Publications

centella asiatica extract protects against amyloid β1-40-induced neurotoxicity in neuronal cells by activating the antioxidative defence system.	centella asiatica ( léi gōng gēn) is a traditional medicinal herb with high antioxidant activity, which decreases amyloid-β (aβ) deposition in the brain. at the same time, aggregated aβ-induced oxidative stress is the trigger in the pathogenesis of alzheimer's disease (ad). here, we investigated the ability of c. asiatica ethanol extract (cae) to protect pc12 and imr32 cells from aβ1-40-induced production of reactive oxygen species (ros) and concomitant neurotoxicity. aggregated aβ1-40 treatment ...	2016	27774420
effect of a mixture of diosmin, coumarin glycosides, and triterpenes on bleeding, thrombosis, and pain after stapled anopexy: a prospective, randomized, placebo-controlled clinical trial.	we evaluated the efficacy of oral administration of a mixture of diosmin, coumarin glycosides, and centella asiatica (venoplant®) in preventing bleeding, pain, and thrombosis of internal and external hemorrhoids after stapled anopexy (sa).	2017	27815700
in vitro and in vivo antioxidant, anti-hyperlipidemic properties and chemical characterization of centella asiatica (l.) extract.	the study aimed to identify the phenolic compounds present in centella asiatica (l.) (c. asiatica) extract and evaluate the respective antioxidant potential as well as its cholesterol-lowering effects in the experimental animal model. herein, the antioxidant potential of extracts was assessed by its free radical scavenging activity such as 2, 2-diphenyl -1- picrylhydrazyl as well as reducing capability. the anti-hyperlipidemic effects of c. asiatica extract (cae) were evaluated in high cholester ...	2016	27840607
synergistic combinations of five single drugs from centella asiatica for neuronal differentiation.	to identify alternatives of nerve growth factor, which could promote nf68 protein expression and contribute toward neuronal differentiation, five compounds namely: asiatic acid, madecassic, madecassoside, quercetin, and isoquercetin, obtained from centella asiatica, were examined for their neuronal differentiation effects on pc12 cells. c. asiatica has been applied as an effective herbal medicine for the treatment of various diseases, including depression. according to a statistical design of ex ...	2017	27893605
pharmacokinetics of a standardized extract of centella asiatica eca 233 in rats.	eca 233, a standardized extract of centella asiatica, has been found to exhibit various positive neurological effects and to have a good safety profile. the present study aimed to explore the disposition kinetics of eca 233, containing madecassoside (53.1 %) and asiaticoside (32.3 %), in rats. the extract was intravenously or orally administered at doses from 50 to 200 mg/kg. plasma, tissues, urine, and feces were collected at time points from 0 to 48 h after dosing. the levels of madecassoside ...	2016	27992940
comparative pharmacokinetics between madecassoside and asiaticoside presented in a standardised extract of centella asiatica, eca 233 and their respective pure compound given separately in rats.	1. eca 233, the standardised extract of centella asiatica, contains not less than 80% triterpenoid glycosides, in a madecassoside:asiaticoside ratio of 1.5 (±0.5):1. 2. the pharmacokinetic comparison of madecassoside and asiaticoside was performed in rats following intravenous and oral administration of eca 233, or an equivalent dose of the individual compounds. blood, tissues, urine and faeces were collected after dosing to determine drug and metabolite levels using liquid chromatography-tandem ...	2017	28001462
asiatic acid attenuates pre-neoplastic lesions, oxidative stress, biotransforming enzymes and histopathological alterations in 1,2-dimethylhydrazine-induced experimental rat colon carcinogenesis.	asiatic acid (aa), a pentacyclic triterpenoid, derived from the tropical medicinal plant centella asiatica is known to exhibit numerous pharmacological properties. we hypothesized that aa will have chemopreventive potential against 1,2-dimethylhydrazine (dmh)-induced experimental colon carcinogenesis in male wistar rats. rats were arbitrarily divided into six groups. group i rats were processed as control. group ii rats received aa (8 mg/kg b.w., p.o.) and groups iii-vi rats received subcutaneou ...	2017	28004603
asiatic acid enhances nrf2 signaling to protect hepg2 cells from oxidative damage through akt and erk activation.	asiatic acid (aa), a natural triterpene isolated from the plant centella asiatica, have antioxidative potential, but the molecular mechanism of aa against oxidative stress remains unclear. our study was performed to investigate the antioxidative effect of aa against oxidative stress and the antioxidative mechanism in tert-butyl hydroperoxide (t-bhp) -stimulated the hepg2 cells. the results showed that aa suppressed t-bhp-induced cytotoxicity, apoptosis, and reactive oxygen species (ros) generati ...	2017	28110191
asiatic acid inhibits lung cancer cell growth in vitro and in vivo by destroying mitochondria.	asiatic acid (aa), a pentacyclic triterpene found in centella asiatica, displays significant anti-proliferative effects on cancer cells in vitro although the underlying mechanism of this effect remains unknown. this study investigated the efficacy and mechanism of action of aa against lung cancer both in vivo and in vitro. using the mtt assay, aa was found to induce apoptosis in a dose- and time-dependent manner, an effect enhanced by pretreatment with an autophagy inhibitor. it also elevated ex ...	2017	28119810
neurotrophic effect of asiatic acid, a triterpene of centella asiatica against chronic 1-methyl 4-phenyl 1, 2, 3, 6-tetrahydropyridine hydrochloride/probenecid mouse model of parkinson's disease: the role of mapk, pi3k-akt-gsk3β and mtor signalling pathways.	regulation of various signalling (ras-mapk, pi3k and akt) pathways by augmented activity of neurotrophic factors (ntfs) could prevent or halt the progress of dopaminergic loss in parkinson's disease (pd). various in vitro and in vivo experimental studies indicated anti-parkinsonic potential of asiatic acid (aa), a pentacyclic triterpene obtained from centella asiatica. so the present study is designed to determine the neurotrophic effect of aa against 1-methyl 4-phenyl 1, 2, 3, 6-tetrahydropyrid ...	2017	28181071
asiaticoside: attenuation of rotenone induced oxidative burden in a rat model of hemiparkinsonism by maintaining the phosphoinositide-mediated synaptic integrity.	asiaticoside (as), a triterpenoid saponin isolated from the indian medicinal herb centella asiatica is known to exert a neuroprotective effect by attenuating the neurobehavioral, neurochemical and pathological changes in animal models. however, its potential neuroprotection in rotenone-induced hemiparkinsonism which implicates phospholipid-mediated neurotransmission remains unclear. therefore, we have investigated the neuroprotective effects of as in rat model of rot-infused hemiparkinsonism wit ...	2017	28238857
partial-thickness burn wounds healing by topical treatment: a randomized controlled comparison between silver sulfadiazine and centiderm.	burns are common event and associated with a high incidence of death, disability, and high costs. centella asiatica (l.) is a medicinal herb, commonly growing in humid areas in several tropical countries that improve wound healing. on the basis of previous studies, we compared the efficacy of centiderm versus silver sulfadiazine (ssd) in partial thickness burning patients.	2017	28248871
ethnobotanical survey of herbal tea plants from the traditional markets in chaoshan, china.	herbal tea, which refers to "cooling tea", "cool beverage", or "liáng chá" in china, includes a range of drinks with heat-clearing and detoxification qualities. herbal tea plants are great contributive to the health and prosperity of chaoshan people. the aim of the study was to document herbal tea plant species used and commercialized as "liáng chá" in chaoshan area, to facilitate the use and development of herbal tea enterprises, and to promote the further development of national herbal tea.	2017	28249822
centella asiatica attenuates aβ-induced neurodegenerative spine loss and dendritic simplification.	the medicinal plant centella asiatica has long been used to improve memory and cognitive function. we have previously shown that a water extract from the plant (caw) is neuroprotective against the deleterious cognitive effects of amyloid-β (aβ) exposure in a mouse model of alzheimer's disease, and improves learning and memory in healthy aged mice as well. this study explores the physiological underpinnings of those effects by examining how caw, as well as chemical compounds found within the extr ...	2017	28279707
asiatic acid ameliorates pulmonary fibrosis induced by bleomycin (blm) via suppressing pro-fibrotic and inflammatory signaling pathways.	idiopathic pulmonary fibrosis is known as a life-threatening disease with high mortality and limited therapeutic strategies. in addition, the molecular mechanism by which pulmonary fibrosis developed is not fully understood. asiatic acid (aa) is a triterpenoid, isolated from centella asiatica, exhibiting efficient anti-inflammatory and anti-oxidative activities. in our study, we attempted to explore the effect of asiatic acid on bleomycin (blm)-induced pulmonary fibrosis in mice. the findings in ...	2017	28320097
effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella (centella asiatica (l.) urban) cells.	in this study, we report the expression level of casqs, cabas and cacys, the genes involved in phytosterol and triterpene metabolic pathway of centella (centella asiatica (l.) urban), in cells elicited with salicylic acid (50-200 µm). reverse transcription-polymerase chain reaction (rt-pcr) and northern blot analysis indicated casqs, cabas, and cacys genes expressed in both the wild-type and cultured cells (with and without elicitation). in elicited cells, expressions of casqs, cabas, and cacys ...	2016	28330156
endophytic fungal isolate mediated biosynthesis of silver nanoparticles and their free radical scavenging activity and anti microbial studies.	the present study reports that the biosynthesis of agnps using an endophytic fungus isolated from the ethnomedicinal plant centella asiatica. the endophytic fungus was identified as aspergillus versicolor ent7 based on 18s rrna gene sequencing (ncbi accession number kf493864). the agnps synthesized were characterized by uv-visible spectroscopy, fourier transform infra-red spectroscopy (ftir), transmission electron microscopy (tem), x-ray diffraction (xrd), particle size analyzer, and zeta potent ...	2016	28330204
anti-inflammatory effect of titrated extract of centella asiatica in phthalic anhydride-induced allergic dermatitis animal model.	centella asiatica has potent antioxidant and anti-inflammatory properties. however, its anti-dermatitic effect has not yet been reported. in this study, we investigated the anti-dermatitic effects of titrated extract of centella asiatica (teca) in a phthalic anhydride (pa)-induced atopic dermatitis (ad) animal model as well as in vitro model. an ad-like lesion was induced by the topical application of five percent pa to the dorsal skin or ear of hos:hr-1 mouse. after ad induction, 100 μl of 0.2% ...	2017	28358324
madecassic acid, the contributor to the anti-colitis effect of madecassoside, enhances the shift of th17 toward treg cells via the pparγ/ampk/acc1 pathway.	the imbalance between th17 and treg cells substantially contributes to the intestinal immune disturbance and subsequent tissue injury in ulcerative colitis. the triterpenoid-rich fraction of centella asiatica was able to ameliorate dextran sulfate sodium-induced colitis in mice. here we explored its active ingredient and underlying mechanism with a focus on restoring the th17/treg balance. the four main triterpenoids occurring in c. asiatica were shown to attenuate colitis in mice by oral admini ...	2017	28358365
neuroprotective properties of madecassoside from centella asiatica after hypoxic-ischemic injury.	madecassoside is one of increasingly used constituent of centella asiatica, a frequently prescribed crude drug in south eastern asia and china for wound healing. in the present experiment, it exposes the neuroprotective nature of madecassoside in gt1-7 cell lines, further, which the antioxidant activities are performed. the cellular toxicity was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) assay with increased cell viability with ic50 2.5µg/ml. the regulation ...	2016	28375122
pva-peg physically cross-linked hydrogel film as a wound dressing: experimental design and optimization.	the development of hydrogel films as wound healing dressings is of a great interest owing to their biological tissue-like nature. polyvinyl alcohol/polyethylene glycol (pva/peg) hydrogels loaded with asiaticoside, a standardized rich fraction of centella asiatica, were successfully developed using the freeze-thaw method. response surface methodology with box-behnken experimental design was employed to optimize the hydrogels. the hydrogels were characterized and optimized by gel fraction, swellin ...	2017	28378604