown planthopper (sbph, laodelphax striatellus) usually lay fewer eggs with a longer hatch period, low hatchability, malformation and retarded or defective development compared with healthy females. to explore the molecular mechanism of those phenomena, we analyzed the differential proteomics profiling of the ova between viruliferous and healthy female insects using an isobaric tag for relative and absolute quantitation (itraq) approach. we obtain ... | 2016 | 27277140 |
specific synergist for neonicotinoid insecticides: ippa08, a cis-neonicotinoid compound with a unique oxabridged substructure. | insecticide synergists are key components to increase the control efficacy and reduce active ingredient use. here, we describe a novel insecticide synergist with activity specific for insecticidal neonicotinoids. the synergist ippa08, a cis configuration neonicotinoid compound with a unique oxabridged substructure, could increase the toxicity of most neonicotinoid insecticides belonging to the insecticide resistance action committee (irac) 4a subgroup against a range of insect species, although ... | 2016 | 27281691 |
recent progress on the genetics and molecular breeding of brown planthopper resistance in rice. | brown planthopper (bph) is the most devastating pest of rice. host-plant resistance is the most desirable and economic strategy in the management of bph. to date, 29 major bph resistance genes have been identified from indica cultivars and wild rice species, and more than ten genes have been fine mapped to chromosome regions of less than 200 kb. four genes (bph14, bph26, bph17 and bph29) have been cloned. the increasing number of fine-mapped and cloned genes provide a solid foundation for develo ... | 2016 | 27300326 |
expression pattern and pharmacological characterisation of two novel alternative splice variants of the glutamate-gated chloride channel in the small brown planthopper laodelphax striatellus. | glutamate-gated chloride channels (glucl) mediate fast inhibitory neurotransmission in invertebrate nervous systems. although only one glucl gene was presented in insects, it showed diverse alternative splicing that was speculated could affect channel function and pharmacology. | 2017 | 27302648 |
suppressing male spermatogenesis-associated protein 5-like gene expression reduces vitellogenin gene expression and fecundity in nilaparvata lugens stål. | in our previous study with the brown planthopper (bph), nilaparvata lugens, triazophos (tzp) treatments led to substantial up-regulation of a male spermatogenesis-associated protein 5-like gene (nlspata5) compared to untreated controls. mating with tzp-treated males significantly increased fecundity (as numbers of eggs laid), relative to females mated with untreated males. because spata5 acts in mammalian sperm development and is expressed in testes, we posed the hypothesis that nlspata5 occurs ... | 2016 | 27305948 |
development of oryza sativa l. by oryza punctata kotschy ex steud. monosomic addition lines with high value traits by interspecific hybridization. | this paper describes the development of monosomic alien addition and disomic introgression lines through a cross between autotetraploid indica rice and oryza punctata toward tapping valuable traits for rice improvement. oryza punctata is a distantly related wild oryza species having bb genome with untapped genetic resources for rice improvement. low crossability between the cultivated o. sativa and o. punctata restricts the success of transferring many desirable traits into cultivated rice. arti ... | 2016 | 27318700 |
functional characterization of three trehalase genes regulating the chitin metabolism pathway in rice brown planthopper using rna interference. | rna interference (rnai) is an effective gene-silencing tool, and double stranded rna (dsrna) is considered a powerful strategy for gene function studies in insects. in the present study, we aimed to investigate the function of trehalase (tre) genes (tre 1-1, tre 1-2, and tre-2) isolated from the brown planthopper nilaparvata lugens, a typical piercing-sucking insect in rice, and investigate their regulating roles in chitin synthesis by injecting larvae with dsrna. the results showed that tre1 an ... | 2016 | 27328657 |
atp phosphoribosyltransferase from symbiont entomomyces delphacidicola invovled in histidine biosynthesis of nilaparvata lugens (stål). | histidine is an essential amino acid assumed to be synthesized by an obligatory yeast-like symbiont (entomomyces delphacidicola str. nlu) in nilaparvata lugens, an important rice pest. the adenosine-triphosphate phosphoribosyltransferase (atp-prtase) facilities the committed first step of the histidine biosynthesis pathway. in the current study, a putative atp-prtase was cloned and verified to be of e. delphacidicola origin (edeprtase). the expression of the gene was spatial and temporal univers ... | 2016 | 27373692 |
no impact of transgenic cry1c rice on the rove beetle paederus fuscipes, a generalist predator of brown planthopper nilaparvata lugens. | t1c-19 is newly developed transgenic rice active against lepidopteran pests, and expresses a synthesized cry1c gene driven by the maize ubiquitin promoter. the brown planthopper, nilaparvata lugens, is a major non-target pest of rice, and the rove beetle (paederus fuscipes) is a generalist predator of n. lugens nymphs. as p. fuscipes may be exposed to the cry1c protein through preying on n. lugens, it is essential to assess the potential effects of transgenic cry1c rice on this predator. in this ... | 2016 | 27444416 |
cry1ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider pardosa pseudoannulata. | the impact of bacillus thuringiensis (bt) toxin proteins on non-target predatory arthropods is not well understood at the cellular and molecular levels. here, we investigated the potential effects of cry1ab expressing rice on fecundity of the wolf spider, pardosa pseudoannulata, and some of the underlying molecular mechanisms. the results indicated that brown planthoppers (bphs) reared on cry1ab-expressing rice accumulated the cry toxin and that reproductive parameters (pre-oviposition period, p ... | 2016 | 27452121 |
discovery and functional identification of fecundity-related genes in the brown planthopper by large-scale rna interference. | recently, transcriptome and proteome data have increasingly been used to identify potential novel genes related to insect phenotypes. however, there are few studies reporting the large-scale functional identification of such genes in insects. to identify novel genes related to fecundity in the brown planthopper (bph), nilaparvata lugens, 115 genes were selected from the transcriptomic and proteomic data previously obtained from high- and low-fecundity populations in our laboratory. the results o ... | 2016 | 27472833 |
ion transport peptide (itp) regulates wing expansion and cuticle melanism in the brown planthopper, nilaparvata lugens. | ion transport peptide (itp) and its alternatively spliced homologous itp-like (itpl) products play important roles in various insect developmental processes. we found for the first time that alternative 5' untranslated regions (5' utrs) of itpl (nilluitpls-1, -2, -3 and -4) control spatiotemporal expression in the brown planthopper, nilaparvata lugens, as demonstrated by reverse-transcription quantitative pcr. by using an alternative 5' utr, nilluitpl-1 was expressed exclusively in the male repr ... | 2016 | 27515909 |
insecticide resistance monitoring and correlation analysis of insecticides in field populations of the brown planthopper nilaparvata lugens (stål) in china 2012-2014. | the brown planthopper is a serious rice pest in china. chemical insecticides have been considered a satisfactory means of controlling the brown planthopper. in the present study, we determined the susceptibility of twenty-one populations of nilaparvata lugens to eleven insecticides by a rice-stem dipping method from 2012 to 2014 in eight provinces of china. these field-collected populations of n. lugens had developed high levels of resistance to imidacloprid (resistant ratio, rr=233.3-2029-fold) ... | 2016 | 27521908 |
expression induction of p450 genes by imidacloprid in nilaparvata lugens: a genome-scale analysis. | the overexpression of p450 monooxygenase genes is a main mechanism for the resistance to imidacloprid, a representative neonicotinoid insecticide, in nilaparvata lugens (brown planthopper, bph). however, only two p450 genes (cyp6ay1 and cyp6er1), among fifty-four p450 genes identified from bph genome database, have been reported to play important roles in imidacloprid resistance until now. in this study, after the confirmation of important roles of p450s in imidacloprid resistance by the synergi ... | 2016 | 27521914 |
seminal fluid protein genes of the brown planthopper, nilaparvata lugens. | seminal fluid proteins (sfps) are produced mainly in the accessory gland of male insects and transferred to females during mating, in which they induce numerous physiological and post-mating behavioral changes. the brown plant hopper (bph), nilaparvata lugens, is an economically important hemipterous pest of rice. the behavior and physiology of the female of this species is significantly altered by mating. sfps in hemipteran species are still unclear. | 2016 | 27538518 |
synergistic and compensatory effects of two point mutations conferring target-site resistance to fipronil in the insect gaba receptor rdl. | insecticide resistance can arise from a variety of mechanisms, including changes to the target site, but is often associated with substantial fitness costs to insects. here we describe two resistance-associated target-site mutations that have synergistic and compensatory effects that combine to produce high and persistent levels of resistance to fipronil, an insecticide targeting on γ-aminobytyric acid (gaba) receptors. in nilaparvata lugens, a major pest of rice crops in many parts of asia, we ... | 2016 | 27557781 |
systemic stereoselectivity study of flufiprole: stereoselective bioactivity, acute toxicity and environmental fate. | in this study, the stereoselectivity of flufiprole enantiomers in regards to their bioactivity, acute toxicity and environmental fate is reported for the first time. four types of representative insects (plutella xylostella, nilaparvata lugens, mythimna separata and acyrthosiphon pisum) were used to investigate enantioselective bioactivity. acute toxicities of flufiprole enantiomers toward two non-target organisms were also evaluated. moreover, stereoselective degradation in four vegetables unde ... | 2016 | 27585281 |
multiple cis-acting elements involved in up-regulation of a cytochrome p450 gene conferring resistance to deltamethrin in smal brown planthopper, laodelphax striatellus (fallén). | as well as arising from single point mutations in binding sites or detoxifying enzymes, it is likely that insecticide resistance mechanisms are frequently controlled by multiple genetic factors, resulting in resistance being inherited as a quantitative trait. however, empirical evidence for this is still rare. here we analyse the causes of up-regulation of cyp6fu1, a monoxygenase implicated in resistance to deltamethrin in the rice pest laodelphax striatellus. the 5'-flanking region of this gene ... | 2016 | 27590347 |
research advances on animal genetics in china in 2015. | chinese scientists have made significant achievements in the field of animal genetics in 2015. incomplete statistics show that among all the publications of 2015 involving nematode (caenorhabditis elegans), fly (drosophila melanogaster), zebrafish (danio rerio), african clawed frog (xenopus) or mice (mus musculus), about 1/5 publications are from china. many innovative studies were published in high-impact international academic journals by chinese scientists, including the identification of a p ... | 2016 | 27655312 |
map-based cloning and characterization of the bph18 gene from wild rice conferring resistance to brown planthopper (bph) insect pest. | brown planthopper (bph) is a phloem sap-sucking insect pest of rice which causes severe yield loss. we cloned the bph18 gene from the bph-resistant introgression line derived from the wild rice species oryza australiensis. map-based cloning and complementation test revealed that the bph18 encodes cc-nbs-nbs-lrr protein. bph18 has two nbs domains, unlike the typical nbs-lrr proteins. the bph18 promoter::gus transgenic plants exhibited strong gus expression in the vascular bundles of the leaf shea ... | 2016 | 27682162 |
characterization and comparative profiling of the small rna transcriptomes in the hemipteran insect nilaparvata lugens. | micrornas (mirnas) are a group of small rnas involved in various biological processes through negative regulation of mrnas at the post-transcriptional level. the brown planthopper (bph), nilaparvata lugens (stål), is one of the most serious and destructive insect pests of rice. in the present study, two small rna libraries of virulent n. lugens populations (biotype i survives on susceptive rice variety tn1 and biotype y survives on moderately resistant rice variety yhy15) were constructed and se ... | 2016 | 27693372 |
molecular breeding of rice restorer lines and hybrids for brown planthopper (bph) resistance using the bph14 and bph15 genes. | the development of hybrid rice is a practical approach for increasing rice production. however, the brown planthopper (bph), nilaparvata lugens stål, causes severe yield loss of rice (oryza sativa l.) and can threaten food security. therefore, breeding hybrid rice resistant to bph is the most effective and economical strategy to maintain high and stable production. fortunately, numerous bph resistance genes have been identified, and abundant linkage markers are available for molecular marker-ass ... | 2016 | 27704482 |
functional analysis of cyp6er1, a p450 gene associated with imidacloprid resistance in nilaparvata lugens. | the cytochrome p450 cyp6er1 has been reported to play an important role in imidacloprid resistance of the brown planthopper (bph), nilaparvata lugens, and is overexpressed in most resistant populations. in the present study, we confirmed that cyp6er1 expression can be induced by certain levels of imidacloprid. developmental expression analysis revealed that cyp6er1 was expressed highly in the adult stage, and tissue distribution analysis showed that cyp6er1 was expressed mainly in the fat body a ... | 2016 | 27721443 |
the speed of tubule formation of two fijiviruses corresponds with their dissemination efficiency in their insect vectors. | rice black-streaked dwarf virus (rbsdv) and southern rice black-streaked dwarf virus (srbsdv) are two closely related fijiviruses transmitted by the small brown planthopper (sbph) and white-backed planthopper (wbph), respectively. srbsdv has a latent period 4 days shorter than that of rbsdv, implying a more efficient spread in insect vector. currently, the mechanisms underlying this higher efficiency are poorly understood. however, our recent studies have implicated a role of virus induced tubul ... | 2016 | 27760544 |
allelic diversity in an nlr gene bph9 enables rice to combat planthopper variation. | brown planthopper (bph), nilaparvata lugens stål, is one of the most devastating insect pests of rice (oryza sativa l.). currently, 30 bph-resistance genes have been genetically defined, most of which are clustered on specific chromosome regions. here, we describe molecular cloning and characterization of a bph-resistance gene, bph9, mapped on the long arm of rice chromosome 12 (12l). bph9 encodes a rare type of nucleotide-binding and leucine-rich repeat (nlr)-containing protein that localizes t ... | 2016 | 27791169 |
ippa08 allosterically enhances the action of imidacloprid on nicotinic acetylcholine receptors. | our previous study showed that ippa08, a cis-configuration neonicotinoid compound with unique oxabridged substructure, acted as a specific synergist to neonicotinoid insecticides targeting nicotinic acetylcholine receptors (nachrs). heteropentamer nachrs have diverse characteristics and can form canonical and noncanonical subunit interfaces. while canonical interfaces have been exploited as targets of many drugs, noncanonical interfaces have received less attention. in this study, the mechanism ... | 2016 | 27793626 |
metabolic imidacloprid resistance in the brown planthopper, nilaparvata lugens, relies on multiple p450 enzymes. | target insensitivity contributing to imidacloprid resistance in nilaparvata lugens has been reported to occur either through point mutations or quantitative change in nicotinic acetylcholine receptors (nachrs). however, the metabolic resistance, especially the enhanced detoxification by p450 enzymes, is the major mechanism in fields. from one field-originated n. lugens population, an imidacloprid resistant strain g25 and a susceptible counterpart s25 were obtained to analyze putative roles of p4 ... | 2016 | 27793627 |
molecular characterization, expression analysis and rnai knock-down of elongation factor 1α and 1γ from nilaparvata lugens and its yeast-like symbiont. | in the present paper, four cdnas encoding the alpha and gamma subunits of elongation factor 1 (ef-1) were cloned and sequenced from nilaparvata lugens, named nlef-1α, nlef-1γ, and its yeast-like symbiont (yls), named ysef-1α and ysef-1γ, respectively. comparisons with sequences from other species indicated a greater conservation for ef-1α than for ef-1γ. nlef-1α has two identical copies. the deduced amino acid sequence homology of nlef-1α and nlef-1γ is 96 and 64%, respectively, compared with ho ... | 2016 | 27809951 |
effects of nitrogen on egg-laying inhibition and ovicidal response in planthopper-resistant rice varieties. | a series of experiments was set up to examine the effects of nitrogen on rice (oryza sativa l.) resistance against nilaparvata lugens (stål) and sogatella furcifera (horváth). egg laying by n. lugens was reduced on the indica variety ir60. nymph biomass (n. lugens and s. furcifera) was also lower on ir60: this was associated with low honeydew production and a high proportion of xylem-derived honeydew in n. lugens but not in s. furcifera. nitrogen increased egg-laying by s. furcifera and increase ... | 2016 | 27812236 |
erratum: map-based cloning and characterization of the bph18 gene from wild rice conferring resistance to brown planthopper (bph) insect pest. | 2016 | 27834403 | |
a genome-wide identification and analysis of the basic helix-loop-helix transcription factors in brown planthopper, nilaparvata lugens. | the basic helix-loop-helix (bhlh) transcription factors in insects play essential roles in multiple developmental processes including neurogenesis, sterol metabolism, circadian rhythms, organogenesis and formation of olfactory sensory neurons. the identification and function analysis of bhlh family members of the most destructive insect pest of rice, nilaparvata lugens, may provide novel tools for pest management. here, a genome-wide survey for bhlh sequences identified 60 bhlh sequences (nlbhlh ... | 2016 | 27869716 |
driving pest insect populations: agricultural chemicals lead to an adaptive syndrome in nilaparvata lugens stål (hemiptera: delphacidae). | the brown planthopper (bph) is a devastating pest of rice throughout asia. in this paper we document the bph biogeographic range expansion in china over the 20-year period, 1992 to 2012. we posed the hypothesis that the range expansion is due to a syndrome of adaptations to the continuous presence of agricultural chemicals (insecticides and a fungicide) over the last 40 years. with respect to biogeography, bph ranges have expanded by 13% from 1992 to 1997 and by another 3% from 1997 to 2012. in ... | 2016 | 27876748 |
bph32, a novel gene encoding an unknown scr domain-containing protein, confers resistance against the brown planthopper in rice. | an urgent need exists to identify more brown planthopper (nilaparvata lugens stål, bph) resistance genes, which will allow the development of rice varieties with resistance to bph to counteract the increased incidence of this pest species. here, using bioinformatics and dna sequencing approaches, we identified a novel bph resistance gene, loc_os06g03240 (msu locus id), from the rice variety ptb33 in the interval between the markers rm19291 and rm8072 on the short arm of chromosome 6, where a gen ... | 2016 | 27876888 |
microrna and dsrna targeting chitin synthase a reveal a great potential for pest management of the hemipteran insect nilaparvata lugens. | two rna silencing pathways in insects are known to exist that are mediated by short interfering rnas (sirnas) and micrornas (mirnas), which have been hypothesised to be promising methods for insect pest control. however, a comparison between mirna and sirna in pest control is still unavailable, particularly in targeting chitin synthase gene a (chsa). | 2017 | 27885784 |
rnai-mediated resistance to rice black-streaked dwarf virus in transgenic rice. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus in the family reoviridae, causes significant economic losses in rice production in china and many other asian countries. development of resistant varieties by using conventional breeding methods is limited, as germplasm with high level of resistance to rbsdv have not yet been found. one of the most promising methods to confer resistance against rbsdv is the use of rna interference (rnai) technology. rbsdv non-structural pro ... | 2017 | 27900537 |
development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311. | brown planthopper (bph) is the most destructive pest of rice in asia. to date 29 bph resistance genes have been identified, but only a few genes are being used in breeding due to inefficient markers for marker-assisted selection (mas) and little knowledge of the real effects of the genes. in this study we individually transferred 13 genes or qtls (bph14, qbph3, qbph4, bph17, bph15, bph20, bph24, bph6, bph3, bph9, bph10, bph18 and bph21) into cultivar 9311 by marker assisted backcross breeding (m ... | 2016 | 27901104 |
resistance monitoring and cross-resistance patterns of three rice planthoppers, nilaparvata lugens, sogatella furcifera and laodelphax striatellus to dinotefuran in china. | three rice planthoppers, brown planthopper, nilaparvata lugens, white-backed planthopper, sogatella furcifera and small brown planthopper, laodelphax striatellus, are important pests of cultivated rice in tropical and temperate asia. they have caused severe economic loss and developed resistance to insecticides from most chemical classes. dinotefuran is the third neonicotinoid which possesses a broad spectrum and systemic insecticidal activity. we determined the susceptibility of dinotefuran to ... | 2016 | 27914544 |
population dynamics of rice planthoppers, nilaparvata lugens and sogatella furcifera (hemiptera, delphacidae) in central vietnam and its effects on their spring migration to china. | rice planthopper (rph) populations of nilaparvata lugens and sogatella furcifera periodically have erupted across asia. predicting rph population dynamics and identifying their source areas are crucial for the management of these migratory pests in china, but the origins of the migrants to temperate and subtropical regions in china remains unclear. in particular, their early migration to china in march and april have not yet been explored due to a lack of research data available from potential s ... | 2017 | 27919313 |
virus altered rice attractiveness to planthoppers is mediated by volatiles and related to virus titre and expression of defence and volatile-biosynthesis genes. | viruses may induce changes in plant hosts and vectors to enhance their transmission. the white-backed planthopper (wbph) and brown planthopper (bph) are vectors of southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv), respectively, which cause serious rice diseases. we herein describe the effects of srbsdv and rrsv infections on host-selection behaviour of vector and non-vector planthoppers at different disease stages. the y-tube olfactometer choice and free-choic ... | 2016 | 27924841 |
genome sizes of nine insect species determined by flow cytometry and k-mer analysis. | the flow cytometry method was used to estimate the genome sizes of nine agriculturally important insects, including two coleopterans, five hemipterans, and two hymenopterans. among which, the coleopteran lissorhoptrus oryzophilus (kuschel) had the largest genome of 981 mb. the average genome size was 504 mb, suggesting that insects have a moderate-size genome. compared with the insects in other orders, hymenopterans had small genomes, which were averagely about ~200 mb. we found that the genome ... | 2016 | 27932995 |
molecular characterization of rice oslcb2a1 gene and functional analysis of its role in insect resistance. | in plants, sphingolipids, such as long-chain bases (lcbs), act as bioactive molecules in stress responses. until now, it is still not clear if these lipids are involved in biotic stress responses to herbivore. herein we report that a rice lcb gene, oslcb2a1 encoding a subunit of serine palmitoyltransferase (spt), a key enzyme responsible for the de novo biosynthesis of sphingolipids, plays a critical role in plant defense response to the brown planthopper (bph) attack and that its up-regulation ... | 2016 | 27990147 |
insulin receptors and wing dimorphism in rice planthoppers. | wing polymorphism contributes significantly to the success of a wide variety of insects. however, its underlying molecular mechanism is less well understood. the migratory planthopper (bph), nilaparvata lugens, is one of the most extensively studied insects for wing polymorphism, due to its natural features of short- and long-winged morphs. using the bph as an example, we first surveyed the environmental cues that possibly influence wing developmental plasticity. second, we explained the molecul ... | 2017 | 27994130 |
effects of temperature on fitness costs in chlorpyrifos-resistant brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | insecticide resistance is inevitable if an insecticide is widely used to control insect pests. fortunately, the resistance-associated fitness costs often give chances to manage resistances. in most cases, the fitness cost in resistant insects is often evaluated under laboratory conditions for insect development, which limits its practical application in pest control in the field. in a laboratory population r9 with 253-fold resistance to chlorpyrifos after nine-generation selection with chlorpyri ... | 2016 | 28026125 |
pancreatic lipase-related protein 2 is essential for egg hatching in the brown planthopper, nilaparvata lugens. | in this study, we identified a pancreatic lipase-related protein 2 (plrp2) gene through searching the transcriptome data of the brown planthopper, nilaparvata lugens, a monophagous rice pest. plrp2 mrnas were first isolated in the human pancreas and play an important role in hydrolysis of galactolipids from the diet. although homologous plrp2 genes have been identified in many insect species, their physiological functions remain unknown. the present study for the first time reports the functiona ... | 2017 | 28032922 |
a mitochondrial membrane protein is a target for rice ragged stunt virus in its insect vector. | rice ragged stunt virus (rrsv; reoviridae) is exclusively transmitted by the brown planthopper nilaparvata lugens in a persistent-propagative manner. it is understood that rna viral proliferation is associated with the intracellular membranes of the insect host cells. however, the molecular mechanisms of the interaction between the rrsv proliferation and the intracellular membranes remain essentially unknown. it will be of great interest to determine whether rrsv protein(s) directly interact wit ... | 2017 | 28034779 |
effect of rnai-mediated knockdown of nltor gene on fertility of male nilaparvata lugens. | the target gene of rapamycin (tor) is conserved from invertebrates to vertebrates, and plays critical roles in cell growth, nutrient sensing, lifespan and reproduction. in this paper, we employed rna interference (rnai) to study the function of tor in male brown planthoppers (bph), nilaparvata lugens. here we discovered that no offspring was produced when wildtype females bph mated with nltor rnai males. to understand the influence of nltor on male bph infertility, we dissected the reproductive ... | 2017 | 28069465 |
induction of p450 genes in nilaparvata lugens and sogatella furcifera by two neonicotinoid insecticides. | nilaparvata lugens and sogatella furcifera are two primary planthoppers on rice throughout asian countries and areas. neonicotinoid insecticides, such as imidacloprid (imi), have been extensively used to control rice planthoppers and imi resistance consequently occurred with an important mechanism from the over-expression of p450 genes. the induction of p450 genes by imi may increase the ability to metabolize this insecticide in planthoppers and increase the resistance risk. in this study, the i ... | 2017 | 28092127 |
a salivary ef-hand calcium-binding protein of the brown planthopper nilaparvata lugens functions as an effector for defense responses in rice. | the brown planthopper (bph), nilaparvata lugens (stål) (hemiptera: delphacidae), a major pest of rice in asia, is able to successfully puncture sieve tubes in rice with its piercing stylet and then to ingest phloem sap. how bph manages to continuously feed on rice remains unclear. here, we cloned the gene nlsef1, which is highly expressed in the salivary glands of bph. the nlsef1 protein has ef-hand ca2+-binding activity and can be secreted into rice plants when bph feed. infestation of rice by ... | 2017 | 28098179 |
design, synthesis, and bioactivities of novel oxadiazole-substituted pyrazole oximes. | a series of novel pyrazole oxime derivatives containing a substituted oxadiazole group were designed and synthesized. the bioassay results indicated that some title compounds displayed good acaricidal and insecticidal activities against tetranychus cinnabarinus, aphis medicaginis, oriental armyworm, and nilaparvata lugens. especially, compounds 7a, 7b, and 7c had 80%, 90%, and 90% insecticidal activities against a. medicaginis at 20μg/ml, respectively. interestingly, some of the designed compoun ... | 2017 | 28108247 |
transcriptome analysis reveals the response of iron homeostasis to early feeding by small brown planthopper in rice. | it has been documented that planthopper attacks change iron (fe) content of rice plants. to investigate whether planthopper attacks change rice fe homeostasis at the molecular level, the response of rice fe homeostasis to early feeding by small brown planthopper (sbph) was examined by transcriptome profiling. results showed that the concentration of fe and nicotianamine decreased in resistant rice genotype and increased in susceptible rice genotype after attack by sbph. transcriptome profiling s ... | 2017 | 28112511 |
mucin-like protein, a saliva component involved in brown planthopper virulence and host adaptation. | the rice brown planthopper (bph), nilaparvata lugens, can rapidly adapt to new resistant rice varieties within several generations, rendering its management burdensome. however, the molecular mechanism underlying its adaptability remains unclear. in this study, we investigated the potential role of mucin-like protein (nlmul) in n. lugens virulence and adaptation to host resistance. nlmul is an important glycoprotein that constitutes both gelling and watery saliva, and specifically expressed in t ... | 2017 | 28115117 |