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serological detection of avian reovirus in different poultry flocks of gazipur and mymensingh districts of bangladesh.avian reovirus (arv) is a constraint to poultry industry in bangladesh as a cause of several diseases in chickens, especially in broiler. however, the actual status of the viral infection is not known because the large-scale study is not conducted in this country. therefore, this study aimed to check the presence and distribution of arv-specific antibody in respect to area, types of chickens (broiler breeder, broiler, and layer), vaccination status, and age of chickens in gazipur and mymensingh ...201931528043
molecular identification of reovirus in broiler type flocks in golestan province, iran.avian reovirus (arv) has a global distribution in nature and most clinical signs are found in broiler type chickens.201931531032
rapid and sensitive real-time recombinase polymerase amplification for detection of marek's disease virus.marek's disease (md) is one of the most devastating diseases of poultry. it's caused by the highly infectious alphaherpesvirus md virus serotype 1 (mdv-1). in this study, a rapid and easy-to-use assay based on recombinase polymerase amplification (rpa) was developed for mdv detection. primer-probe sets targeting the highly conserved region of meq gene were designed and applied to the rpa assay. the assay was carried out on a real-time thermostatic fluorescence detector at 39 °c for 20 min. as re ...201931580913
the roles of micrornas (mirnas) in avian response to viral infection and pathogenesis of avian immunosuppressive diseases.micrornas (mirnas) are a class of non-coding small rnas that play important roles in the regulation of various biological processes including cell development and differentiation, apoptosis, tumorigenesis, immunoregulation and viral infections. avian immunosuppressive diseases refer to those avian diseases caused by pathogens that target and damage the immune organs or cells of the host, increasing susceptibility to other microbial infections and the risk of failure in subsequent vaccination aga ...201931683847
molecular detection of mycoplasma synoviae and avian reovirus infection in arthritis and tenosynovitis lesions of broiler and breeder chickens in santa catarina state, brazil.infectious arthritis or tenosynovitis in broiler and breeder chickens results in major loss of productivity because of reduced growth and downgrading at processing plants. the most common causative agents of avian infectious arthritis are the bacterium mycoplasma synoviae and avian reoviruses (arvs) (family reoviridae, genus orthoreovirus). in this study, we evaluated the occurrence of these two pathogens in arthritis or tenosynovitis lesions of broilers and breeder flocks in southern brazil usi ...201931793309
partial molecular characterization and pathogenicity study of an avian reovirus causing tenosynovitis in commercial broilers.this study describes the molecular characterization of avian reoviruses (arvs) isolated during an outbreak in commercial chickens between 2015 and 2016. in addition, a pathogenicity study of a selected arv strain isolated from a field case of viral tenosynovitis in commercial broiler chickens was performed. on the basis of phylogenetic analysis of a 1088-bp fragment of the arv s1 gene, the investigated sequences were differentiated into five distinct genotypic clusters (gcs), namely gc1, gc2, gc ...201931967428
changes in the percentages of b- and t-lymphocytes and antibody titres in laying hens infested with dermanyssus gallinae-a preliminary study.(1) background: dermanyssus gallinae, a hematophagous ectoparasite, adversely affects the health status of laying hens, leading to reduced egg production and significant economic losses in commercial farms. the aim of this study was to determine the effect of d. gallinae on the development of post-vaccination immune responses in layer hens. (2) methods: a total of 80 blood samples were collected at four time-points (b1-b4) from 10 hy-line brown hens, randomly selected from a commercial layer far ...202032517104
development and application of an mrt-qpcr assay for detecting coinfection of six vertically transmitted or immunosuppressive avian viruses.marek's disease virus (mdv), reticuloendotheliosis virus (rev), avian reovirus (arv), chicken infectious anemia virus (ciav), infectious bursal disease virus (ibdv), and fowl adenovirus (fadv) are important causes of disease in poultry. to investigate the infection status of the above six viruses in chickens in china, 1,187 samples from chicken flocks were collected and tested using a newly developed multiplex reverse-transcription quantitative real-time pcr (mrt-qpcr) assay in the study. a seri ...202032765453
seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of mymensingh district in bangladesh.the present study estimated the seroprevalence of avian reovirus (arv) infections in backyard chickens of the mymensingh district in bangladesh.202033005682
synergistic pathogenicity in sequential coinfection with fowl adenovirus type 4 and avian orthoreovirus.hydropericardium hepatitis syndrome (hhs) is a fatal disease caused by fowl adenovirus serotype 4 (fadv-4). avian viral arthritis is an infectious disease characterized by movement disorders caused by avian orthoreovirus (arv). in the early 2019, our epidemiologic survey on poultry diseases in eight commercial broiler farms in china showed that fadv-4 and arv have a high coinfection rate, accounting for 63 % of all arv-positive samples. we designed chicken embryo and animal models to investigate ...202033091795
novel cyclovirus identified in broiler chickens with transmissible viral proventriculitis in china.in october 2018, an outbreak of transmissible viral proventriculitis (tvp) occurred in 30-day-old commercial broiler chickens on a farm in weifang, china. tvp, an infectious viral disease characterized by runting and stunting, is associated with many viruses, and has a significant economic impact on the global poultry industry. tvp is diagnosed according to clinical symptoms, gross and histological lesions, and negative pcr results for pathogenic bacteria, avian leukosis virus subgroup j, marek' ...202033134354
establishment of a real-time recombinase polymerase amplification assay for the detection of avian reovirus.avian reovirus (arv) infection results in multiple disease manifestations in chicken. a rapid detection method will contribute to early diagnosis and control of the virus infection. the recombinase polymerase amplification (rpa) technology is a nucleic acid amplification method which is experiencing rapid development. in present study, a real-time reverse transcription (rt)-rpa assay was developed for the detection of arv. the limit of detection of the real-time rt-rpa was 102 copies/μl of arv g ...202033195523
gallus nme/nm23 nucleoside diphosphate kinase 2 interacts with viral σa and affects the replication of avian reovirus.our present study aimed to identify host cell proteins that may interact with avian reovirus (arv) σa protein and their potential effect on arv replication. the arv structural protein σa has been demonstrated to suppress interferon production and confirmed to activate the pi3k/akt pathway. however, host cell factors interacting with σa to affect arv replication remain unknown. in current study, a cdna library of chicken embryo fibroblasts (cefs) was constructed, and host cell proteins interactin ...202033223234
metagenomic characterisation of additional and novel avian viruses from australian wild ducks.birds, notably wild ducks, are reservoirs of pathogenic and zoonotic viruses such as influenza viruses and coronaviruses. in the current study, we used metagenomics to detect and characterise avian dna and rna viruses from wild pacific black ducks, chestnut teals and grey teals collected at different time points from a single location. we characterised a likely new species of duck aviadenovirus and a novel duck gyrovirus. we also report what, to the best of our knowledge, is the first finding of ...202033335272
emerging new avian reovirus variants from cases of enteric disorders and arthritis/tenosynovitis in brazilian poultry flocks.1. the objective of this study was to characterise circulating brazilian avian reovirus (arv) strains by genetic analysis of the σc protein encoded by segment 1 of the viral genome and compare these with those of viral strains used for immunising commercial poultry.2. the analysis detected the presence of arv genomes by quantitative reverse transcriptase pcr (rt-qpcr) in the enteric samples and the joint tissues (jt) of birds with signs of viral arthritis/tenosynovitis. nucleotide sequencing use ...202133448227
dynamic changes in the expression of interferon-stimulated genes in joints of spf chickens infected with avian reovirus.avian reovirus (arv) can induce many diseases as well as immunosuppression in chickens, severely endangering the poultry industry. interferons (ifns) play an antiviral role by inducing the expression of interferon-stimulated genes (isgs). the effect of arv infection on the expression of host isgs is unclear. specific-pathogen-free (spf) chickens were infected with arv strain s1133 in this study, and real time quantitative pcr was used to detect changes in the dynamic expression of ifns and commo ...202133614762
genomic characterization of avian and neoavian orthoreoviruses detected in pheasants.avian reoviruses are well-known pathogens seriously affecting the productivity of poultry industry. game birds represent a small segment of the agricultural sector and much remained to be learnt about factors affecting productivity. here we show that reovirus infections might occur in pheasants and demonstrate that reoviruses of pheasants are of diverse origin. the complete or coding-complete genomic sequences of two hungarian reovirus strains, d1996/2/1 and reo/hun/pheasant/216/2015, have been ...202133631220
detection and characterization of chicken astrovirus associated with hatchery disease in commercial day-old turkeys in southwestern nigeria.infectious diseases are a major obstacle to profitable poultry production in nigeria due to the mortality and severe economic losses they cause. in particular, they are a potent threat to attainment of the food security goals of government and national self-sufficiency in food production. thus, there is a need for continuous monitoring of the nation's poultry population for these diseases. as part of an ongoing investigation of enteric viruses associated with poor performance or hatchery disease ...202133759002
new insights on avian orthoreovirus and chicken astrovirus co-infection in an italian broiler flock: preliminary biomolecular and pathological results.common pathogens of intensive poultry farms, either parasitic or bacterial, such as coccidiaor salmonella, are well known and strictly controlled by veterinary management. this case study reports an unusual case of runting stunting syndrome (rss) observed on a sicilian poultry farm of broiler chickens during 2019. the investigation was carried out on five chickens which present delayed in body weight and growth performance. animals showed also difficulty in deambulation and diarrhea. at necropsy ...202134313103
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