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the influence of thiourea on the development of the cultured quail embryo.although thyroid hormones are known to promote embryonic development, it is still questionable whether the hormones of the thyroid gland exert a stimulatory effect on the early embryo. in attempting to elucidate the thyroid function in this period, we tried to block the synthesis of thyroid hormones by thiourea and followed the embryogenesis under these conditions. the experiments were performed with cultured quail embryos (coturnix coturnix japonica), which seemed especially suitable for those ...19911905865
estrogen target cells during the early stage of medullary bone osteogenesis: immunohistochemical detection of estrogen receptors in osteogenic cells of estrogen-treated male japanese quail.the localization of estrogen receptors (er) in osteogenic cells during the early stage of medullary bone osteogenesis was studied immunohistochemically in the femurs of estrogen-treated male japanese quail. alkaline phosphatase (alp) activity was used as a marker for osteogenic cells. er immunostaining was observed in the nuclei of weak alp-positive bone lining cells on the endosteal bone surface of nontreated birds. after 24 hours of estrogen treatment, nuclear immunostaining was detected in al ...19911913292
production of transgenic birds.the avian embryo presents a tremendous challenge for those interested in accessing and manipulating the avian germ line. by far the most successful method of gene transfer is by retrovirus vector. the efficacy of retrovirus vectors has been demonstrated by germ line insertion of replication-competent retroviruses as well as the insertion of replication-defective retrovirus vectors carrying bacterial marker genes. retroviral vectors have also been shown to be useful for the transfer and expressio ...19911915773
genetic and phenotypic relationships in japanese quail. 1. body weight, carcass, and organ measurements.live bw, carcass data, and organ data taken at 34 days of age on approximately 1,000 quail of both sexes from 110 sires and 290 dams were utilized to estimate genetic parameters from the initial generation of a selection study. the birds represented a sample of a line selected for high 4-wk bw. data were analyzed using henderson's method 3 (mm3) and restricted maximum likelihood (reml) with and without a relationship matrix. the paternal half-sib heritabilities for body, carcass, and organ weigh ...19911924086
research note: effects of dietary fats on hepatic cholesterol synthesis in japanese quail.blood lipid concentrations and hepatic cholesterol synthesis were compared in japanese quail fed diets containing fats with different fatty acid profiles. the quail fed a diet containing tuna oil had the lowest blood cholesterol concentration; those fed beef drippings the highest, and those fed safflower oil or linseed oil had intermediate concentrations. rates of hepatic cholesterol synthesis mirrored the results for serum cholesterol concentration. serum triglyceride concentrations were lower ...19911924102
thermal and metabolic responsiveness of japanese quail embryos following periodic exposure to 2,450 mhz microwaves.two studies were performed to determine if repeated exposure of the avian egg to microwaves can alter metabolism, temperature, and growth rate of embryos. another aim was to supplement conventional heating with microwave heating and provide an optimal temperature for growth. japanese quail (coturnix coturnix japonica) eggs were exposed from day 1 through 15 of incubation (8 h/day) to sham or microwave (2,450 mhz) irradiation. microwave exposures were at two power densities, 5 or 20 mw/cm2, and a ...19911930307
localization of neuropeptide y-immunoreactive cells and fibres in the brain of the japanese quail.in the present study, we have demonstrated, by means of the biotin-avidin method, the widespread distribution of neuropeptide y (npy)-immunoreactive structures throughout the whole brain of the japanese quail (coturnix coturnix japonica). the prosencephalic region contained the highest concentration of both npy-containing fibres and perikarya. immunoreactive fibres were observed throughout, particularly within the paraolfactory lobe, the lateral septum, the nucleus taeniae, the preoptic area, th ...19911934027
immunoelectron microscopic demonstration of estrogen receptors in osteogenic cells of japanese quail.the localization of estrogen receptors (ers) in osteogenic cells was immunoelectron microscopically examined in the femurs of female and estrogen-treated male japanese quail. an electron dense reaction product showing er localization was observed in the nuclei of osteoblasts and immature osteocytes in the medullary bone of the female quail. however, reaction product was not seen in the osteoclasts. on the endosteal bone surface of male quail, nuclear reaction product was detected in bone lining ...19911938479
purification and characterization of neutral alpha-mannosidase that is activated by co2+ from japanese quail oviduct.an alpha-mannosidase was purified from the magnum section of japanese quail oviduct by ammonium sulfate precipitation, deae-sephacel chromatography, sephacryl s-300 chromatography, mannan-sepharose 4b chromatography, and hydroxyapatite chromatography. the purified alpha-mannosidase (referred to as neutral alpha-mannosidase) showed a single band on polyacrylamide gel with or without sodium dodecyl sulfate. its molecular weight was found to be 330,000 by gel chromatography. neutral alpha-mannosida ...19911939023
experimental infection of japanese quail with toxocara canis larvae through earthworms.the migration and distribution of toxocara canis larvae in the tissues of earthworms exposed to 5 x 10(5) infective eggs in soil, as well as the reinfectivity of the larvae in japanese quail fed with three earthworms, were studied. the average number of larvae recovered from an earthworm gradually declined from 9 +/- 3.21 to 4.5 +/- 2.00 at 4-16 days. no larvae were recovered at day 20. when compared between three portions of the earthworms' body, the middle part showed the greatest recovery in ...19911957494
japanese quail and human acid maltase deficiency: a comparative study.a morphological study was carried out on the skeletal muscle of a mutant japanese quail with acid maltase deficiency (amd). the affected quails began to experience difficulty in lifting their wings about 6 weeks after hatching. four weeks after hatching, before symptoms appeared, alpha-1, 4-glucosidase activity in skeletal muscle was decreased to less than 10% of the control level, and muscle fibers possessed many vacuoles containing periodic acid schiff (pas) positive material which was digeste ...19911957974
testosterone metabolism in the avian hypothalamus.many central actions of testosterone (t) require the transformation of t into several metabolites including 5 alpha-dihydrotestosterone (5 alpha-dht) and estradiol (e2). in birds as in mammals, 5 alpha-dht and e2, alone or in combination, mimic most behavioral effects of t. the avian brain is, in addition, able to transform t into 5 beta-dht, a metabolite which seems to be devoid of any behavioral or physiological effects, at least in the context of reproduction. by in vitro product-formation as ...19911958558
the influence of stress treatment on femur cortical bone porosity and medullary bone status in japanese quail selected for high and low blood corticosterone response to stress.1. femurs taken from japanese quail (coturnix coturnix japonica) genetically selected for either exaggerated (hs; high-stress) or reduced (ls; low-stress) blood corticosterone (b) response to unfamiliar environments were examined to determine their cortical bone porosity (cbp; degree of osteoporosis) and medullary bone (mb) status following treatment with or without stress. 2. femur cbp was elevated in both sexes of stressed, hs-line quail and was attributed directly to the presumably exaggerate ...19901970526
nonmammalian vertebrate models in studies of brain-steroid interactions.estrogen formation in brain and pituitary mediates certain androgen actions in central targets. goldfish (carassius auratus) and quail (coturnix coturnix japonica) have been advantageous for studying the role of locally formed estrogen in autoregulating aromatization and in controlling estrogen receptor occupancy, androgen receptor levels, and behavioral expression. data from these two experimental models reveal a molecular basis for androgen-estrogen synergism in neuroendocrine tissues and for ...19901974804
aggressive behavior in birds: an experimental model for studies of brain-steroid interactions.1. although testosterone (t) stimulates aggressiveness in males of many vertebrate species, it is now known that the full expression of t actions in the central nervous system requires aromatization to estradiol (e2) and subsequent binding of formed e2 to its receptor. 2. we have recently confirmed these as rate-limiting steps in the control of sex-related and individual differences in aggressiveness of the japanese quail (coturnix coturnix japonica). 3. in this review, we describe some of the n ...19901979529
serum chemistries of coturnix coturnix japonica given dietary manganese oxide (mn3o4).1. plasma creatinine and inorganic phosphorus were increased in manganese oxide (mn3o4)-treated adult male coturnix quail, but bun, bun/creatinine ratio, uric acid, and total calcium were decreased. 2. serum enzymes (alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, and lactic dehydrogenase) were elevated in mn3o4-treated adult male coturnix quail, but creatine phosphokinase was not affected. 3. dietary mn3o4 at 5000 ppm did not produce overt signs of toxico ...19901981341
photoperiodic control of the termination of reproduction in japanese quail (coturnix coturnix japonica).typically, birds come into breeding in the spring as a response to long days, and end reproduction some weeks later by becoming refractory to those long days. the refractory state is subsequently dissipated by the short days of autumn and winter, so producing once again a bird that can respond to long days. bird species differ in the extent to which refractoriness is developed; the present experiments took advantage of the relative, rather than the absolute, refractoriness in quail to measure qu ...19901983038
structural and immunohistochemical aspects of the postovulatory follicle in japanese quail.in the present study, we searched for the presence of granulosa lipid spheres, of lacunae, and of the smooth-muscle markers desmin and alpha-smooth-muscle actin, in the wall of the pof1 of the quail ovary. lacunae, using the labelled yolk technique, were visible as large cavities in the pof1 wall. the immunohistochemical localization of desmin was similar to that observed in preovulatory follicles. a similar distribution was observed using an anti-alpha-smooth-muscle actin antiserum, but the cel ...19911996782
perpetuation of muscle fibers after removal of stretch in the japanese quail.stretch-overload has been shown to increase muscle mass in the anterior latissimus dorsi (ald) of the adult quail by increasing both fiber size and number, but it is not known whether the new muscle fibers or fiber size is maintained after removal of the stretch stimulus. a weight was added to the right wing of 40 adult quail while the left wing in each bird served as an intra-animal control. the weight was removed after 30 days of stretch, and terminal experiments were conducted 0, 30, 60, or 9 ...19912003569
structural and biochemical effects in lungs of japanese quail following a 1-week exposure to ozone.the effect of ozone inhalation on birds was investigated. japanese quail were exposed continuously to 0, 0.3, 1.0, and 3.0 mg/m3 ozone (0, 0.15, 0.50, and 1.50 ppm, respectively) for 7 days. pulmonary effects were determined by light and electron microscopy as well as by biochemistry. focal areas of hemorrhages were noticed in the birds exposed to 1.0 mg/m3 ozone. additional effects after exposure to 1 mg/m3 included loss of cilia in trachea and bronchi, an inflammatory response, and necrosis of ...19912004636
the serotoninergic system in the brain of the japanese quail. an immunohistochemical study.the presence and topographical localization of the serotoninergic system in the brain of the japanese quail (coturnix coturnix japonica) have been studied by means of peroxidase-anti-peroxidase immunocytochemistry. the perimeter, diameter, area, and shape factor of immunoreactive cells have been recorded and analyzed morphometrically for intra- and interspecies comparison. the data reported here confirm and extend results previously obtained in the brain of other avian species. serotonin-immunor ...19912007251
effects of testosterone on a selected neuronal population within the preoptic sexually dimorphic nucleus of the japanese quail.the effects of testosterone on the volume and cytoarchitecture of the sexually dimorphic nucleus of the preoptic area (pom) were investigated in male and female japanese quail. it was confirmed that castration decreases the pom volume in males and that, in gonadectomized birds of both sexes, testosterone increases this volume to values similar to those observed in intact sexually mature males. this suggests that the sex difference in pom volume results from a differential activation by t so that ...19912007660
metabolism of narasin in chickens and japanese quail.thirty mature chicken hens and 60 mature japanese quail hens were used to compare pathways of narasin excretion. carbon-14-labeled narasin was injected into chickens (.7 microci) and quail (.113 microci) via cardiac puncture. blood, sampled at varying times thereafter, and eggs and excreta collected daily for 28 days, were analyzed for 14c. groups of six chickens and 12 quail were killed prior to [14c]narasin injection and on days 1, 7, 14, and 28 postinjection to obtain tissue samples for 14c a ...19912017406
hepatic 5'-deiodinase activity of japanese quail using reverse-t3 as substrate: assay validation, characterization, and developmental studies.using rt3 as substrate, an in vitro 5'd assay was validated for use with liver tissue from adult japanese quail, by defining conditions under which activity is proportional to enzyme (protein) concentration and is linear with incubation time. activity was measured as the release of 125i from labeled rt3. using validated assay conditions we found the following 5'd characteristics: maximal activity from 10 to 50 mm dithiothreitol (cofactor), an apparent km of 0.52 microm rt3, ph optimum of 7.6-8.5 ...19912022947
parallel measurement of brain acetylcholinesterase and the muscarinic cholinergic receptor in the diagnosis of acute, lethal poisoning by anti-cholinesterase pesticides.the activity of acetylcholinesterase (ache) and the density of muscarinic cholinergic binding receptors (mcbr) were measured in brains from normal japanese quail (coturnix coturnix japonica) and from quail after lethal intoxication with diazinon. these were measured in brains from whole heads held at 25 c for 0 to 8 days after death. the maximum relative loss of activity due to post mortem decomposition alone during 8 days was 13% and 10% for ache and mcbr, respectively. during post mortem decom ...19912023308
neuroanatomical specificity in the co-localization of aromatase and estrogen receptors.the relative distributions of aromatase and of estrogen receptors were studied in the brain of the japanese quail by a double-label immunocytochemical technique. aromatase immunoreactive cells (aro-ir) were found in the medial preoptic nucleus, in the septal region, and in a large cell cluster extending from the dorso-lateral aspect of the ventromedial nucleus of the hypothalamus to the tuber at the level of the nucleus inferioris hypothalami. immunoreactive estrogen receptors (er) were also fou ...19912030339
genetic and experiential manipulation of fear-related behavior in japanese quail chicks (coturnix coturnix japonica).we examined effects of environmental enrichment and regular handling on fear-related responses in chicks of japanese quail (coturnix coturnix japonica) selected over several generations for short or long tonic immobility (sti and lti, respectively). sti chicks froze less, vocalized and walked sooner and more in an open field, emerged sooner from a hole-in-the-wall box, and showed shorter ti reactions than did chicks of the lti line. similarly, environmental enrichment reduced silence and inactiv ...19912032451
divergent selection for duration of tonic immobility and social reinstatement behavior in japanese quail (coturnix coturnix japonica) chicks.japanese quail (coturnix coturnix japonica) were selected over 8 generations for long or short duration of tonic immobility (ti) weighted for independence from social reinstatement (sr) behavior (as measured in a treadmill) and for high or low levels of sr behavior weighted for independence from duration of ti. divergent selection for long or short duration of ti and for high or low levels of sr behavior resulted in immediate and significant responses that continued throughout the 8 generations. ...19912032452
relative salience of species maternal calls in neonatal gallinaceous birds: a direct comparison of japanese quail (coturnix coturnix japonica) and domestic chickens (gallus gallus domesticus).differential-approach tendencies of individual incubator-hatched chickens and japanese quail were assayed using one exemplar of each of the species maternal calls in a simultaneous-choice paradigm. within 24 hr after hatching, the birds received several short periods of exposure to rotating mounts of an adult female chicken and quail, which emitted the species-appropriate call. all birds were then tested with calls alone over the following 2 to 4 days. the test stimuli were played from speakers ...19912032455
transcription of a quail gene expressed in embryonic retinal cells is shut off sharply at hatching.the avian neuroretina (nr) is part of the central nervous system and is composed of photoreceptors, neuronal cells, and müller (glial) cells. these cells are derived from proliferating neuroectodermal precursors that differentiate after terminal mitosis and become organized in cell strata. genes that are specifically expressed at the various stages of retinal development are presently unknown. we have isolated a quail (coturnix coturnix japonica) cdna clone, named qr1, encoding a 676-amino acid ...19912034690
development of the glycogen body of the japanese quail, coturnix japonica: ii. observations of electron microscopy.transmission electron microscopic observations of the relationships of the cells of the glycogen body and those of nervous tissue in the lumbosacral spinal cord show that one day after hatching, glycogen cells at the lateral margins of the glycogen body lie in close association with elements of the neuropil in the adjacent spinal cord. glycogen cells and their processes appear to extend into the neuropil, where they contact other glycogen cells, blood vessels, neurons, and neuroglia. junctional ...19912038064
[ultrastructural and immunohistochemical study of experimental atherosclerosis in japanese quails].a marked plaque was produced at the tunica intima of the ascending aorta in all of the japanese quails of 9 weeks old which fed on atherogenic diet containing 2% cholesterol for 8 weeks, while no structural changes of aortic wall were observed in japanese quails which fed on normal basic food for the same period. the media of aorta in normal quails consist of smooth muscle cells, myofibroblast-like cells (mf) cells), and many successive elastic membranes. at the atherosclerotic lesion, many mf c ...19912044665
effects of surgical deafening and exposure to continuous darkness on cloacal gland size in scotorefractory japanese quail.adult male quail were transferred to short days (sd) and exposed simultaneously to conspecific vocalizations of males on long days (cvmld). cloacal glands did not regress in some males while in others their glands regressed then spontaneously recrudesced. of the birds that exhibited cloacal gland regression, 80% recrudesced by 8 weeks and all birds recrudesced by 25 weeks on sd. three experiments are reported. the first experiment analyzed the effects of transfer to sd. the remaining two experim ...19912045093
divergent selection for growth in japanese quail under split and complete nutritional environments. 4. genetic and correlated responses from generations 12 to 20.divergent selection during generations 12 to 20 for high (h) and low (l) 4-wk bw under split-(sd) and complete-(cd) diet environments resulted in continued progress in both upward and downward directions. following 20 generations of selection, there were 4.8- and 3.6-fold differences between bw of h and l lines in the sd and cd environments, respectively. regression coefficients of mean 4-wk bw on generation of 1.88 and 1.58 g in the h lines and -1.50 and -1.37 g in the l lines indicated symmetr ...19912047338
effects of selection on plasma thyroxine concentrations in japanese quail under thiouracil and protein stress.three experiments were conducted to measure changes in plasma thyroxine (t4) concentrations occurring in male and female japanese quail in response to treatment with dietary thiouracil (tu) and different cp levels prior to sexual maturity and to determine the influence of selection for growth under tu and protein stress on this response. selected and unselected lines of quail were fed diets containing .2% tu or two levels of cp (20 or 28%) or both from 0 to 4 wk of age. body weight and plasma t4 ...19912047354
an endocrine response to short-term hypodynamy in japanese quail selected for resistance to hypodynamy. 19912047409
the effect of the different gravity on the muscle composition in japanese quail. 19912047410
embryonic development of japanese quail under microgravity conditions. 19912047468
maturation-promoting factor and p34cdc2 kinase during oocyte maturation of the japanese quail.maturation-promoting factor and a homolog of fission yeast cdc2+ gene product (p34cdc2) were investigated during the final 24 hr of maturation of quail oocytes. kinase activity of p34cdc2 in the oocyte germinal disk (gd) increased 15 times at maturation. two bands, at 32 and 34 kda, were detected in immature oocytes by immunoblotting of sds-page with anti-p34cdc2 monoclonal antibody. a new band, which is close to the 32-kda band but with a slightly faster mobility, appeared during maturation. no ...19912060707
nocturnal hypothermia in fasting japanese quail: the effect of ambient temperature.japanese quail were equipped with intraperitoneal transmitters for telemetric measurement of body temperature (tb) and activity. food deprivation at + 24 degrees c for four days induced a well-defined nocturnal hypothermic response. the normal day-night difference (about 1 degrees c) in tb increased 1 degrees c per day, reaching 5 degrees c on the fourth night of fast. nocturnal motor activity decreased during the fast, while daytime activity first increased and then returned to the original lev ...19912062935
androgen and estrogen action in the preoptic area and activation of copulatory behavior in quail.the sites of androgen and estrogen action on sexual behavior were studied in the preoptic area of castrated male japanese quail by stereotaxic implantation of hormones, antihormones and metabolism inhibitors. the first experiment demonstrated that bilateral implantation of the aromatase inhibitor, androstatrienedione (atd), in the sexually dimorphic nucleus (pom) of the preoptic area can completely suppress the behavioral activation produced by a systemic treatment with testosterone. the effects ...19902082358
hatching of japanese quail chicks (coturnix coturnix japonica) following long, daily cyclical interruptions of their incubation.the adaptive advantages were studied of long, daily cyclical interruptions of incubation for the hatching of japanese quail. the aim was to mimic in a more pronounced form the natural cyclical variations to which avian embryos may be subjected and still survive. the results indicated that by consecutive daily 8-h interruptions of incubation it was possible to lengthen the embryonic period by one-third without apparent harm and with potential improvement in hatchability. after consecutive daily 1 ...19902084682
structural difference of chymotrypsinogens forming chymotrypsin variants in japanese quail.a chymotrypsinogen showing the phenotype aa of chymotrypsin was purified from quail pancreas, and its chromatographic behavior, molecular weight, and electrophoretic mobility were very similar to those of another chymotrypsinogen, showing the aa phenotype of chymotrypsin. however, after activation, this chymotrypsinogen from aa showed band 5 of chymotrypsin, while the other chymotrypsinogen from aa did not. peptide mapping demonstrated that the molecular structures of the two chymotrypsinogens a ...19902085308
purification and characterization of chymotrypsinogen from pancreas of japanese quail (coturnix coturnix japonica).1. a chymotrypsinogen from pancreas of japanese quail (coturnix coturnix japonica) was purified by acid extraction, salt fractionation and chromatographic separation on cm-cellulose and sephadex g-100, and gave a single protein band on sds-page. 2. quail chymotrypsinogen had a mol. wt of 26,100 calculated from amino acid composition data, an isoelectric point of 7.68, a km of 3.1 mm and k0 of 40.7 sec-1 for tyrosine ester substrate. 3. the activated chymotrypsinogen of quail had a maximum activi ...19902085958
characterization of insulin receptors in primary cultures of quail (coturnix coturnix japonica) oviduct cells. the level of insulin receptor is regulated by steroid and peptide hormones.1. we have characterized the insulin receptor in primary cultured quail oviduct cells and examined the hormonal regulation of its level. 2. we have also shown the recycling pathway of insulin receptors in the cultured cells using specific inhibitors (tunicamycin, chloroquine, monensin, and brefeldin a). 3. our data suggest that glucocorticoids play important physiological roles in egg-white protein synthesis through increasing the number of insulin receptors and insulin through enhancing the tra ...19902085960
toxicological and biological studies on japanese quails fed graded levels of furazolidone.furazolidone (fz) was administered to 42-day-old female japanese quails as a feed additive at doses of 0, 200, 400, 600 and 800 ppm for a period of 28 days. dose-dependent effects were observed. high levels of fz (600 and 800 ppm) significantly altered growth, decreased feed consumption, caused marked atrophy of the ovaries and oviducts leading to cessation of egg laying, and resulted in higher mortality. hepatotoxicity was evidenced by an increase in serum aspartate aminotransferase (ast), alan ...19902099613
interaction between eimeria uzura infection and aflatoxicosis in japanese quail (coturnix coturnix japonica).the clinical signs and gross lesions caused by eimeria uzura (10(5) oocysts) in japanese quail (coturnix coturnix japonica) exhibited little or no influence in the face of intercurrent dietary aflatoxicosis (1 p.p.m. of aflatoxin b1 from day 0 to 55). similarly, no significant differences in the mucosal morphology of the intestine were evident histologically between the two groups of japanese quail. the nervous signs of ataxia, leg weakness, incoordination of movement, torticollis and terminal o ...19902111599
the influence of supplemental corn oil and free fatty acids on the reproductive performance of japanese quail (coturnix coturnix japonica).an experiment was conducted to evaluate the effects of dietary corn oil (co) and commercial free fatty acids on the fatty acid composition of blood plasma and egg yolk lipids and on the reproductive performance of japanese quail. when quail were fed a semi-purified low-fat basal mix, the substitution of 3% of co for corn starch (cs) improved egg weight (11.2 versus 10.8 g), reduced late embryonic mortality (16.7 versus 28.4%), and improved hatchability (75.7 versus 64.1%). diets in which palmiti ...19902123342
renal carbonic anhydrase in the quail coturnix coturnix japonica: i. activity and distribution in male and female metanephros.carbonic anhydrase activity was studied in the quail metanephros by means of histochemical, histophotometrical and biochemical methods. male and female samples were examined separately in order to show sex-related differences in enzyme activity and localization. the staining patterns revealed differential distribution of reaction product in the different tubular segments. the initial portion of proximal tubules showed positivity on the brush border in female kidneys only. extra situ investigatio ...19902127415
ultradian and circadian compartmentalization of respiratory and metabolic exchanges in small laboratory vertebrates.carbon dioxide emission (vco2) taken as an index of respiratory and metabolic exchanges, was continuously recorded during 4-30 consecutive days in 100 quail, 87 chicks, 347 rats, 665 mice and 70 guinea-pigs which were under controlled environmental parameters. harmonic analysis, fast fourier transform, chi-square periodograms, peak and trough intervals were computed with vco2 values obtained with co2 concentrations sampled every 20 min on the co2 recordings. in ld 12:12 alternation, circadian rh ...19902128229
the quail's eye: a biological clock.the site (intraocular vs. extraocular) of the biological clock driving a rhythm in melatonin content in the eyes of japanese quail was investigated by alternately patching the left and right eyes of individual birds, otherwise held in constant light, for 12-hr periods. this patching protocol, therefore, exposed each eye to a light-dark cycle (ld 12:12) 180 degrees (12 hr) out of phase with the ld cycle experienced by the other eye. the optic nerves to both eyes were transected prior to initiatin ...19902133136
melatonin does not link the eyes to the rest of the circadian system in quail: a neural pathway is involved.blinding by enucleation has a dramatic effect on the circadian activity rhythm of japanese quail. the activity patterns of enucleated birds held under 24-hr light-dark cycles are disrupted, although entrainment can persist in many birds. in constant darkness (dd), blinded birds are rendered arrhythmic. these results demonstrate that the eyes are a major component of the circadian system, and that insofar as enucleation produces arrhythmicity in dd, the eyes' role is not merely a photosensory one ...19902133141
muscle fiber formation and fiber hypertrophy during the onset of stretch-overload.the contributions of fiber hypertrophy and new fiber formation to the onset of stretch-induced muscle enlargement were evaluated in the anterior latissimus dorsi (ald) of adult japanese quails, because it was not known whether the mechanisms which initiate new fiber formation were dependent on first achieving significant fiber hypertrophy. a weight corresponding to 10% of the bird's body mass was attached to one wing, and eight birds were killed after each day during the first week of stretch. m ...19902142581
glycyl-l-glutamine stimulates the accumulation of a12 acetylcholinesterase but not of nicotinic acetylcholine receptors in quail embryonic myotubes by a cyclic amp-independent mechanism.myotubes prepared from the japanese quail embryo at 9 days gestation were cultivated in the presence of glycyl-l-glutamine (gly-gln, beta-endorphin c-terminal dipeptide) or glycyl-glutamic acid (gly-glu), and changes in the activity of acetylcholinesterase (ache) molecular forms and binding of 125i-alpha-bungarotoxin (alpha bgt) to cell surface nicotinic acetylcholine receptors were measured. the a12 oligomer was the major form of ache in the cultures. the activity of all molecular forms of the ...19902156012
plasma cholesterol responses to the adrenocorticotropic hormone in japanese quail following eighteen generations of divergent selection.japanese quail (coturnix coturnix japonica) lines were divergently selected for plasma cholesterol response to adrenocorticotropin (acth). data for generations 9 to 18 indicated a greater response in the low (l) line than in the high (h) line, which was contrary to selection responses for the first eight generations. during generations 9 to 18, selection differentials were greater in the h line; however, heritability estimates were greater in the l line. these data suggest caution in the interpr ...19902158649
diagnostic exercise: wasting syndrome in a flock of japanese quail. 19902166871
vasopressor and depressor effects of native angiotensins and inhibition of these effects in the japanese quail.the amino acid sequence of angiotensin i (ang i) from the japanese quail, coturnix coturnix japonica, obtained from incubation of homologous plasma and kidney extract was determined as h-asp-arg-val-tyr-val-his-pro-phe-ser-leu-oh. a bolus, intravenous injection of native ang i or of ang ii induced an immediate vasodepressor response and a subsequent vasopressor response in quail which has been lightly anesthetized with urethane (0.75 g/kg). the values for ed50 for the vasopressor and depressor e ...19902191893
aromatization mediates aggressive behavior in quail.although testosterone (t) stimulates aggressive and reproductive behaviors in males of many vertebrate species, it is now known that the full expression of t action in the brain requires aromatization to estradiol (e2) and subsequent interaction of locally formed e2 with nuclear estrogen receptors. in experiments reported here, we used a behavioral test which quantifies the response of an individual male japanese quail (coturnix coturnix japonica) to the visual stimulus of a conspecific. we have ...19902191894
production of avian antibodies to three potyviruses in coturnix quail.avian antibodies against three potyviruses were produced in a small bird, coturnix quail (coturnix coturnix japonica temminck et schlegel), with 15-60 micrograms of purified virus preparations. intramuscular injections of immunogen with freund's incomplete or complete adjuvant into the birds did not result in higher titer of antibody compared to that of control birds given intravenous injections. quail egg yolk antibody was as useful as hen antibody for indirect-elisa and allowed virus to be det ...19902196273
the sequence of japanese quail ovalbumin cdna. 19902216734
muscarinic cholinergic receptors in the songbird and quail brain: a quantitative autoradiographic study.in order to clarify the neuroanatomical basis for postulated muscarinic cholinergic control of a wide array of physiological processes in birds, the distribution of muscarinic cholinergic receptors in the brain of three avian species was investigated by quantitative autoradiography. the species consisted of two passerines (songbirds), the european starling (sturnus vulgaris) and the song sparrow (melospiza melodia), and one galliform, the japanese quail (coturnix coturnix japonica). [3h]n-methyl ...19902229474
tissue deposition and passage into eggs of ochratoxin a in japanese quail.laying japanese quail (coturnix coturnix japonica) were given single oral doses of 0, 1, 5, and 20 mg of ochratoxin a (oa) per kg of the body weight. after 6, 12, and 24 hours and 2, 3, 4, 5, 6, and 7 days an appropriate number of birds was sacrificed and oa in blood, muscles, liver, kidney, abdominal yolks, and collected eggs was measured. the highest concentration of oa was found in kidneys and the lowest in muscles. four days following the application, oa could still be detected in kidney, li ...20122231319
divergent selection for body weight and yolk precursor in coturnix coturnix japonica. 8. a summary of correlated responses.correlated response data accompanying 19 generations of selection for increased (hw) or decreased (lw) 4-wk bw and increased (hp) or decreased (lp) total plasma p were summarized. similarly, 11 generations of selection of sublines derived from the original lines hw and lp were also analyzed. sublines were maintained through selection of males for increased 4-wk bw and females for the respective high or low plasma p. the randombred control (rbc1) was stable for bw early in egg production, bw chan ...20072235825
head skeletal abnormalities associated with the throat tuft mutation (hfdtt) in the japanese quail.hereditary abnormalities of head skeleton ascribed to embryonic visceral arch defects were investigated in japanese quail homozygous for the throat tuft mutation (hfdtt). differential staining for bone and cartilage of 15-day embryos revealed partial deletion or irregularity in the mandible, basiparasphenoid, hyoid apparatus, and quadratojugal bones, incidences of which were 84, 44, 40, and 18%, respectively. incidence of the head-skeleton abnormality was 88% when individuals having at least one ...19902235840
[the subacute and subchronic toxicity of chlorophacinone in japanese quail (coturnix c. japonica)].accordingly to the subacute toxicity of chlorphacinone to japanese quail (lc 50: 60 mg/kg feed), the uptake of delicia-chlorphacinone-baits for several days can be lethal to birds as a matter of principle. a subchronic study resulted in a "no-observed-effect-level" of 1 mg chlorphacinon/kg feed. reproduction is not affected until dose levels which resulted in lethality or are accompanied by physiological changes (coagulability of blood, microcytic anaemia). increased sensibility of descendants t ...19902241472
high-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution.the three-dimensional structure of a 30-residue synthetic peptide containing the carboxy-terminal "zinc finger" motif of a human enhancer binding protein has been determined by two-dimensional nuclear magnetic resonance (2d nmr) spectroscopy and hybrid distance geometry-dynamical simulated annealing calculations. the structure determination is based on 487 approximate interproton distance and 63 torsion angle (phi, psi, and chi 1) restraints. a total of 40 simulated annealing structures were cal ...19902248949
behavior of rabbit dental tissues in heterospecific association with embryonic quail ectoderm.the behavior of dental tissues from the rabbit, oryctolagus cuniculus, in association with epithelium from the quail, coturnix coturnix japonica, has been examined. adult and embryo rabbits were employed in this study. dental papillae from teeth at the cap stage from rabbit embryos and dental pulp from adult rabbits were isolated surgically and recombined with skin ectoderm from 72-hour-old quail embryos. the recombined tissues were cultured for 48 hours on semi-solid medium and subsequently rem ...19902250161
spatial arrangement of the stages of the cycle of the seminiferous epithelium in the japanese quail, coturnix coturnix japonica.the spatial arrangement of the stages of the cycle of the seminiferous epithelium of the japanese quail was investigated by preparing three-dimensional reconstructions of a seminiferous tubule from each of 3 quails. it was found that the stages were not distributed at random, but were arranged in a wave which spiralled helically along a seminiferous tubule. adjacent stages in space were always adjacent numbers in the cycle of the seminiferous epithelium. complete spermatogenetic waves were found ...19902250235
myosin isozyme expression in response to stretch-induced hypertrophy in the japanese quail.when skeletal muscle is subjected to stretch it undergoes a rapid increase in muscle mass. however, the effect of stretch on the native myosin isozyme content of muscle has received attention only recently. using the japanese quail to investigate stretch-induced hypertrophy, we demonstrated an increase in the expression of fast myosin in the predominantly slow anterior latissimus dorsi muscle (ald). the fast myosin content of the control quail ald is not sufficient to be quantified on native myo ...19902260780
distribution of aromatase in the brain of the japanese quail, ring dove, and zebra finch: an immunocytochemical study.an immunocytochemical peroxidase-antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase-containing cells in the brain of three avian species: the japanese quail, the ring dove, and the zebra finch. in quail and dove, immunoreactive cells were found only in the preoptic area and hypothalamus, with a high density of positive cells being present in the medial preoptic area, in the septal area above the anterior commissur ...19902262592
abdominal fat and testes weights in diverse genetic lines of japanese quail.six experiments were conducted to investigate abdominal fat levels in mature male and female japanese quail following selection for 4-wk body weight and to investigate the relationship between testes development and abdominal fat accumulation. the present study utilized p-, t-, and s-line quail selected for more than 75 generations for high 4-wk body weight and also lines divergently selected (16 or more generations) for high (h-sd, h-cd) and low (l-sd, l-cd) 4-wk body weight. adult males had fr ...19902263537
pattern formation in the epithelium of the oviduct of japanese quail.cellular patterns of the oviduct epithelium from the japanese quail were examined during maturation. the epithelium of a juvenile bird showed a jigsaw puzzle pattern consisting of a single, undifferentiated cell type. at the start of maturation, cells were rearranged into a pattern in which the length of boundaries between goblet type gland (g-) cells and ciliated (c-) cells (g-c boundaries) were maximized. at this stage, the surface area of g-cells was much smaller, but g-cells are more than 2 ...20082268543
effect of snake venom of agkistrodon halys on atherosclerosis and blood characteristics in japanese quail.extracts of snake venom have been widely used for the treatment of vascular thrombotic diseases, yet the therapeutic mechanism is not clear. the effect of snake venom fractions on atherosclerosis in japanese quail was studied. the venom of agkistrodon halys was fractionated by deae-cellulose chromatography and the pooled protein fractions that resulted were injected intravenously into the quail with aortic atherosclerosis induced by dietary cholesterol. after 7 weeks of injections on every other ...19902282093
effects of the nonsteroidal inhibitor r76713 on testosterone-induced sexual behavior in the japanese quail (coturnix coturnix japonica).a new triazole derivative, r76713 (6-[4-chlorophenyl)(1h-1,2,4-triazol-1-yl)methyl]-1-methyl-1h- benzotriazole), was recently shown to inhibit aromatase selectively without affecting other steroid-metabolizing enzymes and without interacting with estrogen, progestin, or androgen receptors. this compound was tested for its capacity to intefere with the induction of copulatory behavior by testosterone (t) in castrated japanese quail (coturnix coturnix japonica). in a first experiment, r76713 inhib ...19902286366
temperature modulation of photoperiodically induced lh secretion and its termination in japanese quail (coturnix coturnix japonica).long days induced lh release in photosensitive japanese quail and ambient temperature did not affect this process. temperature also did not affect the levels of circulating lh concentrations at the steady state of lh release on long days. on the other hand, low ambient temperature was required to reduce circulating lh to a nonbreeding level together with the change of the photoperiod from long to short days; the changes of the photoperiod without low ambient temperature induced a decrease of cir ...19902289686
circulating estradiol and the activation of male and female copulatory behavior in japanese quail (coturnix japonica).previous experiments using systemic and preoptic area (poa) hormone treatments have shown that aromatization of testosterone (t) to estrogen (e) is essential for activation of male-typical copulatory behavior in castrated male japanese quail (coturnix japonica). two experiments were conducted to determine whether circulating estrogen levels characteristic of normal intact males are high enough to activate male-typical or female-typical copulatory behavior. in experiment 1, blood samples were dra ...19902307345
the cycle of the seminiferous epithelium in the japanese quail (coturnix coturnix japonica) and estimation of its duration.a regular, well defined spermatogenic cycle was found in the japanese quail by examining thin sections of isolated lengths of seminiferous tubules embedded in epoxy resin to resolve the structure of developing spermatids. the stages of the cycle initially were identified in studies using a preparatory method for fixation which separated adjacent cellular associations. the cycle was divided into 10 stages with relative frequencies (%) of stages i to x respectively of: 11.9, 14.8, 24.1, 10.3, 8.2, ...19902325016
comparative angiotoxic responses of avian and rodent species in vivo: implications in atherogenesis.as the intrinsic susceptibility to atherosclerosis differs among several taxonomic groups, the present studies were conducted to compare the angiotoxic responses of atherosclerosis-susceptible (quail) and -resistant (rat) animals to allylamine, a selective cardiovascular toxin. japanese quail (125-150 g) and sprague-dawley rats (175-200 g) were gavaged daily for 1, 7, or 20 d with allylamine hcl (0.7, 7, and 70 mg/kg) or tap water. at the ultrastructural level, subchronic exposure of quail and r ...19902325152
the induction by testosterone of aromatase activity in the preoptic area and activation of copulatory behavior.a series of 4 experiments was designed to study the relationships between the activity of the aromatase (aa) in the preoptic area (poa) and the activation by testosterone (t) of copulatory behavior in gonadectomized male and female japanese quail. the induction of aa by t in the poa is dose- and time-dependent. levels of aa seen in sexually mature males are restored in castrated birds by a treatment with 20 to 40 mm silastic t capsules which produce physiological levels of steroid in the plasma. ...19902326347
calcium and calmodulin levels in late embryonic and early hatched japanese quail brain.characterizing the last phases of embryonic avian brain development are increased brain activity and increased absorption of shell calcium. the calcium-binding protein, calmodulin, regulates many activities of calcium. in neural tissue, calmodulin modulates neural transmission, and is required for the phosphorylation of synaptosomal proteins. therefore, the objective was to compare levels of brain calcium and calmodulin in the japanese quail. brain extracts from embryonic days 11, 15, hatch, and ...20082327289
accumulation and distribution of ions of group-iiia elements in the tissues and eggs of the japanese quail.solutions of salts of the five group-iiia elements were given, intravenously, to mature japanese quail. by 18 h, the accumulation maxima in the major tissues were: leg bones, 20% for ga+3 (67ga label) in estradiol-treated males; liver, 51% for al+3 (26al label) in control males; kidneys, 32% for in+3 (114min label) in estradiol-treated males; and growing oocytes plus ova, 37% for in. accumulations of tl+1 (202tl label) were 6 times those for ga or in in the brain and muscles, and .1 times in pla ...19902330333
experimental infection of mice with toxocara canis larvae obtained from japanese quails.the migration and distribution of toxocara canis larvae in the tissues of japanese quails, infected orally with 5 x 10(3) infective eggs, were studied, as well as the re-infectivity of these larvae in mice, inoculated with 50 larvae obtained from the liver of these quails. post-infection, the highest concentrations of larvae were found to be present in the liver of quails while only a few migrated to other tissues like lungs, heart, muscle and brain. the migration and distribution of the larvae ...19902332284
anti-atherosclerotic activity of colestipol hydrochloride in sea quail.young, male, sea (susceptible to experimental atherosclerosis) japanese quail (coturnix coturnix japonica) were fed an atherogenic diet consisting of yellow corn meal and soybean meal supplemented with 2% cholesterol and 1% cholic acid. a control group of ten animals was fed the atherogenic diet for eight weeks, and another group was fed the same diet containing 2% colestipol hydrochloride for the same length of time. at the end of the treatment period serum and arterial total cholesterols were ...19902337384
nonspecific imprintability of quail to colors: response to artificial selection.eleven generations of bidirectionally selecting quail (c. coturnix japonica) for nonspecific imprintability to blue or red stimuli resulted in unexpectedly slow yet reliable differentiation of "hi" and "lo" imprintability lines. correlated changes were observed in the variances of unconditional color choices in subjects belonging to these genetic lines. implications of the data for understanding the interrelated expression of gene effects and environment effects in the development of behavior ar ...19902346472
infection of japanese quail with toxocara canis larvae and establishment of patent infection in pups.migration and distribution of toxocara canis larvae in the tissues of japanese quail, infected orally with 5 x 10(3) infective eggs, and the establishment of patent infection from the quails to the definitive host, were studied. larval yield at necropsy from various tissues and organs of quail varied from 4.72 to 7.54% of the infective eggs inoculated within the period 1-60 days post-infection (pi). the total number of larvae recovered on different days showed a gradual increase. the percent ino ...19902353428
protein requirement and amino acid imbalance in quail.1. growing japanese quail were fed on purified diets based either on dried egg albumen or casein supplemented with methionine. 2. at low protein concentration body mass, n-intake and n-growth requirement of chicks fed on diets containing casein were impaired compared to those fed on diets with egg albumen: in contrast at higher protein concentrations performance was better among those fed on diets with casein. 3. increased protein requirement was observed among birds fed on diets containing case ...19902354370
effect of diet and substrate on the in vitro measurement of cholesterol and fatty-acid synthesis in hepatic tissue of japanese quail.a comparison of acetate, glucose, mevalonate, or water as radioactive substrates for the hepatic synthesis of cholesterol, fatty acids, and glyceride-glycerol was made in japanese quail fed diets containing either beef fat or tuna oil. the quail fed a diet containing beef fat were fatter and had a significantly higher (p less than .01) concentration of serum cholesterol (5.6 mm per l) than that measured in the serum of quail given tuna oil (4.1 mm per l). both in vitro cholesterol and fatty-acid ...19902356181
sexual dimorphism in the avian brain is not limited to the song system of songbirds: a morphometric analysis of the brain of the quail (coturnix japonica).nissl-stained sections of adult male and female quail brains were examined for possible sex differences in the sizes of nuclei implicated in reproductive function or known to be dimorphic in songbirds, including nucleus magnocellularis preopticus, two additional preoptic nuclei, n. ventromedialis hypothalami, n. taeniae, n. hypoglossus and n. commissurae pallii. there was a significant sex difference in the volume of one of the preoptic nuclei (which was 38% larger in males), known to be a criti ...19902357544
immunocytochemical localization of aromatase in the brain.an immunocytochemical peroxidase-antiperoxidase procedure using a purified polyclonal antibody raised against human placental aromatase was used to localize aromatase-containing cells in the japanese quail brain. immunoreactive cells were found only in the preoptic area and hypothalamus, with a high density of positive cells being present in the sexually dimorphic medial preoptic nucleus, in the ventromedial nucleus of the hypothalamus and in the infundibulum. the positive material was localized ...19902357545
the effects of different iodide availabilities on thyroid function during development in japanese quail.japanese quail (coturnix japonica) were used to study the effects of different egg iodide (i) availabilities on thyroid function during development. low (less than 50 micrograms 1/kg feed in the maternal diet) and high (1200 micrograms 1/kg feed) i availability were compared to control levels (800 micrograms 1/kg feed), a standard supplementation for game bird feed. we measured thyroid gland content of i, triiodothyronine (t3) and thyroxine (t4), plasma concentrations of t3 and t4, hepatic 5' mo ...19902369822
the effects of hypergravitation, hypodynamy and their combination on the quantitative ultrastructure of the heart muscle of japanese quail. 19902371321
the effect of the angular velocity on the muscle composition in japanese quail. 19902371359
biopotency of methionine sources and their effects on sexual maturity of growing japanese quail.1. japanese quail were fed on practical diets supplemented with graded amounts of dl-methionine (dl-m), dl-methionine-sodium (dl-m-na), dl-methionine hydroxy-analogue calcium (dl-mha-ca) and dl-methionine hydroxy-analogue free acid (dl-mha-fa) on an equimolar basis. 2. by the slope ratio assay using quail growth as the criterion, it was deduced that the biological activity of dl-m-na, dl-mha-ca and dl-mha-fa was approximately 0.96, 0.72 and 0.84 that of dl-m on an equimolar basis. 3. on a weight ...19902372708
induction of hepatic cytochrome p-450 mediated alkoxyresorufin o-dealkylase activities in different species by prototype p-450 inducers.the induction of cytochrome p-450-mediated alkoxyresorufin o-dealkylase activities by various xenobiotics was examined in liver from a variety of animal species in order to gain insights into the substrate specificities of the induced p-450s. we found that forms of cytochrome p-450 capable of mediating the o-dealkylation of the short-chain phenoxazone ethers methoxy-, ethoxy- and propoxyresorufin were highly induced by 3-methylcholanthrene-type inducers and by aroclor-1254 in all species tested, ...19902379261
a new chondrodystrophy mutation in japanese quail (coturnix japonica).a second form of hereditary chondrodystrophy (ch-2) has been discovered in a selected line of japanese quail, coturnix japonica. this form of chondrodystrophy is autosomal and recessive, characterized by an overall shortening and bending of the long bones of the wings and legs, slight dwarfing of the trunk, bulging of the eyes, flattening of the head, and a parrot beak. the shortened long bones vary in regard to the amount of bending from nearly straight to bends of up to 90 degrees in the midsh ...20032380548
isolation of campylobacter jejuni from the eggs and organs in experimentally infected laying japanese quails (coturnix coturnix japonica). 19902385046
gene organization of the peking duck mitochondrial genome.the gene organization of the peking duck mitochondrial (mt)dna has been deduced through heterologous hybridization using different cloned fragments of the chicken or japanese quail mitochondrial genome as probes. as in the chicken, and other gallinaceous birds, the peking duck mtdna displays a novel gene order which differs from that of other vertebrates by the unusual localization of the trna(glu) and nd6 genes next to the displacement (d) loop region of the molecule. the position of these gene ...19902390786
comparison of hypocholesterolemic activities of the bile acid sequestrants cholestyramine and colestipol hydrochloride in cholesterol fed sea quail.the pharmacopolymer bile acid sequestrants cholestyramine and colestipol hydrochloride were mixed with a diet supplemented with 0.5% cholesterol at levels of 0.25%, 0.5%, and 1.0% for cholestyramine and 0.5% and 1.0% for colestipol and fed to young, male, sea (susceptible to experimental atherosclerosis) japanese quail (coturnix coturnix japonica) for a period of seven days. after treatment blood was obtained by venipuncture from non-fasted animals and analyzed for serum total cholesterol concen ...19902396902
presence of retrovirus reverse transcriptase-related gene sequences in avian cells lacking endogenous avian leukosis viruses.using a molecularly cloned viral dna probe representing the entire avian sarcoma virus (asv) reverse transcriptase (pol) gene, we have detected related sequences in dna preparations from two avain species, ev- chickens and japanese quail, previously demonstrated to lack all endogenous avain leukosis viruses. nucleotide sequence homology was detected only when hybridization conditions, which allowed the formation of stable duplexes with as much as 30% base mismatch, were used. no sequence homolog ...19852410912
hormonal correlates of gonadal regression and spontaneous recovery in japanese quail exposed to short day-lengths.adult male japanese quail were transferred from long to short days. plasma testosterone and dihydrotestosterone quickly decreased and this endocrine response was followed by a regression of the cloacal gland, an androgen-target organ. after about a month, a spontaneous recovery of gonadal activity was observed in some but not all birds. it was not associated with obvious shifts in the circadian system. the physiological bases of this spontaneous recovery are discussed as well as the detailed rel ...19852412507
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