Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a multimicroelectrode system composed of independent glass micropipettes with an eccentric tip structure for simultaneous intracellular recording. | a glass multimicroelectrode system for simultaneous intracellular recordings is described in which four pieces of micropipettes with rectangular cross section are arranged in a square compartment. each tip position is shifted from the central axis of the electrode stem toward the crest line of its corner. such micropipettes are mechanically bundled to one another so that the horizontal spacing among tips can be made shorter than the electrode diameter and components are independently penetrable. ... | 1992 | 1601448 |
| [the therapy of swimbladder inflammation (renicola sphaerosporosis) of carp]. | in pond experiments the efficiency of different chemotherapy variants against swimbladder inflammation (sbi) was proved. only by feeding of fumagillin, specifically affecting myxosporidians, the infection of kidney with sphaerospora renicola as well as the force of sbi could be reduced. the chemotherapy with metronidazole remained inefficacious, whereas the permanent feeding with methylene blue prevented only the infection with bothriocephalus acheilognathi and probably lowered insignificantly t ... | 1992 | 1610018 |
| purification of the sex steroid-binding protein from common carp (cyprinus carpio) plasma. | 1. sex steroid-binding protein was purified from common carp plasma. 2. testosterone- and estradiol-binding activity existed at the same fraction eluted from gel sepharose cl-2b, deae-sephacel, hydroxylapatite and hplc. 3. the molecular weight of the sex steroid-binding protein was 194,000. 4. at 50% displacement the order in which the steroids displaced [3h]testosterone bound to the binding protein was as follows: androstenedione greater than estradiol-17 beta greater than 11-deoxy-17-hydroxyco ... | 1992 | 1611876 |
| a flow procedure to determine oxygen binding isotherms for low affinity and easily oxidized hemoglobins. | 1. the determination of binding isotherms for low affinity hemoglobins is particularly difficult because of rapid autoxidation. 2. in carp hb (ph 6 + ihp, 25 degrees c), one-quarter of the hemes are oxidized within 3 min, preventing the accurate determination of even p50 or x. 3. we circumvent this problem by rapidly flowing hbo2, initially at ph 9 (x = 1.4 microm), against a low ph buffer to bring the system rapidly to equilibrium in the low affinity form. diode-array spectrophotometry allows a ... | 1992 | 1611887 |
| reptilian class i major histocompatibility complex genes reveal conserved elements in class i structure. | the polymerase chain reaction was used to isolate clones with class i major histocompatibility complex sequences from fish (carp), amphibian (axolotl), and two species of reptile (lizard and snake). the lizard and snake clones were used to isolate class i cdna clones. all the sequences showed the expected evolutionary relatedness. the carp and axolotl clones and one lizard cdna clone lacked the first cysteine in the alpha 3 domain which in other class i heavy chains forms an intradomain disulfid ... | 1992 | 1612650 |
| the laboratory maintenance of sanguinicola inermis plehn, 1905 (digenea: sanguinicolidae). | methodology is described for the laboratory maintenance of the life-cycle of sanguinicola inermis, using common carp (cyprinus carpio) as the definitive host and lymnaea peregra as the intermediate host. lymnaea auricularia was also infected but is not considered to be a suitable laboratory host. tench (tinca tinca) were susceptible to infection at high cercarial doses but subsequent infection rates were low. goldfish (carassius auratus) and lymnaea stagnalis were refractory to laboratory infect ... | 1992 | 1614729 |
| risk factors for kidney cancer in new south wales, australia. ii. urologic disease, hypertension, obesity, and hormonal factors. | in a population-based case-control study of kidney cancer in new south wales, australia, data from structured interviews with 489 cases of renal cell cancer (rcc) and 147 cases of renal pelvic cancer (carp) diagnosed in 1989 and 1990, and 523 controls from the electoral rolls, confirmed the link between obesity and rcc. in addition, regular consumption of 'diet' pills independently increased the risk for this cancer. a diagnosis of hypertension at least two years before interview raised the risk ... | 1992 | 1617119 |
| inhibition of ca2+ uptake in freshwater carp, cyprinus carpio, during short-term exposure to aluminum. | in carp exposed to ph 5.2 in fresh water, the ca2+ influx from the water is reduced by 31% when compared to fish in water of neutral ph. at ph 5.2, the ca2+ influx but not na+ uptake is decreased by aluminum (al). al reduces ca2+ influx dose-dependently: a maximum 55% reduction was observed after 1-2 h exposure to 200 micrograms.1(-1) (7.4 microm) al. branchial ca2+ efflux is less sensitive to al and affected only by exposure for more than 1 h to high al concentrations. na+ influx is not affecte ... | 1992 | 1640197 |
| [histochemical investigations on the localization of acetylcholinesterase in the kidney of selected vertebrates]. | the light and electron microscopical localization of ache activity in the kidney of selected vertebrates was studied using the method of karnovsky and roots (1964) for light microscopy and the modification of the koelle and friedenwald's technique according to tsuji (1974, 1984) for electron microscopy. ache activity could be demonstrated light microscopically mainly within the glomeruli of some mammals (golden hamster, mouse, rat) and non-mammalian vertebrates (carp, frog). no activity was foun ... | 1992 | 1642103 |
| effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp cyprinus carpio in vitro. | isolated ovarian tissues from the common carp, cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, delta 5-3 beta-hsd and 17 beta-hsd activity. stage ii ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting onl ... | 1992 | 1642793 |
| chromaticity of synaptic inputs to h1 horizontal cells in carp retina: analysis by voltage-clamp and spectral adaptation. | cone photoreceptor inputs to h1 horizontal cells (h1 hcs) in carp retina were studied by measuring light-modulated currents (il) to monochromatic stimuli (460, 533, 688 nm) under a voltage-clamp condition. by using double-barrelled micro-electrodes h1 hcs were voltage-clamped whilst perfusing with dopamine to uncouple the cells. the il of the h1 hcs driven by each cone input was segregated by selective chromatic adaptation, and differences in the kinetics of the il of the h1 hcs were revealed. t ... | 1992 | 1644113 |
| [beta-adrenergic catecholamines activate the na+, k+ pump of carp erythrocytes independently of the stimulation of na+/h+ exchange]. | 1991 | 1648470 | |
| evidence for glycosylphosphatidylinositol anchoring of intralumenal alkaline phosphatase of the calf intestine. | 1. considerable amounts of intestinal alkaline phosphatase (ap) were found intralumenally in all animal species investigated, i.e. calf, pig, goat, rat, mouse, guinea pig, hen and carp. the ratios between the total activity of ap found intralumenally and the total intestinal activity vary considerably. calves and pigs show the highest, i.e. 0.77 and 0.44, respectively, while rodents have much lower ratios. only 20-34% of the intralumenal alkaline phosphatase (iap) of the calf and pig is soluble ... | 1991 | 1649047 |
| inhibition of mg2+ and na(+)-k+ atpases in selected tissues of fish, cyprinus carpio under fenvalerate toxicity. | the changes in the magnesium adenosine triphosphatase (mg2+ atpase) and sodium-potassium adenosine triphosphatase (na(+)-k+ atpase) in gill, brain, liver and muscle tissues of freshwater fish, cyprinus carpio at 6, 12, 24 and 48 hr exposure periods were studied after subjecting to sublethal concentration (10 micrograms/lit) of fenvalerate. mg2+ atpase and na(+)-k+ atpase activities were inhibited in all the tissues of fenvalerate exposed fish. the per cent inhibition increased with increase in t ... | 1991 | 1651727 |
| reversal potentials of color opponent responses in horizontal cells of the carp retina. | reversal potentials of color opponent responses were measured in h2 and h3 horizontal cells of the carp using ca2+ action potential method. all responses reversed around -5 mv, suggesting that the ionic mechanisms of light responses are common among all types of horizontal cells. glutamate-induced depolarizations in h2 and h3 cells had the same reversal potentials as those of light responses, consistent with the idea that these cells receive glutamate-mediated inputs from the cones. simultaneous ... | 1991 | 1654005 |
| an analysis of inputs to on-off amacrine cells in the carp retina with kynurenic acid. | amacrine cell inputs were studied in the carp retina. responses to light of bipolar and amacrine cells in off-pathways were suppressed by 0.5 mm kynurenic acid (kyn), while those in on-pathways were not. in on-off amacrine cells, the sustained potential level during illumination shifted in the depolarizing direction. the depolarizing response elicited in off-center bipolar cells by transretinal current was also suppressed, but those elicited in amacrine cells were not. the results indicated that ... | 1991 | 1655105 |
| corticostatic peptides. | in the last four years corticostatic (anti-acth) peptides have been isolated from human, rabbit, guinea pig and rat tissues. these peptides do not act via the camp cell signalling system but rather via the inhibition of the binding of acth to its receptor most probably through direct competition with the 14-18 sequence of acth for receptor binding. acth has specific high affinity receptors on adrenal cells but rabbit corticostatin i (csi) has high capacity, low affinity receptors which are compe ... | 1991 | 1659879 |
| [the spherical theory of color perception: its verification by the methods of psychophysics and neurophysiology]. | in the paper data of experimental verification of "spherical theory of perception" (e. n. sokolov) are summed on the material of colour perception study. the results of psychophysical experiments with subjects having different forms of colour perception (normal trichromats, colour anomalies) and neurophysiological experiments on animals (carp) are considered within single psychophysiological system--spherical model of colour discrimination. neuron-like elements of the model reflect the activity ... | 1991 | 1660642 |
| histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: atlantic croaker (micropogonias undulatus), spotted seatrout (cynoscion nebulosus), and red drum (sciaenops ocellatus). | the adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. in general greater specificity for the different cell types was observed with antisera to the piscine pituitary hormones than with antisera raised against the human hormones. the distribution of the cell types did not differ significantly among the three species, a ... | 1991 | 1667000 |
| regenerative sodium and calcium currents in goldfish retinal ganglion cell somata. | whole-cell patch-clamp recordings were made from isolated goldfish retinal ganglion cell somata. sodium- and calcium-dependent action potentials--blocked with tetrodotoxin (ttx) and co2+ ions, respectively--were evoked under current-clamp. depolarization of these somata under voltage clamp activated distinguishable na+ and ca2+ conductances: the former was permeable to na+, blocked by ttx, impermeable to n-methyl-d-glucamine and tris(hydroxymethyl)aminomethane, and began to activate around -45 m ... | 1991 | 1668863 |
| presence of prolactin receptors in eel liver and carp kidney and growth hormone receptor in eel liver. | 1. 125i-labelled ovine prolactin and bovine growth hormone were used to test for the presence of prolactin and growth hormone receptors in membrane prepared from tissues of the white eel anguilla japonica, the carp ctenopharynogodon idellus and the ricefield eel monopterus albus. 2. high levels of specific 125i-labelled ovine prolactin binding were found in white eel liver membranes and carp kidney membranes. 3. high levels of specific 125i-labelled bovine growth hormone binding were detected in ... | 1991 | 1678335 |
| [multiple molecular forms of glutamine synthetase in carp muscles]. | cytoplasmic and mitochondrial molecular forms of glutamine synthetase (ce 6.3.1.2) have been isolated from the carp muscle with purification degree of 100 and 165 times and output 9.0%. it is established that the temperature optimum of the cytoplasmic form activity is 30 degrees c and that of mitochondrial one--20 degrees c; the ph optimum for the both molecular forms is 6.0 and 8.2. the optimal ratio [me2+] : [atp] for the isolated form is 2:1; km (seeming) of the cytoplasmic form in the presen ... | 2015 | 1679261 |
| changes in early acoustic-evoked potentials by mildly arousing priming stimuli in carp (cyprinus carpio). | 1. averaged acoustic-evoked potential (aeps) in the medulla and midbrain were recorded, as were changes in heart rate, indicating arousal, to a previous non-acoustic priming stimulus. 2. useful aep measures were amplitude of the early biphasic wave (less than 10 msec) in medulla and amplitude and duration of this wave in midbrain. 3. there was a negative regression of heart rate and medullary aep amplitude especially evident for a 2 sec light stimulus. decreased aep amplitude in both regions was ... | 1991 | 1679693 |
| the effect of thyroid hormone and glucocorticoids on carp growth hormone-releasing factor (grf)-induced growth hormone (gh) release in rainbow trout (oncorhynchus mykiss). | 1. the effect of thyroid hormone and glucocorticoids on carp growth hormone-releasing factor (grf)-induced growth hormone (gh) secretion was studied on rainbow trout using a dispersed pituitary cell culture system. 2. a combined administration of lower doses (0.01 microm) of 3,5,3'-triiodo-l-thyronine (t3) and dexamethasone (dex) significantly increased spontaneous as well as carp grf-induced gh release. 3. lower doses of dex alone had no effect, and t3 had a marginal effect on gh release. highe ... | 2006 | 1679699 |
| the effect of large doses of vitamin c and magnesium on stress responses in common carp, cyprinus carpio. | 1. plasma magnesium, cortisol, lactate and ascorbic acid were examined in common carp subjected to various dietary treatments and following handling stress. 2. under conditions of satisfied dietary magnesium and ascorbate requirements, plasma cortisol concentration after stress increased less pronouncedly than in fish fed large doses of ascorbate and/or magnesium. 3. plasma lactate increased significantly in all groups after stress, although the increase seemed to be more severe (detrimental) in ... | 1991 | 1679707 |
| relationship of respiration to body weight in the carp cyprinus carpio under resting and normoxic condition. | relationships of oxygen consumption (vo2, ml/min/fish), ventilation volume (vg, ml/min/fish), stroke volume (vs, ml/stroke/fish), respiration frequency (rf, stroke/min) and oxygen utilization (u, %) to body weight (w, g) in the carp under resting and normoxic condition was vo2 = 0.004258 w 0.780710, vg = 1.035226 w 0.771371, vs = 0.045311 w 0.833989, rf = -0.002949 w + 17.847743, u = 0.0015423 w + 75.666757, respectively. | 1991 | 1682083 |
| changes in eeg power, acoustic evoked potentials and heart rate after mildly arousing non-acoustic priming stimuli in carp (cyprinus carpio). | 1. changes in eeg power spectrum of carp to a priming non-acoustic stimulus followed by acoustic clicks were compared to those due to acoustic clicks delivered alone. recordings were made from the telencephalon, midbrain and medulla. acoustic evoked potentials (aeps) to the clicks were also recorded. 2. eeg power changes to non-acoustic stimuli occurred over the whole 1-40 hz frequency range and were regionally specific and consistent. 3. the changes in the eeg midfrequency 12-24 hz power spectr ... | 1991 | 1682104 |
| sustained potential shifts and changes in acoustic evoked potentials after presentation of a non-acoustic priming stimulus to carp (cyprinus carpio). | 1. recordings were made from the region of the midbrain tectum and torus semicircularis of sustained potential shifts (sps) to a non-acoustic priming stimulus and the change in subsequent acoustic evoked potentials (aeps) to a train of six clicks after a long rest. 2. in the absence of priming stimuli (a jet of saline or water to the flank) the aep to the first click in a train had the highest amplitude; with these stimuli it became the most attenuated. 3. the sps to both non-acoustic stimuli wa ... | 1991 | 1682105 |
| interaction of carp growth hormone-releasing factor and somatostatin on in vitro release of growth hormone in rainbow trout (oncorhynchus mykiss). | possible antagonism between somatostatin (ss) and carp growth hormone-releasing factor (grf) on growth hormone (gh) secretion was examined by radioimmunoassay in a dispersed rainbow trout pituitary cell culture system. ss (3 nm) significantly antagonized carp grf(1-29; 1 nm, 10 nm)-induced gh secretion. the slope of the dose-response curve for carp grf(1-29) with ss was statistically different from that of carp grf(1-29) alone (p less than 0.05) suggesting a noncompetitive antagonism of ss to ca ... | 1991 | 1684643 |
| d-glucose permeability in river lamprey (lampetra fluviatilis) and carp (cyprinus carpio) erythrocytes. | 1. the transport of 3-o-methyl-d-glucose (3-omg) in lamprey and carp erythrocytes was studied. 2. in lamprey erythrocytes the half-time for the equilibration of 3-omg was fast, approx. 8 min. in contrast, the erythrocytes of carp were almost impermeable to 3-omg, with a half-time for equilibration of 14.2 hr. 3. 3-omg was taken up by lamprey erythrocytes both by facilitated diffusion and simple diffusion. the presence of carrier-mediated transport was indicated by saturation kinetics and by inhi ... | 1991 | 1685971 |
| mode of action of the molluscicide bromoacetamide. | 1. uptake of bromoacetamide was found to be upgraded with exposure time (6, 12, 24 hr) and drug concentration (0.1, 0.5, 1.0 ppm). 2. bromoacetamide was also absorbed by carp and mice, but drug concentration was markedly lower than in snails. drug clearance from carp as well as from mice is more rapid than that of snails. 3. the ultrastructure of hepatopancreas cells are markedly influenced by bromoacetamide, including swelling of mitochondria and splitting of cristae. this finding is correlativ ... | 1991 | 1687531 |
| physiological response of the fish, cyprinus carpio, to formalin exposure--i. effects of formalin on urine flow, heart rate, respiration. | 1. carp were exposed to 280 ppm formalin. haematocrit and plasma glucose and lactic acid increased. in moribund fish, blood ph was remarkably lower and plasma protein increased. 2. when exposed to formalin, heart rate (hr) and respiration increased briefly, and then decreased. 3. some fish increased urine flow (uf) immediately and maintained higher values for a while, followed by gradual decrease, and others decreased uf consistently. uf stopped at 30 min or longer prior to fish death. urine osm ... | 1991 | 1687535 |
| a variety of mytilus inhibitory peptides in the abrm of mytilus edulis: isolation and characterization. | 1. five species of mytilus inhibitory peptides, mip1-5, were isolated from acetone extracts of the anterior byssus retractor muscle (abrm) of mytilus edulis. mip1 and mip2 were shown to be s2-mip and a2-mip, respectively, first isolated from the pedal ganglia of the animal. 2. all the five peptides had a common c-terminal structure of -pro-xaa-phe-val-nh2, which was shown to be important for their biological activity. 3. the five mips showed similar inhibitory effects on contractions of the abrm ... | 1991 | 1687551 |
| ca2(+)-binding site of carp parvalbumin recognized by monoclonal antibody. | monoclonal antibody 235 which was used for immunohistochemical staining of parvalbumin in tissue sections partially protects lys-54 of carp muscle parvalbumin from reaction with acetic anhydride in the parvalbumin-antibody complex. lys-54 is located in the cd-loop of parvalbumin and is flanked by the ca2(+)-ligands asp-53 and ser-55 of the ca2(+)-site i. another monoclonal antibody against carp parvalbumin, mca 239, partially protects lysine residues 27, 32, 87 and 107, indicating that this anti ... | 1990 | 1690078 |
| a monoclonal antibody specific to müller cells and selective synaptic sites in the retina. | we have produced a monoclonal antibody which stains the müller cells in the region of the photoreceptors, nerve terminals surrounding the horizontal cells, and nerve terminals in the inner plexiform layer in carp, goldfish, and white perch retinas. electron microscopy showed that the staining in the outer and inner retina was confined to müller cells and presynaptic terminals, respectively. in the teleost brain, the antibody stained only the optic tectum and efferent fibers from the stratum albu ... | 1990 | 1692309 |
| turnover of serotonin in brain of an anoxia-tolerant vertebrate, the crucian carp. | serotonin (5-ht) is a neurotransmitter in the vertebrate brain. estimates of 5-ht turnover times range between 1 h (mammals) and 10 h (goldfish). synthesis and catabolism of 5-ht are dependent on molecular oxygen, hence, 5-ht turnover comes to a halt during anoxia. nevertheless, a few vertebrates, such as the crucian carp (carassius carassius l.), tolerate anoxia for several weeks. to assess the ability of the crucian carp brain to tolerate a stop in 5-ht turnover during anoxia, it is essential ... | 1990 | 1694412 |
| substrate specificity for myelin basic protein-specific protein methylase i. | the substrate specificity of bovine brain myelin basic protein (mbp)-specific protein methylase i (s-adenosyl-l-methionine:protein-l-arginine n-methyltransferase, ec 2.1.1.23), which methylates arginine residues of protein, has been studied using various mbps, several synthetic peptides and heterogeneous nuclear ribonucleoprotein complex protein (hnrnp). (1) among mbps from different species of brain, the carp mbp was found to be the best substrate for mbp-specific protein methylase i. this high ... | 1990 | 1694691 |
| mechanisms of temperature acclimation in the carp: a molecular biology approach. | most fish are unable to regulate their body temperature. therefore their muscle power output would be expected to decline markedly with decreased environmental temperature. however, some species including carp show a remarkable ability to acclimate. the nature and significance of changes in enzymatic myosin atpase activity and the recruitment of different muscle fiber types in relation to acclimation and environmental temperature in carp are reviewed. these changes presumably involve qualitative ... | 1990 | 1696790 |
| growth hormone-dependent potentiation of gonadotropin-stimulated steroid production by ovarian follicles of the goldfish. | the possible involvement of growth hormone (gh) in the regulation of ovarian function in the goldfish was investigated by determining the effects of common carp gh on steroid production by vitellogenic and preovulatory ovarian follicles incubated in vitro. carp gh acts in a dose-dependent manner to potentiate the actions of common carp gonadotropin (gth) on the production of 17 beta-estradiol and testosterone by vitellogenic ovarian follicles and the actions of human chorionic gonadotropin (hcg) ... | 1990 | 1697273 |
| development and validation of a competitive enzyme immunoassay for chum salmon prolactin: a comparison to radioimmunoassay. | an enzyme immunoassay (eia), based on a competitive assay system, for the measurement of prolactin (prl) in the pituitary of salmonid fishes and of hormone released in medium from incubated pituitary was developed using a rabbit antiserum to chum salmon prl (prl, a combination of prl i and prl ii). chum salmon prl was coupled to horseradish peroxidase (hrp). the incubation procedure for the antigen-antibody reaction was analogous to that in the radioimmunoassay (ria) for prl. the antibody-bound ... | 1990 | 1705233 |
| involvement of voltage-dependent calcium channels (vdcc) in the action of gnrh on gth release in common carp (cyprinus carpio l): comparison with k+ action. | the involvement of different types of voltage-dependent calcium channels (vdcc) in the stimulatory action of gnrh (in comparison with k+) on maturational gonadotropin (gth) release was investigated using superfused carp pituitary cells. the action of these 2 stimulants was not modified either by d600 or nifedipine (drugs blocking l-type of vdcc). cadmium (cd2+), which blocks all types of vdcc indifferently, provoked a dose-dependent stimulation of gth secretion. cd2+ action was not altered by ad ... | 1990 | 1705429 |
| presence of four tachykinins in an acid extract of the carp intestinal bulb (cyprinus carpio). | 1. the pharmacological and chemical properties of substance p-like peptides isolated from an acid extract of the carp intestinal bulb were examined using guinea-pig ileum longitudinal smooth muscle. 2. on a sephadex g25 column (3 x 96 cm), smooth muscle contracting material was eluted as two peaks (fraction-1 and fraction-2). the molecular weight of the fraction-1 was estimated to be 2300 and that of the fraction-2 to be 1530. 3. the pharmacological properties of the contracting materials in fra ... | 1990 | 1705490 |
| distribution and migration pathways of hnk-1-immunoreactive neural crest cells in teleost fish embryos. | whole mounts and cross-sections of embryos from three species of teleost fish were immunostained with the hnk-1 monoclonal antibody, which recognizes an epitope on migrating neural crest cells. a similar distribution and migration was found in all three species. the crest cells in the head express the hnk-1 epitope after they have segregated from the neural keel. the truncal neural crest cells begin to express the epitope while they still reside in the dorsal region of the neural keel; this has ... | 1990 | 1706978 |
| tropomyosin from the striated muscles of carp (cyprinus carpio) and of icefish (channichthys rhinoceratus). | tropomyosin of fast-twitch, slow-twitch and cardiac muscles of carp and icefish has been isolated by hydroxyapatite chromatography. the subunit distribution has been investigated by polyacrylamide gel electrophoresis and by peptide mapping. the purified skeletal muscle tropomyosins all belong to the alpha family and differ from higher vertebrate tropomyosin by the lack of beta subunits. specific alpha isotypes are however encountered in fast-twitch fibres (alpha w subunit) and slow-twitch or int ... | 1990 | 1708998 |
| injection of rna from carp retina induces the formation of a membrane potassium channel in xenopus oocytes. | total rna was purified from freshly isolated retinas of adult carp and injected into oocytes of xenopus laevis (stages 5-6). two to six days after injection, depolarizing voltage-clamp steps evoked a slowly activated outward current as large as 3 microa. this current inactivated slowly with a single time constant (tau = 3.1 +/- 0.24 s.e.m., for vm = +30 mv). the current was inhibited by tetraethylammonium (3.8 mm for half-maximal inhibition). in the presence of co2+ (1 mm) or barium methanesulfo ... | 1991 | 1709045 |
| molecular location of a species-specific epitope on the hamster scrapie agent protein. | scrapie is a transmissible neurodegenerative disease of sheep and goats. an abnormal host protein, sp33-37, is the major protein component of the scrapie agent and the only known disease- or agent-specific macromolecule. two monoclonal antibodies (mabs), 4h8 (immunoglobulin g2b [igg2b]) and 6b11 (igg1), produced by immunizing mice with the intact hamster 263k scrapie agent protein, sp33-37ha, were found to have species specificity similar to that reported previously for mab 3f4 (igg2a), which wa ... | 1991 | 1710287 |
| color opponency in cone-driven horizontal cells in carp retina. aspecific pathways between cones and horizontal cells. | the spectral and dynamic properties of cone-driven horizontal cells in carp retina were evaluated with silent substitution stimuli and/or saturating background illumination. the aim of this study was to describe the wiring underlying the spectral sensitivity of these cells. we will present electrophysiological data that indicate that all cone-driven horizontal cell types receive input from all spectral cone types, and we will present evidence that all cone-driven horizontal cell types feedback t ... | 1991 | 1711573 |
| riboflavin carrier protein from carp (c. carpio) eggs: comparison with avian riboflavin carrier protein. | a protein exhibiting immunological cross-reactivity with the chicken egg-white riboflavin carrier protein was detected by radioimmunoassay in the eggs and serum of the fresh water fish cyprinus carpio and subsequently purified to homogeneity by use of affinity chromatography. fish riboflavin carrier protein resembled chicken riboflavin carrier protein with respect to most of its physicochemical characteristics. the major epitopes of chicken riboflavin carrier protein were shown to be conserved i ... | 1991 | 1713763 |
| cytoskeleton in microridges of the oral mucosal epithelium in the carp, cyprinus carpio. | microridges produce a characteristic fingerprint-like pattern on the surface of fish oral mucosa. the cytoskeleton in these microridges was examined by immunofluorescence microscopy and transmission electron microscopy after detergent extraction and decoration with myosin subfragment 1. the effect of cytochalasin b on microridges was probed with scanning electron microscopy. immunofluorescence microscopy revealed that actin filaments were present throughout the periphery of the epithelial cells ... | 1991 | 1714256 |
| an enzyme-linked immunosorbent assay for myelin basic protein-specific protein methylase i. | a sandwich enzyme-linked immunosorbent assay (elisa) has been developed to determine myelin basic protein (mbp)-specific protein methylase i. rabbit immunoglobulin anti-bovine mbp-specific protein methylase i, purified by sepharose-a affinity chromatography, was utilized as the primary antibodies, while the same antibodies which had been conjugated to peroxidase were employed as the indicator antibodies. this assay method was about 280 times more sensitive than the conventional trichloracetic ac ... | 1991 | 1715496 |
| excitatory responses to scyliorhinins i and ii in smooth muscle strips isolated from the carp intestinal bulb (cyprinus carpio). | 1. the effects of scyliorhinins i (scy i) and ii (scy ii) on longitudinal (lm) and circular muscle (cm) strips isolated from the carp intestinal bulb were investigated in vitro and compared with that of substance p (sp). 2. sp (0.3 nm-1 microm), scy i (0.3-300 nm) and scy ii (0.3 nm-1 microm) caused transient concentration-dependent contractions of lm strips. the ec50 values for sp, scy i and scy ii were 16 nm, 15 nm and 39 nm, respectively. tetrodotoxin and atropine partly decreased the contrac ... | 1991 | 1715522 |
| expression of two forms of carp gonadotropin alpha subunit in insect cells by recombinant baculovirus. | there are two types of cdna clones (designated alpha 1 and alpha 2) encoding the alpha subunit of carp gonadotropin. these two cdnas are derived from different genes and encode proteins that differ by seven amino acid residues (three in the signal peptide and four in the mature polypeptide). expression of these two cdnas in insect cells by recombinant baculovirus revealed that the alpha 1 subunit, after noncovalent association with the beta subunit, has the same potency as the native alpha subun ... | 1991 | 1715566 |
| similar properties of cgmp-activated channels between cones and rods in the carp retina. | using patch-clamp techniques, properties of cgmp-activated channel were studied at a single-channel level in order to examine (1) whether any differences are recognized between the cgmp-activated channels of rods and cones in the same animal species, and (2) whether the channel properties of the same photoreceptor class differ in different animal species. experiments were performed on inside-out membrane patches excised from outer segments of rods and morphological subtypes of cones in the carp ... | 1991 | 1715743 |
| plasmodium falciparum reinfection in children from a holoendemic area in relation to seroreactivities against oligopeptides from different malaria antigens. | the rate and densities of plasmodium falciparum reinfections were investigated in children five to 14 years old from one village in tanzania with a high transmission rate. initial parasitemias were eradicated by a curative treatment with quinine, a drug with a short elimination half-life, to minimize the effects of residual drug on reinfection. the seroreactivities to seven oligopeptides, representing t and b cell epitopes from the ring erythrocyte surface antigen (pf155/resa), the clustered arg ... | 1991 | 1718179 |
| glial fibrillary acidic protein in the fish optic nerve. | the intermediate filament glial fibrillary acidic protein (gfap) is the predominant cytoskeletal protein of mature glial cells in the mammalian nervous system. the nervous systems of lower vertebrates, such as fish, have been examined for the presence of gfap and several investigators have shown that goldfish (carassius auratus) brain contains gfap-positive astrocytes. the same studies have demonstrated that, in contrast to the brain, the optic nerve of goldfish did not show any gfap immunoreact ... | 1991 | 1718861 |
| biocytin: intracellular staining, dye-coupling and immunocytochemistry in carp retina. | correlation of electrophysiological and morphological, including ultrastructural, characteristics of neurones is important for understanding the functional organization of neuronal systems. further correlation with neurotransmitter content is essential for determining the neurochemical(s) used by a given neurone for propagating its signal. the two main neuronal markers presently available (lucifer yellow and horseradish peroxidase) are not satisfactory for correlating all three aspects. we have ... | 1991 | 1724386 |
| immunoreactive endothelin in plasma of nonmammalian vertebrates. | immunoreactive endothelin (ir-et) was estimated in plasma of nonmammalian vertebrates, using radioimmunoassay (ria) for endothelin-1 (et-1). blood samples were collected from unanesthetized animals. plasma et was determined by ria after extraction. plasma levels of ir-et were 0.7 +/- 0.2 pg/ml (n = 6) in the hagfish eptatretus burgeri, 4.3 +/- 0.9 pg/ml (n = 5) in the banded dogfish triakis scyllia, 3.6 +/- 0.8 pg/ml (n = 3) in the common japanese conger conger myriaster, 9.6 +/- 1.4 pg/ml (n = ... | 1991 | 1725396 |
| epitopes of the plasmodium falciparum clustered-asparagine-rich protein (carp) recognized by human t-cells and antibodies. | linear b- and t-cell epitopes have been identified in the plasmodium falciparum clustered-asparagine-rich-protein (carp). twenty-six synthetic peptides, 15-25 amino acids in length, were assayed for their ability to stimulate purified, human t-cells primed to p.falciparum by natural infection to proliferate and/or secrete gamma-interferon (ifn gamma). the plasma of malaria exposed individuals were tested for antibody reactivity with peptides coupled to bovine serum albumin in a semiquantitative ... | 1991 | 1725821 |
| voltage clamp study of electrophysiologically-identified horizontal cells in carp retina. | passive membrane properties and electromotive force of light modulated currents of l-, r/g-type and rod-driven horizontal cells were studied by voltage-clamp using double-barrelled micro-electrodes whilst perfusing with 5 microm dopamine to uncouple the gap junctions. input impedances of horizontal cells in darkness were 31 +/- 1.4 m omega (mean +/- se, n = 63); the resting potentials were -37 +/- 1.3 mv. current-voltage relationships had regions of both inward and outward rectification and a re ... | 1991 | 1726897 |
| purification and characterization of maturation-promoting factor in fish. | maturation-promoting factor (mpf) activity has been demonstrated for the first time in fish oocytes. we purified mpf from a 100,000g supernatant of crushed, naturally spawned carp oocytes using four chromatography columns: q-sepharose fast-flow, p13suc1-affinity sepharose, mono s, and superose 12. the final preparation was purified over 1000-fold with a recovery of about 1%. on superose 12, mpf eluted as a single peak with an apparent molecular weight of 100 kda. sds-page analysis of the active ... | 1992 | 1728595 |
| [the effect of the accident at the chernobyl atomic electric power station in 1986 on the fish population of a cooling pond]. | the cytogenetic analysis of cyprinus carpio eyes, developing blicca bjoerkna l. eggs, and eggs and larvae of hypophtalmichthys molitrix val. has demonstrated that the yield of cells with chromosome aberrations in the fish species under study is normal, while the level of variability of morphometric indices in offspring is considerably higher than that in parent fish. | 2006 | 1745759 |
| importance of the carg box in regulation of beta-actin-encoding genes. | the beta-actin-encoding gene (act) in carp is regulated by several cis-acting regulatory elements including the evolutionarily conserved cc(a/t)6gg (carg box or serum-response element) sequences positioned in the promoter region between the caat and tata boxes and in the first intron. to address the roles of the two carg boxes on gene expression, we replaced them with linker sequences. the carg box in the proximal promoter was not required for promoter activity in tissue-cultured cells, but was ... | 1991 | 1748307 |
| the use of the carp retina in neurobiology: its uniqueness and application for neural network analyses of the inner retina. | 1991 | 1758963 | |
| biochemical alterations in fish fingerlings (cyprinus carpio) exposed to sublethal concentration of linear alkyl benzene sulphonate. | fish fingerlings (cyprinus carpio) were exposed to sublethal concentrations of linear alkyl benzene sulphonate (0.005 ppm) for 24, 48, and 96 h under static laboratory conditions. alterations were noted in the levels of glycogen, lactic acid, sialic acid, and acid and alkaline phosphatases in the gill, liver, and kidney. this chemical may impair metabolic processes in fingerling carp, because increased exposure resulted in significant increases in lactic acid and decreases in glycogen and sialic ... | 1991 | 1759846 |
| scale and bone type i collagens of carp (cyprinus carpio). | 1. soluble type i collagens were isolated from the scale and bone (skull) of lathyritic carp. each tissue collagen was assumed to consist of two different molecular forms, (alpha 1)2 alpha 2 as a main component and alpha 1 alpha 2 alpha 3 as a minor one. 2. the possible coexistence of these two forms in soluble type i collagen of carp was previously observed for skin and muscle, but not for the swim bladder in which only the form of (alpha 1)2 alpha 2 was found. 3. these composite results sugges ... | 2006 | 1764927 |
| purification and characterization of a calsequestrin-like calcium-binding protein from carp (cyprinus carpio) sarcoplasmic reticulum. | 1. a calsequestrin-like calcium-binding protein was purified from carp sarcoplasmic reticulum by column chromatographies using deae-cellulose and butyl-toyopearl 650s. 2. the mol. wt was estimated to be 50 kda, which was larger than that of rabbit calsequestrin (42 kda). 3. carp calsequestrin-like protein bound ca2+ with a higher affinity (apparent kd = 400 microm) and lower capacity (25 mol/mol) compared with rabbit calsequestrin (1 mm and 40-50 mol/mol, respectively). 4. anti-carp calsequestri ... | 1991 | 1769203 |
| double mixing stopped-flow method for the study of equilibria and kinetics of dimer-tetramer association of hemoglobins: studies on hb carp, hb a, and hb rothschild. | a double mixing stopped-flow method is described for studying the dimer-tetramer equilibria of oxyhemoglobins and the kinetics of association of unliganded dimers. the three hemoglobins studied were: hb carp, hb a, and hb rothschild (trp beta 37 (c3)----arg). the new method reproduces the data obtained for oxyhb a by other established methods. in agreement with previous studies, the new method indicates little, if any, dissociation of oxyhb carp into dimers even in 2 m urea solutions (0.1 m bis- ... | 1991 | 1776671 |
| nutrition of aquaculture species. | dietary requirements for amino acids and fatty acids have been reported for channel catfish (ictalurus punctatus), salmonids (oncorhynchus spp.), common carp (cyprinus carpio), tilapias (oreochromis spp.), and eel (anguilla japonicus). most of the vitamin and mineral requirements are available for channel catfish and salmonids, and some are available for common carp, tilapia, eel, and other finfish and crustaceans. from this available information, cost-effective feeds can be formulated for the m ... | 1991 | 1778835 |
| physicochemical characterization of industrial effluents and their effect on fish survival. | water samples taken from selected industrial drainages and receiving streams in and around islamabad, pakistan, during the summers of 1987 to 1990 showed disturbances in ph and low oxygen levels. selected metals (fe, pb, zn, cd, cu, ni, hg) were higher in all effluents than in receiving waters. static bioassays of the undiluted industrial effluents from three sites caused 100% mortality in carp during the first 24 hrs. fishes also suffered 30 to 60% mortality when exposed to other undiluted indu ... | 1991 | 1779125 |
| treatment of cyprinus carpio l. and clarias gariepinus (burchell) embryos with formalin and malachite green: effect of concentration and length of treatment on their survival. | malachite green and formalin were investigated as prophylactic dip treatments for developing embryos of cyprinus carpio and clarias gariepinus. based on the findings, recommendations are made on the respective concentrations to be used and exposure periods to be followed for both formalin and malachite green for both fish species. | 1991 | 1780123 |
| investigations into the effects of concentration and duration of exposure to formalin and malachite green on the survival of the larvae and juveniles of the common carp cyprinus carpio l. and the sharptooth catfish clarias gariepinus (burchell). | prophylactic dip treatments using formalin and malachite green were applied to 4-day old larvae and 12- and 20-day old juveniles of the european common carp, cyprinus carpio and the african sharptooth catfish, clarias gariepinus. treatments consisted of 100 mg/l malachite green for exposure periods of 10, 30 or 90 s and 200 mg/l formalin, administered for 30, 60 or 90 min. larvae and juveniles of c. gariepinus could be treated with 100 mg/l malachite green for 10 s, or with 200 mg/l formalin for ... | 1991 | 1780124 |
| numbers of myosatellite cells in white axial muscle of growing fish: cyprinus carpio l. (teleostei). | by means of transmission electron microscopy (tem), the percentage of myosatellite cells was shown to decrease from about 6% in carp of 5 cm standard length (sl) to less than 1% in carp larger than 18 cm sl. the ratio between muscle nuclei and non muscle nuclei remained constant. these tem data, combined with data on the amount of dna per gram of muscle tissue and per nucleus, were used to calculate the numbers of myosatellite cells per gram of tissue (tem-dna method). total numbers of myosatell ... | 1991 | 1781451 |
| biosynthesis of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one in the ovaries of grey mullet (mugil cephalus) during induced ovulation by carp pituitary homogenates and an lhrh analogue. | three female mullets received a priming injection of carp pituitary homogenate followed by a resolving injection of an lhrh analogue 24 hr later. ovarian biopsies were obtained just prior to the first injection (phase i), 24 hr after the first injection (i.e., immediately before the second injection, phase ii), and 8 hr after the second injection (phase iii). two fish (nos. 1 and 3) spawned approximately 12 hr after the second injection. serum levels of testosterone increased to some extent duri ... | 2001 | 1783267 |
| effects of 17 beta-estradiol and carp gonadotropin on vitellogenesis in normal and hypophysectomized european silver female eel (anguilla anguilla l.) employing a homologous radioimmunoassay for vitellogenin. | the european silver eels are at the early stages of vitellogenesis before the marine reproductive migration. vitellogenesis was induced by 17 beta-estradiol (e2) alone and by a purified carp gonadotropin (cgth). we studied and compared their effects on plasma vitellogenin (vg) levels and ovarian yolk contents in female normal (n) and hypophysectomized (h) eels for both treatments. to this purpose an homologous radioimmunoassay (ria) was established. eel vg was purified to homogeneity on 0.1% sds ... | 1991 | 1783271 |
| neuropeptide y stimulates in vivo gonadotropin secretion in teleost fish. | the effects of the neuropeptide y (npy), alone or in combination with a gonadotropin-releasing hormone analogue, d-ala6-desgly10-pro9-net lhrh (lhrha), have been studied on the in vivo secretion of the maturational gonadotropin (gth2) in the rainbow trout oncorhynchus mykiss and the common carp cyprinus carpio. depending on the species, two routes of administration were used: in trout, intraperitoneal injection (20 micrograms/kg body wt); in carp, direct infusion (3 micrograms/kg body wt) into t ... | 1991 | 1783272 |
| effect of proteases on the activity of enolase from muscle of carp (cyprinus carpio) and pig. | the treatment of enolase from pig and carp (cyprinus carpio) with proteases resulted in a decrease of enzymatic activity, which depended on the kind of protease used. the most active were trypsin and subtilisin. substrate and magnesium ions protected enolase against inactivation. the enolase from pig muscle was much more resistant to protease action than this enzyme from carp muscle. some differences in the structure between the two enolases are suggested. | 1991 | 1796695 |
| dendritic contacts between neighboring homologous amacrine cells in carp retina. | 1991 | 1798608 | |
| conductance-decreasing synaptic mechanisms mediating cone input to h1 horizontal cells in carp retina. | 1991 | 1798613 | |
| the role of tubificid worms as an intermediate host in the life cycle of myxobolus pavlovskii (akhmerov, 1954). | myxobolus pavlovskii from the gills of silver carp (hypophthalmichthys molitrix) were used in attempts to transmit the infection under laboratory conditions. spores of m. pavlovskii were placed in aquaria that contained sterilized sand and had been filled with tap water at 15-16 degrees c. furthermore, oligochaetes (90% tubifex tubifex) were added and examined by wet mounts and histology. hexactinomyxon spores developed after about 3 months only in t. tubifex that had been exposed to m. pavlovsk ... | 1991 | 1805209 |
| in vitro study on the impact of fish sera on the survival and fine structure of the eel-pathogenic acanthocephalan paratenuisentis ambiguus. | the effects of fish sera on the growth and fine structure of infective larvae of the eel-pathogenic acanthocephalan paratenuisentis ambiguus (eoacanthocephala: tenuisentidae) were studied under in vitro conditions using sera from the final host anguilla anguilla and from two accidental fish hosts as well as fetal calf serum. as controls larvae were also kept in medium in the absence of serum and in experimentally infected eels. sera from the accidental fish hosts carp and rainbow trout exerted t ... | 1991 | 1805215 |
| 5 alpha-pregnane-3 beta, 7 alpha, 17,20 alpha- and -20 beta-tetrols as metabolites of progesterone and 17-hydroxyprogesterone in carp (cyprinus carpio) ovarian incubations. | 1991 | 1808020 | |
| development of khawia sinensis hsü, 1935 (cestoda: caryophyllidea) in the fish host. | the development of the tapeworm khawia sinensis has been observed up to the stage of sexually mature parasites releasing eggs in an experimentally infected definitive host (cyprinus carpio) at 15-16 degrees c. juvenile, maturing, adult and gravid tapeworms were found 2-12, 19-24, 36-62, and 78-91 days post infection, respectively. no apparent size difference between tapeworms from carp kept at 20-22 degrees c, examined 1-16 days post infection, and those from 15-16 degrees c was observed. in nat ... | 1991 | 1808032 |
| differentiation of eosinophilic granulocytes of carp (cyprinus carpio l.). | electron-microscopic studies were conducted to observe ultrastructural changes during differentiation of eosinophilic granulocytes in carp (cyprinus carpio l.). differentiation at the myelocyte stage was found to relate to specific granules made of dense and light fields. by maturation they assume a mosaic-like texture and in each granule of mature granulocytes, a light, central "internum" and a peripheral dense wrapper can be distinguished. the activity peroxidase and acid phosphatase is locate ... | 1991 | 1814414 |
| sublethal effects of textile dye stuff effluent on selected oxidative enzymes and tissue respiration of cyprinus carpio (linn.). | toxic effects of sublethal concentration of dye stuff effluent on succinic dehydrogenase (sdh) and lactic dehydrogenase (ldh) activities and tissue respiration were studied in c. carpio. while the sublethal exposure significantly reduced sdh activity and tissue respiration, ldh activity increased in gill, brain, liver, muscle and kidney. the maximum inhibition of sdh activity (74%) was recorded in gill and the minimum (38%) in liver. the percentage reduction of oxygen consumption in the tested t ... | 1991 | 1814839 |
| [elaboration of a dried-salted and smoked product using aquaculture species]. | the purpose of this work was to adapt the procedure developed at the instituto nacional de la nutrición salvador zubirán (innsz) for the conservation of the sardine to aquaculture species such as carp (ciprinus carpio) and tilapia (tilapia sp). the mixtures were designed on the basis of the chemical score method. the preparation of the patties included different fish presentations, and two methods were evaluated in order to obtain fish pulp. in the formulation of the final product, defatted soy, ... | 1991 | 1824516 |
| [trematodiases in tataria]. | high opisthorchiasis morbidity among local population and domestic animals was recorded in 1966 in alekseevskiĭ district of tataria. repeated screenings performed in 1989 revealed besides the above disease cases of pseudoamphistomiasis. 183 subjects have been examined, 90 of them (40.9%) revealed hepatobiliary system damages. bile analysis performed in 61 patients revealed lambliasis in 1 case, opisthorchiasis in 3 cases and pseudoamphistomiasis in 31 cases. metacercaria of the above trematode w ... | 1991 | 1829788 |
| carp erythrodermatitis (ce) due to an aeromonas hydrophila infection. casuistic and experimental results. | in november 1987 high losses of carp (cyprinus carpio) with the main symptom of skin ulcera were observed in a farm in northern greece. sixty-six isolates of bacteria, characterized mainly as aeromonas hydrophila or pseudomonas spp. could be isolated from lesions of diseased fish. transmission experiments with these isolates using mirror carp showed that aeromonas hydrophila strains induced identical clinical and pathological pictures after intra- or subcutaneous injection. extracts of these aer ... | 1991 | 1830440 |
| identification of immunoreactive atrial natriuretic peptide in bulbus arteriosus of freshwater teleostean fish. | 1. the presence and partial characterization of immunoreactive atrial natriuretic peptide (ir-anp) in the bulbus arteriosus were investigated using high performance liquid chromatography (hplc) and radioimmunoassay (ria) in several freshwater teleostean fish (catfish, silver crusian carp, snakehead, and rice eel). 2. the level of ir-anp in the bulbus arteriosus was comparable with that in the atria at about 5-15 pg/mg, 6-13 pg/mg wet wt, respectively, but was 2-4-fold higher than that in the ven ... | 1991 | 1839978 |
| gamma-crystallin genes in carp: cloning and characterization. | the carp gamma-crystallin gene family was found to be composed of at least three members: gamma m1, gamma m2 and gamma m3. the encoded products are very similar to other known gamma-crystallins but with their own peculiarities: (1) they all have a high methionine content: 12.4%, 14% and 8.4% in gamma m1, gamma m2 and gamma m3, respectively; and (2) the amino acid sequences are aberrant in the region before connecting peptides and its corresponding region in motif 4. their protein structures migh ... | 1991 | 1840497 |
| ecological aspects of nematode parasites of introduced salmonids from valdivia river basin, chile. | between 1986 and 1987, 666 fishes distributed among the following species introduced in chile, and from different sectors of the valdivia river basin (39 degrees 30'-40 degrees 00's, 73 degrees 30'-71 degrees 45'w), were examined: 348 salmo trutta, 242 salmo gairdneri, 24 cyprinus carpio and 52 gambusia affinis holbrooki. the presence of camallanus corderoi and contracaecum sp. in s. gairdneri and of c. corderoi in s. trutta is recorded in chile for the first time. cyprinus carpio and g. a. holb ... | 2006 | 1842391 |
| retinal morphology of cyprinid fishes: a quantitative histological study of ontogenetic changes and interspecific variation. | morphological patterns of the retina, cone size and density, rod density, rod-cone ratio, ganglion cell density, convergence of receptor cells, resolving power (rp) and regionalization were examined throughout life history in roach and in adults of asp, bream, common carp, roach and sabre carp. the retina of hatchlings is packed with small cones. during larval and juvenile growth the retina stretches, cones increase in diameter and rods are present in increasing numbers. photopic and scotopic se ... | 1991 | 1843750 |
| studies on rod horizontal cell s-potential in dependence of the dark/light adapted state: a comparative study in cyprinus carpio and scyliorhinus canicula retinas. | changes in light sensitivity, response time-course and electrical coupling of rod driven horizontal cells (rhc) were examined in the dark and light adapted states by intracellular recording. comparative analyses were made of two fishes from different photic environments. rhc of cyprinus carpio and external horizontal cells (ehc) of scyliorhinus canicula retinas showed similar changes of their electrical responses to light stimuli when the dark-adapted retina was light-adapted: a decrease in sens ... | 1991 | 1843752 |
| dendritic morphology of a class of interstitial and normally placed amacrine cells revealed by intracellular lucifer yellow injection in carp retina. | the dendritic morphology of a class of interstitial amacrine (isa) and normally placed amacrine cells was investigated in carp retina. we first identified their fluorescent nuclei after preloading with 4,6-diamidino-2-phenylindole (dapi) in living or aldehyde-fixed retinal wholemounts and then injected them iontophoretically with lucifer yellow (ly) under microscopic control. although dapi appeared to be accumulated nonspecifically by amacrine and ganglion cells, isa cell nuclei were discriminat ... | 1991 | 1843753 |
| ultraviolet photoreception in carp: microspectrophotometry and behaviorally determined action spectra. | this study demonstrates correlations between u.v. sensitivity and microspectrophotometric absorption spectra determined sequentially for the same group of individuals. we used the heart-rate conditioning technique to measure spectral sensitivity of carp, a species known to have u.v.-sensitive photoreceptors. mean spectral sensitivity (n = 3) determined with a spectrally-broad background (450 nm long pass filter) revealed a small but consistent u.v. peak (lambda max of 380 nm) in addition to the ... | 1991 | 1843761 |
| the existence of 25-hydroxyvitamin d3-1 alpha-hydroxylase in the liver of carp and bastard halibut. | we have found that carp and bastard halibut contain 25-hydroxyvitamin d3 (25-d3)-1 alpha-hydroxylase in the liver besides in the kidney by the following in vivo and in vitro experiments. when [3h]-25-d3 was intraperitoneally injected to vitamin d(d)-deficient carp and normal bastard halibut (d-deficient bastard halibut could not be raised because they died during farming), the profiles of high-performance liquid chromatography (hplc) of the plasma lipid extract showed the formation of a peak cor ... | 1991 | 1846929 |
| calcium channels from cyprinus carpio skeletal muscle. | the complete amino acid sequence of the l-type calcium channel alpha 1 subunit from the carp (cyprinus carpio) white skeletal muscle was deduced by cdna cloning and sequence analysis. the open reading frame encodes 1852 amino acids (mr 210,060). a 155-amino acid cooh-terminal sequence (after the fourth internal repeat) is evolutionarily preserved (90% homology) and may represent an important functional domain of l-type calcium channels. the photolabeled, membrane-bound, and purified carp alpha 1 ... | 1991 | 1846962 |
| [the immunological status of cyprinus carpio l. in experimental mycotoxicosis]. | some humoral factors of nonspecific resistance have been determined in carp yearlings in 28 days after intraperitoneal administration of fungi culture filtrate (i), conidia (ii), micellar extract (iii) of the fungus aspergillus fumigatus fres., physiological solution (control). the toxicogenic and immunogenic effect of the fungus has been shown in i and ii variants of the experiment respectively. the injection of fungus micella did not have any influence on the studied factors. the account of sp ... | 1991 | 1859857 |
| inactivation of enolase from carp (cyprinus carpio) muscle by 2,3-butanedione. | enolase from white muscle of carp (cyprinus carpio) is inactivated by 2,3-butanedione in borate buffer. magnesium ions as well as substrate--2-phosphoglycerate markedly altered the rate and extent of inactivation. the partially inactivated enzyme shows unaltered km but decreased vmax after 10 min incubation with butanedione, however after 60 min incubation the km value increased 2.5 fold. | 1991 | 1863278 |
| absorption of bioactive human growth hormone after oral administration in the common carp (cyprinus carpio) and its enhancement by deoxycholate. | recombinant human growth hormone was administered orally to carp and serum levels of absorbed bioactive hormone were investigated using a highly sensitive nb2 rat lymphoma cell bioassay and radioimmunoassay. serum levels of bioactive hgh reached maximum values 30 min after oral intubation and then gradually decreased. co-administration of the hormone with deoxycholate to fasted carp resulted in up to a 1000-fold increase in absorption compared to aqueous solutions of the hormone, but had no effe ... | 1991 | 1869695 |
| pituitary and plasma arginine vasotocin levels in teleost fish. | the role of arginine vasotocin (avt) in teleostean osmoregulation is unclear. its administration may produce dose-dependent effects on renal fluid loss though the physiological significance of such effects is unknown due to the paucity of data on the normal range in pituitary and plasma avt levels. the present study addresses these questions by examining the pituitary avt activity in the flounder and plasma avt concentrations in the flounder, trout, and carp following alterations in environmenta ... | 1991 | 1879673 |