Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| does prematurity alter the course of respiratory syncytial virus infection? | we compared previously healthy prematurely born infants with full-term infants hospitalized with respiratory syncytial virus (rsv) infection to evaluate the role of prematurity on the clinical course of the illness. during a 5-yr period (1984 to 1989), 484 previously healthy patients were admitted to the hospital with rsv infection. no differences were found in the presenting symptoms of respiratory distress, cough, fever or shock, although the premature group was more likely to present with apn ... | 1990 | 2245609 |
| enhanced pulmonary histopathology is observed in cotton rats immunized with formalin-inactivated respiratory syncytial virus (rsv) or purified f glycoprotein and challenged with rsv 3-6 months after immunization. | formalin-inactivated (fi) rsv, purified f glycoprotein in alum, and rsv infection (intranasal) were compared for their immunogenicity, efficacy, and ability to enhance pulmonary histopathology during rsv infection 3 and 6 months following immunization by the intramuscular route. purified influenza virus in alum was used as a control immunogen. at 1 month following immunization with one dose of purified f glycoprotein (5 micrograms), cotton rats developed levels of f antibodies (elisa) higher tha ... | 1990 | 2251875 |
| evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus. | four methods of detecting respiratory syncytial virus (rsv) from clinical specimens were evaluated. a total of 410 specimens consisting of nasopharyngeal washes, aspirates, and swabs were simultaneously tested for the presence of rsv by direct immunofluorescence assay (dfa), enzyme immunoassay (eia) (kallestad pathfinder), shell vial centrifugation culture (svc), and conventional culture. dfa identified 146 (83%) of the 175 positive cases, eia detected 153 (87%), svc detected 127 (73%), and conv ... | 1990 | 2254415 |
| virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin e2. | the cellular immune response to respiratory syncytial virus (rsv) is felt to contribute to viral clearance and/or the inflammation accompanying pulmonary infections with this virus. both tumor necrosis factor (tnf) and prostaglandin e2 (pge2) are important regulatory mediators of the cellular immune response. we examined the production of these mediators from purified human alveolar and blood mononuclear phagocytes (mp) after rsv infection in vitro and compared production induced by virus with t ... | 1990 | 2260674 |
| identification of viruses in a study of acute respiratory tract infection in children from uruguay. | for the purpose of identifying viral agents associated with acute respiratory tract infections (ari) in children less than 5 years old, a longitudinal community study was undertaken in montevideo, uruguay, from may 1985 to december 1987. this report includes results obtained by cell culture and immunofluorescence techniques for detection of respiratory syncytial virus (rsv), influenza a and b viruses, parainfluenza 1 and 3 viruses, and adenovirus. two populations were studied: children visited a ... | 1990 | 2270420 |
| complementarity between the 5'- and 3'-terminal sequences of rice stripe virus rnas. | the 5' and 3' termini of four ssrna species of rice stripe virus (rsv) isolate t were sequenced. the 3' termini of the three smallest ssrnas, i.e. rnas 2, 3 and 4, had the sequence 5' gacuuugugu 3'; that of ssrna 1 had the sequence 5' gacuaugugu 3'. the 5'-terminal sequences of all four ssrnas were 5' acacaaagucc 3'. the 5'- and 3'-terminal sequences of about 20 bases of each ssrna were almost complementary to each other. it is possible that rsv rnas form panhandle structures characteristic of t ... | 1990 | 2273384 |
| the two open reading frames of the 22k mrna of human respiratory syncytial virus: sequence comparison of antigenic subgroups a and b and expression in vitro. | the sequence of the 22k mrna of strain 18537 of antigenic subgroup b of human respiratory syncytial virus (rsv) was determined by sequencing cloned cdnas of intracellular mrna. comparison with the corresponding sequence of the a2 strain of subgroup a showed that there is 78% nucleotide sequence identity overall, that the amino acid sequence of the 22k protein is 92% identical between subgroups and that the 22k mrna of both subgroups contains a second, internal, overlapping open reading frame (or ... | 1990 | 2273393 |
| [serologic response measured by complement fixation in children with acute respiratory infection]. | the aim of this work was to evaluate the sensitivity of complement fixation for serological diagnosis of lower acute respiratory infections (ari) in small children in comparison with direct methods such as indirect immunofluorescence (iif) on nasopharyngeal aspirate and tissue culture isolation. sera from children under 5 year of age with ari were studied by complement fixation for 6 respiratory viruses (respiratory syncytial virus (rsv), adenovirus, influenza a and b and parainfluenza 1 and 3). ... | 1990 | 2287713 |
| serodiagnosis of respiratory syncytial virus (rsv) infection in children as measured by detection of rsv-specific immunoglobulins g, m, and a with enzyme-linked immunosorbent assay. | the diagnostic value of an enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (rsv)-specific immunoglobulin g (igg), igm, and iga in sera from infants and children with proven rsv infection, from a control group, and from patients with symptoms of viral respiratory disease was analyzed. compared to virus isolation and rsv antigen detection methods, the sensitivity of this assay was 87% and the specificity was 79%. for igg alone, these were 45 and 92%, for igm alone th ... | 1990 | 2298874 |
| severe respiratory syncytial virus infection in older children. | serious respiratory syncytial virus (rsv) disease requiring hospitalization occurs primarily in infants younger than 12 months. the incidence, risk factors, and clinical features in older children have not been studied extensively. of 282 children hospitalized at our institution with severe rsv disease during a 3-year period, 62 (22%) were older than 12 months. these 62 older children were matched for sex, onset of illness, and hospital location with 62 hospitalized children younger than 12 mont ... | 1990 | 2305743 |
| refractory congestive heart failure after ribavirin in infants with heart disease and respiratory syncytial virus. | although treatment with ribavirin has been known to be associated with a decreased mortality in infants with congenital heart disease (chd) who have respiratory syncytial virus (rsv), few data are available regarding morbidity. we reviewed records of 10 consecutively hospitalized infants with chd during a recent rsv epidemic. despite the presence of left-to-right shunt in each patient, symptoms of rsv were respiratory at presentation. after ribavirin, decreased respiratory symptoms were found in ... | 1990 | 2308658 |
| a seroepidemiologic study of bovine respiratory syncytial virus (brsv) in turkey. | in this study, a survey of cattle for neutralizing antibodies to respiratory syncytial virus (rsv) in turkey is presented. 490 serum samples were collected from several state farms and some private flocks in different regions of turkey. the serologic examination was done by the microtiter serum-neutralization technique. in this report, a positive serum antibody titer at dilutions of 1:2 was demonstrated in 226 (46.12%) of the 490 sera tested. | 1990 | 2311535 |
| viral aetiology of acute respiratory infections in children in north india. | two hundred and thirty children clinically diagnosed as suffering from acute respiratory infection were tested for four major groups of viral aetiological agents, i.e. influenza para-influenza, respiratory syncytial virus (rsv) and adenoviruses using indirect immunofluorescence technique. at least one of the respiratory viruses was identified in 51 (22 per cent) specimens, which included influenza a in 6 (3 per cent), influenza b in 3 (1 per cent), para-influenza type 1 in 3 (1 per cent), para-i ... | 1990 | 2313777 |
| association between respiratory syncytial virus outbreaks and lower respiratory tract deaths of infants and young children. | the temporal patterns of respiratory virus isolations from 10 laboratories in the usa were compared with that of deaths of children less than 5 years old from july 1975 through june 1984. isolations of respiratory syncytial virus (rsv) occurred as yearly winter outbreaks; parainfluenza virus 1 and 2 isolations occurred as well-defined outbreaks every other year in the autumn; parainfluenza virus 3 isolations occurred throughout the year with periodic, increased isolations suggestive of outbreaks ... | 1990 | 2319164 |
| severe respiratory syncytial virus pneumonia in an adult renal transplant recipient: successful treatment with ribavirin. | respiratory syncytial virus (rsv) pneumonia may be a severe disease especially in immunocompromised patients. it is quite rare in adults. we report a case of severe rsv pneumonia in a 38-year-old male renal transplant recipient with successful outcome after ribavirin aerosol therapy. | 1990 | 2320966 |
| isolation of respiratory syncytial virus from liver tissue and extrahepatic biliary atresia material. | respiratory syncytial virus (rsv) was isolated from liver tissue in a 7-month-old infant with extrahepatic biliary atresia. to our knowledge this is the first report of rsv isolation from human liver tissue. | 1990 | 2320967 |
| chest x-ray appearances in pneumonia and bronchiolitis. correlation to virological diagnosis and secretory bacterial findings. | chest x-ray findings were related to virus diagnosis, age and secretory bacterial findings in 128 infants and children under 7 years of age with clinical pneumonia and bronchiolitis. they belonged to a cohort examined in connection with the introduction of rapid methods for virological diagnosis. seventy-six children had a virus infection diagnosed by examination of nasopharyngeal secretion and/or by serological methods. thirty-seven of these children were classified as having pathogenic bacteri ... | 1990 | 2321485 |
| evaluation in chimpanzees of vaccinia virus recombinants that express the surface glycoproteins of human respiratory syncytial virus. | the immunogenicity and protective efficacy of recombinant vaccinia viruses that express the two major protective antigens of human respiratory syncytial virus (rsv), the f and g glycoproteins, were evaluated in chimpanzees. in previous studies in rodents and monkeys the f and g proteins expressed by the same recombinants were highly immunogenic and induced high levels of resistance to rsv replication following subsequent challenge. in contrast, in chimpanzees, a single intradermal immunization i ... | 1990 | 2336876 |
| screening for respiratory syncytial virus and assignment to a cohort at admission to reduce nosocomial transmission. | to limit nosocomial spread of respiratory syncytial virus (rsv) infection, a longitudinal intervention trial was instituted. nasal secretions or washes were screened for rsv antigen by enzyme-linked immunosorbent assay, and patients were assigned to an rsv-infected or an rsv-uninfected cohort. the baseline (preintervention) rate of 7.17 nosocomial cases of rsv per 1000 patient-days of care was used for comparison. despite continued infections in the community after screening was initiated, there ... | 1990 | 2348292 |
| an outbreak of an influenza-like illness in a nursing home. | as part of an ongoing infection surveillance program, data were collected on an outbreak of respiratory illness affecting 29% of residents in a 231-bed long-term care facility. viral titers could be collected on 60% of the ill residents and respiratory syncytial virus (rsv) was established in 14 of the symptomatic patients and was thought to be the probable cause of the outbreak. compared with previously reported long-term institutional outbreaks of rsv, there were significantly fewer cases of p ... | 1990 | 2358628 |
| productive infection of isolated human alveolar macrophages by respiratory syncytial virus. | respiratory syncytial virus (rsv) is a significant cause of lower respiratory tract disease in children and individuals with cell-mediated immunodeficiencies. airway epithelial cells may be infected with rsv, but it is unknown whether other cells within the lung permit viral replication. we studied whether human alveolar macrophages supported rsv replication in vitro. alveolar macrophages exposed to rsv demonstrated expression of rsv fusion gene, which increased in a time-dependent manner and co ... | 1990 | 2365811 |
| use of serum substitutes for the growth of four cell lines commonly used for virus isolation. | four serum extenders (opti-mem i, biorich, excyte i and excyte iii) and two serum substitutes (omni serum and serum plus) were evaluated for the reduction or replacement of fbs for the growth and maintenance of four representative cell lines used for virus isolation. mrc-5, human fetal foreskin fibroblast (hf), a549 and hep-2 cells were seeded into culture flasks containing mem with 10% fbs or with the serum extenders or substitutes and subcultured into 16 x 125 mm glass tubes and 1-dram shell v ... | 1990 | 2370288 |
| ambisense segment 4 of rice stripe virus: possible evolutionary relationship with phleboviruses and uukuviruses (bunyaviridae). | the complete nucleotide sequence of segment 4 of the rice stripe virus (rsv) genome was determined from overlapping cdna clones and by direct rna sequencing. the segment has two long open reading frames (orfs). one of the orfs (534 bases) is in the 5' region of the viral sequence, and the other (858 bases) is in the viral complementary sequence of the viral 3' region. such ambisense genome organizations have been seen previously in rnas of phleboviruses, uukuviruses and arenaviruses. the orf in ... | 1990 | 2374003 |
| detection of respiratory syncytial virus (rsv) infected cells by in situ hybridization in the lungs of cotton rats immunized with formalin-inactivated virus or purified rsv f and g glycoprotein subunit vaccine and challenged with rsv. | the replication of rsv in unimmunized cotton rats was evaluated by quantitating the amount of infectious virus in the lung and the number of rsv infected cells in a histopathological section of lung by in situ hybridization. rsv infected cells were detected only in alveoli and bronchioles and constituted only a small minority of the cell population. the temporal patterns of rise to the peak number of infected cells (day 4) and the peak titer of infectious virus (day 3) were similar. the clearanc ... | 1990 | 2385958 |
| igg-subclass-specific antibody reactivity to respiratory syncytial virus polypeptides investigated by western blot. | igg1 and igg3 subclass-specific antibody reactivity (abr) in serum samples obtained from infants and children in relation to acute lower respiratory disease caused by respiratory syncytial virus (rsv) infection was investigated by western blot. igg1 abr was directed against the nucleocapsid polypeptides vpn and vpp as well as against the glycoproteins gp48 (f1) and gp90. igg3 abr was directed only against vpn and vpp. in infants, a low igg1 reactivity against glycoproteins was observed. when ser ... | 1990 | 2392617 |
| gene transfer, expression and inheritance of prsv-rainbow trout-gh cdna in the common carp, cyprinus carpio (linnaeus). | a recombinant plasmid containing the rous sarcoma virus-long terminal repeat (rsv-ltr) promoter linked to rainbow trout (salmo gairdneri) growth hormone (gh) cdna was microinjected into fertilized carp eggs. genomic dna extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and southern blot hybridization, using the rsv-ltr and/or the gh cdna sequences as probes. out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain prsv-rtg ... | 1990 | 2393581 |
| [a bronchiolitis epidemic caused by respiratory syncytial viruses]. | in the typical period of the year (autumn-winter 1986-1987) an outbreak of bronchiolitis in infants caused by respiratory syncytial virus (rsv) occurred. the acute course of the illness, a severe clinical picture, and a very fast spread of infection called for the very rapid techniques for detecting the etiological agent. applying the direct immunofluorescent antibody test (dfta) in samples of nasopharyngeal secretions, rsv was found to be responsible for the epidemic. later the same samples of ... | 1990 | 2396494 |
| impact of treatment guidelines on use of ribavirin. | during the 1987 through 1988 seasonal peak of respiratory syncytial virus (rsv), 177 courses of ribavirin were administered at st christopher's hospital for children, a tertiary care medical center in philadelphia, pa. charts were reviewed on 100 treated patients with proved or suspected rsv disease to determine adherence to american academy of pediatrics treatment guidelines. ninety-four percent fulfilled criteria for the risk of significant morbidity: cardiac, pulmonary, or immunodeficiency co ... | 1990 | 2396611 |
| close structural resemblance between putative polymerase of a drosophila transposable genetic element 17.6 and pol gene product of moloney murine leukaemia virus. | we have made a computer-assisted search for homology among polymerases or putative polymerases of various viruses and a transposable element, the drosophila copia-like element 17.6. the search revealed that the putative polymerase (second open reading frame) of the copia-like element 17.6 bears close resemblance in overall structural organization to the pol gene product of moloney murine leukaemia virus (m-mulv): they show significant homology to each other at both the n- and c-terminal portions ... | 1985 | 2408886 |
| inhibition of rous sarcoma virus production by formycin. | the effect of formycin, an adenosine analog, on the growth of chick embryo fibroblasts and on rous sarcoma virus (rsv) production was studied. an adverse effect on cell proliferation was observed in the presence of 10 microm formycin. treatment with 5 microm formycin for 24 hr reduced by a factor of about 1000 the yield of infections progeny whereas the cell growth remained unaltered. moreover the few particles released in the presence of formycin showed a markedly decreased ability to synthesiz ... | 1985 | 2409669 |
| rous sarcoma virus-transformed fibroblasts adhere primarily at discrete protrusions of the ventral membrane called podosomes. | rous sarcoma virus-transformed bhk cells (rsv/b4-bhk) adhere to a fibronectin-coated substratum primarily at specific dot-shaped sites. such sites contain actin and vinculin and represent close contacts with the substratum as revealed by interference reflection microscopy. only a few adhesion plaques and actin filament bundles can be detected in these cells as compared to untransformed parental fibroblasts. in thin sections examined with transmission electron microscopy (tem) these adhesion site ... | 1985 | 2411576 |
| cytotoxic t-cell response to respiratory syncytial virus in mice. | the role of the humoral and cellular arms of the immune response in protection against respiratory syncytial virus (rsv) infection and in the pathogenesis of the severe forms of this disease is poorly understood. the recent demonstration that some inbred mouse strains can be infected with rsv has opened the way to a detailed investigation of rsv immunity. we report here the finding of major histocompatibility complex-restricted, rsv-specific memory cytotoxic t cells in the spleens of balb/c and ... | 1985 | 2411951 |
| neurones express high levels of a structurally modified, activated form of pp60c-src. | neural tissues contain high levels of the cellular homologue of the transforming protein of rous sarcoma virus (rsv), but neither the specific cell types expressing high levels of c-src, nor the function of the cellular src (c-src) protein has been determined. using primary culture methods, we have found that pure neurones and astrocytes derived from the rat central nervous system (cns) contain 15- to 20-times higher levels of the c-src protein than fibroblasts. however, the specific activity of ... | 1985 | 2412121 |
| vinculin and 36 kda protein are not tyrosine-phosphorylated in rous sarcoma virus infected cells which have been treated with interferon. | the expression of membrane-associated transformation-specific parameters was analyzed in de novo rous sarcoma virus (strain sr-rsv-d) infected chicken embryo fibroblasts pretreated with homologous interferon. cellular morphology, hexose transport, microfilament organization, and tyrosine-phosphate content of two primary substrates of the transformation-generating viral kinase, pp60src, were found indistinguishable from non-infected controls. these observations support the hypothesis that vinculi ... | 1985 | 2412817 |
| respiratory syncytial virus infection in transported calves. | nasal swab samples were collected from calves on individual farms in tennessee and sequentially at an auction barn and at a feedlot to detect respiratory syncytial virus (rsv). in 1976, rsv was isolated from 5 of 225 calves at the auction barn and from 13 of 92 calves examined at the feedlot. of the 13 isolations, 11 were from calves with acute respiratory tract disease. most (14/18) calves infected with rsv were also shedding parainfluenza-3 virus in their nasal secretions. attempts to isolate ... | 1985 | 2417510 |
| clinical applications of antiviral agents for chemophrophylaxis and therapy of respiratory viral infections. | table iii summarizes clinical applications of antiviral agents in respiratory viral infections. (table: see text) for influenza a virus infections, both oral amantadine and rimantadine are effective when used for seasonal prophylaxis and for prophylaxis in institutional populations. both of these drugs, as well as aerosolized ribavirin, have antiviral and therapeutic effects in uncomplicated influenza. it remains to be determined whether any of these modalities or possibly their combined use [44 ... | 1985 | 2417551 |
| concurrent circulation of antigenically distinct strains of respiratory syncytial virus during community outbreaks. | respiratory syncytial virus (rsv) is considered to be of a single serotype. antigenic variants are detectable both by neutralization and monoclonal antibodies and have been divided into two broad categories, groups 1 and 2. group 2 isolates have been considered to be uncommon. we used indirect immunofluorescence with strain-specific monoclonal antibodies to study rsv isolates from hospitalized infants in the greater boston area. of 223 rsv isolates recovered over a five-month period in 1983-1984 ... | 1986 | 2418126 |
| the detection of respiratory syncytial virus in nasopharyngeal aspirates: assessment, formulation, and evaluation of monoclonal antibodies as a diagnostic reagent. | comparisons were made between standard methods of cell culture, indirect immunofluorescence (if) using hyperimmune respiratory syncytial virus (rsv) antiserum, and indirect if using mouse monoclonal antibodies directed against various epitopes of rsv for the detection of rsv in nasopharyngeal aspirates. the monoclonal antibodies were used singly and in pools of different specificities which in turn were tested in both direct and indirect if. in a preliminary study, aspirates from 227 infants wer ... | 1986 | 2419492 |
| enhancement of respiratory syncytial virus pulmonary pathology in cotton rats by prior intramuscular inoculation of formalin-inactiva ted virus. | cotton rats previously inoculated with formalin-inactivated respiratory syncytial virus (rsv) were challenged intranasally with live rsv to induce an enhancement of rsv disease similar to that observed after the administration of formalin-inactivated rsv vaccine to human infants 20 years ago. within 24 h after infection with rsv, cotton rats developed pulmonary lesions that reached a maximum by day 4. histologically, the lesions resembled an experimental pulmonary arthus reaction. an action of f ... | 1986 | 2419587 |
| identification of epitopes on respiratory syncytial virus proteins by competitive binding immunoassay. | to characterize the interrelationship of monoclonal antibodies (mabs) against respiratory syncytial virus (rsv) and their respective epitopes, we developed a competitive binding assay based on the biotin-avidin system and a tissue culture enzyme-linked immunosorbent assay. the competitive binding assay clearly distinguished between competing and noncompeting mabs. eight mabs against the fusion protein (f protein) demonstrated two blocking patterns consistent with two antigenic sites. mabs reacti ... | 1986 | 2420819 |
| murine cytotoxic t cells specific to respiratory syncytial virus recognize different antigenic subtypes of the virus. | the aim of this study was to establish whether cytotoxic t cells (tc), raised against respiratory syncytial virus (rsv) in the mouse, are specific to the strain of immunizing virus, or cross-reactive between virus strains. several recent studies using monoclonal antibodies have begun to define the antigenic variation among strains of rsv. it is likely that a successful rsv vaccine will need to contain antigenic determinants from more than one subtype, but since only the highest levels of neutral ... | 1986 | 2420922 |
| polymerase-defective mutant of the bryan high-titer strain of rous sarcoma virus. | a mutant of the bryan high-titer strain of rous sarcoma virus defective in reverse transcriptase is known as type alpha (bh-rsv alpha). bh-rsv alpha virion particles do not contain any polymerase-related proteins but they direct the synthesis of a normal sized pr180 gag-pol polyprotein precursor in infected cells. using a bioassay for polymerase gene function that is based on the requirement of viral replication for transformation of transfected chicken cells, we have localized the defect to the ... | 1986 | 2421248 |
| phosphotyrosine-containing 120,000-dalton protein coimmunoprecipitated with pp60v-src from rous sarcoma virus-transformed mammalian cells. | serum from rabbits bearing tumors (tbr serum) induced by the rous sarcoma virus (rsv) was originally developed to identify the rsv src gene protein-pp60v-src. it is also capable of directly or indirectly immunoprecipitating a number of proteins besides pp60v-src from lysates of rsv-transformed cells. this report describes a highly phosphorylated protein of approximately 120,000 da (pp120) which is specifically immunoprecipitated from rsv-transformed mammalian cells by tbr sera and monospecific a ... | 1986 | 2421483 |
| phosphorylation of tyrosine residues of calmodulin in rous sarcoma virus-transformed cells. | calmodulin, a wide-spread eukaryotic ca2+-binding protein, was phosphorylated at its tyrosine residues in rous sarcoma virus (rsv)-transformed chicken and rat cells but not in normal chicken embryo fibroblasts. in contrast, serine and threonine phosphorylation of calmodulin was found to occur in both normal and virus-transformed cells. in an in vitro system containing purified src kinase from rsv-transformed cells, tyrosine phosphorylation of calmodulin by the src kinase was inhibited by ca2+. f ... | 1986 | 2424020 |
| gene expression from both intronless and intron-containing rous sarcoma virus clones is specifically inhibited by anti-sense rna. | to distinguish the inhibitory effect of anti-sense rna on translation from the effect on splicing, a plasmid (plc32) was constructed from a cdna clone of the rous sarcoma virus (rsv) envelope gene (env) mrna. transcription of this plasmid results in the synthesis of rna identical to the rsv env gene mrna which does not require splicing to be expressed. plasmids derived from plc32 were also constructed in which the env gene coding sequence and 5' noncoding leader sequences were inserted in the op ... | 1985 | 2426579 |
| human and murine cytotoxic t cells specific to respiratory syncytial virus recognize the viral nucleoprotein (n), but not the major glycoprotein (g), expressed by vaccinia virus recombinants. | the viral antigens recognized by cytotoxic t cells (ctl) have not been defined in most viruses infecting mouse or man. natural or artificial virus recombinants can be used to determine the antigen specificity of ctl directed against viruses with segmented genomes, such as influenza, but this technique is more difficult to apply to the study of unsegmented viruses. we describe here the use of recombinant vaccinia viruses, containing cdna corresponding to either the nucleoprotein (n) gene or the m ... | 1986 | 2431051 |
| expression of pp60v-src alters the ionic permeability of the plasma membrane in rat cells. | the transmembrane potential of rous sarcoma virus (rsv)-infected rat-1 cells, expressing the pp60v-src protein kinase, is markedly less negative (by approximately 30 mv) than that of their normal counterparts. by contrast, the membrane potential of rat-1 cells infected with kirsten sarcoma virus is virtually unaltered. the rsv-induced membrane depolarization is shown to be due to a severalfold increase in the cation permeability ratio (pna/pk) of the plasma membrane. when cells infected with a t ... | 1987 | 2434484 |
| detection of phosphotyrosine-containing proteins in polyomavirus middle tumor antigen-transformed cells after treatment with a phosphotyrosine phosphatase inhibitor. | cells transformed with the middle tumor antigen (mt) of polyomavirus were treated with sodium orthovanadate (na3vo4), an inhibitor of phosphotyrosine phosphatases, to enhance for the detection of cellular proteins which are phosphorylated on tyrosine. na3vo4 treatment of mt-transformed rat f1-11 cells resulted in a 16-fold elevation in the level of phosphotyrosine associated with total cellular proteins. parental f1-11 cells displayed only a twofold increase in phosphotyrosine following na3vo4 t ... | 1987 | 2434835 |
| interactions of retroviral structural proteins with single-stranded nucleic acids. | we have studied the interactions of single-stranded polyribonucleotides with murine leukemia virus structural proteins p10, p10' (a p10 variant), and pr65gag, as well as rous sarcoma virus (rsv) pp12 (a p10 analog). two quantitative assays have been used to monitor protein-rna association: the fluorescence enhancement of polyethenoadenylic acid) poly(epsilon a) upon binding protein, and tryptophan fluorescence quenching upon binding to poly(u). with each assay p10 was shown to bind stoichiometri ... | 1987 | 2435721 |
| recognition of rous sarcoma virus-induced tumor antigens by cytotoxic t lymphocytes (ctl): studies on specificity of killing by ctl employing h-2 congenic and recombinant mouse tumor cells. | the specificities of cytotoxic t lymphocytes (ctl) were studied for the analysis of ctl against tumor-specific cell surface antigen(s) (tssa) of non-virus-producing tumor cells induced by the schmidt-ruppin strain of rous sarcoma virus (sr-rsv) in b10 congenic and recombinant mice. eight ctl clones were established from immune spleen cells of b10.a(5r) mice. these clones demonstrated six patterns of cytotoxic reactivity in vitro: two clones showed h-2 restriction in tumor cell lysis. two other c ... | 1987 | 2436809 |
| induction of the ceruloplasmin mrna in balb/c 3t3 cells after rous-sarcoma virus transfection. | ceruloplasmin is a copper containing serum glycoprotein. it is an acute-phase protein and its levels are increased in inflammation and in a number of experimental and human tumors. it is normally synthesized in the liver and not in fibroblasts. in this paper we present evidence that ceruloplasmin mrna is synthesized in balb/c 3t3 cells and its levels are increased about 3-fold in rous-sarcoma virus (rsv) transformed cells. the ceruloplasmin mrnas from rat liver and rsv 3t3 cells have the same el ... | 1987 | 2438032 |
| altered pp60src kinase activity in regressing tumors induced by rous sarcoma virus. | tumors which are induced in chickens by rous sarcoma virus (rsv) usually grow progressively for several weeks and then regress. pp60src kinase activity was found to be reduced in cultured tumor cells which derived from regressing sarcomas by 60-75% as compared to progressively growing neoplasms. we further found that a cellular pp89 which co-precipitated with pp60src was largely diminished in tumor cells derived from regressing as compared with progressively growing sarcomas. in contrast, immuno ... | 1987 | 2438033 |
| histochemical staining of clonal mammalian cell lines expressing e. coli beta galactosidase indicates heterogeneous expression of the bacterial gene. | an evaluation has been made of the e. coli beta-galactosidase (beta-gal) gene for use as a reporter gene in mammalian cells in culture. we have adopted a histochemical procedure which enables identification of those cells within a population that express the introduced bacterial gene. data is presented concerning the sensitivity of the histochemical method relative to an immunological method of detection. it has been found that several clonal cell lines generated after transfection of human 293 ... | 1987 | 2440117 |
| modulation of monocyte mo2 surface antigen expression by exposure to respiratory syncytial virus. | the mo2 antibody recognizes a monocyte-specific cell surface antigen of unknown function. upward modulation of mo2 surface epitope density was demonstrated in response to 72-hr culture of monocytes with respiratory syncytial virus (rsv), but this was not seen after culture with phytohemagglutinin or other respiratory viruses. monoclonal antibody probes for rsv proteins were used to demonstrate probable replication of rsv proteins in peripheral blood monocytes and pulmonary alveolar macrophages. ... | 1987 | 2440300 |
| monoclonal antibodies to the large glycoproteins of respiratory syncytial virus: possible evidence for several functional antigenic sites. | employing different routes of immunization and different adjuvants, a large number of hybridomas were produced that secreted monoclonal antibodies (mabs) against the large envelope glycoprotein (gp90) and the fusion glycoprotein (gp70) of respiratory syncytial virus (rsv). the antigenic specificity of the mabs was established by immunoblot analysis and radioimmunoprecipitation. three hybridomas secreting anti-gp90 mabs were established from mice immunized by intraperitoneal injection of purified ... | 1987 | 2440982 |
| the g glycoprotein of human respiratory syncytial viruses of subgroups a and b: extensive sequence divergence between antigenically related proteins. | two major antigenic subgroups (designated a and b) have been described for human respiratory syncytial virus (rsv). previously, on the basis of reactivity patterns with monoclonal antibodies, the greatest intersubgroup variation was shown to occur in the g protein, the putative attachment glycoprotein. to delineate the molecular basis for this variation, we have determined the nucleotide and deduced amino acid sequences of the g mrnas and proteins representing a subgroup a (long strain) and a su ... | 1987 | 2441388 |
| antiviral chemotherapy and prophylaxis of viral respiratory disease. | respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ... | 1987 | 2446820 |
| characterization of ribosomal frameshifting in hiv-1 gag-pol expression. | based on precedents from other retroviruses, the precursor of the human immunodeficiency virus (hiv-1) reverse transcriptase is predicted to be a polyprotein with a relative molecular mass (mr) of 160,000 (160k) encoded by both the viral pol gene and the upstream gag gene. these two genes lie in different translational reading frames, with the 3' end of gag overlapping the 5' end of pol by 205 or 241 nucleotides. thus, production of the gag-pol fusion protein would require either messenger rna p ... | 1988 | 2447506 |
| nonmyristoylated p60v-src fails to phosphorylate proteins of 115-120 kda in chicken embryo fibroblasts. | we have used anti-phosphotyrosine antibodies to identify a large number of tyrosine phosphoproteins in rous sarcoma virus (rsv)-transformed chicken embryo fibroblasts. most of these proteins were found in the 100,000 x g supernatant when cells were separated into soluble and particulate fractions; however, one group of proteins, of 115-120 kda, was found in the particulate fraction. the phosphotyrosine content of the 115- to 120-kda proteins was greatly reduced in chicken embryo fibroblasts infe ... | 1988 | 2451829 |
| identification of multiple novel polypeptide substrates of the v-src, v-yes, v-fps, v-ros, and v-erb-b oncogenic tyrosine protein kinases utilizing antisera against phosphotyrosine. | antibodies to phosphotyrosine were used to identify proteins phosphorylated on tyrosine in chicken embryo fibroblasts transformed by the oncogenes, v-src, v-yes, v-fps, v-ros, or v-erb-b. these antibodies allowed detection of a minimum of 50, 44, and 47 bands in immunoblots of cellular lysates from fibroblasts transformed by v-src, v-yes, or v-fps respectively. eight of these bands were also detected in fibroblasts transformed by v-ros and v-erb-b, suggesting that the cellular transformation ind ... | 1988 | 2452398 |
| separation of the tumor rejection antigen of rous sarcoma virus-induced murine fibrosarcoma. | the tumor antigen capable of inducing tumor resistance (tumor rejection antigen; tra) was separated and some of its physicochemical properties were characterized. cytosol and plasma membrane fractions were separated from rous sarcoma virus (rsv)-induced csa1m tumor cells. immunization with membrane but not cytosol fraction of these tumor cells together with complete freund's adjuvant resulted in complete protection against subsequent challenge with viable csa1m cells. the tra activity contained ... | 1988 | 2453498 |
| use of an epstein-barr virus episomal replicon for anti-sense rna-mediated gene inhibition in a human cytotoxic t-cell clone. | a methodology was developed for stable gene transfer into cloned nontransformed human t lymphocytes. stable high-level gene expression was achieved in cloned human t cells by using a self-replicating epstein-barr virus (ebv) episomal replicon. a comparison of five eukaryotic promoters established that the rous sarcoma virus 3' long terminal repeat (rsv 3' ltr) and the lymphopapilloma virus (lpv) 5' ltr are optimal for episome-based expression in t cells. effective (greater than 95%), selective, ... | 1988 | 2453884 |
| enforced expression of the c-myc oncogene inhibits cell differentiation by precluding entry into a distinct predifferentiation state in g0/g1. | a broad base of data has implicated a role for the c-myc proto-oncogene in the control of the cell cycle and cell differentiation. to further define the role of myc in these processes, i examined the effect of enforced myc expression on several events that are thought to be important steps leading to the terminally differentiated state: (i) the ability to arrest growth in g0/g1, (ii) the ability to replicate the genome upon initiation of the differentiation program, and (iii) the ability to lose ... | 1988 | 2454393 |
| an enzyme-linked immunosorbent assay using monoclonal antibodies for the detection of respiratory syncytial virus in clinical specimens. | an enzyme-linked immunosorbent assay (elisa) has been developed for the detection of respiratory syncytial virus in nasopharyngeal secretions. this assay employed as immunoreagents two monoclonal antibodies directed against two distinct epitopes of the viral nucleocapsid. one of them (rsv 4) was used for antigen capture and the other (nc 4) was labelled with n-hydroxy-succinimide-epsilon-caproil biotin and used for antigen detection. streptavidin biotin-peroxidase complexes were employed as ampl ... | 1988 | 2455493 |
| immunological characterization of proteins detected by phosphotyrosine antibodies in cells transformed by rous sarcoma virus. | phosphotyrosine antibodies were used to identify tyrosine-phosphorylated proteins in rous sarcoma virus (rsv)-transformed chicken embryo fibroblasts. a large number of tyrosine phosphoproteins were detected. a similar set of proteins was observed in rsv-transformed murine cells. an 85,000-dalton protein, however, was present in transformed avian cells but missing in transformed murine cells. neither the 85,000-dalton protein nor any of the other tyrosine phosphoproteins appeared to be viral stru ... | 1988 | 2455815 |
| vanadate-treated baby hamster kidney fibroblasts show cytoskeleton and adhesion patterns similar to their rous sarcoma virus-transformed counterparts. | rous sarcoma virus-transformed baby hamster kidney fibroblasts (rsv/b4-bhk) adhere to a fibronectin-coated substratum by means of dot-like adhesion sites called podosomes in view of their shape and function as cellular feet (tarone et al.: exp cell res 159:141, 1985). podosomes concentrate tyrosine-phosphorylated proteins, including pp60v-src, and appear in many cells transformed by oncogenes coding for tyrosine kinases. in this paper we used orthovanadate, an inhibitor of phosphotyrosine phosph ... | 1988 | 2456294 |
| tonsillitis and respiratory infections of viral aetiology in a developing country. | among 75 clinically diagnosed cases of tonsillitis, 55 (73.3%) were found to be of viral aetiology. serological and virological investigations identified adenovirus as the main causative agent in children as well as in adults. in only two instances, both in children, was an association with respiratory syncytial virus (rsv) in tonsillitis detected, an extremely rare finding. a cross-sectional sero-epidemiological study of respiratory infections caused by adenovirus, rsv, influenza (a,b) and para ... | 1988 | 2456726 |
| rapid titration of bovine, caprine and human rs virus by a micro-immunoperoxidase assay using a monoclonal antibody and a permissive ovine kidney cell line. | an indirect immunoperoxidase micro-assay, using a continuous cell line derived from ovine kidney cells (ok) and a previously characterized monoclonal antibody (7c2), specific for an exposed and highly conserved epitope of the fusion protein of different strains of rs virus, was used advantageously to rapidly titrate bovine, caprine and human strains of rsv by either quantal (tcid50) or plaque forming assays. virus titers, obtained in less than 36 h, were in agreement with those obtained by the c ... | 1988 | 2458375 |
| an immunoperoxidase method of detecting respiratory syncytial virus antigens in paraffin sections of pneumonic bovine lung. | using an avidin-biotin-peroxidase complex immunoperoxidase staining method, respiratory syncytial virus (rsv) antigen was demonstrated in glutaraldehyde-fixed, paraffin-processed lung sections from calves with induced rsv pneumonia. the virus also was detected in formalin-fixed, paraffin-processed lung sections from calves with naturally occurring rsv pneumonia. specific immunoperoxidase staining was detected within the cytoplasm of epithelial cells and syncytia in small bronchi, bronchioli, and ... | 1988 | 2458691 |
| characteristics of in vitro production of mucosal antibody to respiratory syncytial virus in tonsillar tissue lymphocytes. | tonsillar lymphocytes (tl) obtained from 39 subjects were established in culture in vitro, and analyzed for respiratory syncytial virus (rsv) specific antibody synthesis after stimulation with pokeweed mitogen and live or ultraviolet light inactivated rsv. following stimulation with the virus, igg1, iga, and low levels of igg3 but no igg2, igg4, or ige anti-rsv antibody could be detected in the culture supernatants. the anti-rsv antibody production in tl did not show any correlation with the pre ... | 1988 | 2458875 |
| small finger protein of avian and murine retroviruses has nucleic acid annealing activity and positions the replication primer trna onto genomic rna. | retrovirus virions carry a diploid genome associated with a large number of small viral finger protein molecules which are required for encapsidation. our present results show that finger protein p12 of rous sarcoma virus (rsv) and p10 of murine leukaemia virus (mulv) positions replication primer trna on the replication initiation site (pbs) at the 5' end of the rna genome. an rsv mutant with a val-pro insertion in the finger motif of p12 is able to partially encapsidate genomic rna but is not i ... | 1988 | 2458920 |
| formalin-inactivated respiratory syncytial virus vaccine induces antibodies to the fusion glycoprotein that are deficient in fusion-inhibiting activity. | the fusion (f) glycoprotein of respiratory syncytial virus (rsv) induces neutralizing antibodies and antibodies that inhibit fusion of infected cells (fi antibody). it was previously shown that infants and children immunized with formalin-inactivated rsv 20 years ago developed antibodies that bound to the f glycoprotein but were deficient in neutralizing activity. a reexamination of these sera indicated that they were also deficient in fi activity. thus, formalin-inactivated rsv vaccine stimulat ... | 1988 | 2459154 |
| proposed gag-encoded transcriptional activator is not necessary for rous sarcoma virus replication or transformation. | it has been reported that gene expression directed by the long terminal repeat of rous sarcoma virus (rsv) is trans activated by a protein encoded in an alternate reading frame within the rsv gag gene (s. broome and w. gilbert, cell 40:537-546, 1985). we have made specific mutations to test the role of the putative transcriptional activator in rsv replication. termination codons were created within the alternate reading frame coding for the trans activator, and the mutations were introduced into ... | 1988 | 2459410 |
| neutralization of respiratory syncytial virus by individual and mixtures of f and g protein monoclonal antibodies. | we identified several types of neutralization effected by f and g protein monoclonal antibodies (mabs) reacted individually or as mixtures against respiratory syncytial virus (rsv). neutralizing activity was identified by a microneutralization test in which virus replication was determined by enzyme immunoassay. complete neutralization was seen only with mabs against the f protein. strain-specific neutralization, complete neutralization against some strains of rsv, and no neutralization against ... | 1988 | 2459412 |
| transduction of proto-src sequences in tissue culture by a molecular clone of transformation-defective rous sarcoma virus with an internal src deletion. | the sporadic appearance of nondefective (nd) rous sarcoma virus (rsv) from cells in tissue culture infected with a molecular clone of transformation-defective rsv was examined. southern analysis of extrachromosomal, virus-specific dna of three independent ndrsv isolates in each case indicated restoration of an isogenic src by homologous recombination with cellular proto-src. the frequency of transduction was estimated by fluctuation analysis to vary between one transduction per 0.4 x 10(7) to 1. ... | 1988 | 2460643 |
| a mutation in v-src that removes a single conserved residue in the sh-2 domain of pp60v-src restricts transformation in a host-dependent manner. | the v-src oncogene of rous sarcoma virus (rsv) is able to transform both avian and mammalian cells, but the mutant allele v-src-l displays a host range dependence for transformation, transforming chicken but not rat cells with wild-type efficiency. this host range restriction can be detected by measuring growth in low serum, saturation density, and anchorage independent growth. in addition, rat cells expressing v-src-l do not form tumors in syngeneic rats or nude mice, but rsv carrying the mutan ... | 1989 | 2462061 |
| pyruvate kinase type m2 is phosphorylated at tyrosine residues in cells transformed by rous sarcoma virus. | chicken embryo cells (cecs) contain pyruvate kinase (pk) type m2 (m2-pk). transformation of cecs by rous sarcoma virus (rsv) leads to a reduction in the affinity of pk for the substrate phosphoenolpyruvate. in vitro, m2-pk can be phosphorylated at tyrosine residues by pp60v-src, the transforming protein of rsv. to study tyrosine phosphorylation of m2-pk in intact rsv-transformed cells, the protein was immunoprecipitated from 32p-labeled normal and rsv-sr-a-transformed cecs. phosphoamino acid ana ... | 1988 | 2462512 |
| the ltr, v-src, ltr provirus generated in the mammalian genome by src mrna reverse transcription and integration. | different types of altered proviruses of rous sarcoma virus (rsv) have been detected in mammalian tumor cell lines. we cloned and sequenced one of these altered proviruses with the structure ltr, v-src, ltr. the presence of an intact viral splice junction, as well as duplications of the chromosomal sequence gcgggg flanking the two 2-base-pair-deleted ltrs, demonstrated reverse transcription and normal retroviral integration of src mrna in mammalian cells. in addition, a 1-nucleotide deletion 2 b ... | 1989 | 2463374 |
| mechanism of reversion of rous sarcoma virus transformation by herbimycin a: reduction of total phosphotyrosine levels due to reduced kinase activity and increased turnover of p60v-src1. | we studied the mechanism of reversion of rous sarcoma virus (rsv)-transformation by herbimycin a in a temperature-sensitive rsv-infected nrk cell line. herbimycin reduced the total cellular phosphotyrosine level to 10% of the control value. intracellular p60src kinase activity was reduced significantly within 3 h of herbimycin treatment, but recovered to approximately 50% of the control within 24 h, and the kinetics paralleled the decrease of the phosphorylation of a cellular target p36. partial ... | 1989 | 2463877 |
| synergistic killing of virus-transformed human cells with interferon and n-methyl-n'-nitro-n-nitrosoguanidine. | interferons potentiate the cytotoxic effects of certain antineoplastic drugs on human tumor cells both in vitro and in vivo, although the mechanism of interferon's synergistic action is unknown. interferon may act by modulating the expression of dna repair activity in cells. to test this hypothesis, we maintained parallel cultures of normal o6-methylguanine repair-proficient human fibroblasts and tumor cells, or rsv-and sv40-transformed repair-deficient mer- human fibroblasts in medium containin ... | 1989 | 2463881 |
| most of the substrates of oncogenic viral tyrosine protein kinases can be phosphorylated by cellular tyrosine protein kinases in normal cells. | the cellular transformation induced by viral tyrosine protein kinases may result from the excessive phosphorylation of the normal polypeptide substrates of endogenous cellular tyrosine kinases, from the phosphorylation of proteins that are not normal substrates of cellular tyrosine protein kinases in uninfected cells, or from the phosphorylation of proteins of each type. to differentiate between these possibilities, antibodies to phosphotyrosine were used with immunoblotting to compare the subst ... | 1988 | 2465525 |
| antigenic variation of human rsv strains isolated in japan. | antigenic variations of respiratory syncytial virus (rsv) strains were analyzed using a collection of nine, seven, two, and one monoclonal antibodies (mabs), respectively, raised against the fusion protein (f), large glycoprotein (g), nucleoprotein (np), and phosphoprotein (p) components of the long strain of rsv. competitive binding assay by these mabs demonstrated eight, four, and two distinct epitopes on f, g, and np components, respectively. comparison of prototype long with ten field strain ... | 1989 | 2466102 |
| integrins isolated from rous sarcoma virus-transformed chicken embryo fibroblasts. | avian integrins are a complex of three integral membrane glycoproteins that are thought to have a role both in anchoring the cytoskeleton to the plasma membrane and establishing linkages to the extracellular matrix. we previously demonstrated that bands 2 and 3 of integrin are phosphorylated on both tyrosine and serine residues in chicken embryo fibroblasts (cef) transformed with rous sarcoma virus (rsv) and other oncogenic retroviruses. the effects of rsv transformation on integrins from chick ... | 1989 | 2468126 |
| [biochemical characterization of tumor rejection antigen (tra) and genetic control in anti-tra immune responses]. | a tumor antigen capable of inducing tumor resistance (tumor rejection antigen; tra) was obtained in a solubilized form by sodium dodecyl sulfate (sds)-extraction of plasma membrane fraction from rous sarcoma virus (rsv)-induced csa1m fibrosarcoma cells (balb/c origin). analyses by sephacryl s-300 gel filtration and sds-polyacrylamide gel electrophoresis revealed that tra activity was recovered in the fraction of an approximate molecular weight (m. w.) of 60kd. such tra was also found to exist in ... | 1989 | 2468311 |
| neutralization epitopes of the f glycoprotein of respiratory syncytial virus: effect of mutation upon fusion function. | eighteen neutralizing monoclonal antibodies (mabs) specific for the fusion glycoprotein of the a2 strain of respiratory syncytial virus (rsv) were used to construct a detailed topological and operational map of epitopes involved in neutralization and fusion. competitive binding assays identified three nonoverlapping antigenic sites (a, b, and c) and one bridge site (ab). thirteen mab-resistant mutants (marms) were selected, and the neutralization patterns of the mabs with either marms or rsv cli ... | 1989 | 2470922 |
| recognition of respiratory syncytial virus fusion protein by mouse cytotoxic t cell clones and a human cytotoxic t cell line. | two mouse cytotoxic t cell (tc) clones, d5 and h11a, with specificity for the respiratory syncytial virus (rsv) fusion protein (f) were derived from balb/c mice primed intranasally (i.n.) with rsv (a2 strain). these clones possessed essentially the same characteristics, and only clone h11a is described here. tc clone h11a lysed target cells infected with a recombinant vaccinia virus (vv) expressing the rsv f gene, and similar target cells infected with rsv strains long, 8/60, or 18537. in additi ... | 1989 | 2471784 |
| novel tyrosine kinase substrates from rous sarcoma virus-transformed cells are present in the membrane skeleton. | we have previously reported the production of monoclonal antibodies directed against phosphotyrosine, which is the modification induced by many oncogene products and growth factor receptors. one of these antiphosphotyrosine antibodies (py20) was used in affinity chromatography to isolate phosphotyrosine (py)-containing proteins from rous sarcoma virus-transformed chick embryo fibroblasts (rsv-cefs). mice were immunized with these py-proteins for the production of monoclonal antibodies to individ ... | 1989 | 2472406 |
| [transcription and expression in escherichia coli of src gene cloned sequences of rous sarcoma virus]. | effective transcription of virus-specific sequences was shown in escherichia coli cells that carry recombinant plasmids pprc11 and psrcc with fragments of the rous sarcoma virus (rsv) genome. analysis of transcripts revealed several classes of rna, one of which is probably transcribed from the structural part of the rsv src gene. analysis of the primary structure of the region adjacent to the src rsv gene showed the existence of sequences similar to the bacterial promoters from which the src-spe ... | 1989 | 2472549 |
| evidence that a phosphotyrosine-containing 120,000 da protein from rous sarcoma virus-infected cells is phosphorylated by pp60v-src. | phosphorylation of a phosphotyrosine-containing 120,000 da protein (pp120) in rous sarcoma virus (rsv)-infected mammalian cells and in in vitro protein kinase reactions was examined. phosphorylated pp120 was co-immunoprecipitated with anti-pp60v-src antibodies only from rsv-transformed or revertant vole fibroblasts which contained active pp60v-src tyrosine kinase activity or from temperature-sensitive rsv-infected vole cells grown at the permissive temperature. pp120 was phosphorylated on tyrosi ... | 1989 | 2472591 |
| two interferon sensitive steps in the replication cycle of rous sarcoma virus. | de novo infection of rous sarcoma virus (rsv) strains of receptor subgroups a, b, c, and d is inhibited by low doses of chick interferon. adsorption and penetration into the cell are not marginally impaired by interferon treatment. since the level of proviral dna synthesis is strongly reduced in the interferon-treated cell it is concluded that uncoating or reverse transcription of the viral genome is inhibited. this inhibition of proviral dna synthesis is not caused by an arrest of cef in the st ... | 1989 | 2474893 |
| respiratory syncytial virus infection of human cord and adult blood monocytes and alveolar macrophages. | we studied the permissiveness of human leukocytes, blood monocytes, alveolar macrophages, and cord blood monocytes to infection with respiratory syncytial virus (rsv). specific immunofluorescence was used to determine the percentage of infected leukocytes. the results indicated that monocytes were the most susceptible human leukocyte to in vitro infection with rsv. polymorphonuclear leukocytes demonstrated no specific fluorescent staining after 24 h of exposure to rsv, whereas peripheral blood n ... | 1989 | 2476959 |
| hiv promoter activity in primary antigen-specific human t lymphocytes. | human retroviruses, such as the hiv, infects human t cells, and efficient hiv replication occurs primarily in activated t cells rather than resting cells. increased hiv production is likely caused by the activation of the retroviral promoter, and the hiv promoter may be regulated by intracellular signals induced during immune stimulation. to examine the regulation of retroviral promoter activity in normal, ag-specific primary t lymphoblasts, a heterogeneous population of primary human t cells wa ... | 1989 | 2477457 |
| characterization of a novel human respiratory syncytial virus chimeric fg glycoprotein expressed using a baculovirus vector. | human respiratory syncytial virus (rsv) codes for two glycoproteins (f and g) which have been shown to the major targets for the host antibody response. we have expressed a novel chimeric glycoprotein (fg) in insect cells using a baculovirus vector. the chimeric glycoprotein contains the signal and extracellular regions of the rsv f glycoprotein linked to the extracellular region of the rsv g glycoprotein. beginning at the amino terminus, the chimeric glycoprotein consists of amino acids 1 to 48 ... | 1989 | 2477497 |
| biological and biochemical properties of the c-src+ gene product overexpressed in chicken embryo fibroblasts. | the c-src protein isolated from neuronal cells (pp60c-src+) displays a higher level of protein kinase activity than does pp60c-src from nonneural tissues. there are two structural alterations present in the amino-terminal half of pp60c-src+ expressed in neurons which could contribute to the enhanced activity of this form of pp60c-src: (i) a hexapeptide insert located at amino acid 114 of avian pp60c-src+ and (ii) a novel site(s) of serine phosphorylation. we characterized pp60c-src+ expressed in ... | 1989 | 2477684 |
| comparison of antigenic sites of subtype-specific respiratory syncytial virus attachment proteins. | a panel of 19 monoclonal antibodies (mabs) were used to probe the antigenic relationships between the g (attachment) proteins of a and b respiratory syncytial virus (rsv) subtypes (ga and gb). at least three and two antigenic sites were present on ga and gb, respectively, including a shared neutralizing site. most of the antibodies had some degree of complement-independent neutralizing capacity, but in common was a large neutralization-resistant fraction of virus (range 13 to 78%). passive admin ... | 1989 | 2479715 |
| [a stat-method of determining respiratory syncytial virus]. | for rapid detection of rs virus we have modified agglutination test with staphylococcus coated with rsv-antibody which allows the virus titer to be determined within 3-5 min. the results of rs virus titration in the yield compared with those obtained by cft and cpe tests showed our modified test to be twice as specific and sensitive (60-80 ng/ml). this modification of the coagglutination test with sensitized staphylococcus and the method of running the test on a row of slides may be used in viro ... | 1989 | 2480026 |
| early identification and retrieval or deletion of human lymphocyte subpopulations responding to influenza virus or respiratory syncytial virus challenge. | differences in immune responses of human mononuclear leukocytes (mnl) have been demonstrated following exposure in vitro to influenza virus or respiratory syncytial virus (rsv). in the current studies, we sought to identify early differences in reactive subpopulations that emerge from within the heterogeneous resting mnl pool after challenge. mnl were sham-exposed or exposed to influenza virus or rsv, separated, and retrieved by countercurrent centrifugal elutriation after 3 d. exposure to influ ... | 1989 | 2480179 |
| the tumor rejection antigen separated from rous sarcoma virus-induced murine fibrosarcoma exhibits a molecular weight of approximately 60 kd but differs from functional pp60src. | the tumor antigen capable of inducing tumor resistance (tumor rejection antigen; tra) was obtained in a solubilized form by sodium dodecyl sulfate (sds) extraction of plasma membrane fraction from rous sarcoma virus (rsv)-induced csa1m fibrosarcoma cells (balb/c origin). analyses by sephacryl s-300 gel filtration and sds-polyacrylamide gel electrophoresis revealed that tra activity was recovered in the fraction with a molecular weight of approximately 60 kd. unfractionated crude sds-solubilized ... | 1989 | 2480944 |
| ultrastructure and gold-immunolabelling of cell-substratum adhesions (podosomes) in rsv-transformed bhk cells. | rous sarcoma virus-transformed bhk (rsv/b4-bhk) cells develop peculiar dot-like adhesions, that have been named podosomes, which, in the presence of serum, aggregate into ring- or crescent-shaped adhesion sites, the rosettes of podosomes. we have used the lysis-squirting technique and gold-immunolabelling to study the 3d-organisation of podosomes and the location of vinculin, gelsolin, phosphotyrosine-containing proteins and pp60src at an ultrastructural level. podosomes appear to be conical bod ... | 1989 | 2482298 |
| major histocompatibility complex class ii (ia-like) antigens on chicken embryo fibroblasts: effect of transformation by rous sarcoma virus. | in this study, three monomorphic monoclonal antibodies to chicken mhc class ii molecules (b-l) were tested for reactivity in normal and rsv-transformed embryo fibroblasts. the immunocytochemical staining, the cell-bound elisa assay, and the immunoprecipitation analysis showed that all three antibodies reacted with the b-l (ia-like) molecules on normal cells of different genotypes. conversely, the expression of these antigens was not detected in fibroblasts cultured from feather follicles of adul ... | 1989 | 2483835 |