Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| interactions with lectins and agglutination profiles of clinical, food, and environmental isolates of listeria. | on the basis of preliminary trials with 14 collection strains of listeria, five lectins (canavalia ensiformis, concanavalin a; griffonia simplicifolia lectin i; helix pomatia agglutinin; ricinus communis agglutinin; and triticum vulgaris wheat germ agglutinin) were selected to set up a microtiter agglutination assay. the lectin agglutination profiles of 174 clinical, food, and environmental strains of listeria monocytogenes, listeria innocua, and listeria seeligeri were investigated. data on the ... | 1994 | 7883879 |
| hyperplasia, hypertrophy, and phenotypic alterations in the distal nephron after acute proximal tubular injury in the rat. | little is known about the impact of acute proximal tubular injury and dysfunction on the distal nephron. | 1994 | 7909858 |
| glycosylation patterns of the human colon cancer cell line ht-29 detected by helix pomatia agglutinin and other lectins in culture, in primary tumours and in metastases in scid mice. | human colonic cancer cells (ht-29, 10(7) cells/dose) were injected subcutaneously between the scapulae of 19 severe combined immunodeficient (scid) mice. after 19 days, large tumours had developed in 18 out of the 19 animals and the mice were then killed. metastases were detected in the lungs of 16 animals but not in other organs investigated. surgical removal of the primary tumour in another group of five animals led to a prolonged survival and further growth of metastases in the lungs. ht-29 i ... | 1994 | 7923992 |
| role of cell surface o-linked oligosaccharides in adhesion of hl60 cells to fibronectin: regulation of integrin-dependent cell adhesion by o-linked oligosaccharide elongation. | the adhesion of the myelogenous leukemia cell line, hl60, to fibronectin and its fragments, heparin binding fragment (40 kda) and cell attachment fragment (120 kda), was enhanced by culturing with benzyl-alpha-galnac (bz alpha galnac). enhancement of cell adhesion to fibronectin was also observed on treatment of hl60 cells with 12-o-tetradecanoylphorbol 13-acetate (tpa). however, an additive effect of bz alpha galnac and tpa treatments was not observed. the expression of vla4 and vla5 did not ch ... | 1994 | 7925647 |
| comparative aspects of neoplastic invasion of the brain. | this review of the invasion of the human brain by tumors is based upon a comparative approach. the taxonomic distribution of a structure of the cns to be considered a brain is present in the following taxonomic groups: mollusca (cephalopoda, pulmonata) sipunculida, echiurida, annelida, arthropoda and vertebrata (fishes, amphibians, reptiles, birds and mammals). the comparative approach (inter-, and intraspecies specific) provides a more informative, indicative, understanding of invasion of the h ... | 1994 | 7964978 |
| helix pomatia agglutinin binding is a useful prognostic indicator in colorectal carcinoma. | most deaths from colorectal carcinoma are due to metastases. a relatively reliable prognostic indicator at surgery to date is the dukes' stage, but this is a morphologic approach that does not elucidate biochemical changes to explain why cells became metastatic. the binding sites for the lectin from the roman snail helix pomatia (hpa) were shown to be good prognostic indicators in breast and gastric cancer, and accordingly, this study was performed to evaluate the use of hpa binding sites as pro ... | 1994 | 7982174 |
| galactose-containing glycoconjugates of the ciliary body and lens in capsular glaucoma: a lectin histochemical study. | this study was carried out in order to obtain information on galactose-containing glycoconjugates in the lens and ciliary body of human eyes with capsular glaucoma and thus on the etiopathogenesis of exfoliation syndrome. | 1994 | 8001826 |
| differential lectin binding of onchocerca lienalis and onchocerca ochengi infective larvae following their development in simulium ornatum s.l. | lectins have been used to investigate species specific differences in carbohydrate moieties on the surface of the infective larvae of two onchocerca species following their development in simulium ornatum. of the seven fitc-labelled lectins used in this study only two, arachis hypogea (pna) and helix pomatia (hpa), bound to the surface of o. lienalis, whereas no lectin binding could be detected on the surface of o. ochengi infective larvae. this indicates that in principal lectins might be a pot ... | 1994 | 8066375 |
| alpha-n-acetylgalactosaminidase deficiency with mild clinical manifestations and difficult biochemical diagnosis. | two additional patients with alpha-n-acetylgalactosaminidase (alpha-naga) deficiency are described. an 11-month-old girl with nonconsanguineous parents had generalized seizures and no angiokeratoma. biochemical investigation showed persistent slight oligosacchariduria; enzymatic analysis of plasma, leukocytes, and fibroblasts revealed profound alpha-naga deficiency. heterozygote enzyme levels were found in both parents. the mother has epilepsy, and epilepsy is present in the father's family. a y ... | 1994 | 8071745 |
| prognostic significance of helix pomatia lectin and c-erbb-2 oncoprotein in human breast cancer. | we investigated the prognostic significance of helix pomatia lectin (hpa) staining on disease-free and overall survival in 120 primary breast carcinomas. hpa staining was present in 58 (48%) of these carcinomas. it was significantly associated with axillary lymph node metastases (p < 0.001) and c-erbb-2 expression (p < 0.01). a univariate study revealed that disease-free and overall survival were significantly correlated with clinical stage, tumour size, axillary lymph node metastases. hpa stain ... | 1993 | 8102537 |
| immunocytochemical distribution of neuropeptide f (npf) in the gastropod mollusc, helix aspersa, and in several other invertebrates. | the distribution of neuropeptide f (npf) immunoreactivity in the snail, helix aspersa, has been demonstrated by immunocytochemistry using 2 region-specific antisera. one, designated npf3, was raised against a synthetic n-terminal fragment of helix aspersa npf; the other, designated pp221, was raised against the c-terminal hexapeptide amide of mammalian pancreatic polypeptide (pp) but cross-reacts fully with the analogous c-terminal region of helix aspersa npf. the distribution of npf immunoreact ... | 1994 | 8111844 |
| identification of a major poly-n-acetyllactosamine-containing cell-surface glycoprotein of mouse teratocarcinoma cells. appearance on cells induced to primitive endoderm but not parietal endoderm differentiation. | mouse teratocarcinoma f9 cells were induced to primitive endoderm differentiation with retinoic acid, and poly-n-acetyllactosamine-containing surface glycoproteins were identified by radiolabelling endo-beta-galactosidase-cleavable glycans with galactosyltransferase and radiolabelled udp-galactose. one major radiolabelled band with an apparent size of 250-500 kda was identified which differed from the known poly-n-acetyllactosamine-containing glycoproteins laminin, fibronectin, lysosome-associat ... | 1994 | 8125095 |
| interaction of hamster submaxillary sialyl-tn and tn glycoproteins with gal, galnac and glcnac specific lectins. | hamster submaxillary glycoprotein (hsm), one of the simplest glycoproteins among mammalian salivary mucins, is composed of approximately equivalent amounts of protein, hexosamine and sialic acid. the thr and ser residues in the protein core account for more than half of all of the amino acid residues, while lys, glu, pro and ala are the major components of the remaining portion of amino acids. the carbohydrate side chains of this mucous glycoprotein have mainly the neuac-galnac-(sialyl-tn) seque ... | 1994 | 8183285 |
| helix pomatia agglutinin binding in human tumour cell lines: correlation with pulmonary metastases in nude mice. | the extent of lectin binding by three human melanoma (lox, femx-1 and sesx) and two sarcoma lines (mhmx and ohsx) was related to their potential for experimental metastasis formation in athymic nude mice. the helix pomatia agglutinin (hpa), which recognises the n-acetyl-d-galactosamine ligand, showed differential binding to the cell lines in a manner that correlated with their ability to give lung colonies after i.v. injection in the mice (p < 0.005). the degree of hpa binding and lung colony fo ... | 1994 | 8198963 |
| structural organization of the sensory systems of the snail. | the author's data on the structural organization of the nervous system of the body wall, the gravitational organs (the statocysts, the eyes, and the chemosensory organs), and the head tentacles of the snails helix vulgaris and helix pomatia are correlated in this paper. the localization in the cns and some structural characteristics of the central divisions of the sensory systems are shown. information is presented on the structure of the procerebrum, an associative center participating in the p ... | 2008 | 8208381 |
| [long term survival of first stage larvae of elaphostrongylus cervi (nematoda, metastrongyloidea) in a frozen state]. | faeces of red deer from białowieza forest containing first stage larvae of elaphostrongylus cervi have been stored during five years in -20 degrees c. after this time 52% of the larvae survived. these larvae were fully alive and in experimentally infected snails helix pomatia developed to the infectious stage. | 1993 | 8237006 |
| differentiation of pancreatic acinar cells into duct-like cells in vitro. | previous studies have shown that a combination of both the extracellular matrix and secretagogues plays critical roles in the maintenance of well-differentiated pancreatic acinar cells in culture. in the present study, we have shown that, upon proper stimulation, acinar cells change their growth pattern and morphologic appearance to a duct like phenotype. | 1993 | 8246444 |
| histochemistry with helix pomatia agglutinin in human germ cell tumors: detection of nongerminomatous components and correlation between hpa reactivity and radiosensitivity in germinomas. | binding sites of helix pomatia agglutinin (hpa) were examined in 32 patients with intracranial human germ cell tumors. hpa reactivity was found in vascular endothelial cells and erythrocytes of patients with blood type a or ab. hpa-positive neoplastic cells were seen in one yolk sac carcinoma in a patient with blood group a, and in embryonal carcinomas and teratomas irrespective of blood group type. although in 10 out of 18 germinomas neoplastic cells were totally negative for hpa, another 8 ger ... | 1993 | 8252515 |
| developmental changes in neutral glycosphingolipids of mouse placenta. | the mammalian placenta is a unique organ for the study of developmental changes. placentas of laboratory animals such as the mouse allow for the determination of the exact stage of pregnancy, which cannot be achieved with human placenta. in this study, neutral glycosphingolipids were isolated from mouse (inbred strain c57bl/6) placentas, from day 10 to day 18 of gestation, and were separated by high performance thin layer chromatography. densitometric measurements after orcinol staining showed, ... | 1993 | 8257854 |
| affinity methods with lectins: a tool to identify canine alkaline phosphatase isoenzymes. | affinity methods were used to characterize selective interactions of alkaline phosphatase (alp) isoenzymes from different dog tissues with lectins. specific lectins were used to identify liver, intestinal and steroid-induced alp isoenzymes in serum from dogs with cushing syndrome or steroid-treated dogs. for the first approach, 12 lectins were assayed by affinity dots. selective interactions were found among wheat germ agglutinin (wga), jacalin, con a (concanavalin a) and helix pomatia agglutini ... | 1993 | 8258637 |
| histochemical analysis of rat testicular glycoconjugates. 3. non-reducing terminal residues in seminiferous tubules. | lectins of helix pomatia (hpa), glycine max (sba), vicia villosa (vva), dolichos biflorus (dba), ulex europaeus (uea-1), tetragonolobus purpureus (lta), griffonia simplicifolia (bsa-1b4), maclura pomifera (mpa), sambucus nigra (sna) and maackia amurensis (maa) were used to explore the distribution of saccharides characteristic of non-reducing termini of o- and n-linked glycoprotein glycans in the seminiferous tubules of rat testis. sialyl residues (both alpha 2,3- and alpha 2,6-linked, as shown ... | 1993 | 8282567 |
| radioimmunoassay of 2-hydroxyestrone in urine. | catechol estrogens such as 2-oh estrone are interesting estrogen metabolites formed in several human tissues and excreted in urine. we developed and thoroughly validated a radioimmunoassay for urinary 2-oh estrone that has several advantages over published rias. because we developed a relatively specific antiserum, we did not include a preliminary chromatographic step to eliminate cross-reacting urinary steroids. we hydrolyzed urinary steroid conjugates with beta-glucuronidase from helix pomatia ... | 1994 | 8287549 |
| interaction of a novel tn (galnac alpha 1-->ser/thr) glycoprotein with gal, galnac and glcnac specific lectins. | a naturally occurring tn glycoprotein (native asg-tn) with galnac alpha 1-->ser/thr as the only carbohydrate side chains, has been prepared from armadillo submandibular glands. in a quantitative precipitin assay, this glycoprotein completely precipitated maclura pomifera (mpa), vicia villosa b4 (vvl-b4) and artocarpus integrifolia (jacalin, ail). it also reacted well with helix pomatia (hpl) and wistaria floribunda (wfl) and precipitated over 75% of the lectin nitrogen added, but poorly with ric ... | 1994 | 8292029 |
| characterization of the budding compartment of mouse hepatitis virus: evidence that transport from the rer to the golgi complex requires only one vesicular transport step. | mouse hepatitis coronavirus (mhv) buds into pleomorphic membrane structures with features expected of the intermediate compartment between the er and the golgi complex. here, we characterize the mhv budding compartment in more detail in mouse l cells using streptolysin o (slo) permeabilization which allowed us to better visualize the membrane structures at the er-golgi boundary. the mhv budding compartment shares membrane continuities with the rough er as well as with cisternal elements on one s ... | 1994 | 8294506 |
| heterogeneity of human gallbladder mucin in bile. | 1. human gallbladder mucin has been implicated to play a role in gallstone disease. in spite of this fact relatively little is known about the structure of human gallbladder mucin. in this study we have investigated the possible heterogeneity of mucin. for this purpose polyclonal and monoclonal antibodies against gallbladder mucin were raised. all antibodies reacted primarily with carbohydrate antigenic determinants. with these antibodies the immunoreactivity of gallbladder mucin from 60 patient ... | 1994 | 8306554 |
| lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man. | the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ... | 1993 | 8310810 |
| lectin histochemistry of normal and neoplastic peripheral nerve sheath. 2. lectin binding patterns of schwannoma and neurofibroma. | lectin binding patterns of 31 schwannomas and 6 neurofibromas were examined using 12 lectins, and the results were compared with those of normal peripheral nerves. tumors obtained from 10 cases of neurofibromatosis and 4 recurrent schwannomas were included. changes of glycoconjugates were observed in association with a neoplastic transformation of schwann cells; arachis hypogaea (pna) staining after neuraminidase treatment seen in normal schwann cells was reduced in schwannoma of antoni type a, ... | 1993 | 8310811 |
| the use of lectins identified with specific antibodies in lectin histochemistry of nzb/w f1 mouse kidney. | the affinity of helix pomatia, peanut, pisum sativum, soy bean, and wheat germ agglutinins to various nephron parts of nzb/w f1 mice was different and is assumed to be age dependent. the affinity of pisum sativum agglutinin to basal membranes of small renal vessels increased with the age of nzb/w f1 mice. the wheat germ agglutinin bound to structures with alkaline phosphatase activity. | 1993 | 8351979 |
| neurons in a variety of molluscs react to antibodies raised against the vd1/rpd2 alpha-neuropeptide of the pond snail lymnaea stagnalis. | the vd1 and rpd2 neurons of lymnaea stagnalis innervate other central neurons, certain skin areas, the pneumostome area, and the auricle of the heart. recently, a set of four (delta, epsilon, alpha, beta) neuropeptides produced by these giant neurons and by certain other central neurons has been characterized. although alternative splicing of the preprohormone of these neurons yields at least 10 different alpha neuropeptides, an affinity-purified antiserum directed against a domain common to all ... | 1993 | 8364974 |
| fragmentation and dispersal of golgi proteins and redistribution of glycoproteins and glycolipids processed through the golgi apparatus after infection with herpes simplex virus 1. | in vero monkey cells and hep-2 human epidermoid carcinoma cells infected with herpes simplex virus 1 the proteins beta-cop, galactosyltransferase, and alpha-mannosidase ii associated with the golgi apparatus appear to be associated with numerous smaller structures dispersed throughout the cytoplasm. concomitantly, the intracytoplasmic ligands of lectins normally associated wholly (helix pomatia or ricinus communis agglutinin) or in part (wheat germ agglutinin) with the golgi apparatus increased ... | 1993 | 8385343 |
| immune-mediated alteration of the terminal sugars of goblet cell mucins in the small intestine of nippostrongylus brasiliensis-infected rats. | alteration of terminal sugars of goblet cell mucins in the small intestinal mucosa was examined by lectin histochemistry in rats infected with nippostrongylus brasiliensis. to see whether alteration of the nature of mucins was regulated by the local immune system, adult worms were implanted intraduodenally into recipient rats of various immune status. when 7-day-old ('normal') adult worms were implanted into naive euthymic rats, about 60% remained until day 7 and then were expelled. the number o ... | 1993 | 8473019 |
| cytochemical characterization of basement membranes in the enamel organ of the rat incisor. | ameloblasts are unique epithelial cells, in that once they have deposited the entire thickness of enamel and the process of maturation begins, they reform a basal lamina-like structure at their apical surface. in order to characterize further this basal lamina, its composition was analysed using (1) lectin-gold cytochemistry for glycoconjugates, (2) high-iron diamine (hid) staining for sulfated glycoconjugates and (3) immunogold labeling for collagen type iv and laminin. the labeling patterns we ... | 1993 | 8500995 |
| carbohydrate and peptide antigens in macrophage populations derived from human bone marrow and milk: an immunomorphological and immunochemical analysis. | an immunomorphological and immunochemical study was performed to elucidate the pattern of carbohydrate antigens and their relationships to the cluster differentiation (cd) 68 epitopes on macrophages derived from human bone marrow and milk. core and backbone antigens recognized by lectins from bauhinia purpurea (bpa), helix pomatia (hpa), arachis hypogaea (pna), glycine max. (sba), griffonia simplicifolia (gsa-i-b4), lycopersicon esculentum (lea) and erythrina cristagalli (eca) were expressed by ... | 1995 | 8550384 |
| intermediate forms of glycoconjugates are present in the envelope of herpes simplex virions during their transport along the exocytic pathway. | in cells infected with herpes simplex virus 1, intracellular virions in transit along the exocytic pathway carry glycoconjugates that react, in fracture-label technique, with helix pomatia lectin. this lectin is specific for unsubstituted n-acetylgalactosamine, an intermediate sugar added in o-linkage to ser/thr residues in cis-golgi and then substituted with galactose and sialic acid in the trans-golgi. virions in the perinuclear space do not react with helix pomatia lectin. in intracellular tr ... | 1995 | 8553564 |
| lectin binding to extracellularly melanized microfilariae of brugia malayi from the hemocoel of anopheles quadrimaculatus. | binding patterns of fluorescein isothiocyanate (fitc)- and gold-conjugated lectins to extracellularly melanized sheathed and exsheathed microfilariae of subperiodic brugia malayi, isolated from and in situ in the abdominal hemocoel of anopheles quadrimaculatus 72-hr postinfection, were examined. five fitc-conjugated lectins [helix pomatia agglutinin (hpa), arachis hypogaea (peanut agglutinin-pna), triticum vulgaris (wheat germ agglutinin-wga), lens culinaris (lentil-lch), and concanavalin a (con ... | 1995 | 8568282 |
| growth related changes in sugar determinants on the surface of c6 glioma cells in culture: a cytochemical lectin-binding study. | the cell surface sugar determinants (cssd) were examined in c6 glioma cells in cultures at different conditions of growth by peroxidase conjugates of the lectins: peanut agglutinin (pna), ricinus communis agglutinin (rca), helix pomatia agglutinin (hpa), wheat germ agglutinin (wga), lentil agglutinin (lca), laburnum bork agglutinin (laba), and lotus agglutinin (tpa). it was found that the cells bound more intensively wga, lca, and rca compared to pna, hpa; the weakest staining was provided by la ... | 1995 | 8568919 |
| blood group glycolipids as epithelial cell receptors for candida albicans. | the role of glycosphingolipids as possible epithelial cell receptors for candida albicans was examined by investigating the binding of biotinylated yeasts to lipids extracted from human buccal epithelial cells and separated on thin-layer chromatograms. binding was visualized by the addition of 125i-streptavidin followed by autoradiography. five c. albicans strains thought from earlier work to have a requirement for fucose-containing receptors all bound to the same three components in the lipid e ... | 1996 | 8641797 |
| metallothionein research in terrestrial invertebrates: synopsis and perspectives. | while most of metallothionein research during the past years has been carried out on mammals or vertebrates, only relatively few studies have been directed towards invertebrates. even fewer investigations have focussed on terrestrial invertebrates. the best studied metallothioneins and/or metallothionein genes among terrestrial invertebrates are those from an insect species (drosophila melanogaster), a nematode (caenorhabditis elegans) and some terrestrial gastropods (helix pomatia, arianta arbu ... | 1996 | 8646613 |
| lectin histochemistry of pregnant rat uterine tissues. | glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. a wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from canavalia ensiformis (con a), lens culinaris (lch) culinaris (lch), pisum sativum (psa), griffonia simplicifolia ii (gs-ii), triticum vulgaris (wga), griffonia simplicifolia ... | 1996 | 8655407 |
| a histological, lectin and s-100 histochemical study of the developing prenatal human sublingual salivary gland. | lectin and s-100 protein histochemistry during fetal growth and development (10-38th gestational weeks) of these glands was studied. the histological development of glandular structures followed the known pattern for other salivary glands. using biotinylated lectins ulex europeus-i, dolichos biflorus, glycine maximus (soyabean), helix pomatia, arachis hypogaea (peanut) and triticum vulgare (wheatgerm), the binding level and, by implication, the concentration of associated specific oligosaccharid ... | 1995 | 8670026 |
| binding properties of a blood group le(a+) active sialoglycoprotein, purified from human ovarian cyst, with applied lectins. | studies on the structures and binding properties of the glycoproteins, purified from human ovarian cyst fluids, will aid the understanding of the carbohydrate alterations occurring during the biosynthesis of blood group antigens and neoplasm formation. these glycoproteins can also serve as important biological materials to study blood group a, b, h, le(a), le(b), le(x), le(y), t and tn determinants, precursor type i and ii sequences and cold agglutinin i and i epitopes. in this study, the bindin ... | 1996 | 8672551 |
| a new subgroup of lectin-bound biliary proteins binds to cholesterol crystals, modifies crystal morphology, and inhibits cholesterol crystallization. | biliary proteins inhibiting or promoting cholesterol crystallization are assumed to play a major role in cholesterol gallstone pathogenesis. we now report a new group of biliary proteins that bind to cholesterol crystals, modify crystal morphology, and inhibit cholesterol crystallization. various glycoprotein mixtures were extracted from abnormal human gallbladder bile using lectin affinity chromatography on concanavalin a, lentil, and helix pomatia columns and were added to supersaturated model ... | 1995 | 8675674 |
| changes in keratinocyte differentiation during accelerated repopulation of the irradiated mouse epidermis. | epidermal stem cells accelerate their repopulation rate during fractionated irradiation. to study the changes in keratinocyte differentiation associated with radiation-induced repopulation, we investigated the expression of a panel of 14 monoclonal or polyclonal antibodies against keratins, involucrin and others, as well as the expression of 15 lectins in the irradiated mouse leg skin. tissue samples were collected after 1, 2, and 3 weeks of daily irradiation with 3 gy per fraction. abnormal mor ... | 1996 | 8691028 |
| characterization of the mucins produced by normal human colonocytes in primary culture. | mature goblet cells filled with mucin ready for secretion represent about one third of the cells in primary cultures of human colonocytes. in the present study characterization of the mucins produced by cultured human colonocytes was made by histochemical methods by lectin and monoclonal antibody binding. two monoclonal antibodies and three lectins (dolichos biflorus (dba), helix pomatia (hpa) and arachis hypogea (pna) recognizing epitopes or sugar haptens characteristic of different stages of m ... | 1996 | 8739831 |
| detection of dihydrotestosterone (dht) doping: alterations in the steroid profile and reference ranges for dht and its 5 alpha-metabolites. | dihydrotestosterone (dht), a biologically active metabolite of testosterone, may be misused in sports to benefit from its anabolic and psychotropic effects. after dht application, a significant increase of the glucuronides of dht and its metabolites can be expected for a certain time period depending upon dose, formulation, route of administration, and in case of percutaneous administration the chainlength of the ester. dht and its metabolites can be monitored by gas-chromatography/mass spectrom ... | 1995 | 8776070 |
| glycoconjugates and keratin 18 define subsets of taste cells. | sections of neonatal, normal adult and denervated adult rat tongue were examined with lectin histochemistry. attention was focused upon intragemmal cells (cells within the taste bud) and the surrounding perigemmal cells. informative staining patterns were observed with four of 12 lectins: ulex europaeus (uea-i), bauhinia purpurea (bpa), helix pomatia (hpa) and lotus tetragonolobus (lta) agglutinins. in normal adult tongues, bpa bound to those lingual epithelial cells lacking contact with the bas ... | 1995 | 8789401 |
| lectin histochemistry in the rats brain in experimental postresuscitation syndrome. (early and late changes). | the morphological and histochemical changes in the rat brain, resulting from global cerebral ischemia due to cardiac arrest and cessation of respiratory function, connected with the disturbances of blood-brain barrier mechanisms inclined us to perform a series of studies on the localization of specific sugar residues in the membrane glycoprotein chains, using lectin techniques. chosen lectins, represented by synthetic plant glycoproteins which are specifically bound to particular sugar residues ... | 1996 | 8791896 |
| generation of in vitro natural cytotoxicity of horse lymphocytes against sarcoid-derived tumor cells not expressing major histocompatibility complex antigens. | to analyze in vitro lymphocyte-mediated immune responses of horses with sarcoids against allogeneic sarcoid cells containing endogenous retrovirus but not expressing major histocompatibility complex antigens. | 1996 | 8807009 |
| identification of beta-glucuronidase-resistant diastereomeric glucuronides of 3-hydroxy-3-methyloxindole formed during 3-methylindole metabolism in goats. | goats were jugularly infused with the pneumotoxin 3-methylindole (3mi; 15 mg/kg, 0.5 microci/kg) dissolved in cremophor-el to characterize the urinary metabolites of 3mi in a ruminant specie. urine was collected for 36 hr after the beginning of a 2-hr infusion period, and 3mi metabolites were purified using reversed-phase hplc. goats excreted 3mi as at least 11 distinct metabolites. metabolites were characterized using a combination of uv spectroscopy, 1h- and 13c-nmr spectroscopy, and negative- ... | 1996 | 8825199 |
| suitability of different staining methods for the identification of isolated and cultured cells from guinea pig (cavia aperea porcellus) stomach. | cell suspensions from the guinea pig gastric mucosa were obtained using a pronase/collagenase isolation method, and cultured on petri dishes in minimum essential medium at 37 degrees c. for proper identification of different gastric cell types in cytospots, cell suspensions or culture, selective staining methods were employed, modified and evaluated. mucous cells and mucous neck cells were detected by use of lectins. mucous cells were stained on cytospots and in primary cultures with lectins fro ... | 1995 | 8835136 |
| neurons with different immunoreactivity form clusters in the cns of helix pomatia. | the distribution of serotonin (5ht-ir), fmrf amide (fmrf a-ir), catch-relaxing-peptide (carp-ir), dopamine (da-ir), gamma-amino-butyric-acid (gaba-ir), and leucokinin (lk-ir) immunoreactive neurons were compared in the ganglia of helix cns. these neurons are not distributed randomly, but their location outlines distinct groups in the ganglia. in a few groups only da-ir, gaba-ir and lk-ir neurons can be seen, whereas in the majority of groups fmrfa-ir, carp-ir and 5ht-ir neurons are localized tog ... | 1995 | 8853698 |
| tachykinin- and leucokinin-related peptides in the molluscan nervous system. | distribution of locustatachykinin-like immunoreactive (lomtkli) and leucokinin like immunoreactive (lkli) neurons in the central nervous system (cns) of the pond snail, lymnaea stagnalis was investigated and compared to that found in helix pomatia. occurrence of lomtkli neurons in different ganglia of the freshwater bivalve, anodonta cygnea, was also studied. similar to helix, the lymnaea cns contained lomtkli and lkli neurons mainly in the cerebral and pedal ganglia, but the number of labelled ... | 1995 | 8853699 |
| guinea pig intestinal phospholipase b: protein expression during enterocyte maturation and effects of n-oligosaccharide removal on enzymatic activities and protein stability. | guinea pig phospholipase b (plb) is an intestinal brush-border hydrolase displaying a broad substrate specificity towards various dietary lipids. plb was detected by immunoblotting as a single 140-kda polypeptide in all cell populations isolated from guinea pig intestinal mucosa, but increased in parallel to its activity from undifferentiated to mature cells, the specific activity of the enzyme remaining constant. moreover, n-glycosylation, which contributed to 23% of the apparent molecular mass ... | 1996 | 8856041 |
| identification, purification and analysis of a 55 kda lectin binding glycoprotein present in breast cancer tissue. | the helix pomatia agglutinin (hpa)-binding glycoproteins from primary breast cancers and their metastases were compared with appropriate normal control tissues on western blots. from these studies a single glycoprotein of 55 kda was found to bind hpa in tumours but not in normal control tissues. the glycoprotein was identified by protein sequencing as being homologous to human immunoglobulin heavy chain variable region. subsequent immunostaining showed it to be immunoglobulin subclass a. iga1 wa ... | 1996 | 8894309 |
| localization of binding sites of ulex europaeus i, helix pomatia and griffonia simplicifolia i-b4 lectins and analysis of their backbone structures by several glycosidases and poly-n-acetyllactosamine-specific lectins in human breast carcinomas. | several studies have shown the deletion of blood group a or b antigens and the accumulation of h antigens in human breast carcinomas. other studies have independently demonstrated that the binding sites of lectins such as helix pomatia agglutinin (hpa) and griffonia simplicifolia agglutinin i-b4 (gsai-b4) are highly expressed in these cells. in order to clarify the molecular mechanisms of malignant transformation and metastasis of carcinoma cells, it is important to understand the relationship b ... | 1996 | 8897074 |
| specific effect of maleate on an apical membrane glycoprotein (gp330) in proximal tubule of rat kidneys. | maleate treatment of rats induces transport defects similar to those seen in the fanconi syndrome (glycosuria, aminoaciduria, phosphaturia, proteinuria, etc.) and causes an accumulation of apical vesicles in proximal tubule epithelial cells. because the apical membrane glycoprotein, gp330, is a receptor associated with the apical endocytotic and recycling apparatus in these cells, we examined the effect of maleate on the distribution of this protein and other brush border markers. rats received ... | 1996 | 8898022 |
| prediction of neurotoxic potency of hazardous substances with a modular in vitro test battery. | neurotoxic action was investigated on different model nervous systems linked to a modular in vitro test battery. voltage operated potassium channels and glutamate operated ion channels expressed in oocytes of the clawed frog xenopus laevis by injection of crna (cloned rna) or mrna, respectively, as well as isolated neurons and isolated neuronal networks from the buccal ganglia of the snail helix pomatia, were used as consecutive modules of different complexity. lead (pb2+) was chosen as a known ... | 1996 | 8920725 |
| lectins and antibodies against blood-group antigens as tools for studying the cellular source of glycoproteins in human bronchial fluid: a comparison of morphological and biochemical observations. | we used nine lectins and three antibodies directed against abo blood-group antigens in morphological and western-blot experiments to investigate the source of secretory products of human large airways. in tissue sections, the lectins from griffonia simplicifolia (type i b4), dolichos biflorus, and helix pomatia, and the antibodies to the a, b, and/or h-antigen bound to mucous gland cells and to goblet cells; the binding of these substances was dependent on secretor status and abo blood group. th ... | 1996 | 8929348 |
| distribution of lectin binding sites in human bone marrow. identification by use of an ultrastructural postembedding technique. | the purpose of this ultrastructural study was to detect various carbohydrate residues on mature elements of the major human haematopoietic cell lines (granulopoiesis, erythropoiesis and megakaryopoiesis), sinus endothelium and plasma cells under comparable experimental conditions. marrow specimens were processed according to a modified postembedding technique with unicryl as embedding resin. a broad panel of 10 digoxigenin (dig)-conjugated lectins was applied for staining and specificity was eva ... | 1996 | 8933737 |
| the human muc2 mucin apoprotein appears to dimerize before o-glycosylation and shares epitopes with the 'insoluble' mucin of rat small intestine. | rabbit antiserum against a synthetic peptide corresponding to a tandemly repeated amino acid sequence in the human intestinal mucin apoprotein muc2 was used in immunoprecipitation to study the biosynthesis of muc2 in the colon-carcinoma cell line ls 174t. under non-reducing conditions, two bands were precipitated, the smaller with an apparent size of about 700 kda on sds/page. when analysed by two-dimensional electrophoresis after reduction, the larger band migrated to the same position as the s ... | 1995 | 8948445 |
| the expulsion of echinostoma trivolvis from c3h mice: differences in glycoconjugates in mouse versus hamster small intestinal mucosa during infection. | mucosal glycoconjugates were examined in c3h mice and in hamster small intestines infected with echinostoma trivolvis and in uninfected rodents, using periodic-acid schiff (pas) and high-iron diamine-alcian blue (hid-ab) staining and three different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), helix pomatia agglutinin (hpa) and griffonia simplicifolia agglutinin (gsa-ii). lectin-labelling by electron microscopy was also undertaken with wga and hpa lectin-gold probes. hid-a ... | 1996 | 8960206 |
| does the lectin helix pomatia agglutinin bind to hyaluronic acid in breast and colon cancer? | the lectin helix pomatia agglutinin (hpa) has been successfully used as an indicator of metastatic spread in a number of epithelial neoplasms including breast, colorectal, gastric, prostate and oesophageal cancers. despite its use, the binding partners of hpa in tissue sections have not been defined at the molecular level. hpa has two main binding specificities: 1) n-acetylgalactosamine (galnac) and 2) n-acetylglucosamine (glucnac). in order to determine whether hpa binds to hyaluronic acid (a g ... | 1996 | 8960307 |
| transplantation of a human ovarian cystadenocarcinoma into severe combined immunodeficient (scid) mice--formation of metastases without significant alteration of the tumour cell phenotype. | human ovarian papillary cystadenocarcinoma cells were injected intraperitoneally into severe combined immunodeficient (scid) mice. after intraperitoneal application the cells, designated sotü, grew well in vivo, lodged on to the peritoneum, formed local metastatic deposits, led to the development of ascites in the mice and formed distant metastases in the lungs. if lodged in the ovary, the morphology of the sotü tumour remarkably resembled that of the primary tumour in the patient. in contrast, ... | 1996 | 8977374 |
| vasoactive intestinal peptide-stimulated glycoconjugate secretion from conjunctival goblet cells. | we have developed an in vitro and in vivo method to determine if vip-stimulates conjunctival goblet cell secretion in the rat as nerves which contain vasoactive intestinal peptide (vip) and are most likely parasympathetic are localized around these cells. for the in vitro method, pieces of rat conjunctiva were incubated for 1 hr with no additions or increasing concentrations of vip (10(-10)-10(-6)m). goblet cell secretion was measured by determining the amount of helix pomatia agglutinin (hpa)-d ... | 1996 | 8983961 |
| synapsin-like molecules in aplysia punctata and helix pomatia: identification and distribution in the nervous system and during the formation of synaptic contacts in vitro. | the distribution and biochemical features of the synapsin-like peptides recognized in aplysia and helix by various antibodies directed against mammalian synapsins were studied. the peptides can be extracted at low ph and are digested by collagenase; further, they can be phosphorylated by both protein kinase a and ca2+/calmodulin-dependent protein kinase ii. in the ganglia of both snails, they are associated with the soma of most neurons and with the neuropil; punctate immunostaining is present a ... | 1996 | 8996802 |
| experimental evidence of secretion by mouse granulated metrial gland cells. | granulated metrial gland cells are cytotoxic cells found in the uterine wall in pregnancy. in this study, the involvement of the cytoplasmic granules of mouse granulated metrial gland cells during cytolysis of fibrosarcoma wehi 164 cells was investigated in vitro. using the lectin helix pomatia agglutinin which binds to the granule membrane, but not to the cell membrane, of mouse granulated metrial gland cells, the limiting membrane of one or more cytoplasmic granules became incorporated into th ... | 1997 | 9250711 |
| galactose-deficient iga1 in sera of iga nephropathy patients is present in complexes with igg. | iga1 proteins from sera of patients with iga nephropathy (igan) are galactosylated to a lesser degree than those from healthy controls. the increased reactivity of intact or de-sialylated serum iga1 with n-acetylgalactosamine (galnac)-specific lectins, helix aspersa (haa) and caragana arborescens (caa) and de-sialylated iga1 with helix pomatia (hpa) and bauhinia purpurea (bpa) indicated that the gal deficiency is in glycans located in the hinge region of iga1 molecules. de-sialylated iga from se ... | 1997 | 9264010 |
| comparative abilities and optimal conditions for beta-glycosidase enzymes to hydrolyse the glucuronide, glucoside, and n-acetylglucosaminide conjugates of bile acids. | enzymatic hydrolyses were described for three variants of glycosidic conjugated bile acids with one beta-glucuronidase (helix pomatia), three beta-glucosidase (almonds, sweet almonds, and escherichia coli), and four beta-n-acetylglucosaminidase (jack beans, bovine kidney, human placenta, and diplococcus pneumoniae) preparations. the substrates include the beta-glucuronide, beta-glucoside, and beta-n-acetylglucosaminide conjugates of bile acids related to hyodeoxycholic, murideoxycholic, chenodeo ... | 1997 | 9300125 |
| evidence for a cysteine-histidine thioether bridge in functional units of molluscan haemocyanins and location of the disulfide bridges in functional units d and g of the betac-haemocyanin of helix pomatia. | in functional units d and g from the betac-haemocyanin of the gastropod helix pomatia, aminoacid analysis in the presence of dimethyl sulfoxide showed the occurrence of seven cysteine residues. titration with 5,5'-dithiobis(2-nitrobenzoic acid), however, did not reveal any free thiol group. pepsinolysis at ph 2.0 followed by amino acid analysis and partial sequencing of the cysteine-containing peptides showed that six cysteine residues are involved in the formation of three disulfide bridges at ... | 1997 | 9342242 |
| differential binding properties of gal/galnac specific lectins available for characterization of glycoreceptors. | differentiating the binding properties of applied lectins should facilitate the selection of lectins for characterization of glycoreceptors on the cell surface. based on the binding specificities studied by inhibition assays of lectin-glycan interactions, over twenty gal and/or galnac specific lectins have been divided into eight groups according to their specificity for structural units (lectin determinants), which are the disaccharide as all or part of the determinants and of galnac alpha 1--> ... | 1997 | 9343930 |
| localization of procollagen i in the lysosome/endosome system of human fibroblasts. | a significant amount of newly synthesized collagen is degraded intracellularly rather than secreted, but there is controversy about whether this process occurs in the lysosomes. we addressed this problem using confocal microscopy and immunofluorescence imaging to study the distribution of procollagen i in the golgi and the lysosome/endosome system of cultured human fibroblasts. cells were incubated under basal conditions and then permeabilized and exposed to fluorescently tagged probes for proco ... | 1997 | 9344594 |
| primary structure of 21 novel monoantennary and diantennary n-linked carbohydrate chains from alphad-hemocyanin of helix pomatia. | the primary structures of 21 novel monoantennary and diantennary n-glycans of the glycoprotein alphad-hemocyanin (alphad-hc) of helix pomatia have been determined. outer oligosaccharide fragments (antennae) were released from the glycoprotein by smith degradation of an alphad-hc pronase digest. the major antenna, obtained following hplc fractionation on lichrosorb-nh2, was characterized using 1h-nmr spectroscopy, fast-atom-bombardment mass spectrometry, and linkage analysis, and corresponds to a ... | 1997 | 9363772 |
| lectins and antibodies to blood group antigens as markers for the basal cells of the human respiratory epithelium. | we used a pattern of 30 lectins and antibodies against antigens of the abo-blood group system to find specific and sensitive markers for the basal cells of the human respiratory surface epithelium. three lectins always stained the basal cells: aaptos papillata agglutinin i (apa i), peanut agglutinin (pna), and wheat germ agglutinin (wga): other lectins and the antibodies gave positive results only in tissue of secretors (blood group antigens in secretions) and these were dependent on the abo-blo ... | 1997 | 9376654 |
| lectin histochemical hpa-binding pattern of human breast and colon cancers is associated with metastases formation in severe combined immunodeficient mice. | metastasis formation is a major clinical problem in cancer treatment, and no significant progress in the treatment of metastatic spread has been made. this apparent lack of progress is partly caused by the absence of clinically relevant animal models of metastases. the binding of the lectin helix pomatia agglutinin (hpa) has been associated with a poor prognosis in breast and colon cancer patients. hpa-positive and -negative human breast and colon cancer cell lines were transplanted into severe ... | 1997 | 9413741 |
| lectin binding sites on cd34+ human haematopoietic stem cells and lymphocytes from peripheral blood: an ultrastructural post-embedding study. | this study was performed to obtain a better insight into the glycosylation pattern of human cd34+ haematopoietic stem cells and lymphocytes from peripheral blood using an ultrastructural post-embedding technique. lectins applied were derived from canavalia ensiformis (con a), triticum vulgare (wga), lycopersicon esculentum (lea), limulus polyphemus (lpa), ulex europaeus-i (uea-i), bauhinia purpurea (bpa), glycine max (sba), helix pomatia (hpa), arachis hypogaea (pna) and erythrina cristagalli (e ... | 1997 | 9413743 |
| lectin histochemistry of the rat brain following thioacetamide-induced hepatic failure. | biotinyl derivatives of several lectins were used to study the localization of glycoconjugates in the cerebral microcapillaries and various brains of rats given at 24-h intervals two i.p. administrations of a hepatotoxin-thioacetamide (taa) and examined 21 d posttreatment. at this time, the rats were asymptomatic with regard to hepatic encephalopathy but showed specific and selective changes in the blood-brain-barrier (bbb) transport of basic amino acid, but no bbb damage, and region-specific ne ... | 1997 | 9437665 |
| developmental appearance and distribution of bone sialoprotein and osteopontin in human and rat cementum. | bone sialoprotein (bsp) and osteopontin (opn), two major noncollagenous proteins (ncps) in collagen-based mineralized tissues, have been implicated in mineral deposition and cell- and matrix-matrix interactions during root development. however, their role in cementogenesis is still a subject of debate. since distribution of proteins is indicative of function, we have analyzed their temporo-spatial appearance in relation to that of cementum collagen. | 1998 | 9458064 |
| do hpa and pha-l have the same binding pattern in metastasizing human breast and colon cancers? | glycoconjugates on the tumour cell surface are functionally important for the interaction of the tumour cell with its environment. several studies have demonstrated that particular carbohydrate residues on primary cancers are associated with metastasis. identification of such residues is possible using lectins, including helix pomatia agglutinin (hpa) which has a nominal monosaccharide specificity for n-acetyl galactosamine (galnac) and phaseolus vulgaris leucoagglutinin (pha-l) which recognizes ... | 1998 | 9461027 |
| lectin histochemical studies of mouse granulated metrial gland cells. | a lectin histochemical study has been carried out on mouse granulated metrial gland cells, the major leucocyte population that differentiates in the uterine wall in pregnancy. the binding characteristics of 26 lectins were examined using light microscopical methods. fourteen of the lectins, with affinities ranging through n-acetylgalactosamine, galactose, n-acetylglucosamine, mannose and sialic acid residues, bound to the cytoplasmic granules of granulated metrial gland cells, and each appeared ... | 1997 | 9466156 |
| invasion potential and n-acetylgalactosamine expression in a human melanoma model. | reactivity of the n-acetylgalactosamine-binding helix pomatia agglutinin (hpa) in tumours has been associated with poor prognosis and metastasis development. in our lox/femx-i human melanoma model, the binding of hpa correlates with experimental lung metastasis formation in athymic nude mice. in the present study, the metastatic potential of 2 human melanoma cell lines (lox and femx-i) was assessed in relation to carbohydrate and invasive phenotype. immunocytological and invasion assays highligh ... | 1998 | 9466664 |
| steroid sulphatase: expression, isolation and inhibition for active-site identification studies. | steroid sulphatase, which can hydrolyse 3-hydroxysteroid sulphates, has important roles in several physiological and pathological processes. a number of steroid sulphatase inhibitors have now been developed, of which the most potent to date is oestrone-3-o-sulphamate (emate). this inhibitor inactivates steroid sulphatase in an irreversible, time- and concentration-dependent manner. in order to be able to use a radiolabelled derivative of emate to study the active site, it will be essential to pr ... | 1998 | 9566745 |
| changes in localization of chosen lectins in gerbil's brain submitted to 3 and 4 minute-long cns ischemia. | the gerbils brains after 3- and 4-minute-long ischemia caused by bilateral common carotid artery occlusion and 14 days survival were investigated using lectin techniques. chosen lectins, represented by synthetic plant glycoproteids, which are specifically bound to particular sugar residues (receptors) located on the cell surfaces were examined. lectins recognizing the following sugar residues have been used in our experiment: 1. n-acetyl-d-galactosyl (using helix pomatia agg., hpa) 2. alpha-d-ma ... | 1998 | 9595860 |
| core alpha1,3-fucose is a key part of the epitope recognized by antibodies reacting against plant n-linked oligosaccharides and is present in a wide variety of plant extracts. | carbohydrates have been suggested to account for some ige cross-reactions between various plant, insect, and mollusk extracts, while some igg antibodies have been successfully raised against plant glycoproteins. a rat monoclonal antibody raised against elderberry abscission tissue (yz1/2.23) and rabbit polyclonal antiserum against horseradish peroxidase were screened for reactivity in enzyme-linked immunosorbent assay against a range of plant glycoproteins and extracts as well as neoglycoprotein ... | 1998 | 9621106 |
| scavestrogen sulfamates: correlation between estrone sulfatase inhibiting and antioxidant effects. | in the present study estrone sulfatase (steryl-sulfatase; ec 3.1.6.2) and phenylsulfatase (arylsulfatase b; ec 3.1.6.1) inhibiting as well as antioxidant effects exerted by ring b,c unsaturated sulfamates of estrone (j 1025), 17 beta-estradiol (j 1054, j 1059, j 1067), and 17 alpha-estradiol (j 1051, j 1064, j 1065) were examined as compared with their parent compounds, j 994, j 995, and j 1050, using six different in vitro models: (i) estrone sulfatase activity in human placental microsomes, (i ... | 1998 | 9635147 |
| cedia dau benzodiazepine screening assay: a reformulation. | the cedia dau benzodiazepine assay has been reformulated to include online hydrolysis of urinary benzodiazepine glucuronide conjugates. the new antibody possesses enhanced cross-reactivities toward the low-dose benzodiazepines, which are excreted at low urinary drug-metabolite concentrations. the screening method was evaluated using lorazepam as the probe benzodiazepine. four subjects each consumed a 1-mg lorazepam tablet. sequential urine voids over the same time intervals were collected for th ... | 2013 | 9681328 |
| screening and confirmatory determination of ractopamine residues in calves treated with growth promoting doses of the beta-agonist. | ractopamine (rct) is a phenethanolamine member of the family of beta-adrenergic agonists (beta-agonists). this class of compounds have become notable for their properties of enhancing the growth rates of farm animal species but are not licensed for use in europe. an elisa procedure employing a polyclonal antibody raised in a goat was developed to detect rct residues in bovine urine samples. the assay had a high sensitivity (calibration curve mid-point of 22 pg per well), allowing the analysis of ... | 1998 | 9709494 |
| assembly and architecture of invertebrate cytoplasmic intermediate filaments reconcile features of vertebrate cytoplasmic and nuclear lamin-type intermediate filaments. | the two major intermediate filament (if) proteins from the esophagus epithelium of the snail helix pomatia and the two major if proteins from muscle tissue of the nematode ascaris suum were investigated under a variety of assembly conditions. the lowest-order complexes from each of the four protostomic invertebrate (p-inv) if proteins are parallel, unstaggered dimers involving two-stranded alpha-helical coiled coil formation of their approximately 350 amino acid residue central rod domain (i.e. ... | 1998 | 9737925 |
| protostrongylid nematode infection of chamois (rupicapra rupicapra) at the bauges massif (french alps). | from 1979 to 1985, protostrongylid infections (small lungworms) of chamois were studied at the bauges hunting reserve, in the western alps. fifty-two chamois were necropsied on 24 sites and lung nematodes identified to species. from june to november 1985, a more detailed study was undertaken on four sites where chamois were the only ruminants. first-stage larvae excreted in feces and infection of snails intermediate hosts were monitored. the protostrongylid species were, arranged in decreasing o ... | 1998 | 9746285 |
| regulation of ocular mucin secretion by p2y2 nucleotide receptors in rabbit and human conjunctiva. | the effects of adenine analogues on secretion of high molecular weight, mucin-like glycoproteins (mucins) by conjunctival goblet cells were investigated using isolated rabbit and human conjunctiva. mucin secretion was assayed using a quantitative dot-blot assay of helix pomatia agglutinin-horseradish peroxidase binding to mucins absorbed to nitrocellulose filters. in rabbit conjunctiva, exogenous atp (10(-7)-10(-3) m) induced a concentration-dependent, four-fold increase in mucin secretion that ... | 1998 | 9778415 |
| detection of inflammation- and neoplasia-associated alterations in human large intestine using plant/invertebrate lectins, galectin-1 and neoglycoproteins. | commonly, plant and invertebrate lectins are accepted glycohistochemical tools for the analysis of normal and altered structures of glycans in histology and pathology. mammalian lectins and neoglycoproteins are recent additions to this panel for the detection of lectin-reactive carbohydrate epitopes and glycoligand-binding sites. the binding profiles of these three types of probes were comparatively analyzed in normal, inflamed and neoplastic large intestine. in normal colonic mucosa the intrace ... | 1998 | 9780361 |
| viral glycoproteins accumulate in newly formed annulate lamellae following infection of lymphoid cells by human herpesvirus 6. | ultrastructural analysis of hsb-2 t-lymphoid cells and human cord blood mononuclear cells infected with human herpesvirus 6 revealed the presence, in the cell cytoplasm, of annulate lamellae (al), which were absent in uninfected cells. time course analysis of the appearance of al following viral infection showed that no al were visible within the first 72 h postinfection and that their formation correlated with the expression of the late viral glycoprotein gp116. the requirement of active viral ... | 1998 | 9811708 |
| lectin histochemistry of dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats. | a lectin histochemical study was carried out on the dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats (htrs) and wistar rats (wrs) at 3, 7 and 24 weeks of age to clarify the lectinhistochemical characteristics of the skin during their development. the lectins examined were concanavalia ensiformis (con a), dolichos biflorus agglutinin (dba), griffonia simpliciolia (gs-i), helix pomatia agglutinin (hpa), arachis hypogaea (pna), glycine maximus agglutinin (sba), ulex europeus agglutinin ( ... | 1998 | 9816494 |
| novel forms of protein glycosylation. | a large number of new glycans, derived from glycoproteins, has been characterized in the past few years. o-linked fucose was found in epidermal growth factor-like domains of several proteins. for the n-linked glycans of helix pomatia hemocyanin, novel types of antennae were identified. the positions of noncarbohydrate substituents were established in n-glycans. c-mannosylation of a tryptophan residue was discovered in human ribonuclease 2 and is the first example of c-glycosylation in glycoprote ... | 1998 | 9818259 |
| altered anionic gbm components in monoclonal antibody against slit diaphragm-injected proteinuric rats. | we previously reported that monoclonal antibody (mab) 5-1-6 bound to renal filtration slits induces massive proteinuria without causing ultrastructural changes in the glomerulus. this study evaluated the underlying mechanisms of the increase in glomerular permeability. | 1998 | 9844125 |
| comparison of four immunoassays for the detection of lorazepam in urine. | four healthy patient subjects were each given a single, 1-mg lorazepam tablet. urine samples from all patient subjects were collected at 12 intervals (0-2, 2-5, 5-8, 8-11, 11-14, 14-24, 24-26, 26-29, 29-32, 32-35, 35-38, and 38-48 hours). an aliquot from each urine collection was screened using cloned enzyme donor immunoassay (cedia), enzyme-multiplied immunoassay technique (emit) ii, emit dau, and fluorescence polarization immunoassay (fpia) without and with hydrolysis using beta-glucuronidase. ... | 1998 | 9853986 |
| muc5ac mucin is a component of the human precorneal tear film. | mucins are important structural and functional components of the precorneal tear film, yet little is known of their composition and synthesis. the mrnas of muc1, muc4, and muc5ac have previously been identified in human conjunctiva. of these, only muc5ac mrna appears to be associated with goblet cells. the purpose of the this study was to quantify muc5ac transcript levels, to identify muc5ac protein in conjunctiva, tears, and goblet cells and to determine whether this mucin is secreted in respon ... | 1999 | 9888425 |
| innate immunity in insects: the role of multiple, endogenous serum lectins in the recognition of foreign invaders in the cockroach, blaberus discoidalis. | unlike vertebrates, insects do not have an ab-based nonself recognition system, and must rely totally on innate immunity to defend themselves from microbial invaders. the most likely candidates for recognizing foreign material in insects are the lectins, which have already been shown to be important in mammalian innate immunity. the hemolymph of the cockroach, blaberus discoidalis, contains multiple lectins, designated bdl1, bdl2, bdl3, and gsl (beta-1,3-glucan-specific lectin), two of which, na ... | 1999 | 9973417 |
| the use of the lectin helix pomatia agglutinin (hpa) as a prognostic indicator and as a tool in cancer research. | progress in treatment for cancer has enabled extension of the disease-free interval, and of the quality of life for patients, but there has been very little improvement in overall survival rates. the main reason for this has been the ineffectiveness of current therapies to kill all the cancer cells once they have spread to distant sites to form metastatic deposits. one marker which has proved to be useful in identifying those cancers which have the potential to spread is the lectin helix pomatia ... | 1999 | 9987666 |
| a two-component affinity chromatography purification of helix pomatia arylsulfatase by tyrosine vanadate. | the inhibition of helix pomatia arylsulfatase by the synergistic combination of n-acetyl-l-tyrosine ethyl ester and vanadate has been extended to affinity chromatography for purification. in the presence of vanadate, l-tyrosine ethyl ester (tee), immobilized on ch-sepharose 4b retained arylsulfatase from the digestive juice or lyophilized powder of h. pomatia. no enzyme was retained without vanadate or with arsenate or phosphate. arylsulfatase was eluted from the column matrix by removing the va ... | 1999 | 10049673 |
| glycoconjugate expression in follicle-associated epithelium (fae) covering the nasal-associated lymphoid tissue (nalt) in specific pathogen-free and conventional rats. | we examined lectin-histochemically the glycoconjugate expression in the follicle-associated epithelium (fae) covering the nasal-associated lymphoid tissue (nalt) in the rat under specific pathogen-free (spf) and conventional (cv) conditions and compared the results for spf and cv rats as well as for membranous (m) cells and adjacent ciliated respiratory epithelial (cre) cells in fae. n-acetylgalactosamine-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), glycine max (sba) and vicia ... | 1999 | 10067202 |