Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| paenibacillus castaneae sp. nov., isolated from the phyllosphere of castanea sativa miller. | a bacterial strain, designated ch-32(t), was isolated from the phyllosphere of castanea sativa in spain. phylogenetic analysis based on 16s rrna gene sequences placed the isolate in the genus paenibacillus within the same subgroup as paenibacillus xinjiangensis and paenibacillus glycanilyticus, with similarities of 96.3 and 96.8 %, respectively. dna-dna hybridization values for strain ch-32(t) with these two species were lower than 20 %. the novel isolate was a gram-variable, motile, sporulating ... | 2008 | 18984693 |
| paenibacillus taichungensis sp. nov., from soil in taiwan. | among a large collection of taiwanese soil isolates, a novel gram-variable, rod-shaped, motile, endospore-forming bacterial strain, strain v10537(t), was subjected to a polyphasic study including 16s rrna and gyrb gene sequence analysis, dna-dna hybridization experiments, cell wall peptidoglycan type, cellular fatty acid composition analysis and comparative phenotypic characterization. 16s rrna gene sequence analysis indicated that the organism belonged to the genus paenibacillus. strain v10537( ... | 2008 | 18984707 |
| case report: paenibacillus thiaminolyticus: a new cause of human infection, inducing bacteremia in a patient on hemodialysis. | paenibacilli are gram-positive, aerobic bacteria that are related to bacilli but differ in the dna encoding their 16s rrna. until recently, these organisms were not known to cause human disease. there are now several reports of human infection caused by a few members of this genus, most commonly by p. alvei. we report a human infection in a patient with a permacath for chronic hemodialysis who was found to have bacteremia caused by p. thiaminolyticus, which is an environmental bacterium that has ... | 2008 | 18988935 |
| hydrogen production and anaerobic decolorization of wastewater containing reactive blue 4 by a bacterial consortium of salmonella subterranea and paenibacillus polymyxa. | anaerobic biodegradability of wastewater (3,000 mg codcr/l) containing 300 mg/l reactive blue 4, with different co-substrates, glucose, butyrate and propionate by a bacterial consortium of salmonella subterranea and paenibacillus polymyxa, concomitantly with hydrogen production was investigated at 35 degrees c. the accumulative hydrogen production at 3,067 mg codcr/l was obtained after 7 days of incubation with glucose, sludge, the bacterial consortium. the volatile fatty acids, residual glucose ... | 2009 | 19002762 |
| [composition and immunochemical characteristics of exopolysaccharides from the rhizobacterium paenibacillus polymyxa 1465]. | exopolysaccharides (eps) synthesized by paenibacillus polymyxa 1465 in the course of batch cultivation were proven to contain neutral and acidic fractions. eps are heterogeneous polysaccharides, represented by a complex of macromolecules with molecular mass of 7 x 10(4) to 2 x 10(6) da. the acidic component was shown to be predominant in eps preparations isolated from bacteria cultivated on glucose, which corresponds to a higher viscosity of eps water solutions. the exoglycans were shown to cont ... | 2008 | 19004343 |
| evaluation of culture media for paenibacillus larvae applied to studies of antimicrobial activity. | this study was conducted to compare different liquid culture media for paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. p. larvae presented poor growth in usual broths such as mueller-hinton, commonly employed in antimicrobial activity assays. growth in liquid media was evaluated using paenibacillus larvae strains isolated from hives located in different geographical zones. the myt medium (mueller-hin ... | 2008 | 19024500 |
| multiple-layer substrate zymography for detection of several enzymes in a single sodium dodecyl sulfate gel. | we have developed a system to detect three hydrolytic enzymes (cellulase, lipase, and protease) using a single sodium dodecyl sulfate (sds) gel and an electrotransfer system. after electrophoresis, proteins in the gel were transferred to three sandwiched substrate gels containing glycerol tributyrate, azo-carboxymethyl cellulose (azo-cmc), and fibrin for detection of cellulase, lipase, and protease, respectively. we show that three cellulases (from a paenibacillus sp. and two bacillus sp. strain ... | 2009 | 19059191 |
| isolation of bacillus and paenibacillus bacterial strains that produce large molecules of cyclic isomaltooligosaccharides. | cyclic isomaltooligosaccharides (cis) usually consist of 7 to 12 glucose units, although only ci-10 has strong inclusion complex-forming ability. four bacillus strains and two paenibacillus strains were isolated as novel ci-producing bacteria. among these, five strains produced small amounts of ci-7 to ci-9, but mainly produced ci-10 to ci-12. larger cis, up to ci-17, were also identified. | 2008 | 19060390 |
| biocontrol potential of soybean bacterial endophytes against charcoal rot fungus, rhizoctonia bataticola. | a total of 137 bacterial isolates from surface sterilized root, stem, and nodule tissues of soybean were screened for their antifungal activity against major phytopathogens like rhizoctonia bataticola, macrophomina phaseolina, fusarium udam, and sclerotium rolfsii. nine bacterial endophytes suppressed the pathogens under in vitro plate assay. these were characterized biochemically and identified at the genus level based on their partial sequence analysis of 16s rdna. eight of the isolates belong ... | 2009 | 19067044 |
| co-production of two new peptide antibiotics by a bacterial isolate paenibacillus alvei np75. | two new peptide antibiotics were secreted by a gram-positive bacterial strain isolated from fermented tomato fruit. based on its 99% 16s rdna sequence similarity with paenibacillus alvei, the isolate was designated as p. alvei np75. among these two peptides, one is active against gram-positive pathogens while the other against gram-negative pathogens; thus these peptides were named as paenibacillin p and paenibacillin n, respectively. after the purification of those peptide antibiotics from the ... | 2009 | 19073145 |
| paenibacillus tylopili sp.nov., a chitinolytic bacterium isolated from the mycorhizosphere of tylopilus felleus. | two chitinolytic bacterial strains (designated mk2(t) and v7) were isolated from the mycorhizosphere of the fungus tylopilus felleus. the strains were facultatively anaerobic g(+) endospore formers. physiological analysis and 16s rrna gene pcr-rflp assays revealed nearly identical profiles for both strains, demonstrating their relationship at the species level. sequences specific for the genus paenibacillus were found within the 16s rrna gene sequence of the strain mk2(t). the 16s rrna gene sequ ... | 2008 | 19085079 |
| the membrane lipoprotein lppx of paenibacillus sp. strain w-61 serves as a molecular chaperone for xylanase of glycoside hydrolase family 11 during secretion across the cytoplasmic membrane. | paenibacillus sp. strain w-61, which can utilize xylan as the sole source of carbon and energy, produces extracellular xylanases 1 and 3 (xyn1 and xyn3) and cell surface xylanase 5. in this study we found that lppx, immediately downstream of xyn1, encodes a lipoprotein located on the outer layer of the cytoplasmic membrane and that the lppx lipoprotein is essential for the secretion of active xyn1 across the cytoplasmic membranes. in escherichia coli, wild-type lppx was destined for the inner la ... | 2009 | 19103919 |
| paenibacillus harenae sp. nov., isolated from desert sand in china. | a gram-positive, endospore-forming, rod-shaped bacterium, designated strain b519t, was isolated from a desert sand sample of gansu province, china. strain b519t was strictly aerobic and cells were motile by means of peritrichous flagella. the strain grew optimally at 32-35 degrees c and ph 6.5-7.0. chemotaxonomic data supported the affiliation of the new isolate to the genus paenibacillus, including menaquinone-7 (mk-7) as the major isoprenoid quinone, dna g+c content of 49.9 mol%, cell-wall typ ... | 2009 | 19126715 |
| paenibacillus contaminans sp. nov., isolated from a contaminated laboratory plate. | a bacterial strain, designated ckobp-6t, isolated from a contaminated laboratory plate, was characterized by using a polyphasic taxonomic approach. chemotaxonomic and morphological properties of the strain were consistent with its classification in the genus paenibacillus. cells were gram-variable, aerobic, sporulating, motile rods. 16s rrna gene sequence analysis demonstrated that this novel isolate was unique, showing 94.6 % sequence similarity to paenibacillus chitinolyticus nbrc 15660t and l ... | 2009 | 19126735 |
| paenibacillus siamensis sp. nov., paenibacillus septentrionalis sp. nov. and paenibacillus montaniterrae sp. nov., xylanase-producing bacteria from thai soils. | three strains of xylanase-producing bacteria, s5-3(t), x13-1(t) and mxc2-2(t), isolated from soils in thailand, were characterized taxonomically based on their phenotypic and chemotaxonomic characteristics and 16s rrna gene sequence comparisons. they were gram-positive, facultatively anaerobic, spore-forming, rod-shaped bacteria. they contained meso-diaminopimelic acid in the cell-wall peptidoglycan. the dna g+c contents were respectively 45.8, 47.3 and 48.8 mol%. the predominant isoprenoid quin ... | 2009 | 19126736 |
| deadly competition between sibling bacterial colonies. | bacteria can secrete a wide array of antibacterial compounds when competing with other bacteria for the same resources. some of these compounds, such as bacteriocins, can affect bacteria of similar or closely related strains. in some cases, these secretions have been found to kill sibling cells that belong to the same colony. here, we present experimental observations of competition between 2 sibling colonies of paenibacillus dendritiformis grown on a low-nutrient agar gel. we find that neighbor ... | 2009 | 19129489 |
| decomposition and mineralization of aquatic humic substances (ahs) in treating landfill leachate using the anammox process. | aquatic humic substance (ahs) in landfill leachate is resistant to biodegradation, especially in anaerobic habitats. however, we reported here for the first time that ahs was completely biodegraded with the anaerobic ammonium oxidation (anammox) process. as a result of aromatic-ring cleavage and mineralization, carboxylic and aliphatic organics were significantly produced, the contents of chromophores such as quinoid and ketone were remarkably decreased to decolorize the leachate, and produced c ... | 2009 | 19131090 |
| [the study of mycolytic properties of aerobic spore-forming bacteria producing extracellular chitinases]. | the mycolytic activity of 27 strains of antagonistic bacilli belonging to two taxonomic groups (18 strains of bacillus subtilis and 9 strains of paenibacillus ehimensis) capable of induced synthesis of chitinolytic enzymes was studied. most of the b. subtilis strains neither displayed visible mycolytic effects on the phytopathogenic fungus bipolaris sorokiniana in vitro, nor produced chitinases in the presence of an auto-claved mycelium. on the contrary, p. ehimensis strains grown under conditio ... | 2008 | 19137718 |
| isolation and characterization of bacterial strains paenibacillus sp. and bacillus sp. for kraft lignin decolorization from pulp paper mill waste. | eight aerobic bacterial strains were isolated from pulp paper mill waste and screened for tolerance of kraft lignin (kl) using the nutrient enrichment technique in mineral salt media (msm) agar plate (15 g/l) amended with different concentrations of kl (100, 200, 300, 400, 500, 600 ppm) along with 1% glucose and 0.5% peptone (w/v) as additional carbon and nitrogen sources. the strains itrc s6 and itrc s8 were found to have the most potential for tolerance of the highest concentration of kl. thes ... | 2008 | 19164883 |
| [the role of paenibacillus polymyxa lectin in the macrophages-mediated killing of bacteria]. | influence of lectin lii of paenibacillus polymyxa 1460, specific for galactosamine, glucuronic acid, fructose-1,6-diphosphate, and glucosamine, on mechanisms of oxygen-dependent and oxygen-independent killing of bacteria was reviewed. it was shown that the lectin modulates the activity of some microbicide factors in the cytoplasm of macrophages before phagocytosis. increased activity of myeloperoxidase and acid phosphatase, enhanced formation of active forms of oxygen in alveolar macrophages, in ... | 2008 | 19186553 |
| isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the homestake gold mine, lead, south dakota, usa. | the present study investigated the cultivable mesophilic (37 degrees c) and thermophilic (60 degrees c) cellulose-degrading bacterial diversity in a weathered soil-like sample collected from the deep subsurface (1.5 km depth) of the homestake gold mine in lead, south dakota, usa. chemical characterization of the sample by x-ray fluorescence spectroscopy revealed a high amount of toxic heavy metals such as cu, cr, pb, ni, and zn. molecular community structures were determined by phylogenetic anal ... | 2009 | 19189143 |
| mutations at subsite -3 in cyclodextrin glycosyltransferase from paenibacillus macerans enhancing alpha-cyclodextrin specificity. | a major disadvantage of cyclodextrin production is the limited cyclodextrin product specificity of cyclodextrin glycosyltransferase (cgtase). here, we described mutations of asp372 and tyr89 at subsite -3 in the cgtase from paenibacillus macerans strain jfb05-01. the results showed that asp372 and tyr89 played important roles in cyclodextrin product specificity of cgtase. the replacement of asp372 by lysine and tyr89 by aspartic acid, asparagine, lysine, and arginine resulted in a shift in speci ... | 2009 | 19190904 |
| isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities. | this study was undertaken to survey culturable heterotrophic bacteria associated with the marine ark shell anadara broughtoni inhabiting in the sea of japan, and to test isolates for their antimicrobial, hemolytic and surface activities with an emphasis on low-molecular-weight metabolites search. a total of 149 strains were isolated and identified phenotypically. a total of 27 strains were selected to be investigated phylogenetically by 165 rrna gene sequence analysis. the most bacteria were aff ... | 2008 | 19216104 |
| characterization and partial purification of entomocin 110, a newly identified bacteriocin from bacillus thuringiensis subsp. entomocidus hd110. | a new bacteriocin produced by bacillus thuringiensis subsp. entomocidus was identified. the antibacterial activity termed entomocin 110 was produced starting at mid-logarithmic growth phase, reaching its maximum at the early and during stationary phase. the bacteriocin obtained from culture supernatant was inhibitory to several gram-positive bacteria including listeria monocytogenes, paenibacillus larvae and other bacillus species. entomocin 110 was shown to be heat stable and resistant to ph va ... | 2008 | 19216106 |
| paenibacillus polymyxa antagonizes oomycete plant pathogens phytophthora palmivora and pythium aphanidermatum. | to find sustainable alternatives to the application of synthetic chemicals for oomycete pathogen suppression. | 2009 | 19226403 |
| towards large-scale fame-based bacterial species identification using machine learning techniques. | in the last decade, bacterial taxonomy witnessed a huge expansion. the swift pace of bacterial species (re-)definitions has a serious impact on the accuracy and completeness of first-line identification methods. consequently, back-end identification libraries need to be synchronized with the list of prokaryotic names with standing in nomenclature. in this study, we focus on bacterial fatty acid methyl ester (fame) profiling as a broadly used first-line identification method. from the bame@lmg da ... | 2009 | 19237256 |
| paenibacillus thailandensis sp. nov. and paenibacillus nanensis sp. nov., xylanase-producing bacteria isolated from soil. | two strains of xylanase-producing bacteria, s3-4a(t) and mx2-3(t), isolated from soils in thailand, were characterized on the basis of their phenotypic and chemotaxonomic characteristics, dna-dna relatedness and 16s rrna gene sequences. the novel strains were gram-positive, facultatively anaerobic, spore-forming, rod-shaped bacteria. they contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. the dna g+c contents of strains s3-4a(t) and mx2-3(t) were 52 ... | 2009 | 19244442 |
| effects of chelating reagents on colonial appearance of paenibacillus alvei isolated from canine oral cavity. | a bacterial strain isolated from the oral cavity of a healthy dog revealed an unusual colony formation in nebular appearance on agar plates. the isolated bacterial strain was gram-positive, spore-forming rod with peritrichous flagella, and grown under aerobic conditions, but unable to grow at 45 degrees c. the strain was tentatively classified as paenibacillus alvei according to the biochemical properties and the 16s rrna gene sequence. the isolate exhibits collective locomotion on solid agar pl ... | 2009 | 19262024 |
| bionomics of a phoretic association between paenibacillus sp. and the entomopathogenic nematode steinernema diaprepesi. | spores of an unidentified bacterium were discovered adhering to cuticles of third-stage infective juvenile (ij) steinernema diaprepesi endemic in a central florida citrus orchard. the spores were cup-shaped, 5 to 6 mm in length, and contained a central endospore. based on 16s rdna gene sequencing, the bacterium is closely related to the insect pathogens paenibacillus popilliae and p. lentimorbus. however, unlike the latter bacteria, the paenibacillus sp. is non-fastidious and grew readily on sev ... | 2005 | 19262839 |
| [diversity of bacteria isolated from the south china sea sediments]. | to isolate bacteria from nansha area of south china sea, | 2008 | 19271531 |
| high rate of n2 fixation by east siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum. | for evaluating n(2) fixation of diazotrophic bacteria, nitrogen-poor liquid media supplemented with at least 0.5% sugar and 0.2% agar are widely used for acetylene reduction assays. in such a soft gel medium, however, many n(2)-fixing soil bacteria generally show only trace acetylene reduction activity. here, we report that use of a n(2) fixation medium solidified with gellan gum instead of agar promoted growth of some gellan-preferring soil bacteria. in a soft gel medium solidified with 0.3% ge ... | 2009 | 19286791 |
| molecular detection of nifh gene-containing paenibacillus in the rhizosphere of sorghum (sorghum bicolor) sown in cerrado soil. | to develop a polymerase chain reaction (pcr)-based approach for the detection of nifh gene-containing paenibacillus in environmental samples. | 2009 | 19291207 |
| detection and quantification of paenibacillus polymyxa in the rhizosphere of wild barley (hordeum spontaneum) with real-time pcr. | to detect and quantify the plant drought tolerance enhancing bacterium paenibacillus polymyxa in a collection of 160 hordeum spontaneum rhizosphere samples at the 'evolution canyon' ('ec'), israel. | 2009 | 19291233 |
| concurrent selection for microbial suppression of fusarium graminearum, fusarium head blight and deoxynivalenol in wheat. | identify biological agents that can both control fusarium head blight (fhb) and reduce deoxynivalenol (don) production. | 2009 | 19298518 |
| construction of a gene knockout system for application in paenibacillus alvei ccm 2051t, exemplified by the s-layer glycan biosynthesis initiation enzyme wsfp. | the gram-positive bacterium paenibacillus alvei ccm 2051t is covered by an oblique surface layer (s-layer) composed of glycoprotein subunits. the s-layer o-glycan is a polymer of [-->3)-beta-d-galp-(1[alpha-d-glcp-(1-->6)]-->4)-beta-d-manpnac-(1-->] repeating units that is linked by an adaptor of -[groa-2-->opo2-->4-beta-d-manpnac-(1-->4)]-->3)-alpha-l-rhap-(1-->3)-alpha-l-rhap-(1-->3)-alpha-l-rhap-(1-->3)-beta-d-galp-(1--> to specific tyrosine residues of the s-layer protein. for elucidation of ... | 2009 | 19304819 |
| negative correlation between individual-insect-level virulence and colony-level virulence of paenibacillus larvae, the etiological agent of american foulbrood of honeybees. | paenibacillus larvae is the etiological agent of american foulbrood (afb) in honeybees. recently, different genotypes of p. larvae (eric i to eric iv) were defined, and it was shown that these genotypes differ inter alia in their virulence on the larval level. on the colony level, bees mitigate afb through the hygienic behavior of nurse bees. therefore, we investigated how the hygienic behavior shapes p. larvae virulence on the colony level. our results indicate that p. larvae virulence on the l ... | 2009 | 19304833 |
| identification of a polymyxin synthetase gene cluster of paenibacillus polymyxa and heterologous expression of the gene in bacillus subtilis. | polymyxin, a long-known peptide antibiotic, has recently been reintroduced in clinical practice because it is sometimes the only available antibiotic for the treatment of multidrug-resistant gram-negative pathogenic bacteria. lack of information on the biosynthetic genes of polymyxin, however, has limited the study of structure-function relationships and the development of improved polymyxins. during whole genome sequencing of paenibacillus polymyxa e681, a plant growth-promoting rhizobacterium, ... | 2009 | 19304848 |
| towards functional proteomics of minority component of honeybee royal jelly: the effect of post-translational modifications on the antimicrobial activity of apalbumin2. | this study illustrates multifunctionality of proteins of honeybee royal jelly (rj) and how their neofunctionalization result from various ptms of maternal proteins. major proteins of rj, designated as apalbumins belong to a protein family consisting of nine members with m(r) of 49-87 kda and they are accompanied by high number of minority homologs derived from maternal apalbumins. in spite of many data on diversity of apalbumins, the molecular study of their individual minority homologous is sti ... | 2009 | 19322786 |
| diversity and phylogeny of culturable spore-forming bacilli isolated from marine sediments. | members of the genus bacillus and related genera are ubiquitous in nature. however, bacillus species isolated from marine sediments have attracted less interest respect to their terrestrial relatives. here, we report the phylogenetic diversity of a collection of 96 bacilli, isolated from 17 distinct stations of 5 oceanographic campaigns. the diversity was analysed by phenotypic and molecular approaches based on the amplified rdna restriction analysis (ardra), amplification of the internal transc ... | 2009 | 19322832 |
| cloning, sequencing, and expression of the gene encoding a multidomain endo-beta-1,4-xylanase from paenibacillus curdlanolyticus b-6, and characterization of the recombinant enzyme. | the nucleotide sequence of the paenibacillus curdlanolyticus b-6 xyn10a gene, encoding a xylanase xyn10a, consists of 3,828 nucleotides encoding a protein of 1,276 amino acids with a predicted molecular mass of 142,726 da. sequence analysis indicated that xyn10a is a multidomain enzyme comprising nine domains in the following order: three family 22 carbohydrate-binding modules (cbms), a family 10 catalytic domain of glycosyl hydrolases (xylanase), a family 9 cbm, a glycine-rich region, and three ... | 2009 | 19349753 |
| inhibition of paenibacillus larvae and ascosphaera apis by bacillus subtilis isolated from honeybee gut and honey samples. | three bacillus strains isolated from honey samples and bee gut were pre-selected for their in vitro antimicrobial activity against paenibacillus larvae and ascosphaera apis, important honeybee pathogens. the analysis of their 16s rrna sequences revealed that c4, m1 and g2iii strains belong to the subtilis species. surfactin synthesis was verified by ir spectroscopy and hplc studies. surfactin inhibited p. larvae but it failed to affect a. apis. vegetative cells of p. larvae were affected as soon ... | 2009 | 19358885 |
| cultivation of anaerobic and facultatively anaerobic bacteria from spacecraft-associated clean rooms. | in the course of this biodiversity study, the cultivable microbial community of european spacecraft-associated clean rooms and the herschel space observatory located therein were analyzed during routine assembly operations. here, we focused on microorganisms capable of growing without oxygen. anaerobes play a significant role in planetary protection considerations since extraterrestrial environments like mars probably do not provide enough oxygen for fully aerobic microbial growth. a broad assor ... | 2009 | 19363082 |
| tellurite resistance and reduction by a paenibacillus sp. isolated from heavy metal-contaminated sediment. | a gram-positive bacterium (designated as strain tew) that is highly resistant to tellurite was isolated from sediment. the bacterium can grow in the presence of up to 2,000 micromol/l of potassium tellurite (k2teo3). reduction of k2teo3 to tellurium was indicated by the blackening of the growth medium. no lag in growth was observed when cells unexposed to tellurite were transferred to the growth medium containing k2teo3, indicating that resistance to tellurite was not inducible. up to 50 and 90% ... | 2009 | 19374474 |
| fast identification of thermostable beta-glucosidase mutants on cellobiose by a novel combinatorial selection/screening approach. | engineering costly cellulases on natural cellulosic substrates is of importance for emerging biomass-based biorefineries. directed enzyme evolution is becoming a popular tool, but identification of desired mutants from a large mutant library remains challenging sometimes. in this work, we demonstrated a novel combinatorial selection/screening strategy for finding thermostable beta-glucosidase on its natural substrate-cellobiose. first, selection was conducted through complementation of beta-gluc ... | 2009 | 19388085 |
| glucose exerts a negative effect over a peroxidase from trichosporon asahii, with carotenoid cleaving activity. | tobacco aroma compounds were generated via lutein cleavage by the combined action of a yeast and a bacterium identified as trichosporon asahii and paenibacillus amylolyticus, respectively. in this study, an inverse relationship between glucose concentration and the generation of three compounds, present in the tobacco aroma profile, was observed in mixed cultures. in order to identify the organism sensitive to the sugar effect, both were grown separately. the presence of glucose suppressed beta- ... | 2009 | 19390852 |
| aldouronate utilization in paenibacillus sp. strain jdr-2: physiological and enzymatic evidence for coupling of extracellular depolymerization and intracellular metabolism. | paenibacillus sp. strain jdr-2, an aggressively xylanolytic bacterium isolated from decaying sweet gum wood, secretes a multimodular glycohydrolase family gh10 endoxylanase (xyna1) anchored to the cell surface. the gene encoding xyna1 is part of a xylan utilization regulon that includes an aldouronate utilization gene cluster with genes encoding a gh67 alpha-glucuronidase (agua), a gh10 endoxylanase (xyna2), and a gh43 arabinofuranosidase/beta-xylosidase (xynb). here we show that this paenibacil ... | 2009 | 19395566 |
| paenibacillus pueri sp. nov., isolated from pu'er tea. | pu'er tea is a fermented drink made from the leaves of the tea plant, camellia sinensis. two novel bacteria, designated strains b09i-3(t) and b13i-1, were isolated during the process of fermentation of this tea. these isolates were gram-positive, endospore-forming, motile rods that grew at 25-42 degrees c and ph 5.5-10.4. the dna g+c content was 56.6-58.4 mol%, the predominant isoprenoid quinone was mk-7 and the predominant cellular fatty acid was anteiso-c(15 : 0) (49.0-50 % of the total). phyl ... | 2009 | 19406782 |
| biodegradation aspects of polycyclic aromatic hydrocarbons (pahs): a review. | pahs are aromatic hydrocarbons with two or more fused benzene rings with natural as well as anthropogenic sources. they are widely distributed environmental contaminants that have detrimental biological effects, toxicity, mutagenecity and carcinogenicity. due to their ubiquitous occurrence, recalcitrance, bioaccumulation potential and carcinogenic activity, the pahs have gathered significant environmental concern. although pah may undergo adsorption, volatilization, photolysis, and chemical degr ... | 2009 | 19442441 |
| isolation and characterization of a multienzyme complex (cellulosome) of the paenibacillus curdlanolyticus b-6 grown on avicel under aerobic conditions. | a multienzyme complex, cellulosome, of the facultatively anaerobic bacterium, paenibacillus curdlanolyticus b-6 was produced on microcrystalline cellulose (avicel) under aerobic conditions. during growth on avicel, the bacterial cells were found to be capable of adhesion to avicel by scanning electron microscopic (sem) analysis. the multienzyme complex of p. curdlanolyticus b-6 was isolated from the crude enzyme preparation by gel filtration chromatography on sephacryl s-300 and affinity purific ... | 2009 | 19447336 |
| a novel endoglucanase (cel9p) from a marine bacterium paenibacillus sp. bme-14. | by constructing a genomic library, an endoglucanase gene (cel9p) was cloned from paenibacillus sp. bme-14 which was isolated from the sea. it had an open-reading frame of 1,629 bp, encoding a peptide of 542-amino acid residue with a calculated molecular mass of 60 kda. the enzyme showed the highest amino acid identity of 52% with other known endoglucanases and had a c-terminal catalytic domain belonging to the glycosyl hydrolases family 9. the optimum ph and temperature for enzymatic activity wa ... | 2010 | 19448979 |
| plant growth-promoting rhizobacteria, paenibacillus polymyxa and paenibacillus lentimorbus suppress disease complex caused by root-knot nematode and fusarium wilt fungus. | to screen and evaluate the biocontrol potential of paenibacillus strains against disease complex caused by meloidogyne incognita and fusarium oxysporum f. sp. lycopersici interactions. | 2009 | 19457027 |
| isolation and characterization of a protective bacterial culture isolated from honey active against american foulbrood disease. | bacterial strains isolated from us domestic honey were screened for antibacterial activity against paenibacillus larvae ssp. larvae, the causative agent of american foulbrood (afb) in apiaries. a bacterial isolate (th13) showing a high level of antimicrobial activity against p. larvae ssp. larvae atcc 9545 was selected and identified as paenibacillus polymyxa by 16s rrna gene sequencing. the antimicrobial compound was purified by 80% saturated ammonium sulfate precipitation followed by cm-sephar ... | 2009 | 19459943 |
| a novel beta-glucan produced by paenibacillus polymyxa jb115 induces nitric oxide production in raw264.7 macrophages. | the effect of extracellular beta-(1-->3), (1-->6)-glucan, produced by paenibacillus polymyxa jb115, on nitric oxide (no) production in raw264.7 macrophages was investigated. beta-glucan induced the production of no by raw264.7 macrophages in a concentration- and time-dependent manner. moreover, beta-glucan stimulation increased the mrna expression of inos, cox-2 and il-6 in raw264.7 macrophages in a concentration-dependent manner. | 2009 | 19461214 |
| phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control. | to study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria. | 2009 | 19486410 |
| paenibacillus pectinilyticus sp. nov., isolated from the gut of diestrammena apicalis. | during a search for exo-enzyme-producing bacteria in the gut of an insect, diestrammena apicalis, a novel bacterium capable of degrading pectin was isolated. the isolate, designated strain rcb-08(t), comprised gram-positive, endospore-forming, motile rods capable of growth at 15-30 degrees c and ph 6.0-8.7. the dna g+c content of the isolate was 51.5 mol% and the predominant cellular fatty acid was anteiso-c(15 : 0) (74.1 %). phylogenetic analysis based on 16s rrna gene sequences showed that str ... | 2009 | 19502313 |
| demonstration of catalytic proton acceptor of chitosanase from paenibacillus fukuinensis by comprehensive analysis of mutant library. | chitosanase from paenibacillus fukuinensis d2 is an attractive enzyme, and it exhibits both chitosanase and beta-1, 4 glucanase activities. in our previous study, we generated p. fukuinensis chitosanase-displaying yeast cells using a yeast cell surface-displaying system. chitosanase-displaying yeast can be utilized as a chitosanase cluster without many time-consuming purification steps. in this study, using the system, we have investigated whether glu302, which is supposed as a putative proton a ... | 2009 | 19517107 |
| molecular cloning and crystal structural analysis of a novel beta-n-acetylhexosaminidase from paenibacillus sp. ts12 capable of degrading glycosphingolipids. | we report the molecular cloning and characterization of two novel beta-n-acetylhexosaminidases (beta-hex, ec 3.2.1.52) from paenibacillus sp. strain ts12. the two beta-hexs (hex1 and hex2) were 70% identical in primary structure, and the n-terminal region of both enzymes showed significant similarity with beta-hexs belonging to glycoside hydrolase family 20 (gh20). interestingly, however, the c-terminal region of hex1 and hex2 shared no sequence similarity with the gh20 beta-hexs or other known ... | 2009 | 19524595 |
| guidelines for interpretation of 16s rrna gene sequence-based results for identification of medically important aerobic gram-positive bacteria. | this study is believed to be the first to provide guidelines for facilitating interpretation of results based on full and 527 bp 16s rrna gene sequencing and microseq databases used for identifying medically important aerobic gram-positive bacteria. overall, full and 527 bp 16s rrna gene sequencing can identify 24 and 40 % of medically important gram-positive cocci (gpc), and 21 and 34 % of medically important gram-positive rods (gpr) confidently to the species level, whereas the full-microseq a ... | 2009 | 19528178 |
| paenibacillus tundrae sp. nov. and paenibacillus xylanexedens sp. nov., psychrotolerant, xylan-degrading bacteria from alaskan tundra. | eight psychrotolerant, xylan-degrading strains of bacteria that were catalase-positive, oxidase-negative and able to reduce nitrate to nitrite were isolated from soil beneath moist non-acidic and acidic tundra in northern alaska. the dna g+c contents for the strains ranged from 46.4-50.3 mol%. phylogenetic analysis based on 16s rrna gene sequences revealed that each strain belonged to the genus paenibacillus. the highest level of 16s rrna gene similarity was found between the eight strains and p ... | 2009 | 19542122 |
| calcium leads to further increase in glycine-enhanced extracellular secretion of recombinant alpha-cyclodextrin glycosyltransferase in escherichia coli. | overexpression of recombinant genes in escherichia coli and targeting recombinant proteins to the culture medium are highly desirable for the production of industrial enzymes. however, a major barrier is inadequate secretion of recombinant protein across the two membranes of e. coli cells. in the present study, we have attempted to circumvent this secretion problem of the recombinant alpha-cyclodextrin glycosyltransferase (alpha-cgtase) from paenibacillus macerans strain jfb05-01. it was found t ... | 2009 | 19548680 |
| species-specific structural and functional diversity of bacterial communities in lichen symbioses. | lichens are generally considered as mutualisms between fungi and green algae or cyanobacteria. these partnerships allow light-exposed and long-living joint structures. the unique organization of lichens provides still unexplored environments for microbial communities. to study lichen-associated bacterial communities, we analyze samples, by a polyphasic approach, from three lichen species (cladonia arbuscula, lecanora polytropa and umbilicaria cylindrica) from alpine environments. our results ind ... | 2009 | 19554038 |
| a pcr method of detecting american foulbrood (paenibacillus larvae) in winter beehive wax debris. | the objective of this work was to create a fast and sensitive method of detecting paenibacillus larvae from beehive debris based on pcr that does not require long-lasting cultivation steps. various methods of extracting spores from beehive debris were compared: the original method of extraction of spores into toluene, and alternative spore extraction methods into tween 80, into water, into isopropanol and into 95% ethanol. isolation of dna from various spore extractions was evaluated too. best r ... | 2009 | 19559547 |
| broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis. | broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples. | 2009 | 19570210 |
| a metabolic signature of the beneficial interaction of the endophyte paenibacillus sp. isolate and in vitro-grown poplar plants revealed by metabolomics. | metabolic profiling via gas chromatography coupled to mass spectrometry was used to investigate the influence of endophytic bacteria on shoots of in vitro-grown poplar plants free from culturable endophytic bacteria. the results demonstrate that the occurrence of an endophytic paenibacillus strain strongly affects the composition of the plant metabolites of in vitro-grown poplars. eleven metabolites were significantly changed between inoculated and non-inoculated poplar plants as determined by t ... | 2009 | 19589078 |
| purification and characterization of a multienzyme complex produced by paenibacillus curdlanolyticus b-6. | paenibacillus curdlanolyticus b-6 showed effective degradation activities for xylan and cellulose and produced an extracellular multienzyme complex (approximately 1,450 kda) containing several xylanases and cellulases. to characterize the multienzyme complex, we purified the complex from culture supernatants by four kind of chromatography. the purified multienzyme complex was composed of a 280-kda protein with xylanase activity, a 260-kda protein that was a truncated form on the c-terminal side ... | 2010 | 19597812 |
| paenibacillus macquariensis subsp. defensor subsp. nov., isolated from boreal soil. | two gram-variable, aerobic, motile, rod-shaped, endospore-forming bacterial strains, m4-2t and m4-1, were isolated from soil samples collected from oblast magadan, russian far east, as micro-organisms antagonistic to the psychrophilic phytopathogenic fungus typhula ishikariensis. strains m4-2t and m4-1 were identified as members of the genus paenibacillus by phenotypic and phylogenetic analyses based on 16s rrna gene sequences. the strains contained anteiso-c15:0 as the major fatty acid (63.0-64 ... | 2009 | 19605719 |
| isolation, incubation, and parallel functional testing and identification by fish of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement. | this paper illustrates a plug-based microfluidic approach combining the technique of the chemistrode and the principle of stochastic confinement, which can be used to i) starting from a mixture of cells, stochastically isolate single cells into plugs, ii) incubate the plugs to grow clones of the individual cells without competition among different clones, iii) split the plugs into arrays of identical daughter plugs, where each plug contained clones of the original cell, and iv) analyze each arra ... | 2009 | 19606291 |
| bacteria in the gut of japanese honeybee, apis cerana japonica, and their antagonistic effect against paenibacillus larvae, the causal agent of american foulbrood. | we assessed the complexity of bacterial communities occurring in the digestive tract of the japanese honeybee, apis cerana japonica, using histological and 16s rrna gene sequence analyzes. both gram-positive and -negative bacteria were observed, and the number of gut bacteria was higher in old larvae compared with young larvae. a total of 35 clones were obtained by a culture-dependent method, and 16s rrna gene sequence analysis revealed that the bacterial population in the gut of japanese honeyb ... | 2009 | 19616552 |
| paenibacillus dendritiformis bacterial colony growth depends on surfactant but not on bacterial motion. | most research on growing bacterial colonies on agar plates has concerned the effect of genetic or morphotype variation. some studies have indicated that there is a correlation between microscopic bacterial motion and macroscopic colonial expansion, especially for swarming strains, but no measurements have been obtained for a single strain to relate the microscopic scale to the macroscopic scale. we examined here a single strain (paenibacillus dendritiformis type t; tip splitting) to determine bo ... | 2009 | 19617369 |
| anaerobic fermentation of glycerol in paenibacillus macerans: metabolic pathways and environmental determinants. | paenibacillus macerans is one of the species with the broadest metabolic capabilities in the genus paenibacillus, able to ferment hexoses, deoxyhexoses, pentoses, cellulose, and hemicellulose. however, little is known about glycerol metabolism in this organism, and some studies have reported that glycerol is not fermented. despite these reports, we found that several p. macerans strains are capable of anaerobic fermentation of glycerol. one of these strains, p. macerans n234a, grew fermentativel ... | 2009 | 19617389 |
| two naphthalene degrading bacteria belonging to the genera paenibacillus and pseudomonas isolated from a highly polluted lagoon perform different sensitivities to the organic and heavy metal contaminants. | two bacterial strains were isolated in the presence of naphthalene as the sole carbon and energy source from sediments of the orbetello lagoon, italy, which is highly contaminated with both organic compounds and metals. 16s rrna gene sequence analysis of the two isolates assigned the strains to the genera paenibacillus and pseudomonas. the effect of different contaminants on the growth behaviors of the two strains was investigated. pseudomonas sp. ornap2 showed a higher tolerance to benzene, tol ... | 2009 | 19621207 |
| paenibacillus cellulositrophicus sp. nov., a cellulolytic bacterium from thai soil. | a cellulolytic bacterium, strain p2-1(t), isolated from soil in thailand, was characterized using a taxonomic approach based on phenotypic and chemotaxonomic characteristics and the 16s rrna gene sequence. the novel strain was gram-positive, facultatively anaerobic, spore-forming and rod-shaped. it contained meso-diaminopimelic as the diagnostic diamino acid in the cell-wall peptidoglycan. the dna g+c content was 52.7 mol%. the major isoprenoid quinone was mk-7. anteiso-c(15 : 0) and iso-c(16 : ... | 2009 | 19625433 |
| paenibacillus sonchi sp. nov., a nitrogen-fixing species isolated from the rhizosphere of sonchus oleraceus. | a nitrogen-fixing bacterium, designated strain x19-5(t), was isolated from rhizosphere soil of sonchus oleraceus. phylogenetic analysis based on a fragment of the nifh gene and the full-length 16s rrna gene sequence revealed that strain x19-5(t) was a member of the genus paenibacillus. strain x19-5(t) showed the highest 16s rrna gene sequence similarity (98.8 %) with paenibacillus graminis rsa19(t) and below 97 % similarity with other recognized members of the genus. the level of dna-dna related ... | 2009 | 19625436 |
| endophytic bacteria associated with growing shoot tips of banana (musa sp.) cv. grand naine and the affinity of endophytes to the host. | a cultivation-based assessment of endophytic bacteria present in deep-seated shoot tips of banana suckers was made with a view to generate information on the associated organisms, potential endophytic contaminants in tissue-cultured bananas and to assess if the endophytes shared a beneficial relationship with the host. plating the tissue homogenate from the central core of suckers showed colony growth on nutrient agar from just 75% and 42% of the 12 stocks during may and november, respectively ( ... | 2009 | 19633807 |
| [phylogenetic diversity of endophytic endospore-forming bacteria isolated from cinnamomum longepaniculatum n. chao ex h. w. li]. | in order to study the diversity of endophytic endospore-forming bacteria in cinnamomum longepaniculatum. | 2009 | 19637562 |
| [effects of endophytic paenibacillus polymyxa s-7 on photosynthesis, yield, and quality of sugar beet]. | this study showed that inoculation of endophytic paenibacillus polymyxa s-7 could significantly (p < 0.05) promote the photosynthesis of sugar beet. after the inoculation, the leaf net photosynthetic rate (pn), stomatal limitation (ls), stomatal conductance (gs), and transpiration rate (tr) increased by 16.11%, 23.82%, 41.91%, and 34.80%, respectively, while the stomatal intercellular co2 concentration (ci) decreased by 21.09%. inoculation of endophytic p. polymyxa s-7 could also increase sugar ... | 2009 | 19637598 |
| characterization of secreted proteases of paenibacillus larvae, potential virulence factors involved in honeybee larval infection. | paenibacillus larvae is the causative agent of american foulbrood (afb), the most severe bacterial disease that affects honeybee larvae. afb causes a significant decrease in the honeybee population affecting the beekeeping industry and agricultural production. after infection of larvae, p. larvae secretes proteases that could be involved in the pathogenicity. in the present article, we present the secretion of different proteases by p. larvae. inhibition assays confirmed the presence of metallop ... | 2009 | 19638278 |
| transfer of bacillus mucilaginosus and bacillus edaphicus to the genus paenibacillus as paenibacillus mucilaginosus comb. nov. and paenibacillus edaphicus comb. nov. | bacillus mucilaginosus and bacillus edaphicus were reclassified based on their 16s rrna and gyrb gene sequences, dna-dna hybridization, fatty acid methyl esters and other taxonomic characteristics. phylogenetic analysis based on 16s rrna and gyrb gene sequences indicated that strains of b. mucilaginosus and b. edaphicus were members of the genus paenibacillus, with over 90.4 % and 70.3 % sequence similarity, respectively. their dna g+c contents were 54.5-56.8 mol%. the dna-dna relatedness values ... | 2010 | 19643872 |
| survey of bacterial populations present in us-produced linerboard with high recycle content. | to survey paperboard products from 17 us mills for bacterial populations and for bacteria potentially harmful to human health. | 2010 | 19645765 |
| paenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of triticum aestivum. | a bacterial strain designated sbr5(t) was isolated from the rhizosphere of triticum aestivum. a phylogenetic analysis based on the 16s rrna gene sequence placed the isolate within the genus paenibacillus, being most closely related to paenibacillus graminis rsa19(t) (98.1 % similarity). the isolate was a gram-reaction-variable, motile, facultatively anaerobic bacterium, with spores in a terminal position in cells. starch was utilized and dihydroxyacetone and catalase were produced. strain sbr5(t ... | 2010 | 19648317 |
| fontibacillus aquaticus gen. nov., sp. nov., isolated from a warm spring. | a novel facultatively anaerobic strain, designated gptsa 19(t), was isolated from a warm spring and characterized using a polyphasic approach. the strain behaved as gram-negative in the gram staining procedure but showed a gram-positive reaction in the aminopeptidase test. the novel strain was a mesophilic rod with ellipsoidal endospores. on the basis of 16s rrna gene sequence analysis, the strain showed closest similarity (96.0 %) with paenibacillus motobuensis mc10(t). the gene sequence simila ... | 2010 | 19651726 |
| paenibacillus aestuarii sp. nov., isolated from an estuarine wetland. | a novel bacterial strain designated cj25(t) was isolated from the estuarine wetland of the han river in korea. identification of this strain was carried out on the basis of polyphasic taxonomy. the isolate was gram-staining-positive, rod-shaped, non-pigmented and motile. phylogenetic analysis based on the 16s rrna gene sequence revealed that the isolate was closely related to paenibacillus chondroitinus dsm 5051(t) with 96.1 % similarity. the predominant fatty acids were anteiso-c(15 : 0) (50.25 ... | 2010 | 19654345 |
| delayed supplementation of glycine enhances extracellular secretion of the recombinant alpha-cyclodextrin glycosyltransferase in escherichia coli. | the targeting of recombinant proteins for secretion to the culture medium of escherichia coli presents significant advantages over cytoplasmic or periplasmic expression. however, a major barrier is inadequate secretion across two cell membranes. in the present study, we attempted to circumvent this secretion problem of the recombinant alpha-cyclodextrin glycosyltransferase (alpha-cgtase) from paenibacillus macerans strain jfb05-01. it was found that glycine could promote extracellular secretion ... | 2010 | 19655137 |
| characterisation of microbial flocs formed from raw textile wastewater in aerobic biofilm reactor (abr). | microbial flocs formed from raw textile wastewater in a prototype aerobic biofilm reactor (abr) system were characterised and studied for their potential use in the treatment of textile wastewater. after 90-100 days of operation, microbial flocs of loose irregular structures were obtained from the reactor with good settling velocity of 33 m/h and sludge volume index (svi) of 48.2 ml/g. molecular analysis of the flocs using pcr-amplified 16s rdna sequence showed 98% homology to those of bacillus ... | 2009 | 19657163 |
| cohnella fontinalis sp. nov., a xylanolytic bacterium isolated from fresh water. | a novel xylan-degrading bacterium, yt-1101(t), was isolated from fresh water. the isolate was a gram-reaction-negative, aerobic, motile, endospore-forming and rod-shaped bacterium. phylogenetic analysis of the 16s rrna gene sequence indicated that strain yt-1101(t) belonged to the genus cohnella, sharing sequence similarities of less than 94 % with the type species. the genomic g+c content was 58.6 mol%. the predominant menaquinone was mk-7. the major fatty acids were anteiso-c(15 : 0), iso-c(16 ... | 2010 | 19667368 |
| paenibacillus hunanensis sp. nov., isolated from rice seeds. | four gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains fel05(t), fel11, fek19 and fek21, were isolated from seeds of hybrid rice (oryza sativa l. jinyou 611), and their taxonomic positions were determined using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences showed that the four strains were members of the genus paenibacillus. they showed 95.4 % sequence similarity or less with strains of othe ... | 2010 | 19667385 |
| paenibacillus pocheonensis sp. nov., a facultative anaerobe isolated from soil of a ginseng field. | a gram-positive, aerobic or facultatively anaerobic, rod-shaped, spore-forming bacterium, strain gsoil 1138(t), was isolated from soil of a ginseng field in pocheon province, south korea, and was characterized in order to determine its taxonomic position. on the basis of 16s rrna gene sequence analysis, strain gsoil 1138(t) was shown to belong to the family paenibacillaceae and was most closely related to the type strains of paenibacillus chondroitinus (98.2 % similarity) and paenibacillus algin ... | 2010 | 19667397 |
| characterisation of a detergent-stable alkaline protease from a novel thermophilic strain paenibacillus tezpurensis sp. nov. as-s24-ii. | an alkaline-protease-producing bacterial strain (as-s24-ii) isolated from a soil sample in assam is a gram-stain-positive, catalase-positive, endospore-forming rod and grows at temperatures ranging from 30 degrees c to 60 degrees c and salinity ranging from 0% to 7% (w/v) nacl. phenotypic characterisation, chemotaxonomic properties, presence of paenibacillus-specific signature sequences, and ribotyping data suggested that the strain as-s24-ii represents a novel species of the genus paenibacillus ... | 2010 | 19669756 |
| the prospects of cellulase-producing bacteria for the bioconversion of lignocellulosic biomass. | lignocellulosic biomass is a renewable and abundant resource with great potential for bioconversion to value-added bioproducts. however, the biorefining process remains economically unfeasible due to a lack of biocatalysts that can overcome costly hurdles such as cooling from high temperature, pumping of oxygen/stirring, and, neutralization from acidic or basic ph. the extreme environmental resistance of bacteria permits screening and isolation of novel cellulases to help overcome these challeng ... | 2009 | 19680472 |
| the innate immune and systemic response in honey bees to a bacterial pathogen, paenibacillus larvae. | there is a major paradox in our understanding of honey bee immunity: the high population density in a bee colony implies a high rate of disease transmission among individuals, yet bees are predicted to express only two-thirds as many immunity genes as solitary insects, e.g., mosquito or fruit fly. this suggests that the immune response in bees is subdued in favor of social immunity, yet some specific immune factors are up-regulated in response to infection. to explore the response to infection m ... | 2009 | 19695106 |
| biphenyl-metabolizing bacteria in the rhizosphere of horseradish and bulk soil contaminated by polychlorinated biphenyls as revealed by stable isotope probing. | dna-based stable isotope probing in combination with terminal restriction fragment length polymorphism was used in order to identify members of the microbial community that metabolize biphenyl in the rhizosphere of horseradish (armoracia rusticana) cultivated in soil contaminated with polychlorinated biphenyls (pcbs) compared to members of the microbial community in initial, uncultivated bulk soil. on the basis of early and recurrent detection of their 16s rrna genes in clone libraries construct ... | 2009 | 19700551 |
| xanthan and gellan degradation by bacteria of activated sludge. | owing to increasing amounts of xanthan and gellan in food, cosmetics and pharmaceuticals, as well as in some technical spheres, studies were carried out on the xanthan and gellan degrading bacteria present in activated sludge. the activated sludge used in the study was able to degrade both carbohydrates over 7 days, with levels of xanthan and gellan utilizing microbes estimated at 10(5) cells/g of dry sludge weight. isolating key degrading bacteria revealed the important role of genus paenibacil ... | 2009 | 19700835 |
| bacterial populations associated with mycelium of the arbuscular mycorrhizal fungus glomus intraradices. | abstract the influences of the arbuscular mycorrhizal fungus glomus intraradices on the culturable aerobic-heterotrophic bacterial communities in the rhizosphere and hyphosphere of cucumber plants (cucumis satvius) were investigated. mycorrhizal and non-mycorrhizal plants were grown in compartmentalised growth units, each with a root compartment and two lateral root-free compartments. samples representing rhizosphere, root-free soil, root-free sand and washed sand extract were collected 52 days ... | 2002 | 19709247 |
| first report of paenibacillus cineris from a patient with cystic fibrosis. | we describe the recovery of paenibacillus cineris from a brazilian cystic fibrosis (cf) patient. paenibacillus spp. are mainly environmental organisms. although the role that these species play in lung disease is not known, we highlight the importance of careful attention to unusual bacteria from respiratory secretions of cf patients. | 2010 | 19709843 |
| removal of cadmium from aqueous solution by polysaccharide produced from paenibacillus polymyxa. | this paper deals with the removal of cadmium from aqueous solutions by polysaccharide produced from paenibacillus polymyxa. the effects of contact time, initial metal ions concentration, mass of the polysaccharide and ph were studied. the freundlich and dubinin-radushkevich (d-r) models have been applied and the equilibrium adsorption was found to best fit the dubinin-radushkevich adsorption isotherm based on the coefficient of correlation, r(2). the maximum cd(2+) uptake was 520.09 mg g(-1). an ... | 2009 | 19713040 |
| mutations of lysine 47 in cyclodextrin glycosyltransferase from paenibacillus macerans enhance beta-cyclodextrin specificity. | the nature of amino acid residue 47 shows a clear discrimination between the different groups of cyclodextrin glycosyltransferase (cgtase). the effects of amino acid side chain at position 47 on cyclodextrin product specificity were investigated by replacing lys47 in the cgtase from paenibacillus macerans strain jfb05-01 with arginine, histidine, threonine, serine, or leucine. all of the mutations reduced alpha-cyclodextrin-forming activity, whereas significant increases in beta-cyclodextrin-for ... | 2009 | 19715296 |
| uncovering the protocatechuate 2,3-cleavage pathway genes. | paenibacillus sp. (formerly bacillus macerans) strain jj-1b is able to grow on 4-hydroxybenzoate (4hb) as a sole source of carbon and energy and is known to degrade 4hb via the protocatechuate (pca) 2,3-cleavage pathway. however, none of the genes involved in this pathway have been identified. in this study, we identified and characterized the jj-1b genes for the 4hb catabolic pathway via the pca 2,3-cleavage pathway, which consisted of prar and praabegfdchi. based on the enzyme activities of ce ... | 2009 | 19717587 |
| structure of a novel highly branched alpha-glucan enzymatically produced from maltodextrin. | the bacterial strain pp710, isolated from soil and identified as paenibacillus species, produced a low-digestibility alpha-glucan containing a large amylase-resistant portion. this alpha-glucan was obtained in high yields from maltodextrin (dextrose equivalent 3) by using the condensed culture supernatant of the strain as the enzyme preparation. the water-soluble dietary fiber content of the low-digestibility alpha-glucan was 80.2%, and showed resistance to a rat intestinal enzyme preparation. t ... | 2009 | 19740459 |
| pesticide tolerance of paenibacillus sp. d1 and its chitinase. | excessive use of pesticides in agriculture has led to several problems pertaining to loss of soil fertility and environmental degradation. biological control agents offer the best alternative to reduce use of toxic pesticides. paenibacillus sp. d1 isolated from the effluent treatment plant of a seafood processing industry exhibited broad spectrum tolerance towards a number of pesticides at concentrations higher than recommended for field applications. the isolate showed enhanced growth and chiti ... | 2009 | 19758745 |
| high temperature, short time pasteurization temperatures inversely affect bacterial numbers during refrigerated storage of pasteurized fluid milk. | the grade a pasteurized milk ordinance specifies minimum processing conditions of 72 degrees c for at least 15 s for high temperature, short time (htst) pasteurized milk products. currently, many us milk-processing plants exceed these minimum requirements for fluid milk products. to test the effect of pasteurization temperatures on bacterial numbers in htst pasteurized milk, 2% fat raw milk was heated to 60 degrees c, homogenized, and treated for 25 s at 1 of 4 different temperatures (72.9, 77.2 ... | 2009 | 19762797 |