Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the somatic envelopes around the germ-line cells of polytrophic insect follicles: structural and functional aspects. | the polytrophic ovarioles of three insect species, the fruit fly drosophila melanogaster, the fungus gnat bradysia tritici, and the honeybee apis mellifera, were compared morphologically and with respect to the cytological organization of the peripheral somatic layers. staining with rhodaminyl-phalloidin revealed differences in the organization of the muscle strands of the epithelial sheath and the microfilament pattern in the basal part of the follicular epithelium (mid-vitellogenic stages). al ... | 1991 | 1796455 |
| [the effect of zootoxins on the formation of delayed hypersensitivity]. | the effect of bee venom (0.05 and 0.5 mg/kg) and the venom of vipera lebetina (0.05 and 0.5 mg/kg) on the reaction of delayed hypersensitivity was studied in experiments on cba mice. the character and trend of the effect was determined by the zootoxin species. the venom of vipera lebetina completely suppressed the cell immune reaction in a dose of 0.5 mg/kg and produced an adjuvant effect in a dose of 0.05 mg/kg. bee venom (0.5 mg/kg) failed to cause a suppressive effect and in a small dose (0.0 ... | 2006 | 1798644 |
| [total amitraz residues in bee honeys]. | a total of 330 bee honey samples was analysed in 1986-1990, and the results are reported. analysis was performed according to the method for the amitraz total residue determination by hydrolysis and steam distillation as described. 60% of the honeys were practically not contamined (total residue content amounting to 0.01 mg/kg), 8.5% of the honeys contained more than 0.05 mg/kg. maximal values of 0.2-0.5 mg/kg were stated. 54% of the rape-honeys contained more than 0.01 mg/kg, 19% more than 0.05 ... | 1991 | 1800907 |
| pharmacologic characterization of muscarinic receptors of insect brains. | muscarinic receptors in brain membranes from honey bees, houseflies, and the american cockroach were identified by their specific binding of the non-selective muscarinic receptor antagonist [3h]quinuclidinyl benzilate ([3h]qnb) and the displacement of this binding by agonists as well as subtype-selective antagonists, using filtration assays. the binding parameters, obtained from scatchard analysis, indicated that insect muscarinic receptors, like those of mammalian brains, had high affinities fo ... | 1991 | 1802028 |
| [the use of products from bee raising in ophthalmology and otorhinolaryngology]. | 1991 | 1803198 | |
| [kinetic study of mixed alpha glucosidase activity induced in honeybee hemolymph by in vivo injection of trehalose]. | in vivo injection, into emerging bees, of a quantity of trehalose sufficient to double the initial natural haemolymph level of the sugar, leads first to a reduction in the maximal velocity (vm) of the alpha-glucosidase activity, after 2 hours, followed by an increase in vm at 6 hours, then a return to normal. the substrate constant (kr) shows minor and insignificant fluctuations about the initial value, and the hill coefficient (h) a slight, but significant, rise 2 h and 8 h after injection. the ... | 1991 | 1806194 |
| cutaneous reaction induced by retained bee stinger. | the clinical features and histopathologic findings of a 54-year-old korean male who had retained the sting apparatus of a bee for four months are described. the clinical features showed ulcerative, erythematous plaques with irregular borders which resembled cutaneous neoplasms such as squamous cell carcinoma. histopathologic findings included epidermal necrosis and marked pseudoepitheliomatous hyperplasia. in the dermis, the stick-shaped sting apparatus of the bee was demonstrated and intense ly ... | 1991 | 1806605 |
| immunoblot analysis of ige and igg antibodies to honey bee venom: cross sectional and sequential studies in bee sensitive subjects. | to investigate the specific ige and igg immune response to honey bee venom (bv), we performed immunoblot analysis of sera from 47 bee sensitive subjects and followed the response during and after venom immunotherapy in 15 of these subjects. fifteen venom proteins varying in molecular size from 20 to 105 kda were identified as being antigenic and consisted of a high molecular weight (hmw) group (5 to 105 kda, containing the previously identified allergens b and c) and a low molecular weight group ... | 1991 | 1807261 |
| [study of hormone replacement therapy following total thyroidectomy in thyroid cancer--with special reference to the analysis of thyroid hormone peripheral effects, using indirect calorimetry]. | peripheral effects of thyroid hormones were examined using an indirect calorimetry in 18 patients with thyroid cancer before and after total thyroidectomy. peripheral effects of exogenous thyroid hormones in tsh-suppression therapy after thyroidectomy were also studied. the subjects were maintained without hormone replacement for 3 weeks after total thyroidectomy. the ratio of resting energy expenditure to basal energy expenditure (ree/bee) was determined before operation, before hormone replace ... | 1991 | 1809878 |
| metabolic signaling between photoreceptors and glial cells in the retina of the drone (apis mellifera). | experimental evidence showing metabolic interaction and signaling between photoreceptors-neurons and glial cells of the honeybee drone retina is presented. in this tissue [3h]2-deoxyglucose ([3h]2dg) in the dark and during repetitive light stimulation is phosphorylated to [3h]2-deoxyglucose-6p ([3h]2dg-6p) almost exclusively in the glial cells. hence, stimulus-induced changes in the rate of formation of [3h]2dg-6p occurs predominantly in the glial cells. repetitive stimulation of the photorecept ... | 1991 | 1815828 |
| glycerol-3-phosphate dehydrogenase isozyme variation in adult meliponids (hymenoptera: apidae). | glycerol-3-phosphate dehydrogenase (g-3-pdh) isozymes were investigated in several bee and wasp species to verify if variations detected in g-3-pdh-2 isozymes are closely related to the age and activity of adult workers in the nest or hive of social species. in the solitary, the semisocial, and one social bee species, no phenotypic variations were detected for g-3-pdh-2 isozymes, and this was also the case for all wasp species investigated which were characterized as social. these results allow ... | 1991 | 1820023 |
| expression of honeybee prepromelittin as a fusion protein in escherichia coli. | strategies for the expression of precursors of eukaryotic secreted proteins as part of fused proteins in escherichia coli have been explored. a fusion protein with beta-galactosidase at the n-terminal end and honeybee prepromelittin at the c-terminal end (beta-gal-pm) was expressed in low amounts as a cleaved polypeptide, from which the promelittin portion had been removed. inclusion in the induction culture of 10 mm mgcl2 or 8.3% (v/v) ethanol, inhibitors of signal peptidase, gave rise to the f ... | 1991 | 1821810 |
| [mites (acarida) of honey bee (apis mellifera l.) in poland]. | 400 samples of natural winter debris collected from bee hives, 150 samples of stored honey and 100 samples of pollen collected by bees were examined; full of food and empty honey combs, brood and adult bees were also observed. 100% of samples of debris, 90% of pollen and almost 24% of honey samples contained mites; they were found also on honey combs and on died and living bees (brood, imagines). 33 mite species were found. besides of parasite varroa jacobsoni oud. numerous mites belonging to ac ... | 1991 | 1823503 |
| peculiarities in ontogenesis and reproductive cycle of the mite varroa jacobsoni (parasitiformes, varroidae) and its relation to the host apis mellifera. | on the basis on functional morphology and ecology of varroa jacobsoni the reproductive cycle of female mites was divided into 5 stages: copulation, puberty, preoviposition, oviposition, rehabilitation. the whole ontogeny from egg to formed protonymph in the chorion proceeds very intensively (26 to 30 hours). besides the reproductive potential is realized during an extremely short period (6 to 7 days) which duration is determined by the total duration of the mite's ontogeny, so that the developme ... | 1991 | 1823505 |
| bee sting optic neuritis. a case report with visual evoked potentials. | simultaneous recordings of the pattern visual evoked potential and the pattern electroretinogram were recorded in a case of unilateral optic neuritis following a bee sting on the eye. the patient presented with severe eye pain, apoplectic visual loss, acute optic disc swelling and minimal signs of ocular inflammation and eyelid edema. the vision failed to recover. the electrophysiological recordings initially showed a delay in the p100 wave of the pattern visual evoked potential. on follow-up, a ... | 1991 | 1827460 |
| nucleotide sequence of a middle repetitive dna from honey bees. | the nucleotide sequence of a 264-bp clone, pafr3.4, which represents a related, tandemly arrayed, middle repetitive dna family within various subspecies of the honey bee (apis mellifera l.), has been determined. | 1991 | 1840551 |
| acute macular neuroretinopathy after shock. | acute macular neuroretinopathy is a rare disease that has been described mainly in women taking hormonal contraceptives. an association either with a viral illness or with the parenteral use of sympathomimetics was sometimes found. we describe its occurrence in a 22-year-old female following an anaphylactic shock after a bee sting, and in a 26-year-old female following a pregnancy complicated with vena cava syndrome and delivery by caesarean section. a combination of factors including hypoperfus ... | 1991 | 1841003 |
| membrane interactions of amphiphilic polypeptides mastoparan, melittin, polymyxin b, and cardiotoxin. differential inhibition of protein kinase c, ca2+/calmodulin-dependent protein kinase ii and synaptosomal membrane na,k-atpase, and na+ pump and differentiation of hl60 cells. | interactions of certain naturally occurring, amphiphilic polypeptides with membranes were investigated. mastoparan (wasp venom toxin), melittin (bee venom toxin), cardiotoxin (cobra venom toxin), and polymyxin b (antibacterial antibiotic) inhibited protein kinase c stimulated by phosphatidylserine bilayer or arachidonate monomer and blocked binding of [3h] phorbol 12,13-dibutyrate to protein kinase c in the presence of phosphatidylserine bilayer, with ic50 values (concentrations causing 50% inhi ... | 1991 | 1847132 |
| [advice and handy drugs can be sufficient in bee and wasp venom allergy]. | 1991 | 1857153 | |
| learning by honeybees (apis mellifera) on arrival at and departure from a feeding place. | the question of when in the course of a visit to a feeding place foraging honeybees (apis mellifera) learn about its location was studied in experiment 1 by moving the animals a short distance after they arrived and began to feed. a preference for the arrival place developed, although less rapidly than in control animals for which the arrival and departure places were the same. in experiments 2-5, a distinctive object was used to define the location of the feeding place. when the object was remo ... | 1991 | 1860311 |
| genetic differences in learning behavior in honeybees (apis mellifera capensis). | workers from colonies of cape honeybees show marked phenotypic differences in performance in proboscis extension reflex (per) conditioning. analysis of these differences using parthenogenetic offspring groups permitted the estimation of genotypic values and revealed a high degree of genetic variability that is evident among related as well as unrelated bees. the results obtained from related groups are of particular importance, since they demonstrated the existence of strong genetic variability ... | 1991 | 1863260 |
| gdp-fucose: beta-n-acetylglucosamine (fuc to (fuc alpha 1----6glcnac)-asn-peptide)alpha 1----3-fucosyltransferase activity in honeybee (apis mellifica) venom glands. the difucosylation of asparagine-bound n-acetylglucosamine. | incubation of honeybee (apis mellifica) venom-gland extracts with gdp-[14c]fucose and glcnac beta 1----2man alpha 1----6(glcnac beta 1----2man alpha 1----3)man beta 1----4glcnac beta 1----4(fuc alpha 1----6)glcnac beta 1----n-asn-peptide(nac) gave a labeled product in 40% yield. analysis by 500-mhz 1h-nmr spectroscopy indicated the transferred fucose-(fuc) residue to be alpha 1----3-linked to the asn-bound glcnac. further proof was provided by one-dimensional and two-dimensional 1h-nmr analysis ... | 1991 | 1868856 |
| multiplication and persistence of spiroplasma melliferum strain a56 in experimentally infected suckling mice. | strain a56 of the bee pathogen spiroplasma melliferum was isolated from a honeybee (apis mellifera) during ecological studies on mosquito spiroplasmas in savoie (france). when inoculated intracerebrally (i.c.) into 48-h old suckling mice, this strain was found to replicate to very high titres and to persist in brain for up to 9 months in one individual. we attempted to increase the "neurotropism" of a56 by sequential i.c. passages. during the first two passages, multiplication of the organisms w ... | 1991 | 1871426 |
| bee venom anti-idiotypic antibody is associated with protection in beekeepers and bee sting-sensitive patients receiving immunotherapy against allergic reactions. | bee venom (bv) anti-idiotypic (anti-id) antibodies (abs) were studied in nonreactive beekeepers, patients receiving bv immunotherapy (it), and in patients with bee-sting hypersensitivity. detection of serum anti-bv was determined either by the phadebas rast test for ige and igg concentrations or by isoelectric focusing followed by capillary blotting onto nitrocellulose membranes. clonotypic analyses of ab were made with specific probes for bv or bv anti-id; 13/14 nonreactive multiple-sting beeke ... | 1991 | 1880320 |
| pore-forming peptide of pathogenic entamoeba histolytica. | a polypeptide that causes pore formation in target-cell membranes is implicated in the potent cytolytic activity of pathogenic entamoeba histolytica. pore-forming material was purified to apparent homogeneity by a multistep procedure, and its analysis by nadodso4/page revealed one peptide of 4-5 kda under nonreducing or under reducing conditions. pore-forming activity was measured by depolarization of liposome membrane potential and was found to be optimally expressed at low ph. active material ... | 1991 | 1881907 |
| inhibition of venom phospholipases a2 by manoalide and manoalogue. stoichiometry of incorporation. | we have previously described the irreversible inhibition of cobra venom phospholipase a2 (pla2) by the marine natural product manoalide (mld) (lombardo, d., and dennis, e. a. (1985) j. biol. chem. 260, 7234-7240) and by its synthetic analog, manoalogue (mlg) (reynolds l. j., morgan, b. p., hite, g. a., mihelich, e. d., and dennis, e. a. (1988) j. am. chem. soc. 110, 5172-5177). we have now made a direct comparison of the action of these two inhibitors on pla2 from cobra, bee, and rattlesnake ven ... | 2007 | 1885583 |
| color opponent coding in the visual system of the honeybee. | a model is presented for the color vision system of the honeybee, which takes the nonlinear phototransduction process in the photoreceptors into account and assumes linear computations of the excitations of the photoreceptors. the model parameters are derived by a least squares fit of the scale values determined by multidimensional scaling analysis of the results of color choice experiments to the excitation values of two hypothetical spectral antagonistic coding cells. the psychophysical scale ... | 1991 | 1891826 |
| a method for estimating the minimum visual stimulus that evokes a behavioural response in the drone, apis mellifera male. | drones, attracted to an observation site by a scented lure, left the lure to fly directly towards a small object suspended nearby. an object the size of a queen bee was detected at distances up to 2 m. it is estimated that at this maximum distance the object will have decreased the light incident on a single rhabdom by 6%. | 1991 | 1891832 |
| assessment of energy expenditure by indirect calorimetry in healthy subjects and patients with liver cirrhosis. | the reliability of resting energy expenditure (ree) measurements by indirect calorimetry with a ventilated hood was investigated in 50 healthy controls and 10 patients with liver cirrhosis. in each subject basal energy expenditure (bee) was determined once and ree three times (morning ree1, noon ree2, afternoon ree3). in controls and patients the first 5-minute bee and first 5-minute ree (controls also second 5-minute ree) were higher than in the remainder of the 30-minute recording. only the la ... | 2017 | 1895487 |
| [studies on detection of serum iga and igg antibodies specific for chlamydia trachomatis in latent infections in males]. | we determined the serum concentrations of iga and igg antibodies specific for chlamydia trachomatis (c. trachomatis) by an indirect immunoperoxidase assay (ipazyme kit, savyon diagnostics, ltd., bee, shova, israel) to evaluate their diagnostic significance in latent infections in males. forty-five asymptomatic males whose wife or partner was suspected to be infected with c. trachomatis were studied and the incidence of serum iga (titer greater than or equal to 16) and igg (titer greater than or ... | 1991 | 1895621 |
| clinical characteristics of patients with repeated systemic reactions during specific immunotherapy with hymenoptera venoms. a retrospective study. | in general, specific immunotherapy with hymenoptera venoms can be considered as safe, but occasionally there are patients who cannot reach the maintenance dose due to repeated systemic reactions (rsr) or who suffer from rsr during maintenance therapy. in a multicenter retrospective study comprising seven departments in germany, austria and switzerland 23 patients with rsr were reported from approximately 3000 patients treated with hymenoptera venoms (bee and wasp venom to approximately equivalen ... | 1991 | 1897686 |
| comparison of the catalytic properties of phospholipase a2 from pancreas and venom using a continuous fluorescence displacement assay. | phospholipases a2 from pig pancreas and the venoms from bee, naja naja and crotalus atrox have been studied by using a new continuous fluorescence displacement assay that utilizes normal phospholipid substrates [wilton (1990) biochem. j. 266, 435-439]. with limiting amounts of substrate, the assay demonstrated stoichiometric conversion into products with both pancreatic and venom enzymes, and thus would allow phospholipid determination at concentrations down to about 0.1 microm. the substrate sp ... | 1991 | 1898370 |
| divalent cation-sensitive pores formed by natural and synthetic melittin and by triton x-100. | leakage of ions and low-molecular-weight metabolites from lettre cells is induced by synthetic melittin, as effectively as by melittin isolated from bee venom; in each case leakage is inhibited by ca2+, zn2+ or h+. inhibition of leakage by divalent cations is reversible in that lettre cells incubated with melittin (or with triton x-100) in the presence of inhibitory amounts of zn2+, when freed of zn2+ by egta or by centrifugation, begin to leak (in zn2(+)-sensitive manner). electrorotation of le ... | 1991 | 1899800 |
| direct gas chromatographic determination of carbaryl residues in honeybees (apis mellifera l.) using a nitrogen-phosphorus detector with confirmation by formation of a chemical derivative. | 1991 | 1903992 | |
| oral nutrition in patients receiving home cyclic parenteral nutrition: pattern of substrate utilization. | patients receiving cyclic home parenteral nutrition (pn) often have a significant oral caloric intake. this study describes the metabolic use of fuels, as assessed by indirect calorimetry, in eight stable, ambulatory, noncancerous, adult patients receiving glucose-based pn with (pn + oral) or without (pn only) a self-selected oral intake. patients' weight was 91 +/- 2% (mean +/- se) of ideal body weight, and fat mass was 22 +/- 5 and 31 +/- 2% of actual body weight in males and females, respecti ... | 1991 | 1908632 |
| mast cell degranulating (mcd) peptide and its optical isomer activate gtp binding protein in rat mast cells. | the mcd peptide in bee venom induces degranulation in mast cells. the internal calcium concentration of mast cells increased and remained high following mcd stimulation. this calcium increase was blocked by pertussis toxin (ptx) treatment, suggesting that mcd peptide activates ptx-sensitive g-protein. even in the absence of external calcium in the incubation medium, the calcium concentration increased by mcd treatment, but soon returned to the original level. d-mcd, the optical isomer of the mcd ... | 1991 | 1908786 |
| prospective randomized evaluation of two regimens for converting from continuous to intermittent feedings in patients with feeding gastrostomies. | forty enterally fed male patients were randomized to one of two regimens designed to determine the better means of converting them from continuous to intermittent enteral feedings. all patients received a nutritionally complete iso-osmolal 1 kcal/cc formula containing 6 g of nitrogen/l beginning on the second postgastrostomy day. half of the patients (20) were randomized to a discontinuous regimen abruptly changing from continuous to gradually increasing intermittent feedings until reaching thei ... | 2017 | 1910103 |
| kinetic characterization of phospholipase a2 modified by manoalogue. | manoalogue, a synthetic analogue of the sea sponge-derived manoalide, has been previously shown to partially inactivate the phospholipase a2 from cobra venom (reynolds, l. j., morgan, b. p., hite, e. d., mihelich, e. d., & dennis, e. a. (1988) j. am. chem. soc. 110, 5172) by reacting with enzyme lysine residues. in the present study, the inactivation of the phospholipases a2 from pig pancreas, bee venom, and cobra (naja naja naja) venom by manoalogue was studied in detail. manoalogue-treated enz ... | 1991 | 1911741 |
| effective charge of melittin upon interaction with popc vesicles. | the binding of bee venom melittin to small unilamellar vesicles and large nonsonicated multilamellar bilayer membranes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (popc) was studied by means of circular dichroism, 31p-nmr and electrophoretic mobility. the melittin binding isotherm for small unilamellar vesicles (suv) could be described by a partition equilibrium with kp = (6 +/- 1).10(4) m-1. electrostatic effects were taken into account by means of the gouy-chapman theory. comb ... | 1991 | 1911831 |
| differential response of apis mellifera acetylcholinesterase towards pirimicarb. | the kinetic analysis of apis mellifera acetylcholinesterase inhibition by the carbamate pirimicarb showed that native and detergent-solubilized membrane enzyme exhibited slightly different carbamylation kinetics. the acetylcholinesterase form sensitive to phosphatidylinositol-specific phospholipase c (pi-plc) was carbamylated more rapidly (kapp = 36.4 x 10(-3) min-1) than the pi-plc-resistant counterpart (kapp = 10.13 x 10(-3) min-1) which had a behavior close to that of the soluble tryptic enzy ... | 1991 | 1912459 |
| effect of apamin, a toxin that inhibits ca(2+)-dependent k+ channels, on learning and memory processes. | apamin, a neurotoxin extracted from bee venom, specifically binds to a particular class of ca(2+)-activated k+ channels which are involved in the slow afterhyperpolarization (s-ahp) that follows action potentials in many excitable cells. we tested in mice the effects of apamin on learning and memory processes. the results showed that pre-training injection of apamin accelerated the acquisition of a bar-pressing response but also increased the bar-pressing rates of the animals. this latter result ... | 1991 | 1913161 |
| an improved test for africanized honeybee mitochondrial dna. | mitochondrial dna derived from apis mellifera scutellata, the ancestor of the africanized bees of the new world, lacks a bglii restriction site found in other types of honeybee. we present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. digestion of the amplified product with bglii yields contrasting patterns between africanized and other honeybees. | 1991 | 1915781 |
| novel proliferative effect of phospholipase a2 in swiss 3t3 cells via specific binding site. | phospholipase a2 (pla2), ec 3.1.1.4, which catalyzes the release of free fatty acids from the sn-2 position of glycerophospholipids, has been extensively studied from the viewpoint of eicosanoid production (arita, h., nakano, t., and hanasaki, k. (1989) prog. lipid res. 28, 273-301). several lines of evidence suggest that extracellular pla2 is pathophysiologically related to some disorders, including inflammation and hypersensitivity. despite this, little is known of the precise mechanism of the ... | 1991 | 1918029 |
| common projection areas of antennal and visual pathways in the honeybee brain, apis mellifera. | the convergence of primary sensory neurons of the antennae, higher order visual interneurons, and antennal motoneurons was analysed with neuroanatomical techniques in the honeybee, apis mellifera. the different modalities evoke specific antennal responses in this insect. three different fluorescent dyes were applied successively in the same preparation in order to visualise the various fiber projections from the antennae and the lobula in the brain of the honeybee. three neuropile areas where se ... | 1991 | 1918441 |
| two-step inactivation of bee venom phospholipase a2 by scalaradial. | scalaradial (sld), a marine natural product isolated from the sponge (cacospongia sp., possesses anti-inflammatory properties in vivo and in vitro (pharmacologist 32: 168, 1990). in this study we characterize its effects against bee venom phospholipase a2 (pla2; ec 3.1.1.4). sld is a potent inactivator of bee venom pla2 with an ic50 value of 0.07 microm. inactivation of bee venom pla2 occurred in a time-dependent, irreversible manner. the rate of inactivation followed first-order reaction kineti ... | 1991 | 1930288 |
| appearance of macrophage migration inhibition factor in patients with systemic reactions to bee venom. | cellular responses in bee venom (bv) allergy is a controversial issue. previous studies could not reach an agreement whether this mechanism is activated as a result of allergic sensitization to bee venom. all previous works have used lymphocyte proliferation as their method to analyze cell-mediated immunity. in the present work, we tried to explore whether the production of macrophage migration inhibition factor (mif), which is another in vitro correlate with cellular responses, is increased in ... | 1991 | 1937921 |
| [cold-induced pathology at high altitude]. | cold injury is an objective danger in mountain climbing as well as in many outdoor and recreational sports such as skiing, fishing, etc. symptoms are easily recognizable by the experienced, and prevention is mostly possible. cold injury should be divided by pathological means in general hypothermia and local frostbite injuries. life-threatening deep hypothermia with coma and insufficient circulation or cardiac arrest is reversible under the condition of sufficient core rewarming, for instance by ... | 1991 | 1957091 |
| oligonucleotide dna fingerprinting discriminates super- and half-sisters in honeybee colonies (apis mellifera l.). | 1991 | 1961253 | |
| apamin binding sites in human c6 cell line and their accessibility to monoclonal antibodies. | apamin, a 18-amino acid bee venom toxic peptide specifically blocks a class of ca(2+)-activated k+ channels. i) mono 125i-iodoapamin binds to receptor sites in a human neuroglial cell line (c6 line) but not in human cell lines from pancreatic (rin5f line) and colonic origin (ht29 line). ii) receptor-bound apamin is still accessible to a large molecule since some anti-apamin monoclonal antibodies recognize apamin when bound to its receptor, both in intact cells of the human c6 glioma line and in ... | 1990 | 1966607 |
| evidence for leukotrienes in animal venoms. | to elucidate further the pathomechanisms of cutaneous whealing in response to insect or sea-animal stings, commercial sources of bee and wasp venoms and of sea nettle nematocyst extracts, as well as crude bee and wasp venoms, were examined for the presence of histamine, leukotrienes (lt) c4, ltb4 (radioimmunoassay and reverse-phase high-pressure liquid chromatography), and neutrophil chemotactic activity (nca). histamine was present in all samples with the highest levels in the crude insect veno ... | 1990 | 1968071 |
| parental analysis of introgressive hybridization between african and european honeybees using nuclear dna rflps. | african honeybees, introduced into brazil 33 years ago, have spread through most of south and central america and have largely replaced the extant european bees. due to a paucity of genetic markers, genetic interactions between european and african bees are not well understood. three restriction fragment length polymorphisms (rflps), detected with random, nuclear dna probes, are described. the polymorphisms are specific to bees of european descent, possibly specific to certain european races. ea ... | 1990 | 1974226 |
| an hla-associated nonresponsiveness to mellitin: a component of bee venom. | previous work has demonstrated a close association between certain histocompatibility antigens and the gene that controls the ige response to certain ragweed allergens. for example, there is a 90% association between ige production to the short ragweed allergen, amb a v, and an hla class ii allele. to assess whether these hla linkages are specific for ragweed, we have investigated the association between hla antigens and the capacity of individuals to mount a specific ige response to melittin in ... | 1990 | 1974559 |
| randomized trial of postoperative patient-controlled analgesia vs intramuscular narcotics in frail elderly men. | postoperative use of as-needed intramuscular narcotics is potentially hazardous in frail elderly patients. patient-controlled analgesia (pca) allows patients to self-administer small boluses of narcotic, allowing better dose titration, enhanced responsiveness to variability in narcotic requirements, and reduction in serum narcotic level fluctuation. although theoretically useful, this method has not bee well studied in the elderly or medically ill. a prospective controlled trial among 83 higher- ... | 1990 | 1975490 |
| purification and pharmacological characterization of peptide toxins from the black mamba (dendroaspis polylepis) venom. | this paper reports the purification of 28 different peptides from the venom of the snake dendroaspis polylepis. these peptides represent 99% of the total peptide fraction in the venom. the 14 most cationic peptides form a structurally and functionally homogeneous group of analogs of the most abundant dendrotoxin toxin i (dtxi). they recognize antibodies raised against dtxi as well as brain membrane binding sites corresponding to k+ channels that are sensitive to dtxi and the bee venom peptide mc ... | 1990 | 1977212 |
| [nutritional care of severe acute head injury patients: formulas for early enteral alimentation]. | the objectives of this study were: 1) to determine a suitable enteral alimentation formula to meet calorie and protein requirements in acute severe head injury; and 2) to evaluate the effect of different alimentation formulas on the outcomes of 60 patients, who suffered from acutely severe head injury without other major injuries and who were treated without steroids. these patients were randomly divided into three groups and enteral feeding was started within 24 hrs. of admission or postoperati ... | 1990 | 1977869 |
| homeobox containing genes in the nematode caenorhabditis elegans. | we designed a unique 36-mer oligonucleotide probe, based on the most highly conserved amino acid sequences of antennapedia-like homeodomains and the codon bias of caenorhabditis elegans. this probe was then used to isolate four classes of genes from a c. elegans genomic library. sequencing reveals that we have isolated three new homeobox genes, designated ceh-1, ceh-9 and ceh-10. the fourth homeobox gene, ceh-11, has recently been described by schaller et al (nucleic acids res. 18, 2033-2036). t ... | 1990 | 1978282 |
| hemolytic potency and phospholipase activity of some bee and wasp venoms. | 1. the action of crude venoms of four aculeate species: apis mellifera, vespa crabro, vespula germanica and vespula vulgaris on human erythrocytes was investigated in order to determine the lytic and phospholipase activity of different aculeate venoms and their ability to induce red blood cell hemolysis. 2. bee venom was the only extract to completely lyse red blood cells at the concentration of 2-3 micrograms/ml. 3. phospholipase activity in all of the examined vespid venoms was similar and the ... | 1990 | 1981342 |
| electron probe microanalysis of calcium release and magnesium uptake by endoplasmic reticulum in bee photoreceptors. | honey bee photoreceptors contain large sacs of endoplasmic reticulum (er) that can be located unequivocally in freeze-dried cryosections. the elemental composition of the er was determined by electron probe x-ray microanalysis and was visualized in high-resolution x-ray maps. in the er of dark-adapted photoreceptors, the ca concentration was 47.5 +/- 1.1 mmol/kg (dry weight) (mean +/- sem). during a 3-sec nonsaturating light stimulus, approximately 50% of the ca content was released from the er. ... | 1991 | 1992466 |
| suicide-inhibitory bifunctionally linked substrates (siblinks) as phospholipase a2 inhibitors. mechanistic implications. | novel phospholipids that function as mechanism-based inhibitors for phospholipase a2 (pla2) are described. pla2-catalyzed hydrolysis of the sn-2 ester of these suicide-inhibitory bifunctionally linked substrates (siblinks) followed by a cyclization reaction generates a cyclic anhydride at the active site of the enzyme which leads to inhibition. structure/activity relationships for the siblinks substituents in the sn-1 and sn-2 position are delineated. time courses and efficiency of siblinks inhi ... | 1991 | 2002046 |
| evidence that a 16-kilodalton integral membrane protein antigen from schistosoma japonicum adult worms is a type a2 phospholipase. | type a2 phospholipase (pla2) activity has been observed in integral membrane protein extracts of schistosoma japonicum. antiserum raised against bee venom pla2 recognized a single 16-kda band in the parasite extracts; it also localized to antigen in the gut lining of fixed adult schistosomes as shown by immunofluorescence techniques. evidence was obtained that the molecule was expressed at low levels in comparison with other integral membrane proteins and was weakly immunogenic in rabbits. two o ... | 1991 | 2004822 |
| honeybee venom allergy: results of a sting challenge 1 year after stopping successful venom immunotherapy in 86 patients. | in 86 patients with a history of severe systemic reactions (srs) to honeybee stings confirmed by a positive skin test and/or rast to honeybee venom (hbv), immunotherapy (it) with hbv was stopped after 3 to 10 years. all patients had tolerated well a honeybee sting during it and were resubmitted to a sting 1 year after vit was stopped. at the latter time, 15 patients (17%) developed an sr and 71 did not. there was no difference between the two groups regarding age, sex, it duration, severity of t ... | 1991 | 2005323 |
| the role of retinal photoisomerase in the visual cycle of the honeybee. | the compound eye of the honeybee has previously been shown to contain a soluble retinal photoisomerase which, in vitro, is able to catalyze stereospecifically the photoconversion of all-trans retinal to 11-cis retinal. in this study we combine in vivo and in vitro techniques to demonstrate how the retinal photoisomerase is involved in the visual cycle, creating 11-cis retinal for the generation of visual pigment. honeybees have approximately 2.5 pmol/eye of retinal associated with visual pigment ... | 1991 | 2007885 |
| differences in primary structure among five phospholipases a2 from heloderma suspectum. | five increasingly anionic phospholipases a2 (pa1-pa5) exist in the venom of the lizard heloderma suspectum. we recently elucidated the sequence of pa5, the most abundant and most active variant, towards emulsified phosphatidylcholines. here we present the primary structures of pa2, pa3 (subvariants a and b) and pa4, based on edman degradation of tryptic, endoproteinase arg-c and chymotryptic fragments of the reduced and s-carboxymethylated proteins. pa1-pa5, considered collectively, belong to an ... | 1991 | 2013276 |
| binding and toxicity of apamin. characterization of the active site. | the structural features of apamin, a natural octadecapeptide from bee venom, enabling binding to its receptor and the expression of toxicity in mice, have been delineated by studying the effects on binding and toxicity of chemical modifications and amino acid substitutions in synthetic analogues. the results obtained indicate that the only hydrophobic residue, leucine at position 10, can be changed to alanine without a significant decrease in the specific activity. the need for a correct conform ... | 1988 | 2013287 |
| geographical overlap of two mitochondrial genomes in spanish honeybees (apis mellifera iberica). | restriction enzyme cleavage maps of mitochondrial dna from the spanish honeybee, apis mellifera iberica (hymenoptera: apidae), were compared with those from the european subspecies a. m. mellifera, a. m. ligustica, and a. m. carnica, and the african subspecies a. m. intermissa and a. m. scutellata. the mitochondrial dna (mtdna) of the two african subspecies can be distinguished by restriction fragment polymorphisms revealed by hinf i digests. two distinct mtdna types were found among spanish hon ... | 1991 | 2013694 |
| kinetics of melittin binding to phospholipid small unilamellar vesicles. | we have used the decrease in the fluorescence intensity of the single tryptophan residue of bee venom melittin at long emission wavelengths that accompanies binding of the peptide to phospholipid small unilamellar vesicles to determine the rate of binding through the use of stopped-flow fluorometry in the millisecond range. we have found the rate to depend on the degree of saturation of the lipid acyl chains as well as on the physical state of the bilayer, the net electric charge of the polar he ... | 1991 | 2015256 |
| contribution of proline-14 to the structure and actions of melittin. | the structure and dynamic properties of bee venom melittin and a synthetic analogue, [ala14]-melittin (melittin p14a), are compared, using high resolution 1h nuclear magnetic resonance (nmr) spectroscopy and amide exchange measurements in methanol. p14a is shown to adopt a regular, stable alpha-helical conformation in solution without the flexibility around the pro-14 residue found in melittin. p14a has twice the hemolytic activity of melittin but is less able to induce voltage-dependent ion con ... | 1991 | 2015901 |
| enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide. | the baculovirus/insect cell system has been remarkably successful in yielding high levels of synthesis of many proteins which have been difficult to synthesize in other host/vector systems. the system is also capable of secreting heterologous proteins, but with generally low efficiency. we have increased the efficiency of secretion of the system by using signal peptides of insect origin to direct the secretion of a foreign protein. the precursor of the plant cysteine protease papain (propapain) ... | 1991 | 2016060 |
| generation of a long-lived ige response in high and low responder strains of rat by co-administration of ricin and antigen. | certain strains of rats infested with the nematode parasite nippostrongulus brasiliensis mount vigorous, persistent immunoglobulin e (ige) responses. in the absence of parasites, adjuvants such as bordatella pertussis or al(oh)3 are needed to produce ige responses to soluble antigens. these are short-lived, even in high ige responder strains. in this study we have produced long-lived ige responses in both low (wistar) and high (brown norway) ige responder strains of rats by repeated injections o ... | 1991 | 2016124 |
| the role of microvascular free flaps in salvaging below-knee amputation stumps: a review of 22 cases. | twenty-two cases of traumatic below-knee amputation stumps with inadequate soft-tissue coverage salvaged with microvascular free flaps were reviewed retrospectively. all patients would have required an above-knee amputation for prosthesis fitting had microvascular free flaps not bee utilized. a total of 24 flaps were used in 22 patients; parascapular 11 (46%), foot filet six (25%), latissimus dorsi four (17%), lateral thigh, tensor fascia lata, and groin one (4%). free flaps were performed immed ... | 1991 | 2020035 |
| crystal structure of bee-venom phospholipase a2: correction. | 1991 | 2028251 | |
| evaluation of severe reactions to sweat bee stings. | thirteen patients with severe reactions to sweat bee stings were evaluated. eleven patients had had systemic reactions and two, large local reactions. skin testing and rast with venom of other hymenoptera and whole body extract of sweat bees disclosed negative reactions to both skin tests and rast of all other insects tested in eight, while three showed sensitivity to honey bee. only one patient had a positive rast reaction to whole body extract of sweat bee. whole body extract of sweat bee caus ... | 1991 | 2035902 |
| apamin, a highly potent blocker of the ttx- and mn2(+)-insensitive fast transient na+ current in young embryonic heart. | the whole-cell current clamp and voltage clamp techniques were used to record the slow na+ action potentials (aps) and the inward current in cultured single ventricular cells isolated from young (3 day-old) embryonic chicks. the slow na+ aps had a +vmax of 21.5 +/- 7.5 v/s (in 10 different single cells) and the macroscopic inward current responsible for the rising phase of these aps was a fast transient (ft) current. the ft inward current was sensitive to changes in [na]o but not to changes in [ ... | 1991 | 2038068 |
| [when can desensitization with bee and wasp venom be stopped?]. | 1991 | 2038399 | |
| energy and respiratory metabolism in cystic fibrosis: the influence of carbohydrate content of nutritional supplements. | the basal energy expenditure (bee) in a group of adolescent and young adult patients with cystic fibrosis (cf) with mild lung disease was 97 +/- 6% of that predicted by the harris-benedict equation (which estimates bee by age, sex, height, and weight). the bee of a group with more severe lung disease was 117 +/- 5% of that predicted by the harris-benedict equation, due primarily to a 14% greater oxygen consumption (vo2) and 24% greater co2 production (vco2) compared with milder lung disease (p l ... | 1991 | 2051272 |
| the function of allergy: immunological defense against toxins. | this paper proposes that the mammalian immune response known as "allergy" evolved as a last line of defense against the extensive array of toxic substances that exist in the environment in the form of secondary plant compounds and venoms. whereas nonimmunological defenses typically can target only classes of toxins, the immune system is uniquely capable of the fine-tuning required to target selectively the specific molecular configurations of individual toxins. toxic substances are commonly alle ... | 1991 | 2052671 |
| snake venom cardiotoxins and bee venom melittin activate phospholipase c activity in primary cultures of skeletal muscle. | the effects of cardiotoxin fractions from naja naja kaouthia and naja naja atra snake venoms and synthetic melittin peptide were examined on lipolytic activity in red blood cells and primary skeletal muscle cultures. both native cardiotoxin fractions caused considerable production of free fatty acids in red blood cells. this production was abolished when the fractions were first treated with p-bromophenacyl bromide to reduce the venom phospholipase a2 activity contamination. in equine and human ... | 1991 | 2054159 |
| association of exogenous dna with cattle and insect spermatozoa in vitro. | spermatozoa isolated from domestic cattle (bos taurus), the australian sheep blowfly (lucilia cuprina), and the honeybee (apis mellifera) are capable of binding exogenous radiolabeled linear dna. both motile and nonmotile bovine sperm exhibit four distinct patterns of dna association. following treatment with dnase i, the relative proportion of one of these patterns increases specifically in living sperm, suggesting that a small proportion of dna that associates with bovine sperm may be sequeste ... | 1991 | 2054177 |
| human fatalities caused by wasp and bee stings in sweden. | bee and wasp stings represent an uncommon cause of death. in a retrospective study of all fatalities due to venomous animals in sweden during a 10-year-period, we found 19 fatalities due to wasps, 1 due to bees but none due to snake bite. this gives an annual incidence of 0.2 per million inhabitants. most of the deceased were older than 50 years and had an underlying cardiovascular disease while previous severe reactions after insect stings were uncommon. the number of stings was not a factor of ... | 1991 | 2054310 |
| calcium-regulated interactions of human alpha-lactalbumin with bee venom melittin. | affinity chromatography, fluorescence and circular dichroism spectroscopy methods have been used to study the interaction of melittin, a 26-residue peptide from bee venom, with ca2(+)-binding alpha-lactalbumin from human milk. it has been revealed that melittin binds to the apo- and acidic states of alpha-lactalbumin while the presence of ca2+ makes the interaction essentially weaker. the association constant for the complex of melittin with apo-alpha-lactalbumin determined from spectropolarimet ... | 1991 | 2059662 |
| [insect venom allergy in childhood]. | hymenoptera venom allergy is relatively rare in childhood. in 59 children, aged 2-16 years, with a systemic allergic reaction we could detect the following findings: there was no difference between males and females. relatives of these children had more atopic diseases than normals. the degree of clinical manifestation was not so pronounced as in adults. rast was more sensitive in detecting bee venom allergy whereas intradermal test showed a higher sensitive in wasp venom allergy. the diagnostic ... | 1991 | 2063728 |
| range perception through apparent image speed in freely flying honeybees. | when negotiating a narrow gap, honeybees tend to fly through the middle of the gap, balancing the distances to the boundary on either side. to investigate the basis of this "centering response," bees were trained to fly through a tunnel on their way to a feeding site and back, while their flight trajectories were filmed from above. the wall on either side carried a visual pattern. when the patterns were stationary vertical gratings, bees tended to fly through the middle of the tunnel, i.e. along ... | 1991 | 2069903 |
| quantitative autoradiographic localization of [125i]alpha-bungarotoxin binding sites in the honeybee brain. | binding sites for the nicotinic acetylcholine receptor antagonist, [125i]alpha-bungarotoxin, were localized in the honeybee brain by in vitro autoradiography. highest binding site densities were localized in the suboesophageal ganglion, the optic tubercles, optic lobes medulla and lobula, antennal lobes, dorsal lobes and the alpha-lobes of the mushroom bodies. the distribution pattern of these putative nicotinic acetylcholine receptors suggests that acetylcholine is involved in several sensory p ... | 1990 | 2073597 |
| glucagon as a non species-specific regulator of the glycaemia in honeybee (apis mellifica). | it is known that hormones which exist in vertebrates are very old in respect to evolution. the presence of insulin and glucagon in insects had already been suggested in the early sixties. in this study honeybees are living in an "artificial hive" in the german laboratory under exactly standardized conditions. in the french laboratory standardized conditions yielded because only emerging worker bees were used i.e. those which were just slipping from the honeycomb alveoli. it seemed of interest to ... | 2006 | 2073968 |
| rapid activation of the non-toxic basic isoform of phospholipase a2 from naja mossambica mossambica (spitting cobra) by long-chain fatty acylation. | purified phospholipase a2 from naja naja (common indian cobra) venom, a basic non-toxic isoform and a toxic isoform from naja mossambica mossambica venom were treated with an equimolar amount of oleoyl imidazolide, a known activator of bee venom phospholipase a2. the ability of the first two enzymes to induce erythrocyte lysis was strongly activated while hydrolysis of dioctanoyl phosphatidyl choline was only weakly activated. the toxic enzyme showed little change in either assay. the susceptibl ... | 1990 | 2080519 |
| projectin is an invertebrate connectin (titin): isolation from crayfish claw muscle and localization in crayfish claw muscle and insect flight muscle. | a filamentous protein was isolated from crayfish claw muscle. this protein had physiochemical properties very similar to vertebrate skeletal muscle connectin (titin), although its apparent molecular mass (approximately 1200 kda) was considerably lower than that of connectin (approximately 3000 kda). polyclonal as well as monoclonal antibodies against chicken skeletal muscle connectin reacted with the 1200 kda protein from crayfish claw muscle. conversely, polyclonal antibodies against crayfish 1 ... | 1990 | 2084147 |
| [morphological observation of effect of bee pollen on intercellura lipofuscin in nih mice]. | by histochemical method we have observed the effect of bee pollen on lipofuscin in nih mice. the results show that oral administration of bee pollen at 10 g/kg/d markedly reduces lipofuscin in cardiac muscle in aged mice, significantly inhibits the increase of lipofuscin in cardiac muscle, liver, brain and adrenal gland cells induced by orally given peroxidized corn oil or iv alloxan. this action may be related with the anti-ageing effect of bee pollen. | 1990 | 2092719 |
| laser confocal scanning microscopy of the surface membrane/t-tubular system and the sarcoplasmic reticulum in insect striated muscle stained with dilc18(3). | the structure of the surface membrane/transverse tubular (t-tubular) system and of the sarcoplasmic reticulum (sr) of the labial adductor muscle of the honey bee (apis mellifera) was examined by laser confocal scanning microscopy, after staining with the fluorescent membrane probe diic18(3). the following components of the surface membrane/t-tubular system were visualized: transverse tubular networks that are located in the a-band close to the a-i junction and form dyads with the sr, longitudina ... | 2007 | 2100146 |
| comparison of low, medium, and high carbohydrate formulas for nighttime enteral feedings in cystic fibrosis patients. | this study examined whether the increase in co2 production (vco2) and ventilatory demands by carbohydrate loading with different formulas during nighttime enteral feedings could be detrimental in young adult cystic fibrosis patients with moderate to advanced lung disease. ten patients age 17 to 24 (mean 21.4 years) received 1000 kcal/m2 of a low (pulmocare), medium (ensure plus), and high (vivonex hn) carbohydrate formula in random order. eight patients had severe, and two moderate obstructive p ... | 2017 | 2109114 |
| antibiotic activity of natural products: 1. propolis. | material extracted from propolis (bee glue) by alkaline aqueous solvents or organic solvents showed weak inhibitory activity in vitro against certain species of gram-positive bacteria. no antimicrobial activity was detected in urine from three volunteers who had taken 500 mg propolis three times a day for 3 days. | 1990 | 2110610 |
| apis mellifera cytoplasmic elongation factor 1 alpha (ef-1 alpha) is closely related to drosophila melanogaster ef-1 alpha. | using low stringency hybridisation with a drosophila melanogaster ef-1 alpha gene fragment we have isolated a genomic dna clone encoding elongation factor 1 alpha (ef-1 alpha) from apis mellifera. the hybridising apis mellifera sequence could be delineated to two small ecori fragments that were also revealed by genomic southern hybridisation. by comparison with the corresponding drosophila melanogaster data the complete translational reading frame has been deduced. it is interrupted by two inter ... | 1990 | 2116322 |
| esterase isozymes in a solitary bee, megachile rotundata (fab.): characterization, developmental multiplicity, and adult variability. | this study describes the biochemical characterization and genetic variation of cytosolic esterases in the alfalfa leafcutting bee, megachile rotundata (fab.). esterase isozymes were separated by nondenaturing polyacrylamide gel electrophoresis and isoelectric focusing and characterized by inhibition with eserine sulfate, edta, paraoxon, and p-hydroxymercuribenzoate. based on inhibition patterns and substrate specificity, there are major differences between adults and immature forms and more subt ... | 1990 | 2125203 |
| electron microscopic structure of the cephalic gland of the bee andrena variabilis smith (hymenoptera: apoidea). | the structure of the glandular tissue located beneath the eye sulcus in andrena variabilis smith was studied by means of light and electron microscopy. the glandular tissue was found to be constituted of single-layered, high cylindrical epithelial cells. electron microscopy revealed strong foldings of the cell membrane on the basal pole of the gland cells. next to the basal lamina, nerve fibres were found with great frequency. the central, perinuclear zone of the gland cells was rich in cell org ... | 2007 | 2131710 |
| purification and characterization of honey bee vitellogenin. | a protocol has been developed for the purification of vitellogenin from the honey bee, apis mellifera. purification allows for the first characterization of a vitellogenin from the large order hymenoptera. hymenopteran vitellogenins are unusual among insect vitellogenins in that they contain only one type of apoprotein. the honey bee vitellogenin was isolated from hemolymph of honey bee queens by a combination of density gradient ultracentrifugation, ion-exchange chromatography, and affinity chr ... | 1990 | 2134180 |
| can total parenteral nutrition reverse hypoalbuminemia in oncology patients? | to determine if hypoalbuminemia is a consequence of protein-energy malnutrition rather than an associated feature of cancer, we reviewed results when vigorous nutritional support was given to 13 men and 9 women who had solid tumors (head and neck [10], gastrointestinal [8], other [4]) and an initial serum albumin less than 3.5 g/dl. patients had received a minimum of 21 days of nutritional support with total parenteral nutrition (tpn) supplying an average daily energy intake of at least 1.5 x ba ... | 2004 | 2136002 |
| a method to generate antigen-specific suppressor t cells in vitro from peripheral blood t cells of honey bee venom-sensitive, allergic patients. | peripheral blood mononuclear cells of patients allergic to honey bee venom were stimulated with denatured bee venom phospholipase a2, and the antigen-activated t cells were propagated for 4 days by human il-2 in the presence or absence of recombinant human lipocortin i. upon antigenic stimulation with the denatured phospholipase a2 and autologous monocytes or by cross-linking of cd3 by anti-cd3 antibody, the activated t cells, which had propagated by il-2 alone, formed n-glycosylated ige-binding ... | 1990 | 2138201 |
| rhodopsin reconstitution in bleached rod outer segment membranes in the presence of a retinal-binding protein from the honeybee. | the physiological role of a retinal-binding protein from honeybee is investigated. this protein, upon previous loading with all-trans retinal and subsequent irradiation with monochromatic light of wavelength 490 nm, is able to promote the reconstitution of rhodopsin when added to a suspension of opsin membranes from bleached bovine rod outer segments. in this respect this retinal-binding protein could have a role very similar to that postulated for the well-known cephalopod retinochrome, that se ... | 1990 | 2143484 |
| acute renal failure due to multiple bee stings--case reports. | this report deals with a father and his son who developed acute renal failure following multiple bee stings. the renal lesion in these patients appears to be due to rhabdomyolysis caused by the bee venom. the other mechanisms are also discussed. the need for clinicians to be aware of acute renal failure as a complication of bee stings is stressed. | 1990 | 2152058 |
| phospholipases: old enzymes with new meaning. | phospholipases are enzymes that hydrolyze specific portions of phospholipid molecules. their role in the digestion of exogenous phospholipids and as the active principle in snake and bee venoms has long been appreciated. interest has increased in phospholipases recently because of new data implicating them in the inflammatory response. the ability of phospholipases to hydrolyze bacterial phospholipids has also received considerable attention. these new data have brought pertinence to studies of ... | 1990 | 2157620 |
| [125i]azidosalicylyl melittin binding domains: evidence for a polypeptide receptor on the gastric (h+ + k+)atpase. | we have previously shown that melittin, a bee venom peptide, potently inhibited the catalytic and transport functions of rabbit gastric (h+ + k+)atpase. a radioactive photoaffinity analog of melittin, ([125i]azidosalicylyl melittin), labeled the (h+ + k+)atpase. these results suggested that melittin exerted inhibitory effects through direct interaction with the (h+ + k+)atpase. in this study we attempt to define the melittin-binding domain of the (h+ + k+)atpase using conformation-dependent prot ... | 1990 | 2158280 |