Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| extrapulmonary infections caused by a dominant strain of mycobacterium massiliense (mycobacterium abscessus subspecies bolletii). | a single strain of mycobacterium massiliense (bra 100), a subspecies of the mycobacterium abscessus complex, has been responsible for an epidemic of post-surgical infections in brazil. outside brazil, this is the first report to describe a single emerging strain of m. massiliense (tpe 101) associated with extrapulmonary infections. this phenomenon may be underestimated because sophisticated molecular typing of m. abscessus is not routinely performed. our molecular epidemiology study was triggere ... | 2013 | 23718188 |
| catheter related infection due to mycobacterium abscessus in a patient under peritoneal dialysis. | 2013 | 23735153 | |
| emergence of clinically relevant non-tuberculous mycobacterial infections in saudi arabia. | non-tuberculous mycobacteria (ntm) are emerging around the world due to a higher prevalence of immunosuppressive illness and therapy. saudi arabia is not an exception as there have been novel mycobacterial species also identified. in addition, several published case reports from different parts of the country suggest a growing pathogenic potential of ntm. as the first nationwide study, we sought to gain an insight into the species diversity of ntm clinical isolates. | 2013 | 23738024 |
| serodiagnosis of mycobacterium avium complex and mycobacterium abscessus complex pulmonary disease by use of iga antibodies to glycopeptidolipid core antigen. | an enzyme immunoassay kit that detects serum iga antibody reacting to glycopeptidolipid core antigen derived from mycobacterium avium complex (mac) was not useful for differentiating mac pulmonary disease (pd) from mycobacterium abscessus complex pd (mab-pd). however, this assay could be useful for differentiating mac- and mab-pd from pulmonary tuberculosis. (this study has been registered at clinicaltrials.gov under registration no. nct00970801.). | 2013 | 23740728 |
| adherence and biofilm formation of mycobacterium avium, mycobacterium intracellulare and mycobacterium abscessus to household plumbing materials. | measure adherence and biofilm formation by cells of mycobacterium avium, mycobacterium intracellulare and mycobacterium abscessus on common household plumbing materials namely stainless steel, glass, zinc-galvanized steel, copper and polyvinyl chloride (pvc). | 2013 | 23742161 |
| b-cell lymphoma in a patient with complete interferon gamma receptor 1 deficiency. | immunosuppression-associated lymphoproliferative disorders can be related to primary as well as acquired immune disorders. interferon gamma receptor (ifn-γr) deficiency is a rare primary immune disorder, characterized by increased susceptibility to mycobacterial infections. here we report the first case of an epstein barr virus (ebv) related b-cell lymphoma in a patient with complete ifn-γr1 deficiency. the patient was a 20-year-old man with homozygous 22cdel in ifngr1 resulting in complete abse ... | 2013 | 23800860 |
| new rapid scheme for distinguishing the subspecies of the mycobacterium abscessus group and identifying mycobacterium massiliense isolates with inducible clarithromycin resistance. | mycobacterium abscessus (m. abscessus sensu lato, or the m. abscessus group) comprises three closely related taxa whose taxonomic statuses are under revision, i.e., m. abscessus sensu stricto, mycobacterium bolletii, and mycobacterium massiliense. we describe here a simple, robust, and cost-effective pcr-based method for distinguishing among m. abscessus, m. massiliense, and m. bolletii. based on the m. abscessus atcc 19977(t) genome, regions that discriminated between m. abscessus and m. massil ... | 2013 | 23804391 |
| recent advances in tuberculosis and nontuberculous mycobacteria lung disease. | tuberculosis (tb) is one of the largest health problems in the world today. and the incidence of nontuberculous mycobacteria (ntm) lung disease appears to be increasing worldwide. recently, an automated, nucleic acid amplification assay for the rapid detection of both mycobacterium tuberculosis and rifampin resistance was developed (xpert mtb/rif). and fixed-dose combinations of anti-tb drugs and linezolid have been introduced in the treatment of tb. and new ntm species, named mycobacterium mass ... | 2013 | 23814596 |
| lung transplantation for cystic fibrosis and bronchiectasis. | lung transplantation has become an excellent treatment option for patients with cystic fibrosis (cf) and bronchiectasis with very advanced lung disease. despite the challenges that the cf patients present, survival is more favorable than that seen in patients with chronic obstructive pulmonary disease and pulmonary fibrosis. although those cf and bronchiectasis patients with severe respiratory disease are often infected with organisms that display in vitro resistance to the commonly used antibio ... | 2013 | 23821505 |
| mycobacterium abscessus bacteremia after receipt of intravenous infusate of cytokine-induced killer cell therapy for body beautification and health boosting. | we report the first series of mycobacterium abscessus bacteremia after cytokine-induced killer cell therapy for body beautification and health boosting. | 2013 | 23825355 |
| nontuberculous mycobacteria in respiratory samples from patients with pulmonary tuberculosis in the state of rondônia, brazil. | the main cause of pulmonary tuberculosis (tb) is infection with mycobacterium tuberculosis (mtb). we aimed to evaluate the contribution of nontuberculous mycobacteria (ntm) to pulmonary disease in patients from the state of rondônia using respiratory samples and epidemiological data from tb cases. mycobacterium isolates were identified using a combination of conventional tests, polymerase chain reaction-based restriction enzyme analysis of hsp65 gene and hsp65 gene sequencing. among the 1,812 ca ... | 2013 | 23827995 |
| thoracic empyema and bacteremia due to mycobacterium abscessus in a patient with liver cirrhosis. | empyema thoracis with concomitant bacteremia caused by nontuberculous mycobacterium is rare. herein, we report a case of disseminated mycobacterium abscessus in a patient with liver cirrhosis and diabetes mellitus. m. abscessus was isolated from the specimen of pleural fluid and blood. the patients clinical condition gradually improved after antibiotic use and drainage. | 2013 | 23831159 |
| drosophila melanogaster model for mycobacterium abscessus infection. | mycobacterium abscessus is a human pathogen that is responsible for a broad spectrum of tissue infections and disseminated infections in immunodeficient patients. this pathogen is one of the most resistant organisms to chemotherapeutic agents. therefore, we tested the hypothesis that the fruit fly, drosophila melanogaster, is a genetically tractable model host for m. abscessus. in this context, we infected d. melanogaster with m. abscessus. this m. abscessus infection results in dissemination in ... | 2013 | 23831804 |
| complete genome sequence of mycobacterium massiliense clinical strain asan 50594, belonging to the type ii genotype. | we report the complete genome sequence of the mycobacterium massiliense clinical strain asan 50594, which was grouped into the m. massiliense type ii genotype, isolated from a korean patient. this genome sequence will serve as a valuable reference for understanding the disparity in virulence and epidemiological traits between strains belonging to the mycobacterium abscessus complex. | 2013 | 23833135 |
| potentiation of high hydrostatic pressure inactivation of mycobacterium by combination with physical and chemical conditions. | mycobacterium abscessus is an important hospital-acquired pathogen involved in infections associated with medical, surgical, and biopharmaceutical materials. in this work, we investigated the pressure-induced inactivation of two strains [2544 and american type culture collection (atcc) 19977] of m. abscessus in combination with different temperatures and ph conditions. for strain 2544, exposure to 250 mpa for 90 min did not significantly inactivate the bacteria at 20 °c, whereas at -15 °c, there ... | 2013 | 23836348 |
| mab_3168c, a putative acetyltransferase, enhances adherence, intracellular survival and antimicrobial resistance of mycobacterium abscessus. | mycobacterium abscessus is a non-tuberculous mycobacterium. it can cause diseases in both immunosuppressed and immunocompetent patients and is highly resistant to multiple antimicrobial agents. m. abscessus displays two different colony morphology types: smooth and rough morphotypes. cells with a rough morphotype are more virulent. the purpose of this study was to identify genes responsible for m. abscessus morphotype switching. with transposon mutagenesis, a mutant with a tn5 inserted into the ... | 2013 | 23840740 |
| further isolation of mycobacterium abscessus subsp. abscessus and subsp. bolletii in different regions of japan and susceptibility of these isolates to antimicrobial agents. | the aim of this study was to genetically analyse mycobacterium abscessus subsp. abscessus (hereafter m. abscessus) and m. abscessus subsp. bolletii (hereafter m. bolletii) isolates from six different regions of japan and to determine the antimicrobial susceptibility of these isolates. subspeciation of 143 clinical isolates of m. abscessus group was done by comparative sequence analysis of the rpob and hsp65 genes and the internal transcribed spacer (its) region. genetic analysis led to the ident ... | 2013 | 23850022 |
| mycobacterium abscessus peritonitis associated with laparoscopic gastric banding. | mycobacterium abscessus is a rapidly growing mycobacterium that is a common water contaminant in the environment. we report a case of m. abscessus infection with band erosion following laparoscopic gastric banding. | 2013 | 23855515 |
| selected insights from application of whole-genome sequencing for outbreak investigations. | the advent of high-throughput whole-genome sequencing has the potential to revolutionize the conduct of outbreak investigation. because of its ultimate resolution power for differentiating between closely related pathogen strains, whole-genome sequencing could augment the traditional epidemiologic investigations of infectious disease outbreaks. | 2013 | 23856896 |
| standardization of multilocus sequence typing scheme for mycobacterium abscessus and mycobacterium massiliense. | this study aims to develop a multilocus sequence typing (mlst) scheme for mycobacterium abscessus complex for the typing of stains within each species. a total of 89 clinical isolates of m. abscessus complex from 71 patients of 2 tertiary care hospitals in south korea were included. forty-two isolates were identified as m. abscessus, and 29, as mycobacterium massiliense through sequencing of 8 housekeeping genes and rpob. the mlst scheme identified 26 different sequence types(sts) and 13 differe ... | 2013 | 23891467 |
| vertebral osteomyelitis caused by mycobacterium abscessus. | mycobacterium infection caused by non-tuberculous mycobacterial (ntbm) organisms is becoming more common. although ntbm osteomyelitis is unusual, it can occur in otherwise healthy individuals, but it is also associated with immunocompromised states, such as steroidal therapy and aids, and may be observed following trauma. mycobacterium avium is reported to be the most common causative agent, and mycobacterium abscessus has only been described in two cases. we report the case of a 44-year-old man ... | 2013 | 23925676 |
| mixed mycobacterial infection in an adult koi carp cyprinus carpio l. | 2014 | 23937602 | |
| in vitro activity of amikacin against isolates of mycobacterium avium complex with proposed mic breakpoints and finding of a 16s rrna gene mutation in treated isolates. | amikacin is a major drug used for the treatment of mycobacterium avium complex (mac) disease, but standard laboratory guidelines for susceptibility testing are not available. this study presents in vitro amikacin mics for 462 consecutive clinical isolates of the mac using a broth microdilution assay. approximately 50% of isolates had amikacin mics of 8 μg/ml, and 86% had mics of ≤16 μg/ml. of the eight isolates (1.7%) with mics of 64 μg/ml, five had an mic of 32 μg/ml on repeat testing. ten isol ... | 2013 | 23946523 |
| normalization of elevated ca 19-9 level after treatment in a patient with the nodular bronchiectatic form of mycobacterium abscessus lung disease. | carbohydrate antigen 19-9 (ca 19-9) is a widely-used tumor marker in patients with pancreatic cancer. however, some patients with respiratory disease also exhibit elevated serum ca 19-9 levels. we report a case of normalization of elevated serum ca 19-9 levels after treatment of the nodular bronchiectatic form of mycobacterium ab scessus lung disease. a 40-year-old man visited our hospital because of chronic cough and sputum. a computed tomography scan revealed severe bronchiectasis in the right ... | 2013 | 23946755 |
| genome sequence of an epidemic isolate of mycobacterium abscessus subsp. bolletii from rio de janeiro, brazil. | multiple isolates of mycobacterium abscessus subsp. bolletii, collectively called bra100, were associated with outbreaks of postsurgical skin infections across various regions of brazil from 2003 to 2009. we announce the draft genome sequence of a newly sequenced bra100 strain, m. abscessus subsp. bolletii crm-0020, isolated from a patient in rio de janeiro, brazil. | 2013 | 23950125 |
| non-tuberculous mycobacteria: a retrospective review of scottish isolates from 2000 to 2010. | there is growing recognition of the clinical importance of non-tuberculous mycobacteria (ntm), a group of versatile opportunistic bacterial pathogens. we describe the characteristics of ntm isolates in scotland over an 11-year period using data held by the scottish mycobacteria reference laboratory. american thoracic society microbiological criteria were used to evaluate the clinical significance of isolates. data presented include analysis of trends across time, species/body site associations, ... | 2014 | 23986391 |
| simultaneous detection of mycobacterium tuberculosis complex and nontuberculous mycobacteria in respiratory specimens. | many nontuberculous mycobacteria (ntm) species have clinical significance, and the rapid and reliable identification of mycobacterium tuberculosis complex (mtbc) and ntm species is important. we evaluated the simultaneous detection of mtbc and ntm in respiratory specimens. mtbc and ntm were simultaneously detected and identified by laboratory-developed (ldt) real-time pcr, multiplex real-time pcr/melting curve analysis, rpob pcr restriction fragment length polymorphisms and the advansure mycobac ... | 2013 | 23988279 |
| identification and characterization of the genetic changes responsible for the characteristic smooth-to-rough morphotype alterations of clinically persistent mycobacterium abscessus. | mycobacterium abscessus is an emerging pathogen that is increasingly recognized as a relevant cause of human lung infection in cystic fibrosis patients. this highly antibiotic-resistant mycobacterium is an exception within the rapidly growing mycobacteria, which are mainly saprophytic and non-pathogenic organisms. m. abscessus manifests as either a smooth (s) or a rough (r) colony morphotype, which is of clinical importance as r morphotypes are associated with more severe and persistent infectio ... | 2013 | 23998761 |
| post-operative sinus formation due to mycobacterium abscessus: a case report. | mycobacterium abscessus is ubiquitously found rapidly growing mycobacteria. although it is an uncommon pathogen, it has been known to cause cutaneous infection following inoculation, minor trauma or surgery. this communication reports an immuno-competent patient developing multiple sinuses due to mycobacterium abscessus in the post- operative period. | 2013 | 24000496 |
| whole-genome sequence of mycobacterium abscessus clinical strain v06705. | infection caused by mycobacterium abscessus strains is a growing cause of concern in both community-acquired and health care-associated diseases, as these organisms naturally display multiple drug resistances. we report an annotated draft genome sequence of m. abscessus strain v06705 obtained from a patient in france. | 2013 | 24009121 |
| insertion site and distribution of a genomic island conferring dna phosphorothioation in the mycobacterium abscessus complex. | nearly half of us clinical isolates of the emerging pathogen mycobacterium abscessus were reported to exhibit smeared dna during pfge. this dna degradation (dnd) phenotype results from dna phosphorothioation, a sulfur modification found in other bacteria and conferred by dnd genes located on mobile elements. putative dnd genes are located on a 19.6 kbp genomic island (gi) in the m. abscessus type strain atcc 19977. we confirmed that atcc 19977(t) is dnd-positive by pfge and we developed a pcr as ... | 2013 | 24014661 |
| a case of erosive polyarthritis in a patient diagnosed with a suspicion of atypical mycobacteria. | in this report, we introduce a case of erosive polyarthritis in a 55-year-old female diagnosed with mycobacterium abscessus pulmonary infection. her arthritis has been worsened after use of dmards. the patient demonstrated a significant response to the antimicrobial regimen that was administered. we call special attention to the possibility of mycobacterium abscessus being a cause of reactive polyarthritis, particularly if symptoms worsened after use of disease-modifying antirheumatic drugs (dma ... | 2013 | 24039641 |
| an outbreak of mycobacterium abscessus infection in a pediatric intensive care unit in kuwait. | mycobacterium abscessus has been associated with respiratory tract infections, localized skin and soft tissue infections and sepsis. however, outbreaks of m. abscessus are rare. | 2014 | 24042493 |
| nontuberculous mycobacteria: the changing epidemiology and treatment challenges in cystic fibrosis. | although patients with cystic fibrosis (cf) face numerous infectious pathogens over the course of their lifespan, increasing attention has recently been paid to nontuberculous mycobacteria (ntm). as reported prevalence rates rise across many countries such as the united states, the ability to recognize disease caused by ntm and subsequently treat such disease has become increasingly important. this review summarizes new observations on the epidemiology of ntm in cf as well as key elements to con ... | 2013 | 24048085 |
| advantages of using matrix-assisted laser desorption ionization-time of flight mass spectrometry as a rapid diagnostic tool for identification of yeasts and mycobacteria in the clinical microbiological laboratory. | yeast and mycobacteria can cause infections in immunocompromised patients and normal hosts. the rapid identification of these organisms can significantly improve patient care. there has been an increasing number of studies on using matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) for rapid yeast and mycobacterial identifications. however, studies on direct comparisons between the bruker biotyper and biomérieux vitek ms systems for the identification of ... | 2013 | 24048537 |
| phylogenomics of brazilian epidemic isolates of mycobacterium abscessus subsp. bolletii reveals relationships of global outbreak strains. | rapidly growing, non-tuberculous mycobacteria (ntm) in the mycobacterium abscessus (mab) species are emerging pathogens that cause various diseases including skin and respiratory infections. the species has undergone recent taxonomic nomenclature refinement, and is currently recognized as two subspecies, m. abscessus subsp. abscessus (mab-a) and m. abscessus subsp. bolletii (mab-b). the recently reported outbreaks of mab-b in surgical patients in brazil from 2004 to 2009 and in cystic fibrosis p ... | 2013 | 24055961 |
| in vitro activity of cefoxitin and imipenem against mycobacterium abscessus complex. | the in vitro activity of cefoxitin and imipenem was compared for 43 strains of the mycobacterium abscessus complex, mostly isolated from cystic fibrosis patients. the mics of imipenem were lower than those of cefoxitin, although the number of imipenem-resistant strains was higher according to the clsi breakpoints. strain comparisons indicated that the mics of cefoxitin were significantly higher for mycobacterium bolletii than for m. abscessus. the mics of both β-lactams were higher for the rough ... | 2014 | 24112243 |
| correction: the detection and sequencing of a broad-host-range conjugative incp-1β plasmid in an epidemic strain of mycobacterium abscessus subsp. bolletii. | [this corrects the article on p. e60746 in vol. 8.]. | 2013 | 24116241 |
| drug treatment of pulmonary nontuberculous mycobacterial disease in hiv-negative patients: the evidence. | pulmonary disease (pd) caused by nontuberculous mycobacteria is an emerging infection mainly in countries where the incidence of tuberculosis is in decline. it affects an elderly population, often with underlying chronic lung diseases, but its epidemiology shows significant regional variation. guidelines and recommendations for treatment of these infections exist, but build strongly on expert opinion, as very few good quality clinical trials have been performed in this field. only for the most f ... | 2013 | 24124798 |
| characterization of broad-spectrum mycobacterium abscessus class a β-lactamase. | imipenem and cefoxitin are used to treat mycobacterium abscessus infections and have moderate activity against this fast-growing mycobacterium (mic₅₀ of 16 and 32 mg/l, respectively). m. abscessus is highly resistant to most other β-lactams, although the underlying mechanisms have not been explored. here, we characterized m. abscessus class a β-lactamase (bla(mab)) and investigated its role in β-lactam resistance. | 2014 | 24132992 |
| mycobacterium abscessus scleritis following intravitreous injection of bevacizumab. | 2013 | 24151651 | |
| discrimination of mycobacterium abscessus subsp. massiliense from mycobacterium abscessus subsp. abscessus in clinical isolates by multiplex pcr. | the rapidly growing mycobacterium m. abscessus sensu lato is the causative agent of emerging pulmonary and skin diseases and of infections following cosmetic surgery and postsurgical procedures. m. abscessus sensu lato can be divided into at least three subspecies: m. abscessus subsp. abscessus, m. abscessus subsp. massiliense, and m. abscessus subsp. bolletii. clinical isolates of rapidly growing mycobacteria were previously identified as m. abscessus by dna-dna hybridization. more than 30% of ... | 2014 | 24197885 |
| characterization of rough and smooth morphotypes of mycobacterium abscessus isolates from clinical specimens. | mycobacterium abscessus, which consists of the two subspecies m. abscessus subspecies abscessus and m. abscessus subspecies bolletii, can produce rough or smooth colony morphologies. here we analyzed 50 m. abscessus isolates cultured from the respiratory specimens of 34 patients, 28 (82%) of whom had cystic fibrosis (cf), with respect to their colony morphologies and antibiotic susceptibilities. the overall proportions of occurrences of the two morphotypes were similar, with specimens from 50% o ... | 2014 | 24197890 |
| draft genome sequence of mycobacterium abscessus subsp. bolletii incqs 00594. | an epidemic of surgical-site infections by a single strain of mycobacterium abscessus subsp. bolletii affected >1,700 patients in brazil from 2004 to 2008. the genome of the epidemic prototype strain m. abscessus subsp. bolletii incqs 00594, deposited in the collection of the national institute for health quality control (incqs), was sequenced. | 2013 | 24201191 |
| gm-csf knockout mice for preclinical testing of agents with antimicrobial activity against mycobacterium abscessus. | of the non-tuberculous mycobacteria, mycobacterium abscessus is particularly refractory to antimicrobial therapy and new agents with activity against these pathogens are urgently needed. the screening of candidate antimicrobial agents against m. abscessus requires a relevant and reproducible animal model of chronic infection. granulocyte-macrophage colony-stimulating factor knockout (gm-csf ko) mice were used to develop a new animal model of chronic pulmonary m. abscessus infection that can be u ... | 2014 | 24222613 |
| [drug sensitivity analysis of mycobacterium chelonae and mycobacterium abscessus and evaluation of etest for susceptibility testing]. | to study the drug resistance profile of mycobacterium(m.) chelonae and m.abscessus and to evaluate the clinical application of etest(epsilometer test) for susceptibility testing. | 2013 | 24252731 |
| in vivo evaluation of antibiotic activity against mycobacterium abscessus. | the prognosis of mycobacterium abscessus infections is poor due to the lack of effective drug treatment. the objective of this study was to set up an animal model suitable to test antibiotic activity against m. abscessus. | 2014 | 24253289 |
| bronchopleural fistula following m. abscessus infection 11 years after lobectomy for lung cancer. | bronchopleural fistula (bpf) is a potentially fatal complication of lung cancer resection surgery that occurs during the healing process of the bronchial stump. however, the vulnerability of the healed surgical wound to overlapping acquired airway destruction has not yet been determined in detail. we herein present a case of fatal bpf following mycobacterium abscessus (m. abscessus) infection, which occurred 11 years after right upper lobectomy for lung cancer. the findings of the present study ... | 2013 | 24255862 |
| sporotrichoid cutaneous infection by mycobacterium abscessus. | 2014 | 24261556 | |
| bacterial adherence to different meshes used in abdominal surgery. | we studied the influence of morphology and type of material of abdominal wall prostheses in the avoidance of bacterial adhesion in acute and chronic mesh infections. | 2014 | 24283764 |
| the genome sequence of 'mycobacterium massiliense' strain cip 108297 suggests the independent taxonomic status of the mycobacterium abscessus complex at the subspecies level. | members of the mycobacterium abscessus complex are rapidly growing mycobacteria that are emerging as human pathogens. the m. abscessus complex was previously composed of three species, namely m. abscessus sensu stricto, 'm. massiliense', and 'm. bolletii'. in 2011, 'm. massiliense' and 'm. bolletii' were united and reclassified as a single subspecies within m. abscessus: m. abscessus subsp. bolletii. however, the placement of 'm. massiliense' within the boundary of m. abscessus subsp. bolletii r ... | 2013 | 24312320 |
| heterologous expression of mycobacterial esx complexes in escherichia coli for structural studies is facilitated by the use of maltose binding protein fusions. | the expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. the reason is that the solubility and proper folding of each subunit of the complex requires physical association with other subunits of the complex. the genomes of pathogenic mycobacteria encode many small protein complexes, implicated in bacterial fitness and pathogenicity, whose characterization may be further complicated by insolubility upon expression in escherichia col ... | 2013 | 24312350 |
| a phylogenomic approach to bacterial subspecies classification: proof of concept in mycobacterium abscessus. | mycobacterium abscessus is a rapidly growing mycobacterium that is often associated with human infections. the taxonomy of this species has undergone several revisions and is still being debated. in this study, we sequenced the genomes of 12 m. abscessus strains and used phylogenomic analysis to perform subspecies classification. | 2013 | 24330254 |
| rough colony morphology of mycobacterium massiliense type ii genotype is due to the deletion of glycopeptidolipid locus within its genome. | recently, we introduced the complete genome sequence of mycobacterium massiliense clinical isolates, asan 50594 belonging to type ii genotype with rough colony morphology. here, to address the issue of whether the rough colony morphotype of m. massiliense type ii genotype is genetically determined or not, we compared polymorphisms of the glycopeptidolipid (gpl) gene locus between m. massiliense type ii asan 50594 and other rapidly growing mycobacteria (rgm) strains via analysis of genome databas ... | 2013 | 24341808 |
| increasing recovery of nontuberculous mycobacteria from respiratory specimens over a 10-year period in a tertiary referral hospital in south korea. | the number of patients with pulmonary disease caused by nontuberculous mycobacteria (ntm) has been increasing worldwide. the aim of this study was to evaluate long-term trends in the ntm recovery rate from respiratory specimens over a 10-year period in a tertiary referral hospital in south korea. | 2013 | 24348667 |
| risk factors for nontuberculous mycobacterial infections in solid organ transplant recipients: a case-control study. | the epidemiology of nontuberculous mycobacteria (ntm) disease in solid organ transplant recipients is poorly defined. | 2014 | 24350627 |
| multilocus sequence typing scheme for the mycobacterium abscessus complex. | we developed a multilocus sequence typing (mlst) scheme for mycobacterium abscessus sensu lato, based on the partial sequencing of seven housekeeping genes: argh, cya, glpk, gnd, murc, pta and purh. this scheme was used to characterize a collection of 227 isolates recovered between 1994 and 2010 in france, germany, switzerland and brazil. we identified 100 different sequence types (sts), which were distributed into three groups on the tree obtained by concatenating the sequences of the seven hou ... | 2014 | 24384536 |
| antimicrobial susceptibility of standard strains of nontuberculous mycobacteria by microplate alamar blue assay. | in this study, 24 standard nontuberculous mycobacteria (ntm) species strains including 12 slowly growing mycobacteria strains and 12 rapidly growing mycobacteria strains were subjected to drug susceptibility testing using microplate alamar blue assay-based 7h9 broth. the most active antimicrobial agents against the 24 ntm strains were streptomycin, amikacin, the fluoroquinolones, and the tetracyclines. mycobacterium chelonae, mycobacterium abscessus, mycobacterium bolletii, and mycobacterium sim ... | 2013 | 24386332 |
| recent progress towards understanding genetic variation in the mycobacterium abscessus complex. | mycobacterium abscessus is an emerging cause of respiratory disease and soft tissue infections. whole genome sequencing and other molecular approaches are enhancing our understanding of outbreaks, antibiotic resistance mechanisms, and virulence properties, and of the phylogeny of the m. abscessus complex. infection models are providing further insights into factors such as colony phenotype that impact host-pathogen interactions. this paper reviews recent developments in our understanding of gene ... | 2013 | 24388643 |
| the drug resistance profile of mycobacterium abscessus group strains from korea. | bacteria of the mycobacterium abscessus group are the second most common pathogens responsible for lung disease caused by nontuberculous mycobacteria in korea. there is still a lack of studies investigating the genetic mechanisms involved in m. abscessus resistance to antibiotics other than clarithromycin. this study investigated the characteristics of drug resistance exhibited by m. abscessus clinical isolates from korea. | 2014 | 24422193 |
| [mycobacterium abscessus group lung disease: case reports and review of the literature]. | to analyze the clinical manifestations and efficacy of a combination antibiotic therapy including cefoxitin for mycobacterium abscessus (m.abscessus) group lung disease. | 2013 | 24423821 |
| the cystic fibrosis lung microbiome. | the chronic colonization of the lower airways by bacterial pathogens is the leading cause of morbidity and mortality in patients with cystic fibrosis (cf). pseudomonas aeruginosa is the most common cf pathogen, followed by staphylococcus aureus. improvements in airway clearance and more effective treatment of the conventional cf pathogens has led to the emergence of new airway pathogens such as stenotrophomonas maltophilia, mycobacterium abscessus, and achromobacter. more recently, it has become ... | 2014 | 24437409 |
| inhaled amikacin for treatment of refractory pulmonary nontuberculous mycobacterial disease. | treatment of pulmonary nontuberculous mycobacteria, especially mycobacterium abscessus, requires prolonged, multidrug regimens with high toxicity and suboptimal efficacy. options for refractory disease are limited. | 2014 | 24460437 |
| managing pulmonary nontuberculous mycobacterial infection. time for a patient-centered approach. | the incidence of nontuberculous mycobacteria is increasing worldwide. however, the evidence base for clinical management comprises mostly expert opinion, case series, and few randomized clinical trials. most currently recommended treatment regimens entail prolonged use of multiple antimicrobial agents associated with multiple self-limited and persistent potential adverse effects, including irreversible impairments of hearing, vision, and kidney function. yet, little is known about how treatment ... | 2014 | 24460445 |
| respiratory infections in patients with cystic fibrosis undergoing lung transplantation. | cystic fibrosis is an inherited disease characterised by chronic respiratory infections associated with bronchiectasis. lung transplantation has helped to extend the lives of patients with cystic fibrosis who have advanced lung disease. however, persistent, recurrent, and newly acquired infections can be problematic. classic cystic fibrosis-associated organisms, such as staphylococcus aureus and pseudomonas aeruginosa, are generally manageable post-transplantation, and are associated with favour ... | 2014 | 24461904 |
| sacroiliitis secondary to catheter-related bacteremia due to mycobacterium abscessus (sensu stricto). | we describe a case of sacroiliitis secondary to catheter-related bacteremia due to mycobacterium abscessus (sensu stricto). this case confirms that multilocus sequence typing and variable-number tandem-repeat methods are very robust techniques to identify the pathogen species and to validate molecular epidemiological links among complex m. abscessus isolates. | 2014 | 24479655 |
| [a clinical study of pulmonary mycobacterium abscessus infection]. | pulmonary mycobacterium abscessus infection is resistant to many antibiotics and is difficult to treat. we retrospectively analyzed the clinical characteristics of pulmonary infection due to m. abscessus. eleven cases diagnosed as having pulmonary m. abscessus infection at osaka red cross hospital from january. 2008, to june, 2012 were enrolled in this study. the average age of the 11 cases was 63 years (all were females). nine cases showed underlying diseases, comprising 5 cases with mycobacter ... | 2013 | 24483019 |
| nontuberculous mycobacterial (ntm) lung disease: the top ten essentials. | this review will utilize essential questions about nontuberculous mycobacterial (ntm) lung disease to succinctly address important new developments in the pathogenesis, diagnosis and management of ntm lung disease with a focus on practical information and "bottom line" answers. 1) what do i tell my patients who ask, “where did i get this infection” and, “should i take showers”? 2) what is the connection between bronchiectasis and the acquisition of ntm lung infection? 3) what other factors are i ... | 2014 | 24484653 |
| contamination of hypertonic saline solutions in use by cystic fibrosis patients in israel. | treatment of cystic fibrosis (cf) patients with inhaled hypertonic saline (hs) solutions is safe, beneficial and reduces exacerbation rates. we studied contamination of solutions used by israeli cf patients for prolonged periods. | 2014 | 24484849 |
| erm(41)-dependent inducible resistance to azithromycin and clarithromycin in clinical isolates of mycobacterium abscessus. | the ribosomal methylase erm(41) confers inducible resistance to macrolides in mycobacterium abscessus. the aim of this work was to systematically study and compare drug susceptibility to clarithromycin and azithromycin in m. abscessus and mycobacterium chelonae clinical isolates with a particular focus on inducible drug resistance. | 2014 | 24500188 |
| genomic reconnaissance of clinical isolates of emerging human pathogen mycobacterium abscessus reveals high evolutionary potential. | mycobacterium abscessus (ma) is an emerging human pathogen that causes both soft tissue infections and systemic disease. we present the first comparative whole-genome study of ma strains isolated from patients of wide geographical origin. we found a high proportion of accessory strain-specific genes indicating an open, non-conservative pan-genome structure, and clear evidence of rapid phage-mediated evolution. although we found fewer virulence factors in ma compared to m. tuberculosis, our data ... | 2014 | 24515248 |
| the clinical management of cesarean section-acquired mycobacterium abscessus surgical site infections. | rapidly growing mycobacteria (rgm) can cause a broad spectrum of both community and healthcare-associated infections in humans. the aim of this study was to report the clinical management and outcomes of successive patients following cesarean delivery with healthcare-associated surgical site infections (ssis) caused by rgm. | 2014 | 24518628 |
| novel drug combination for mycobacterium abscessus disease therapy identified in a drosophila infection model. | mycobacterium abscessus is known to be the most drug-resistant mycobacterium and accounts for ∼80% of pulmonary infections caused by rapidly growing mycobacteria. this study reports a new drosophila melanogaster-m. abscessus infection model that can be used as an in vivo efficacy model for anti-m. abscessus drug potency assessment. | 2014 | 24519481 |
| the first korean case of nontuberculous mycobacterial lung disease caused by mycobacterium abscessus subspecies bolletii in a patient with bronchiectasis. | we report the first korean case of lung diseases caused by mycobacterium abscessus subsp. bolletii in a previously healthy male, except for a previous history of pulmonary tuberculosis and bronchiectasis. all serial isolates are identified as m. abscessus subsp. bolletii by multi-locus sequence analysis based on the hsp65, rpob, and 16s rrna fragments. at the genetic level, the isolate has the erm(41) gene with a t28 sequevar, associated with clarithromycin resistance, and no rrl mutation. the i ... | 2014 | 24523815 |
| rapid molecular detection of inducible macrolide resistance in mycobacterium chelonae and m. abscessus strains: a replacement for 14-day susceptibility testing? | the erm(41) gene causes inducible macrolide resistance in mycobacterium abscessus but not mycobacterium chelonae. erm(41) sequencing of 285 m. abscessus and 45 m. chelonae isolates was compared to 14-day susceptibility; agreement percentages were 98.9% and 100%, respectively. extended incubation may not be necessary for m. chelonae, and the erm(41) genotype is a useful adjunct for m. abscessus. | 2014 | 24554745 |
| high-level relatedness among mycobacterium abscessus subsp. massiliense strains from widely separated outbreaks. | three recently sequenced strains isolated from patients during an outbreak of mycobacterium abscessus subsp. massiliense infections at a cystic fibrosis center in the united states were compared with 6 strains from an outbreak at a cystic fibrosis center in the united kingdom and worldwide strains. strains from the 2 cystic fibrosis outbreaks showed high-level relatedness with each other and major-level relatedness with strains that caused soft tissue infections during an epidemic in brazil. we ... | 2014 | 24565502 |
| mycobacterium abscessus cording prevents phagocytosis and promotes abscess formation. | mycobacterium abscessus is a rapidly growing mycobacterium causing a wide spectrum of clinical syndromes. it now is recognized as a pulmonary pathogen to which cystic fibrosis patients have a particular susceptibility. the m. abscessus rough (r) variant, devoid of cell-surface glycopeptidolipids (gpls), causes more severe clinical disease than the smooth (s) variant, but the underlying mechanisms of r-variant virulence remain obscure. exploiting the optical transparency of zebrafish embryos, we ... | 2014 | 24567393 |
| acetic acid, the active component of vinegar, is an effective tuberculocidal disinfectant. | effective and economical mycobactericidal disinfectants are needed to kill both mycobacterium tuberculosis and non-m. tuberculosis mycobacteria. we found that acetic acid (vinegar) efficiently kills m. tuberculosis after 30 min of exposure to a 6% acetic acid solution. the activity is not due to ph alone, and propionic acid also appears to be bactericidal. m. bolletii and m. massiliense nontuberculous mycobacteria were more resistant, although a 30-min exposure to 10% acetic acid resulted in at ... | 2014 | 24570366 |
| demonstration of plasmid-mediated drug resistance in mycobacterium abscessus. | plasmid-mediated kanamycin resistance was detected in a strain of mycobacterium abscessus subsp. bolletii responsible for a nationwide epidemic of surgical infections in brazil. the plasmid did not influence susceptibility to tobramycin, streptomycin, trimethoprim-sulfamethoxazole, clarithromycin, or ciprofloxacin. plasmid-mediated drug resistance has not been described so far in mycobacteria. | 2014 | 24574286 |
| notes from the field: rapidly growing nontuberculous mycobacterium wound infections among medical tourists undergoing cosmetic surgeries in the dominican republic--multiple states, march 2013-february 2014. | in august 2013, the maryland department of health and mental hygiene (mdhmh) was notified of two persons with rapidly growing nontuberculous mycobacterial (rg-ntm) surgical-site infections. both patients had undergone surgical procedures as medical tourists at the same private surgical clinic (clinic a) in the dominican republic the previous month. within 7 days of returning to the united states, both sought care for symptoms that included surgical wound abscesses, clear fluid drainage, pain, an ... | 2014 | 24598597 |
| nontuberculous mycobacterial pulmonary infections. | pulmonary infections due to nontuberculous mycobacteria (ntm) are increasingly recognized worldwide. although over 150 different species of ntm have been described, pulmonary infections are most commonly due to mycobacterium avium complex (mac), mycobacterium kansasii, and mycobacterium abscessus. the identification of these organisms in pulmonary specimens does not always equate with active infection; supportive radiographic and clinical findings are needed to establish the diagnosis. it is dif ... | 2014 | 24624285 |
| a case of mycobacterium abscessus infection presenting as a cystic lesion in an insulin injection site in a diabetic patient. | 2014 | 24628302 | |
| clinical experience in 52 patients with tigecycline-containing regimens for salvage treatment of mycobacterium abscessus and mycobacterium chelonae infections. | we report the largest clinical experience using tigecycline-containing regimens for salvage treatment of patients with mycobacterium abscessus and mycobacterium chelonae. | 2014 | 24633206 |
| detection and assessment of clarithromycin inducible resistant strains among korean mycobacterium abscessus clinical strains: pcr methods. | mycobacterium abscessus group belongs to a group of rapidly growing mycobacteria (rgm) and, following mycobacterium avium complex, is the second most common pathogen responsible for lung disease caused by nontuberculous mycobacteria (ntm). clarithromycin is known to be the key drug in the treatment of m. abscessus group disease, but a high failure rate of treatment response is reported due to clarithromycin inducible resistance. | 2014 | 24652818 |
| lung transplantation in patients with cystic fibrosis: special focus to infection and comorbidities. | despite advances in medical care, patients with cystic fibrosis still face limited life expectancy. the most common cause of death remains respiratory failure. end-stage cystic fibrosis can be treated with lung transplantation and is the third most common reason for which the procedure is performed. outcomes for cystic fibrosis are better than most other lung diseases, but remain limited (5-year survival 60%). for patients with advanced disease lung transplantation appears to improve survival. o ... | 2014 | 24655065 |
| a cluster of endophthalmitis caused by mycobacterium abscessus after cataract surgery. | we report two cases of postoperative endophthalmitis after cataract surgery caused by the same strain of mycobacterium abscessus confirmed by arbitrarily primed polymerase chain reaction, sequencing of the erythromycin ribosome methyltransferase gene and pulsed-field gel electrophoresis. the outcomes were poor despite aggressive treatments. this is the first report of nontuberculous mycobacteria as a causative pathogen for a cluster of endophthalmitis. | 2016 | 24657068 |
| mycobacterium abscessus mab2560 induces maturation of dendritic cells via toll-like receptor 4 and drives th1 immune response. | in this study, we showed that mycobacterium abscessus mab2560 induces the maturation of dendritic cells (dcs), which are representative antigen-presenting cells (apcs). m. abscessus mab2560 stimulate the production of pro-inflammatory cytokines [interleukin (il)-6, tumor necrosis factor (tnf)-α, il-1β, and il-12p70] and reduce the endocytic capacity and maturation of dcs. using tlr4-/- dcs, we found that mab2560 mediated dc maturation via toll-like receptor 4 (tlr4). mab2560 also activated the m ... | 2014 | 24667171 |
| clonal relationship and differentiation among mycobacterium abscessus isolates as determined using the semiautomated repetitive extragenic palindromic sequence pcr-based diversilab system. | mycobacterium abscessus is a rapidly growing mycobacterium that causes respiratory tract infections in predisposed patients, such as those with cystic fibrosis and nosocomial skin and soft tissue infections. in order to investigate the clonal relationships between the strains causing epidemic episodes, we evaluated the discriminatory power of the semiautomated diversilab (dl) repetitive extragenic palindromic sequence pcr (rep-pcr) test for m. abscessus genotyping. since m. abscessus was shown t ... | 2014 | 24671795 |
| bacteraemia caused by mycobacterium abscessus subsp. abscessus and m. abscessus subsp. bolletii: clinical features and susceptibilities of the isolates. | mycobacterium abscessus complex (m. abscessus subsp. abscessus and m. abscessus subsp. bolletii) is an emerging pathogen causing various human infections. however, few studies have focused on m. abscessus complex bacteraemia with detailed species differentiation. the clinical characteristics of patients with bacteraemia due to m. abscessus complex treated at national taiwan university hospital from 2005-2012 were evaluated. species identification was performed by molecular methods, and minimum i ... | 2014 | 24718088 |
| mycobacterium tuberculosis pili (mtp), a putative biomarker for a tuberculosis diagnostic test. | novel biomarkers are urgently needed for point of care tb diagnostics. in this study, we investigated the potential of the pilin subunit protein encoded by the mtp gene as a diagnostic biomarker. blast analysis of the mtp gene on published genome databases, and amplicon sequencing were performed in mycobacterium tuberculosis complex (mtbc) strains and other organisms. the protein secondary structure of the amino acid sequences of non-tuberculous mycobacteria that partially aligned with the mtp s ... | 2014 | 24721207 |
| cefoxitin continuous infusion for lung infection caused by the mycobacterium abscessus group. | 2014 | 24733469 | |
| gene replacement in mycobacterium chelonae: application to the construction of porin knock-out mutants. | mycobacterium chelonae is a rapidly growing mycobacterial opportunistic pathogen closely related to mycobacterium abscessus that causes cornea, skin and soft tissue infections in humans. although m. chelonae and the emerging mycobacterial pathogen m. abscessus have long been considered to belong to the same species, these two microorganisms considerably differ in terms of optimum growth temperature, drug susceptibility, pathogenicity and the types of infection they cause. the whole genome sequen ... | 2014 | 24739882 |
| surgical treatment of nontuberculous mycobacterial lung disease. | while the prevalence of pulmonary tuberculosis has been decreasing, the prevalence of nontuberculous mycobacterial lung disease has been increasing. unlike tuberculosis, nontuberculous mycobacterial disease is not communicable. however, their indolent nature may result in extensive parenchymal destruction, causing respiratory failure and vulnerability to airway infection. nontuberculous mycobacterial lung disease, therefore, has been becoming a significant health problem. according to the 2007 a ... | 2014 | 24740640 |
| lack of antimicrobial bactericidal activity in mycobacterium abscessus. | antibiotic therapy of infections caused by the emerging pathogen mycobacterium abscessus is challenging due to the organism's natural resistance toward most clinically available antimicrobials. we investigated the bactericidal activity of antibiotics commonly administered in m. abscessus infections in order to better understand the poor therapeutic outcome. time-kill curves were generated for clinical m. abscessus isolates, mycobacterium smegmatis, and escherichia coli by using antibiotics commo ... | 2014 | 24752273 |
| mycobacterium abscessus hand-and-foot disease in children: rare or emerging disease? | mycobacterium abscessus is emerging as an important cause of cutaneous infections in sporadic cases and outbreak settings. although immunosuppressed or elderly patients are most commonly affected, in 2006 an outbreak of clinically distinct cutaneous lesions on the hands and feet caused by m. abscessus in a population of healthy children using a public swimming pool was reported. this article describes an outbreak of skin infection in a population of healthy italian children attending the same sc ... | 2014 | 24758202 |
| [case report; disseminated mycobacterium abscessus infection with hemophagocytic syndrome during treatment of chronic lymphocytic leukemia]. | 2014 | 24796145 | |
| in vivo assessment of drug efficacy against mycobacterium abscessus using the embryonic zebrafish test system. | mycobacterium abscessus is responsible for a wide spectrum of clinical syndromes and is one of the most intrinsically drug-resistant mycobacterial species. recent evaluation of the in vivo therapeutic efficacy of the few potentially active antibiotics against m. abscessus was essentially performed using immunocompromised mice. herein, we assessed the feasibility and sensitivity of fluorescence imaging for monitoring the in vivo activity of drugs against acute m. abscessus infection using zebrafi ... | 2014 | 24798271 |
| rapid identification of strains belonging to the mycobacterium abscessus group through erm(41) gene pyrosequencing. | mycobacterium abscessus and mycobacterium massiliense lung infections have different clarithromycin susceptibilities, making proper identification important; however, standard multi-gene sequencing in clinical laboratories is laborious and time consuming. we developed a pyrosequencing-based method for rapid identification of strains belonging to the m. abscessus group by targeting erm(41). we examined 55 isolates from new pulmonary m. abscessus infections and identified 28 m. abscessus, 25 m. ma ... | 2014 | 24809859 |
| highlights of the north american cf conference 2013. | this is a personal selection of papers that were presented at the 27th north american cystic fibrosis conference held in salt lake city in october 2013. the papers discussed in this review are thought to be of particular interest to cf caregivers in the uk. | 2014 | 24821547 |
| recurrent nontuberculous mycobacterial endophthalmitis: a diagnostic conundrum. | to report a case of recurrent nontuberculous mycobacterial endophthalmitis in the context of neurotrophic keratopathy secondary to herpes zoster ophthalmicus that had an atypical presentation and complex course, and highlights the challenges of causative organism identification and therapeutic interventions in this condition. | 2014 | 24833891 |