Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [dimers of the nuclear polyhedrosis virus of b. mori]. | 1971 | 5137546 | |
| observations on the nature of the carbonate dissolution process on inclusion bodies of a nuclear polyhedrosis virus of pseudaletia unipuncta. | 1971 | 5151910 | |
| [electron microscopic study of the effect of subcritical temperatures on the dna of the nuclear polyhedrosis virus of bombyx mori]. | 1971 | 5153988 | |
| activation of latent nuclear polyhedrosis virus under the influence of tipula iridescent virus. | 1966 | 5225092 | |
| biological activity of dna preparations isolated from the tissues of silkworms infected with nuclear polyhedrosis virus. | 1966 | 5225093 | |
| cell proliferations in lymantria dispar l. larvae infected with the nuclear polyhedrosis virus. | 1969 | 5348548 | |
| [purification of the polyhedral protein of nuclear polyhedrosis virus of mulberry silkworm and association of the protein in solution]. | 1969 | 5358498 | |
| [electron microscopic investigation of dna isolated from nuclear polyhedrosis virus of bombyx mori]. | 1969 | 5361983 | |
| biochemical studies on the multiplication of a nuclear-polyhedrosis virus in the silkworm, bombyx mori. ii. protein synthesis in larval tissues after infection. | 1969 | 5369195 | |
| biochemical studies on the multiplication of a nuclear-polyhedrosis virus in the silkworm, bombyx mori. 3. functional changes in infected cells, with reference to the synthesis of nucleic acids and proteins. | 1969 | 5369196 | |
| biochemical studies on the multiplication of a nuclear-polyhedrosis virus in the silkworm, bombyx mori. i. nucleic-acid synthesis in larval tissues after infection. | 1969 | 5385135 | |
| electron-microscopic studies on hemocytes of the egyptian cottonworm, spodoptera littoralis (boisduval) infected with a nuclear-polyhedrosis virus, as compared to noninfected hemocytes. i. noninfected hemocytes. | 1969 | 5385137 | |
| inhalation and dermal allergenicity studies of a nuclear-polyhedrosis virus of heliothis zea in guinea pigs. | 1970 | 5435794 | |
| toxicity-pathogenicity studies of a nuclear-polyhedrosis virus of heliothis zea in white mice. | 1970 | 5442844 | |
| long-term feeding and other toxicity-pathogenicity studies on rats using a commercial preparation of the nuclear-polyhedrosis virus of heliothis zea. | 1970 | 5449736 | |
| immunoelectrophoresis of hemolymph of the cabbage looper, trichopusia ni, during the course of a nuclear polyhedrosis virus infection. | 1970 | 5449741 | |
| recovery of the nuclear polyhedrosis virus of the cabbage looper, trichoplusia ni, by coprecipitation with lactose. | 1970 | 5449744 | |
| replication of a nuclear polyhedrosis virus in an established insect cell line. | 1970 | 5482779 | |
| [adaptation of nuclear polyhedrosis virus of bombyx mori l. to galleria mellonella (l.)]. | 1970 | 5503678 | |
| [the variability in form of polyhedra in insect tissue cultures infected with nuclear polyhedrosis virus]. | 1970 | 5517369 | |
| [multiplication of nuclear polyhedrosis virus, activated by cooling, in bombyx mori caterpillers]. | 1970 | 5517382 | |
| [electron microscopic study of the structure of nuclear polyhedrosis virus of bombyx mori]. | 1967 | 5618110 | |
| [isolation and physical chemical properties of the dna of the silkworm nuclear-polyhedrosis virus]. | 1967 | 5623629 | |
| on cross infectivity of the cabbage-looper nuclear-polyhedrosis virus to some related plusinae (noctuidae: lepidoptera). | 1968 | 5649246 | |
| sodium hypochlorite and formalin as antiviral agents against nuclear-polyhedrosis virus in larvae of the cabbage looper. | 1968 | 5649248 | |
| the inactivation of the nuclear polyhedrosis virus of trichoplusia ni by gamma and ultraviolet radiation. | 1968 | 5681529 | |
| studies on dna synthesis in silkworm pupae infected with nuclear polyhedrosis virus. | 1968 | 5709265 | |
| abnormal cell proliferation in the epidermis of the fall webworm, hyphantria cunea, induced by the infection of a nuclear-polyhedrosis virus. | 1968 | 5716587 | |
| dosage-mortality data on a nuclear-polyhedrosis virus of the bollworm, heliothis zea. | 1968 | 5716589 | |
| the fine structure of a nuclear-polyhedrosis virus of the silkworm. | 1968 | 5725009 | |
| the effect of human gastric juice on the pathogenicity of heliothis zea nuclear-polyhedrosis virus. | 1968 | 5758639 | |
| a study of the development of a nuclear-polyhedrosis virus of heliothis in galleria mellonella larvae exposed to gamma rays. | 1969 | 5804150 | |
| a nuclear-polyhedrosis virus of anthela varia (lepidoptera: anthelidae). | 1969 | 5804151 | |
| the nuclear-polyhedrosis virus of heliothis zea (boddie) and heliothis virescens (fabricius). v. toxicity-pathogenicity of virus to white mice and guinea pigs. | 1965 | 5829858 | |
| the nucleic acid of nuclear-polyhedrosis virus of the silkworm. | 1965 | 5867034 | |
| [biological activity of dna preparations isolated from silkworm tissues infected with nuclear polyhedrosis virus]. | 1965 | 5869043 | |
| [activation of latent nuclear polyhedrosis virus under the effect of tipula iridescent virus]. | 1965 | 5869044 | |
| [investigation of serological interrelations between polyhedron protein and nuclear polyhedrosis virus of some lepidoptera]. | 1965 | 5869045 | |
| rna changes in insect tissue infected with a nuclear-polyhedrosis virus. | 1966 | 5905535 | |
| exposure of white mice and guinea pigs to the nuclear-polyhedrosis virus of the cabbage looper, trichoplusia ni. | 1966 | 5905543 | |
| effects of age on mortality of heliothis zera and heliothis virescens larvae exposed to a nuclear-polyhedrosis virus. | 1966 | 5939398 | |
| protection of a susceptible insect host against a nuclear-polyhedrosis virus by ether extracts from insect larvae. | 1966 | 5939399 | |
| effects of temperature on mortality of heliothis zea larvae exposed to sublethal doses of a nuclear-polyhedrosis virus. | 1966 | 5939401 | |
| integration of the heliothis nuclear polyhedrosis virus into a biological control program on cotton. | 1966 | 5976101 | |
| [isolation of pure infectious particles of nuclear polyhedrosis virus of the silkworm]. | 1966 | 5986877 | |
| bioassay of nuclear-polyhedrosis virus against larval instars of the gypsy moth. | 1967 | 6064155 | |
| electron-microscope investigation of the structure of the nuclear-polyhedrosis virus of the silkworm, bombyx mori. | 1967 | 6064158 | |
| human feeding tests using a nuclear-polyhedrosis virus of heliothis zea. | 1967 | 6073679 | |
| transcription of overlapping sets of rnas from the genome of autographa californica nuclear polyhedrosis virus: a novel method for mapping rnas. | the insect baculovirus autographa californica nuclear polyhedrosis virus (acnpv) contains a double-stranded, supercoiled circular genome of 126 to 129 kilobase pairs in length. in cultured spodoptera frugiperda insect cells the virus replications, and early and late phases of viral genome expression are discernible. we previously mapped 5 early and at least 32 late different viral polypeptides on the viral genome (h. esche, h. lübbert, b. siegmann, and w. doerfler, embo j. 1:1629-1633, 1982). ho ... | 1984 | 6090702 |
| the antiviral spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine. | the antiviral activity spectrum of (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) is not restricted to herpes simplex virus type 1 (hsv-1) and varicella-zoster virus (vzv) but also encompasses several other herpesviruses such as suid herpesvirus type 1 (shv-1), bovid herpesvirus type 1 (bhv-1), simian varicella virus (svv), herpesvirus saimiri, herpesvirus platyrrhinae, and the baculovirus trichoplusia ni multiple nuclear polyhedrosis virus. other herpesviruses such as herpes simplex virus type 2, ... | 1984 | 6092320 |
| conservation of genome organization in two multicapsid nuclear polyhedrosis viruses. | the genome of the multicapsid nuclear polyhedrosis virus of orgyia pseudotsugata was mapped by examining overlapping hindiii fragments from cosmid clones which had been constructed from partial hindiii digests of viral dna. five opmnpv cosmid clones containing fragments encompassing the entire opmnpv genome were hydridized to blots of dna from the multicapsid nuclear polyhedrosis virus of autographa californica. the hybridization pattern indicated that the genomes of these viruses are similarly ... | 1984 | 6092694 |
| phenotypic characterization and physical mapping of a temperature-sensitive mutant of autographa californica nuclear polyhedrosis virus defective in dna synthesis. | a ts mutant of autographa californica nuclear polyhedrosis virus (acmnpv), ts8, was shown to be defective in viral dna synthesis at the nonpermissive temperature. ts8-infected cells synthesized only early viral polypeptides at the nonpermissive temperature, and in contrast to wild-type (wt)-infected cells, showed no inhibition of host cell protein synthesis. the effect of the mutation on viral dna synthesis was not immediately reversed after shifting infected cells down from the nonpermissive te ... | 1984 | 6093362 |
| chemically-defined media for production of insect cells and viruses in vitro. | two chemically-defined media are described. they support the growth of a) an established cell line of spodoptera frugiperda cells and b) two established mosquito cell lines from aedes aegypti and anopheles gambiae. the replication of autographa californica nuclear polyhedrosis virus (acnpv) in s. frugiperda cells grown in a defined medium is reported. | 1980 | 6102531 |
| the process of virus assembly in insect virus mixed infections. | interference occurred upon infection of the cabbage moth caterpillars (mamestra brassicae l.) with a mixture of nuclear polyhedrosis virus (npv) and cytoplasmic polyhedrosis virus (cpv), resulting in the impairment of virus assembly, and formation of abnormal nucleocapsids. at the same time protein supercapsids were produced normally, but contained no infectious virions. when insects were infected with related viruses, the virions developed as usual, but the protein supercapsids revealed abnorma ... | 1983 | 6139945 |
| molecular cloning of dna complementary to mrna of the baculovirus autographa californica nuclear polyhedrosis virus: location and gene products of rna transcripts found late in infection. | dnas complementary to late autographa californica nuclear polyhedrosis virus (acnpv) mrna were synthesized by reverse transcription and cloned in escherichia coli by using pbr322 as a vector. eleven different cdnas were distinguished in our screening of 45 acnpv-homologous clones. location of the regions of cdna homology with respect to the acnpv physical map showed that the 11 cdnas were dispersed throughout the genome. the most abundant cdna insertion, representing approximately one-third of t ... | 1982 | 6184487 |
| biological and biochemical investigations on five european isolates of mamestra brassica nuclear polyhedrosis virus. | five multiply enveloped european isolates of mamestra brassicae nuclear polyhedrosis virus (oxford, german, french, dutch and danish) were found to be very closely related serologically using the enzyme linked immunosorbent assay (elisa) double antibody sandwich method and immunodiffusion. by sds-polyacrylamide gel electrophoresis of viral proteins and restriction endonuclease analysis of dna using seven enzymes there appeared to be two variants as the oxford and german isolates were distinct fr ... | 1981 | 6271101 |
| replication of heliothis zea baculovirus in an insect cell line. | the baculovirus, heliothis zea nuclear polyhedrosis virus (hzsnpv), was grown in the h. zea cell line, iplb-1075. light microscopy observations showed that 95% of inoculated cells had cpe by 6 days postinoculation (p.i.). growth curves of intracellular and extracellular virus showed that maximum titers (tcid50 units) were reached 3-4 days p.i. studies on hzsnpv morphogenesis revealed that ultrastructural events were typical of baculovirus replication in vitro. ecori restriction enzyme analyses o ... | 1981 | 6276328 |
| the cloned ecori fragments of autographa californica nuclear polyhedrosis virus dna. | 1981 | 6282701 | |
| rifampin inhibition of the occluded virus form of a nuclear polyhedrosis virus. | rifampin at concentrations toxic to noninfected cells but not to infected cells is a selective inhibitor of occluded virus of the group a baculoviridae (nuclear polyhedrosis virus). however, the titer of nonoccluded virus is not affected. rifampin blocks occlusion until late in the replication cycle (14 to 16 h), and its effects are reversible. modes of action of polyhedral inclusion body production are unknown. | 1982 | 6291455 |
| analysis of a mutant of autographa california nuclear polyhedrosis virus with a defect in the morphogenesis of the occlusion body macromolecular lattice. | a mutant (m-29) of autographa californica nuclear polyhedrosis virus (acmnpv) grew in spodoptera frugiperda and trichoplusia ni cells but did not form typical intranuclear occlusion bodies (ob); instead, small particles (95 to 180 nm diam.) were produced in copious amounts within nuclei. ultrastructural studies showed that the particles did not occlude enveloped nucleocapsids and that they lacked a macromolecular paracrystalline lattice and a structure equivalent to the occlusion body envelope. ... | 1983 | 6306151 |
| production of human beta interferon in insect cells infected with a baculovirus expression vector. | autographa californica nuclear polyhedrosis virus (acnpv) was used as an expression vector for human beta interferon. by using specially constructed plasmids, the protein-coding sequences for interferon were linked to the acnpv promoter for the gene encoding for polyhedrin, the major occlusion protein. the interferon gene was inserted at various locations relative to the acnpv polyhedrin transcriptional and translational signals, and the interferon-polyhedrin hybrid genes were transferred to inf ... | 1983 | 6318086 |
| strong and regulated expression of escherichia coli beta-galactosidase in insect cells with a baculovirus vector. | the n-terminal region of the gene encoding polyhedrin, the major occlusion protein of the insect baculovirus autographa californica nuclear polyhedrosis virus (acnpv), has been fused to dna encoding escherichia coli beta-galactosidase. the fused gene was inserted into the acnpv dna genome by cotransfection of insect cells with recombinant plasmid dna and wild-type acnpv genomic dna. recombinant viruses were selected as blue plaques in the presence of a beta-galactosidase indicator, 5-bromo-4-chl ... | 1984 | 6325875 |
| the translational map of the autographa californica nuclear polyhedrosis virus (acnpv) genome. | we have mapped early and late viral gene products expressed in autographa californica nuclear polyhedrosis virus ( acnpv )-infected spodoptera frugiperda cells by cell-free translation of virus-specific rna which was selected by hybridization to cloned restriction endonuclease fragments of acnpv dna. proteins synthesized in vitro were labeled with [35s]methionine and analyzed by sds-polyacrylamide gel electrophoresis followed by fluorography. at least four early acnpv -specific polypeptides were ... | 1982 | 6327284 |
| midgut and viral associated proteases of heliothis armigera. | the role played by the gut juice of insects in the infective process of insect viruses was examined. analysis of larval gut extract of heliothis armigera by sds-page revealed protease activity associated with components of molecular weights 48,000 and 94,000. proteases were found to be associated with occlusion bodies and virions of both nuclear polyhedrosis virus (npv) and cytoplasmic polyhedrosis virus (cpv) infecting h. armigera. cpv occlusion bodies were dissolved by gut juice extract at ph ... | 1983 | 6337972 |
| neutralization studies on the autographa californica nuclear polyhedrosis virus. | 1983 | 6338869 | |
| morphogenesis of nuclear polyhedrosis virus from autographa californica in a cell line from mamestra brassicae (cabbage moth). further aspects on baculovirus assembly. | cultures of cell line izd-mb 0503 from mamestra brassicae were inoculated with nonoccluded nuclear polyhedrosis virus of autographa californica (acnov). at 1 h postinoculation (p.i.) nucleocapsids were found in the cytoplasm near nuclear pores and within the nucleoplasm. formation of virogenic stroma was observed at 7 h p.i. the first short empty capsids were seen at 10 h postinoculation (p.i.), followed by partially and completely filled nucleocapsids (11-12 h p.i.) with most capsids filled at ... | 1983 | 6339063 |
| monoclonal antibodies to baculovirus structural proteins: determination of specificities by western blot analysis. | conventional mouse hybridoma technology was utilized to produce a panel of monoclonal antibodies which reacted with baculovirus proteins. using an enzyme-linked immunosorbent assay (elisa), the hybridomas which were raised against polyhedrin from autographa californica nuclear polyhedrosis virus (acnpv) and choristoeura fumiferana nuclear polyhedrosis virus (cfnpv) were found to cross-react differentially with polyhedrins and granulins from several species of baculoviruses. hybridoma antibodies ... | 1983 | 6340331 |
| the influence of larval maturation on responses of mamestra brassicae l. (lepidoptera: noctuidae) to nuclear polyhedrosis virus infection. | responses of mature mamestrae brassica larvae, in the fifth and sixth instars, to nuclear polyhedrosis virus (npv) have been quantified. there was an 86 fold decrease in susceptibility to virus, related linearly to increasing body weight, for the larval weight range tested. although the rate of decreasing response to weight was constant, three phases of susceptibility were identified by shifts in the positions of the regression line of log ld50 on log body weight. larvae above 700 mg were propor ... | 1983 | 6340641 |
| baculovirus replication: inhibition of trichoplusia ni multiple nuclear polyhedrosis virus by [e]-5-(2-bromovinyl)-2'-deoxyuridine. | [e]-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) inhibits the replication of the baculovirus trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus in spodoptera frugiperda cells. virus-specific dna synthesis and late protein synthesis are suppressed by the drug. bvdu is phosphorylated by deoxythymidine (deoxycytidine) kinase present in both uninfected and virus-infected cells, and in its 5'-triphosphate form it inhibits dna polymerase activity in virus-infected cells. the effect of the bvd ... | 1983 | 6343553 |
| safety evaluation of nuclear polyhedrosis virus replication in pigs. | to evaluate the hygienic risk involved in using baculoviruses for insect pest control, safety studies are required. pigs were chosen as representative test animals of commercial and agricultural importance. the tests were aimed at detecting virus propagation, immune reactions, and signs of acute infection (changes in body temperature and hematology profile, swelling of lymph nodes). four of five animals inoculated with nuclear polyhedrosis virus showed a slight temperature rise at day 2 postinfe ... | 1983 | 6344789 |
| persistent baculovirus infections: spodoptera frugiperda npv and autographa californica npv in spodoptera frugiperda cells. | establishment of a persistent infection of spodoptera frugiperda nuclear polyhedrosis virus (npv) in spodoptera frugiperda (s.f.) cells occurred in three phases: the first phase was characterised by high levels of cell infection and death, the second phase by decreasing cell infection levels leading to the final phase where less than one per cent of the cells were infected during any subculture. the virus persisted at this level of infection provided the cells were maintained by regular subcultu ... | 1983 | 6360080 |
| photoreactivation and ultraviolet-enhanced reactivation of ultraviolet-irradiated nuclear polyhedrosis virus by insect cells. | the nuclear polyhedrosis virus (baculovirus) of galleria mellonella (pryalidae: lepidoptera) was used to investigate the capability of cultured insect cells to repair ultraviolet (uv) induced damage in the viral genome. when assayed by the formation of plaques in the cell line tn-368, the survival of the virus was found to decrease linearly with increased ultraviolet exposure. the infectious capacity of uv-irradiated virions was significantly restored after exposing the tn-368 monolayers to eith ... | 1984 | 6365037 |
| nucleotide sequence of the galleria mellonella nuclear polyhedrosis virus origin of dna replication. | the initiation sites of the galleria mellonella l. nuclear polyhedrosis virus (g.m. npv) dna replication were revealed. for this purpose scld 135 cells permitting the g.m. npv productive reproduction were transformed by the recombinant plasmids containing the viral genome individual fragments in prsf 2124 and pbr 322 vectors. it was revealed that 2 of the 32 recombinant plasmids can autonomously replicate in the eucaryotic cells. according to the maxam-gilbert method the dna g.m. npv fragment (1 ... | 1984 | 6365591 |
| replication and serial passage of a singly enveloped baculovirus of orgyia leucostigma in homologous cell lines. | a singly enveloped nuclear polyhedrosis virus (snpv) of the white-marked tussock moth, orgyia leucostigma, was successfully grown in four continuous cell lines developed from minced neonate larvae of this insect. level of infection in two cell lines, ipri-ol-12 and ipri-ol-13, was 65-90%, but in the other two, ipri-ol-4 and ipri-ol-9, it was about 3%. polyhedral inclusion bodies (pibs) appeared in the nuclei of cells within 24 h postinoculation. cytopathological changes and morphogenesis of the ... | 1984 | 6365830 |
| identification and localization of reiterated sequences in the choristoneura fumiferana mnpv genome. | the genome of choristoneura fumiferana nuclear polyhedrosis virus (cfmnpv) contained reiterated sequences interdispersed in four locations. these regions, termed rs, were found in ecori fragments a, f, e and b. the sequences were identified by hybridization of the fragment ecori-a to a southern blot of ecori-digested viral dna. further confirmation and more precise localization of the rs sequences was obtained by hybridization of nick-translated 32p-labeled ecori-e fragment to southern blots of ... | 1984 | 6370684 |
| interaction of autographa californica nuclear polyhedrosis virus with two nonpermissive cell lines. | the interaction of autographa californica nuclear polyhedrosis virus with two nonpermissive cell lines was investigated. the insect cell line, cp 169, and the chinese hamster cell line, cho-k1, were able to adsorb and engulf virus particles, but there was no evidence for viral replication in these cells based on virus growth titrations, electron microscopy, dot hybridization, and synthesis of viral induced proteins. | 1984 | 6376420 |
| baculovirus replication: phosphorylation of polypeptides synthesized in trichoplusia ni nuclear polyhedrosis virus-infected cells. | a number of polypeptides synthesized specifically in trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus (t. ni mnpv)-infected spodoptera frugiperda cells are phosphorylated both early and late in infection. certain non-structural proteins and the major basic internal protein are the main phosphoproteins detected in infected cells. the polyhedron protein was not phosphorylated. many cell proteins continue to be phosphorylated throughout infection. pulse-chase experiments have shown ... | 1984 | 6379101 |
| production of polyhedrin monoclonal antibodies for distinguishing two orgyia pseudotsugata baculoviruses. | monoclonal antibodies were produced to polyhedrins from orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (opmnpv) and single-capsid nuclear polyhedrosis virus (opsnpv). although the polyhedrins are closely related, antibodies were selected which allowed differentiation between the two viruses. in an indirect enzyme-linked immunosorbent assay, purified opmnpv and opsnpv polyhedrins could be detected by specific monoclonal antibodies at concentrations as low as 2 and 5 ng/ml, respective ... | 1984 | 6391377 |
| [physico-chemical characteristics of the dna of the nuclear polyhedrosis virus of the moth porthetria dispar l]. | dna preparations were obtained after dissolving the inclusion bodies, polyhedra virus particles, from the purified bundle virus of porthetria dispar l. nuclear polyhedrosis. the dna molecules in the preparations obtained are of different conformation and separate within the cscl density gradient in the presence of ethidium bromide into supercoiled catenated and relaxed circular molecules (with the admixture of linear molecules). the circular dna was studied by electron microscopy. the size of vi ... | 1984 | 6393473 |
| autographa californica nuclear polyhedrosis virus (acnpv) dna does not persist in mass cultures of mammalian cells. | autographa californica nuclear polyhedrosis virus (acnpv) is one of the most extensively studied baculoviruses. we have investigated whether acnpv or its dna can replicate and/or persist in cultures of mammalian cells. human hela cells or primary human embryonic kidney cells, simian cv1 cells, hamster bhk21 (b3) cells or muntiacus muntjak cells growing in monolayer cultures were used in these studies. cells were inoculated with acnpv at multiplicities ranging from 0.1 to 100 pfu/cell. subsequent ... | 1983 | 6402854 |
| bromodeoxyuridine-induced mutants of autographa californica nuclear polyhedrosis virus defective in occlusion body formation. | twelve temperature-sensitive (ts) and two morphology mutants of autographa californica nuclear polyhedrosis virus were generated using the mutagen 5-bromo-2'-deoxyuridine (brdurd). the ts mutants grew normally at 25 degrees c but exhibited abnormal occlusion body formation when grown at 33 degrees c whereas the morphology mutants produced an abnormal cytopathic effect at 25 degrees c and 33 degrees c. none of the mutants was severely restricted for production of non-occluded virus; thus, with th ... | 1982 | 6754865 |
| the use of a protein a conjugate in an indirect enzyme-linked immunosorbent assay (elisa) of four closely related baculoviruses from spodoptera species. | the virus particles from four closely related insect nuclear polyhedrosis virus (npvs) isolated from their homologous spodoptera spp. hosts were examined using an indirect enzyme-linked immunosorbent assay (elisa), employing both swine anti-rabbit and protein a conjugate. results from both systems indicated that s. littoralis npv is serologically distinct from the other three viruses, s. frugiperda npv, s. exempta npv and s. exigua npv, which are closely related. the relatedness of these viruses ... | 1982 | 6754866 |
| transcription of the autographa californica nuclear polyhedrosis virus genome: location of late cytoplasmic mrna. | 1982 | 6755883 | |
| effect of aluminum chloride and zinc sulfate on autographa california nuclear polyhedrosis virus (acnpv) replication in cell culture. | when ipl-sf-21ae iii continuous insect cell line was grown and maintained in ipl-41 insect cell culture medium supplemented with 16 microm of alcl3 or 0.24 microm of znso4 . 7h2o, or both metallic salts, and then infected with autographa california nuclear polyhedrosis virus, virus replication was increased significantly. the yield of polyhedral inclusion bodies (pib) was enhanced up to 121%. synthesis of cell-free nonoccluded virus was increased to 365% when infectivity was assayed by the plaqu ... | 1982 | 6759370 |
| baculovirus induction of a dna polymerase. | the baculovirus, autographa california nuclear polyhedrosis virus, induced a new aphidicolin-sensitive, alpha-like, dna polymerase upon infection of the lepidopteran noctuid, trichoplusia ni. the new virus-induced dna polymerase could be separated from the host alpha-like polymerase by phosphocellulose chromatography. the two polymerases differed in their sensitivities to heat inactivation, high salt concentrations, and 0.1 m phosphate buffer. | 1981 | 6793735 |
| virulence of cloned variants of autographa californica nuclear polyhedrosis virus. | the relative virulence of five different genotypic variants of autographa californica nuclear polyhedrosis virus was tested by determining the 50% lethal dose of occluded virus for larvae of trichoplusia ni. the 50% lethal dose values of uncloned virus and the five cloned genotypic variants ranged between 10 and 21 polyhedra per larva, and no statistically significant differences were observed. cloning has therefore neither enhanced nor decreased the virulence of this potential microbial pestici ... | 1980 | 6990868 |
| the infectivity of polyhedra of nuclear polyhedrosis virus (n.p.v.) after passage through gut of an insectpredator. | polyhedral inclusion bodies (p.i.b) of a nuclear polyhedrosis virus of heliothis punctigera (lep.) were found in excreta of nabis tasmanicus (het.), a predator of h. punctigera. an immunofluorescent counting method was used to estimate numbers of p.i.b. bioassays demonstrated that no loss of infectivity occurred during passage through the gut. | 1980 | 6995145 |
| [degradation of silkworm nuclear polyhedrosis virus]. | 1980 | 6995813 | |
| biological studies of a baculovirus in a mammalian cell line. | in two of five experiments, replication (tcid50) was observed following inoculation of chinese hamster cells with autographa californica nuclear polyhedrosis virus (npv). radiolabeling experiments employing tritiated thymidine consistently resulted in a labeled entity which banded in sucrose gradients at a density of 1.24-1.25 g/ml, similar to that observed for a. californica npv in the permissive trichoplusia ni cell line. the labeled entity is believed to be a. californica npv, based on infect ... | 1980 | 6998919 |
| [quantitative determination of the infectivity of nuclear polyhedrosis virus dna on honeycomb moth (galleria mellonella) larvae]. | the possibility of using honeycomb moth larvae for titration of nuclear polyhedrosis virus (npv) infectious dna and determinations of transfection effectiveness was studied. honeycomb moth larvae were shown to be a sensitive system for npv dna titration. deae-dextran used as a protector increased npv dna infectivity 1000-fold, ld50 in this instance being 2 x 10(8) molecules per larva. the method of npv dna infectivity determinations by the number of larvae with polyhedreae in the fatty tissue is ... | 1980 | 7001752 |
| biochemical and biophysical properties of a mamestra brassicae multiple enveloped nuclear polyhedrosis virus. | a multiply enveloped nuclear polyhedrosis virus from mamestra brassicae has been shown to be morphologically similar to other baculoviruses. the virus particles contain 13 polypeptides of which 4 are associated with the nucleocapsid. the polyhedron comprises of one major polypeptide of molecular weight 28,000. the dna has a mol. wt. of about 1.15 x 10(8), which is larger than that reported for other baculoviruses. the dna is a circular, supercoiled molecule of cg mol. fraction 0.448. dna fragmen ... | 1980 | 7002114 |
| [virion polypeptides of the nuclear polyhedrosis virus of the silkworm]. | the nuclear polyhedrosis virus of the silkworm b. mori was obtained by dissolution of polyhedra at ph 10,5. it was shown that the virus particles contain a protease, which cleaves a few virion polypeptides during the dissolution of polyhedra under alkaline conditions. the proteolytic activity is inactivated by polyhedra treatment with phenylmethylsulfonylfluoride, diisopropylfluorophosphate, hgcl2 and at 80 degrees. sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed 18 virion pol ... | 1980 | 7011425 |
| field-flow fractionation of alkali-liberated nuclear polyhedrosis virus from gypsy moth lymantria dispar linnaeus. | rod-shaped viral particles of the gypsy moth nuclear polyhedrosis virus (npv) were obtained through alkaline dissolution of inclusion bodies isolated and purified from infected hosts. the liberated viral fraction contains a wide assortment of enveloped aggregated forms as well as enveloped monomers. this complex mixture was separated by means of sedimentation field-flow fractionation (sedimentation fff) to give a mass spectrum of the separated particles. from the elution pattern effective molecu ... | 1980 | 7014576 |
| virus envelope acquisition of nuclear polyhedrosis virus in vitro. | there have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. while there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. three possible ways have been proposed to describe envelope formation of nuclear polyhedrosis virus: (i) acquisition of budding ... | 1981 | 7018096 |
| [pathohistological and ultrastructural studies of the nuclear polyhedrosis virus in the silkworm (bombyx mori l.)]. | histopathologic studies were carried out on fifth instar silkworm moths of the super 1 x super 2 hybrid infected orally with baculovirus bombycis along with ultrastructural investigations of the pathogen itself. it was found that the earliest changes in the moths set in at the 60th hour post inoculation. they consisted in the aggregation of chromatin, and the formation of polyhedral bodies in the fatty body, hypodermis, and peritracheal matrix; these appeared in the glandular tissue too, but not ... | 1980 | 7020236 |
| effects of red-headed in sawfly, neodiprion lecontei, nuclear polyhedrosis virus on rainbow trout, salmo gairdneri, and daphnia pulex. | the effects of a nuclear polyhedrosis virus (npv) of the red-headed pine sawfly, neodiprion lecontei, on rainbow trout, salmo gairdneri, were investigated. the fish were exposed to this virus by intubation and topical application and no ill-effects were observed. similarly, no ill-effects were detected in daphnia pulex when the same npv was added to their culture medium. the materials were lyophilized, npv-infected sawfly larvae (normally used for insect control), lyophilized, uninfected larvae ... | 1981 | 7026655 |
| improved replication of autographa californica nuclear polyhedrosis virus in roller bottles: characterization of the progeny virus. | a reproducible growth curve was established for the propagation of autographa californica (speyer) nuclear polyhedrosis virus (acmnpv) in a continuous insect line from spodoptera frugiperda (j. e. smith) during large-volume production. a newly developed method for quantitation of polyhedra inclusion bodies (pib) by electron microscopy (em) during the growth cycle was compared to hemocytometer counts. the virus particles (vp) ratio to pfu and vp per pib were established by em methods. optimal yie ... | 1981 | 7028679 |
| baculovirus bioassay not dependent upon polyhedra production. | an immunoperoxidase-based quantitative assay has been developed for infectious units of autographa californica nuclear polyhedrosis virus infecting tn-368 cells. major benefits of this assay over existing quantitative assays for baculoviruses are that it is not dependent upon polyhedra production, it is clear-cut and easy to score, and results are obtained within 24 h. | 1981 | 7028916 |
| growth of nuclear polyhedrosis virus in larvae of the cabbage moth, mamestra brassicae l. | growth of nuclear polyhedrosis virus (npv) in 5 larval instars of cabbage moth, mamestra brassicae, has been quantified using 2 methods. numbers of polyhedra were estimated by light microscope counts while concentrations of virus protein antigen were estimated using elisa. virus growth was rapid initially but slowed during its later stages, although elisa protein concentrations decreased once a peak had been reached. there was a linear correlation between polyhedral counts and virus protein duri ... | 1981 | 7034687 |
| [transfection conditions for baculovirus dna in an insect cell line]. | the following conditions were found to be necessary to achieve infection of a spodoptera frugiperda cell line with purified dna of nuclear polyhedrosis virus of spodoptera exempta (npv): (1) cell monolayers shall be prepared one day before the experiment; the number of cells added to 35-mm wells of plastic clusters (falcon) shall be 1.0 x10(6)-1.1x10(6), and the cells shall be in the stage of logarythmic growth; dna shall be diluted in hebs-buffer (ph 6.95-7.05); the volume of the mixtures added ... | 1982 | 7090350 |