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patterns of variation in the seasonal dynamics of listronotus maculicollis (coleoptera: curculionidae) populations on golf course turf.listronotus maculicollis (kirby) is a burgeoning pest of poa annua l. in the northeast united states. to develop an understanding of its spatial and temporal association with golf course landscapes, we described the patterns of variation in certain population parameters across site (fayetteville and ithaca, ny), year (2004-2006), and management habitat (fairway, intermediaterough, rough). in weekly surveys along fairway transects, we sampled larvae (identified to instar) by soil core extraction ...200819161687
a comparison of bark and ambrosia beetle communities in two forest types in northern thailand (coleoptera: curculionidae: scolytinae and platypodinae).many ecological studies of tropical insects are based on small sample sizes or lack sampling scheme rigor, which prevents testing ecological hypotheses and comparing samples from different sites and times. here we present the results of quantitative trapping of bark and ambrosia beetles over 2 yr at two localities in northern thailand separated by 5 km, 1,100 m in altitude, and in different forest types. beetles were collected using a spatially and temporally standardized sampling scheme, follow ...200819161689
effect of host plants on successful parasitism by haeckeliania sperata (hymenoptera: trichogrammatidae) on diaprepes abbreviatus (coleoptera: curculionidae) eggs.diaprepes abbreviatus l. is a highly polyphagous root weevil that causes damage to several fruit crops, ornamental plants, and other naturally occurring plants in florida. haeckeliania sperata pinto is a gregarious endoparasitoid that attacks d. abbreviatus eggs. we hypothesized that the reproductive success of h. sperata is affected by the host plant of d. abbreviatus. six host plants with varying degrees of pubescence were used to determine the effect of leaf trichomes on the searching behavio ...200819161701
specificity and redundancy in the olfactory system of the bark beetle ips typographus: single-cell responses to ecologically relevant odors.we screened 150 olfactory sensilla in single-sensillum recordings to unravel the mechanisms underlying host selection in the spruce bark beetle, ips typographus (coleoptera: curculionidae: scolytinae). odor stimuli comprised of pheromone (various bark beetle spp.), host, and non-host compounds elicited strong and selective responses from 106 olfactory receptor neurons (orns), 45 of which were tuned to pheromone compounds, 37 to host compounds, and 24 to non-host volatiles (nhv). in addition, 26 ...200919233334
comparisons of boll weevil (coleoptera: curculionidae) pheromone traps with and without kill strips.boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), eradication programs typically equip pheromone traps with an insecticide-impregnated kill strip. these strips are intended to kill captured insects, thereby simplifying trap servicing and reducing the loss of weevils from predation and escape. however, the effectiveness of kill strips has not been extensively evaluated. we examined the influences of kill strips on weevil captures, trap servicing, and the incidences of w ...200919253635
artificial substrates for oviposition and larval development of the pepper weevil (coleoptera: curculionidae).the pepper weevil, anthonomus eugenii cano (coleoptera: curculionidae), is a major pest of cultivated peppers (capsicum spp.) and other cultivated and wild species within the family solanaceae. laboratory study of this insect, as well as its biological control agents, will be greatly facilitated by an artificial rearing system that does not rely on pepper fruit. an egg collection method and amendments to a standard larval diet were investigated for use in the rearing of this weevil. spherical sa ...200919253644
billbug (coleoptera: curculionidae) species composition, abundance, seasonal activity, and developmental time in florida.billbugs (coleoptera: curculionidae: sphenophorus spp.) are common pests whose damage is often misdiagnosed on turfgrass in the united states. consequently, florida turfgrass managers have been struggling to satisfactorily control billbug outbreaks. thus, we sought to determine the species complex, abundance, seasonality, and fecundity of key sphenophorus spp. from field collections, and quantify duration of developmental and daily activity periods through greenhouse rearing tests. from january ...200919253650
pyrethroid resistance in populations of listronotus maculicollis (coleoptera: curculionidae) from southern new england golf courses.topical application bioassays of bifenthrin and lambda-cyhalothrin were conducted on field-collected populations of listronotus maculicollis kirby (coleoptera: curculionidae) from eight southern new england golf courses, six in connecticut, one in massachusetts, and one in rhode island. concentrations versus mortality regression lines were estimated to compare the ld50 values and resistance ratios for each insecticide and each population. the ld50 ranges for bifenthrin and lambda-cyhalothrin wer ...200919253659
from augmentation to conservation of entomopathogenic nematodes: trophic cascades, habitat manipulation and enhanced biological control of diaprepes abbreviatus root weevils in florida citrus groves.the use of entomopathogenic nematodes (epn) for management of the root weevil, diaprepes abbreviatus, in florida citrus groves is considered a biological control success story and typically involves augmentation in which epn are applied inundatively as biopesticides to quickly kill the pest. however, recent evidence indicates that efficacy of epn applications in florida citrus depends on soil type. they are very effective in the well drained coarse sands of the central ridge but often less so in ...200819259523
recycling potential and fitness of steinernematid nematodes cultured in curculio caryae and galleria mellonella.the recycling potential of entomopathogenic nematodes in the pecan weevil, curculio caryae, following inundative applications is an important factor in considering whether nematodes could be incorporated into a c. caryae management strategy. our objective was to determine the recycling potential and fitness of steinernema carpocapsae and s. riobrave cultured in c. caryae. to estimate fitness and quality, we reared nematodes in larvae of c. caryae and in the commonly used standard host, the great ...200519262838
steinernema diaprepesi n. sp. (rhabditida: steinernematidae), a parasite of the citrus root weevil diaprepes abbreviatus (l) (coleoptera: curculionidae).a nematode collected from diaprepes abbreviatus is identified and described as a new species, steinernema diaprepesi n. sp. the new species is closely related to s. feltiae, s. glaseri, and s. oregonense and can be distinguished from these species by the following characteristics: males: spicule averaging 79 (71-90) microm and spicule shape; d% (distance from anterior end to excretory pore/ esophagus length x 100) about 80; the ratio sw (spicule length/anal body width) about 1.8. females: vulva ...200219265926
incidence of endemic entomopathogenic nematodes following application of steinernema riobrave for control of diaprepes abbreviatus.control of diaprepes abbreviatus by endemic and exotic entomopathogenic nematodes (epn) was monitored during 2000-2001 in two citrus orchards in central florida (bartow and poinciana). caged sentinel insect larvae were buried beneath citrus trees for 7 days at 1 to 2-month intervals from april to october each year. at bartow, the survey occurred in experimental plots that were (i) not treated with commercial epn, (ii) treated twice annually since 1998 with commercially formulated steinernema rio ...200319265992
effects of culture method and formulation on the virulence of steinernema riobrave (rhabditida: steinernematidae) to diaprepes abbreviatus (coleoptera: curculionidae).the diaprepes root weevil, diaprepes abbreviatus, is a pest of vegetables, ornamental plants, sugarcane, and citrus in florida and the caribbean. the entomopathogenic nematode, steinernema riobrave, can reduce larval populations of d. abbreviatus substantially. efficacy of entomopathogenic nematodes, however, may be affected by culture method and formulation. using d. abbreviatus as the host, we compared the efficacy of two commercial s. riobrave formulations, a liquid and a waterdispersible gra ...200019270978
biological control of the pecan weevil, curculio caryae (coleoptera: curculionidae), with entomopathogenic nematodes.steinernema carpocapsae (weiser) strain a11, s. feltiae (filipjev) strain sn, and heterorhabditis bacteriophora poinar strains hp88 and georgia were tested for their efficacy as biological control agents of the pecan weevil, curculio caryae (horn), in pecan orchard soil-profile containers under greenhouse conditions. percentage c. caryae parasitism by s. carpocapsae and h. bacteriophora strain hp88 and georgia was consistently poor when applied either prior to or following c. caryae entry into t ...199319279746
association of the red ring nematode and other nematode species with the palm weevil, rhynchophorus palmarum.the palm weevil, rhynchophorus palmarum (l.), was collected in cocoons from red ring-diseased coconut palms (cocos nucifera l.) in trinidad and tobago. juveniles of five species of nematodes were extracted from the genitalia and macerated bodies of newly emerged adults of the palm weevil: rhadinaphelenchus cocophilus (cobb) goodey (the red ring nematode), teratorhabditis sp., diplogasteritus sp., mononchoides sp., and bursaphelenchus sp. over 90% of newly emerged weevil females and males were in ...199019287703
teratorhabditis palmarum n. sp. (nemata: rhabditidae): an associate of rhynchophorus palmarum and r. cruentatus.teratorhabditis palmarum n. sp., an associate of the palm weevils rhynchophorus palmarum and r. cruentatus is described. teratorhabditis palmarum was isolated from newly emerged adults and cocoons of r. palmarum from red-ring diseased coconut palms, cocos nucifera, in trinidad and ecuador, and from red-ring diseased oil palms, elaeis guineensis, in colombia. teratorhabditis palmarum was also associated internally with newly emerged adults of r. cruentatus from mature transplanted cabbage palmett ...199019287730
nemtaode-vector relationships in the pine wilt disease system.pinewood nematode, bursaphelenchus xylophilus, is the causal agent of pine wilt disease in north america and japan. dispersal stage dauer larvae are transported to new host trees on the body surface and within the tracheal system of several beetle species. worldwide, 21 species of cerambycidae, 1 genus of buprestidae, and 2 species of curculionidae are known to carry pinewood nematode dauer larvae upon emerging from nematode-infested trees. five species of cerambycids in the genus monochamus are ...198819290206
first in line or first in time? effects of settlement order and arrival date on reproduction in a group-living beetle dendroctonus ponderosae.1. in group-living organisms, individuals that initiate aggregations, termed pioneers, may suffer higher mortality costs than individuals that join established aggregations. here we examine the hypothesis that aggregation initiators achieve higher reproductive success in the early phases of colonization, potentially through lower competition and increased access to the resource (finder's advantage), and that this benefit is sufficient to outweigh the costs of pioneering. 2. we also examine the r ...200919292705
susceptibility of the carrot weevil (coleoptera: curculionidae) to steinernema feltiae, s. bibionis, and heterorhabditis heliothidis.larvae, pupae, and adults of the carrot weevil (listronotus oregonensis) were infected and killed by the three entomophagous nematodes (steinernema feltiae, s. bibionis, and heterorhabditis heliothidis) under controlled conditions. third-stage larvae were more susceptible than pupae or adults. s. feltiae and s. bibionis were the most aggressive nematode species, causing larval mortality after 24-48 hours in both continuous and 2-hour contact with nematode suspension. the nematodes multiplied suf ...198519294108
flight take-off and walking behavior of insecticide-susceptible and - resistant strains of sitophilus zeamais exposed to deltamethrin.insects have evolved a variety of physiological and behavioral responses to various toxins in natural and managed ecosystems. however, insect behavior is seldom considered in insecticide studies although insects are capable of changing their behavior in response to their sensory perception of insecticides, which may compromise insecticide efficacy. this is particularly serious for insect pests that are physiologically resistant to insecticides since insecticide avoidance may further compromise t ...200919302721
ambrosiella beaveri, sp. nov., associated with an exotic ambrosia beetle, xylosandrus mutilatus (coleoptera: curculionidae, scolytinae), in mississippi, usa.xylosandrus mutilatus is an asian ambrosia beetle that has recently established in mississippi, texas, alabama, and possibly florida, usa. we investigated the fungi associated with the mycangia (specialized fungus-transporting structures) of x. mutilatus in mississippi. mycangia consistently yielded an ambrosiella sp. which was subsequently found to be closely related to, but distinct from, other ambrosiella species affiliated with ceratocystis. this ambrosiella is described herein as ambrosiell ...200919319658
a comparison of alternative plant mixes for conservation bio-control by native beneficial arthropods in vegetable cropping systems in queensland australia.cucurbit crops host a range of serious sap-sucking insect pests, including silverleaf whitefly (slw) and aphids, which potentially represent considerable risk to the australian horticulture industry. these pests are extremely polyphagous with a wide host range. chemical control is made difficult due to resistance and pollution, and other side-effects are associated with insecticide use. consequently, there is much interest in maximising the role of biological control in the management of these s ...201019323854
praecocilenchus rhaphidophorus n. gen., n. sp. (nematoda: aphelenchoidea) parasitizing rhynchophorus bilineatus (montrouzier) (coleoptera: curculionidae) in new britain.praecocilenchus rhaphidophorus n. gen., n. sp. is described as a new endoparasitic aphelenchoid nematode parasitizing adults of the palm weevil, rhynchophorus bilineatus (montrouzier). p. rhaphidophorus is unusual in that juveniles develop to maturity within the female uterus and thin, needle-shaped crystals form in the intestines of mature parasitic females. hundreds of parasitic female nematodes were found in the body cavity of infected hosts. the role of this parasite as a biological control ...196919325682
temporal lags and overlap in the diversification of weevils and flowering plants.the extraordinary diversity of herbivorous beetles is usually attributed to coevolution with angiosperms. however, the degree and nature of contemporaneity in beetle and angiosperm diversification remain unclear. here we present a large-scale molecular phylogeny for weevils (herbivorous beetles in the superfamily curculionoidea), one of the most diverse lineages of insects, based on approximately 8 kilobases of dna sequence data from a worldwide sample including all families and subfamilies. est ...200919365072
host use and crop impacts of oribius marshall species (coleoptera: curculionidae) in eastern highlands province, papua new guinea.oribius species are small flightless weevils endemic to the island of new guinea and far northern cape york, australia. the adults feed externally on leaves, developing fruit and green bark, but their impact as pests and general host use patterns are poorly known. working in eastern highlands province, papua new guinea, we carried out structured host use surveys, farmer surveys, shade-house growth trials and on-farm and on-station impact trials to: (i) estimate the host range of the local oribiu ...201019366475
effect of weather on ips typographus (coleoptera curculionidae) phenology, voltinism, and associated spruce mortality in the southeastern alps.summer drought associated with high temperatures recorded in the last few years has given rise to outbreaks of bark beetles developing in weakened host trees. the aim of this study was to investigate the possible weather effect on the biology of and damage caused by ips typographus l. in the southeastern alps. the study was carried out recording temperature (1962-2007), precipitation (1922-2007), and the damage caused by i. typographus (1993-2007). in addition, data from pheromone-baited traps ( ...200919389278
woody stem galls interact with foliage to affect community associations.gall wasps (hymenoptera: cynipidae) hijack the physiology of their host plant to produce galls that house wasps throughout their immature stages. the gall-maker-host plant interaction is highly evolved, and galls represent an extended phenotype of the gall wasp. we evaluated two-way interactions between stem galls produced by dryocosmus kuriphilus yasumatsu on castanea spp. (fagales: fagaceae) and foliage directly attached to galls (gall leaves) using gall leaf excision experiments and herbivore ...200919389291
electrophysiological and behavioral responses of dendroctonus valens (coleoptera: curculionidae: scolytinae) to four bark beetle pheromones.the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), has caused extensive mortality of pinus tabuliformis carrière in north central china. the electrophysiological and behavioral activities of the four bark beetle pheromones, frontalin, exo-brevicomin, trans-verbenol, and cis-verbenol, singly or in combination with host-produced kairomones, were tested on red turpentine beetles. both sexes showed a consistent electrophysiological response to the four te ...200919389297
repellent activity of alligator pepper, aframomum melegueta, and ginger, zingiber officinale, against the maize weevil, sitophilus zeamais.the repellent activity of alligator pepper, aframomum melegueta, and ginger, zingiber officinale (zingiberaceae), against the maize weevil, sitophilus zeamais (coleoptera: curculionidae), was investigated in four-way olfactometer bioassays. results showed that vacuum distilled a. melegueta and z. officinale extracts were repellent towards adult s. zeamais both in the absence and the presence of maize, zea mays, grains. bioassay-guided liquid chromatographic fractionation of the distillates showe ...200919394981
a folded and functional synthetic pa1b: an interlocked entomotoxic miniprotein.pa1b (pea albumin 1, subunit b) is a hydrophobic, 37-amino acid miniprotein isolated from pea seeds (pivum sativum), crosslinked by three interlocked disulfide bridges, signature of the ick (inhibitory cystine-knot) family. it acts as an entomotoxic factor against major insect pests in stored crops and vegetables, making it a promising bioinsecticide. here we report an efficient and simple protocol for the production of large quantities of highly pure, biologically active synthetic pa1b. the fea ...200919399851
rnai in the cereal weevil sitophilus spp: systemic gene knockdown in the bacteriome tissue.the weevils sitophilus spp. are among the most important cosmopolitan pests of stored cereal grains. however, their biology and physiology are poorly understood, mainly because the insect developmental stages take place within cereal grains and because of the lack of gene specific molecular manipulation.200919445662
phylogeographical patterns of a generalist acorn weevil: insight into the biogeographical history of broadleaved deciduous and evergreen forests.climatic changes during glacial periods have had a major influence on the recent evolutionary history of living organisms, even in temperate forests on islands, where the land was not covered with ice sheets. we investigated the phylogeographical patterns of the weevil curculio sikkimensis (curculionidae), a generalist seed predator of fagaceae plants living in both deciduous oak and evergreen forests of japan. its genetic structure was compared to that of another host-specific seed predator, c. ...200919445688
molecular diagnostic for boll weevil (coleoptera: curculionidae) based on amplification of three species-specific microsatellites.the boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), is a serious pest of cultivated cotton, gossypium hirsutum l., in the americas, and reinfestation of zones from which they have been eradicated is of perpetual concern. extensive arrays of pheromone traps monitor for reintroductions, but occasionally the traps collect nontarget weevils that can be misidentified by scouts. for example, the congeneric pepper weevil, anthonomus eugenii cano, and other superficially sim ...200919449658
scelianoma elydimorpha, a new genus and new species of entimine weevil from southwestern puerto rico (coleoptera: curculionidae, entiminae).scelianoma elydimorpha franz & girón, new genus and new species, an entimine weevil endemic to southwestern puerto rico, is described and illustrated in detail, and assigned to the tribe eustylini (curculionidae: entiminae). scelianoma is distinguished from related taxa by the following diagnostic features: shape narrowly elongate, length/width ratio 2.8-3.5; integument with densely arranged scales; pronotum without conspicuous sculpturing; male metatibia with row of 5-7 teeth, each tooth with a ...200919488511
[parisoschoenus obesulus casey (coleoptera: curculionidae) is not a pest of young coconut tree fruits].this study aimed to evaluate if parisoschoenus obesulus casey only attacks naturally aborting coconuts and, consequently, is not a pest of young fruits of coconut tree. aiming to test this hypothesis, inflorescences at diverse stages of physiological development were offered to individuals of p. obesulus. the results showed that only aborting fruits were colonized by p. obesulus corroborating the established hypothesis.200919488515
effect of geographic isolation on genetic differentiation in dendroctonus pseudotsugae (coleoptera: curculionidae).genetic structure of phytophagous insects has been widely studied, however, relative influence of the effect of geographic isolation, the host plant or both has been subject of considerable debate. several studies carried out on bark beetles in the genus dendroctonus evaluated these factors; nonetheless, recent evidence has shown that genetic structuring is a more complex process. our goal was to examine the effect of geographic isolation on genetic structure of the douglas-fir beetle dendrocton ...200919490169
effects of gallery density and species ratio on the fitness and fecundity of two sympatric bark beetles (coleoptera: curculionidae).interspecific interactions among tree-killing bark beetle species may have ecologically important consequences on beetle population dynamics. using two tree-killing beetle species (dendroctonus brevicomis and d. frontalis), we performed observational and experimental studies to verify cross-attraction and co-colonization under field conditions in northern arizona and test the effects of gallery density and species ratio on response variables of average gallery length, offspring size (progeny fit ...200919508772
occurrence of trichosirocalus horridus (coleoptera: curculionidae) on native cirsium altissimum versus exotic c. vulgare in north american tallgrass prairie.postrelease studies can provide data with which to evaluate expectations based on prerelease tests of biological control insects. in 2004, we observed trichosirocalus horridus panzer (coleoptera: curculionidae), the rosette weevil introduced into north america against eurasian thistles, feeding on native tall thistle, cirsium altissimum l. spreng., in tallgrass prairie. in this study, we examined the rosette weevil's use of tall thistle, compared with its use of the co-occurring exotic bull this ...200919508782
volatiles released from vaccinium corymbosum were attractive to aegorhinus superciliosus (coleoptera: curculionidae) in an olfactometric bioassay.the objective of this study was to evaluate the role of host volatiles in the relationship between a blueberry plant vaccinium corymbosum l. and the raspberry weevil aegorhinus superciliosus (guérin) (coleoptera: curculionidae), the principal pest of blueberry in the south of chile. volatiles from the aerial part of different phenological stages of the host were collected on porapak q and analyzed by coupled gas chromatography-mass spectrometry (gc-ms). several chemical groups were identified in ...200919508787
response of cranberry weevil (coleoptera: curculionidae) to host plant volatiles.the oligophagous cranberry weevil, anthonomus musculus say, causes economic losses to blueberry growers in new jersey because females deposit eggs into developing flower buds and subsequent larval feeding damages buds, which fail to produce fruit. a cost-effective and reliable method is needed for monitoring this pest to correctly time insecticide applications. we studied the behavioral and antennal responses of adult a. musculus to its host plant volatiles to determine their potential for monit ...200919508797
electroantennogram technique for conotrachelus nenuphar (coleoptera: curculionidae).reliable electroantennogram (eag) responses were obtained from the plum curculio, conotrachelus nenuphar (herbst), by using a whole body mount with glass electrodes filled with kcl-saturated agcl solution that penetrated the antennal club (recording electrode) and the membrane attached to the cervical sclerite between the thorax and the head (indifferent electrode). known attractive odor sources including extracts of headspace collections of 'stanley' plum and 'empire' apple tissues and a synthe ...200919508798
bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae).the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ...200919543937
acorn crop size and pre-dispersal predation determine inter-specific differences in the recruitment of co-occurring oaks.the contribution of pre-dispersal seed predation to inter-specific differences in recruitment remains elusive. in species with no resistance mechanisms, differences in pre-dispersal predation may arise from differences in seed abundance (plant satiation) or in the ability of seeds to survive insect infestation (seed satiation). this study aimed to analyse the impact of pre-dispersal acorn predation by weevils in two co-occurring mediterranean oaks (quercus ilex and quercus humilis) and to compar ...200919544074
evaluation of natural diatomaceous earth deposits from south-eastern europe for stored-grain protection: the effect of particle size.the use of diatomaceous earths (des) provides a promising alternative to the use of contact insecticides in stored-product ipm. geographical origin and the physical properties of a given de may affect its insecticidal activity. in the present study, de samples were collected from different locations of south-eastern europe, and their efficacy was evaluated in the laboratory against cryptolestes ferrugineus (stephens) (coleoptera: cucujiidae), sitophilus oryzae (l.) (coleoptera: curculionidae) an ...200919544360
karyology of parthenogenetic weevils (coleoptera, curculionidae): do meiotic prophase stages occur?we investigated the cytological mechanism of parthenogenesis by analyzing the chromosomes in five weevil species. all examined species are polyploids, four of which: otiorhynchus ovatus, simo variegates, cathormiocerus aristatus, and tropiphorus elevatus possess three haploid sets of chromosomes (3n=33), whereas the fifth, trachyphloeus parallelus, is tetraploid with 44 chromosomes (4n=44). the plates contained 27-31 chromosomes in triploid species and 38, 39, 41 and even 44 in tetraploid t. par ...200919595603
potential of "lure and kill" in long-term pest management and eradication of invasive species."lure and kill" technology has been used for several decades in pest management and eradication of invasive species. in lure and kill, the insect pest attracted by a semiochemical lure is not "entrapped" at the source of the attractant as in mass trapping, but instead the insect is subjected to a killing agent, which eliminates affected individuals from the population after a short period. in past decades, a growing scientific literature has been published on this concept. this article provides ...200919610395
relative susceptibility of haeckeliania sperata (hymenoptera: trichogrammatidae) to pesticides used in citrus and ornamental systems in florida.haeckeliania sperata pinto is an egg-endoparasitoid of diaprepes abbreviatus l. (coleoptera: curculionidae). we evaluated the relative susceptibility of h. sperata adults to commercially relevant pesticides used in citrus and ornamental production systems. parasitoids were exposed to pesticide residues on leaf surfaces. four concentrations of seven pesticides were tested; concentrations for each pesticide consisted of a dilution series using the label rate for field applications as the starting ...200919610401
systemic insecticides for control of black vine weevil (coleoptera: curculionidae) in container- and field-grown nursery crops.black vine weevils, otiorhynchus sulcatus (f.) (coleoptera: curculionidae), are serious pests of container- and field-grown nursery crops. management programs usually target the larval stage in container-grown plants and the adults in field-grown plants. we tested several new systemic insecticides for efficacy against black vine weevil in container-grown sedum spp. and field-grown taxus spp. in 2006 and 2007, clothianidin, dinotefuran, and chlorantraniliprole were applied as surface drenches to ...200919610404
arrestment behavior in the polyphagous tropical root weevil diaprepes abbreviatus.a semiochemical-based attractant for the tropical root weevil, diaprepes abbreviatus l., has been sought since the introduction of this polyphagous pest to florida in the 1960s. a six-unit wind tunnel apparatus was constructed to allow multiple runs to be conducted simultaneously to test the response of d. abbreviatus adults to volatile and contact semiochemicals. arrestment was measured as the number of weevils stopping and remaining on a target when presented with upwind target choices. no res ...200919610412
biological characteristics of cionus latefasciatus (coleoptera: curculionidae) and effects of temperature on its growth and development.cionus latefasciatus voss (coleoptera: curculionidae) is one of the most important insect pests on scrophularia ningpoensis hemsl. (scrophulariales: scrophuhrhceae), which is a well-known traditional chinese medicine plant in southeast china. the biological characteristics and effects of temperature on the growth and development of c. latefasciatus were studied in nature, in the greenhouse, and in the laboratory. in the area studied, 9-12 generations of this weevil occurred per year depending on ...200919610417
efficacy of two systemic insecticides injected into loblolly pine for protection against southern pine bark beetles (coleoptera: curculionidae).we evaluated the efficacy of systemic insecticides emamectin benzoate and fipronil for preventing mortality of individual loblolly pines, pinus taeda l., as a result of attacks by southern pine bark beetles (coleoptera: curculionidae, scolytinae) for two consecutive years in mississippi (2005-2006) and alabama (2006-2007). trees were injected once in the spring of 2005 (mississippi) or 2006 (alabama) and then were baited with species-specific bark beetle lures several weeks later. the southern p ...200919610420
effects of herbivory by diaprepes abbreviatus (coleoptera: curculionidae) larvae on four woody ornamental plant species.the hypothesis that herbivory by diaprepes root weevil larvae reduces leaf gas exchange and biomass was tested on buttonwood (conocarpus erectus l.), surinam cherry (eugenia uniflora l.), mahogany (swietenia mahagoni jacq.), and pond apple (annona glabra l). for surinam cherry, net co2 assimilation, transpiration, and stomatal conductance, but not internal co2 concentration (collectively referred to as leaf gas exchange values), were 7-32% higher in noninfested than infested plants. for buttonwo ...200919610430
pyrethroid resistance mediated by enzyme detoxification in listronotus maculicollis (coleoptera: curculionidae) from connecticut.in 2009, pyrethroid resistance was confirmed for seven "annual bluegrass weevil" listronotus maculicollis kirby (coleoptera: curculionidae) adult populations from southern new england. the mechanisms responsible for conferring this resistance were unknown. in this study, topical application bioassays with bifenthrin and bifenthrin combined with synergists affecting three detoxification systems were conducted on four field-collected adult populations of l. maculicollis from connecticut to determi ...200919610439
resistance among cultivated sunflower germplasm to stem-infesting pests in the central great plains.a 7-yr field study evaluated 61 oilseed sunflower, helianthus annuus l., accessions and 31 interspecific crosses for resistance to attack by naturally occurring populations of three stem-infesting pests, the sunflower stem weevil, cylindrocopturus adspersus (leconte) (coleoptera: curculionidae); a longhorned beetle, dectes texanus leconte (coleoptera: cerambycidae); and a root boring moth, pelochrista womonana (kearfott) (lepidoptera: tortricidae), at two locations in the central great plains. g ...200919610449
development of binomial sequential sampling plans for forecasting listronotus maculicollis (coleoptera: curculionidae) larvae based on the relationship to adult counts and turfgrass damage.binomial sequential sampling plans were developed to forecast weevil listronotus maculicollis kirby (coleoptera: curculionidae), larval damage to golf course turfgrass and aid in the development of integrated pest management programs for the weevil. populations of emerging overwintered adults were sampled over a 2-yr period to determine the relationship between adult counts, larval density, and turfgrass damage. larval density and composition of preferred host plants (poa annua l.) significantly ...200919610454
purification and biochemical characterization of a specific beta-glucosidase from the digestive fluid of larvae of the palm weevil, rhynchophorus palmarum.a beta-glucosidase was purified from the digestive fluid of the palm weevil rhynchophorus palmarum l. (coleoptera: curculionidae) by chromatography on anion-exchange, gel filtration, and hydrophobic interaction columns. the preparation was shown to be homogeneous on polyacrylamide gels, beta-glucosidase is a monomeric protein with a molecular weight of 58 kda based on its mobility in sds-page and 60 kda based on gel filtration. maximal beta-glucosidase activity occurred at 55 degrees c and ph 5. ...200919611239
flight activity of sitophilus oryzae (l) and sitophilus zeamais motsch (coleoptera: curculionidae) and its relationship with susceptibility to insecticides.insect-pest mobility can influence insect susceptibility to the insecticides used to control them. the objective of this work was to evaluate the flight activity of sitophilus oryzae (l) and sitophilus zeamais motsch populations reared on corn and wheat grains, and its relationship with insecticide susceptibility. unsexed adult insects with ages between 10 and 20 days were placed on a plastic tray. a 40-watt incandescent light bulb was positioned at a 50 cm height relative to the tray. flight ac ...200919618059
comparative acute toxicity of neonicotinoid and pyrethroid insecticides to non-target crayfish (procambarus clarkii) associated with rice-crayfish crop rotations.most insecticides used to control rice water weevil (lissorhoptrus oryzophilus kuscel) infestations are pyrethroids. however, pyrethroids are highly toxic to non-target crayfish associated with rice-crayfish crop rotations. one solution to the near-exclusive reliance on pyrethroids in a rice-crayfish pest management program is to incorporate neonicotinoid insecticides, which are insect specific and effective against weevils but not extremely toxic to crayfish. this study aimed to take the first ...200919623546
responses of the cabbage seedpod weevil, ceutorhynchus obstrictus (marsham) (coleoptera: curculionidae), to seed treatments of canola (brassica napus l.) with the neonicotinoid compounds clothianidin and imidacloprid.the cabbage seedpod weevil, ceutorhynchus obstrictus (marsham), is a major pest in the production of canola (brassica napus l.) in north america and europe, and effective population control is often essential for economical crop production. in north america, neonicotinoid insecticides have been used for several years in canola as seed treatments for reducing herbivory by flea beetles. the neonicotinoids clothianidin and imidacloprid were investigated to determine their effects on preimaginal dev ...200919642076
comparison of larval development and overwintering stages of the spotted knapweed biological control agents agapeta zoegana (lepidoptera: tortricidae) and cyphocleonus achates (coleoptera: curculionidae) in montana versus eastern europe.larval development of insects introduced for biological control of invasive weeds may be constrained if the new climate is more extreme than in their native range. we surveyed larval development in agapeta zoegana l. and cyphocleonus achates (fahraeus), two species of biological control insects introduced from eastern europe against spotted knapweed in western north america. we dissected spotted knapweed roots collected from five sites in western montana over 6 yr either in late fall or early sp ...200919689874
field efficacy of entomopathogenic fungi and nematodes targeting caged last-instar plum curculio (coleoptera: curculionidae) in michigan cherry and apple orchards.the plum curculio (conotrachelus nenuphar herbst) is a key pest of pome and stone fruit in eastern north america. we tested the efficacy of five pathogens over the course of three seasons in 10 michigan apple and cherry orchards, with introductions of larvae to caged pots containing pathogen-treated soil. the nematode steinernema riobrave was the most effective pathogen in the 2 yr it was tested, but only in soils with the highest sand content (81-88%) and when it was applied 1 h or 5 d after la ...200919689891
geographic distribution and regional impacts of oxyops vitiosa (coleoptera: curculionidae) and boreioglycaspis melaleucae (hemiptera: psyllidae), biological control agents of the invasive tree melaleuca quinquenervia.the invasive tree melaleuca quinquenervia (cav.) blake is widely distributed throughout peninsular florida and poses a significant threat to species diversity in the wetland systems of the everglades. mitigation of this threat includes the areawide release campaign of the biological control agents oxyops vitiosa pascoe and boreioglycaspis melaleucae moore. we summarize the results of this release effort and quantify the resulting geographic distribution of the herbivores as well as their regiona ...200919689893
take me to your leader: does early successional nonhost vegetation spatially inhibit pissodes strobi (coleoptera: curculionidae)?the spatial influences of host and nonhost trees and shrubs on the colonization patterns of white pine weevil pissodes strobi (peck) were studied within a stand of planted interior hybrid spruce [picea glauca (moench) voss x picea engelmannii (parry) ex engelm.]. planted spruce accounted for one third of all trees within the stand, whereas the remaining two thirds were comprised of early-successional nonhost vegetation, such as alder (alnus spp.), paper birch (betula papyrifera marsh.), black co ...200919689899
host preference and attack pattern of dendroctonus rhizophagus (coleoptera: curculionidae: scolytinae): a bark beetle specialist on pine regeneration.pine seedlings and saplings are seldom attacked by bark beetles of the genus dendroctonus. however, dendroctonus rhizophagus (coleoptera: curculionidae: scolytinae) thomas and bright specifically attacks pine seedlings and causes conspicuous mortality in naturally regenerated stands in the sierra madre occidental, northern mexico. we evaluated the host preference and attack of d. rhizophagus under field conditions. we tried to establish any relationship between tree growth or host size and the n ...200919689900
genetic structure of japanese populations of xylosandrus brevis (curculionidae: scolytinae).we examined the genetic structure of populations of an ambrosia beetle, xylosandrus brevis (eichhoff) (curculionidae: scolytinae), to understand its colonization dynamics. we collected specimens from 20 sites in japan and studied the genetic structure of these populations using portions of the mitochondrial cytochrome oxidase i gene (coi). a phylogenetic analysis showed three distinct lineages (clades a, b, and c) within x. brevis. clade a had 65 haplotypes from all the populations, except for p ...200919689915
dna taxonomy and phylogeography of beetles of the falkland islands (islas malvinas).the falkland biota are generally considered to be derived from the nearest continental source in patagonian south america, yet they harbor many endemic species whose taxonomy and evolutionary history remains insufficiently understood. comprehensive sampling of coleoptera over two field seasons from numerous sites across the falkland archipelago produced representatives of 55 morphologically separable species, assigned to 35 genera and 13 families of coleoptera. partial mitochondrial cytochrome o ...200919723584
do arms races punctuate evolutionary stasis? unified insights from phylogeny, phylogeography and microevolutionary processes.one of the major controversies in evolutionary biology concerns the processes underlying macroevolutionary patterns in which prolonged stasis is disrupted by rapid, short-term evolution that leads species to new adaptive zones. recent advances in the understanding of contemporary evolution have suggested that such rapid evolution can occur in the wild as a result of environmental changes. here, we examined a novel hypothesis that evolutionary stasis is punctuated by co-evolutionary arms races, w ...200919732333
effect of planting date on lissorhoptrus oryzophilus (coleoptera: curculionidae) density-yield relationship on rice in southeastern texas.results of planting date and insecticide efficacy experiments targeting lissorhoptrus oryzophilus kuschel (coleoptera: curculionidae) on rice, oryza sativa l., in southeastern texas between 2002 and 2007 were used to determine density-yield relationships as a function of planting date. soil core samples were collected on two dates during main crop development to estimate immature l. oryzophilus populations followed by main and ratoon crop harvests. analysis of covariance (ancova) revealed that p ...200919736766
on automatic bioacoustic detection of pests: the cases of rhynchophorus ferrugineus and sitophilus oryzae.the present work reports research efforts toward development and evaluation of a unified framework for automatic bioacoustic recognition of specific insect pests. our approach is based on capturing and automatically recognizing the acoustic emission resulting from typical behaviors, e.g., locomotion and feeding, of the target pests. after acquisition the signals are amplified, filtered, parameterized, and classified by advanced machine learning methods on a portable computer. specifically, we in ...200919736784
behavioural responses of the maize weevil, sitophilus zeamais, to host (stored-grain) and non-host plant volatiles.four-arm olfactometer bioassays were conducted to assess the behavioural responses of the adult maize weevil, sitophilus zeamais (motschulsky) (coleoptera: curculionidae), to harvested seeds of host plants, i.e. white maize, yellow maize (zea mays l.) and winter wheat (triticum aestivum l.) (poaceae), and non-host plant materials, i.e. alligator pepper, aframomum melegueta (rosk) k. schum (zingiberaceae), rhizomes of ginger, zingiber officinale (roscoe) (zingiberaceae), and west african black pe ...201019760598
genetic constitution of parthenogenetic form of polydrusus inustus (coleoptera: curculionidae)--hints of hybrid origin and recombinations.asexual reproduction among insects may arise spontaneously, as a result of infectious agents or hybridization between phylogenetically distinct organisms. polyploidy, which may be the result of auto- or allopoliploidization (also associated with hybridization), is often connected with parthenogenesis. however, all studied parthenogens of weevils were apomictic, rare meiotic stages have been observed in a few of them what could suggests in their genomes besides the mutational changeability also r ...200919777958
effects of biological control of rice weevil by anisopteromalus calandrae with a population of two aspergillus spp.in this study, we assessed the interaction occurring between the rice weevil and two storage molds (aspergillus candidus link and aspergillus niger van tiegem) that prefer different moisture regimens under rice storage conditions. rice weevil induced rapid population growth in both of the storage molds. the colony forming units (cfus) of a. candidus and a. niger peaked at 10(9.00 +/- 0.02) and 10(7.72 +/- 0.03)/g, respectively. all of the rice grains were infested with the molds and eventually d ...200919791605
evidence of contact pheromone use in mating behavior of the raspberry weevil (coleoptera: curculionidae).numerous studies of insect species have shown that a subset of female cuticular hydrocarbons is used as short-range or contact pheromones. here, we studied the possible use of contact pheromones in the mating behavior of the weevil aegorhinus superciliosus, a native species of chile. males mounted females only after antennal contact with the female's cuticle, and only 33% of the males attempted to mate with dead females washed with solvent. when a glass rod (dummy) was coated with female cuticul ...200919791614
pepper weevil attraction to volatiles from host and nonhost plants.the location of wild and cultivated host plants by pepper weevil (anthonomus eugenii cano) may be aided by visual cues, the male-produced aggregation pheromone, herbivore-induced, or constitutive host plant volatiles. the attractiveness of constitutive plant volatiles to pioneer weevils is important in understanding, and perhaps controlling, dispersal of this insect between wild and cultivated hosts. ten-day-old male and 2- and 10-day-old female weevils were tested in short-range y-tube assays. ...200919791617
[species diversity of ground-dwelling beetles on northern slope of daba mountain, china].by using pitfall trapping, the species composition and quantitative distribution of ground-dwelling beetles in man-made plantations in forest farm (mpff), secondary natural forests in farmland (sff), and man-made plantations in farmland (mpf) on northern slope of daba mountain were analyzed, and detrended correspondence analysis and cluster analysis were adopted to study the similarity of the species composition and quantitative distribution of the beetles among the three forest types. 1094 indi ...200919795659
biological control of the red palm weevil, rhynchophorus ferrugineus (olivier) (coleoptera: curculionidae) by the parasitoid mite, rhynchopolipus rhynchophori (ewing) (acarina: podapolipidae).the parasitoid mite, rhynchopolipus rhynchophori was released against the red palm weevil rhynchophorus ferrugineus stages. released of this mite applied at four levels of treatment, a (30 parasitic mites: 1 prey), b (60 parasitic mites: 1 prey), c (90 parasitic mites: 1 prey) and d (120 parasitic mites: 1 prey). the parasitoid mites successfully suppressed population density of the red palm weevil, r. ferrugineus stages within two weeks. the killing of different developmental stages of the red ...200919795774
identification and metabolite profiling of sitophilus oryzae l. by 1d and 2d nmr spectroscopy.the polyphagous insect sitophilus oryzae l. (coleoptera:curculionidae) has a tremendous adaptability in feeding behaviour, making it a serious invasive pest of stored cereals. the present study identifies the metabolite composition of sitophilus oryzae (s. oryzae) using nuclear magnetic resonance (nmr) spectroscopy. assignment of 1d-proton by nmr, 1h-1h cosy, 2d-tocsy 1h-1h, had been done. amongst the various biochemically important metabolites isoleucine, valine, leucine, beta-hydroxybutyrate, ...201019814847
adult fecundity, host plant preferences, field activity and parasitism in the leaf weevil phyllobius pyri (l.) (coleoptera: curculionidae).adults of the leaf weevil phyllobius pyri (l.) (coleoptera: curculionidae) feed on a wide variety of broadleaved trees and occasionally cause severe defoliation in newly established farm woodlands. there is little information, however, on the relative susceptibility of different tree species to damage or on the habitat associations of adults and larvae of p. pyri, which might indicate the conditions that predispose trees to attack. captures of adult p. pyri in emergence and flight traps in the c ...201019814848
overwintering strategy of two weevils infesting three gorse species: when cold hardiness meets plant-insect interactions.the cold hardiness of two closely related weevil species, exapion ulicis and e. lemovicinum was studied in relation to their life cycles. these two seed-eating weevils reproduce on ulex plant species with different fruiting phenologies. e. ulicis lays eggs in spring and overwinters as an adult while e. lemovicinum lays eggs in autumn and overwinters as a larva. adult weevils were collected in natural populations of brittany (western france) and characterized with morphological and molecular tool ...201019819243
factors affecting pheromone release by scyphophorus acupunctatus (coleoptera: curculionidae).scyphophorus acupuncatus gyllenhal is considered to be the most significant pest of cultivated agaves in the world. recent studies on the chemical ecology of this weevil have shown that males release a pheromone that attracts both sexes. in this study, we investigated the effect of feeding, aging, photoperiod, and time of day on pheromone release by s. acupuncatus males under laboratory conditions. the pheromone released by individual males was sampled using solid-phase microextraction and analy ...200919825297
multidisciplinary fingerprints: forensic reconstruction of an insect reinvasion.an unexpected outbreak of boll weevils, anthonomus grandis, an insect pest of cotton, across the southern rolling plains (srp) eradication zone of west-central texas, usa, was detected soon after passage of tropical storm erin through the winter garden district to the south on 16 august 2007. the synchrony and broad geographic distribution of the captured weevils suggest that long-distance dispersal was responsible for the reinvasion. we integrated three types of assessment to reconstruct the ge ...201019828497
partial purification and characterization of trypsin-like proteinases from insecticide-resistant and -susceptible strains of the maize weevil, sitophilus zeamais.serine proteinases from three strains of sitophilus zeamais (coleoptera: curculionidae), one susceptible and two resistant to insecticides--one exhibiting fitness cost (resistant cost strain) and the other lacking it (resistant no-cost strain), were partially purified using an aprotinin-agarose affinity column providing purification factors ranging from 36.5 to 51.2%, with yields between 10 and 15% and activity between 529 and 875 microm/min/mg protein with the substrate n-alpha-benzoyl-l-arg-p- ...201019835976
"candidatus curculioniphilus buchneri," a novel clade of bacterial endocellular symbionts from weevils of the genus curculio.here we investigated the bacterial endosymbionts of weevils of the genus curculio. from all four species of curculio weevils examined, a novel group of bacterial gene sequences were consistently identified. molecular phylogenetic analyses demonstrated that the sequences formed a distinct clade in the gammaproteobacteria, which was not related to previously known groups of weevil endosymbionts such as nardonella spp. and sodalis-allied symbionts. in situ hybridization revealed that the bacterium ...201019880647
impacts of seeding rate on interactions between rice and rice water weevils.the rice water weevil, lissorhoptrus oryzophilus kuschel, is the most widely distributed and destructive early season insect pest of rice, oryza sativa l., in the united states. economic losses result primarily from feeding by the larval stage of this insect on the roots of flooded rice plants. prior studies suggest that infestations of rice water weevil larvae are more severe at low plant densities. moreover, because feeding by the rice water weevil reduces rice plant tillering, a process parti ...200919886448
efficacy of verbenone for protecting ponderosa pine stands from western pine beetle (coleoptera: curculionidae: scolytinae) attack in california.the western pine beetle, dendroctonus brevicomis leconte (coleoptera: curculionidae: scolytinae), is a major cause of ponderosa pine, pinus ponderosa dougl. ex laws., mortality in much of western north america. currently, techniques for managing d. brevicomis infestations are limited. verbenone (4,6,6-trimethylbicyclo [3.1.1] hept-3-en-2-one) is an antiaggregation pheromone of several dendroctonus spp., including d. brevicomis, and it has been registered as a biopesticide for control of mountain ...200919886449
curative activity of insecticides against plum curculio (coleoptera: curculionidae) in tart cherries.tart cherry, prunus cerasus l. variety montmorency, fruit were infested with plum curculio, conotrachelus nenuphar (herbst) (coleoptera: curculionidae), and treated with insecticides to target late instars, neonates, and eggs. the organophosphates azinphos-methyl and phosmet and the neonicotinoid thiamethoxam reduced larval emergence rates by >90% for all life stage targets; after >30 d, few surviving larvae were found inside fruit. acetamiprid and thiacloprid also had curative activity and yiel ...200919886451
reconsiderations on evaluating methodology of repellent effects: validation of indices and statistical analyses.many pest repellents are in household use, and a variety of procedures, indices, and statistical tests have been used to examine their effectiveness. however, the effect of action mechanism on these indices has not been adequately discussed. in addition, the interreplication variance of data have not been taken into account in some cases. this study considered the validity of tests used to evaluate repellents and proposed methods to statistically evaluate them. first, various repellents with dif ...200919886465
volatiles from a mite-infested spruce clone and their effects on pine weevil behavior.induced responses by norway spruce (picea abies) seedlings to feeding damage by two mite species were studied by analyzing the volatiles emitted during infestation. four specimens of a norway spruce (picea abies l.) clone were infested with mites of nalepella sp., another four with oligonychus ununguis, and four were kept mite-free as controls. after a year of infestation, spruce volatiles were collected, analyzed, and identified using spme-gc-ms. in addition, enantiomers of chiral limonene and ...200919902304
field efficacy of imidacloprid and steinernema carpocapsae in a chitosan formulation against the red palm weevil rhynchophorus ferrugineus (coleoptera: curculionidae) in phoenix canariensis.the invasive red palm weevil, rhynchophorus ferrugineus (olivier), has become the major pest of palms in the mediterranean basin. chemical control against this species is difficult because of its cryptic habits and is mainly based on the repeated application of large quantities of synthetic insecticides. the aim of this work has been to evaluate in the field the efficacy of imidacloprid (confidor 240 od) and steinernema carpocapsae weiser with chitosan (biorend r palmeras) as soil and stipe trea ...201019924729
polyphyly of xylosandrus reitter inferred from nuclear and mitochondrial genes (coleoptera: curculionidae: scolytinae).the xyleborina ambrosia beetle genus xylosandrus contains 54 species, several of which are of economic importance. the monophyly of the genus was tested using a data set comprised of multiple gene loci: 28s rdna; the mitochondrial gene cytochrome oxidase i (coi); and the nuclear genes arginine kinase (argk), rudimentary (cad), and elongation factor 1alpha (ef-1alpha). the nuclear protein-coding genes cad and argk were used for the first time in phylogenetics of scolytinae. analyses were performe ...201019925873
natural selection drives the fine-scale divergence of a coevolutionary arms race involving a long-mouthed weevil and its obligate host plant.one of the major recent advances in evolutionary biology is the recognition that evolutionary interactions between species are substantially differentiated among geographic populations. to date, several authors have revealed natural selection pressures mediating the geographically-divergent processes of coevolution. how local, then, is the geographic structuring of natural selection in coevolutionary systems?200919941669
[susceptibility of the parasitoid phymastichus coffea lasalle (hymenoptera: eulophidae) to beauveria bassiana under laboratory conditions].the coffee berry borer, hypothenemus hampei (ferrari) (coleoptera: curculionidae), is the most important coffee pest worldwide. beauveria bassiana is a generalist entomopathogenic fungus widely used by coffee farmers to control this pest and phymastichus coffea lasalle (hymenoptera: eulophidae) is an african endoparasitoid of h. hampei adults, recently imported to several latin american and caribbean countries to aid in the coffee berry borer control. the objective of this study was to determine ...200919943017
purification of a defensin isolated from vigna unguiculata seeds, its functional expression in escherichia coli, and assessment of its insect alpha-amylase inhibitory activity.plant defensins make up a family of cationic antimicrobial peptides with a characteristic three-dimensional folding pattern stabilized by four disulfide bridges. the aim of this work was the purification and functional expression of a defensin from cowpea seeds and the assessment of its alpha-amylase inhibitory activity. the cdna encoding the cowpea defensin was cloned into the pet-32 ek/lic vector, and the resulting construct was used to transform escherichia coli cells. the recombinant peptide ...201019948221
the bionomics of an invasive species sitona lepidus during its establishment in new zealand.the egg, larval, pupal and adult abundance of the clover root weevil sitona lepidus gyllenhal (coleoptera: curculionidae) was monitored at three sites for the first ten years following the discovery of this exotic pest in the waikato region of new zealand. the species went through an initial boom and bust cycle at two sites, with populations reaching up to 1800 larvae m-2. thereafter, winter larval populations were relatively stable, ranging between 450-750 m-2. unlike in the northern hemisphere ...201020003608
deleterious effects of plant cystatins against the banana weevil cosmopolites sordidus.the general potential of plant cystatins for the development of insect-resistant transgenic plants still remains to be established given the natural ability of several insects to compensate for the loss of digestive cysteine protease activities. here we assessed the potential of cystatins for the development of banana lines resistant to the banana weevil cosmopolites sordidus, a major pest of banana and plantain in africa. protease inhibitory assays were conducted with protein and methylcoumarin ...201020035549
application of semiochemicals to assess the biodiversity of subcortical insects following an ecosystem disturbance in a sub-boreal forest.from 2000 through 2003 we used semiochemical-baited traps in northeastern minnesota, usa, to assess changes in assemblages of subcortical forest insects after a catastrophic wind storm in 1999 and subsequent (1999-2000) fuel-reduction activities (salvage-logging and prescribed-burning). we determined the regional efficacy of fifteen semiochemical blends (pheromones and kairomones) as attractants for target and non-target subcortical insect species (coleoptera: anthribidae, buprestidae, cerambyci ...200920066478
evaluation of multiple funnel traps and stand characteristics for estimating western pine beetle-caused tree mortality.the western pine beetle, dendroctonus brevicomis leconte (coleoptera: curculionidae: scolytinae), is a major cause of ponderosa pine, pinus ponderosa dougl. ex laws., mortality in much of western north america. this study was designed to quantify relationships between western pine beetle trap catches [including those of its primary invertebrate predator temnochila chlorodia (mannerheim) (coleoptera: trogositidae)], and levels of tree mortality attributed to western pine beetle at 44 trapping sit ...200920069846
presence and significance of bacillus thuringiensis cry proteins associated with the andean weevil premnotrypes vorax (coleoptera: curculionidae).the andean weevil premnotrypes vorax represents an important cause of damage to colombian potato crops. due to the impact of this plague on the economy of the country, we searched for new alternatives for its biological control, based on the entomopathogenic bacteria bacillus thuringiensis. a total of 300 b. thuringiensis strains obtained from potato plantations infested with p. vorax were analyzed through crystal morphology, sds-page, pcr and bioassays. we used site-directed mutagenesis to modi ...200920073348
iridoid glycoside variation in the invasive plant dalmatian toadflax, linaria dalmatica (plantaginaceae), and sequestration by the biological control agent, calophasia lunula.invasive plant species can have significant ecological and economic impacts. although numerous hypotheses highlight the importance of the chemical defenses of invasive plant species, the chemical ecology of many invasive plants has not yet been investigated. in this study, we provide the first quantitative investigation of variation in iridoid glycoside concentrations of the invasive plant dalmatian toadflax (linaria dalmatica). we examined variation in chemical defenses at three levels: (1) var ...201020077129
[attraction of sphenophorus levis vaurie adults (coleoptera: curculionidae) to vegetal tissues at different conservation levels].the occurrence of the sugarcane weevil sphenophorus levis vaurie is important in sugarcane in some regions in brazil. damage is caused by the larvae as they bore into the nodes and can reach 30 ton/ha/year. many control alternatives have been attempted, but none were satisfactory, except for the use of toxic baits. therefore, it is necessary to optimize their efficiency or to propose new techniques. the objective of the current study was to evaluate the attractiveness of adults of s. levis to su ...200920098932
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