Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| nystagmus induced by electrical stimulation of the vestibular and prepositus hypoglossi nuclei in the monkey: evidence for site of induction of velocity storage. | electrical stimulation of the vestibular nuclei (vn) and prepositus hypoglossi nuclei (pph) of alert cynomolgus monkeys evoked nystagmus and eye deviation while they were in darkness. at some sites in vn, nystagmus and after-nystagmus were induced with characteristics suggesting that velocity storage had been excited. we analyzed these responses and compared them to the slow component of optokinetic nystagmus (okn) and to optokinetic after-nystagmus (okan). we then recorded unit activity in vn a ... | 1992 | 1486947 |
| penetration of zidovudine and 3'-fluoro-3'-deoxythymidine into the brain, muscle tissue, and veins in cynomolgus monkeys: relation to antiviral action. | cynomolgus monkeys had microdialysis probes implanted under ketamine anesthesia into peripheral veins, thigh muscles, and the brain in order to sample the extracellular fluid for the concentrations of unbound nucleoside analogs. a dose of 25 mg of zidovudine or 3'-fluoro-3'-deoxythymidine (flt) per kg was administered subcutaneously to each of three animals. relatively high antiviral concentrations of flt and zidovudine were present in peripheral tissues and in the brain. it was found that the c ... | 1992 | 1489185 |
| simian immunodeficiency virus (sivsm) infection of cynomolgus monkeys: effects on follicular dendritic cells in lymphoid tissue. | we studied follicles in sections of lymph nodes and spleen from cynomolgus monkeys (macaca fascicularis) after infection with simian immunodeficiency virus (sivsm), by (immuno)histology and (immunogold) electron microscopy. also isolated follicular dendritic cells (fdc) were investigated. histology showed ranged from follicular hyperplasia to follicle fragmentation. fdc showed desmin and vimentin, characteristic of mesenchymal cells. except for two animals who got experimental chemotherapy in th ... | 1992 | 1493052 |
| [comparative radiation sensitivity of monkeys, macaca rhesus and macaca fascicularis]. | 1992 | 1496108 | |
| cytokine gene expression in rejecting cardiac allografts. | heart transplantation is now a viable therapeutic option for patients with certain end-stage cardiac diseases. however, episodes of rejection, opportunistic infection, and life-threatening side effects of generalized immunosuppression remain very real problems for these patients. a better understanding of the molecular mechanisms underlying rejection may provide the basis for the development of more specific, less toxic immunosuppressive therapies. while cytokines have long been implicated in th ... | 1992 | 1496544 |
| acute morphine induces oscillatory discharge of noradrenergic locus coeruleus neurons in the waking monkey. | neurons were recorded extracellularly from the locus coeruleus (lc) of a waking, chair-restrained cynomolgus monkey before and for 0.5-4 h after intramuscular injections of morphine sulfate (0.3-10 mg/kg). tonic discharge of each lc neuron tested (n = 11) decreased after morphine injection; this effect appeared to be dose-dependent for the range of 0.3-3.0 mg/kg. unexpectedly, these same doses of morphine also induced a pronounced burst-pause discharge pattern in all lc neurons recorded. thus, w ... | 1992 | 1501782 |
| [evaluation of 5 years of experimental in utero surgery for the repair of diaphragmatic hernia]. | the incidence of diaphragmatic hernia in he newborn is 1:2,500 to 5,000 births. an extensive american programme of foetal surgery for in utero repair of this defect has shown that the pulmonary hypoplasia was due to compression of lung tissue by the herniated organs. this process could be interrupted be repairing the diaphragmatic defect early enough in utero (before the 28th week). the results of five years of experimental surgery for in utero correction of diaphragmatic hernia are presented he ... | 1992 | 1503293 |
| the immunohistochemical localization of new membrane-associated placental tissue proteins (mp2 a, b, c, d, and e) in human and cynomolgus monkey placentae. | new membrane-associated placental tissue proteins (mp2 a, b, c, d, and e) were investigated by avidin-biotin immunoperoxidase technique in the human and cynomolgus monkey placentae, decidua and umbilical cords. in human early placentae, mp2 a, b, c, and e were localized mainly in the membrane of villous syncytiotrophoblasts and cytotrophoblasts. histiocytes in the villous stroma were positive for mp2 a, b, d, and e. in human term placentae, obvious positive staining for mp2 a, b, c, and e was ob ... | 1992 | 1503540 |
| dna sequence analysis of spontaneous and n-ethyl-n-nitrosourea-induced hprt mutations arising in vivo in cynomolgus monkey t-lymphocytes. | the study of hprt mutations in cynomolgus monkey t-lymphocytes is part of our effort to understand the mechanisms of mutagenesis in vivo. this primate model allows us to study mutations and their kinetics at the molecular level under well-controlled conditions using recently developed techniques for selection of mutant t-cells and polymerase chain reaction (pcr) amplification of hprt cdna, which is directly sequenced. this is the first report of the sequence of the coding region of the cynomolgu ... | 1992 | 1505533 |
| neuropeptide y in the primate model of subarachnoid hemorrhage. | the cause of cerebral vasospasm after subarachnoid hemorrhage (sah) remains unknown. recently, an association between the potent vasoconstricting peptide, neuropeptide y, and delayed cerebral vasospasm after sah has been postulated. this was based on the findings of increased neuropeptide y levels in the cerebrospinal fluid (csf) and plasma after sah in animals and humans. for this study, the primate model of sah was used to assess the possible role of neuropeptide y in delayed vasospasm after s ... | 1992 | 1506889 |
| projections from cortical visual areas of the superior temporal sulcus to the superior colliculus, in macaque monkeys. | 1. the distribution of tectal projections of two visual areas of the superior temporal sulcus (mt and mst areas) has been studied, in five macaca fascicularis, by means of the autoradiographic method tracing the anterograde transport of tritiated aminoacids intracortically injected. 2. in all cases the ipsilateral superior colliculi (sc) were found labelled, whereas the contralateral ones were devoid of label. 3. the three brains injected in the mt area resulted in sc labels that involved the su ... | 1992 | 1510547 |
| effects of central administration of opioids on facial scratching in monkeys. | epidural and intrathecal administration of opioids to humans can produce facial pruritus and scratching that is naloxone reversible. it has been proposed that opioids may act at the level of the medulla to produce facial pruritus and associated scratching behavior. we investigated the effects of mu, delta and kappa opioid-receptor agonists microinjected unilaterally into the medullary dorsal horn (mdh) on facial scratching in cynomolgus monkeys. the selective mu opioid-receptor agonist, damgo (3 ... | 1992 | 1511314 |
| a-71623, a selective cck-a receptor agonist, suppresses food intake in the mouse, dog, and monkey. | the anorectic actions of cholecystokinin (cck)-8 and of a selective cck-a agonist, a-71623, were examined in cd1 mice, beagle dogs, and cynomolgus monkeys. a-71623 suppressed intakes in all species tested, and the effects were blocked by a selective cck-a antagonist, a-70104. in the dog only, cck-8 was more potent on a molar basis compared to a-71623, although the effects of both cck agonists were more short-lived in the dog compared to the other species tested. our results support other evidenc ... | 1992 | 1513850 |
| 3-o-methyldopa administration does not alter fluorodopa transport into the brain. | to determine if 3-o-methyldopa (3omd) significantly inhibits the transport of 6-[18f]fluorodopa (6-fd) into the brain at the concentration normally encountered during l-dopa administration, we performed 6-fd studies with positron emission tomography in cynomolgus monkeys in the presence and absence of 3omd. infusion of 3omd was designed to produce plasma concentrations equivalent to those seen in patients on chronic l-dopa therapy. plasma 3omd levels of 39 +/- 4 microm did not alter the blood-br ... | 1992 | 1514775 |
| viable adrenal medullary transplants in non-human primates: increasing the number of grafts. | the robust survival of stereotaxic adrenal medullary autografts in monkey brain parenchyma depends heavily on technique. one aspect of technique critical for clinical applications of cns grafting is the problem of spreading treatment effects throughout large regions in a primate brain. stereotaxic placement of a large number of grafts, which would address this problem, would require that later grafts retain the capacity for viability over a long period of time during the surgery, while earlier g ... | 1992 | 1515484 |
| behavioral effects of adrenal medullary transplants in non-human primates. | small multiple "ribbon" autografts of intact adrenal medulla stereotaxically implanted at several sites throughout the striatum in longtailed macaques (macaca fascicularis) have been shown to contain large amounts of viable glandular tissue as long as eight weeks after transplantation. variations of technique clearly influence viability. all monkeys were maintained in specially adapted rotometer cages so that 24-hour measurements of activity and directional bias could be gathered. lesions induce ... | 1992 | 1515485 |
| spinal and trigeminal lamina i input to the locus coeruleus anterogradely labeled with phaseolus vulgaris leucoagglutinin (pha-l) in the cat and the monkey. | terminal fibers anterogradely labeled with phaseolus vulgaris leucoagglutinin (pha-l) were observed in the locus coeruleus in the brainstem of the cat and the cynomolgus monkey following injections in lamina i of the spinal or medullary dorsal horn. thus, thermoreceptive- or nociceptive-specific lamina i cells that project to the locus coeruleus could directly influence arousal, vigilance, and the descending control of spinal integration. | 1992 | 1515950 |
| thyrotropin-releasing hormone (trh)-like immunoreactivity in the grey monkey (macaca fascicularis) spinal cord and medulla oblongata with special emphasis on the bulbospinal tract. | the distribution of thyrotropin-releasing hormone (trh)-like immunoreactivity (li) has been studied in the grey monkey (macaca fascicularis) spinal cord and medulla oblongata by the use of indirect immunofluorescence and the peroxidase-antiperoxidase (pap) technique. furthermore, double-labeling experiments were performed in order to study colocalization of 5-hydroxytryptamine (5-ht)- and substance p-li. a dense innervation of trh-immunoreactive (ir) varicose fibers was found in the ventral horn ... | 1992 | 1517482 |
| influence of a gonadotrophin-releasing hormone agonist and gonadotrophins on morphometric characteristics of the population of small ovarian follicles in cynomolgus monkeys (macaca fascicularis). | small follicles, less than or equal to 100 microns, in monkey ovaries were divided into four types based on the morphological characteristics of the granulosa cells that surrounded the oocyte: primordial, intermediary, primary and secondary follicles. the proportion of primordial follicles positively correlated, whereas those of intermediary, primary and secondary follicles negatively correlated, with the total number of follicles less than or equal to 100 microns. there was no relationship betw ... | 1992 | 1518011 |
| immunohistochemical localization of hyaluronan synthase in cornea and conjunctive of cynomolgus monkey. | distribution of hyaluronan synthase was investigated in cornea and conjunctiva of cynomolgus monkeys (macaca fascicularis) using polyclonal antibodies against the streptococcal enzyme. strong immunoreaction was found in the cell membranes of the corneal endothelium, corneal epithelium, and most of the conjunctival epithelium. in the corneal epithelium all cells except the basal ones stained. in the conjunctiva all cylindrical cells stained, whereas among the goblet cells one type showed intense ... | 1992 | 1521573 |
| behavioral effects of lead in monkeys tested during infancy and adulthood. | a total of 12 monkeys (macaca fascicularis) were dosed orally from birth with 0 or 2000 micrograms/kg/day of lead as lead acetate. blood lead concentrations of treated monkeys peaked at an average of 115 micrograms/dl by 100 days of age and decreased to a steady state level of 33 micrograms/dl after withdrawal of infant formula at 270 days of age. at 5-6 months of age, they were tested on a nonspatial discrimination reversal paradigm. at 2.5-3.0 years of age, they were tested on a series of nons ... | 1992 | 1522828 |
| drug-metabolizing enzymes in respiratory nasal mucosa and liver of cynomolgus monkey. | 1. microsomal and cytosolic drug-metabolizing enzyme activities of respiratory mucosa of male and female monkeys have been determined and compared to those of monkey liver. the results demonstrated that cytochrome p-450, nadph-cytochrome p-450 reductase and some monooxygenase activities, especially ethoxycoumarin o-deethylase activity, were present in respiratory epithelium, although at lower levels than in liver. 2. activities of non-oxidative enzymes--namely, epoxide hydrolase, udp-glucuronylt ... | 1992 | 1523863 |
| 13-week inhalation toxicity study of dimethylformamide (dmf) in cynomolgus monkeys. | this study was conducted to assess the subchronic inhalation toxicity of dimethylformamide (dmf) in the cynomolgus monkey. particular attention was paid to the liver since dmf has been shown to produce liver damage in rodents, dogs, and humans. groups of three cynomolgus monkeys/sex/group received whole-body exposures for 6 hr/day, 5 days/week for 13 weeks to 0, 30, 100, or 500 ppm dmf. evaluations for toxicity included body and organ weights, clinical observations, hematology, serum chemistry, ... | 1992 | 1526372 |
| pathogenic potential of filoviruses: role of geographic origin of primate host and virus strain. | african filoviruses have caused outbreaks of fulminating hemorrhagic fever among humans. in 1989, related filoviruses were isolated from cynomolgus monkeys imported into the united states from the philippines. the pathogenic potential of these new filoviruses was compared in 16 asian monkeys (macaca fascicularis-cynomolgus) and 16 african monkeys (cercopithecus aethiops-african green) using african filoviruses from zaire (ebola virus) and sudan or asian filoviruses (reston and pennsylvania). afr ... | 1992 | 1527410 |
| dietary polyunsaturated to saturated fatty acid ratio alters hepatic ldl transport in cynomolgus macaques fed low cholesterol diets. | to determine effect of interaction between dietary cholesterol and triglyceride, i.e., polyunsaturated to saturated (p:s) fatty acid ratio, on ldl metabolism, male cynomolgus macaques were fed purified diets for 83 wk with cholesterol levels of 0.01, 0.06 and 0.50 mg/kj and p:s ratios of 0.5 and 0.9, oleic acid constant. there were six groups of five animals each (cholesterol, mg/kj--p:s ratio): group 1, 0.01--0.5; group 2, 0.01--0.9; group 3, 0.06--0.5; group 4, 0.06--0.9; group 5, 0.50-0.5; gr ... | 1992 | 1527637 |
| protection of macaques against siv infection by subunit vaccines of siv envelope glycoprotein gp160. | simian immunodeficiency virus (siv) is a primate lentivirus related to human immunodeficiency viruses and is an etiologic agent for acquired immunodeficiency syndrome (aids)-like diseases in macaques. to date, only inactivated whole virus vaccines have been shown to protect macaques against siv infection. protective immunity was elicited by recombinant subunit vaccines. four macaca fascicularis were immunized with recombinant vaccinia virus expressing sivmne gp160 and were boosted with gp160 pro ... | 1992 | 1531159 |
| effects of subchronic infusion of dehydroepiandrosterone sulfate on serum gonadotropin levels and ovarian function in the cynomolgus monkey. | to assess the impact of elevated adrenal androgen levels on ovarian function in a nonhuman primate using a repeated measures experimental design. | 1992 | 1532562 |
| early appearance of simian immunodeficiency virus (siv) antigen and antibodies as variables in evaluating antiviral drugs in macaques. | siv infection in macaques has become an important animal model for hiv-1 infection in humans. an antibody assay was therefore developed and compared to a commercially available antigen assay with respect to their usefulness to monitor the course of simian immunodeficiency virus (siv) infection in cynomolgus monkeys. a peptide, jb6t, consisting of 21 amino acids with the sequence nswgcafrqvchttvpwvnds corresponding to a segment in the env protein of human immunodeficiency virus (hiv) type 2 was u ... | 1992 | 1533400 |
| intravenous injection of albunex microspheres causes thromboxane mediated pulmonary hypertension in pigs, but not in monkeys or rabbits. | intravenous injection of the ultrasound contrast agent albunex (manufactured by nycomed as, oslo, norway; 400 million air-filled albumin microspheres per ml, mean diameter 4 +/- 1 microns) caused a dose-dependent increase of mean pulmonary arterial pressure in nine pigs. the highest dose (0.014 +/- 0.002 ml kg-1) increased mean pulmonary arterial pressure from 17 +/- 1 mmhg to 42 +/- 3 mmhg and decreased mean systemic arterial pressure from 111 +/- 9 to 93 +/- 12 mmhg. the pressure responses beg ... | 1992 | 1533987 |
| light and electron microscopic morphology of the temporomandibular joint in growing and mature crab-eating monkeys (macaca fascicularis): the condylar calcified cartilage. | in an attempt to show maturational alterations in the calcified cartilage, mandibular condyles of four growing and four adult male monkeys (macaca fascicularis) were studied using light microscopy as well as transmission and scanning electron microscopy. all specimens were initially fixed by perfusion in the presence of ruthenium red. for examination of the hard tissue surfaces in the scanning electron microscope, uncalcified tissues were removed with sodium hypochlorite. in growing animals, alm ... | 1992 | 1536452 |
| effects of orchidectomy on gonadotropin and inhibin subunit messenger ribonucleic acids in the pituitary of the rhesus monkey (macaca mulatta). | our research programs required the preparation of hypophysectomized and orchidectomized rhesus monkeys. this afforded us the possibility to characterize and compare levels of the gonadotropin and inhibin subunit mrnas in pituitaries from intact and castrate monkeys. eighteen adult male monkeys, four of which had been bilaterally orchidectomized 5-9 months previously, were used in this study. plasma concentrations of lh and fsh were, respectively, 188.5 +/- 5.3 and 246.8 +/- 25.2 ng/ml in the cas ... | 1992 | 1537290 |
| stimulating erythropoiesis increases complement receptor expression on primate erythrocytes. | erythrocyte complement receptors (cr) are unique to primates and play a role in the clearance of immune complexes from the circulation. immune complex-mediated diseases (e.g., systemic lupus erythematosus, sle) cause decreased erythrocyte cr levels, resulting in decreased capacity of the erythrocytes to bind immune complexes that form in the circulation. thus, raising erythrocyte cr levels might benefit patients with immune complex-mediated diseases. because young erythrocytes express more cr th ... | 1992 | 1541055 |
| purification of two cytochrome p450 isozymes related to cyp2a and cyp3a gene families from monkey (baboon, papio papio) liver microsomes. cross reactivity with human forms. | two cytochrome p450 isozymes, fa and fi, were isolated and characterized from liver microsomes of phenobarbital-induced baboons (papio papio). the cytochrome fa possesses the same n-terminal amino acid sequence as p450 mk2 from crab-eating monkeys (macaca irus) and closely resembles the human p450 3a isozymes. this cytochrome was able to oxidize nifedipine and hydroxylate testosterone at the 6 beta position. the second baboon cytochrome (fi) is closely related to the p450 2a subfamily and has th ... | 1992 | 1541278 |
| effect of estrogen and progesterone on l-dopa induced dyskinesia in mptp-treated monkeys. | a group of 4 cynomolgus monkeys was rendered parkinsonian by the toxin mptp and then treated daily with l-dopa until all animals developed dyskinesia. at this point, 17 beta-estradiol, 17 alpha-estradiol and progesterone were injected s.c. singly or in combination for 7 days and the treatment with l-dopa reinstituted. our results show that the pretreatment with 17 beta-estradiol reduced the dyskinetic effect without altering the therapeutic response to l-dopa, while 17 alpha-estradiol did not al ... | 1992 | 1542429 |
| metabolic activation and genotoxicity of heterocyclic arylamines. | because of the potential for human exposure to mutagenic and carcinogenic heterocyclic arylamines in the diet, the carcinogenicity of three haas, 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,8-dimethyl-imidazo[4,5-f]quinoxaline, and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, is being evaluated in nonhuman primates, especially cynomolgus monkeys. concomitant with the carcinogenicity studies, the metabolic processing, disposition, and dna-adduct formation of these compounds are being e ... | 1992 | 1544147 |
| distribution of calbindin d28k-like immunoreactivity (li) in the monkey ventral horn: do renshaw cells contain calbindin d28k-li? | by use of indirect immunofluorescence and peroxidase-antiperoxidase immunohistochemistry, we show that the monkey (macaca fascicularis) ventral horn harbors small- to medium-sized neurons in lamina vii as well as a dense network of fibers in laminae ix and vii that contain calbindin d28k (calbindin)-like immunoreactivity. the highest frequency of immunoreactive (ir) cell bodies was found at the levels of the cervical and lumbar intumescences. furthermore, rostrocaudally oriented calbindin-ir fib ... | 1992 | 1545236 |
| reinnervation of distal sensory nerve environments by regenerating sensory axons. | this study investigated the specificity of sensory nerve regeneration in a primate model. in adult cynomolgus monkeys, the femoral nerve was explored in the groin and two sensory branches identified. a sensory branch was sectioned and introduced into the proximal channel of a y-shaped silicone chamber. this proximal sensory nerve stump was given distal choices of distal sensory nerve graft or distal sensory nerve which was intact to the distal sensory cutaneous receptors. after eight months, his ... | 1992 | 1545912 |
| usage test of four endodontic sealers in macaca fascicularis monkeys. | root canals with vital pulps in four macaca fascicularis monkeys were subjected to pulp extirpation and root filling with gutta-percha and sealer, either ah26, endomethasone, kloroperka n-phi, or procosol. observation periods were 1 and 6 months. the results of treatment were evaluated by radiographic analysis (periapical index scores) and by histopathologic assessment of the remaining pulp and periapical tissues. sixty roots with acceptable termination (between 0.5 and 3 mm from apex) of the ro ... | 1992 | 1545966 |
| myeloma-like cast nephropathy caused by human recombinant soluble cd4 (scd4) in monkeys. | cd4 is the receptor for human immunodeficiency virus (hiv) on lymphocytes and macrophages. soluble cd4 (scd4), a recombinant truncated form of cd4, has been shown to inhibit hiv-1 in vitro and is being tested as a therapy for aids. preclinical studies in cynomolgus monkeys revealed a protein cast nephropathy after four daily intravenous doses of 100 mg/kg/day. renal lesions were not found in monkeys that received 10 mg/kg/day. the renal lesions consisted of proteinaceous tubular casts associated ... | 1992 | 1546739 |
| numbers and proportions of unmyelinated axons at cervical levels in the fasciculus gracilis of monkey and cat. | the present study is a quantitative analysis of the unmyelinated fiber population in the fasciculus gracilis of the second cervical segment of cat and monkey. we find that unmyelinated fibers represent 13.7% of the total fiber population in this pathway in the cat and 18.9% in the monkey (macaca fascicularis). the existence of such large numbers of these axons suggests that there may be a sizeable ascending fine primary afferent pathway in the fasciculus gracilis in cat and monkey whose destinat ... | 1992 | 1546808 |
| characterization of the levels of messenger ribonucleic acid that encode for luteinizing hormone receptor during the luteal phase of the primate menstrual cycle. | the present study was designed to characterize the expression of lh receptor messenger rna (mrna) in the primate corpus luteum throughout the luteal phase of the menstrual cycle. we obtained corpora lutea from cynomolgus monkeys at defined stages of the luteal phase. lh receptor mrna was demonstrated in monkey ovarian sections by in situ hybridization with a 35s-labeled antisense rna probe derived from rat lh receptor complimentary dna. the hybridization signals were confined to thecal layers of ... | 1992 | 1548340 |
| the effects of testosterone on lipids and eicosanoids in cynomolgus monkeys. | the effect of testosterone administration on plasma lipoproteins and eicosanoids was studied in 24 male cynomolgus monkeys. we hypothesized that elevated plasma testosterone would unfavorably alter plasma lipids as well as thromboxane a2 (txa2) and prostacyclin (pgi2), two eicosanoids that have been linked to the increased incidence of atherosclerosis, myocardial ischemia, and thrombosis. to test our hypothesis, half of the monkeys (n = 12) were subjected to 10 wk of testosterone treatment, wher ... | 1992 | 1549028 |
| effects of single and multiple inhalations of platelet-activating factor on airway cell composition and responsiveness in monkeys. | platelet-activating factor (paf) is a potent pro-inflammatory mediator that may play a role in the pathogenesis of airway hyper-responsiveness and asthma. in man, a single inhalation of paf induces a small but prolonged increase in airway responsiveness in some individuals. the purpose of this study was to determine the effects of single and multiple inhalations of paf on airway cell composition and responsiveness in monkeys. anaesthetized and intubated adult male cynomolgus monkeys were studied ... | 1992 | 1551034 |
| rapid presumptive identification and further characterization of bacteroides forsythus. | bacteroides forsythus is a fastidious anaerobic gram-negative organism associated with various forms of periodontal disease. it is dependent on n-acetylmuramic acid for growth. a method for rapid presumptive identification of human-derived strains of b. forsythus is presented, based on the following eight criteria: (i) positive activity for alpha-glucosidase, (ii) positive activity for beta-glucosidase, (iii) positive activity for sialidase, (iv) positive activity for trypsinlike enzyme, (v) neg ... | 1992 | 1551981 |
| renin inhibitors containing alpha-heteroatom amino acids as p2 residues. | a series of renin inhibitors having alpha-heteroatom amino acids as p2 substitutions has been prepared. examples where the heteroatom is oxygen, sulfur, or nitrogen are described. many of the compounds exhibit subnanomolar potency when tested in vitro against monkey renin. when selected compounds were tested orally in conscious, salt-depleted, normotensive, cynomolgus monkeys, low to moderate blood pressure lowering was observed. at an oral dose of 30 mg/kg, compound 53a lowered blood pressure b ... | 1992 | 1552498 |
| monkeys (macaca fascicularis) with rhinal cortex ablations succeed in object discrimination learning despite 24-hr intertrial intervals and fail at matching to sample despite double sample presentations. | six cynomolgus monkeys (macaca fascicularis) learned preoperatively a set of 10 concurrent object discriminations with 24-hr intertrial intervals. three then had the rhinal cortex removed bilaterally, whereas the other 3 remained as unoperated controls. the animals with ablations were impaired in reacquiring the preoperatively acquired set but subsequently learned without any impairment a new set of 10 discriminations that was presented in the same way. the monkeys with rhinal cortex ablations t ... | 1992 | 1554436 |
| neural-vascular relationships in central retina of macaque monkeys (macaca fascicularis). | the relationship of the vasculature to the neuronal layers was studied in whole-mounts and in sections of macaque retinas. like other central nervous structures, primate retinas have local variations in vascularity that reflect local variations in metabolism, rather than simply tissue thickness or volume. a special feature of the retina is a dense vascular plexus in the nerve fiber layer, which is unmyelinated and hence must generate a substantial metabolic demand for ion pumping. much of the re ... | 1992 | 1556592 |
| anti-idiotype monoclonal antibody elicits broadly neutralizing anti-gp120 antibodies in monkeys. | murine monoclonal antibodies (mabs) were raised against human, polyclonal, anti-gp120 antibodies (ab1) and were selected for binding to broadly neutralizing anti-gp120 antibodies in sera positive for human immunodeficiency virus (hiv). one anti-idiotype mab (ab2), 3c9, was found to be specific for human anti-gp120 antibodies directed against an epitope around the conserved cd4 attachment site of gp120. the 3c9 reactive human anti-gp120 antibodies (3c9+ ab) neutralized mn, iiib, rf, and four prim ... | 1992 | 1557358 |
| murine monoclonal anti-idiotope antibody breaks unresponsiveness and induces a specific antibody response to human melanoma-associated proteoglycan antigen in cynomolgus monkeys. | the mouse monoclonal antibody mem136 (mab1) is directed against an epitope on human melanoma-associated proteoglycan antigen (mpg). this epitope is also present on various normal human and subhuman tissues. a monoclonal murine anti-idiotope (anti-id) antibody (mab2), designated i-mel-2, was generated against mem136 and used as a surrogate antigen for the mpg molecule. i-mel-2 was tested in cynomolgus monkeys (macaca fascicularis) for its ability to induce anti-mpg humoral responses. all monkeys ... | 1992 | 1557374 |
| auxotrophic live oral shigella flexneri vaccine protects monkeys against challenge with s. flexneri of different serotypes. | the aromatic-dependent live shigella flexneri y strain sfl114, attenuated by a tn10-inactivated arod gene, was given as an oral vaccine to 14 macaca fascicularis monkeys. a significant clinical attenuation of sfl114 was seen (p = 0.0058) as all vaccinated monkeys tolerated 2 x 10(10)-1 x 10(11) bacteria of sfl114, whereas four out of seven monkeys orally given 1 x 10(11) of the virulent parent strain sfl1 developed shigellosis. the average excretion time for sfl114 and sfl1 were 2 and 18 days, r ... | 1992 | 1557932 |
| [histopathological study on the pars plicata of cynomolgus monkey by the cw nd:yag laser]. | the pars plicata of cynomolgus monkey eyes were coagulated by a continuous wave (cw) nd:yag laser contact system. the intraocular pressure and histopathological changes were observed during the following 3 months. the intraocular pressure decreased during the first week to a minimum level and returned gradually to the pre-coagulation level within 1-3 months. immediately after photocoagulation, both pigment and non-pigmented epithelium of pars plicata were destroyed. three weeks after the coagula ... | 1992 | 1558010 |
| antide bioavailability: single dose administration for suppression of testosterone and inhibin in male monkeys. | the pharmacokinetics and pharmacodynamics of a single sc injection of antide on testosterone (t) and inhibin secretion in intact male cynomolgus monkeys were examined. fifteen primates were randomized to three groups receiving: propylene glycol and water vehicle, 3 mg/kg antide, and 10 mg/kg of antide. antide at the 10 mg/kg dose caused long-term suppression of t ranging from 24-56 days. at the 3 mg/kg dose, suppression of t was of shorter duration. serum antide levels were significantly greater ... | 1992 | 1559337 |
| the effect of topical pgf2 alpha on uveoscleral outflow and outflow facility in the rabbit eye. | prostaglandin f2 alpha (pgf2 alpha) is a powerful ocular hypotensive agent in rabbit, cat, dog, monkey and human. in cynomolgus monkeys, the intraocular pressure (iop) lowering is due to increased uveoscleral outflow (fu). because the anatomy of the rabbit outflow apparatus differs significantly from that of the primate, we sought to determine whether the mechanism of the pgf2 alpha-induced iop fall was the same. pgf2 alpha tromethamine salt (pgf2 alpha-ts) (50 micrograms) applied to one eye of ... | 1992 | 1559555 |
| distribution and biological effects of inhaled 239pu(no3)4 in cynomolgus monkeys. | twenty male cynomolgus monkeys were exposed by inhalation either to an aerosol of 239pu(no3)4 to produce projected initial lung burdens of either 40, 10, or 4 kbq or to a carrier aerosol as a control. animals died or were sacrificed at 0.01, 1, 3, 6, 12, 24, 40, and 99 months after inhalation, and the distribution and biological effects of the 239pu were determined. the 239pu cleared efficiently from the lungs so that less than 0.05 kbq remained at 99 months after exposure to 40 kbq. total skele ... | 1992 | 1561321 |
| hexachlorobenzene (hcb) suppresses circulating progesterone concentrations during the luteal phase in the cynomolgus monkey. | hexachlorobenzene (hcb) is a known reproductive toxin. however, the full spectrum of its reproductive toxicity is unknown. consequently, the effect of hcb on serum oestradiol (e2) and progesterone (p4) concentrations during the follicular (days 1-9), periovulatory (days 10-14) and luteal (days 15 to beginning of next menses) phases was investigated in the spontaneously cycling cynomolgus monkey. adult female cynomolgus monkeys (n = 16) were randomly assigned to one of four treatment groups and o ... | 1992 | 1564247 |
| [the effect of a high-intensity radiation exposure on the brain function of monkeys. the postradiation changes in the eeg response to rhythmic photostimulation]. | in experiments with monkeys (macaca fascicularis) it has been shown that whole-body irradiation with a dose of 45 gy (6.5 gy/s) causes considerable changes in the eeg response to rhythmic photostimulation (ps). these changes are: reduction of the desynchronizing effect of ps with regard to a background rhythmicity; decrease in the reception rate of the rhythms of light flashes (rlf); narrowing of the rlf frequency range; and increase in the reaction momentum. the postirradiation changes in the e ... | 1992 | 1565780 |
| acid reaction products of indole-3-carbinol and their effects on cytochrome p450 and phase ii enzymes in rat and monkey hepatocytes. | the effects of three acid condensation products of indole-3-carbinol (i3c), i.e. 3,3'-diindolylmethane (dim), 5,6,11,12,17,18-hexahydrocyclonona[1,2-b:4,5-b':7,8-b"]tri-indole (cti) and 2,3-bis[3-indolylmethyl]indole (bii), on cytochrome p450 and phase ii enzymes were studied in primary cultures of rat and cynomolgus monkey liver cells. in rat hepatocytes all three indole derivatives dose-relatedly induced the ethoxyresorufin o-dealkylation (erod) activity (to 24-fold) and 7 alpha-hydroxylation ... | 1992 | 1567468 |
| infection of owl monkeys (aotus trivirgatus) and cynomolgus monkeys (macaca fascicularis) with hepatitis e virus from mexico. | owl and cynomolgus monkeys were inoculated with hepatitis e virus (hev) to compare disease models and produce antibody and virus. by immune electron microscopy (iem), all six owl monkeys were shown to have serologic responses manifested by unusually high levels of anti-hev at 6 months, but only three developed hepatitis. virus-related antigen in liver (hev ag) was detected by immunofluorescence microscopy of biopsies from two of four owl monkeys; one with hev ag also had hev in acute-phase bile ... | 1992 | 1569334 |
| malignant lymphoma associated with human aids and with siv-induced immunodeficiency in macaques. | malignant lymphomas associated with human (hiv) and simian (siv) immunodeficiency virus infections are reviewed and compared. recent observation of a high frequency of lymphomas in a series of cynomolgus macaques, highly immunodeficient after infection with sivsm(smm3) are described. in addition to the increased frequency in human and monkey aids, siv and hiv lymphomas share several important features. clinically and by histology they present as aggressive high-grade malignant tumors with a pred ... | 1992 | 1571194 |
| the onset and recovery from airway hyperresponsiveness: relationship with inflammatory cell infiltrates and release of cytotoxic granule proteins. | previous studies from our laboratory have demonstrated a temporal relationship between eosinophil influx into the airways and the onset of airway hyperresponsiveness to inhaled methacholine. the purpose of the present study was to extend this observation by evaluating changes in airway cellular composition and measuring the levels of granulocyte-derived mediators recovered in bal fluid during the onset and recovery from antigen-induced airway hyperresponsiveness. airway cellular composition, air ... | 1992 | 1571822 |
| enzyme immunosorbent assay for ebola virus antigens in tissues of infected primates. | a sandwich enzyme immunosorbent assay (eia) using a mixture of mouse monoclonal antibodies for antigen capture and polyclonal hyperimmune rabbit anti-ebola virus serum for antigen detection was developed and evaluated on the tissues of monkeys naturally or experimentally infected with strains of ebola viruses. when compared with virus isolation, the antigen detection eia was both sensitive and specific: 44 of 45 (97.7%) liver homogenates and 38 of 41 (92.7%) spleen homogenates that were culture ... | 1992 | 1572982 |
| activity in monkey substantia nigra neurons related to a simple learned movement. | single cell activity was recorded in the pars compacta (snc) and pars reticulata (snr) of the substantia nigra (sn) in 4 unanesthetized macaca fascicularis to determine the motor role of the nucleus. animals were trained to perform a simple task that involved moving a lever by elbow flexion-extensions, in the horizontal plane using the hand contralateral to the recording site. two monkeys learnt to execute the task on both sides. electromyograms (emg) of limb muscles were recorded simultaneously ... | 1992 | 1577102 |
| immunoglobulin ch gene family in hominoids and its evolutionary history. | the organization of the human immunoglobulin ch gene suggests that a gene duplication involving the c gamma-c gamma-c epsilon-c alpha region has occurred during evolution. we previously showed that both chimpanzee and gorilla have two 5'-c epsilon-c alpha-3', as in human, and that orangutan, gibbon, and old world monkeys have one c epsilon gene and one, two, and one c alpha gene(s), respectively. in addition to these clustered ch genes, there is one processed c epsilon pseudogene in each species ... | 1992 | 1577482 |
| localization of corticotropin-releasing factor-like immunoreactivity in monkey olfactory bulb and secondary olfactory areas. | electrophysiological and anatomical observations suggest that terminals of olfactory bulb mitral cells ending in rat primary olfactory cortex exert certain postsynaptic effects via an excitatory amino acid neurotransmitter. recent anatomical studies have shown that several peptides, most notably corticotropin-releasing factor (crf) (imaki et al., '89) brain res., 496: 35-44), are also localized within rat olfactory bulb projection neurons, thus raising the possibility that there is a peptide cot ... | 1992 | 1577989 |
| functional properties of single neurons in the face primary motor cortex of the primate. i. input and output features of tongue motor cortex. | 1. we have recently demonstrated that reversible, cooling-induced inactivation of the face motor cortex results in a severe impairment in the ability of monkeys (macaca fascicularis) to perform a tongue-protrusion task but produces only relatively minor effects on the performance of a biting task by the same monkeys. to establish a neuronal correlate for these different behavioral relations, the present study has detailed the afferent input and intracortical microstimulation (icms)-defined outpu ... | 1992 | 1578252 |
| primary afferent interactions: analysis of calcitonin gene-related peptide-immunoreactive terminals in contact with unlabeled and gaba-immunoreactive profiles in the monkey dorsal horn. | the present study analyses the relationship of calcitonin gene-related peptide (cgrp)-immunoreactive primary afferent terminals with unlabeled and gaba-immunoreactive profiles in the primate (macaca fascicularis) dorsal horn. one-hundred cgrp-immunoreactive terminals located in the superficial dorsal horn were quantitatively analysed and all profiles in apposition or in synaptic contact with these terminals were categorized as either axon terminals or dendrites with or without vesicles. these pr ... | 1992 | 1579216 |
| the use of thalamotomy in the treatment of levodopa-induced dyskinesia. | peak dose dyskinesia is a major problem in the treatment of parkinsonian patients with levodopa and yet this remains the best pharmacological agent for treating the condition. the hypothesis which this research set out to test was that thalamotomy in the area of the thalamus which receives the input from the medial segment of the globus pallidus would decrease or prevent the dyskinesia. a well established primate model of parkinsonism was used. eight monkeys (macaca fascicularis) were rendered p ... | 1992 | 1580197 |
| effects of topical ethacrynic acid adducts on intraocular pressure in rabbits and monkeys. | we evaluated the effect of topical ethacrynic acid on rabbit and monkey intraocular pressure. in a preliminary experiment, 100-mmol/l ethacrynic acid applied topically to dutch-belted rabbit eyes was associated with an 8-mm hg lowering of intraocular pressure. however, corneal edema was severe, and the corneal epithelium sloughed off. to try to maintain the pressure-lowering effect but reduce the corneal side effects, we attempted to create an adduct of ethacrynic acid by utilizing ethacrynic ac ... | 1992 | 1580849 |
| polymyxin b-induced bronchial neutrophilia does not alter airway responsiveness to methacholine in cynomolgus monkeys. | the purpose of this study was to develop a primate model of chronic bronchial neutrophilia to investigate the role of neutrophils in the pathogenesis of airway hyperresponsiveness. ten adult male cynomolgus monkeys (macaca fascicularis) were anaesthetized and intubated for each study. six animals each received a total of seven inhalation treatments with polymyxin b (200 micrograms) over a 24 day period. four control animals received an identical treatment regime with vehicle inhalations. airway ... | 1992 | 1586875 |
| c-terminal modifications of nonpeptide renin inhibitors: improved oral bioavailability via modification of physicochemical properties. | we describe the development of a series of soluble, potent, and bioavailable nonpeptide renin inhibitors. these inhibitors derived from a series of novel nonpeptide renin inhibitors which were recently identified in our laboratories, by alteration of the nature of the c-terminus (p2') of the molecules. introduction of basic substituents into modified hydroxyethylene dipeptide isosteres gave inhibitors with improved solubility as well as improved potency against human plasma renin. in addition, t ... | 1992 | 1588555 |
| hepatitis e virus (hev): strain variation in the nonstructural gene region encoding consensus motifs for an rna-dependent rna polymerase and an atp/gtp binding site. | hepatitis is transmitted by a number of infectious agents. the epidemiological characterization of waterborne or enterically transmitted non-a, non-b hepatitis (et-nanbh) is unique when compared with other known hepatitides. we have reported on the molecular cloning of a cdna clone derived from the etiologic agent associated with et-nanbh, the hepatitis e virus (hev). the complete sequence of these first molecular clones, isolated from an hev-infected human after passage in macaca fascicularis ( ... | 1992 | 1589964 |
| regional differences in arterial low density lipoprotein metabolism in surgically postmenopausal cynomolgus monkeys. effects of estrogen and progesterone replacement therapy. | to determine if arterial lipoprotein metabolism may be involved in mediating well-known anatomic regional differences in susceptibility to atherosclerosis, arterial low density lipoprotein (ldl) metabolism and extent of atherosclerosis were studied in 17 ovariectomized female cynomolgus monkeys. the animals were fed an atherogenic diet for 18 weeks, during which time one group received 17 beta-estradiol and cyclic progesterone treatment (n = 9) and the controls received no hormone replacement th ... | 1992 | 1591231 |
| the role of cholesteryl ester transfer protein in primate apolipoprotein a-i metabolism. insights from studies with transgenic mice. | to assess the effects of cholesteryl ester transfer protein (cetp) on the primate lipoprotein profile, a transgenic mouse expressing cynomolgus monkey cetp was developed. the c57bl/6 mouse was used, and four lines expressing the primate cetp were established. the level of cetp activity in the plasma of the transgenic mice ranged from values similar to those obtained for the monkey to levels approximately sixfold higher than that in the normal monkey. when all of the lines were taken into conside ... | 1992 | 1591233 |
| reproductive toxicity of chronic lead exposure in the female cynomolgus monkey. | the effect of chronic lead (pb) exposure on menstrual function and circulating concentrations of luteinizing hormone (lh), follicle stimulating hormone (fsh), estradiol (e2) and progesterone (p4) were studied in female nulliparous cynomolgus monkeys (n = 32). pb acetate (1500 micrograms/kg bw/day) was administered by capsule to monkeys in teh following groups; exposure from birth to 10 years (lifetime, n = 8), postnatal day 300 to 10 years (adolescent, n = 8), and postnatal days 0 to 400 (childh ... | 1992 | 1591470 |
| antitumor effects of b3-pe and b3-lyspe40 in a nude mouse model of human breast cancer and the evaluation of b3-pe toxicity in monkeys. | b3 is a tumor-reactive monoclonal antibody (mab) that binds to a limited number of normal tissues. immunotoxins made with b3 coupled to either pseudomonas exotoxin (pe) or recombinant forms of pe with a deletion of the cell-binding domain (lyspe40) have been shown to cause complete tumor regression in nude mice bearing a rapidly growing a431 (l. h. pai et al., proc. natl. acad. sci. usa, 88: 3358-3362, 1991) human epidermoid carcinoma. in this study we show that an immunotoxin composed of mab b3 ... | 1992 | 1591729 |
| fibrinous reaction on implanted intraocular lenses. a comparison of conventional pmma and heparin surface modified lenses. | we studied the fibrinous reaction after intraocular lens (iol) implantation in the posterior chamber of cynomolgus monkeys. in 50% of the eyes, we implanted an iol made of conventional poly-(methyl methacrylate) (pmma); in the remaining eyes we implanted a pmma iol with a heparin modified surface. two, 4, 8, and 18 weeks after surgery the eyes were examined by slitlamp for fibrinous reactions on and around the iol surface. at weeks 4, 8, and 18 there was a marked decrease in fibrinous reaction i ... | 1992 | 1593429 |
| a comparison of total body water measurements using whole-body magnetic resonance imaging versus tritium dilution in primates. | standard techniques conventionally used to assess body composition have various drawbacks which include a requirement for specialized equipment and expertise not widely available, radiation exposure, extensive study time periods, and limited clinical utility. anthropometric methods, which are more clinically feasible, also involve substantially greater calculation error. this study was designed to compare a newly developed whole-body magnetic resonance (mr) imaging technique with standard tritiu ... | 1992 | 1593876 |
| taste neurons in the cortex of the alert cynomolgus monkey. | the activity of single neurons in the gustatory cortex of alert cynomolgus monkeys was analyzed. taste-evoked activity in response to the four prototypical taste stimuli was recorded from a cortical gustatory area comprising the frontal operculum and adjoining anterior insula. spontaneous activity for 364 gustatory neurons was 3.9 +/- 4.9 (mean +/- sd) spikes/s. mean net (gross minus spontaneous) discharge rates for all gustatory neurons were: 1.0 m glucose = 4.9 +/- 11.6, 0.3 m nacl = 3.2 +/- 7 ... | 1992 | 1596754 |
| peripheral and not central suppression of ovarian function during osmotic pump infusion of adrenocorticotropin-(1-24) for one menstrual cycle in the cynomolgus monkey and its partial compensation by a transitory elevation of sex hormone-binding globulin levels. | the purpose of our study was to assess the impact of subchronic administration of acth-(1-24) on ovarian function in the primate using a repeated measures experimental design. osmotic pumps that released acth-(1-24) at a dose of 67 micrograms/day were implanted sc in four cynomolgus monkeys for one menstrual cycle. the pumps were filled with saline for the two control cycles, one of which preceded and one of which followed peptide infusion. administration of acth-(1-24) elevated cortisol levels ... | 1992 | 1597155 |
| the source of sensory fibres of the inferior conjunctiva of monkeys. | dissection, light and electron microscopy combined with degeneration induced by intracranial nerve lesions and superficial nerve transection were used to trace the sources of sensory fibres of the inferior conjunctiva of cynomolgus monkeys. most nerves destined for the inferior conjunctiva were branches of the lacrimal nerve and entered the inferior eyelid laterally. following unilateral ophthalmic neurotomy in three monkeys and combined ophthalmic/maxillary neurotomy in one monkey, substantial ... | 1992 | 1597293 |
| association of ebola-related reston virus particles and antigen with tissue lesions of monkeys imported to the united states. | during 1989-1990, an epizootic involving a filovirus closely related to ebola virus occurred in a reston, virginia, primate-holding facility. tissues were collected from cynomolgus monkeys and examined by electron microscopy and immunohistochemistry for ebola-related viral antigen. viral replication was extensive in fixed tissue macrophages, interstitial fibroblasts of many organs, circulating macrophages and monocytes, and was observed less frequently in vascular endothelial cells, hepatocytes, ... | 1992 | 1597531 |
| stabilization of gaze during circular locomotion in light. i. compensatory head and eye nystagmus in the running monkey. | 1. a rhesus and cynomolgus monkey were trained to run around the perimeter of a circular platform in light. we call this "circular locomotion" because forward motion had an angular component. head and body velocity in space were recorded with angular rate sensors and eye movements with electrooculography (eog). from these measurements we derived signals related to the angular velocity of the eyes in the head (eh), of the head on the body (hb), of gaze on the body (gb), of the body in space (bs), ... | 1992 | 1597704 |
| susceptibility of macaca fascicularis monkeys from mauritius to different species of plasmodium. | macaca fascicularis monkeys from mauritius were shown to be susceptible via sporozoite inoculation to 7 species of plasmodium (p. fragile, p. coatneyi, p. gonderi, p. inui, p. cynomolgi, p. knowlesi, and p. fieldi), indigenous to macaques in southeastern asia. four monkeys were sequentially infected with different species of plasmodium to determine maximum and course of parasitemia. in 2 nonsplenectomized monkeys, p. fragile developed maximum parasite counts of only 134 and 155/microliters. for ... | 1992 | 1597796 |
| [the effect of ethanol on the metabolism of methamphetamine]. | in order to clarify the effects of ethanol (etoh) on metabolism of methamphetamine hydrochloride (ma-hcl), the plasma and urine samples were taken from three groups of three male crab-eating monkeys (macaca fascicularis). ma-hcl was given to the first group, both ma-hcl and etoh were given once to the second group, the after consecutive administration of etoh for four weeks, both ma-hcl and etoh were given to the third group. comparisons were made of the sequential change in the concentration of ... | 1992 | 1597931 |
| live oral auxotrophic shigella flexneri sfl124 vaccine with a deleted arod gene: characterization and monkey protection studies. | shigella flexneri sfl124, with a deletion encompassing all, or nearly all, of the coding sequence of gene arod was obtained after selection on a fusaric acid medium supplemented with 2,3-dihydroxybenzoic acid for tetracycline-sensitive mutants of s. flexneri sfl114 which is an arod::tn10 transductant. two of 20 tetracycline-sensitive mutants tested in colony hybridization with a 32p-labelled dna probe of approximately 1400 base pairs (comprising all except the 75 n-terminal base pairs of the cod ... | 1992 | 1598787 |
| effects of a low-fat diet on brain serotonergic responsivity in cynomolgus monkeys. | 1992 | 1599991 | |
| in vivo distribution of [11c]-busulfan in cynomolgus monkey and in the brain of a human patient. | the in vivo distribution of the antileukemic agent busulfan labeled with the positron-emitting radionuclide carbon 11 was investigated in cynomolgus monkeys and in a human patient using positron emission tomography. after i.v. injection of the radiotracer, its regional uptake was monitored for about 1 h in the monkey's body and, in a separate experiment, in the monkey's brain. the concentration of radioactivity in the liver, which showed the highest levels of all the organs scanned, increased th ... | 1992 | 1600599 |
| high-performance liquid chromatographic procedure for specificity testing of radioimmunoassays: rolipram. | a high-performance liquid chromatographic (hplc) procedure for testing the specificity of radioimmunoassays (ria) was developed using the same method of extraction as in the ria, followed by fractionation of the extract by hplc and subsequent measurement of cross-reactions in all the fractions according to the normal ria procedure. the ria of rolipram, an antidepressant drug, was checked in plasma samples obtained from pharmacokinetic studies in rats, rabbits, cynomolgus monkeys and humans. the ... | 1992 | 1601964 |
| establishment and characterization of macaca fascicularis lymphoblastoid cell lines. | a panel of cynomolgus macaque lymphoblastoid cell lines (lcl) was established by transforming peripheral blood mononuclear cells (pbmc) with herpesvirus papio (hvp), and selected lines were examined by flow cytometry. results indicate that hvp-transformed macaque lcl are phenotypically heterogeneous and resemble human epstein-barr virus (ebv)-transformed lcl in the abundant expression of major histocompatibility complex (mhc) class i and class ii molecules. at least some lines are of b cell orig ... | 1992 | 1602455 |
| a prospective analysis of endometrial cycle changes by ultrasound in the female cynomolgus monkey. | endometrial cycle changes in adule female cynomolgus monkeys with normal ovulatory cycles were examined prospectively by real-time ultrasound. endometrial thickness, as measured by ultrasound, was correlated with cycle day and serum estradiol (e2) and progesterone (p4) levels. we conclude that ultrasound is a reliable method of diagnosis of endometrial cycle stage. | 1992 | 1602457 |
| neuroactive amino acids in the area postrema. an immunocytochemical investigation in rat with some observations in cat and monkey (macaca fascicularis). | the localization of five neuroactive amino acids in the rat area postrema was studied by postembedding immunocytochemistry in semithin and ultrathin sections. antisera to gaba, glycine, glutamate and aspartate produced labelling of cells that were identified as neurons in the electron microscope. gaba-like and glycine-like immunoreactivities occurred in about 20% and 60% of the neurons, respectively, and a minor proportion of the cells displayed both immunoreactivities, suggesting a cellular col ... | 1992 | 1605365 |
| localization of a carbohydrate antigen associated with growing oocytes and ovarian surface epithelium. | we used a monoclonal antibody (ps1) to a carbohydrate antigen to study the development of the oocyte and follicle during early stages of differentiation in several mammalian species. this antigen has been shown to localize within the cytoplasm of oocytes in primordial follicles as well as in growing oocytes. it is also localized within distinct layers of the zona pellucida (zp) of developing follicles. although this antibody was made against a specific zp glycoprotein, the antigen also appears t ... | 1992 | 1607636 |
| developmental changes in the uptake of testosterone by the primate brain. | during the neonatal period in male macaques, the testis produces adult-like levels of plasma testosterone (t), but the function of this in development is not understood. to investigate the interaction of t with the neonatal brain, 4 male and 5 female cynomolgus monkeys were gonadectomized 2-5 days after birth, and were injected subcutaneously 3 days later with 500 microci [3h]-testosterone ([3h]-t). 60 min later, brains and other tissue samples were removed. purified nuclear pellets were prepare ... | 1992 | 1608511 |
| metabolism of [14c]benzene by cynomolgus monkeys and chimpanzees. | rodent bioassays indicate that b6c3f1 mice are more sensitive to the carcinogenicity of benzene than are rats. the urinary profile of benzene metabolites is different in rats vs mice. mice produce higher proportions of hydroquinone conjugates and muconic acid, indicators of metabolism via pathways leading to putative toxic metabolites, than do rats. in both species, metabolism to hydroquinone and muconic acid is favored at low concentrations of benzene, indicating that these pathways are easily ... | 1992 | 1609420 |
| neurovirulence tests of type 3 oral poliovirus vaccine manufactured by lederle laboratories, 1964-1988. | oral poliovirus vaccine (opv) is tested for safety by evaluation of neurovirulence in rhesus and cynomolgus macaques. after intraspinal or intrathalamic injection of varying doses of vaccine, monkeys are followed for 17-21 days, killed, and a histopathological evaluation is made of the severity of poliomyelitis lesions in the spinal cord and brainstem. each production lot of vaccine is compared with a type 1 opv reference virus tested by the same method. records of neurovirulence tests on produc ... | 1992 | 1609550 |
| nucleotide sequence of the cynomolgus monkey apolipoprotein a-i gene and corresponding flanking regions. | the cynomolgus monkey (macaca fascicularis) apolipoprotein a-i (apo a-i) gene and corresponding flanking regions have been isolated from a cynomolgus monkey genomic library and completely sequenced. comparison with the human sequence shows a greater than 90% homology with the monkey sequence, overall. the monkey apo a-i structural gene (1856 bp) is representative of the general apolipoprotein gene structure and consists of four exons and three introns. minor differences exist between the monkey ... | 1992 | 1610902 |
| transplantation of embryonic serotonin immunoreactive neurons into the transected spinal cord of adult monkey (macaca fascicularis). | five adult monkeys (macaca fascicularis) underwent a total section of the spinal cord at the thoracic level (t6). four of them received a daily treatment with cyclosporin (10 mg/kg). ten days later, two animals treated with cyclosporin and one without cyclosporin received at t8 and t10 levels an injection of a cell suspension prepared from the rhombencephalon of a 40-day-old macaque embryo. two control animals received one injection of hank's balanced salt solution. the animals were sacrificed a ... | 1992 | 1611532 |
| exogenous gonadotrophin-releasing hormone (gnrh) stimulates lh secretion in male monkeys (macaca fascicularis) treated chronically with high doses of a gnrh antagonist. | we reported previously that after a single injection of a gonadotrophin-releasing hormone (gnrh) antagonist to male monkeys, exogenous gnrh stimulated lh secretion in a time- and dose-dependent manner, indicating that gnrh antagonist-induced blockade of lh secretion resulted from pituitary gnrh receptor occupancy. the present study was performed to investigate whether gnrh can also restore a blockade of lh and testosterone secretion during chronic gnrh antagonist administration. four adult male ... | 1992 | 1613445 |
| infection of cynomolgus monkeys with a chimeric hiv-1/sivmac virus that expresses the hiv-1 envelope glycoproteins. | replication competent chimeric viruses that express the gag and pol proteins of sivmac and the env proteins of hiv-1 were made. one such chimeric virus, shiv-4, that expresses the vif, vpx, vpr, and nef regulatory genes of siv and the tat and rev regulatory genes of hiv-1 replicated efficiently in cynomolgus monkeys. this model system can be used to evaluate the efficacy of anti-hiv-1 vaccines directed at the envelope glycoproteins, anti-hiv-1 envelope glycoprotein antiserum or monoclonal antibo ... | 1992 | 1613662 |
| the interaction of testosterone with the brain of the orchidectomized primate fetus. | at certain times during gestation, the testes of the fetal macaque produce plasma levels of testosterone (t) that are similar to those of adults. it is thought that testosterone acts on the brain via estrogen and androgen receptors to organize the development of sexually dimorphic neural structures that underlie sex differences in behavior. to test the proposition that there are male-female differences in the occupation of steroid receptor binding sites during fetal development in the cynomolgus ... | 1992 | 1617431 |