Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a case of rhinocerebral zygomycosis caused by rhizopus arrhizus. | 2006 | 16796654 | |
| plasmids for expression of heterologous proteins in rhizopus oryzae. | rhizopus oryzae has long been used for enzyme production (e.g., glucoamylase and lipase), organic acid synthesis, and various fermented food applications. in this work, we describe a set of plasmid-based expression vectors that can be used for the production of heterologous proteins in r. oryzae. three plasmid vectors have been created using either the glucoamylase a (amya), pyruvate decarboxylase (pdca), or phosphoglycerate kinase (pgk1) promoters to drive expression of heterologous proteins. a ... | 2006 | 16804680 |
| empyema thoracis due to rhizopus oryzae in an allogenic bone marrow transplant recipient. | we describe a case of empyema thoracis caused by rhizopus oryzae diagnosed in an allogenic bone marrow transplant patient with acute lymphocytic leukemia. the isolate of r. oryzae was recovered from three pleural effusion specimens, which were black in color. it was identified on the basis of characteristic colonial appearance and microscopic findings, as well as the partial sequencing of rrna genes. the patient died of uncontrolled r. oryzae empyema thoracis and concomitant nosocomial infection ... | 2006 | 16805096 |
| biochemical and molecular characterization of a lipase produced by rhizopus oryzae. | a novel strain of rhizopus oryzae wpg secretes a noninduced lipase (rolw) in the culture medium; purified rolw is a protein of 29 kda, the 45 n-terminal amino acid residues were sequenced, this sequence is very homologous to rhizopus delemar lipase (rdl), rhizopus niveus lipase (rnl) and r. oryzae lipase (rol29) sequences; the cloning and sequencing of the part of the gene encoding the mature rolw, shows two nucleotides differences with rdl, rnl and rol29 sequences corresponding to the change of ... | 2006 | 16842350 |
| relationship between metabolism and biomass of medically important zygomycetes. | little is known about the relationships between metabolic activity and fungal biomass or time of incubation for medically important fungal pathogens. understanding these relationships may be especially relevant for rapidly growing organisms, such as zygomycetes. a range of inocula of five clinical isolates of zygomycetes (one each of rhizopus oryzae,rhizopus microsporus, cunninghamella bertholletiae, mucor circinelloides and absidia corymbifera) were incubated for 6, 8, 12, 24 and 48 h, after wh ... | 2006 | 16882609 |
| primary renal zygomycosis due to rhizopus oryzae. | primary renal zygomycosis is a rare fungal infection, and only 45 cases have been reported in the literature. the major etiologic agents of the disease were mucor spp., rhizomucor spp., rhizopus spp. and absidia spp. here we report a case of primary renal zygomycosis due to rhizopus oryzae infection. the patient had systemic lupus erythematosus and was treated with corticosteroids. he had frequent micturition, urodynia and passed amorphous and membranous-looking masses through the urethra severa ... | 2006 | 16882613 |
| construction of a pichia pastoris cell-surface display system using flo1p anchor system. | a pichia pastoris cell-surface display system was constructed using a flo1p anchor system, which was developed in saccharomyces cerevisiae. the lipase from rhizopus oryzae with a pro sequence (prorol) was used as the model protein and was genetically fused to the anchor consisting of amino acids 1-1099 of flo1p (fs anchor). the resulting fusion protein fsprorol was expressed under the control of the alcohol oxidase 1 promoter (paox1). the fluorescence microscopy of immunolabeled p. pastoris cell ... | 2006 | 16889374 |
| enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of (r,s)-1-benzyloxy-3-chloro-2-propyl monosuccinate. | rhizopus oryzae lipase (rol) was displayed on the cell surface of saccharomyces cerevisiae via the flo1 n-terminal region (1100 amino acids), which corresponds to a flocculation functional domain. the activity of lipase-displaying yeast whole-cell biocatalysts was enhanced 7.3-fold by incubation of the yeast cells at 20 degrees c in distilled water for 8 days after 8 day cultivation. the amount of lipase molecules present in cell wall and intracellular fractions was found to be increased 4.5- an ... | 2006 | 16889376 |
| adsorption behavior of rhodamine b on rhizopus oryzae biomass. | the removal of a carcinogenic dye rhodamine b (c. i. 45170) from wastewater by biomass of different moulds and yeasts is described. among all of the fungal species tested, the biomass of rhizopus oryzae mtcc 262 is found to be the most effective. dye adsorption reaches maximum with the biomass harvested from the early stationary phase of growth. the optimum temperature and ph for adsorption are observed to be 40 degrees c and 7.0, respectively. the adsorption rate is very fast initially and atta ... | 2006 | 16893225 |
| optimization of l-(+)-lactic acid production using pelletized filamentous rhizopus oryzae nrrl 395. | lactic acid is used as a food additive for flavor and preservation and a precursor in the development of poly-lactic acid, a product used to make biodegradable plastics and textiles. rhizopus oryzae nrrl 395 is known to be a strain that produces optically pure l-(+)-lactic acid. the morphology of rhizopus cultures is complex, forming filamentous, clumps, and pellet mycelia. different morphology growth has significant effects on lactic acid production. in bioreactors, the filamentous or clump myc ... | 2006 | 16915692 |
| deferiprone iron chelation as a novel therapy for experimental mucormycosis. | patients treated with the iron chelator deferoxamine are known to be more susceptible to mucormycosis. however, while deferoxamine is an iron chelator from the perspective of the human host, deferoxamine actually serves as a siderophore, delivering free iron to rhizopus oryzae, the major cause of mucormycosis. other iron chelators, including deferiprone, which do not deliver iron to r. oryzae have been described. we therefore sought to determine whether iron-chelation therapy with deferiprone wo ... | 2006 | 16928702 |
| functionality of selected strains of moulds and yeasts from vietnamese rice wine starters. | the role of starch-degrading mycelial fungi, and the alcohol production and ethanol tolerance of the yeasts isolated from selected vietnamese traditional rice wine starters were examined, and optimum conditions for these essential steps in rice wine fermentation were determined. of pure isolates from vietnamese rice wine starters, mould strains identified as amylomyces rouxii, amylomyces aff. rouxii, rhizopus oligosporus and rhizopus oryzae, were superior in starch degradation, glucose productio ... | 2006 | 16943022 |
| exploring amino acids responsible for the temperature profile of glycoside hydrolase family 45 endoglucanase egl3 from humicola grisea. | egl3 and rce1 are glycoside hydrolase family 45 endoglucanases isolated from humicola grisea and rhizopus oryzae respectively. the amino acid sequences of the two endoglucanases are homologous; on the other hand, the optimum temperature of egl3 is higher than that of rce1. in this study, four chimeric endoglucanases, named er1, er2, er3 and er4, in which one of four sequential amino acid regions of the egl3 catalytic domain (cad) was replaced by the corresponding rce1 amino acids, were construct ... | 2006 | 16960377 |
| a rhizopus oryzae strain isolated from resected bone and soft tissue specimens from a sinonasal and palatal mucormycosis case. report of a case and in vitro experiments of yeastlike cell development. | we report a histologically and mycologically proven sinonasal mucormycosis case causing palatal necrosis in a nondiabetic patient with renal failure. mycological examination of giemsa stained imprinted tissue preparations revealed abundant yeast-like cells besides the typical mucoraceous hyphae. the fungus was isolated from surgical specimens and identified as rhizopus oryzae by phenotypic and genotypic tests. laboratory studies were performed to investigate the association of the yeast-like cel ... | 2006 | 16966168 |
| molecular basis for enhanced activity of posaconazole against absidia corymbifera and rhizopus oryzae. | posaconazole and itraconazole were more potent inhibitors of ergosterol synthesis, in both intact cells and cell extracts from absidia corymbifera and rhizopus oryzae, than voriconazole and fluconazole. similarly, expression of cyp51 from r. oryzae in saccharomyces cerevisiae significantly increased resistance to fluconazole and voriconazole but not to posaconazole and itraconazole. | 2006 | 16966400 |
| biosorption of anionic textile dyes by nonviable biomass of fungi and yeast. | the nonviable biomass of aspergillus niger, aspergillus japonica, rhizopus nigricans, rhizopus arrhizus, and saccharomyces cerevisiae were screened for biosorption of textile dyes. the selected anionic reactive dyes were c.i. reactive black 8, c.i. reactive brown 9, c.i. reactive green 19, c.i. reactive blue 38, and c.i. reactive blue 3. experiments were conducted at initial dye concentration of 50, 100, 150 and 200mg/l. the effect of initial dye concentration, dose of biosorbent loading, temper ... | 2007 | 16997547 |
| cloning, disruption and protein secretory phenotype of the gas1 homologue of pichia pastoris. | the aim of the study was the identification, cloning and disruption of the gas1 homologue of pichia pastoris. gas1p is a glycoprotein anchored to the outer layer of the plasma membrane through a glycosylphosphatidylinositol (gpi) anchor. gas1p is a beta-1,3-glucanosyltransglycosylase (ec 2.4.1.-). this cross-linking enzyme highly affects the structure and permeability of the yeast cell wall. the gene coding for the gas1 homologue of p. pastoris was cloned by pcr, and its functionality was proven ... | 2006 | 17020547 |
| outbreak of cutaneous rhizopus arrhizus infection associated with karaya ostomy bags. | we investigated an outbreak involving 2 patients hospitalized at hospital a with cutaneous rhizopus arrhizus (oryzae) infections of surgically created stomas. | 2006 | 17029127 |
| amino acid regions of family 45 endoglucanases involved in cotton defibrillation and in resistance to anionic surfactants and oxidizing agents. | in the detergent industry, fungal endoglucanases are used to release microfibrils from the surfaces of dyed cellulosic fabrics to enhance color brightness. family 45 endoglucanase (glycoside hydrolase family 45, gh45) egl3 from humicola grisea is more resistant to anionic surfactants and oxidizing agents than family 45 endoglucanase rce1 from rhizopus oryzae, while in the present study, a catalytic domain of rce1 had higher defibrillation activity on dyed cotton fabrics than did that of egl3. to ... | 2006 | 17031034 |
| [screening of a low alcohol dehydrogenase activity mutant of rhizopus oryzae and the regulation of zn2+ and mg2+]. | ethanol is the main by-product in the fermentation broth of rhizopus oryzae as3.3461 for the production of high-optical purity l-lactic acid. alcohol dehydrogenase (adh) is the branch pathway enzyme that catalyzes the transformation of ethanol from pyruvate in rhizopus oryzae, which decreases the conversion rate of glucose to l-lactic acid. thus, screening the mutants with lower adh activity may increase lactate production dramatically. in present study, rhizopus oryzae as3.3461 was mutated with ... | 2006 | 17037060 |
| an early evolutionary origin for the minor spliceosome. | the minor spliceosome is a ribonucleoprotein complex that catalyses the removal of an atypical class of spliceosomal introns (u12-type) from eukaryotic messenger rnas. it was first identified and characterized in animals, where it was found to contain several unique rna constituents that share structural similarity with and seem to be functionally analogous to the small nuclear rnas (snrnas) contained in the major spliceosome. subsequently, minor spliceosomal components and u12-type introns have ... | 2006 | 17051219 |
| posaconazole prophylaxis in experimental systemic zygomycosis. | three isolates of zygomycetes belonging to two different genera (rhizopus oryzae and absidia corymbifera) were used to produce a systemic infection in neutropenic mice. on days -2 and -1 and at 2 h prior to infection, the mice received either posaconazole (pos) at doses ranging from 20 to 80 mg/kg of body weight/day or amphotericin b (amb) at 1 mg/kg/day. antifungal drug efficacy was assessed by determination of the prolongation of survival, determination of the percentage of infected organs (br ... | 2007 | 17060525 |
| continuous fixed bed biosorption of reactive dyes by dried rhizopus arrhizus: determination of column capacity. | a continuous fixed bed study was carried out by using dried rhizopus arrhizus as a biosorbent for the removal of three reactive dyes; gemacion (procion) red h-e7b (gr), a monoclorotriazine mono-azo type reactive dye; gemazol turquise blue-g (gtb), a vinyl sulfone mono-azo type reactive dye and gemactive (reactive) black hfgr (gb), a vinyl sulfone di-azo type reactive dye from aqueous solution. the effect of operating parameters such as flow rate and inlet dye concentration on the sorption charac ... | 2007 | 17070992 |
| application of response surface methodology for the biosorption of copper using rhizopus arrhizus. | response surface methodology was used to study the cumulative effect of the various parameters namely, initial copper ion concentration, ph, temperature, biomass loading and to optimize the process conditions for the maximum removal of copper. for obtaining the mutual interaction between the variables and optimizing these variables, a 2(4) full factorial central composite design using response surface methodology was employed. the analysis of variance (anova) of the quadratic model demonstrates ... | 2007 | 17084526 |
| combining regio- and enantioselectivity of lipases for the preparation of (r)-4-chloro-2-butanol. | preparation of 98% ee (r)-4-chloro-2-butanol was carried out by the enzymatic hydrolysis of chlorohydrin esters, using fungal resting cells and commercial enzymes. hydrolyzes were carried out using lipases from candida antarctica (novozym 435), c. rugosa, rhizomucor miehei (lipozyme im), burkolia cepacia, and resting cells of rhizopus oryzae and aspergillus flavus. the influence of the enzyme, the solvent, the temperature, and the alkyl chain length on the selectivity of hydrolyzes of isomeric m ... | 2007 | 17089342 |
| solution structure of family 21 carbohydrate-binding module from rhizopus oryzae glucoamylase. | cbms (carbohydrate-binding modules) function independently to assist carbohydrate-active enzymes. family 21 cbms contain approx. 100 amino acid residues, and some members have starchbinding functions or glycogen-binding activities. we report here the first structure of a family 21 cbm from the sbd (starch-binding domain) of rhizopus oryzae glucoamylase (rocbm21) determined by nmr spectroscopy. this cbm has a beta-sandwich fold with an immunoglobulin-like structure. ligand-binding properties of r ... | 2007 | 17117925 |
| divergent evolutionary lines of fungal cytochrome c peroxidases belonging to the superfamily of bacterial, fungal and plant heme peroxidases. | novel open reading frames coding for cytochrome c peroxidase (ccp) belonging to the superfamily of bacterial, fungal, and plant heme peroxidases were analyzed in the available fungal genomes. multiple sequence alignment of 71 selected peroxidase genes revealed the presence of three conserved regions essential for their function: one on the distal and two on the proximal side of the prosthetic heme group. conserved sequence motifs on the proximal heme side are peculiar for ccps and are responsibl ... | 2006 | 17126331 |
| a new approach of pellet formation of a filamentous fungus -rhizopus oryzae. | the effects of inoculum and medium composition (i.e. potato dextrose broth as carbon source, soybean peptone, calcium carbonate, and metal ions) on pellet formation of rhizopus oryzae atcc 20344 have been studied. metal ions were found to have a significant negative effect on pellet formation while soybean peptone had a positive effect. in addition, potato dextrose broth and calcium carbonate were beneficial to r. oryzae for growing small, smooth pellets during the culture. the study also demons ... | 2007 | 17175159 |
| zygomycosis of the penis due to rhizopus oryzae successfully treated with surgical debridement and a combination of high-dose liposomal and topical amphotericin b. | 2006 | 17179006 | |
| chromium(vi) biosorption by dried rhizopus arrhizus: effect of salt (nacl) concentration on equilibrium and kinetic parameters. | some industrial wastewaters contain high quantities of salts besides heavy metal ions. the presence of salt ions leads to high ionic strength, which may significantly affect the performance of the biosorption process so the effect of salts on the biosorption of heavy metal ions should be investigated. in this study the biosorption of chromium(vi) from saline solutions on dried rhizopus arrhizus was studied as a function of ph, initial chromium(vi) and salt (nacl) concentrations in a batch system ... | 2007 | 17188810 |
| lactate and ethanol productions by rhizopus oryzae atcc 9363 and activities of related pyruvate branch point enzymes. | in rhizopus oryzae cultivations, lactate production decreased and ethanol and biomass productions increased as inoculated spore concentration increased. spore concentrations of 1 x 10(3) and 1 x 10(4) spores ml(-1) induced tightly packed clumps, whereas 1 x 10(5) and 1 x 10(6) spores ml(-1) induced filamentous growth. | 2006 | 17189176 |
| in vitro antimycotic activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb. against opportunistic filamentous fungi. | xanthorrhizol was isolated from the rhizome of curcuma xanthorrhiza (zingiberaceae) and its in vitro activity against opportunistic filamentous fungi was evaluated using the nccls (m38-a) standard method. xanthorrhizol was found to be active against all the species tested, namely aspergillus flavus, aspergillus fumigatus, aspergillus niger, fusarium oxysporum, rhizopus oryzae and trichophyton mentagrophytes: the mics being 2.0, 2.0, 2.0, 4.0, 1.0 and 1.0 microg/ml, while the mfcs were 4.0, 4.0, ... | 2007 | 17236167 |
| biotechnological production of lactic acid integrated with fishmeal wastewater treatment by rhizopus oryzae. | fishmeal wastewater, a seafood processing waste, was utilized for production of lactic acid and fungal biomass by rhizopus oryzae as 3.254 with the addition of sugars. the 30 g/l exogenous glucose in fishmeal wastewater was superior to starch in view of productivities of lactic acid and fungal biomass, and cod reduction. fishmeal wastewater can be a replacement for peptone which was the most suitable nitrogen source for lactic acid production among the tested organic or inorganic nitrogen source ... | 2007 | 17242928 |
| preparation of a whole-cell biocatalyst of mutated candida antarctica lipase b (mcalb) by a yeast molecular display system and its practical properties. | to prepare a whole-cell biocatalyst of a stable lipase at a low price, mutated candida antarctica lipase b (mcalb) constructed on the basis of the primary sequences of calbs from c. antarctica cbs 6678 strain and from c. antarctica lf 058 strain was displayed on a yeast cell surface by alpha-agglutinin as the anchor protein for easy handling and stability of the enzyme. when mcalb was displayed on the yeast cell surface, it showed a preference for short chain fatty acids, an advantage for produc ... | 2007 | 17262207 |
| development of an oat-based biorefinery for the production of l(+)-lactic acid by rhizopus oryzae and various value-added coproducts. | a novel oat-based biorefinery producing l(+)-lactic acid and various value-added coproducts (e.g., beta-glucan, anti-irritant solution) is proposed. pearling is employed for sequential separation of bran-rich fractions for the extraction of value-added coproducts. lactic acid production is achieved via fungal fermentation of rhizopus oryzae on pearled oat flour. maximum lactic acid concentration (51.7 g/l) and starch conversion yield (0.68 g/g) were achieved when an oat flour concentration of 11 ... | 2007 | 17288441 |
| primary subcutaneous zygomycosis due to rhizopus oryzae in a 71-year-old man with normal immune status. | a case of primary subcutaneous zygomycosis due to rhizopus oryzae is described in a 71-year-old man with normal immune status. diagnosis was based on histological examination and culture of biopsy fragments from skin lesions. the patient recovered after itraconazole therapy (200 mg day(-1) for 60 days followed by 100 mg day(-1) for 45 days). | 2007 | 17302755 |
| carbon assimilation profiles as a tool for identification of zygomycetes. | identification of zygomycetes is difficult and time-consuming by standard microbiological procedures. carbon assimilation profiles are commonly used for yeast-and bacterial-species identification but rarely for filamentous-fungus identification. carbon assimilation profiles were evaluated using the commercialized kits id32c and api 50 ch, which contain 31 and 49 tests, respectively, to serve as simple tools for species identification of zygomycetes in clinical microbiology laboratories. fifty-se ... | 2007 | 17329449 |
| mucormycosis of the upper extremity in a patient with alcoholic encephalopathy. | cutaneous mucormycosis is a rare opportunistic infection caused by fungi of the class zygomycetes that can be rapidly fatal if unrecognized. the diagnosis of this infection is often made by infectious disease, dermatologic, or intensive care specialists. lesions that affect the upper limb may require a hand surgeon to diagnose the infection. the diagnosis may be difficult to make, because these infectious lesions can be confused with ischemic pathology. we report on a rare case of cutaneous muco ... | 2007 | 17336848 |
| construction of a novel synergistic system for production and recovery of secreted recombinant proteins by the cell surface engineering. | we determined whether the cocultivation of yeast cells displaying a zz-domain and secreting an fc fusion protein can be a novel tool for the recovery of secreted recombinant proteins. the zz-domain from staphylococcus aureus protein a was displayed on the cell surface of saccharomyces cerevisiae under the control of the gal1 promoter. strain s. cerevisiae by4742 cells displaying the zz-domain on their surface were used for cocultivation with cells that produce a target protein fused to the fc fr ... | 2007 | 17345082 |
| sorbitol co-feeding reduces metabolic burden caused by the overexpression of a rhizopus oryzae lipase in pichia pastoris. | to improve the specific production rate of rhizopus oryzae lipase (rol) in pichia pastoris, a protein that triggers the unfolded protein response in p. pastoris, the effect of sorbitol/methanol mixed substrates was tested in batch and fed-batch cultures. remarkably, a different substrate consumption behaviour was observed depending on the host's phenotype (mut(+) or mut(s)) in batch cultures: when the methanol assimilation capacity is genetically reduced (mut(s) phenotype), both substrates were ... | 2007 | 17399833 |
| stable and continuous long-term enzymatic reaction using an enzyme-nanofiber composite. | this study shows the preparation and application of enzyme-nanofiber composites for long-term stable operation. the enzyme-nanofiber composite was prepared by coating an enzyme aggregate, the esterase from rhizopus oryzae, on the surface of the nanofibers. after immobilization on the nanofiber, the apparent k ( m ) for the immobilized esterase was 1.48-fold higher than that of the free esterase, with values of 0.98 and 1.35 mm for the free and immobilized enzymes, respectively. it was found that ... | 2007 | 17404727 |
| production of butyl acetate ester by lipase from novel strain of rhizopus oryzae. | a new lipase preparation from rhizopus oryzae was used to catalyze the esterification reaction between acetic acid and butanol to produce butyl acetate ester (pineapple flavor). this flavor compound can be used in food, cosmetic and pharmaceutical industries. only 3% of butyl acetate was obtained when free lipase was used in the synthesis containing only the substrates. in contrast, the conversion yield reached 25% when immobilized lipase was used under the same conditions. the synthesis of buty ... | 2007 | 17502279 |
| isolation and characterization of two genes that encode active glucoamylase without a starch binding domain from rhizopus oryzae. | glucoamylase obtained from rhizopus sp. is frequently preferred for certain applications of starch modification or saccharification. the predominant enzyme, which contains a starch-binding domain on the amino terminus, has been previously characterized from several species. additionally, the cdna encoding this protein was cloned and found to show 100% identity to a r. oryzae strain 99-880 that has recently been sequenced by the broad institute of harvard and massachusetts institute of technology ... | 2007 | 17503147 |
| rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in pichia pastoris. | together with the development of optical sensors, fluorometry is becoming an increasingly attractive tool for the monitoring of cultivation processes. in this context, the green fluorescence protein (gfp) has been proposed as a molecular reporter when fused to target proteins to study their subcellular localization or secretion behaviour. the present work evaluates the use of the gfp fusion partner for monitoring extracellular production of a rhizopus oryzae lipase (rol) in pichia pastoris by me ... | 2007 | 17511861 |
| production of a rhizopus oryzae lipase from pichia pastoris using alternative operational strategies. | different cultivation strategies have been compared for the production of rhizopus oryzae lipase (rol) from pichia pastoris. several drawbacks have been found using a methanol non-limited fed-batch. on the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. a temperature limited fed-batch has been proposed to solve both problems. however, in our case study a methanol non-limited fed-batch results in better productivities. finally, ... | 2007 | 17544535 |
| native and modified lactate dehydrogenase expression in a fumaric acid producing isolate rhizopus oryzae 99-880. | rhizopus oryzae is subdivided into two groups based on genetic and phenotypic differences. type-i isolates accumulate primarily lactic acid when grown in the presence of a fermentable carbon source and contain two lactate dehydrogenase genes, ldha and ldhb. type-ii isolates synthesize predominantly fumaric acid and only have an ldhb gene. in this study, we determined that ldhb transcript is only minimally expressed in the type-ii isolate r. oryzae 99-880. ldhb enzyme purified from gene clones is ... | 2007 | 17551728 |
| study of pellet formation of filamentous fungi rhizopus oryzae using a multiple logistic regression model. | fungal pellet formation is an important topic of fermentation research. it has been reported that many factors such as agitation, medium nutrients, ph, polymer additives, and inoculum size influence the formation of fungal pellets. however, a few studies on the effects of all of these factors on fungal pellet formation have been reported. this paper conducted a comprehensive investigation using a completely randomized design (crd) on a filamentous fungus, rhizopus oryzae nrrl 395, in order to di ... | 2008 | 17570715 |
| in vitro susceptibility to fungicides by invertebrate-pathogenic and saprobic fungi. | the effect of five fungicides, benomyl (1 mg/l), dodine (50 mg/l), manzate (100 mg/l), cupric sulphate (200 mg/l) and thiabendazole (4 mg/l) was tested under in vitro conditions on development of 15 isolates of fungi pathogenic for insects and other invertebrates (beauveria brongniartii, culicinomyces clavisporus, duddingtonia flagrans, hirsutella thompsonii, two metarhizium anisopliae, nomuraea rileyi, two isaria/paecilomyces spp., and sporothrix insectorum) and 13 isolates of contaminant fungi ... | 2007 | 17574540 |
| zygomycosis--a case report and overview of the disease in india. | a case of zygomycosis caused by rhizopus oryzae in a diabetic patient previously misdiagnosed as invasive pulmonary aspergillosis and an overview of the disease in india are presented. the case was diagnosed by direct microscopy, histopathologic examination and culture. following surgical resection of pulmonary cavity under cover of amphotericin b administration, the patient recovered completely. of 461 cases reported to-date, approximately 70% had been diagnosed at the postgraduate institute of ... | 2007 | 17576314 |
| [cloning and expression of a new glucoamylase gene]. | according to the reported gene sequence of rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total dna of the natural rhizopus arrhizu. specific primers were designed to delete introns by overlapping pcr and a new cdna sequence of rhizopus arrhizu glucoamylase was obtained. the accession number in gene bank is dq903853. this gene is successfully expressed in the picha pastoris, producing a new protein with a high activity of glucoamylase. | 2007 | 17577997 |
| construction of a rhizopus arrhizus glucoamylase gene suitable for expression in distinct host: introns spliced artificially by pcr. | glucoamylase is an industrially extremely important enzyme in the fermentative production of ethanol, used in the enzymatic conversion of starch into high glucose and fructose syrups. the aim of this study is to construct a rhizopus arrhizus glucoamylase gene (raga)-introns artificially spliced by pcr-suitable for expression in s. cerevisiae host and tried expressing in picha pastoris. in previous work, we failed in amplifying glucoamylase gene from r. arrhizus by rt-pcr, so several primers were ... | 2008 | 17578683 |
| role of the linker region in the expression of rhizopus oryzae glucoamylase. | rhizopus oryzae glucoamylase (roga) consists of three domains: an amino (n)-terminal raw starch-binding domain (sbd), a glycosylated linker domain, and a carboxy (c)-terminal catalytic domain. the 36-amino-acid linker region (residues 132-167) connects the two functional domains, but its structural and functional roles are unclear. | 2007 | 17593302 |
| early use of posaconazole in the successful treatment of rhino-orbital mucormycosis caused by rhizopus oryzae. | mucormycosis is a potentially fatal, rapidly destructive, opportunistic infection often seen in immunocompromsied individuals and patients with diabetes mellitus. we describe two patients with rhino-orbital mucormycosis caused by rhizopus oryzae who received posaconazole within 7 days of diagnosis and in whom cure was achieved in conjunction with extensive surgical resection of diseased tissue. posaconazole was well-tolerated. our experience indicates that this new extended-spectrum azole antifu ... | 2007 | 17624436 |
| transcriptional response of p. pastoris in fed-batch cultivations to rhizopus oryzae lipase production reveals upr induction. | the analysis of transcriptional levels of the genes involved in protein synthesis and secretion is a key factor to understand the host organism's responses to recombinant protein production, as well as their interaction with the cultivation conditions. novel techniques such as the sandwich hybridization allow monitoring quantitatively the dynamic changes of specific rnas. in this study, the transcriptional levels of some genes related to the unfolded protein response (upr) and central metabolism ... | 2007 | 17634115 |
| synthesis, cocrystallization, and enzymatic degradation of novel poly(butylene-co-propylene succinate) copolymers. | a series of poly(butylene-co-propylene succinate) (pbpsu) random copolyesters were synthesized and characterized using 1hnmr spectroscopy and viscometry. tensile properties decreased with increasing propylene succinate (psu) content. cocrystallization and multiple melting behaviors were investigated. the copolymers showed a eutectic behavior. the minimum melting point corresponded to 75 mol % psu. wide-angle x-ray diffraction (waxd) patterns showed that the copolymers with up to 60 mol % psu uni ... | 2007 | 17655353 |
| [cutaneous zygomycosis caused by rhizopus arrhizus in a surgical wound.]. | we report a case of cutaneous zygomycosis caused by rhizopus arrhizus in a surgical wound following colostomy. the patient was a diabetic and in addition presented others risk factors. zygomycosis was suspected when a necrotic wound surface appeared in the appropiate clinical setting. diagnosis was confirmed on a combined histological and microbiological study. extensive surgical resection, high doses of amphotericin b and treatment of the underlying conditions permitted the control of the infec ... | 1998 | 17655419 |
| antimicrobial activity of methanol extract of origanum majorana l. (sweet marjoram). | in-vitro microbicidal activity of the methanol extract of origanum majorana l. was tested against seven fungi (fusarium solani, candida albicans, aspergillus niger, a. parasiticus, rhizopus oryzae, rhizoctonia otyzae-sativae and altemaria brassicicola) and six bacteria (bacillus subtilis, b. megaterium, escherichia coil, proteus vulgaris, pseudomonas aeruginosa and staphylococcus aureus). the methanol extract of o. majorana can be used as an effective herbal protectant against different pathogen ... | 2007 | 17718003 |
| colletotrichum circinans as a semiquantitative test unit for the growth substance produced by rhizopus suinus. | 1938 | 17773760 | |
| the iron chelator deferasirox protects mice from mucormycosis through iron starvation. | mucormycosis causes mortality in at least 50% of cases despite current first-line therapies. clinical and animal data indicate that the presence of elevated available serum iron predisposes the host to mucormycosis. here we demonstrate that deferasirox, an iron chelator recently approved for use in humans by the us fda, is a highly effective treatment for mucormycosis. deferasirox effectively chelated iron from rhizopus oryzae and demonstrated cidal activity in vitro against 28 of 29 clinical is ... | 2007 | 17786247 |
| fatty acid ethyl esters of rhizopus arrhizus. | gas chromatographic and mass spectrometric analyses on selected lipid fractions revealed for the first time the presence of ethyl esters of long-chain fatty acids as biological products. ethyl esters of oleic, palmitic, and stearic acids were detected in relative concentrations of 21.2, 2.4, and 1.5 percent, respectively, of the total methyl and ethyl ester fraction. both saturated and unsaturated ethyl esters contain pronounced mass spectral fragments at a mass-to-charge ratio of 88. | 1971 | 17792945 |
| removal of copper ions by the filamentous fungus, rhizopus oryzae from aqueous solution. | removal of heavy metals present in wastewaters has been a major concern due to their non-biodegradability and toxicity. removal of copper ion using naoh treated rhizopus oryzae biomass was investigated in a batch reactor. the copper uptake exhibited substantial enhancement both in terms of kinetics of uptake as well as the loading capacity. the copper biosorption by viable and pretreated fungal biomass fit well to a lagergren's pseudo second order reaction in comparison to pseudo first order kin ... | 2008 | 17804218 |
| cleavage of beta-carotene as the first step in sexual hormone synthesis in zygomycetes is mediated by a trisporic acid regulated beta-carotene oxygenase. | carotene cleavage is the necessary initial step in the biosynthesis of trisporic acid, the sexual signal in zygomycete fungi. two genes encoding putative carotene oxygenases, designated tsp3 and tsp4, were identified in the genome of the zygomycete rhizopus oryzae. using heterologous primers, tsp3 was cloned and sequenced also from blakeslea trispora. tsp3 transcription correlates with sexual development in both species. northern hybridization of b. trispora mrna revealed strong induction of tsp ... | 2007 | 17822929 |
| comparative study of thermostability and ester synthesis ability of free and immobilized lipases on cross linked silica gel. | a novel support has been utilized for immobilization of lipase, which was prepared by amination of silica with ethanolamine followed by cross linking with glutaraldehyde. lipases from rhizopus oryzae 3562 and enterobacter aerogenes were immobilized on activated silica gel, where they retained 60 and 50% of respective original activity. the thermal stability of the immobilized lipases was significantly improved in comparison to the free forms while the ph stability remained unchanged. e. aerogene ... | 2008 | 17882456 |
| enhancement of the stability of a prolipase from rhizopus oryzae toward aldehydes by saturation mutagenesis. | a prolipase from rhizopus oryzae (prorol) was engineered in order to increase its stability toward lipid oxidation products such as aldehydes with the aim of improving its performance in oleochemical industries. out of 22 amino acid residues (15 lys and 7 his) prone to react with aldehydes, 6 lys and all his residues (except for the catalytic histidine) were chosen and subjected to saturation mutagenesis. in order to quickly and reliably identify stability mutants within the resulting libraries, ... | 2007 | 17890336 |
| fermentation as a bio-process to obtain functional soybean flours. | the effect of fermentation on the antioxidant compounds [vitamins c and e, total phenolic compounds (tpc), and reduced glutathione (gsh)], and antioxidant capacity [superoxide anion scavenging activity (sod-like activity), peroxyl radical-trapping capacity (prtc), inhibition of phosphatidylcholine (pc) peroxidation, and trolox equivalent antioxidant capacity (teac)] of soybean (glycine max cv. merit) was studied. fermentation was carried out in solid state in cracked seeds inoculated with asperg ... | 2007 | 17907774 |
| identification of a novel delta9-fatty acid desaturase gene and its promoter from oil-producing fungus rhizopus arrhizus. | reverse transcriptional polymerase chain reaction (rt-pcr), rapid amplification of the cdna ends (race) and thermal asymmetric interlaced (tail)-pcr were used successfully to clone the open reading frame (1,377 bp) of delta9-fatty acid desaturase gene (named as rad9) and its promoter region from oil-producing fungi rhizopus arrhizus. functional identification of the protein was done by sub-cloning rad9 into the expression vector pyes2.0 to generate a recombinant plasmid pyrad9, which was then su ... | 2009 | 17934871 |
| co-production of fumaric acid and chitin from a nitrogen-rich lignocellulosic material - dairy manure - using a pelletized filamentous fungus rhizopus oryzae atcc 20344. | fumaric acid is widely used as a food additive for flavor and preservation. rhizopus oryzae atcc 20344 is a fungus known for good fumaric acid production. it also has been reported that the fungal biomass has high chitin content. this study investigated the possibility of producing both fumaric acid and chitin via r. oryzae fermentation of dairy manure. co-production of valuable bio-based chemicals such as fumaric acid and chitin could make the utilization of manure more efficient and more profi ... | 2008 | 18006305 |
| enhancement of growth and chitosan production by rhizopus oryzae in whey medium by plant growth hormones. | the effect of some plant growth hormones, viz., gibberellic acid, indole-3-acetic acid, indole-3-butyric acid, and kinetin on chitosan production by rhizopus oryzae in deproteinized whey was studied. hormones, at different concentrations, increase the mycelial growth by 19-32%. however, increase in chitosan content of the mycelia was relatively small (1.7-14.3%) over the control. maximum enhancement was observed with gibberellic acid. fifty percent more chitosan could be obtained from 1l of whey ... | 2008 | 18023862 |
| zygomycetes hyphae trigger an early, robust proinflammatory response in human polymorphonuclear neutrophils through toll-like receptor 2 induction but display relative resistance to oxidative damage. | human polymorphonuclear neutrophils (hpmns) displayed attenuated hyphal damage associated with impaired o(2)(-) release following exposure to rhizopus oryzae versus that with aspergillus fumigatus. exposure of hpmns to r. oryzae hyphae resulted in upregulation in toll-like receptor 2 mrna and a robust proinflammatory gene expression with rapid (1-h) induction of nf-kappab pathway-related genes. | 2008 | 18025115 |
| the logarithmic transformation should be avoided for stabilising the variance of mould growth rate. | radial growth rate, micro (mm d(-1)) was evaluated by plotting the radius of the colony, r (mm) versus time (d) for alternaria alternata, aspergillus flavus, cladosporium cladosporioides, mucor racemosus, rhizopus oryzae and trichoderma harzianum at different t and a(w). for each of the 12 data sets, an analysis of variance of the raw growth rate data was performed. it was observed from the p-values that all square-root transformed values of micro were non-significant at the significance level a ... | 2008 | 18031858 |
| improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain. | to utilize glucoamylase-displaying yeast cells for enzymatic desizing of starched cotton cloth, we constructed yeast strains that codisplayed rhizopus oryzae glucoamylase and two kinds of trichoderma reesei cellulose-binding domains (cbd1, cbd of cellobiohydrolase i (cbhi); and cbd2, cbd of cellobiohydrolase ii (cbhii)). in this study, we aimed to obtain a high efficiency of enzymatic desizing of starched cotton cloth. yeast cells that codisplayed glucoamylase and cbd had higher activity on star ... | 2008 | 18040681 |
| optimization of lipase pretreatment prior to lipase immobilization to prevent loss of activity. | in our previous work, a method of pretreating lipase was developed to prevent loss of its activity during covalent immobilization. in this study, rhizopus oryzae lipase was pretreated before immobilization and then immobilized on a silica gel surface. the effects of the various materials and conditions used in the pretreatment stage on the activity of immobilized lipase were investigated. immobilized lipase pretreated with 0.1% of soybean oil had better activity than those pretreated with other ... | 2007 | 18051278 |
| immunoreactivity and amino acid content of fermented soybean products. | food allergy has become a public health problem that continues to challenge both the public and the food industry. the objective of this research was the detection and quantification of the major human allergenic soy proteins and to study the reduction in immunoreactivity and improvement of amino acid content after fermentation of soybean flour. fermentation was carried out in the solid state of cracked seeds inoculated with aspergillus oryzae, rhizopus oryzae, and bacillus subtilis and in the l ... | 2008 | 18072744 |
| a rare case of mucormycosis of median sternotomy wound caused by rhizopus arrhizus. | we describe a case of mucormycosis of median sternotomy wound caused by rhizopus arrhizus . the patient, a known diabetic and a case of coronary artery disease underwent coronary artery bypass surgery. in the postoperative period, patient developed infection of the median sternotomy wound, from which r. arrhizus was isolated on culture. patient succumbed in spite of being treated with surgical debridement and amphotericin b. to the best of our knowledge, this is the first reported case of mucorm ... | 2007 | 18087101 |
| biochemical changes in micro-fungi fermented cassava flour produced from low- and medium-cyanide variety of cassava tubers. | comparative studies were carried out on the ability of pure strain of rhizopus oryzae and saccharomyces cerevisae to alter the nutritional quality of cassava flour produced from low- and medium-cyanide variety of cassava tuber. low- and medium-cyanide variety of cassava tubers were collected from international institute of tropical agriculture (iita), ibadan, nigeria. these tubers were washed, peeled, grated and aseptically inoculated with pure strains of rhizopus oryzae and saccharomyce cerevis ... | 2007 | 18087867 |
| enhanced citrate production through gene insertion in aspergillus niger. | the effect of inserting genes involved in the reductive branch of the tricarboxylic acid (tca) cycle on citrate production by aspergillus niger was evaluated. several different genes were inserted individually and in combination, i.e. malate dehydrogenase (mdh2) from saccharomyces cerevisiae, two truncated, cytosolic targeted, fumarases (fum1s and fumrs) from s. cerevisiae and rhizopus oryzae, respectively, and the cytosolic soluble fumarate reductase (frds1) from s. cerevisiae. overexpression o ... | 2008 | 18162426 |
| high-affinity iron permease (ftr1) gene sequence-based molecular identification of clinically important zygomycetes. | the clinical importance of zygomycosis, an emerging and frequently fatal mycotic disease, has increased during recent years. this report describes an identification method based on pcr amplification and sequencing of the high-affinity iron permease 1 gene (ftr1). primers and amplification protocols were established and tested for the identification of rhizopus oryzae, rhizopus microsporus var. rhizopodiformis, r. microsporus var. oligosporus, rhizopus schipperae, rhizopus niveus and rhizopus sto ... | 2008 | 18190575 |
| xylose metabolism in the fungus rhizopus oryzae: effect of growth and respiration on l+-lactic acid production. | the fungus rhizopus oryzae converts both glucose and xylose under aerobic conditions into chirally pure l+-lactic acid with by-products such as xylitol, glycerol, ethanol, carbon dioxide and fungal biomass. in this paper, we demonstrate that the production of lactic acid by r. oryzae cbs 112.07 only occurs under growing conditions. deprivation of nutrients such as nitrogen, essential for fungal biomass formation, resulted in a cessation of lactic acid production. complete xylose utilisation requ ... | 2008 | 18247072 |
| interaction of amphotericin b lipid formulations and triazoles with human polymorphonuclear leucocytes for antifungal activity against zygomycetes. | the frequency of zygomycosis has increased considerably over recent years mainly in immunocompromised and diabetic patients. little is known about the effects of host innate immunity against different zygomycetes especially under the influence of antifungal agents. the antifungal activity of human polymorphonuclear leucocytes (pmn) in combination with liposomal amphotericin b (lamb), amphotericin b lipid complex (ablc), voriconazole (vrc) and posaconazole (psc) against rhizopus oryzae and rhizop ... | 2008 | 18254752 |
| rhizopus delemar is the proper name for rhizopus oryzae fumaric-malic acid producers. | the zygomycete rhizopus oryzae currently is identified by sporangiophore morphology and growth temperature, but heterogeneity of the species has been reported. we examined the suitability of organic acid production as an effective taxonomic character for reclassification of the species. strains were divided into two groups, la (lactic acid producer) and fma (fumaric-malic acid producers) according to organic acid production. these grouping were confirmed as phylogenetically distinct because anal ... | 2007 | 18268905 |
| comparison of biosorption properties of different kinds of fungi for the removal of gryfalan black rl metal-complex dye. | three kinds of filamentous fungi (rhizopus arrhizus, trametes versicolor, aspergillus niger) were tested for their ability to adsorb gryfalan black rl metal-complex dye as a function of ph, temperature and dye concentration. r. arrhizus and t. versicolor exhibited the maximum dye uptake at ph 2.0 and at 25 degrees c while a. niger performed the highest dye biosorption at ph 1.0 and at 35 degrees c. sorption capacity of each biosorbent increased with increasing initial dye concentration. among th ... | 2008 | 18325761 |
| automated and manual methods of dna extraction for aspergillus fumigatus and rhizopus oryzae analyzed by quantitative real-time pcr. | quantitative real-time pcr (qpcr) may improve the detection of fungal pathogens. extraction of dna from fungal pathogens is fundamental to optimization of qpcr; however, the loss of fungal dna during the extraction process is a major limitation to molecular diagnostic tools for pathogenic fungi. we therefore studied representative automated and manual extraction methods for aspergillus fumigatus and rhizopus oryzae. both were analyzed by qpcr for their ability to extract dna from propagules and ... | 2008 | 18353931 |
| [diversity of facultatively anaerobic microscopic mycelial fungi in soils]. | the numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of cfu per one gram of soil; a total of 30 species was found. this group is composed primarily of mitotic fungi of the ascomycete affinity belonging to the orders hypocreales (fusarium solani, f. oxysporum, fusarium sp., clonostachys grammicospora, c. rosea. acremonium sp., gliocladium penicilloides, trichoderma aureoviride, t. harzianum, t. polysporum, t. viride. t. koning ... | 2008 | 18365728 |
| producing biodiesel using whole-cell biocatalysts in separate hydrolysis and methanolysis reactions. | this research examined the effect of using separate hydrolysis and methanolysis reactions for biodiesel production using a whole-cell biocatalysts derived from rhizopus oryzae (atcc 10260). biodiesel yield from separate hydrolysis and methanolysis was compared to transesterification reactions where both hydrolysis and methanolysis reactions occur in the same reactor. all reactions were conducted at room temperature. the effect of substituting ethanol for methanol was also studied. separating the ... | 2008 | 18393066 |
| adsorption of rhodamine b on rhizopus oryzae: role of functional groups and cell wall components. | the role of different functional groups (i.e. amino, carboxyl, hydroxyl as well as phosphate) and cell wall components (such as chitin, chitosan, glucan and phosphomannan) of rhizopus oryzae on adsorption of rhodamine b is described. the functional groups were chemically modified to determine their contribution in the present adsorption process. fourier transformed infrared spectroscopic (ftir) study was used to characterize the modification of the functional groups due to chemical treatments. c ... | 2008 | 18407472 |
| enhanced l-(+)-lactic acid production by an adapted strain of rhizopus oryzae using corncob hydrolysate. | corncob is an economic feedstock and more than 20 million tons of corncobs are produced annually in china. abundant xylose can be potentially converted from the large amount of hemicellulosic materials in corncobs, which makes the crop residue an attractive alternative substrate for a value-added production of a variety of bioproducts. lactic acid can be used as a precursor for poly-lactic acid production. although current industrial lactic acid is produced by lactic acid bacteria using enriched ... | 2008 | 18415989 |
| bioethanol production from uncooked raw starch by immobilized surface-engineered yeast cells. | surface-engineered yeast saccharomyces cerevisiae codisplaying rhizopus oryzae glucoamylase and streptococcus bovis alpha-amylase on the cell surface was used for direct production of ethanol from uncooked raw starch. by using 50 g/l cells during batch fermentation, ethanol concentration could reach 53 g/l in 7 days. during repeated batch fermentation, the production of ethanol could be maintained for seven consecutive cycles. for cells immobilized in loofa sponge, the concentration of ethanol c ... | 2008 | 18425612 |
| efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases. | aspergillus oryzae glucoamylases encoded by glaa and glab, and rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast saccharomyces cerevisiae gri-117-uk and laboratory yeast s. cerevisiae mt8-1. among constructed transformants, gri-117-uk/pudgaa, displaying glaa glucoamylase, produced the most ethanol from liquefied starch, although mt8-1/pudgar, displaying r. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface. | 2008 | 18460787 |
| development of an immunochromatographic lateral-flow device for rapid serodiagnosis of invasive aspergillosis. | aspergillus fumigatus is a cosmopolitan saprotrophic fungus that is second only to candida species as a cause of invasive fungal infections in immunocompromised humans. current immunodiagnostic tests for invasive aspergillosis (ia) are based on the detection of circulating galactomannan (gm) in a patient's serum by using a rat monoclonal antibody (mab), eb-a2, that binds to tetra (1-->5)-beta-d-galactofuranoside, the immunodominant epitope in gm. the potential cross-reactivity of mab eb-a2 with ... | 2008 | 18463222 |
| enhancement of rooibos (aspalathus linearis) aqueous extract and antioxidant yield with fungal enzymes. | the leaves and stems of the rooibos plant ( aspalathus linearis) are used for the production of an herbal tea known for its health promoting properties, which have been linked to its flavonoid content but which is substantially reduced by the traditional processing method employed. selected food-grade fungi were screened for their potential to improve the yield of soluble matter extracted from rooibos plant material. fungal cocktails of hydrolyzing enzymes enhanced either the yield of soluble so ... | 2008 | 18476700 |
| studying pellet formation of a filamentous fungus rhizopus oryzae to enhance organic acid production. | using pelletized fungal biomass can effectively improve the fermentation performance for most of fugal strains. this article studied the effects of inoculum and medium compositions such as potato dextrose broth (pdb) as carbon source, soybean peptone, calcium carbonate, and metal ions on pellet formation of rhizopus oryzae. it has been found that metal ions had significantly negative effects on pellet formation whereas soybean peptone had positive effects. in addition pdb and calcium carbonate w ... | 2007 | 18478426 |
| role of n-terminal 28-amino-acid region of rhizopus oryzae lipase in directing proteins to secretory pathway of aspergillus oryzae. | to develop a new approach for improving heterologous protein production in aspergillus oryzae, we focused on the functional role of the n-terminal region of rhizopus oryzae lipase (rol). several n-terminal deletion variants of rol were expressed in a. oryzae. interestingly, a segment of 28 amino acids from the c-terminal region of the propeptide (n28) was found to be critical for secretion of rol into the culture medium. to further investigate the role of n28, the rol secretory process was visua ... | 2008 | 18496686 |
| rhino-cerebral zygomycosis resistant to antimycotic treatment: a case report. | we report a fatal case of a rhino-cerebral zygomycosis, caused by rhizopus arrhizus (oryzae). the patient was suffering from idiopathic thrombopenic purpura, diagnosed 1 year earlier. he was already treated with methylprednisolone 5 months prior to his admission to the hospital for a loss of vision and pain in the left eye as well as left orbital cellulitis. after an initial empirical treatment with broad spectrum antibiotics and voriconazole (infection of unknown origin), the patient was treate ... | 2009 | 18498302 |
| production of l(+)-lactic acid using acid-adapted precultures of rhizopus arrhizus in a stirred tank reactor. | cultivations of filamentous fungi in stirred tank reactors (strs) to produce metabolites are often limited by insufficient mixing and mass transfer because of the formation of mycelial clumps inside the reactors. this study developed an acid-adapted preculture approach to control the morphology of filamentous rhizopus arrhizus in a str, consequently to enhance the production yield and productivity of l(+)-lactic acid efficiently using waste potato starch as substrate. using the acid-adapted prec ... | 2008 | 18500587 |
| caspofungin-mediated beta-glucan unmasking and enhancement of human polymorphonuclear neutrophil activity against aspergillus and non-aspergillus hyphae. | we investigated whether caspofungin and other echinocandins have immune-enhancing properties that influence human polymorphonuclear neutrophil (pmn)-mediated mold hyphal damage. | 2008 | 18500936 |
| the mechanism of uranium biosorption by rhizopus arrhizus. | biosorption of elements is a little understood phenomenon exhibited by some types of even nonliving microbial biomass. a common fungus rhizopus arrhizus has been reported to take up uranium from aqueous solutions to the extent of 180 mg u(6+)/g. the mechanism of uranium sequestering by this type of biomass was studied by using experimental techniques such as electron microscopy, x-ray energy dispersion analysis, ir spectroscopy, and supporting evidence was obtained for a biosorption mechanism co ... | 1982 | 18546310 |
| the mechanism of thorium biosorption by rhizopus arrhizus. | inactive cells of rhizopus arrhizus have been documented to exhibit a high thorium biosorptive uptake (170 mg/g) from aqueous solutions. the mechanism of thorium sequestering by this biomass type was investigated following the same method as for the uranium biosorption mechanism. the thorium sequestering mechanism appeared somewhat different from that of uranium. experimental evidence is presented which indicates that, at optimum biosorption ph (4), thorium coordinates with the nitrogen of the c ... | 1982 | 18546382 |
| the role of chitin in uranium adsorption by r. arrhizus. | in order to further refine and support the uranium biosorption mechanism hypothesis proposed for rhizopus arrhizus, uranium competitive equilibrium uptake isotherms by chitin were determined at two different solution ph levels and in the presence of different concentrations of competing ions, namely, cu(2+), zn(2+), and fe(2+). the co-ion effect became more poronounced as the co-ion concentration in solution and ph increased. obtained equilibrium data are in agreement with uranium biosorption da ... | 1983 | 18551548 |
| ion exchange/complexation of the uranyl ion by rhizopus biosorbent. | nonliving biomass of nine rhizopus species effectively sequestered the uranyl ion from solution, taking up 150-250 mg u/g dry cells at 300 ppm u equilibrium concentration in solution, and 100-160 mg u/g dry cells with 100 ppm u in solution. the affinity of this biosorbent for the uranyl ion was found to be affected by timing of harvesting and medium composition. uptake of the uranyl ion by nonliving biomass of rhizopus oligosporus was due to ion exchange or complexation, since binding was revers ... | 1984 | 18551656 |
| improved rate of fumaric acid production by tweens and vegetable oils in rhizopus arrhizus. | 1985 | 18553780 |