Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| intracellular ion activities in malpighian tubule cells of rhodnius prolixus: evaluation of na+-k+-2cl- cotransport across the basolateral membrane. | intracellular ion activities (a(ion)) and basolateral membrane potential (vbl) were measured in malpighian tubule cells of rhodnius prolixus using double-barrelled ion-selective microelectrodes. in saline containing 103 mmol l(-1) na+, 6 mmol l(-1) k+ and 93 mmol l(-1) cl-, intracellular ion activities in unstimulated upper malpighian tubules were 21, 86 and 32 mmol l(-1), respectively. in serotonin-stimulated tubules, acl was unchanged, whereas ana increased to 33 mmol l(-1) and ak declined to ... | 2002 | 12000809 |
| rhodnius prolixus vitellin is composed of three different populations: comparison with vitellogenin. | rhodnius prolixus oocyte extracts were chromatographed on an ion exchange column in order to purify vitellin (vt). three vt heterogeneous populations were identified and named vt(1), vt(2), and vt(3) according to their order of elution from the column. the phosphate content of each population was determined, after lipid extraction, and a heterogeneous distribution was found: vt(1) being the less phosphorylated (50 mol p/mol protein) and vt(3) the heavily phosphorylated population (281 mol p/mol ... | 2002 | 12044487 |
| activity and residual effect of two formulations of lambdacyhalothrin sprayed on palm leaves to rhodnius prolixus. | the insecticidal activity and residual effect of two formulations of lambdacyhalothrin were evaluated with rhodnius prolixus; laboratory and field tests were conducted in the state of chiapas, mexico. the results indicate that the lethal concentrations of the active ingredient of sc (lc50 = 2.37 and lc90 = 8.5 mg, a.i./m2) were 4-8 times than those with the insecticide wp applied on r. prolixus bugs in palm leaves, a common building material for thatched roofs. other investigators in south ameri ... | 2002 | 12048565 |
| nitric oxide limits parasite development in vectors and in invertebrate intermediate hosts. | nitric oxide (no) possesses antiparasitic effects on both protozoa and metazoa in vertebrate definitive and intermediate hosts. inducible no limits parasite development also in rhodnius prolixus and anopheles stephensi, the natural vectors of human trypanosomiasis and malaria respectively, and in the snail biomphalaria glabrata, a natural invertebrate intermediate host of human schistosomiasis. therefore, no limits trypanosoma, plasmodium, and schistosoma development at all stages of the parasit ... | 2002 | 12049194 |
| effects of gamma irradiation on the development of trypanosoma rangeli in the vector rhodnius prolixus. | studies on the effects of gamma radiation on the infectivity of trypanosoma rangeli (strain h14) for the vector rhodnius prolixus revealed that (i) the ld(50) (lethal dose for 50% of bugs) for uninfected insects was 4147 rads; (ii) irradiated insects with a dose of 1200 rads subsequently infected with the flagellates exhibited a mortality of 45%, while uninfected irradiated insects showed a mortality of 5%, and infected nonirradiated insects exhibited 10% mortality; (iii) flagellates were presen ... | 2002 | 12095237 |
| dynamic development of trypanosoma cruzi in rhodnius prolixus: role of decapitation and ecdysone therapy. | decapitation and ecdysone therapy on the population dynamics of the trypanosoma cruzi dm28c clone in the stomach, small intestine and rectum of fifth-instar larvae of rhodnius prolixus were investigated. parasites were not found in the small intestine and rectum of decapitated insects after 10 days post-infection (p.i.). decapitated ecdysone-supplemented insects sustained the flagellate infection in both gut compartments. in the rectum, the population density of parasites increased 5-fold in ecd ... | 2002 | 12107464 |
| cyclic nucleotide-independent phosphorylation of vitellin by casein kinase ii purified from rhodnius prolixus oocytes. | in this study we show that vitellin (vt) phosphorylation in chorionated oocytes of rhodnius prolixus is completely inhibited by heparin (10 microg/ml), a classical casein kinase ii (ck ii) inhibitor. vt phosphorylation is not affected by modulators of cyclic nucleotide-dependent protein kinases such as c-amp (10 microm), h-8 (1 microm) and h-89 (0.1 microm). we have obtained a 3000-fold vt-free enriched preparation of ck ii. autophosphorylation of this enzyme preparation in the presence of (32)p ... | 2002 | 12110292 |
| oocyte fertilization triggers acid phosphatase activity during rhodnius prolixus embryogenesis. | acid phosphatase activity, previously identified in rhodnius prolixus oocytes, was studied during egg development. fertilized eggs exhibited a five fold increase of total acid phosphatase activity during the first days of development. in contrast non-fertilized oviposited eggs showed no activation of this enzyme. an optimum ph of 4.0 for pnpp hydrolysis in a saturable linear reaction and a strong inhibition by lysosomal acid phosphatase inhibitors such as naf (10 mm) and na(+)/k(+) tartrate (0.5 ... | 2002 | 12110294 |
| aggregation behaviour and interspecific responses in rhodnius prolixus stål. | the response to intra- and interspecific faecal assembling signals was tested in rhodnius prolixus. papers impregnated with excrement of r. prolixus induced the aggregation of larvae of this species, but also of those of triatoma infestans. however, faeces belonging to t. infestans were not able to assemble larvae of r. prolixus. on the other hand, there was no response of r. prolixus to putative chemical factors from their cuticle (footprints), in contrast to t. infestans. results are discussed ... | 2002 | 12118293 |
| identification and distribution of carbohydrate moieties on the salivary glands of rhodnius prolixus and their possible involvement in attachment/invasion by trypanosoma rangeli. | in the present study, fitc-labelled lectins (wga, con a, pna, hpa, and tpa) were utilized to investigate carbohydrate residues on the surface of rhodnius prolixus salivary glands. the results revealed that the salivary glands are rich in carbohydrate moieties and the diversity in binding pattern of particular lectins showed the presence of specific carbohydrate residues in the basal lamina, muscle, and cell layers of the glands. subsequently, the sugars detected on the salivary gland surface wer ... | 2002 | 12128049 |
| the impact of chagas disease control in latin america: a review. | discovered in 1909, chagas disease was progressively shown to be widespread throughout latin america, affecting millions of rural people with a high impact on morbidity and mortality. with no vaccine or specific treatment available for large-scale public health interventions, the main control strategy relies on prevention of transmission, principally by eliminating the domestic insect vectors and control of transmission by blood transfusion. vector control activities began in the 1940s, initiall ... | 2002 | 12219120 |
| evidence for crf-like and kinin-like peptides as neurohormones in the blood-feeding bug, rhodnius prolixus. | in rhodnius prolixus, the rapid post-feeding diuresis is under neurohormonal control. while serotonin has been demonstrated to be a diuretic neurohormone [j exp biol 156 (1991) 557], a peptide is also known to be involved. previously, we have demonstrated the presence of corticotropin releasing factor (crf)-like and kinin-like peptides in the central nervous system (cns) of 5th instar rhodnius [j exp biol 202 (1999) 2017; peptides 22 (2001) 161]. these peptides are present in neurohemal sites of ... | 2002 | 12431735 |
| amino acids modulate ion transport and fluid secretion by insect malpighian tubules. | insect haemolymph typically contains very high levels of free amino acids. this study shows that amino acids can modulate the secretion of ions and water by isolated malpighian tubules of rhodnius prolixus and drosophila melanogaster. secretion rates of rhodnius tubules in amino-acid-free saline increase after addition of serotonin to a peak value, then slowly decline to a plateau. addition of glutamine, glutamate or aspartate to such tubules increases secretion rates dramatically relative to th ... | 2003 | 12456699 |
| changes in salivary nitrophorin profile during the life cycle of the blood-sucking bug rhodnius prolixus. | the insect rhodnius prolixus is a hematophagous hemipteran that has five nymphal instars. fifth instar nymphs contain, in their salivary glands, four nitrophorins which have already been described in the literature (np1, np2, np3 and np4). two new hemeproteins were isolated and partially characterized from first instar nymphs. np2, that shows an anticoagulant activity, was also identified, but np1, np3 and np4 were not found. as these new hemeproteins have amino-terminal sequences clearly homolo ... | 2003 | 12459197 |
| inhibition of hemostasis by a high affinity biogenic amine-binding protein from the saliva of a blood-feeding insect. | the saliva of the blood-feeding insect rhodnius prolixus contains numerous pharmacologically active substances. included among these are a number of lipocalin proteins that bind various ligands important in hemostasis and inflammation. one such protein is a biogenic amine-binding protein (abp) that binds serotonin, epinephrine, and norepinephrine. based on amino acid alignments, it is most similar to the nitrophorin group of lipocalins found in the same insect species. physiologically, this prot ... | 2003 | 12464610 |
| characterization of lipophorin binding to the fat body of rhodnius prolixus. | in insects, lipids are transported by a hemolymphatic lipoprotein, lipophorin. the binding of lipophorin to the fat body of the hematophagous insect rhodnius prolixus was characterized in a fat body membrane preparation, obtained from adult females. for the binding assay, purified lipophorin was radiolabelled in the protein moiety (125i-hdlp), and it was shown that iodination did not affect the affinity of the membrane preparation for lipophorin. under incubation conditions used, lipophorin bind ... | 2002 | 12530208 |
| on the biosynthesis of rhodnius prolixus heme-binding protein. | the biosynthesis of rhodnius prolixus heme-binding protein (rhbp), which is present in the hemolymph and oocytes of rhodnius prolixus, was investigated. fat bodies of female insects incubated in vitro with 14c-leucine were able to synthesize and secrete 14c-rhbp to the culture medium. titrtion of synthesized rhbp with hemin showed that the protein secreted by the fat bodies is bound to heme, despite the presence of apo-rhbp in the hemolymph. the sequence of the rhbp cdna encodes a pre-protein of ... | 2002 | 12530221 |
| lipophorin interaction with the midgut of rhodnius prolixus: characterization and changes in binding capacity. | several classes of lipids are transported in insect hemolymph by lipophorin, a major hemolymphatic lipoprotein. the binding of lipophorin to the midgut of the hematophagous insect rhodnius prolixus was characterized in a midgut membrane preparation, using purified lipophorin radiolabelled in protein moiety ((125)i-hdlp). lipophorin specific binding to membranes achieved equilibrium after 30-40 min, was sensitive to ph, and was maximal at ph 7.0. in the presence of increasing concentrations of me ... | 2003 | 12650691 |
| isolation and characterization of a novel insect defensin from rhodnius prolixus, a vector of chagas disease. | an antimicrobial peptide belonging to the defensin family of small cationic peptides associated with innate immunity in insects was isolated from the hemolymph of rhodnius prolixus, a vector of chagas disease. this peptide, designated r. prolixus defensin a, was purified and sequenced. the active peptide contains 43 residues and aligns well with other insect defensins. however the pre-pro region of the sequence has little shared identity with other insect defensins. we have identified 3 isoforms ... | 2003 | 12650692 |
| parasite polymorphism may serve to enhance fitness in different host environments. | phenotypic expression of trans-sialidase (ts) by trypanosoma cruzi (silvio strain) is restricted to a subpopulation (20-30%) of broad trypomastigotes (ts+ parasites), while the larger subpopulation of slender trypomastigotes lacks ts expression (ts- parasites). rhodnius prolixus nymphs were infected with fractionated t. cruzi parasite populations (silvio strain). bugs ingesting ts- parasites produced significantly higher parasite loads than bugs ingesting either ts+ parasites or unfractionated p ... | 2002 | 12656128 |
| prevention of the transmission of chagas' disease with pyrethroid-impregnated materials. | interrupting the transmission of chagas' disease using insecticide-treated materials could be a cost-effective option, particularly for sylvatic vectors, which enter houses at night. a randomized trial was undertaken that included all houses in two communities in regions endemic for this disease, one in venezuela (50 houses) and one in colombia (47 houses). after a baseline study (including a short questionnaire survey, entomologic assessment, and chagas' disease serology), each household was ra ... | 2003 | 12685636 |
| geographic distribution and morphometric differentiation of triatoma nitida usinger 1939 (hemiptera: reduviidae: triatominae) in guatemala. | triatoma nitida was found in 14 (0.4%) out of 3,726 houses located in six departments across guatemala, which were surveyed from 1994 to 1998 by the man-hour collection method. compared to previous information, the distribution of t. nitida in guatemala has increased from five to nine departments; the species is present in mild climates at altitudes from 960 to 1,500 m. fourteen percent of the intradomestic t. nitida were infected with trypanosoma cruzi. the species was often found in conjunctio ... | 2003 | 12700860 |
| lysophosphatidylcholine acts as an anti-hemostatic molecule in the saliva of the blood-sucking bug rhodnius prolixus. | blood-sucking arthropods possess a variety of anti-hemostatic factors in their salivary glands to maintain blood fluidity during feeding. in this work we demonstrate the anti-hemostatic properties of lysophosphatidylcholine (lysopc) isolated from the salivary glands of rhodnius prolixus. first, we examined salivary glands of fourth and fifth instar nymphs for their phospholipid composition. the lumen displayed an accumulation of its phospholipid content, mainly phosphatidylcholine and lysopc, wi ... | 2003 | 12740385 |
| molecular phylogeography of the amazonian chagas disease vectors rhodnius prolixus and r. robustus. | the phylogeographical structure of the closely related species rhodnius prolixus and r. robustus is presented based on a 663-base pair (bp) fragment of the mitochondrial cytochrome b gene. twenty haplotypes were recovered from 84 samples examined, representing 26 populations from seven latin american countries. the resulting phylogenetic tree is composed of five major reciprocally monophyletic clades, one representing r. prolixus and four representing r. robustus. while r. prolixus is a very hom ... | 2003 | 12753218 |
| impact of residual spraying on rhodnius prolixus and triatoma dimidiata in the department of zacapa in guatemala. | as a vector control program to control chagas disease in guatemala, residual spraying of rhodnius prolixus and triatoma dimidiata was performed, and its impact was measured in the department of zacapa. in order to identify infested villages and determine the degree of infestation, a baseline entomological survey to identify municipalities infested with vectors followed by an additional vector survey in areas known to be infested was conducted. residual spraying using pyrethroid insecticides was ... | 2003 | 12764447 |
| the effects of aposymbiosis and of an infection with blastocrithidia triatomae (trypanosomatidae) on the tracheal system of the reduviid bugs rhodnius prolixus and triatoma infestans. | we have investigated the effects of the absence of symbionts and of infection with a trypanosome on the tracheal supply to different organs in larvae of the blood-sucking bugs, rhodnius prolixus and triatoma infestans. in bugs grown without symbionts there were extensive reductions in the tracheal supply to all the internal organs examined. these bugs also excreted less fluid after blood meals, perhaps because of a reduction in the oxygen supply to the malpighian tubules. inclusion of vitamin b ... | 1998 | 12769885 |
| the interplay between patency, microsomal na(+)/k(+) atpase activity and juvenile hormone, in tenebrio molitor parasitized by hymenolepsis diminuta. | beetles infected with metacestodes of the rat tapeworm, hymenolepis diminuta, exhibit reduced fecundity, due to alterations in vitellogenesis. follicle cell patency is retarded and inefficient vitellogenin uptake ensues. here, we have reassessed patency and its stimulation by jh iii at day 3 post-infection, when the most detrimental changes are observed in other ovarian processes. in rhodnius prolixus, patency is believed to be brought about by the action of a jh-dependent membrane-bound na(+)/k ... | 1997 | 12769895 |
| role of the head in the ultrastructural midgut organization in rhodnius prolixus larvae: evidence from head transplantation experiments and ecdysone therapy. | studies on the effects of decapitation, head transplantation and ecdysone therapy on the ultrastructural organization of the midgut in 5th-instar larvae of rhodnius prolixus, were carried out. control insects had a typical and significant organization of the epithelial cells (mainly microvilli, extracellular membrane layers and basal portion of the epithelial cells) of the midgut (stomach and intestine) during the entire period of the experiment. however, the host larvae, when decapitated 1 day ... | 1998 | 12769937 |
| anti-diuresis in the blood-feeding insect rhodnius prolixus stål: antagonistic actions of camp and cgmp and the role of organic acid transport. | secretion of primary urine by upper malpighian tubules of the blood-sucking insect rhodnius prolixus has recently been shown to be inhibited by cyclic gmp (cgmp). in the present work, we have demonstrated that cgmp has effects antagonistic to those of camp in rhodnius tubules and have further characterized the effects of cgmp on tubular secretion. cyclic gmp inhibited secretion at all concentrations from 5x10(-6) to 10(-3)m, though this inhibition was partially or wholly reversed by large (2mm) ... | 1998 | 12769938 |
| ecdysteroidogenic action of bombyx prothoracicotropic hormone and bombyxin on the prothoracic glands of rhodnius prolixus in vitro. | an in-vitro assay for ecdysteroid synthesis by the prothoracic glands (pgs) of fifth instar rhodnius prolixus has been employed to evaluate the actions of prothoracicotropic neuropeptides from the silkmoth, bombyx mori. crude prothoracicotropic hormone (ptth) extracts of recently emerged adult brain complexes of bombyx induced a dose-dependent stimulation of ecdysteroid synthesis by rhodnius pgs, which was similar to that obtained using crude rhodnius ptth. in both cases, maximum stimulation was ... | 1997 | 12769976 |
| kcl reabsorption by the lower malpighian tubule of rhodnius prolixus: inhibition by cl(-) channel blockers and acetazolamide. | iono- and osmoregulation by the blood-feeding hemipteran rhodnius prolixus involves co-ordinated actions of the upper and lower malpighian tubules. the upper tubule secretes ions (na(+), k(+), cl(-)) and water, whereas the lower tubule reabsorbs k(+) and cl(-) but not water. the extent of kcl reabsorption by the lower tubule in vitro was monitored by ion-selective microelectrode measurement of cl(-) and/or k(+) concentration in droplets of fluid secreted by malpighian tubules isolated under oil. ... | 1997 | 12769977 |
| ion physiology of vitellogenic follicles. | the ion physiology of vitellogenic follicles from a lepidopteran (hyalophora cecropia) and a hemipteran (rhodnius prolixus) are compared. similarities that can be expected to occur in vitellogenic follicles of many other insects include: (1) gap junctions, which unite the cells of a follicle into an integrated electrical system, (2) transmembrane k(+) and h(+) gradients that account for over 60% of follicular membrane potentials, (3) absence of a cl(-) potential, (but the opening of channels to ... | 2002 | 12770038 |
| feeding is not necessary for triggering plasticization of the abdominal cuticle in haematophagous bugs. | a simple device was used to quantify changes in the mechanical properties of the cuticle of rhodnius prolixus and triatoma infestans that take place when these insects feed, i.e., plasticization. different stimuli were presented for 1min to test for their ability to trigger plasticization. these were: a blood meal, a ringer solution meal, contact with a warm surface and thermal stimulation without such contact. our results supplant any previous hypotheses that have supposed that the presence of ... | 1998 | 12770155 |
| the distribution and function of serotonin in the large milkweed bug, oncopeltus fasciatus. a comparative study with the blood-feeding bug, rhodnius prolixus. | the blood-feeding hemipteran, rhodnius prolixus, ingests a large blood meal at the end of each larval stage. to accommodate and process this meal, its cuticle undergoes plasticisation, and its gut and malpighian tubules respectively absorb and secrete a large volume of water and salts for rapid diuresis. serotonin has been found to be integral to the feeding process in this animal, along with a diuretic peptide(s). the large milkweed bug, oncopeltus fasciatus, tends to feed in a more continuous ... | 1999 | 12770278 |
| immune responses in rhodnius prolixus: influence of nutrition and ecdysone. | starved larvae of rhodnius prolixus, when challenged with enterobacter cloacae b12, had their mortality related to their period of starvation. r. prolixus larvae fed on plasma alone, compared with insects fed on whole blood, had their immune reactivity affected as shown by: (i) a significant reduction in the ability to produce cecropin-like and lysozyme activities in the haemolymph when inoculated with e. cloacae; (ii) a reduction in numbers of haemocytes and nodule formation following challenge ... | 1997 | 12770413 |
| wing shape divergence between rhodnius prolixus from cojedes (venezuela) and rhodnius robustus from mérida (venezuela). | the existence of rhodnius robustus as a species distinct from rhodnius prolixus has long been the main epidemiological question about chagas disease transmission in venezuela and surrounding countries. these two taxa are morphologically and genetically very similar, but only r. prolixus is assumed to colonize houses and transmit chagas disease to humans. r. robustus is assumed to be an exclusively sylvatic species, restricted to palm trees. if robustus and prolixus are actually the same species, ... | 2002 | 12797988 |
| transformation of rhodococcus rhodnii, a symbiont of the chagas disease vector rhodnius prolixus, with integrative elements of the l1 mycobacteriophage. | elimination of vector populations through the use of insecticides is the principal means of controlling chagas disease. because of the limitations of insecticide use, we have been developing a new potential method of control, to be used in conjunction with insecticide programs, a method which utilizes genetically modified symbiotic bacteria. these transformed bacteria can express anti-parasitic agents in the gut of the bug where the trypanosomes also are found. previous studies have shown that i ... | 2003 | 12809804 |
| anti-complement activity in the saliva of phlebotomine sand flies and other haematophagous insects. | the saliva of haematophagous insects has a series of pharmacological activities which may favour blood feeding. in the present study, an inhibitory effect on the complement system was observed in salivary extracts obtained from the phlebotomine sand flies lutzomyia longipalpis and lu. migonei. saliva from lu. longipalpis was capable of inhibiting both the classical and alternative pathways, while that from lu. migonei acted only on the former. other haematophagous insect species were screened fo ... | 2003 | 12885192 |
| epidemiology of chagas disease in guatemala: infection rate of triatoma dimidiata, triatoma nitida and rhodnius prolixus (hemiptera, reduviidae) with trypanosoma cruzi and trypanosoma rangeli (kinetoplastida, trypanosomatidae). | a five-year domiciliary collection in the 22 departments of guatemala showed that out of 4,128 triatomines collected, 1,675 were triatoma dimidiata (latreille, 1811), 2,344 were rhodnius prolixus stal 1859, and only 109 were t. nitida usinger 1939. the chagas disease parasite, trypanosoma cruzi, was found in all three species. their natural infection rates were similar in the first two species (20.6%; 19.1%) and slightly lower in t. nitida(13.8%). however there was no significant difference in t ... | 2003 | 12886407 |
| changes in the rate of co2 release following feeding in the insect rhodnius prolixus. | we describe for the first time changes in the rate of co2 release (as a surrogate of metabolic rate) in the terminal larval stage of the insect rhodnius prolixus following a blood meal and during the molt leading to the adult stage. these data are presented on a whole-animal basis as well as per gram wet and dry weight. we have also used techniques that allow us to describe the rate of release per gram of actual body tissue (i.e., removing the weight of the remaining bloodmeal in the gut and the ... | 2013 | 12905116 |
| pyrethroid-impregnated curtains for chagas' disease control in venezuela. | the use of insecticide treated materials for the control of chagas' disease transmission is potentially a cost-effective and sustainable option where vectors are sylvatic. a study was undertaken in two rural chagas' disease endemic communities (96 households) of trujillo state, venezuela. after a baseline study (including a short questionnaire survey, assessment of housing conditions, vector behaviour and preferences in vector protection), 37 households were chosen at random for protection by im ... | 2003 | 12943974 |
| [the proboscis of rhodnius prolixus and its function]. | 1952 | 13040097 | |
| [allergic reactions to the sting of reduvidoidea, rhodnius prolixus]. | 1954 | 13230817 | |
| histamine and histidine in excreta of the blood-sucking bug rhodnius prolixus. | 1956 | 13358716 | |
| [analysis of the blood-sucking act of bug rhodnius prolixus]. | 1956 | 13411782 | |
| [morphological and neuro-physiological analysis of bites of rhodnius prolixus stål 1858]. | 1957 | 13424388 | |
| arthropod transmission of rabbit papillomatosis. | the virus of rabbit papillomatosis, a neoplastic disease studied intensively in relation to cancer because of its tendency toward malignant transformation, has been successfully transmitted by aedes aegypti, rhodnius prolixus, and triatoma infestans from papillomas induced in cottontail rabbits, and by the first species mentioned, from papillomas induced in domestic rabbits. this was accomplished by interrupted feeding, feeding after an interval of several days from the infective meal, or by app ... | 1958 | 13549638 |
| [data on trypanosoma rangeli tejera, 1920, i. technic for obtaining it from the salivary gland of rhodnius prolixus]. | 1958 | 13586728 | |
| synthesis of thiamine and folic acid by nocardia rhodnii, the micro-symbiont of rhodnius prolixus. | 1960 | 13711570 | |
| [preliminary note on a new neotropic microphymenoptera of the scelionidae family, parasite of eggs of rhodnius prolixus (stal)]. | 2017 | 13731333 | |
| [study of the proteins of the hemolymph of triatoma infestans and rhodnius prolixus by ultra-microelectrophoresis in agar gel]. | 1959 | 13798984 | |
| [the influence of intestinal symbionts of rhodnius prolixus on trypanosoma cruzi]. | 1959 | 13856183 | |
| studies of the amino acids of rhodnius prolixus i. analysis of the haemolymph. | 1961 | 13904705 | |
| studies of the amino acids of rhodnius prolixus ii. analysis of the excretory material. | 1961 | 13904706 | |
| [on the mode of oviposition of triatoma megista and rhodnius prolixus]. | 2006 | 13943306 | |
| [survey of extra-domiciliary rhodnius prolixus in venezuela. (preliminary report)]. | 1963 | 13946326 | |
| [new aspects of the xenodiagnostic method using rhodnius prolixus]. | 2011 | 14056690 | |
| [verification of extradomestic rhodnius prolixus in venezuela. preliminary report]. | 1963 | 14112052 | |
| excretion in the blood-sucking bug, rhodnius prolixus stal. ii. the normal course of diuresis and the effect of temperature. | 1964 | 14161607 | |
| anticoagulant and fibrinolytic activities from rhodnius prolixus stal. | 1964 | 14191567 | |
| increased infectivity of a cyclically maintained strain of trypanosoma rangeli to rhodnius prolixus and mode of transmission by invertebrate host. | 1964 | 14215475 | |
| excretion in the blood-sucking bug, rhodnius prolixus stal. 3. the control of the release of the diuretic hormone. | 1964 | 14221042 | |
| mass rearing of rhodnius prolixus. | 1964 | 14272468 | |
| the gorging response in rhodnius prolixus stahl. | 1965 | 14287033 | |
| [development of a new method for the evaluation of house infestation by rhodnius prolixus]. | 1965 | 14334652 | |
| a simple apparatus for the artificial feeding of rhodnius prolixus (hemiptera, reduviidae). | 1960 | 14399808 | |
| studies on rhodnius prolixus: growth and development of normal and sterile bugs, and the symbiotic relationship. | 1960 | 14399809 | |
| inhibition of the sapk/jnk pathway blocks the stimulatory effects of glutamine on fluid secretion by the malpighian tubules of rhodnius prolixus. | physiological levels of amino acids such as glutamine, glutamate, aspartate and proline increase the rates of fluid secretion and ion transport by serotonin-stimulated malpighian tubules (mts) of rhodnius prolixus. here, we examine the proposal that the effects of glutamine are mediated through activation of specific kinases to produce the observed increases in fluid secretion. the glutamine-dependent increase in mt fluid secretion rate was blocked by two chemically unrelated inhibitors of the s ... | 2003 | 14511822 |
| a miniature radioimmunoassay for melatonin for use with small samples from invertebrates. | melatonin has been identified in a number of invertebrates, but little is known of its functions or its circadian control. studies in this area are hindered by the lack of an assay for melatonin that is appropriate for use with the small sample volumes obtainable from most invertebrates and with the large numbers of sequential samples generated by circadian studies. we describe and validate a novel, miniature radioimmunoassay (ria) protocol for these purposes. sample volumes of c.50 microl were ... | 2003 | 14511991 |
| further morphological studies on the behavior of trypanosoma rangeli in the hemocytes of rhodnius prolixus. | the hemocytes of rhodnius prolixus were analyzed during the course of infection with the protozoan trypanosoma rangeli. the following cell types were identified: prohemocyte, plasmatocyte, adipocyte, granular cell and oenocytoid. the number of these cells changes during the infection course thus indicating a cell response to infection of r. prolixus by t. rangeli. transmission electron microscopy showed that plasmatocytes were able to ingest epimastigote forms of the parasite, which were then fo ... | 2003 | 14665386 |
| axial ligand complexes of the rhodnius nitrophorins: reduction potentials, binding constants, epr spectra, and structures of the 4-iodopyrazole and imidazole complexes of np4. | previously, we utilized 4-iodopyrazole (4ipzh) as a heavy atom derivative for the initial solution of the crystal structure of the nitrophorin from rhodnius prolixus, np1, where it was found to bind to the heme with the iodo group disordered in two positions. we have now determined the structure of the 4ipzh complex of np4 at ph 7.5 and find that the geometry and bond lengths at the iron center are extremely similar to those of the imidazole (imh) complex of the same protein (structure determine ... | 2004 | 14673714 |
| the chagas vector, triatoma dimidiata (hemiptera: reduviidae), is panmictic within and among adjacent villages in guatemala. | trypanosoma cruzi, the hemoflagellate parasite and cause of chagas disease in latin america, is carried by triatomine vectors, principally triatoma dimidiata and rhodnius prolixus in central america. to assist control efforts and to understand the epidemiology of the disease in guatemala, the population genetics of t. dimidiata was analyzed among three houses within a village and two adjacent villages in guatemala. eleven randomly amplified polymorphic dna-polymerase chain reaction (rapd-pcr) pr ... | 2003 | 14680107 |
| the distribution and effects of dippu-allatostatin-like peptides in the blood-feeding bug, rhodnius prolixus. | using a polyclonal antiserum to dippu-allatostatin 7 (dippu-ast 7; formerly ast 1) of the cockroach diploptera punctata, we have demonstrated the presence of ast-like immunoreactivity (ali) in cells and processes throughout the nervous system, gut, and peripheral tissues of unfed fifth instar and adult rhodnius prolixus. ali in apparent neurosecretory cells of the brain, suboesophageal ganglion, and mesothoracic ganglionic mass, as well as in midgut endocrine cells, suggests that rhodnius allato ... | 2003 | 14706534 |
| a new model for proton pumping in animal cells: the role of pyrophosphate. | the h+-ppase activity was characterized in membrane fractions of ovary and eggs of rhodnius prolixus. this activity is totally dependent on mg2+, independent of k+ and strongly inhibited by naf, idp and ca2+. the membrane proteins of eggs were analyzed by western blot using antibodies to the h+-ppase from arabidopsis thaliana. the immunostain was associated with a single 65-kda polypeptide. this polypeptide was immunolocalized in yolk granule membranes by optical and transmission electron micros ... | 2004 | 14723894 |
| temperature preference in rhodnius prolixus, effects and possible consequences. | the present work examines the thermal preference of adult rhodnius prolixus along a temperature gradient. the mean preferred temperature differed slightly between sexes: 25.0 degrees c for males versus 25.4 degrees c for females. this preference was not constant, but varied daily by about 0.2 degrees c for both sexes, and reached its highest value at the onset of the dark phase and was lowest during the light phase. a change in the preferred temperature with the level of starvation was also obse ... | 2004 | 14739030 |
| spermatophore production and egg-laying behaviour in rhodnius prolixus stal. (hemiptera; reduviidae). | 1950 | 14785969 | |
| identification and tissue-specific distribution of sulfated glycosaminoglycans in the blood-sucking bug rhodnius prolixus (linnaeus). | we have previously characterized heparan sulfate (hs) as the major ovarian sulfated glycosaminoglycan (gag) in females of rhodnius prolixus, while chondroitin sulfate (cs) was the minor component. using histochemical procedures we found that gags were concentrated in the ovarian tissue but not found inside the oocytes. here, we extend our initial observations of gag expression in r. prolixus by characterizing these molecules in other organs: the fat body, intestinal tract, and the reproductive t ... | 2004 | 14871621 |
| exploring the sialome of the blood-sucking bug rhodnius prolixus. | rhodnius prolixus is a hemiptera that feeds exclusively on vertebrate blood in all life stages. its salivary glands produce potent pharmacological substances that counteract host hemostasis, including anti-clotting, anti-platelet, and vasodilatory substances. to obtain a further insight into the salivary biochemical and pharmacological complexity of this insect, a cdna library was randomly sequenced, and salivary gland homogenates were fractionated by hplc to obtain aminoterminal sequences of ab ... | 2004 | 14976983 |
| effects of eicosanoid biosynthesis inhibitors on the prophenoloxidase-activating system and microaggregation reactions in the hemolymph of rhodnius prolixus infected with trypanosoma rangeli. | investigations on the effects of eicosanoid biosynthesis inhibitors on the hemocyte microaggregation and prophenoloxidase (propo)-activating system in the hemolymph, parasitemia and mortality of rhodnius prolixus infected with trypanosoma rangeli were performed. hemocoelic injection of live t. rangeli epimastigotes into fifth-instar larvae of r. prolixus that previously fed on blood containing an inhibitor of phospholipase a(2) (dexamethasone), a specific inhibitor of the cyclooxygenase pathway ... | 2004 | 15019517 |
| changes associated with laboratory rearing in antennal sensilla patterns of triatoma infestans, rhodnius prolixus, and rhodnius pallescens (hemiptera, reduviidae, triatominae). | we examined changes in the array of antennal sensilla of three species of triatominae (triatoma infestans, rhodnius prolixus, and r. pallescens) following their establishment for different periods in laboratory culture. in each case, the laboratory colonies were compared with conspecific samples taken directly from the field, by quantitative analysis of the sensilla arrays on the three distal segments of the antenna in terms of the densities of three types of chemoreceptors (basiconics and thick ... | 2004 | 15057343 |
| role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4. | nitrophorins are ferric heme proteins that transport nitric oxide (no) from blood-sucking insects to victims. no binding is tighter at lower ph values, as found in the insect salivary gland, and weaker at the ph of the victim's tissue, facilitating no release and subsequent vasodilation. previous structural analyses of nitrophorin 4 (np4) from rhodnius prolixus revealed a substantial no-induced conformational change involving the a-b and g-h loops, which rearrange to desolvate the distal pocket ... | 2004 | 15157102 |
| inhibition of hemocyte microaggregation reactions in rhodnius prolixus larvae orally infected with trypanosoma rangeli. | hemocoelic inoculation of epimastigotes of trypanosoma rangeli strain h14 into 5th-instar larvae of rhodnius prolixus previously fed on blood containing the same parasites, showed reduced number of hemocyte microaggregates in the hemolymph, enhanced number of flagellates in the hemolymph as well as increased mortality of these insects. all these effects were counteracted by combined inoculation of r. prolixus with t. rangeli and arachidonic acid. in vitro assays using hemolymph taken from insect ... | 2004 | 15208035 |
| isolation of serratia marcescens in the midgut of rhodnius prolixus: impact on the establishment of the parasite trypanosoma cruzi in the vector. | the effects of resident bacteria in the stomach of 5th-instar larvae of rhodnius prolixus on the erythrocyte lysis and trypanosoma cruzi infection were studied. the bacteria population increased approximately 10,000-fold after feeding. hemolysis rose to approximately 28% within 24h postfeeding and then linearly grew until day 4 attaining almost 100%. the number of surviving y strain of t. cruzi in the stomach declined drastically, while the infection with dm28c clone was maintained stable. five ... | 2004 | 15208042 |
| extracellular h+ dynamics during oogenesis in rhodnius prolixus ovarioles. | the spatiotemporal dynamics of transmembrane hydrogen ion (h+) fluxes during oogenesis were investigated in the telotrophic ovarioles of the insect, rhodnius prolixus. although rhodnius ovarioles possess exaggerated morphological and electrical polarity between nurse cells and oocytes, little is known about h+ changes during oogenesis, despite the regulatory role played by h+ and ph in many relevant cellular processes. a number of dynamic extracellular h+ fluxes were measured along rhodnius ovar ... | 2004 | 15235012 |
| structural dynamics controls nitric oxide affinity in nitrophorin 4. | nitrophorin 4 (np4) is one of seven nitric oxide (no) transporting proteins in the blood-sucking insect rhodnius prolixus. in its physiological function, no binds to a ferric iron centered in a highly ruffled heme plane. carbon monoxide (co) also binds after reduction of the heme iron. here we have used fourier transform infrared spectroscopy at cryogenic temperatures to study co and no binding and migration in np4, complemented by x-ray cryo-crystallography on xenon-containing np4 crystals to i ... | 2004 | 15258143 |
| lignoids in insects: chemical probes for the study of ecdysis, excretion and trypanosoma cruzi-triatomine interactions. | the effects of phytochemicals (lignans and neolignans) are reviewed in a variety of insect species with special focus on the recent advances on feeding, excretion and trypanosoma cruzi interactions with rhodnius prolixus. burchellin, podophyllotoxin, pinoresinol, sesamin, licarin a, and nordihydroguaiaretic acid (ndga) added to the diet of rhodnius prolixus larvae induce antifeedant effects only in doses up to 100 microg/ml of blood meal. additionally, pinoresinol and ndga significantly inhibit ... | 2004 | 15302525 |
| mortality profiles of rhodnius prolixus (heteroptera: reduviidae), vector of chagas disease. | life table data of rhodnius prolixus (heteroptera: reduviidae) kept at laboratory conditions were analysed in search for mortality patterns. gompertz and weibull mortality models seem adequate to explain the sigmoid shape of the survivorship curve. a significant fit was obtained with both models for females (r(2) = 0.70, p < 0.0005 for the gompertz model; r(2) = 0.78, p < 0.0005 for the weibull model) and for males (r(2) = 0.39, p < 0.0005 for the gompertz model; r(2) = 0.48, p < 0.0005 for the ... | 2004 | 15350863 |
| characterization of an ecto-phosphatase activity in malpighian tubules of hematophagous bug rhodnius prolixus. | we have characterized a phosphatase activity present on the external surface of intact malpighian tubules in rhodnius prolixus. this phosphatase hydrolyses the substrate p-nitrophenyl phosphate at a rate of 3.38 +/- 0.07 nmol pi x mg(-1) x min(-1). phosphatase activity decreased with the increase of the ph from 6.4 to 7.6 ph, a range in which tubules cellular integrity was maintained for at least 1 h. classical inhibitors of acid phosphatase, such as ammonium molybdate, fluoride, vanadate, mpv-p ... | 2004 | 15352154 |
| na+ competes with k+ in bumetanide-sensitive transport by malpighian tubules of rhodnius prolixus. | we examined the effects of bathing saline na+/k+ ratio, bumetanide and hydrochlorothiazide on fluid and ion transport by serotonin-stimulated malpighian tubules of rhodnius prolixus. previous pharmacological and electrophysiological studies indicate that a bumetanide-sensitive na+/k+/2cl- cotransporter is the primary route for basolateral ion entry into the cell during fluid secretion. the goal of this study was to resolve the apparent conflict between relatively high secretion rates by tubules ... | 2004 | 15371478 |
| chlorinated tyrosine derivatives in insect cuticle. | a method for quantitative measurement of 3-monochlorotyrosine and 3,5-dichlorotyrosine in insect cuticles is described, and it is used for determination of their distribution in various cuticular regions in nymphs and adults of the desert locust, schistocerca gregaria. the two chlorinated tyrosine derivatives were present in all analyzed regions in mature adult locusts, the highest concentrations were found in the sclerotized cuticle of femur and tibia, but significant amounts were also present ... | 2004 | 15475302 |
| rhodnius prolixus malpighian tubule's aquaporin expression is modulated by 5-hydroxytryptamine. | the purpose of the present study was to detect the presence of an aquaporin-like water channel, a member of the major intrinsic protein (mip) family, in the malpighian tubule (mt) of the hematophagous insect rhodnius prolixus, which excrete a large bulk of fluid after a massive blood meal, and its possible regulation by 5-hydroxytryptamine (5-ht). reverse transcription polymerase chain reaction (rt-pcr) and southern blots of cdna was obtained from adult r. prolixus mt poly (a)+ rna. employing de ... | 2004 | 15484262 |
| appetence behaviours of the triatomine bug rhodnius prolixus on a servosphere in response to the host metabolites carbon dioxide and ammonia. | a combination of 1,000 ppm co(2) plus 30-40 ppb nh(3) in an air stream induced rhodnius prolixus nymphs walking on a servosphere to perform a series of appetence behaviours. shortly after the onset of stimulation the nymphs turned sharply upwind towards the source of the chemostimuli (within 13 +/- 9 s) from mostly downwind and crosswind walks in the air stream alone. the mean vector angles of these upwind tracks were concentrated in a cone 60 degrees either side of due upwind. the upwind walkin ... | 2004 | 15503053 |
| protein functional cycle viewed at atomic resolution: conformational change and mobility in nitrophorin 4 as a function of ph and no binding. | the blood-sucking insect rhodnius prolixus uses nitrophorin 4, a heme protein, to deliver nitric oxide (no) to a victim, causing vasodilation and improved feeding. binding of no occurs at a ferric heme and is modulated by ph. no binding at lower ph induces a large conformational change involving loops a-b and g-h that leads to distal pocket desolvation and protection of the nitrosyl heme complex. we have determined the crystal structures of rhodnius nitrophorin 4 to ultrahigh resolution in four ... | 2004 | 15504026 |
| mixed domestic infestation by rhodnius prolixus stal, 1859 and panstrongylus geniculatus latreille, 1811, vector incrimination, and seroprevalence for trypanosoma cruzi among inhabitants in el guamito, lara state, venezuela. | mixed infestation of nymphs and adults of rhodnius prolixus stal, 1859 and panstrongylus geniculatus latreille, 1811 was detected in 3 (15%) of 20 dwellings in el guamito, an endemic focus of chagas disease in lara state, venezuela. in one of the houses, both species were positive for trypanosoma cruzi: 14.3% (r. prolixus) and 20% (p. geniculatus ). the overall infection rate in 143 of 352 r. prolixus was 16.1%. parasites isolated from r. prolixus were identified as t. cruzi i by random amplifie ... | 2004 | 15516649 |
| antihemostatic strategies of blood-feeding arthropods. | arthropods in at least 23 different families or orders, distributed between two classes (insecta and arachnida), feed on vertebrate blood. they are able to do this despite constraints imposed by a sophisticated array of hemostatic defenses, due to the presence of a wide range of antihemostatic molecules in their saliva, including vasodilators, antiplatelet factors, and anticoagulants. vasodilators include amines, prostaglandins, peptides, proteins, and even a mechanism to store large amounts of ... | 2004 | 15578959 |
| sulfated glycosaminoglycans in two hematophagous arthropod vectors of chagas disease, triatoma brasiliensis and rhodnius prolixus (hemiptera: reduviidae). | the characterization of sulfated glycosaminoglycans (gags) in hematophagous arthropod vectors in general has been limited, with the exception of the studies in the triatomine rhodnius prolixus. heparan sulfate (hs) and chondroitin sulfate (cs) were previously identified and structurally characterized in extracts of whole bodies of fourth instar larvae of r. prolixus. recently, we showed the expression of these two sulfated gags in specific body tissues of adult males and females and in embryos o ... | 2004 | 15581795 |
| nitric oxide interaction with insect nitrophorins and thoughts on the electron configuration of the {feno}6 complex. | the nitrophorins are no-carrying heme proteins that are found in the saliva of two species of blood-sucking insects, the kissing bug (rhodnius prolixus) and the bedbug (cimex lectularius). in both insects the no is bound to the ferric form of the protein, which gives rise to kds in the micromolar to nanomolar range, and thus upon injection of the saliva into the tissues of the victim the no can dissociate to cause vasodilation and inhibition of platelet aggregation. the structures of the protein ... | 2005 | 15598503 |
| presence and activity of a dippu-dh31-like peptide in the blood-feeding bug, rhodnius prolixus. | the blood-feeding bug, rhodnius prolixus, ingests large blood meals, then undergoes a period of rapid diuresis which is under neurohormonal control. in both cockroach (diploptera punctata) and fruit fly (drosophila melanogaster) a calcitonin-like dh31 neuropeptide has been identified [coast gm, webster sg, schegg km, tobe ss, schooley da. the drosophila melanogaster homologue of an insect calcitonin-like diuretic peptide stimulates v-atpase activity in fruit fly malpighian tubules. j exp biol 20 ... | 2005 | 15626502 |
| the distribution and activity of tachykinin-related peptides in the blood-feeding bug, rhodnius prolixus. | the invertebrate tachykinin-related peptides (trps) with the conserved c-terminal sequence fx1gx2ramide shows sequence similarity to the vertebrate tachykinins after which they are named, and are hypothesized to be ancestrally related. in this study a polyclonal antiserum generated against a locust tachykinin (lomtk i), was used to demonstrate the presence and describe the distribution of lomtk-like immnoreactivity in the cns and gut of rhodnius prolixus. reverse phase high performance liquid ch ... | 2005 | 15626503 |
| heme-assisted s-nitrosation of a proximal thiolate in a nitric oxide transport protein. | certain bloodsucking insects deliver nitric oxide (no) while feeding, to induce vasodilation and inhibit blood coagulation. we have expressed, characterized, and determined the crystal structure of the cimex lectularius (bedbug) nitrophorin, the protein responsible for no storage and delivery, to understand how the insect successfully handles this reactive molecule. surprisingly, no binds not only to the ferric nitrophorin heme, but it can also be stored as an s-nitroso (sno) conjugate of the pr ... | 2005 | 15637157 |