Publications

serum amyloid a in marine bivalves: an acute phase and innate immunity protein.	serum amyloid a (saa) is among the most potent acute phase proteins (app) in vertebrates. after injury, its early expression can dramatically increase to promote the recruitment of immuno-competent cells, expression of pro-inflammatory proteins and the activation of the innate immune defences. although app have been studied in many vertebrates, only recently their search was extended to invertebrates and the finding of saa-like molecules has opened new questions on the immune-regulatory function ...	2016	26828389
levels of pcbs in oysters coming from galicia coast: comparison to mussels from the same region.	pcbs were analyzed in two species of oyster (crassostrea gigas and ostrea edulis) cultured in intertidal beds and rafts coming from the galician rías during the period 2011-2014. pcbs were also analyzed in mussel (mytilus galloprovincialis) collected in the same rías during 2011. the main objective of this work is to investigate the distribution of pcbs in galician oysters and to study their suitability as bioindicator in comparison to mussels. the levels of σpcbs (ten congeners) ranged from 5.5 ...	2016	26988224
effects of emerging contaminants on neurotransmission and biotransformation in marine organisms - an in vitro approach.	the effects of gold (ionic form and nanoparticles - aunps) and pharmaceuticals (carbamazepine and fluoxetine) on enzymes involved in neurotransmission (acetylcholinesterase - ache) and biotransformation (glutathione s-transferases - gst) were assessed by their incubation with mytilus galloprovincialis' hemolymph and subcellular fraction of gills, respectively. aunps did not alter enzymatic activities unlike ionic gold that inhibited ache and gst activities at 2.5 and 0.42mg·l(-1), respectively. ...	2016	26988391
the mirna biogenesis in marine bivalves.	small non-coding rnas include powerful regulators of gene expression, transposon mobility and virus activity. among the various categories, mature micrornas (mirnas) guide the translational repression and decay of several targeted mrnas. the biogenesis of mirnas depends on few gene products, essentially conserved from basal to higher metazoans, whose protein domains allow specific interactions with dsrna. here, we report the identification of key genes responsible of the mirna biogenesis in 32 b ...	2016	26989613
transcriptomics provides insight into mytilus galloprovincialis (mollusca: bivalvia) mantle function and its role in biomineralisation.	the mantle is an organ common to all molluscs and is at the forefront of the biomineralisation process. the present study used the mediterranean mussel (mytilus galloprovincialis) as a model species to investigate the structural and functional role of the mantle in shell formation. the transcriptomes of three regions of the mantle edge (umbo to posterior edge) were sequenced using illumina technology which yielded a total of 61,674,325 reads after adapter trimming and filtering. the raw reads as ...	2016	27037218
killing of vibrio cholerae and escherichia coli strains carrying d-mannose-sensitive ligands by mytilus hemocytes is promoted by a multifunctional hemolymph serum protein.	in aquatic environments, bivalve mollusks represent an important ecological niche for microorganisms. persistence of bacteria in bivalve tissues partly depends on their capacity to survive the bactericidal activity of the hemolymph due to both cellular (hemocyes) and soluble serum factors (e.g., enzymes, lectins, opsonins). the extrapallial protein (ep) present in serum of mytilus galloprovincialis (mgep) has been recently shown to work as an opsonin promoting d-mannose sensitive (ms) interactio ...	2016	27041371
ecotoxicological potential of antibiotic pollution-industrial wastewater: bioavailability, biomarkers, and occurrence in mytilus galloprovincialis.	environmental pollution by pharmaceutical residues has become a major problem in many countries worldwide. however, little is known about the concentrations of pharmaceuticals in water sources in tunisia. residues in the natural environment have been of increasing concern due to their impact on bacteria resistance development and toxicity to natural communities and ultimately to public health. in this work, we collected the wastewater sample from a pharmaceutical industry, which specializes in t ...	2016	27109112
presence of trace metals in aquaculture marine ecosystems of the northwestern mediterranean sea (italy).	information regarding chemical pollutant levels in farmed fish and shellfish, along with the risks associated with their consumption is still scarce. this study was designed to assess levels of exposure to 21 trace elements in fish (dicentrarchus labrax), mussels (mytilus galloprovincialis) and oysters (crassostrea gigas) collected from aquaculture marine ecosystems of the northwestern mediterranean sea. metal concentrations showed great variability in the three species; the highest values of th ...	2016	27179326
polycyclic aromatic hydrocarbons in sediments and bivalves on the pacific coast of japan: influence of tsunami and fire.	surface sediments and at least one edible bivalve species (ruditapes philippinarum, mytilus galloprovincialis, and crassostrea gigas) were collected from each of seven intertidal sites in japan in 2013. the sites had experienced varying levels of tsunami and fire disturbance following the major earthquake of 2011. eight polycyclic aromatic hydrocarbons (pahs) were identified and analyzed by gas chromatography-mass spectrometry. total sediment pah concentration (ct), the sum of the average concen ...	2016	27232189
investigation of parasitic and viral pathogens in mussels (mytilus galloprovincialis) in the gulf of izmir, turkey.	to investigate microsporidia spp. parasite, hepatitis a virus (hav), and norovirus (nov) contamination in mussels collected from 8 stations in the inner, middle, and outer regions of the gulf of izmir.	2016	27279520
antiviral activity of myticin c peptide from mussel: an ancient defense against herpesviruses.	little is known about the antiviral response in mollusks. as in other invertebrates, the interferon signaling pathways have not been identified, and in fact, there is a debate about whether invertebrates possess antiviral immunity similar to that of vertebrates. in marine bivalves, due to their filtering activity, interaction with putative pathogens, including viruses, is very high, suggesting that they should have mechanisms to address these infections. in this study, we confirmed that constitu ...	2016	27307570
cloning and differential expression of a novel toll-like receptor gene in noble scallop chlamys nobilis with different total carotenoid content.	to investigate whether toll like receptors (tlrs) genes do have an immune influence on noble scallop chlamys nobilis under pathogen stress, acute challenges lasting 48 h to vibrio parahaemolyticus, lipopolysaccharide (lps), polyinosinic polycytidylic acid (poly i:c), and pbs were conducted in two scallop stains of orange and brown with different carotenoids content. a novel toll-like receptor gene called cntlr-1 was cloned and its transcripts under different challenges were determined. meantime, ...	2016	27403592
ceramide lipids in alive and thermally stressed mussels: an investigation by hydrophilic interaction liquid chromatography-electrospray ionization fourier transform mass spectrometry.	hydrophilic interaction liquid chromatography coupled to electrospray ionization-fourier transform mass spectrometry was employed to study ceramide lipids occurring in mussels of sp. mytilus galloprovincialis. lipid extracts from alive mussels and mussels deliberately subjected to specific thermal treatments were analyzed. in particular, single and tandem ms measurements were performed on a hybrid quadrupole-orbitrap mass spectrometer and then complemented by ms(n) analyses (n = 2, 3) achieved b ...	2016	27479706
genetic diversity of arcobacter isolated from bivalves of adriatic and their interactions with mytilus galloprovincialis hemocytes.	the human food-borne pathogens arcobacter butzleri and a. cryaerophilus have been frequently isolated from the intestinal tracts and fecal samples of different farm animals and, after excretion, these microorganisms can contaminate the environment, including the aquatic one. in this regard, a. butzleri and a. cryaerophilus have been detected in seawater and bivalves of coastal areas which are affected by fecal contamination. the capability of bivalve hemocytes to interact with bacteria has been ...	2017	27650799
spatial and temporal distribution of mercury and methylmercury in bivalves from the french coastline.	marine mercury (hg) concentrations have been monitored in the french coastline for the last half a century using bivalves. the analyses presented in this study concerned 192 samples of bivalves (mussels: mytilus edulis and mytilus galloprovincialis and oysters: crassostrea gigas and isognomon alatus) from 77 sampling stations along the french coast and in the french antilles sea. the goals of this study were to assess mehg levels in various common bivalves from french coastline, and to identify ...	2017	27745976
a bioinformatics approach reveals seven nearly-complete rna-virus genomes in bivalve rna-seq data.	viral metagenomics (viromics) can provide a great contribution in expanding the knowledge of viruses and the relationship with their hosts. viromic studies on marine organisms are still at a very early stage and only little efforts have been spent in the identification of viruses associated to marine invertebrates to date, leaving the complexity of marine viromes associated to bivalve hosts almost completely unexplored. however, the potential use of viromic approaches in the management of viral ...	2016	27769778
detection of arcobacter spp. in mytilus galloprovincialis samples collected from apulia region.	the aim of the study was to evaluate the occurrence of arcobacter spp. in 20 samples of mytilus galloprovincialis purchased at fish markets in apulia region. the detection of arcobacter spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mccd) agar supplemented with cefoperazone, amphotericin b and teicoplanin (cat). in 6 out of the 20 tested samples the presence of arcobacter spp. was found and confirmed by genus-based polymerase chain reaction. all t ...	2015	27800382
prevalence of foodborne viruses in mussels in southern italy.	in this study, the prevalence of various enteric viruses in mytilus galloprovincialis (mediterranean mussel) belonging to class a and class b mollusc-harvesting areas in the campania region in southern italy was evaluated. one hundred and eight mussels were analysed using real-time reverse transcription pcr during a 2-year collection period (2014-2015) to detect the following viruses: human norovirus (genogroups i and ii), rotavirus, astrovirus, sapovirus, aichivirus, hepatitis a virus and hepat ...	2017	28054332
evidence of freshwater algal toxins in marine shellfish: implications for human and aquatic health.	the occurrence of freshwater harmful algal bloom toxins impacting the coastal ocean is an emerging threat, and the potential for invertebrate prey items to concentrate toxin and cause harm to human and wildlife consumers is not yet fully recognized. we examined toxin uptake and release in marine mussels for both particulate and dissolved phases of the hepatotoxin microcystin, produced by the freshwater cyanobacterial genus microcystis. we also extended our experimental investigation of particula ...	2016	28073507
interspecies comparison of the mechanical properties and biochemical composition of byssal threads.	several bivalve species produce byssus threads to provide attachment to substrates, with mechanical properties highly variable among species. here, we examined the distal section of byssal threads produced by a range of bivalve species (mytilus edulis, mytilus trossulus, mytilus galloprovincialis, mytilus californianus, pinna nobilis, perna perna, xenostrobus securis, brachidontes solisianus and isognomon bicolor) collected from different nearshore environments. morphological and mechanical prop ...	2017	28153979
antifouling potential of nature-inspired sulfated compounds.	natural products with a sulfated scaffold have emerged as antifouling agents with low or nontoxic effects to the environment. in this study 13 sulfated polyphenols were synthesized and tested for antifouling potential using the anti-settlement activity of mussel (mytilus galloprovincialis) plantigrade post-larvae and bacterial growth inhibition towards four biofilm-forming bacterial strains. results show that some of these nature-inspired compounds were bioactive, particularly rutin persulfate ( ...	2017	28205590
responses of mytilus galloprovincialis hemocytes to environmental strains of vibrio parahaemolyticus, vibrio alginolyticus, vibrio vulnificus.	marine bivalves are exposed to different types of bacteria in the surrounding waters, in particular of the vibrio genus. in the hemocytes of the mussel mytilus spp. immune responses to different vibrios have been largely characterized. however, little information is available on the hemocyte responses to human pathogenic vibrios commonly detected in coastal waters and bivalve tissues that are involved in seafood-borne diseases.	2017	28390964