Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| accumulation of some heavy metals in hysterothylacium aduncum (nematoda) and its host sea bream, sparus aurata (sparidae) from north-eastern mediterranean sea (iskenderun bay). | the sea bream's nematode and sparus aurata, sampled from the iskenderun bay, mediterranean, in march 2008 were analyzed by inductively coupled plasma-atomic emission spectrometry for their some heavy metal (cd, cr, cu, fe, hg, mn, mg, pb, and zn) levels. the metal concentrations of the parasites were compared to different organs (liver, muscle, gill, intestine, and skin) of the fish hosts. there were significant differences in cd, cr, cu, fe, mn, zn, hg, mg, and pb concentrations in tissues of f ... | 2011 | 20422284 |
| effect of ivermectin on the liver of gilthead sea bream sparus aurata: a proteomic approach. | gilthead sea bream sparus aurata is the most commercialized mediterranean aquacultured fish species. ivermectin has recently (experimentally) started to be used to control ectoparasitic infestations in mediterranean cultured marine fish. the potential hepatotoxicity of ivermectin was investigated in gilthead sea bream juveniles (35g) following oral administration at the recommended dose of 0.2 mgkg(-1) fish for 10d. difference gel electrophoresis technology (dige) was used to study the effect of ... | 2010 | 20451238 |
| identification of a novel transforming growth factor-beta (tgf-beta6) gene in fish: regulation in skeletal muscle by nutritional state. | the transforming growth factor-beta (tgf-beta) family constitutes of dimeric proteins that regulate the growth, differentiation and metabolism of many cell types, including that of skeletal muscle in mammals. the potential role of tgf-betas in fish muscle growth is not known. | 2010 | 20459854 |
| phylogenetic consistencies among chondrichthyan and teleost fishes in their bioaccumulation of multiple trace elements from seawater. | multi-tracer experiments determined the accumulation from seawater of selected radioactive trace elements (mn-54, co-60, zn-65, cs-134, am-241, cd-109, ag-110m, se-75 and cr-51) by three teleost and three chondrichthyan fish species to test the hypothesis that these phylogenetic groups have different bioaccumulation characteristics, based on previously established contrasts between the carcharhiniform chondrichthyan scyliorhinus canicula (dogfish) and the pleuronectiform teleost psetta maxima (t ... | 2010 | 20471062 |
| [assessment of seawater quality along the asinara gulf. note 1: preliminary results of seawater monitoring using dicentrarchus labrax and sparus aurata as bioindicators]. | in recent years, physical-chemical, chemical and microbiological testing systems to water's control was matched by the use of "biomarkers" such as algae, nematodes, anellidi, porifera, molluscs and arthropods (crustaceans), although these are phylogenetically distant from humans and they differ in methods of recruitment, toxico-kinetics and metabolism of xenobiotics. that is why today the predatory fishes (tuna, mackerel, sea bream, sea bass and swordfish) are among the most widely used in biomo ... | 2010 | 20476660 |
| regulatory regions in the promoter and first intron of sparus aurata growth hormone gene: repression of gene activity by a polymorphic minisatellite. | transcriptional activity of the gilthead sea bream (sparus aurata) growth hormone (sagh) gene promoter has been investigated using transient transfection assays in gh3 cells. analysis of two fragments (1.7 kb and a 5'-deleted 0.9 kb) of sagh gene promoter directed luciferase reporter gene activity, indicating transcriptional activity of both fragments in vitro. the shorter fragment, containing five potential binding sites for the pituitary-specific transcription factor ghf-1/pit-1, two camp-resp ... | 2008 | 20483206 |
| characterization and functional analysis of the 5' flanking region of myosin light chain-2 gene expressed in white muscle of the gilthead sea bream (sparus aurata). | the promoter region ( approximately 1400 bp) of myosin light chain 2 gene of fast skeletal muscle from the marine fish sparus aurata was cloned, sequenced and characterized. it contains a consensus sequence for tata box, six perfect e-boxes known as binding sites to myogenic basic helix-loop-helix transcription factors and four putative mef2-binding sites. three genomic fragments (truncated at their upstream region) of 244, 650 and 1400 bp showed promoter activity evidenced by muscle-specific re ... | 2007 | 20483292 |
| a new genotype of lymphocystivirus isolated from cultured gilthead seabream, sparus aurata l., and senegalese sole, solea senegalensis (kaup). | 2010 | 20487141 | |
| correlated expression profile of extracellular matrix-related molecules during the inflammatory response of the teleost fish gilthead seabream. | extracellular matrix (ecm) components, in addition to their structural functions, interact with cell surface receptors and intracellular components to modulate the transduction of signals for cell growth, differentiation, migration, proliferation, polarization, apoptosis and inflammation. our previous findings in the gilthead seabream (sparus aurata l.), a marine seasonal hermaphrodite teleost fish, have shown that both endocrine and immune stimuli modulate the expression of matrix metalloprotea ... | 2010 | 20488200 |
| metabolic and molecular stress responses of gilthead seam bream sparus aurata during exposure to low ambient temperature: an analysis of mechanisms underlying the winter syndrome. | the winter syndrome in the gilthead sea bream sparus aurata indicates that the species is exposed to critically low temperatures in mediterranean aquaculture in winter. the present study of metabolic patterns and molecular stress responses during cold exposure was carried out to investigate this "disease", in light of the recent concept of oxygen and capacity limited thermal tolerance. the metabolic profile of fuel oxidation was examined by determining the activities of the enzymes hexokinase (h ... | 2010 | 20514487 |
| transactivation of cytosolic alanine aminotransferase gene promoter by p300 and c-myb. | alanine aminotransferase (alt) provides a molecular link between carbohydrate and amino acid metabolism. in the cell context, the predominant alt isozyme is located in the cytosol. to gain insight into the transcriptional regulation of the cytosolic alt gene (calt), we cloned and characterized the calt promoter from gilthead sea bream (sparus aurata). transient transfection of sea bass larvae cells with deleted calt promoter constructs and electrophoretic mobility shift assays allowed us to iden ... | 2010 | 20573775 |
| ontogeny of osmoregulation and salinity tolerance in the gilthead sea bream sparus aurata. | the gilthead sea bream, sparus aurata, is a euryhaline teleost that hatches in the open sea. the larvae drift to the coast and juveniles migrate into estuaries and lagoons where the salinity of the water may vary from brackish to hyper-saline. the ontogeny of osmoregulation in sparus aurata was studied at successive stages, from day 1 (d1) post-hatch to the late juvenile stage (d300) after exposure to different salinities ranging from fresh water to 45.1 per thousand, at 18 degrees c. survival r ... | 2010 | 20601051 |
| molecular characterization of a gilthead sea bream (sparus aurata) muscle tissue cdna for carnitine palmitoyltransferase 1b (cpt1b). | understanding the control of piscine fatty acid metabolism is important for determining the nutritional requirements of fish, and hence for the production of optimal aquaculture diets. the regulation and expression of carnitine palmitoyltransferase 1 (cpt1; ec no 2.3.1.21) are critical processes in the control of fatty acid metabolism, and here we report a cdna from gilthead sea bream (sparus aurata) which encodes a protein with high identity to vertebrate cpt1. this sea bream cpt1 mrna is predo ... | 2010 | 20601065 |
| carbohydrate patterns in the digestive tract of sparus aurata l. and psetta maxima (l.) (teleostei) parasitized by enteromyxum leei and e. scophthalmi (myxozoa). | the influence of enteromyxum spp. infections on the carbohydrate patterns of the digestive tract of gilthead sea bream (gsb) sparus aurata l. and turbot (tb) psetta maxima (l.) has been studied. histochemical stainings to differentiate the types of mucins and lectin-binding assays to detect terminal carbohydrate residues were applied to histological sections of gsb and tb uninfected or infected by enteromyxum leei and e. scophthalmi, respectively. the number of intestinal gc decreased in severel ... | 2010 | 20601108 |
| absence of anisakid larvae in farmed european sea bass (dicentrarchus labrax l.) and gilthead sea bream (sparus aurata l.) in southeast spain. | in the present study, a total of 871 farmed fish, 612 gilthead sea bream (sparus aurata l.) and 259 european sea bass (dicentrarchux labrax l.), were examined for the presence of anisakid larvae. two diagnostic methods were applied, visual inspection and artificial digestion based on the degradation of fish soft tissue in an acidified pepsin enzyme solution. none of the samples examined in this study contained any anisakid parasite. the results suggest that consumption of these farmed fish speci ... | 2010 | 20615348 |
| growth/differentiation factor-11: an evolutionary conserved growth factor in vertebrates. | growth and differentiation factor-11 (gdf-11) is a member of the transforming growth factor-β superfamily and is thought to be derived together with myostatin (known also as gdf-8) from an ancestral gene. in the present study, we report the isolation and characterization of gdf-11 homolog from a marine teleost, the gilthead sea bream sparus aurata, and show that this growth factor is highly conserved throughout vertebrates. using bioinformatics, we identified gdf-11 in tetraodon, takifugu, medak ... | 2010 | 20694476 |
| establishment of dominance relationships in gilthead sea bream sparus aurata juveniles during feeding: effects on feeding behaviour, feed utilization and fish health. | in gilthead sea bream sparus aurata held in groups of two, five or 10 fish, social hierarchies were observed. subordinate s. aurata were characterized by elevation of basal levels of plasma cortisol, together with a reduced immunological potential. subordinate fish also showed lower feed intake, feed utilization and lower growth. fatty acid composition was also affected by social status, with a lower content of saturated acids, oleic and eicosapentaenoic, in muscle and liver of fish considered a ... | 2009 | 20735600 |
| melatonin concentrations during larval and postlarval development of gilthead sea bream sparus auratus: more than a time-keeping molecule? | in this study, melatonin (mel) and thyroxine (t(4)) concentrations were measured during larval and postlarval development of gilthead sea bream sparus auratus hormones were measured in whole bodies of larvae or the head and trunk of postlarvae after 67 days of exposure to constant light, 24l:0d, constant darkness, 0l:24d or 12l:12d and in the plasma of 6 month juveniles kept under the 12l:12d, 0l:24d and 24l:0d regimes. high mel concentrations in larvae suggested a distinct role of mel in early ... | 2009 | 20738488 |
| older triploid fish retain impaired reproductive endocrinology in the european sea bass dicentrarchus labrax. | this paper reports on an evaluation of growth, gonadal development and reproductive endocrinology of older triploid (3n) european sea bass dicentrarchus labrax in comparison with their diploid (2n) counterparts throughout their fifth and seventh annual cycle of life. while older triploids retained impaired reproductive endocrinology, a sexually related dimorphic growth was observed with 3n females attaining the largest sizes. comparisons of some body indexes showed that 3n females had a signific ... | 2009 | 20738514 |
| replacement of dietary fish oil by vegetable oils affects humoral immunity and expression of pro-inflammatory cytokines genes in gilthead sea bream sparus aurata. | commercial gilthead sea bream feeds are highly energetic, fish oil traditionally being the main lipid source. but the decreased fish oil production together with the increased prices of this oil encourages its substitution by vegetable oils, imposing new nutritional habits to aquaculture species. partial replacement of fish oil by vegetable oils in diets for marine species allows good feed utilization and growth but may affect fish health, since imbalances in dietary fatty acids may alter fish i ... | 2010 | 20817101 |
| prolactin-induced production of reactive oxygen species and il-1β in leukocytes from the bony fish gilthead seabream involves jak/stat and nf-κb signaling pathways. | prolactin (prl), a peptide hormone produced by the pituitary gland, was shown to play an important role in the modulation of the immune system of lower and higher vertebrates. to further investigate the effects of prl on the activation of professional phagocytes of bony fish, we stimulated head kidney leukocytes and purified macrophages from the gilthead seabream (sparus aurata l.) with various physiological concentrations of native salmon prl for 2 and 16 h and analyzed the respiratory burst ac ... | 2010 | 20826756 |
| adrenocorticotrophic hormone-stimulated cortisol release by the head kidney inter-renal tissue from sea bream (sparus aurata) fed with linseed oil and soyabean oil. | the mode of action of highly unsaturated fatty acids (hufa) in regulating gilthead sea bream (sparus aurata) head kidney (hk) cortisol production was studied through in vitro trials using a dynamic superfusion system. fish were previously fed with different diets containing several inclusion levels of linseed oil (lo) or soyabean oil (so) for 26 weeks. five diets were tested; anchovy oil was the only lipid source for the control diet (fish oil, fo) and two different substitution levels (70 and 1 ... | 2011 | 20836902 |
| scarcity of parasite assemblages in the adriatic-reared european sea bass (dicentrarchus labrax) and sea bream (sparus aurata). | the shaping forces of parasite community structure still is the main subject in the ecological parasitology whilst community predictability and repeatability showed that hardly a generally applicable role is ever going to be assessed. defining and describing parasite communities can be very useful from the epizootiological point, in order to help in the assessment of the medical and economical impact of certain parasitosis, moreover when hosts are economically valuable species. since parasite as ... | 2010 | 20837380 |
| cellular response in semi-intensively cultured sea bream gills to ergasilus sieboldi (copepoda) with emphasis on the distribution, histochemistry and fine structure of mucous cells. | light and ultrastructure studies were carried out on gill of sea bream, sparus aurata l., naturally infected with ergasilus sieboldi (copepoda) to assess pathology and host cell responses. thirty s. aurata were examined, and 23 (74%) were infected, the intensity of infection ranging from 3 to 50 parasites per host. the copepod encircled gill lamellae with its second antennae, occluded arteries, compressed the epithelium, provoked hyperplasia and haemorrhage, and often caused tissue disruption. a ... | 2010 | 20850928 |
| a comparative study on the composition and importance of free amino acids in semen of gilthead sea bream, sparus aurata, and perch, perca fluviatilis. | a comparative study was conducted on the free amino acid composition of gilthead sea bream, sparus aurata, and perch, perca fluviatilis. also the effect of 21 free amino acids on sperm motility was investigated. spermatozoa were incubated in species-specific motility-inhibiting saline solution containing the different amino acids for 48 h. thereafter, the motility was activated and investigated using computer-assisted cell motility analysis. twelve free amino acids, respectively, were detected i ... | 2010 | 20924668 |
| physiological stress and innate immune response in gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) exposed to combination of trimethoprim and sulfamethoxazole (tmp-smx). | the purpose of this study was to determine whether gilthead sea bream and sea bass treated with combination of trimethoprim and sulfamethoxazole (tmp-smx) differed in terms of physiological and innate immune biomarkers. fish were exposed to tmp-smx at 40 ppm concentration for 1 h as a prophylactic usage. plasma cortisol, glucose, electrolytes (ca, p, na, k, cl, and mg) as well as plasma lysozyme activity, c-reactive protein (crp), and ceruloplasmin (cp) were measured soon after treatment and fol ... | 2011 | 20924669 |
| metabolic and osmoregulatory changes and cell proliferation in gilthead sea bream (sparus aurata) exposed to cadmium. | the impact of cadmium on metabolism and osmoregulation was assessed in gilthead sea bream (sparus aurata). seawater acclimated fish were injected intraperitoneally with a sublethal dose of cadmium (1.25 mg cd/kg body wt). after 7 days, half of the injected fish were sampled. the remaining fish were transferred to hypersaline water and sampled 4 days later. gill and kidney na(+)/k(+)-atpase activities, plasma levels of cortisol, several metabolites and osmolytes, as well as osmolality were measur ... | 2011 | 20933284 |
| influence of moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (sparus aurata, l.). | abstract: | 2010 | 20939867 |
| quantitative trait loci for resistance to fish pasteurellosis in gilthead sea bream (sparus aurata). | fish pasteurellosis is a bacterial disease causing important losses in farmed fish, including gilthead sea bream, a teleost fish of great relevance in marine aquaculture. we report in this study a qtl analysis for resistance to fish pasteurellosis in this species. an experimental population of 500 offspring originating from eight sires and six dams in a single mass-spawning event was subjected to a disease challenge with photobacterium damselae subsp. piscicida (phdp), the causative agent of fis ... | 2010 | 20946317 |
| the nutritional background of the host alters the disease course in a fish-myxosporean system. | the aim of the present work was to determine if a practical plant protein-based diet containing vegetable oils (vo) as the major lipid source could alter the disease course when challenged with the myxosporean enteromyxum leei, a wide-spread parasite in the mediterranean basin causing heavy economic losses. gilthead sea bream (sparus aurata) fed for 9 months either a fish oil (fo) diet or a blend of vos at 66% of replacement (66vo diet) were challenged by exposure to parasite-contaminated water ... | 2010 | 20947256 |
| changes in complement responses in gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) under crowding stress, plus viral and bacterial challenges. | gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) were subjected to either experimental infection with photobacterium damselae subsp. piscicida or nodavirus after a period of 2 weeks of crowding in which fish were subjected to a 5-fold increase in density (10-50 kg/m(3)). samples were obtained before the crowding period (0 h or control) and at 24h and 72 h after crowding from both groups of infected fish. the complement haemolytic activity and the expression of the c3 ... | 2010 | 20951810 |
| a comparative bac map for the gilthead sea bream (sparus aurata l.). | this study presents the first comparative bac map of the gilthead sea bream (sparus aurata), a highly valuated marine aquaculture fish species in the mediterranean. high-throughput end sequencing of a bac library yielded 92,468 reads (60.6 mbp). comparative mapping was achieved by anchoring bac end sequences to the three-spined stickleback (gasterosteus aculeatus) genome. bacs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. a fraction of 5,249 bacs ... | 2011 | 21049003 |
| patterns of gastric evacuation, digesta characteristics and ph changes along the gastrointestinal tract of gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.). | a comparative study of gastric evacuation rates (gers) and digesta content, moisture and ph values along the gastrointestinal tract was performed between gilthead sea bream and european sea bass. in order to distinguish species-specific differences from diet-elicited effects, all parameters were determined under either a fishmeal diet or a carob seed germ meal diet that contained high levels of total and soluble non-starch polysaccharides. gers were significantly different between species and th ... | 2011 | 21130892 |
| changes in adipocyte cell size, gene expression of lipid metabolism markers, and lipolytic responses induced by dietary fish oil replacement in gilthead sea bream (sparus aurata l.). | the effects of fish oil (fo) substitution by 66% vegetable oils in a diet with already 75% vegetable protein (66vo) on adipose tissue lipid metabolism of gilthead sea bream were analysed after a 14-month feeding trial. in the last 3 months of the experiment, a fo diet was administrated to a 66vo group (group 66vo/fo) as a finishing diet. hormone-sensitive lipase (hsl) activity was measured in adipose tissue and adipocyte size, and hsl, lipoprotein lipase and liver x receptor gene expression in i ... | 2011 | 21130894 |
| divergence of duplicate pomc genes in gilthead sea bream sparus auratus. | proopiomelanocorticotrophin (pomc) in vertebrates is produced in the pituitary gland and undergoes post-translational processing to give rise to a range of biologically active peptides. teleosts possess 2-3 different pomc transcripts which have been proposed to have originated from a whole or partial genome duplication. in the present study 2 transcripts of gilthead sea bream pomc (sbpomc-α1 and α2) were cloned and characterised. sbpomc-α1 is expressed principally in the melanotroph cells of the ... | 2011 | 21147111 |
| novel horizontal transmission route for enteromyxum leei (myxozoa) by anal intubation of gilthead sea bream sparus aurata. | the aim of the present study was to determine whether enteromyxum leei, one of the most threatening parasitic diseases in mediterranean fish culture, could be transmitted by peranal intubation in gilthead sea bream sparus aurata l. fish were inoculated either orally or anally with intestinal scrapings of infected fish in 3 trials. oral transmission failed, but the parasite was efficiently and quickly transmitted peranally. prevalence of infection was 100% at 60 d post inoculation (p.i.) in trial ... | 2010 | 21166314 |
| recovery of opercular anomalies in gilthead sea bream, sparus aurata l.: morphological and morphometric analysis. | opercular anomalies are very frequent in reared gilthead sea bream and these can negatively influence the product value. field observations have suggested that opercular malformations can recover over time. in order to verify this hypothesis, 140-day-old gilthead sea bream with monolateral opercular anomalies were divided into three groups, according to the type and increasing seriousness of the opercular malformations, and another group was composed of fish with bilateral opercular anomalies. a ... | 2011 | 21166823 |
| annual variations of the plasmatic levels of glucose and amino acid and daily changes under different natural conditions of temperature and photoperiod in gilthead sea bream (sparus aurata, l.). | daily and annual changes in the plasmatic glucose and amino acid concentration have been determined in sparus aurata l. fish (average weight 330 g) were kept in cages under natural conditions of temperature and photoperiod and fed with a commercial diet. the months studied were chosen to establish whether there is any influence on the plasmatic glucose and amino acid concentration due to the change in temperature and photoperiod (equal photoperiod and different temperature, march and october; di ... | 2011 | 21174148 |
| cellular morphology and markers of cartilage and bone in the marine teleost sparus auratus. | modifications have been characterised in terms of cellular organisation and the extracellular matrix (ecm) during bone ontogeny in the sea bream (sparus auratus). during endochondral development, the agglomeration of matrix-secreting cells gives rise to chondrones; these chondrones frequently contain proliferating-cell-nuclear-antigen-positive cells, which subsequently become large collagen-ii-positive cells with the characteristics of chondrocytes. moreover, the matrix:cell ratio within the per ... | 2011 | 21234603 |
| quantitative trait loci involved in sex determination and body growth in the gilthead sea bream (sparus aurata l.) through targeted genome scan. | among vertebrates, teleost fish exhibit a considerably wide range of sex determination patterns that may be influenced by extrinsic parameters. however even for model fish species like the zebrafish danio rerio the precise mechanisms involved in primary sex determination have not been studied extensively. the zebrafish, a gonochoristic species, is lacking discernible sex chromosomes and the sex of juvenile fish is difficult to determine. sequential protandrous hermaphrodite species provide disti ... | 2011 | 21304996 |
| mast cell responses to ergasilus (copepoda), a gill ectoparasite of sea bream. | immunocytochemical, light microscopy and ultrastructural studies were conducted on gill of sea bream, sparus aurata l., naturally parasitized with the important parasitic copepod ergasilus sp. to assess pathology and cellular responses. thirty-seven s. aurata were examined from a fish farm; 26 (70%) were parasitized, with infection intensity ranging from 3 to 55 parasites per fish. hosts were divided into two groups, lightly infected fish (<15 parasites per fish) and heavily infected fish (>15 p ... | 2011 | 21316458 |
| hepatic steatosis in artificially fed marine teleosts. | in a comparison of livers in fish (sparus auratus and dicentrarchus labrax) feeding on natural sources of food with livers of artificially fed animals, a much higher c18:1/c22:6 ratio was observed in the latter. staining livers with oil red o showed extensive steatosis in artificially fed fish, but not in those naturally fed. juvenile artificially fed fish showed a more extensive steatosis and a higher mortality rate. in steatotic fish fed a natural diet for 2 months, the liver exhibited extensi ... | 1998 | 21361972 |
| molecular cloning and characterization of european seabass (dicentrarchus labrax) and gilthead seabream (sparus aurata) complement component c3. | we present the complete c3 cdna sequence of gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) and its molecular characterization with a descriptive analysis of their structural elements. we obtained one sequence for gilthead seabream (gsbc3) which encodes a predicted protein of 1656 amino acids, and two sequences for european seabass (esbc3_1 and esbc3_2) which encode two predicted proteins of 1654 and 1587 amino acids respectively. all sequences present the character ... | 2011 | 21421056 |
| microbial ecology of fish species ongrowing in greek sea farms and their watery environment. | the present study focuses on the bacteriological profile of both watery ecosystem and fishes from different north and central west greek fish-farms ongrowing euryhaline fish species. the natural microflora of the fish and the water of their ongrowing units in selected farms were studied for a period of 20 months. the analyzed samples were mainly sea bream (sparus aurata) 61.3% and sea bass (dicentrarchus labrax) 24%. in most of the watery ecosystems coming from the different sampling areas, tota ... | 2011 | 21421068 |
| insulin and igf-i effects on the proliferation of an osteoblast primary culture from sea bream (sparus aurata). | bone deformities in several fish species, like gilthead sea bream (sparus aurata), are currently a major problem in aquaculture. to gain knowledge of fish skeletal development, a primary cell culture has been established from sea bream vertebra. the initial fibroblastic phenotype of the cells changed to a polygonal shape during the culture, and the addition of an osteogenic medium promoted the deposition of minerals in the extracellular matrix. cell proliferation was analyzed using the mtt assay ... | 2011 | 21447336 |
| differential effects on proliferation of gh and igfs in sea bream (sparus aurata) cultured myocytes. | primary culture of gilthead sea bream skeletal muscle cells was used to examine the effects of growth hormone (gh) and insulin-like growth factors (igfs) in fish muscle proliferation and growth. proliferation was measured as the percentage of positive cells expressing the proliferating cell nuclear antigen (pcna) analyzed by immunocytochemistry. first, the effects of gh from two different origins (mammals and fish) were tested. gh from human (hgh) did not stimulate proliferation except at 3h at ... | 2011 | 21458458 |
| cellular responses in the skin of the gilthead sea bream sparus aurata l. and the sea bass dicentrarchus labrax (l.) exposed to high ammonia. | adult gilthead sea bream sparus aurata and sea bass dicentrarchus labrax were exposed for 24 and 48 h, respectively, to two concentrations of ammonia each (mean values of 3·34 and 13·10 mg l(-1) ta-n in s. aurata; 2·99 and 11·90 mg l(-1) ta-n in d. labrax). light microscopy and computerized morphometry were used to evaluate ammonia-induced alterations in skin structure during exposure and following recovery in normal water. in s. aurata, ammonia exposure induced a concentration-dependent increas ... | 2011 | 21463312 |
| feed restriction up-regulates uncoupling protein 3 (ucp3) gene expression in heart and red muscle tissues of gilthead sea bream (sparus aurata l.) new insights in substrate oxidation and energy expenditure. | the physiological regulation of the mitochondrial uncoupling protein 3 (ucp3) remains practically unexplored in fish and the aim of this study was to examine the effects of ration size on the regulation of ucp3 in heart, red skeletal muscle and white skeletal muscle of gilthead sea bream (sparus aurata l.). juvenile fish were fed at three different levels for 11 weeks: i) full ration until visual satiety (r(100) group), ii) 70% of satiation (r(70) group) and iii) 70% of satiation with two finish ... | 2011 | 21463702 |
| copper induces cu-atpase atp7a mrna in a fish cell line, saf1. | copper transporting atpase, atp7a, is an atp dependent copper pump present in all vertebrates, critical for the maintenance of intracellular and whole body copper homeostasis. effects of copper treatment on atp7a gene expression in fibroblast cells (saf1) of the sea bream (sparus aurata) were investigated by qrt-pcr and by a medium density microarray from a closely related species, striped sea bream (lithognathus mormyrus). to discriminate between the effects of cu and other metals, saf1 cells w ... | 2011 | 21473932 |
| the influence of certain aminoacids and vitamins on post-thaw fish sperm motility, viability and dna fragmentation. | during cryopreservation, dilution in the extender media reduces the seminal plasma constituents being cells more vulnerable to oxidative stress. vitamins (c and e) and the amino acids taurine and hypotaurine are powerful antioxidants naturally present in seminal plasma. whether their effect may improve sperm quality and reduce sperm dna damage after cryopreservation in fish sperm still remains unclear. thus, the aim of the present work was to analyse the effect of extender supplementation with s ... | 2011 | 21482049 |
| molecular and functional characterization of the gilthead seabream β-defensin demonstrate its chemotactic and antimicrobial activity. | antimicrobial peptides (amps) are important mediators of the innate immune response against bacteria and viruses. we have found a β-defensin (bd) gene searching the expressed sequence tags (ests) of the teleost fish gilthead seabream (sparus aurata). the clone contains an open reading frame of 201bp mrna that encodes a putative seabream β-defensin (sabd) propeptide of 66 amino acids containing the six conserved cysteines as the main signature of this amp. the phylogenetic tree shows that sabd, a ... | 2011 | 21497909 |
| first report of streptococcus iniae in red porgy (pagrus pagrus, l.). | this work describes the first isolation of streptococcus iniae in red porgy, pagrus pagrus (l.), and the first european isolation of this pathogen in gilthead seabream, sparus aurata (l.). in both farmed fish species, infection resulted in lethargy, anorexia, abnormal swimming, exophthalmia and sudden death, with mortality rates of over 25% in red porgy and 10% in gilthead seabream. beta-haemolytic gram-positive cocci, catalase negative and oxidase negative, were isolated in pure culture from in ... | 2010 | 21504081 |
| gilthead seabream (sparus aurata) carrying antibiotic resistant enterococci. a potential bioindicator of marine contamination? | antibiotic resistance in bacteria is a growing problem that is not only restricted to the clinical setting but also to other environments such as marine species that harbor antibiotic resistant bacteria and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to evaluate antibiotic resistance phenotypes in enterococci isolated from fecal samples of gilthead seabream and the associated mechanisms of resistance. a collection of 118 samples wer ... | 2011 | 21511306 |
| collagen fibrils in cultured and wild sea bream (sparus aurata) liver. an electron microscopy and image analysis study. | this study aims to measure liver collagen fibril diameter in cultured and wild sea breams (sparus aurata). cultured sea breams were fed three isonitrogenous diets. the organically produced feed contained sustainable certified fish meal (45%), fish oil (14%), and organic certified wheat; the laboratory feed contained fish meal (45%), fish oil (14%), wheat meal, and soya meal; and the commercial feed included fish meal (46%), fish oil (17%), soya meal, wheat meal, and corn gluten meal. the organic ... | 2011 | 21516288 |
| cadmium accumulation and antioxidant responses in sparus aurata exposed to waterborne cadmium. | cadmium (cd), a nonessential trace element, is rapidly accumulated by most living organisms and subsequently exerts its toxicity at different molecular levels. this study exposed gilthead sea bream (sparus aurata) to waterborne 0.1 mg/l cd for 11 days and investigated the cd accumulation pattern, lipid oxidation, and response of antioxidant defences. at the end of the experiment, mean cd concentrations in gills and liver, the organs most prone to metal accumulation, were 209.4 and 371.7 ng/g ww, ... | 2012 | 21553241 |
| functional divergences of gapdh isoforms during early development in two perciform fish species. | glyceraldehyde-3-phospate dehydrogenase (gapdh) is involved in basic cell catabolic processes and, as it is thought to be continuously expressed, belongs to the group of housekeeping genes. thus, it is frequently used as an internal control in quantitative gene expression studies. however, the evidence of different expression patterns in a broad range of organisms and tissues, as well as the occurrence of different isoforms, shows that gapdh has to be reevaluated as an internal control in qpcr s ... | 2011 | 21559871 |
| mast cells in the intestine and gills of the sea bream, sparus aurata, exposed to a polychlorinated biphenyl, pcb 126. | the presence of mast cells has been reported in all classes of vertebrates, including many teleost fish families. the mast cells of teleosts, both morphologically and functionally, show a close similarity to the mast cells of mammals. mast cells of teleosts, localized in the vicinity of blood vessels of the intestine, gills and skin, may play an important role in the mechanisms of inflammatory response, because they express a number of functional proteins, including piscidins, which are antimicr ... | 2012 | 21565388 |
| mx expression in gilthead sea bream (sparus aurata l.) in response to poly i:c, bacterial lps and chromosomal dna: preliminary study. | 2011 | 21575726 | |
| hepatocyte nuclear factor 4α transactivates the mitochondrial alanine aminotransferase gene in the kidney of sparus aurata. | alanine aminotransferase (alt) plays an important role in amino acid metabolism and gluconeogenesis. the preference of carnivorous fish for protein amino acids instead of carbohydrates as a source of energy lead us to study the transcriptional regulation of the mitochondrial alt (malt) gene and to characterize the enzyme kinetics and modulation of malt expression in the kidney of gilthead sea bream (sparus aurata) under different nutritional and hormonal conditions. 5'-deletion analysis of malt ... | 2012 | 21607544 |
| in silico mining and characterization of simple sequence repeats from gilthead sea bream (sparus aurata) expressed sequence tags (est-ssrs); pcr amplification, polymorphism evaluation and multiplexing and cross-species assays. | we screened for simple sequence repeats (ssrs) found in ests derived from an est-database development project ('marine genomics europe' network of excellence). different motifs of di-, tri-, tetra-, penta- and hexanucleotide ssrs were evaluated for variation in length and position in the expressed sequences, relative abundance and distribution in gilthead sea bream (sparus aurata). we found 899 ests that harbor 997 ssrs (4.94%). on average, one ssr was found per 2.95 kb of est sequence and the d ... | 2011 | 21620329 |
| aquaporin inhibition changes protein phosphorylation pattern following sperm motility activation in fish. | our previous studies demonstrated that osmolality is the key signal in sperm motility activation in sparus aurata spermatozoa. in particular, we have proposed that the hyper-osmotic shock triggers water efflux from spermatozoa via aquaporins. this water efflux determines the cell volume reduction and, in turn, the rise in the intracellular concentration of ions. this increase could lead to the activation of adenylyl cyclase and of the camp-signaling pathway, causing the phosphorylation of sperm ... | 2011 | 21620454 |
| molecular characterization and expression analysis of six peroxiredoxin paralogous genes in gilthead sea bream (sparus aurata): insights from fish exposed to dietary, pathogen and confinement stressors. | the aim of this work was to underline the physiological role of the antioxidant peroxiredoxin (prdx) family in gilthead sea bream (sparus aurata l.), a perciform fish extensively cultured in the mediterranean area. first, extensive blast searches were done on the gilthead sea bream cdna database of the aquamax european project (www.sigenae.org/iats), and six contigs were unequivocally identified as prdx1-6 after sequence completion by rt-pcr. the phylogenetic analysis evidenced three major clade ... | 2011 | 21640832 |
| gilthead seabream (sparus aurata) as carriers of shv-12 and tem-52 extended-spectrum beta-lactamases-containing escherichia coli isolates. | abstract in recent years, bacterial resistance to beta-lactam antibiotics has risen dramatically in escherichia coli isolated from animals that could pass through the food chain to humans. one hundred eighteen fecal samples of sparus aurata were tested for extended-spectrum beta-lactamase (esbl)-containing e. coli recovery. susceptibility to 16 antimicrobial agents was performed by disk diffusion. esbl-phenotypic detection was carried out by double-disk test, and the presence of genes encoding t ... | 2011 | 21657938 |
| biological effects of marine contaminated sediments on sparus aurata juveniles. | chemical analysis of the compounds present in sediment, although informative, often is not indicative of the downstream biological effects that these contaminants exert on resident aquatic organisms. more direct molecular methods are needed to determine if marine life is affected by exposure to sediments. in this study, we used an aquatic multi-species microarray and q-pcr to investigate the effects on gene expression in juvenile sea bream (sparus aurata) of two contaminated sediments defined as ... | 2011 | 21658360 |
| differential expression of pthrp and its receptor in pituitary gland and gills in estradiol-treated gilthead sea bream (sparus auratus, l.). | in the gilthead sea bream (sparus auratus) 17β-estradiol (e₂) plays an important role in the synthesis of vitellogenin. during vitellogenesis, vitellogenin as a nutritional precursor protein is loaded with calcium, which requires elevated plasma calcium levels. this is accomplished via e₂-dependent processes. reports have shown that hypercalcemic effects of e₂ are possibly mediated by another hypercalcemic factor, viz. parathyroid hormone related protein (pthrp). to further investigate the possi ... | 2011 | 21658390 |
| host-parasite relationship of ceratomyxa puntazzi n. sp. (myxozoa: myxosporea) and sharpsnout seabream diplodus puntazzo (walbaum, 1792) from the mediterranean with first data on ceratomyxid host specificity in sparids. | sparidae are economically important fishes to both, fisheries and aquaculture in the mediterranean. species diversification is an important strategy for the development of mediterranean aquaculture. one of the species recently introduced is the sharpsnout seabream diplodus puntazzo (walbaum, 1792). during a parasitological study of fish from the gulf of valencia and the mar menor (spain), myxozoan spores belonging to the genus ceratomyxa were found in the gall bladder of d. puntazzo. a morpholog ... | 2011 | 21680098 |
| influence of the diet on the microbial diversity of faecal and gastrointestinal contents in gilthead sea bream (sparus aurata) and intestinal contents in goldfish (carassius auratus). | fish intestinal microbiota changes with the diet and this effect is of particular interest considering the increasing substitution of fish meal by plant protein sources. the objective of this work was to study the effects of partial substitution of fish meal with lupin and rapeseed meals on gut microbiota of the gilthead sea bream (sparus aurata) and in goldfish (carassius auratus). faecal, gastrointestinal and intestinal contents were characterized using culture-based and molecular methods. vib ... | 2011 | 21692817 |
| species specificity in the magnitude and duration of the acute stress response in mediterranean marine fish in culture. | the aim of the present study was to examine the species-specific stress response for seven mediterranean fishes in culture. also, to evaluate the method of measuring free cortisol concentration in the rearing water as a non-invasive and reliable indicator of stress in marine species, of aquaculture importance. gilthead sea bream, sparus aurata (sparidae); common dentex, dentex dentex (sparidae); common pandora, pagellus erythrinus (sparidae); sharpsnout sea bream, diplodus puntazzo (sparidae); d ... | 2011 | 21712040 |
| gyrodactylus longipes n. sp. (monogenea: gyrodactylidae) from farmed gilthead seabream (sparus aurata l.) from the mediterranean. | gyrodactylus longipes n. sp. (monogenea, gyrodactylidae) is described from the gills of farmed juvenile gilthead seabream (sparus aurata l.) from two sites located in italy and bosnia-herzegovina and represents the second species of gyrodactylus to be described from s. aurata. gyrodactylus orecchiae paladini, cable, fioravanti, faria, di cave et shinn, 2009 was the first gyrodactylid to be described from s. aurata, from populations cultured in albania and croatia. in the current study, g. longip ... | 2011 | 21723959 |
| proteomics in the liver of gilthead sea bream (sparus aurata) to elucidate the cellular response induced by the intake of maslinic acid. | maslinic acid (ma) is a pentacyclic triterpene used as a feed additive to stimulate growth, protein-turnover rates, and hyperplasia in fish. to further our understanding of cellular mechanisms underlying the action of ma, we have used 2-de coupled with ms to identify proteins differentially expressed in the livers of juvenile gilthead sea bream (sparus aurata) grown under fish-farm conditions and fed with a 100 mg/kg ma-enriched diet (ma(100)). after the comparison of the protein profiles from m ... | 2011 | 21751345 |
| infiltration and activation of acidophilic granulocytes in skin lesions of gilthead seabream, sparus aurata, naturally infected with lymphocystis disease virus. | light, ultrastructural and immunocytochemical investigations were carried out on the skin of gilthead seabream, sparus aurata l., naturally infected with lymphocystis iridovirus, to assess pathology and host cellular responses. of 220,000 young seabream examined, 32,400 (14.7%) had clinical signs of lymphocystis and within 6months of disease appearance, 45% of clinically affected fish had died. a subsample of 20 s. aurata (80.0±12.5mm total length, mean±s.d.), including 10 with lymphocystis on t ... | 2011 | 21762724 |
| immune response of gilthead seabream (sparus aurata) following experimental infection with aeromonas hydrophila. | the gram-negative bacteria aeromonas hydrophila is a heterogeneous organism that causes the disease known as motile aeromonad septicaemia, which is responsible for serious economic loss in seabream culture due to bacterial infections. however, the immune mechanisms involved in this disease in fish are still poorly understood. for the purpose of this study, gilthead seabream (sparus aurata l.) specimens received a double intraperitoneal injection of bacterial inoculums: a primary infection with 1 ... | 2011 | 21767651 |
| accumulation of ivermectin in the brain of sea bream, sparus aurata after intraperitoneal administration. | ivermectin, which is widely used in veterinary and human, has been considered safe due to its inability to penetrate into the central nervous system of higher vertebrates. this paper presents data on the ability of the drug to cross the blood-brain barrier of the marine teleost sea bream, sparus aurata and accumulate in the brain. the concentration of the drug in the brain and the serum of the fish was assessed by the use of a direct competitive elisa commercial kit. our results showed a rapid u ... | 2004 | 21782707 |
| gilthead sea bream (sparus auratus) and european sea bass (dicentrarchus labrax) expressed sequence tags: characterization, tissue-specific expression and gene markers. | the gilthead sea bream, sparus auratus, and the european sea bass, dicentrarchus labrax, are two of the most important marine species cultivated in southern europe. this study aimed at increasing genomic resources for the two species and produced and annotated two sets of 30,000 expressed sequence tags (est) each from 14 normalized tissue-specific cdna libraries from sea bream and sea bass. clustering and assembly of the ests formed 5268 contigs and 12,928 singletons for sea bream and 4573 conti ... | 2010 | 21798212 |
| molecular profiling of the gilthead sea bream (sparus aurata l.) response to chronic exposure to the myxosporean parasite enteromyxum leei. | the aim of the present work was to investigate the transcriptome response of gilthead sea bream (sparus aurata) after challenge with the myxosporean enteromyxum leei, a wide-spread enteric parasite causing heavy economic losses in mediterranean sparid farms. this parasite causes severe desquamative enteritis which usually leads to death of the fish, and there are no preventative or curative treatments for this enteromyxosis. after 113 days of exposure to parasite-contaminated effluent, fish were ... | 2011 | 21803425 |
| analysis of the genetic diversity of the lymphocystis virus and its evolutionary relationship with its hosts. | lymphocystis disease virus (lcdv) is the causative agent of lymphocystis disease. in this study, the mcp gene of lcdv and the cyt b gene of the host fish were selected as molecular markers, and the phylogenetic relationships between lcdv and its host were analyzed. the 25 lcdv isolates examined in this study were attributed to seven lcdv genotypes: genotype i (lcdv-1), genotype ii (lcdv-cn, etc.), genotype iii (lcdv-rf), genotype iv (lcdv-rc and lcdv-sb), genotype v (lcdv-cb), genotype vi (lcdv- ... | 2011 | 21805162 |
| stable isotope analysis combined with metabolic indices discriminates between gilthead sea bream (sparus aurata) fingerlings produced in various hatcheries. | there are few traceability systems other than genetic markers capable of distinguishing between sea products of different origin and quality. here, we address the potential of stable isotopes combined with metabolic and growth parameters as a discriminatory tool for the selection of fish seeds with high growth capacity. for this purpose, sea bream fingerlings produced in three hatcheries (spanish mediterranean coast, mc; cantabrian coast, cc; and south-iberian atlantic coast, ac) were subjected ... | 2011 | 21838305 |
| Differential antiviral activity of Mx1, Mx2 and Mx3 proteins from gilthead seabream (Sparus aurata) against Infectious Pancreatic Necrosis Virus (IPNV). | Mx proteins are crucial effectors of the innate antiviral response mediated by the interferon type I signalling pathway. Recently, three Mx proteins, named SauMx1, SauMx2 and SauMx3, corresponding to three different genes, have been identified in the cultured marine species gilthead seabream (Sparus aurata). In this study, the three SauMx cDNAs were cloned into expression vectors and used for transfection of CHSE-214 cells. Monoclonal cell populations stably expressing each recombinant protein h ... | 2011 | 21872932 |
| effects of waterborne cu exposure in gilthead sea bream (sparus aurata): a proteomic approach. | aquatic organisms may suffer from exposure to high cu concentrations, since this metal is widely used in feed supplementation, in pesticide formulation and as antifouling. chronic exposure to cu, even at sub-lethal doses, may strongly affect fish physiology. to date, several biomarkers have been used to detect cu exposure in fish producing contrasting results. therefore, we used a proteomic approach to clarify how cu exposure may affect the serum proteome of gilthead sea bream (sparus aurata), s ... | 2011 | 21925607 |
| a microsatellite linkage map of striped bass (morone saxatilis) reveals conserved synteny with the three-spined stickleback (gasterosteus aculeatus). | the striped bass (morone saxatilis) and its relatives (genus morone) are of great importance to fisheries and aquaculture in north america. as part of a collaborative effort to employ molecular genetics technologies in striped bass breeding programs, we previously developed nearly 500 microsatellite markers. the objectives of this study were to construct a microsatellite linkage map of striped bass and to examine conserved synteny between striped bass and three-spined stickleback (gasterosteus a ... | 2012 | 21968826 |
| skin healing and scale regeneration in fed and unfed sea bream, sparus auratus. | fish scales are an important reservoir of calcium and phosphorus and together with the skin function as an integrated barrier against environmental changes and external aggressors. histological studies have revealed that the skin and scales regenerate rapidly in fish when they are lost or damaged. in the present manuscript the histological and molecular changes underlying skin and scale regeneration in fed and fasted sea bream (sparus auratus) were studied using a microarray 3 and 7 days after s ... | 2011 | 21981800 |
| the effects of extraction methods on the contents of fatty acids, especially epa and dha in marine lipids. | this study aims to evaluate the efficiency of trans-methylation methods in fish oil obtained using soxhlet extraction method and bligh and dyer method and also to observe the effects of extraction methods on the contents of polyunsaturated fatty acids [pufas, especially eicosapentaenoic acid (epa) and docosahexaenoic acid (dha)] in five marine species: sardine (sardinella aurita), anchovy (engraulis encrasicolus), sea bream (sparus aurata), brushtooth lizardfish (saurida undosquamis) and chub ma ... | 2012 | 22010951 |
| Post-larval development of the microcotylid monogenean Sparicotyle chrysophrii (Van Beneden and Hesse, 1863): comparison with species of Microcotylidae and Heteraxinidae. | The chronology of post-larval development in S. chrysophrii, a polyopisthocotylean monogenean parasite of the gilthead seabream (Sparus aurata L.), was experimentally studied. It is compared with other species within the Microcotylidae and the Heteraxinidae, including an analysis of the changes in attachment and the growth rate. Gilthead seabreams infected by larvae of S. chrysophrii were killed periodically in order to collect the different developmental stages. Parasite total body length, hapt ... | 2011 | 22015332 |
| heterozygosity-fitness correlations in the gilthead sea bream sparus aurata using microsatellite loci from unknown and gene-rich genomic locations. | heterozygosity-fitness correlations (hfc) were assessed for a sample of a gilthead sea bream sparus aurata population. two hundred and seventy-one fish were genotyped at 22 known and novel microsatellite loci, from which correlations between the multilocus heterozygosity index (i(mlh) ) and various fitness traits (fork length, mass and specific growth rates) were calculated. significant global hfcs were found in this sample (0·02 ≤r(2) ≤ 0·08). in addition, all the significant correlations found ... | 2011 | 22026596 |
| Discovery of novel biopreservation agents with inhibitory effects on growth of food-borne pathogens and their application to seafood products. | Selection of protective cultures is relevant in order to biopreserve and improve the functional safety of food products, mainly through inhibition of spoilage and/or pathogenic bacteria. Accordingly, the present study investigated potential applications of lactic acid bacteria (LAB) in the biopreservation of fish and shellfish products. For this purpose, a collection of 84 LAB strains isolated from sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) was identified and characterized for ... | 2011 | 22041547 |
| trace elements and metals in farmed sea bass and gilthead bream from tenerife island, spain. | the aim of this study was to determine the levels of metals (ca, k, na, mg) and trace metals (ni, fe, cu, mn, zn, pb, cd) in two fish species (gilthead bream [sparus aurata] and sea bass [dicentrarchus labrax]) collected from fish farms located along the coast of tenerife island. ca, k, na, mg, fe, cu, zn, and mn were measured by flame atomic absorption spectrometry, whereas pb, cd, and ni were determined using graphite furnace atomic absorption spectrometry. mean fe, cu, mn, and zn contents wer ... | 2011 | 22054198 |
| gut ph as a limiting factor for digestive proteolysis in cultured juveniles of the gilthead sea bream (sparus aurata). | after the development of the gastric function in juvenile fish, dietary proteins enter a two-phase digestive process comprising an acidic gastric phase followed by an alkaline intestinal phase. however, the main gastric protease, pepsin, is strictly dependent on the existence of a low-enough environmental ph. in 20-g gilthead sea bream, sparus aurata, the mean minimal gastric ph is close to 4.5, while the mean ph in the duodenal portion of the intestine was nearly fixed at 6.5. the mean maximal ... | 2012 | 22086356 |
| fish polar lipids retard atherosclerosis in rabbits by down-regulating paf biosynthesis and up-regulating paf catabolism. | platelet activating factor (paf) has been proposed as a key factor and initial trigger in atherosclerosis. recently, a modulation of paf metabolism by bioactive food constituents has been suggested. in this study we investigated the effect of fish polar lipid consumption on paf metabolism. | 2011 | 22087726 |
| exploring neutral and adaptive processes in expanding populations of gilthead sea bream, sparus aurata l., in the north-east atlantic. | recent studies in empirical population genetics have highlighted the importance of taking into account both neutral and adaptive genetic variation in characterizing microevolutionary dynamics. here, we explore the genetic population structure and the footprints of selection in four populations of the warm-temperate coastal fish, the gilthead sea bream (sparus aurata), whose recent northward expansion has been linked to climate change. samples were collected at four atlantic locations, including ... | 2012 | 22126850 |
| aquaporin-4 immunoreactivity in müller and amacrine cells of marine teleost fish retina. | aquaporins (aqps) are membrane proteins that facilitate water transport across biological membranes and are essential for the proper function of neural tissue. although aqps have been extensively studied in mammalian retina, their presence in lower vertebrate retina is less frequently characterized. aqp4 expressed in mammalian and chick müller cells plays a major part in maintaining retinal homeostasis. in this study, we examined the immunoreactivity of aqp4 in the adult retina of gilthead sea b ... | 2012 | 22138430 |
| the effect of dietary methionine and white tea on oxidative status of gilthead sea bream (sparus aurata). | free radicals are continuously generated during an organism's lifetime. in order to understand the involvement in the oxidative status of fish, methionine and white tea were assayed as antioxidant supplements in diets for gilthead sea bream (sparus aurata). for the purpose of this study, four isonitrogenous and isolipidic diets were formulated to contain 45 % of protein and 18 % lipid and 0·3 % methionine (met diet), 2·9 % white tea dry leaves (tea diet) and 2·9 % of white tea dry leaves + 0·3 % ... | 2012 | 22153060 |
| transcriptomic characterization of the larval stage in gilthead seabream (sparus aurata) by 454 pyrosequencing. | gilthead seabream (sparus aurata) is a teleost belonging to the family sparidae with a high economical relevance in the mediterranean countries. although genomic tools have been developed in this species in order to investigate its physiology at the molecular level and consequently its culture, genomic information on post-embryonic development is still scarce. in this study, we have investigated the transcriptome of a marine teleost during the larval stage (from hatching to 60 days after hatchin ... | 2012 | 22160372 |
| comparison of two techniques for the morphometry study on gilthead seabream (sparus aurata) spermatozoa and evaluation of changes induced by cryopreservation. | the development of powerful software has made possible spermatozoa morphology studies. however, some problems have emerged in relation to protocol standardization to compare results from different laboratories. this study was carried out to compare two techniques commonly used (staining vs phase contrast technique) for the morphometry study of gilthead sea bream spermatozoa using an integrated sperm analysis system (isas). spermatozoa morphometry values were significantly affected by the techniq ... | 2012 | 22225681 |
| the effect of hypoxia on intermediary metabolism and oxidative status in gilthead sea bream (sparus aurata) fed on diets supplemented with methionine and white tea. | the present study evaluates the influence of previous nutritional status, fish fed on diets supplemented with tea and methionine, on acute hypoxia tolerance and subsequent recovery of sparus aurata juveniles. four isonitrogenous (45% of protein) and isolipidic (18% lipid) diets were formulated to contain 0.3% methionine, 2.9% white tea dry leaves or 2.9% of white tea dry leaves+0.3% methionine. an unsupplemented diet was used as control. hepatic key enzymes of intermediary metabolism and antioxi ... | 2012 | 22227440 |
| comparison among different gilthead sea bream (sparus aurata) farming systems: activity of intestinal and hepatic enzymes and 13c-nmr analysis of lipids. | in order to evaluate differences in general health and nutritional values of gilthead sea bream (sparus aurata), the effects of semi-intensive, land-based tanks and sea-cages intensive rearing systems were investigated, and results compared with captured wild fish. the physiological state was determined by measuring the activity of three different intestinal digestive enzymes: alkaline phosphatase (alp), leucine aminopeptidase (lap) and maltase; and the activity of the hepatic alp. also, the hep ... | 2009 | 22253985 |
| metabolomics as a powerful tool for molecular quality assessment of the fish sparus aurata. | the molecular profiles of perchloric acid solutions extracted from the flesh of sparus aurata fish specimens, produced according to different aquaculture systems, have been investigated. the (1)h-nmr spectra of aqueous extracts are indicative of differences in the metabolite content of fish reared under different conditions that are already distinguishable at their capture, and substantially maintain the same differences in their molecular profiles after sixteen days of storage under ice. the fi ... | 2011 | 22254093 |
| effects of dietary inulin and heat-inactivated bacillus subtilis on gilthead seabream (sparus aurata l.) innate immune parameters. | in the present study, a feeding trial was conducted to evaluate the effect of inulin and heat-inactivated bacillus subtilis, single or combined, on several innate immune activities of gilthead seabream (sparus aurata). forty-eight specimens were randomly assigned to four dietary treatments: 0 (control), inulin (10 g/kg, prebiotic group), b. subtilis (10(7) cfu/g, probiotic group), or b. subtilis + inulin (10(7) cfu/g + 10 g/kg, synbiotic group). after two and four weeks, six fish of each group w ... | 2012 | 22348912 |
| structural changes in marinated fillets of gilthead sea bream (sparus aurata l.) during vacuum storage at refrigerated temperature. | fillets of sparus aurata were marinated in a mixture of salt (5.5%) and citric acid (48.9 g/kg) for 1 h, then vacuum packed and refrigerated for 1, 5, 16, and 21 d. structural and ultrastructural parameters were evaluated in 6 specimens per time period. at day 1, the preservative solution appeared among the muscle fibers, thus increasing the interfibrillar spaces; cytoplasmic organelles were swollen and the sarcolemma-endomysium appeared very altered and electron-dense. at 5 to 10 d, the marinad ... | 2011 | 22417345 |
| multiresidue determination of veterinary drugs in aquaculture fish samples by ultra high performance liquid chromatography coupled to tandem mass spectrometry. | a simple, selective and fast multiresidue method was developed for the determination of 32 veterinary drug residues belonging to several families, in gilthead sea bream (sparus aurata) by ultra high performance liquid chromatography coupled to tandem mass spectrometry (uhplc-ms/ms). the extraction was based on modified quechers (quick, easy, cheap, effective, rugged and safe) procedure, using as extraction solution a mixture of acetonitrile and methanol (75:25, v/v), and it reduces sample handli ... | 2012 | 22465276 |
| quantitative trait loci for body growth and sex determination in the hermaphrodite teleost fish sparus aurata l. | gilthead sea bream (sparus aurata l.) is an important marine fish in mediterranean aquaculture. sex determination by age and/or body weight is a critical life-history trait, the genetic basis for which is largely unknown in this sequential hermaphrodite species. herein, we performed a partial genome scan to map quantitative trait loci (qtl) affecting body weight and sex using 74 informative microsatellite markers from 10 paternal half-sib families to construct nine linkage groups (lg). in total, ... | 2012 | 22497460 |