Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| food-safety hazards in the pork chain in nagaland, north east india: implications for human health. | pork occupies an important place in the diet of the population of nagaland, one of the north east indian states. we carried out a pilot study along the pork meat production chain, from live animal to end consumer. the goal was to obtain information about the presence of selected food borne hazards in pork in order to assess the risk deriving from these hazards to the health of the local consumers and make recommendations for improving food safety. a secondary objective was to evaluate the utilit ... | 2013 | 24368430 |
| identification of brucella suis from feral swine in selected states in the usa. | serologic tests currently available for brucellosis diagnosis detect antibodies to brucella but do not distinguish between species of brucella. although brucella suis is known to circulate within various feral swine (sus scrofa) populations, our objective was to determine the primary species of brucella circulating in feral swine populations in areas of the us with high brucellosis prevalence. we cultured lymph nodes from 183 feral swine. we identified 22 isolates from 21 animals, and all isolat ... | 2014 | 24484477 |
| the brucella suis ibpa heat-shock chaperone is not required for virulence or for expression of the virb type iv secretion system virb8 protein. | brucella suis, facultative intracellular bacterial pathogen of mammals, and agrobacterium tumefaciens, a plant pathogen, both use a virb type iv secretion system (t4ss) to translocate effector molecules into host cells. hspl, an α-crystalline-type small heat-shock protein, acts as a chaperone for the agrobacterium virb8 protein, an essential component of the virb system. an agrobacterium mutant lacking hspl is attenuated due to a misfunctional t4ss. we have investigated whether ibpa (bra0051), t ... | 2014 | 24517122 |
| comparative whole genome analysis of six diagnostic brucellaphages. | whole genome sequencing of six diagnostic brucellaphages, tbilisi (tb), firenze (fz), weybridge (wb), s708, berkeley (bk) and r/c, was followed with genomic comparisons including recently described genomes of the tb phage from mexico (tbm) and pr phage to elucidate genomic diversity and candidate host range determinants. comparative whole genome analysis revealed high sequence homogeneity among these brucellaphage genomes and resolved three genetic groups consistent with defined host range pheno ... | 2014 | 24530704 |
| brucella pinnipedialis hooded seal (cystophora cristata) strain in the mouse model with concurrent exposure to pcb 153. | brucellosis, a worldwide zoonosis, is linked to reproductive problems in primary hosts. a high proportion of brucella-positive hooded seals (cystophora cristata) have been detected in the declined northeast atlantic stock. high concentrations of polychlorinated biphenyls (pcbs) have also been discovered in top predators in the arctic, including the hooded seal, pcb 153 being most abundant. the aim of this study was to assess the pathogenicity of brucella pinnipedialis hooded seal strain in the m ... | 2014 | 24534631 |
| pathogen presence in feral pigs and their movement around two commercial piggeries in queensland, australia. | feral pigs are wild animal reservoirs of infectious pathogens transmissible to other species, all of which are transmissible to domestic pigs. the objective of this study was to detect the presence of harmful production-limiting pathogens; brucella suis, leptospira species, lawsonia intracellularis, mycoplasma hyopneumoniae and actinobacillus pleuropneumoniae in a feral pig population within a 10 km radius of two large-scale commercial piggeries in southern queensland, australia. the movement pa ... | 2014 | 24572722 |
| brucella suis in armadillos (chaetophractus villosus) from la pampa, argentina. | brucellosis is a zoonotic disease transmitted from an animal reservoir to humans. both, wildlife and domestic animals, contribute to the spreading of these zoonosis. the surveillance of the animal health status is strictly regulated for domestic animals, whereas disease monitoring in wildlife does not exist. the aim of the present study was to provide data on the prevalence of anti-brucella antibodies in chaetophractus villosus from a region of la pampa, argentina to assess public health risks. ... | 2014 | 24685240 |
| first report of a brucella suis infection in roe deer (capreolus capreolus). | in the present case report the detection of brucella (b.) suis biovar 2 in roe deer (capreolus capreolus) is described for the first time. the roe deer fawn was found emaciated and moribund in a hunting ground in the district hohenlohe in baden-württemberg, germany, in february 2013. a post-mortem examination revealed particularly a high-grade fibrinous pleurisy caused by the pathogen which could be multiplied in a dense growth on sheep blood agar and confirmed and differentiated subsequently by ... | 2014 | 24693656 |
| serological survey of brucella canis in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 24830899 |
| a repa-based elisa for discriminating cattle vaccinated with brucella suis 2 from those naturally infected with brucella abortus and brucella melitensis. | the commonest ways of diagnosing brucellosis in animals include the rose-bengal plate agglutination test, the buffered plate agglutination test (bpa), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (i-elisa). however, these methods cannot discriminate the brucella vaccine strain (brucella suis strain 2; b. suis s2) from naturally acquired virulent strains. of the six common brucella species, brucella melitensis, brucella abortus, an ... | 2014 | 24941369 |
| detection of brucellosis and leptospirosis in feral pigs in new south wales. | to determine the presence and estimate the prevalence of brucella suis, leptospira interrogans serovar pomona (hereafter l. pomona) and leptospira borgpetersenii serovar hardjo (hereafter l. hardjo) in feral pigs culled in new south wales (nsw), australia. | 2014 | 24954403 |
| genetic relatedness of brucella suis biovar 2 isolates from hares, wild boars and domestic pigs. | porcine brucellosis generally manifests as disorders in reproductive organs potentially leading to serious losses in the swine industry. brucella suis biovar 2 is endemic in european wild boar (sus scrofa) and hare (lepus europeus, lepus capensis) populations, thus these species may play a significant role in disease spread and serve as potential sources of infection for domestic pigs. the aim of this study was an epidemiologic analysis of porcine brucellosis in hungary and a comparative analysi ... | 2014 | 24962519 |
| advancement of knowledge of brucella over the past 50 years. | fifty years ago, bacteria in the genus brucella were known to cause infertility and reproductive losses. at that time, the genus was considered to contain only 3 species: brucella abortus, brucella melitensis, and brucella suis. since the early 1960s, at least 7 new species have been identified as belonging to the brucella genus (brucella canis, brucella ceti, brucella inopinata, brucella microti, brucella neotomae, brucella ovis, and brucella pinnipedialis) with several additional new species u ... | 2014 | 24981716 |
| complete genome sequences of three iberian brucella suis biovar 2 strains isolated from wild boars. | brucella suis biovar 2 is the most common biovar isolated from wild boars (sus scrofa) associated with transmission to outdoor-reared pigs in europe. we report here the complete and annotated genome sequences of three strains isolated from wild boars in portugal and spain and belonging to the iberian clone (haplotypes 2d and 2e). | 2014 | 24994794 |
| duplex pcr for differentiation of the vaccine strain brucella suis s2 and b. suis biovar 1 from other strains of brucella spp. | immunisation with attenuated brucella spp. vaccines prevents brucellosis, but may also interfere with diagnosis. in this study, a duplex pcr was developed to distinguish brucella suis vaccine strain s2 from field strains of b. suis biovar 1 and other brucella spp. the pcr detected 60 fg genomic dna of b. suis s2 or biovar 1 field strains and was able to distinguish b. suis s2 and wild-type strains of b. suis biovar 1 among 76 field isolates representing all the common species and biovars, as wel ... | 2014 | 25011712 |
| complete genome sequences of two central european brucella suis bv. 2 haplotype 2c strains isolated from wild boars. | the brucella suis haplotype 2c is commonly isolated from wild boars and domestic pigs across central europe, though it is rarely described in the iberian peninsula. we report here the complete and annotated genome sequences of two haplotype 2c strains isolated from wild boars in the northeast region of spain, above the ebro river. | 2014 | 25013144 |
| galleria mellonella as an infection model for select agents. | the use of animal models is a key step to better understand bacterial virulence factors and their roles in host/pathogen interactions. to avoid the ethical and cost problems of mammalian models in bacterial virulence research, several insect models have been developed. one of these models, the larvae of the greater wax moth galleria mellonella, has been shown to be relevant for several fungal and bacterial mammalian pathogens. here, we describe the use g. mellonella to study virulence of the hig ... | 2014 | 25172272 |
| oxo- and thiooxo-imidazo[1,5-c]pyrimidine molecule library: beyond their interest in inhibition of brucella suis histidinol dehydrogenase, a powerful protection tool in the synthesis of histidine analogues. | histidinol dehydrogenase (hdh) has been established as a virulence factor for the human pathogen bacterium brucella suis. targeting such a virulence factor is a relevant anti-infectious approach as it could decrease the frequency of antibiotic resistance appearance. in this paper, we describe the synthesis of a family of oxo- and thioxo-imidazo[1,5-c]pyrimidines, potential enzyme inhibitors. beyond their anti-hdh activity, the synthesis approach of these molecules, never described before, is hig ... | 2014 | 25278235 |
| draft genome sequences of brucella suis biovar 4 strain nctc 10385, brucella ceti strain nctc 12891t, brucella inopinata strain camp 6436t, and brucella neotomae strain atcc 23459t. | with the aim of developing quantitative pcr methods for the detection and differentiation of brucella species, the genomes of brucella ceti, brucella inopinata, brucella netotomae, and brucella suis biovar 4 were sequenced and analyzed. | 2014 | 25278518 |
| c-glycosides incorporating the 6-methoxy-2-naphthyl moiety are selective inhibitors of fungal and bacterial carbonic anhydrases. | a small series of c-glycosides containing the methoxyaryl moieties was tested for the inhibition of the β-class carbonic anhydrases (cas, ec 4.2.1.1) from cryptococcus neoformans and brucella suis. many compounds showed activities in the micromolar or submicromolar range and excellent selectivity for pathogen cas over human isozymes. the deprotected glycosides incorporating the 6-methoxy-2-naphthyl moiety showed the best inhibition profile and therefore represent leads for the development of nov ... | 2015 | 25291009 |
| thermostable cross-protective subunit vaccine against brucella species. | a subunit vaccine candidate was produced from brucella suis 145 (biovar 4; expressing both the a antigen of brucella abortus and the m antigen of brucella melitensis). the preparation consisted mostly of polysaccharide (ps; >90% [wt/wt]; both cell-associated ps and exo-ps were combined) and a small amount of protein (1 to 3%) with no apparent nucleic acids. vaccinated mice were protected (these had a statistically significant reduction in bacterial colonization compared to that of unvaccinated c ... | 2014 | 25320267 |
| genome sequences of brucella abortus and brucella suis strains isolated from bovine in zimbabwe. | this is a report of whole-genome sequences of a brucella abortus strain and two brucella suis strains isolated from bovine in zimbabwe. these strains were selected based on their origin and data obtained when using multiplex pcr assays, then sequenced using next-generation sequencing technologies. | 2014 | 25342680 |
| studies on a suitable antibiotic therapy for treating swine brucellosis. | the aim of this work was developing effective treatments against brucella suis biovar 2, responsible for swine brucellosis in europe. mics for antibiotics used classically in brucellosis and two new macrolides (tulathromycin and tildipirosin) were determined for 33 b. suis biovar 2 field and b. suis reference strains. mic90 values ranged from 0.01 to 0.25 μg/ml. the best candidates, given alone or combined, were then evaluated in mice. ten groups (n = 7) of balb/c mice were inoculated (1 × 10(5) ... | 2015 | 25413993 |
| in vitro bactericidal activity of aminoglycosides, including the next-generation drug plazomicin, against brucella spp. | plazomicin is a next-generation aminoglycoside with a potentially unique set of clinical characteristics compared with other aminoglycosides. this study assessed the minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs) of plazomicin against 15 clinical isolates as well as three reference strains representing brucella abortus, brucella melitensis and brucella suis. these data were compared with those obtained for six other aminoglycosides and two aminocyclitols. ... | 2015 | 25459738 |
| clinical and histological features of brucellin skin test responses in brucella suis biovar 2 infected pigs. | current serological tests for swine brucellosis detect antibodies to the brucella o-polysaccharide (o/ps). however, when infections by bacteria carrying cross-reacting o/ps occur, these tests suffer from false positive serological reactions (fpsr), and the skin test with brucella soluble protein extracts is the best diagnostic alternative to differentiate true brucella suis infections from fpsr in pigs. since this test has been seldom used in b. suis infected swine, the clinical and histological ... | 2015 | 25480681 |
| brucella suis infection in domestic pigs in sardinia (italy). | during a 4-year (2007-2010) survey, the presence of brucella suis infection in domestic pigs in sardinia was investigated. serum samples were collected from breeding pigs located on 108 commercial farms with documented reproductive problems and analysed using the rose bengal (rbt) and complement fixation (cft) tests for screening and confirmation of brucella, respectively. of the 1251 serum samples analysed by rbt, 406 sera, originating from 36 farms, were positive for b. suis. cft was positive ... | 2015 | 25530227 |
| molecular cloning, expression and characterization of programmed cell death 10 from sheep (ovis aries). | programmed cell death 10 (pdcd10) is a highly conserved adaptor protein. its mutations result in cerebral cavernous malformations (ccms). in this study, pdcd10 cdna from the buffy coat of small tail han sheep (ovis aries) was cloned from a suppression subtractive hybridization cdna library, named oapdcd10. the full-length cdna of oapdcd10 was 1343bp with a 639bp open reading frame (orf) encoding 212 amino acid residues. tissue distribution of oapdcd10 mrna determined that it was ubiquitously exp ... | 2015 | 25541025 |
| brucella suis vaccine strain s2-infected immortalized caprine endometrial epithelial cell lines induce non-apoptotic er-stress. | brucella, which is regarded as an intracellular pathogen responsible for a zoonotic disease called brucellosis, survives and proliferates within several types of phagocytic and non-phagocytic cells. brucella infects not only their preferred hosts but also other domestic and wild animal species, inducing abortion and infertility. therefore, the interaction between uterine cells and brucella is important for understanding the pathogenesis of this disease. in this study, we describe the brucella su ... | 2015 | 25633898 |
| diagnostic performance of serological tests for swine brucellosis in the presence of false positive serological reactions. | swine brucellosis caused by brucella suis biovar 2 is an emerging disease in europe. currently used diagnostic tests for swine brucellosis detect antibodies to the o-polysaccharide (o-ps) of brucella smooth lipopolysaccharide (s-lps) but their specificity is compromised by false-positive serological reactions (fpsrs) when bacteria carrying cross-reacting o-ps infect pigs. fpsrs occur throughout europe, and the only tool available for a specific b. suis diagnosis is the intradermal test with bruc ... | 2015 | 25661496 |
| inhibition of β-carbonic anhydrases from brucella suis with c-cinnamoyl glycosides incorporating the phenol moiety. | a small series of c-glycosides containing the phenol moiety was tested for the inhibition of the β-class carbonic anhydrases (βcas, ec 4.2.1.1) from brucella suis. many compounds showed activities in the micromolar or submicromolar range and excellent selectivity for pathogen cas over human isozymes. glycosides incorporating the 3-hydroxyphenyl moiety showed the best inhibition profile, and therefore this functionality represents lead for the development of novel anti-infectives with a new mecha ... | 2015 | 25676329 |
| n-glycosyl-n-hydroxysulfamides as potent inhibitors of brucella suis carbonic anhydrases. | we investigated a series of n-hydroxysulfamides obtained by ferrier sulfamidoglycosylation for the inhibition of two bacterial carbonic anhydrases (cas, ec 4.2.1.1) present in the pathogen brucella suis. bsca i was moderately inhibited by these compounds with inhibition constants ranging between 522 and 958 nm and no notable differences of activity between the acetylated or the corresponding deacetylated derivatives. the compounds incorporating two trans-acetates and the corresponding deprotecte ... | 2015 | 25792504 |
| comparison of brucella canis genomes isolated from different countries shows multiple variable regions. | brucella canis is a pathogenic bacterium for dogs and its zoonotic potential has been increasing in recent years. in this study, we report the sequencing, annotation and analysis of the genome of brucella canis strain oliveri isolated from a dog in a breeding kennel in medellín, colombia, south america. whole genome shotgun sequencing was carried out using the roche 454 gs flx titanium technology at the national center for genomic sequencing-cnsg in medellin, colombia. the assembly procedure was ... | 2015 | 25820207 |
| structural basis for the inhibition of helicobacter pylori α-carbonic anhydrase by sulfonamides. | periplasmic α-carbonic anhydrase of helicobacter pylori (hpαca), an oncogenic bacterium in the human stomach, is essential for its acclimation to low ph. it catalyses the conversion of carbon dioxide to bicarbonate using zn(ii) as the cofactor. in h. pylori, neisseria spp., brucella suis and streptococcus pneumoniae this enzyme is the target for sulfonamide antibacterial agents. we present structural analysis correlated with inhibition data, on the complexes of hpαca with two pharmacological inh ... | 2015 | 26010545 |
| immune responses and protection induced by brucella suis s2 bacterial ghosts in mice. | with the purpose of generating brucella suis bacterial ghosts and investigating the immunogenicity of bacterial ghosts as a vaccine candidate, the lysis gene e and temperature-sensitive regulator cassette were cloned into a shuttle plasmid, pbbr1mcs-2, for construction of a recombinant temperature-sensitive shuttle lysis plasmid, pbbr1mcs-e. pbbr1mcs-e was then introduced into attenuated b. suis live vaccine s2 bacteria, and the resultant transformants were used for production of b. suis ghosts ... | 2015 | 26022514 |
| multiple-locus variable-number tandem-repeat analysis (mlva) genotyping of human brucella isolates in malaysia. | brucellosis is one of the most common zoonotic diseases worldwide. it can cause acute febrile illness in human and is a major health problem. studies in human brucellosis in malaysia is limited and so far no genotyping studies has been done on brucella isolates. the aim of the study was to determine the genetic diversity among brucella species isolated from human brucellosis, obtained over a 6-year period (2009-2014). | 2015 | 26033227 |
| first report of brucella suis biovar 2 in a semi free-range pig farm, italy. | this communication describes the isolation of brucella suis (b. suis) biovar 2 in semi‑free‑range pigs located in the province of rome, italy. sera of 28 pigs from a herd with reproductive problems were tested for brucellosis. twenty-five sera (89%) were found positive to rose bengal test (rbt), while 22 (79%) were positive to complement fixation test (cft). two positive pigs were slaughtered, organs were collected and tested for the presence of bacteria. brucella spp. was isolated from the sple ... | 2015 | 26129667 |
| inhibition studies of bacterial, fungal and protozoan β-class carbonic anhydrases with schiff bases incorporating sulfonamide moieties. | a series of new schiff bases derived from sulfanilamide, 3-fluorosulfanilamide or 4-(2-aminoethyl)-benzenesulfonamide containing either a hydrophobic or a hydrophilic tail, have been investigated as inhibitors of three β-carbonic anhydrases (ca, ec 4.2.1.1) from three different microorganisms. their antifungal, antibacterial and antiprotozoan activities have been determined against the pathogenic fungus cryptococcus neoformans, the bacterial pathogen brucella suis and the protozoan parasite leis ... | 2015 | 26145821 |
| [the fragments of dead macrophages infected by brucellasuis s2 strain activates the immune response against brucella infection]. | to investigate the role of the dead macrophages infected by brucella suis s2 strain in the initiation of immune response to brucella. | 2015 | 26146061 |
| bacterial, fungal and protozoan carbonic anhydrases as drug targets. | the carbonic anhydrases (cas, ec 4.2.1.1), a group of ubiquitously expressed metalloenzymes, are involved in numerous physiological and pathological processes, as well as in the growth and virulence of pathogens belonging to bacteria, fungi and protozoa. | 2015 | 26235676 |
| a serosurvey for brucellosis in wild boar (sus scrofa) in sardinia, italy. | porcine brucellosis is a zoonotic disease caused by brucella suis and hosted by pigs (sus scrofa). both domestic pigs and wild boars are affected. we measured the prevalence of antibody to brucella spp. in wild boars in sardinia, italy. during 1 november 2009 to 31 january 2010, we collected 570 serum samples from legally hunted wild boars and tested them using a commercial competitive enzyme-linked immunosorbent assay. sex and age class of the sampled wild boars were also recorded. thirty-five ... | 2015 | 26267463 |
| [identification of zoonotic bacterial pathogens by the maldi tof ms method]. | to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work. | 2015 | 26312374 |
| characterisation of brucella suis isolates from southeast europe by multi-locus variable-number tandem repeat analysis. | porcine brucellosis is a common bacterial zoonosis which can cause significant financial losses. its diverse and often complicated factors have hampered efforts to control disease spread. the aim of the study was to assess the epidemiological situation of porcine brucellosis primarily in croatia and its relationship to genotypes present in other, mostly european countries. one hundred and seven brucella suis strains isolated from swine, hares, cattle, humans, wild hares, a wild boar and a mare o ... | 2015 | 26324171 |
| novel real-time pcr detection assay for brucella suis. | brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in europe. both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. available serological or pcr based methods lack sensitivity and specificity. | 2015 | 26392898 |
| a novel lumazine synthase molecule from brucella significantly promotes the immune-stimulation effects of antigenic protein. | brucella, an intracellular parasite that infects some livestock and humans, can damage or destroy the reproductive system of livestock. the syndrome is referred to as brucellosis and often occurs in pastoral areas; it is contagious from livestock to humans. in this study, the intact brucella suis outer membrane protein 31 (omp31) gene was cloned, recombinantly expressed, and examined as a subunit vaccine candidate. the intact brucella lumazine synthase (bls) gene was cloned and recombinantly exp ... | 2015 | 26535621 |
| anti-brucella antibodies in moose (alces alces gigas), muskoxen (ovibos moschatus), and plains bison (bison bison bison) in alaska, usa. | we used an indirect enzyme-linked immunosorbent assay (ielisa) and the rose bengal test (rbt) to test for anti-brucella antibodies in moose (alces alces gigas), muskoxen (ovibos moschatus), and plains bison (bison bison bison) from various game management units (gmus) in alaska, us, sampled from 1982 to 2010. a portion of the sera had previously been tested with the standard plate test (spt), the buffered brucella antigen (bba) card test, and the card test (card). no antibody-positive plains bis ... | 2016 | 26540335 |
| transcriptome-wide identification of hfq-associated rnas in brucella suis by deep sequencing. | recent breakthroughs in next-generation sequencing technologies have led to the identification of small noncoding rnas (srnas) as a new important class of regulatory molecules. in prokaryotes, srnas are often bound to the chaperone protein hfq, which allows them to interact with their partner mrna(s). we screened the genome of the zoonotic and human pathogen brucella suis 1330 for the presence of this class of rnas. we designed a coimmunoprecipitation strategy that relies on the use of hfq as a ... | 2016 | 26553849 |
| brucella suis strain 2 vaccine is safe and protective against heterologous brucella spp. infections. | brucellosis is a wide spread zoonotic disease that causes abortion and infertility in mammals and leads to debilitating, febrile illness in humans. brucella abortus, brucella melitensis and brucella suis are the major pathogenic species to humans. vaccination with live attenuated b. suis strain 2 (s2) vaccine is an essential and critical component in the control of brucellosis in china. the s2 vaccine is very effective in preventing brucellosis in goats, sheep, cattle and swine. however, there a ... | 2016 | 26626213 |
| identification of source of brucella suis infection in human by using whole-genome sequencing, united states and tonga. | brucella suis infection was diagnosed in a man from tonga, polynesia, who had butchered swine in oregon, usa. although the us commercial swine herd is designated brucellosis-free, exposure history suggested infection from commercial pigs. we used whole-genome sequencing to determine that the man was infected in tonga, averting a field investigation. | 2016 | 26689610 |
| evaluating the risk of pathogen transmission from wild animals to domestic pigs in australia. | wild animals contribute to endemic infection in livestock as well as the introduction, reintroduction and maintenance of pathogens. the source of introduction of endemic diseases to a piggery is often unknown and the extent of wildlife contribution to such local spread is largely unexplored. the aim of the current study was to quantitatively assess the probability of domestic pigs being exposed to different pathogens from wild animals commonly found around commercial piggeries in australia. spec ... | 2016 | 26711303 |
| the complete genome of brucella suis 019 provides insights on cross-species infection. | brucella species are the most important zoonotic pathogens worldwide and cause considerable harm to humans and animals. in this study, we presented the complete genome of b. suis 019 isolated from sheep (ovine) with epididymitis. b. suis 019 has a rough phenotype and can infect sheep, rhesus monkeys and possibly humans. the comparative genome analysis demonstrated that b. suis 019 is closest to the vaccine strain b. suis bv. 1 str. s2. further analysis associated the rsh gene to the pathogenicit ... | 2016 | 26821047 |
| bioluminescence imaging of colonization and clearance dynamics of brucella suis vaccine strain s2 in mice and guinea pigs. | the goal of this study was to develop a plasmid-based lux bio-reporter for use to obtain in vivo images of brucella suis vaccine strain 2 (b.suis s2) infection with high resolution and good definition. | 2016 | 26846129 |
| delta-pgm, a new live-attenuated vaccine against brucella suis. | brucellosis is one of the most widespread zoonosis in the world affecting many domestic and wild animals including bovines, goats, pigs and dogs. each species of the brucella genus has a particular tropism toward different mammals being the most relevant for human health brucella abortus, brucella melitensis and brucella suis that infect bovines, goats/camelids and swine respectively. although for b. abortus and b. melitensis there are vaccines available, there is no efficient vaccine to protect ... | 2016 | 26899373 |
| efficacy of antibiotic treatment and test-based culling strategies for eradicating brucellosis in commercial swine herds. | swine brucellosis caused by brucella suis biovar 2 is an emerging disease in continental europe. without effective vaccines being available, the european food safety authority (efsa) recommends the full depopulation of infected herds as the only strategy to eradicate b. suis outbreaks. using data collected from 8 herds suffering natural swine brucellosis outbreaks, we assessed the efficacy of four control strategies: (i) oxytetracycline treatment only, as a default scenario, (ii) oxytetracycline ... | 2016 | 26899897 |
| brucella suis vaccine strain 2 induces endoplasmic reticulum stress that affects intracellular replication in goat trophoblast cells in vitro. | brucella has been reported to impair placental trophoblasts, a cellular target where brucella efficiently replicates in association with the endoplasmic reticulum (er), and ultimately trigger abortion in pregnant animals. however, the precise effects of brucella on trophoblast cells remain unclear. here, we describe the infection and replication of brucella suis vaccine strain 2 (b.suis.s2) in goat trophoblast cells (gtcs) and the cellular and molecular responses induced in vitro. our studies de ... | 2016 | 26904517 |
| a case-study of implementation of improved strategies for prevention of laboratory-acquired brucellosis. | in 2012, the alaska section of epidemiology investigated personnel potentially exposed to a brucella suis isolate as it transited through three laboratories. | 2015 | 26929849 |
| the first report of brucella suis biovar 1 isolation in human in turkey. | brucella melitensis and b. abortus are the species generally isolated from human samples in turkey. several studies have also demonstrated the presence of antibodies against b. canis. | 2017 | 26947547 |
| marine mammal brucella reference strains are attenuated in a balb/c mouse model. | brucellosis is a zoonosis of worldwide distribution with numerous animal host species. since the novel isolation of brucella spp. from marine mammals in 1994 the bacteria have been isolated from various marine mammal hosts. the marine mammal reference strains brucella pinnipedialis 12890 (harbour seal, phoca vitulina) and brucella ceti 12891 (harbour porpoise, phocoena phocoena) were included in genus brucella in 2007, however, their pathogenicity in the mouse model is pending. herein this is ev ... | 2016 | 26959235 |
| a bacterial glycoengineered antigen for improved serodiagnosis of porcine brucellosis. | brucellosis is a highly zoonotic disease that affects animals and human beings. brucella suis is the etiological agent of porcine brucellosis and one of the major human brucellosis pathogens. laboratory diagnosis of porcine brucellosis mainly relies on serological tests, and it has been widely demonstrated that serological assays based on the detection of anti o-polysaccharide antibodies are the most sensitive tests. here, we validate a recombinant glycoprotein antigen, an n-formylperosamine o-p ... | 2016 | 26984975 |
| immunogenicity analysis of a novel subunit vaccine candidate molecule-recombinant l7/l12 ribosomal protein of brucella suis. | brucella was an intracellular parasite, which could infect special livestock and humans. after infected by brucella, livestock's reproductive system could be affected and destroyed resulting in huge economic losses. more seriously, it could be contagious from livestock to humans. so far, there is no available vaccine which is safe enough for humans. on this point, subunit vaccine has become the new breakthrough of conquering brucellosis. in this study, brucella rl7/l12-bls fusion protein was use ... | 2016 | 27075455 |
| a genome-wide snp-based phylogenetic analysis distinguishes different biovars of brucella suis. | brucellosis is an important zoonotic disease caused by brucella spp. brucella suis is the etiological agent of porcine brucellosis. b. suis is the most genetically diverged species within the genus brucella. we present the first large-scale b. suis phylogenetic analysis based on an alignment-free k-mer approach of gathering polymorphic sites from whole genome sequences. genome-wide core-snp based phylogenetic tree clearly differentiated and discriminated the b. suis biovars and the vaccine strai ... | 2016 | 27085292 |
| brucella suis bacteremia misidentified as ochrobactrum anthropi by the vitek 2 system. | ochrobactrum and brucella are genetically related genera of the family brucellaceae, sharing 98.8% rrna similarity. because of their phenotypic similarity, ochrobactrum can be miscoded as brucella by automated identification systems. the misidentification on blood cultures (bcs) of b. suis as o. anthropi by the vitek 2 system is herein described. a 67-year-old male with a prosthetic mitral valve and fever was admitted with bacteremia due to a gram-negative coccobacillus identified as o. anthropi ... | 2016 | 27131010 |
| serological and molecular investigation for brucellosis in swine in selected districts of uganda. | brucellosis is a notifiable zoonotic disease affecting livestock, humans, and wildlife in uganda. pigs can be infected with human pathogenic brucella suis biovars 1 and 3 and can be a significant source of brucellosis for humans. uganda has a rapidly growing pig population, and the pork consumption per capita is the highest in east africa. the objective of this work was to determine the seroprevalence of brucellosis in ugandan pigs. a cross-sectional serosurvey of pigs was conducted in three of ... | 2016 | 27142028 |
| omics of brucella: species-specific srna-mediated gene ontology regulatory networks identified by computational biology. | brucella is an intracellular bacterium that causes the zoonotic infectious disease, brucellosis. brucella species are currently intensively studied with a view to developing novel global health diagnostics and therapeutics. in this context, small rnas (srnas) are one of the emerging topical areas; they play significant roles in regulating gene expression and cellular processes in bacteria. in the present study, we forecast srnas in three brucella species that infect humans, namely brucella melit ... | 2016 | 27223678 |
| contagious ecthyma, rangiferine brucellosis, and lungworm infection in a muskox ( ovibos moschatus ) from the canadian arctic, 2014. | an adult male muskox ( ovibos moschatus ), harvested on 26 august 2014 on victoria island, nunavut, in the canadian arctic, had proliferative dermatitis on the muzzle and fetlocks suggestive of contagious ecthyma or orf (parapoxvirus). histopathologic features of the lesions were consistent with this diagnosis. orf virus dna, phylogenetically similar to an isolate from a captive muskox of the minnesota zoo, us, was detected in the lesions by pcr using parapoxvirus primers. additionally, there wa ... | 2016 | 27285415 |
| activation of bovine neutrophils by brucella spp. | brucellosis is a globally important zoonotic infectious disease caused by gram negative bacteria of the genus brucella. while many species of brucella exist, brucella melitensis, brucella abortus, and brucella suis are the most common pathogens of humans and livestock. the virulence of brucella is largely influenced by its ability to evade host factors, including phagocytic killing mechanisms, which are critical for the host response to infection. the aim of this study was to characterize the bo ... | 2016 | 27436438 |
| epidemiological investigation of the first human brucellosis case in spain due to brucella suis biovar 1 strain 1330. | no cases of human brucellosis caused by brucella suis has been reported in spain. | 2017 | 27474211 |
| whole-genome mapping reveals a large chromosomal inversion on iberian brucella suis biovar 2 strains. | optical mapping is a technology able to quickly generate high resolution ordered whole-genome restriction maps of bacteria, being a proven approach to search for diversity among bacterial isolates. in this work, optical whole-genome maps were used to compare closely-related brucella suis biovar 2 strains. this biovar is the unique isolated in domestic pigs and wild boars in portugal and spain and most of the strains share specific molecular characteristics establishing an iberian clonal lineage ... | 2016 | 27527786 |
| emergence of brucella suis in dogs in new south wales, australia: clinical findings and implications for zoonotic transmission. | animal reservoirs of brucellosis constitute an ongoing threat to human health globally, with foodborne, occupational and recreational exposures creating opportunities for transmission. in australia and the united states, hunting of feral pigs has been identified as the principal risk factor for human brucellosis due to brucella suis. following increased reports of canine b. suis infection, we undertook a review of case notification data and veterinary records to address knowledge gaps about tran ... | 2016 | 27613248 |
| structural analysis and inhibition of trae from the pkm101 type iv secretion system. | gram-negative bacteria use type iv secretion systems (t4sss) for a variety of macromolecular transport processes that include the exchange of genetic material. the pkm101 plasmid encodes a t4ss similar to the well-studied model systems from agrobacterium tumefaciens and brucella suis here, we studied the structure and function of trae, a homolog of virb8 that is an essential component of all t4sss. analysis by x-ray crystallography revealed a structure that is similar to other virb8 homologs but ... | 2016 | 27634044 |
| comparative genomic analysis between newly sequenced brucella suis vaccine strain s2 and the virulent brucella suis strain 1330. | brucellosis is a bacterial disease caused by brucella infection. in the late fifties, brucella suis vaccine strain s2 with reduced virulence was obtained by serial transfer of a virulent b. suis biovar 1 strain in china. it has been widely used for vaccination in china since 1971. until now, the mechanisms underlie virulence attenuation of s2 are still unknown. | 2016 | 27645563 |
| high incidence of respiratory involvement in a cluster of brucella suis-infected workers from a pork processing plant in argentina. | epidemiological and clinical aspects of brucella suis infection in 17 workers from a pork processing plant in argentina occurring between january 2014 and july 2015 are presented. all patients reported working 9 h daily without adequate personal protection garment. blood cultures were positive for brucella spp. in 14 of the 17 patients (82.3%). all isolates were identified as b. suis biovar 1. although fever, sweats, asthenia, myalgia and hepatic involvement were the most frequent clinical manif ... | 2016 | 28032696 |
| extended multilocus sequence analysis to describe the global population structure of the genus brucella: phylogeography and relationship to biovars. | an extended multilocus sequence analysis (mlsa) scheme applicable to the brucella, an expanding genus that includes zoonotic pathogens that severely impact animal and human health across large parts of the globe, was developed. the scheme, which extends a previously described nine locus scheme by examining sequences at 21 independent genetic loci in order to increase discriminatory power, was applied to a globally and temporally diverse collection of over 500 isolates representing all 12 known b ... | 2016 | 28066370 |
| in this issue - january/february 2017: experimental hendra virus infection of dogs · brucella suis in dogs · cytokines in stored packed red blood cells · transitional lumbosacral vertebrae in labradors · chemotherapy for histiocytic sarcomas · pyothorax and oesophageal foreign bodies · osteochondroma in a horse · detection of bovine ephemeral fever virus. | 2017 | 28124421 | |
| clinical management of brucella suis infection in dogs and implications for public health. | brucellosis caused by brucella suis is a notifiable disease that has recently emerged in dogs in new south wales (nsw). given the potential for zoonotic transmission, euthanasia of affected dogs is recommended, but this action is not mandatory. we report the clinical management of three dogs that underwent treatment at their owners' request. | 2017 | 28124423 |
| serological survey of <i>brucella canis</i> in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 28235300 |
| monomer-to-dimer transition of brucella suis type iv secretion system component virb8 induces conformational changes. | secretion systems are protein complexes essential for bacterial virulence and potential targets for antivirulence drugs. in the intracellular pathogen brucella suis, a type iv secretion system mediates the translocation of virulence factors into host cells and it is essential for pathogenicity. virb8 is a core component of the secretion system and dimerization is important for functionality of the protein complex. we set out to study dimerization and possible conformational changes of virb8 from ... | 2017 | 28236662 |
| genome sequences of two brucella suis strains isolated from the same patient, 8 years apart. | brucella suis is a gram-negative, facultative intracellular pathogen that has pigs as its preferred host, but it can also infect humans. here, we report the draft genome sequences of two b. suis strains that were isolated from the same patient, 8 years apart. | 2017 | 28254974 |
| brucella suis carbonic anhydrases and their inhibitors: towards alternative antibiotics? | carbonic anhydrases have started to emerge as new potential antibacterial targets for several pathogens. two β-carbonic anhydrases, denominated bsca i and bsca ii, have been isolated and characterized from the bacterial pathogen brucella suis, the causative agent of brucellosis or malta fever. these enzymes have been investigated in detail and a wide range of classical aromatic and heteroaromatic sulfonamides as well as carbohydrate-based compounds have been found to inhibit selectively and effi ... | 2017 | 28274160 |
| boar hunting and brucellosis caused by brucella suis. | brucellosis remains as a significant public health concern in many areas where the infection persists in domestic hosts (i.e. goats, cattle, and domestic swine) with subsequent risk of transmission to human populations. brucellosis caused by b. suis remains an important threat to human populations in many countries exposed to domestic and feral swine. in the u.s., swine brucellosis has been under control for many years. meanwhile, it is a widespread infection among feral swine, particularly in t ... | 2017 | 28300645 |
| [serological detection of brucella suis, influenza virus and aujeszky's disease virus in backyard and small swine holders in argentina]. | farmers raising less than 100 sows represent more than 99% of swine producers in argentina, although little is known about their sanitary status and productive characteristics in the country. sanitary and productive information was obtained. furthermore, samples for serological studies were taken to detect antibodies against brucella suis (bs), aujeszky's disease virus (av) and influenza virus (iv) in 68 backyard and small producers with less than 100 sows located in the north, central and south ... | 2017 | 28325625 |
| rega plays a key role in oxygen-dependent establishment of persistence and in isocitrate lyase activity, a critical determinant of in vivo brucella suis pathogenicity. | for aerobic human pathogens, adaptation to hypoxia is a critical factor for the establishment of persistent infections, as oxygen availability is low inside the host. the two-component system regb/a of brucella suis plays a central role in the control of respiratory systems adapted to oxygen deficiency, and in persistence in vivo. using an original "in vitro model of persistence" consisting in gradual oxygen depletion, we compared transcriptomes and proteomes of wild-type and δrega strains to id ... | 2017 | 28573107 |
| brucella omp25 upregulates mir-155, mir-21-5p, and mir-23b to inhibit interleukin-12 production via modulation of programmed death-1 signaling in human monocyte/macrophages. | brucella spp. infection results in compromised type1 (th1) cellular immune response. several reports have described an immunomodulatory function for brucella major outer membrane protein omp25. however, the mechanism by which omp25 modulates macrophage dysfunction has not been defined. herein, we reported that omp25-deficient mutant of brucella suis exhibited an enhanced ability to induce interleukin (il)-12 whereas ectopic expression of omp25 protein inhibited tlr agonists-induced il-12 p70 pro ... | 2017 | 28694807 |
| brucella suis biovar 2 infection in humans in france: emerging infection or better recognition? | brucellosis is usually acquired by humans through contact with infected animals or the consumption of raw milk from infected ruminants. brucella suis biovar 2 (bsb2) is mainly encountered in hares and wild boars (sus scrofa), and is known to have very low pathogenicity to humans with only two case reports published in the literature. human cases of brucellosis caused by bsb2 were identified through the national mandatory notification of brucellosis. the identification of the bacterium species an ... | 2017 | 28784192 |
| vector-borne and zoonotic diseases of dogs in north-west new south wales and the northern territory, australia. | vector-borne diseases of dogs in australian aboriginal communities are relatively unexplored. these dogs represent a unique group with variable ecto- and endo-parasitic burdens, nutritional stresses and a general lack of veterinary intervention. we investigated haemoprotozoal and bacterial pathogen prevalences in relation to erythrocyte and platelet numbers in dogs from north-west new south wales (n-w nsw) and the northern territory (nt; central australia). | 2017 | 28810870 |
| trypanosoma infection favors brucella elimination via il-12/ifnγ-dependent pathways. | this study develops an original co-infection model in mice using brucella melitensis, the most frequent cause of human brucellosis, and trypanosoma brucei, the agent of african trypanosomiasis. although the immunosuppressive effects of t. brucei in natural hosts and mice models are well established, we observed that the injection of t. brucei in mice chronically infected with b. melitensis induces a drastic reduction in the number of b. melitensis in the spleen, the main reservoir of the infecti ... | 2017 | 28824630 |