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Microbial community analysis in the termite gut and fungus comb of Odontotermes formosanus: the implication of Bacillus as mutualists.The microbial communities harbored in the gut and fungus comb of the fungus-growing termite Odontotermes formosanus were analyzed by both culture-dependent and culture-independent methods to better understand the community structure of their microflora. The microorganisms detected by denaturing gradient gel electrophoresis (DGGE), clonal selection, and culture-dependent methods were hypothesized to contribute to cellulose-hemicellulose hydrolysis, gut fermentation, nutrient production, the break ...201122092951
acid-base properties of a soil fungus, trichoderma harzianum. 199422165935
comparative study on the effect of chemicals on alternaria blight in indian mustard--a multi-location study in india.high severity of altemaria blight disease is a major constraint in production of rapeseed-mustard in india. the aim of this study was to investigate the suppressive potential of chemicals viz., zinc sulphate, borax, sulphur, potash and calcium sulphate, aqueous extracts viz., eucalyptus globosus (50 g l-1) leaf extract and garlic (allium sativum) bulb (20 g l-1) extract, cow urine and bio-agents trichoderma harzianum, pseudomonas fluorescence in comparison with the recommended chemical fungicide ...201122167952
Microbial consortium mediated reprogramming of defense network in pea to enhance tolerance against Sclerotinia sclerotiorum.Aims: To evaluate the potentiality of three rhizosphere microorganisms in suppression of Sclerotinia rot in pea in consortia mode and their impact on host defense responses. Methods and Results: Pseudomonas aeruginosa PJHU15, Trichoderma harzianum TNHU27 and Bacillus subtilis BHHU100 from rhizospheric soils were selected based on compatibility, antagonistic and plant growth promotion activities. The microbes were used as consortia to assess their ability to trigger the phenylpropanoid and anti ...201122181349
Rice straw-decomposing fungi and their cellulolytic and xylanolytic enzymes.Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cellulolytic and xylanolytic enzymes were assessed through a two-step process, wherein highly active cellulase- and/or hemicellulaseproducing fungi were selected in a first screening st ...201122210620
use of a new trichoderma harzianum strain isolated from the amazon rainforest with pretreated sugar cane bagasse for on-site cellulase production.the on-site production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. this study concerns the use of a specific cellulolytic enzyme complex for hydrolysis of pretreated sugar cane bagasse. glycosyl hydrolases (fpase, xylanase, and β-glucosidase) were produced using a new strain of trichoderma harzianum, isolated from the amazon rainforest and cultivated under different conditions. the influence of the carbon source was fi ...201222221990
arbuscular mycorrhizal protein mrna over-expression in bread wheat seedlings by trichoderma harzianum raifi (krl-ag2) elicitation.association between arbuscular mycorrhizal fungi (amf) and majority of terrestrial plant species provides many benefits to plants that range from stress alleviation and bioremediation in soils polluted with heavy metals to plant growth promotion and yield quantity. some non-arbuscular mycorrhizal fungi such as, trichoderma harzianum, are known to enhance the amf symbiosis with vascular plants. however, information about their role in amf symbiosis is still limited. shoots of (avocet s) wheat see ...201222227493
involvement of pachybasin and emodin in self-regulation of trichoderma harzianum mycoparasitic coiling.our aim was to determine the effects of two secondary metabolites secreted by trichoderma harzianum, pachybasin and emodin, on the mycoparasitic coiling behavior and camp content of t. harzianum. the number of t. harzianum coils around nylon 66 fiber was increased in the presence of r. solani. the number of t. harzianum coils around r. solani hyphae and nylon 66 fiber were significantly increased in the presence of pachybasin and emodin. the camp level in t. harzianum was significantly increased ...201222292460
preparation, characterisation and viability of encapsulated trichoderma harzianum upm40 in alginate-montmorillonite clay.microencapsulation is a process by which tiny parcels of an active ingredient are packaged within a second material for the purpose of shielding the active ingredient from the surrounding environment. this study aims to determine the ability of the microencapsulation technique to improve the viability of trichoderma harzianum upm40 originally isolated from healthy groundnut roots as effective biological control agents (bcas). alginate was used as the carrier for controlled release, and montmoril ...201222309479
expression, immobilization and enzymatic properties of glutamate decarboxylase fused to a cellulose-binding domain.escherichia coli-derived glutamate decarboxylase (gad), an enzyme that catalyzes the conversion of glutamic acid to gamma-aminobutyric acid (gaba), was fused to the cellulose-binding domain (cbd) and a linker of trichoderma harzianum endoglucanase ii. to prevent proteolysis of the fusion protein, the native linker was replaced with a s(3)n(10) peptide known to be completely resistant to e. coli endopeptidase. the cbd-gad expressed in e. coli was successfully immobilized on avicel, a crystalline ...201222312257
transcriptomic response of arabidopsis thaliana after 24 h incubation with the biocontrol fungus trichoderma harzianum.trichoderma harzianum is a fungus used as biocontrol agent using its antagonistic abilities against phytopathogenic fungi, although it has also direct effects on plants, increasing or accelerating their growth and resistance to diseases and the tolerance to abiotic stresses. we analyzed arabidopsis thaliana gene expression changes after 24 h of incubation in the presence of t. harzianum t34 using the affymetrix genechip arabidopsis ath1. because this microarray contains more than 22,500 probe se ...201222317785
effect of arbuscular mycorrhizal (am) fungus and plant growth promoting rhizomicroorganisms (pgpr's) on medicinal plant solanum viarum seedlings.a green house nursery study was conducted to assess the interaction between arbuscular mycorrhizal (am) fungus, glomus aggregatum and some plant growth promoting rhizomicrooganisms (pgpr's), bacillus coagulans and trichoderma harzianum, in soil and their consequent effect on growth, nutrition and content of secondary metabolities of solanum viarum seedlings. triple inoculation of g. aggregatum+b. coagulans+t. harzainum with solanum viarum in a green house nursery study resulted in maximum plant ...201122319872
monomeric l-amino acid oxidase-induced mitochondrial dysfunction in rhizoctonia solani reveals a novel antagonistic mechanism of trichoderma harzianum ets 323.the monomeric l-amino acid oxidase (mth-laao) of trichoderma harzianum ets 323 has been suggested to antagonize rhizoctonia solani by an unknown mechanism. here, the mth-laao-treated r. solani exhibited hyphal lysis and apoptotic characteristics such as dna fragmentation, reactive oxygen species (ros) accumulation, lipid peroxidation, and mitochondrial membrane potential depolarization. this hyphal lysis was suppressed by the mitochondria-dependent apoptosis inhibitor oligomycin while accompanie ...201222352318
identification and characterization of the trichoderma harzianum gene encoding alpha-1,3-glucanase involved in streptococcal mutan degradation.alpha-1,3-glucanases (mutanases) are currently of great interest due to their potential use in the field of dental care. these enzymes have been reported in several bacteria, yeasts and fungi, but up to now, characterization of this family of proteins has been relatively poor. in this study, we identify and characterize a mutanase gene from trichoderma harzianum ccm f-340. sequence analysis, on the nucleotide and amino acid levels reveals that this alpha-1,3-glucanase is highly homologous to alp ...201122390063
biochemical and physiological responses of rice (oryza sativa l.) as influenced by trichoderma harzianum under drought stress.rice is one of the most important food crops drastically affected by drought in lowland rice ecosystems. in the present study, the impact of drought tolerant isolates of endophytic fungus trichoderma harzianum on rice response to drought was investigated. out of 43 trichoderma isolates studied, only five isolates viz., th 56, th 69, th 75, th 82 and th 89 were selected to be drought tolerant as these were able to colonize well on cow dung at low moisture content of 10-20 percent, though two isol ...201222391125
expression of genes of trichoderma harzianum in response to the presence of cadmium in the substrate.trichoderma harzianum is a very important fungus for the reduction of the amount of heavy metals resulting from agricultural and industrial activities. this filamentous fungus has been shown to be tolerant towards several heavy metals (e.g. cd, pb, zn, ni and mn), but the mechanism underlying this tolerance is not entirely understood. in this study, we confirmed the ability of t. harzianum to grow in the presence of cadmium and observed a significant stimulation of its growth in the presence of ...201222400914
separation and identification of volatile compounds from liquid cultures of trichoderma harzianum by gc-ms using three different capillary columns.a simple, fast, repeatable and less laborious sample preparation protocol was developed and applied for the analysis of biocontrol fungus trichoderma harzianum strain fa1132 by using gas chromatography-mass spectrometry. the match factors for sample spectra with respect to the mass spectra library of fungal volatile compounds were determined and used to study the complex hydrocarbons and other volatile compounds, which were separated by using different capillary columns with nonpolar, medium pol ...201222407347
crystallization and preliminary x-ray diffraction analysis of endoglucanase iii from trichoderma harzianum.endoglucanases are enzymes that hydrolyze cellulose and are important components of the cellulolytic complex. in contrast to other members of the complex, they cleave internal β-1,4-glycosidic bonds in the cellulose polymer, allowing cellulose to be used as an energy source. since biomass is an important renewable source of energy, the structural and functional characterization of these enzymes is of interest. in this study, endoglucanase iii from trichoderma harzianum was produced in pichia pas ...201222442229
qpcr quantification of sphaerodes mycoparasitica biotrophic mycoparasite interaction with fusarium graminearum: in vitro and in planta assays.sphaerodes mycoparasitica, a biotrophic mycoparasite of fusarium species, improved wheat seed germination and seedling growth in vitro compared to trichoderma harzianum, a necrotrophic mycoparasite. however, under phytotron conditions, both s. mycoparasitica and t. harzianum had positive impact on wheat seedlings growth in the presence of f. graminearum. once exposed to the mycoparasites, the dna quantity of f. graminearum in wheat root decreased. observed shifts in dna quantity using qpcr, a se ...201222476566
trichoderma harzianum enhances antioxidant defense of tomato seedlings and resistance to water deficit.some plant-symbiotic strains of the genus trichoderma colonize roots and induce profound changes in plant gene expression that lead to enhanced growth, especially under biotic and abiotic stresses. in this study, we tested the hypothesis that one of the protective mechanisms enhanced by t. harzianum t22 colonization is the antioxidant defense mechanism. having established that strain t22 modulates the expression of the genes encoding antioxidant enzymes, the status of antioxidant defense of toma ...201222512380
antifungal saponins from bulbs of garlic, allium sativum l. var. voghiera.a bioassay-guided phytochemical analysis of the polar extract from the bulbs of garlic, allium sativum l., var. voghiera, typical of voghiera, ferrara (italy), allowed the isolation of ten furostanol saponins; voghieroside a1/a2 and voghieroside b1/b2, based on the rare agapanthagenin aglycone; voghieroside c1/c2, based on agigenin aglycone; and voghieroside d1/d2 and e1/e2, based on gitogenin aglycone. in addition, we found two known spirostanol saponins, agigenin 3-o-trisaccharide and gitogeni ...201222513009
application of trichoderma harzianum sqr-t037 bio-organic fertiliser significantly controls fusarium wilt and affects the microbial communities of continuously cropped soil of cucumber.the reduction in diversity of the soil microbial community causes the disorder of continuous cropping. the aim of this study was to determine the effects of applying trichoderma harzianum sqr-t037 bio-organic fertiliser (bio) on the microbial community in continuously cropped cucumber soil. four treatments were set: (1) control, where neither seedling nursery soil (n) nor transplanted soil (t) was amended with bio; (2) n treatment, where nursery soil was amended with bio (1% w/w) but transplante ...201222513876
single-cell protein as an alternative food for zebrafish, danio rerio: a toxicological assessment.single-cell protein (scp) refers to the dried cells of microorganisms. the aim of this research was to evaluate the nutrional characteristics and possible toxic effects of the scp of trichoderma harzianum. first, t. harzianum was grown on whey filtrate agar medium and the obtained scp was analysed. it was rich in both total protein (34.21%) and ash (4.78%). furthermore, the biomass contained all the essential amino acids, and the amino acid concentrations were very close to the fao reference pro ...201322514118
antagonistic potentiality of trichoderma harzianum towards seed-borne fungal pathogens of winter wheat cv. protiva in vitro and in vivo.the antagonistic effect of trichoderma harzianum on a range of seed-borne fungal pathogens of wheat (viz. fusarium graminearum, bipolaris sorokiniana, aspergillus spp., and penicillium spp.) was assessed. the potential of t. harzianum as a biocontrol agent was tested in vitro and under field conditions. coculture of the pathogens and trichoderma under laboratory conditions clearly showed dominance of t. harzianum. under natural conditions, biocontrol effects were also obtained against the test f ...201222561850
integrated management of foot rot of lentil using biocontrol agents under field condition.the efficacy of cowdung, bangladesh institute of nuclear agriculture (bina)-biofertilizer, and bangladesh agricultural university (bau)-biofungicide, alone or in combination, was evaluated for controlling foot rot disease of lentil. the results exhibited that bina-biofertilizer and baubiofungicide (peat soil-based rhizobium leguminosarum and black gram bran-based trichoderma harzianum) are compatible and have combined effects in controlling the pathogenic fungi fusarium oxysporum and sclerotium ...201222580305
[investigation of antitumor substance from trichoderma].the effect of l-lysine-alpha-oxidase from trichoderma harzianum rifai on the functional activity of t-lymphocytes was investigated. it was shown that in a dose of 35 units/kg administered parentally the enzyme had no suppressive effect on the t-lymphocyte functional activity. an inhibitory effect of l-lysine-a-oxidase on some indices of the macrophages functional activity was observed. l-lyzine-alpha-oxidase had a selective lymphotropic action and showed no mytostatic activity, which is in favou ...201122586896
a cellulolytic hypocrea strain isolated from south american brave straw produces a modular xylanase.cellulase-producing fungi from the andean regions in bolivia, an ecosystem characterized as an extreme arid highland, were studied. thirty-two isolates were screened for presence of cellulase activity using carboxymethyl cellulose (cmc) as carbon source, and activity was confirmed using a filter paper assay. one isolate, denoted as blt1c was selected from this screening, and sequence analysis of the internal transcribed spacer (its) classified the strain as hypocrea lixii. the secretome of blt1c ...201222609439
recombinant expression and characterization of an endoglucanase iii (cel12a) from trichoderma harzianum (hypocreaceae) in the yeast pichia pastoris.filamentous fungi from the genus trichoderma have been widely investigated due to their considerable production of important biotechnological enzymes. previous studies have demonstrated that the t. harzianum strain ioc-3844 has a high degree of cellulolytic activity. after excluding the native signal peptide, the open reading frame of the t. harzianum endoglucanase iii enzyme was cloned in the expression vector ppiczαa, enabling protein secretion to the culture medium. the recombinant plasmid wa ...201222653604
isolation and functional analysis of thmfs1, the first major facilitator superfamily transporter from the biocontrol fungus trichoderma harzianum.a novel major facilitator superfamily (mfs) transporter gene, thmfs1, was isolated from trichoderma harzianum (t. harzianum). a thmfs1 over-expressing mutant displayed enhanced antifungal activity and fungicide tolerance, while the thmfs1 disruption mutant showed the opposite trend. trichodermin production in thmfs1 disruption group (185 mg l(-1)) was decreased by less than 17 % compared to the parental strain, suggesting that thmfs1 is not mainly responsible for trichodermin secretion. real-tim ...201222661043
antagonism of trichoderma harzianum ets 323 on botrytis cinerea mycelium in culture conditions.abstract previous studies have shown that the extracellular proteins of trichoderma harzianum ets 323 grown in the presence of deactivated botrytis cinerea in culture include a putative l-amino acid oxidase and have suggested the involvement of this enzyme in the antagonistic mechanism. here, we hypothesized that the mycoparasitic process of trichoderma spp. against b. cinerea involves two steps; that is, an initial hyphal coiling stage and a subsequent hyphal coiling stage, with different coili ...201222734558
blue native-page analysis of trichoderma harzianum secretome reveals cellulases and hemicellulases working as multienzymatic complexes.plant cell wall-degrading enzymes produced by microorganisms possess important biotechnological applications, including biofuel production. some anaerobic bacteria are able to produce multienzymatic complexes called cellulosomes while filamentous fungi normally secrete individual hydrolytic enzymes that act synergistically for polysaccharide degradation. here, we present evidence that the fungus trichoderma harzianum, cultivated in medium containing the agricultural residue sugarcane bagasse, is ...201222744980
secretome analysis of the fungus trichoderma harzianum grown on cellulose.trichoderma harzianum is a mycoparasitic filamentous fungus that produces and secretes a wide range of extracellular hydrolytic enzymes used in cell wall degradation. due to its potential in biomass conversion, t. harzianum draws great attention from biofuel and biocontrol industries and research. here, we report an extensive secretome analysis of t. harzianum. the fungus was grown on cellulose medium, and its secretome was analyzed by a combination of enzymology, 2de, maldi-ms and -ms/ms (autof ...201222745025
biochemical and metabolic profiles of trichoderma strains isolated from common bean crops in the brazilian cerrado, and potential antagonism against sclerotinia sclerotiorum.some species of trichoderma have successfully been used in the commercial biological control of fungal pathogens, e.g., sclerotinia sclerotiorum, an economically important pathogen of common beans (phaseolus vulgaris l.). the objectives of the present study were (1) to provide molecular characterization of trichoderma strains isolated from the brazilian cerrado; (2) to assess the metabolic profile of each strain by means of biolog ff microplates; and (3) to evaluate the ability of each strain to ...201222749168
identification of differentially expressed genes in response to mercury i and ii stress in trichoderma harzianum.filamentous fungi are very promising organisms in both the control and the reduction of the amount of heavy metal released by human and industrial activities. in particular, trichoderma harzianum demonstrated to be tolerant towards different heavy metals, such as mercury and cadmium, even though the mechanism underlying this tolerance is not fully understood. by using a particular strategy of the suppression subtractive hybridization technique, we were able to identify in the strain imi 393899 o ...201222789863
technology for efficient and successful delivery of vermicompost colonized bioinoculants in pogostemon cablin (patchouli) benth.the usefulness of vermicompost as a supporting media for growth of bioinoculants was evaluated for successful transfer of sufficient propagules of bioinoculants into the organic fields. the rooted plants after 50 days were pot and field tested for their growth and yield performances when transplanted along with rooting medium into pots/organic fields. the rooting medium, 50 days of inoculation, contained sufficient population of bioinoculants and arbuscular mycorrhizal (am) fungi. treatment with ...201222806808
differential display of abundantly expressed genes of trichoderma harzianum during colonization of tomato-germinating seeds and roots.the identification of trichoderma genes whose expression is altered during early stages of interaction with developing roots of germinated seeds is an important step toward understanding the rhizosphere competency of trichoderma spp. the potential of 13 trichoderma strains to colonize tomato root and promote plant growth has been evaluated. all used strains successfully propagated in spermosphere and continued their growth in rhizoplane simultaneously root enlargement while the strains t6 and t7 ...201222810958
sequence analysis and gene expression of putative exo- and endo-glucanases from oil palm (elaeis guineensis) during fungal infection.glucanases are enzymes that hydrolyze a variety β-d-glucosidic linkages. plant β-1,3-glucanases are able to degrade fungal cell walls; and promote the release of cell-wall derived fungal elicitors. in this study, three full-length cdna sequences encoding oil palm (elaeis guineensis) glucanases were analyzed. sequence analyses of the cdna sequences suggested that egglc1-1 is a putative β-d-glucan exohydolase belonging to glycosyl hydrolase (gh) family 3 while egglc5-1 and egglc5-2 are putative gl ...201222854183
a novel method to prepare concentrated conidial biomass formulation of trichoderma harzianum for seed application.to prepare concentrated formulation of trichoderma harzianum mtcc-3841 (nbri-1055) with high colony forming units (cfu), long shelf life and efficient in root colonization by a simple scrapping method.201222897244
mrna expression of egchi1, egchi2, and egchi3 in oil palm leaves (elaeis guineesis jacq.) after treatment with ganoderma boninense pat. and trichoderma harzianum rifai.basal stem rot (bsr) disease caused by the fungus ganoderma boninense is the most serious disease affecting the oil palm; this is because the disease escapes the early disease detection. the biocontrol agent trichoderma harzianum can protect the disease only at the early stage of the disease. in the present study, the expression levels of three oil palm (elaeis guineensis jacq.) chitinases encoding egchi1, egchi2, and egchi3 at 2, 5, and 8 weeks inoculation were measured in oil palm leaves from ...201222919345
comparative studies on the induction of trichoderma harzianum mutanase by α-(1→3)-glucan-rich fruiting bodies and mycelia of laetiporus sulphureus.mutanase (α-(1→3)-glucanase) is a little-known inductive enzyme that is potentially useful in dentistry. here, it was shown that the cell wall preparation (cwp) obtained from the fruiting body or vegetative mycelium of polypore fungus laetiporus sulphureus is rich in α-(1→3)-glucan and can be successfully used for mutanase induction in trichoderma harzianum. the content of this biopolymer in the cwp depended on the age of fruiting bodies and increased along with their maturation. in the case of ...201222949817
fusant trichoderma hf9 with enhanced extracellular chitinase and protein content.strain improvement was carried out to obtain higher chitinase and protein by inter-specific protoplast fusion between trichoderma harzianum and trichoderma viride. fusant hf9 and parental strains of trichoderma were compared for chitinase and protein production. 1% of glucose, sucrose and fungal cell wall (rhizoctonia solani), were used as carbon source for cultivation of trichoderma and fungal cell wall was the best to induce chitinase and protein. usage of 0.5% colloidal chitin for the fungal ...201623035579
trichoderma harzianum ets 323-mediated resistance in brassica oleracea var. capitata to rhizoctonia solani involves the novel expression of a glutathione s-transferase and a deoxycytidine deaminase.plant interactions with microbial biocontrol agents are used as experimental models to understand resistance-related molecular adaptations of plants. in a hydroponic three-way interaction study, a novel trichoderma harzianum ets 323 mediated mechanism was found to induce resistance to rhizoctonia solani infection in brassica oleracea var. capitata plantlets. the r. solani challenge on leaves initiate an increase in lipoxygenase activity and associated hypersensitive tissue damage with characteri ...201223046447
structural diversity of streptococcal mutans synthesized under different culture and environmental conditions and its effect on mutanase synthesis.streptococcal mutans synthesized under different conditions by growing cultures or by their glucosyltransferases were shown to exhibit a great structural and property diversity. culturing and environmental factors causing structural differences in mutans were specified. all of the obtained biopolymers (76 samples) were water-insoluble and most of them (72) had a structure with a predominance of α-(1→3)-linked glucose (i.e., the content of α-(1→3)-linkages in the glucan was always higher than 50% ...201223047481
isotope-assisted screening for iron-containing metabolites reveals a high degree of diversity among known and unknown siderophores produced by trichoderma spp.due to low iron availability under environmental conditions, many microorganisms excrete iron-chelating agents (siderophores) to cover their iron demands. a novel screening approach for the detection of siderophores using liquid chromatography coupled to high-resolution tandem mass spectrometry was developed to study the production of extracellular siderophores of 10 wild-type trichoderma strains. for annotation of siderophores, an in-house library comprising 422 known microbial siderophores was ...201323064341
sequence analysis and gene expression of putative oil palm chitinase and chitinase-like proteins in response to colonization of ganoderma boninense and trichoderma harzianum.chitinases are glycosyl hydrolases that cleave the β-1,4-glycosidic linkages between n-acetylglucosamine residues in chitin which is a major component of fungal cell wall. plant chitinases hydrolyze fungal chitin to chitin oligosaccharides that serve as elicitors of plant defense system against fungal pathogens. however, plants synthesize many chitinase isozymes and some of them are not pathogenesis-related. in this study, three full-length cdna sequences encoding a putative chitinase (egchit3-1 ...201323065213
proteomic analysis of grapevine resistance induced by trichoderma harzianum t39 reveals specific defence pathways activated against downy mildew.downy mildew is caused by the oomycete plasmopara viticola and is one of the most serious diseases of grapevine. the beneficial microorganism trichoderma harzianum t39 (t39) has previously been shown to induce plant-mediated resistance and to reduce the severity of downy mildew in susceptible grapevines. in order to better understand the cellular processes associated with t39-induced resistance, the proteomic and histochemical changes activated by t39 in grapevine were investigated before and 1 ...201223105132
joint x-ray crystallographic and molecular dynamics study of cellobiohydrolase i from trichoderma harzianum: deciphering the structural features of cellobiohydrolase catalytic activity.aiming to contribute toward the characterization of new, biotechnologically relevant cellulolytic enzymes, we report here the first crystal structure of the catalytic core domain of cel7a (cellobiohydrolase i) from the filamentous fungus trichoderma harzianum ioc 3844. our structural studies and molecular dynamics simulations show that the flexibility of tyr260, in comparison with tyr247 from the homologous trichoderma reesei cel7a, is enhanced as a result of the short side-chains of adjacent va ...201323114223
downy mildew resistance induced by trichoderma harzianum t39 in susceptible grapevines partially mimics transcriptional changes of resistant genotypes.downy mildew, caused by plasmopara viticola, is one of the most severe diseases of grapevine and is commonly controlled by fungicide treatments. the beneficial microorganism trichoderma harzianum t39 (t39) can induce resistance to downy mildew, although the molecular events associated with this process have not yet been elucidated in grapevine. a next generation rna sequencing (rna-seq) approach was used to study global transcriptional changes associated with resistance induced by t39 in vitis v ...201223173562
anti-trichomonas vaginalis activity of marine-associated fungi from the south brazilian coast.trichomonas vaginalis is the causative agent of trichomonosis, the most common non-viral sexually transmitted disease. infection with this protozoan may have serious consequences, especially for women. currently, 5-nitroimidazole drugs are the treatment of choice for trichomonosis, but the emergence of resistance has limited the effectiveness of this therapy. in this context, this study aimed to evaluate the anti-t. vaginalis activity of marine-associated fungi found in the south brazilian coast ...201323201217
[isolation, identification and enzymological characterization of a new fungal with high laccase production from agricultural waste composting].a new fungal strain with high laccase production was isolated from the agricultural waste composting. it was identified as hypocrea lixii based on morphological analysis and its sequence. the partial laccase gene of the new strain was successfully amplified using the primer pair cu1af and cu2r. after purification and cloning of the pcr product, a 148 bp fragment of laccase gene was obtained. the laccase activity of the strain reached a peak value of 67.5 u x ml(-1) in liquid medium. the molecula ...201223243884
wetting properties of fungi mycelium alter soil infiltration and soil water repellency in a γ-sterilized wettable and repellent soil.soil water repellency (swr) has a drastic impact on soil quality resulting in reduced infiltration, increased runoff, increased leaching, reduced plant growth, and increased soil erosion. one of the causes of swr is hydrophobic fungal structures and exudates that change the soil-water relationship. the objective of this study was to determine whether swr and infiltration could be manipulated through inoculation with fungi. the effect of fungi on swr was investigated through inoculation of three ...201223245615
experimental mixture design as a tool to enhance glycosyl hydrolases production by a new trichoderma harzianum p49p11 strain cultivated under controlled bioreactor submerged fermentation.this work investigates the glycosyl hydrolase (gh) profile of a new trichoderma harzianum strain cultivated under controlled bioreactor submerged fermentation. the influence of different medium components (delignified steam-exploded sugarcane bagasse, sucrose, and soybean flour) on gh biosynthesis was assessed using experimental mixture design (emd). additionally, the effect of increased component concentrations in culture media selected from the emd was studied. it was found that that a mixed c ...201323265822
in vitro and in vivo biocontrol of soil-borne phytopathogenic fungi by certain bioagents and their possible mode of action.the study aimed to investigate new wide-spectrum biological control agents against soil-borne fungal plant pathogens. screening of 336 fungal strains and 256 bacterial strains was carried out in vitro to select the most promising isolates for controlling destructive pathogens of greenhouse-vegetables. in dual cultures, bacillus subtilis jf419701 and trichoderma harzianum jf419706 (teleomorph: hypocrea lixii) inhibited the growth of alternaria alternata, fusarium oxysporum, exserohilum rostratum, ...201223269217
anticancer and antimicrobial activity of mangrove derived fungi hypocrea lixii vb1.mangrove is one of the oldest living tree species and its leaves are among the most extensively studied botanicals in use today. scientific research throughout the world has found evidence to support the fact that its foliar extracts have great potential against human microbial pathogens. this study highlights the isolation of foliar fungi from rhizophora mucronata, avicenna officialis and avicenna marina.201223302537
genetic diversity and species pattern of trichoderma and hypocrea in manipur using in silico analysis.we investigated the occurrence and genetic diversity of trichoderma and hypocrea in manipur which lies in the indo-burma biodiversity hot spot region. 65 trichoderma isolates were identified at species level by morphological as well as sequence based analysis of the internal transcribed spacer region 1 and 4. altogether 22 different species of trichoderma and hypocrea were found, of which trichoderma harzianum represent the dominant species. phylogenetic analysis reveals a clear cut distinction ...201323390355
understanding the cellulolytic system of trichoderma harzianum p49p11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectinase and α-l-arabinofuranosidase.supplementation of cellulase cocktails with accessory enzymes can contribute to a higher hydrolytic capacity in releasing fermentable sugars from plant biomass. this study investigated which enzymes were complementary to the enzyme set of trichoderma harzianum in the degradation of sugarcane bagasse. specific activities of t. harzianum extract on different substrates were compared with the extracts of penicillium echinulatum and trichoderma reesei, and two commercial cellulase preparations. comp ...201323391738
hypocrea lixii, novel endophytic fungi producing anticancer agent cajanol, isolated from pigeon pea (cajanus cajan [l.] millsp.).the aim was to isolate, identify and characterize endophytes from pigeon pea (cajanus cajan [l.] millsp.), as novel producer of cajanol and its in vitro cytotoxicity assay.201323495919
identification of differentially expressed genes from trichoderma harzianum during growth on cell wall of fusarium solani as a tool for biotechnological application.the species of t. harzianum are well known for their biocontrol activity against many plant pathogens. however, there is a lack of studies concerning its use as a biological control agent against f. solani, a pathogen involved in several crop diseases. in this study, we have used subtractive library hybridization (ssh) and quantitative real-time pcr (rt-qpcr) techniques in order to explore changes in t. harzianum genes expression during growth on cell wall of f. solani (fscw) or glucose. rt-qpcr ...201323497274
x-ray structure and molecular dynamics simulations of endoglucanase 3 from trichoderma harzianum: structural organization and substrate recognition by endoglucanases that lack cellulose binding module.plant biomass holds a promise for the production of second-generation ethanol via enzymatic hydrolysis, but its utilization as a biofuel resource is currently limited to a large extent by the cost and low efficiency of the cellulolytic enzymes. considerable efforts have been dedicated to elucidate the mechanisms of the enzymatic process. it is well known that most cellulases possess a catalytic core domain and a carbohydrate binding module (cbm), without which the enzymatic activity can be drast ...201323516599
chemical composition and antifungal activity of matricaria recutita flower essential oil against medically important dermatophytes and soil-borne pathogens.fungal infections are potential public health threats all over the world. in the present study, effect of matricaria recutita flower essential oil (eo) was evaluated against medically important dermatophytes and opportunistic saprophytes using microbioassay technique.201223518164
purification and properties of an α-(1 → 3)-glucanase (ec 3.2.1.84) from trichoderma harzianum and its use for reduction of artificial dental plaque accumulation.extracellular α-(1 → 3)-glucanase (mutanase, ec 3.2.1.84) produced by trichoderma harzianum ccm f-340 was purified to homogeneity by ultrafiltration followed by ion exchange and hydrophobic interaction chromatography, and final chromatofocusing. the enzyme was recovered with an 18.4-fold increase in specific activity and a yield of 4.3%. some properties of the α-(1 → 3)-glucanase were investigated. the molecular mass of the enzyme is 67 kda, as estimated by sds/page, its isoelectric point 7.1, a ...201323520578
effect of fungi and light on seed germination of three opuntia species from semiarid lands of central mexico.fungal attack under light reduces mechanical resistance of the testa of opuntia seeds, making it easier for the embryo to emerge. however, the effect of fungi on opuntia seed germination in darkness is unknown. we evaluated the combined effects of light and inoculation with phoma medicaginis, trichoderma harzianum, trichoderma koningii, and penicillium chrysogenum on germination of o. streptacantha, o. leucotricha, and o. robusta seeds, from central mexico. we also evaluated the combined effects ...201323526153
water-soluble (1→3),(1→4)-α-d-glucan from mango as a novel inducer of cariogenic biofilm-degrading enzyme.a water-soluble polysaccharide (wsp) extracted from mango (mangifera indica l.) fruits has been suggested as a new alternative to mutan for mutanase induction in trichoderma harzianum. structural analyses revealed that the purified wsp was a (1→3),(1→4)-α-d-glucan with the molecular mass of ca. 760 kda in which the (1→4)-linked and (1→3)-linked α-glcp residues were in a ratio of 1:2.4. when the strain t harzianum ccm f-340 was grown in the presence of wsp, the maximal enzyme productivity obtaine ...201323583288
influence of the fungus trichoderma harzianum on the enzyme and polysaccharide composition of silene vulgaris callus.activities of polygalacturonase and 1,3-β-glucanase increased in campion (silene vulgaris) callus cells during co-cultivation with the fungus trichoderma harzianum. this was associated with a decrease in galacturonic acid residues in the pectic polysaccharide of campion silenan and also in the production of pectin by the callus. co-cultivation of the callus and the fungus resulted in an increase in contents of arabinose residues in the intracellular arabinogalactan and in contents of galactose r ...201323586723
fast methods for screening of trichothecenes in fungal cultures using gc-ms/ms.fast methods for screening for production of simple and macrocyclic trichothecenes, produced on solid substrates were developed. crude extracts fromfusarium cultures grown on yeast extract sucrose agar (approx. 0.3 cm(2) culture) were derivatised using pentafluoropropionic anhydride and analysed by gc - tandem mass spectrometry using a finnigan gcq(+) ion trap. the ms was operated in the ei(+) multiscan mode allowing simultaneous full scan and ms/ms of 3-4 parent ions. production of acetyl t-2 t ...200023605545
construction of a streptomyces lydicus a01 transformant with a chit42 gene from trichoderma harzianum p1 and evaluation of its biocontrol activity against botrytis cinerea.streptomyces lydicus a01 and trichoderma harzianum p1 are potential biocontrol agents of fungal diseases in plants. s. lydicus a01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of botrytis cinerea. t. harzianum p1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of b. cinerea. to obtain the synergistic biocontrol effects of chitinase and natamycin on botrytis cinerea, this study transformed the chit42 ...201323625216
cloning, annotation and expression analysis of mycoparasitism-related genes in trichoderma harzianum 88.trichoderma harzianum 88, a filamentous soil fungus, is an effective biocontrol agent against several plant pathogens. high-throughput sequencing was used here to study the mycoparasitism mechanisms of t. harzianum 88. plate confrontation tests of t. harzianum 88 against plant pathogens were conducted, and a cdna library was constructed from t. harzianum 88 mycelia in the presence of plant pathogen cell walls. randomly selected transcripts from the cdna library were compared with eukaryotic plan ...201323625217
mycoparasitism studies of trichoderma species against three phytopathogenic fungi: evaluation of antagonism and hydrolytic enzyme production.trichoderma spp. are used for biocontrol of several plant pathogens. however, their efficient interaction with the host needs to be accompanied by production of secondary metabolites and cell wall-degrading enzymes. three parameters were evaluated after interaction between four trichoderma species and plant-pathogenic fungi: fusarium solani, rhizoctonia solani and sclerotinia sclerotiorum. trichoderma harzianum and t. asperellum were the most effective antagonists against the pathogens. most of ...201323690037
the effect of iron and copper as an essential nutrient on mitochondrial electron transport system and lipid peroxidation in trichoderma harzianum.iron and copper are essential nutrients for all living organisms as cofactors of many enzymes and play important roles in electron transport system (ets) enzymes which have heme and iron-sulfur centers. in the present study, ets enzymes, namely, succinate dehydrogenase (sdh) and cytochrome c oxidase (cox), activities as well as adenine nucleotides and lipid peroxidation (lpo) levels of eukaryotic model trichoderma harzianum grown in varied concentrations of iron (0-20 mg/l) and copper (0-25 mg/l ...201323716140
antifungal activity and cytotoxicity of isolated compounds from leaves of breonadia salicina.breonadia salicina is used traditionally to treat wounds, ulcers, fevers, headaches, and fungal infections. the aim of this study was to investigate the antifungal activity of the plant extract and compounds isolated there from.201323727185
expression profiles of putative defence-related proteins in oil palm (elaeis guineensis) colonized by ganoderma boninense.basal stem rot (bsr) is a major disease of oil palm caused by a pathogenic fungus, ganoderma boninense. however, the interaction between the host plant and its pathogen is not well characterized. to better understand the response of oil palm to g. boninense, transcript profiles of eleven putative defence-related genes from oil palm were measured by quantitative reverse-transcription (qrt)-pcr in the roots of oil palms treated with g. boninense from 3 to 12 weeks post infection (wpi). these trans ...201323769496
spirostane, furostane and cholestane saponins from persian leek with antifungal activity.a phytochemical investigation of the seeds of persian leek afforded the isolation of two new spirostane glycosides, persicosides a (1) and b (2), four new furostane glycosides, isolated as a couple of inseparable mixture, persicosides c1/c2 (3a/3b) and d1/d2 (4a/4b), one cholestane glycoside, persicoside e (5), together with the furostane glycosides ceposides a1/a2 and c1/c2 (6a/6b and 7a/7b), tropeosides a1/a2 and b1/b2 (8a/8b and 9a/9b), and ascalonicoside a1/a2 (10a/10b), already described in ...201323790946
deciphering the hormonal signalling network behind the systemic resistance induced by trichoderma harzianum in tomato.root colonization by selected trichoderma isolates can activate in the plant a systemic defense response that is effective against a broad-spectrum of plant pathogens. diverse plant hormones play pivotal roles in the regulation of the defense signaling network that leads to the induction of systemic resistance triggered by beneficial organisms [induced systemic resistance (isr)]. among them, jasmonic acid (ja) and ethylene (et) signaling pathways are generally essential for isr. however, trichod ...201323805146
abiotic stresses affect trichoderma harzianum t39-induced resistance to downy mildew in grapevine.enhancement of plant defense through the application of resistance inducers seems a promising alternative to chemical fungicides for controlling crop diseases but the efficacy can be affected by abiotic factors in the field. plants respond to abiotic stresses with hormonal signals that may interfere with the mechanisms of induced systemic resistance (isr) to pathogens. in this study, we exposed grapevines to heat, drought, or both to investigate the effects of abiotic stresses on grapevine resis ...201323841621
dna barcoding survey of trichoderma diversity in soil and litter of the colombian lowland amazonian rainforest reveals trichoderma strigosellum sp. nov. and other species.the diversity of trichoderma (hypocreales, ascomycota) colonizing leaf litter as well as the rhizosphere of garcinia macrophylla (clusiaceae) was investigated in primary and secondary rain forests in colombian amazonia. dna barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (its1 and 2) of the ribosomal rna gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of trichoderma was dominated (71 %) by three ...201323884864
harzianic acid: a novel siderophore from trichoderma harzianum.agriculture-relevant microorganisms are considered to produce secondary metabolites during processes of competition with other micro- and macro-organisms, symbiosis, parasitism or pathogenesis. many different strains of the genus trichoderma, in addition to a direct activity against phytopathogens, are well-known producers of secondary metabolites and compounds that substantially affect the metabolism of the host plant. harzianic acid is a trichoderma secondary metabolite, showing antifungal and ...201323909277
effects of fungicides and biofungicides on population density and community structure of soil oribatid mites.to compare the side-effects of chemical versus biofungicides on non-target organisms in agricultural soil, a study of population structure, spatial distribution and fecundity of oribatid mites, a diverse and species-rich group of microarthropods indicative of decomposer activity in soil was done. plots laid out in agricultural fields of a research station in egypt, were cultivated with cucumber and treated with two chemical fungicides: ridomil plus 50% wp (active ingredients=metalaxyl and copper ...201423933448
biological control of alternaria fruit rot of chili by trichoderma species under field conditions.trichoderma strains were evaluated under field conditions to assay their efficacy in suppressing alternaria fruit rot disease and promoting chili plant growth. the experiment was conducted at the botanical garden, rajshahi university, bangladesh from july 2006 to march 2007. application of trichoderma harzianum imi 392432 significantly (p = 0.05) suppressed the disease compared to alternaria tenuis (t2) treatment and improved both growth and yield. the treatment t4 (t. harzianum imi-392432 + a. ...201023956637
in vitro antagonism of cotton seedlings fungi and characterization of chitinase isozyme activities in trichoderma harzianum.the antagonistic fungus trichoderma harzianum is widely recognized as a potential biocontrol agent against several soil-borne plant pathogens. t. harzinum is rich source of chitinoltic enzymes. in vitro screening of 5 isolates of t. harzinum, one isolate of chaetomium globosum and one isolate of conetherium mentance, revealed that all of them had reduced growth area of macrophomina phaseolina, fusarium solani and rhizoctonia solani on pda medium, significantly. the inhibition percentage ranged f ...201023961072
in vitro antagonistic characteristics of bacilli isolates against trichoderma spp. and three species of mushrooms.twenty isolates of bacillus species obtained from livestock manure composts and cotton-waste composts were tested for their antagonistic effects in vitro against three green mold pathogens of mushrooms (trichoderma harzianum, t. koningii, and t. viridescens). however, there exists a possibility bacillus species may have antagonistic effects against mushrooms themselves, and thus the same 20 isolates were tested in vitro against three species of mushrooms (flammulina velutipes, lentinus edodes, a ...200823997638
[formation peculiarities of quercus robur l. acorn mycobiota under conditions of kyiv polissya].the structure of mycobiota (epiphytic and from inner tissues) of q. robur acorns (580 samples) under conditions of kyiv polissya was studied. acorn samples were collected from oak trees during vegetation period. the epiphytes and mycobiota isolated from inner tissues of acorns was represented by 38 and 20 species, respectively. the majority of fungi were isolated in july. cosmopolitan and non-specific species acremonium sp., alternaria alternata, cladosporium cladosporioides, trichoderma harzian ...201324006787
leaf microbiota of strawberries as affected by biological control agents.the increasing use of biological control agents (bcas) against botrytis cinerea in strawberry raises the question of whether there are any undesirable impacts of foliar applications of bcas on nontarget microorganisms in the phyllosphere. therefore, our objective was to investigate this issue within a field study. strawberry plants were repeatedly sprayed with three bcas-namely, rhizovital 42 fl. (bacillus amyloliquefaciens fzb42), trianum-p (trichoderma harzianum t22), and naturalis (beauveria ...201324020904
greenhouse evaluation of bacillus subtilis ap-01 and trichoderma harzianum ap-001 in controlling tobacco diseases.two biological control agents, bacillus subtilis ap-01 (larminar(™)) and trichoderma harzianum ap-001 (trisan(™)) alone or/in combination were investigated in controlling three tobacco diseases, including bacterial wilt (ralstonia solanacearum), damping-off (pythium aphanidermatum), and frogeye leaf spot (cercospora nicotiana). tests were performed in greenhouse by soil sterilization prior to inoculation of the pathogens. bacterial-wilt and damping off pathogens were drenched first and followed ...200824031219
production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation.many trichoderma species are able to produce 6-pentyl-α-pyrone (6-pp), a lactone with coconut-like aroma. in the present work, several culture parameters were studied to enhance the production of 6-pp by trichoderma harzianum 4040 in solid-state fermentation. green coir powder added to a nutrient solution was used as support material for fermentation. a plackett-burman screening technique was applied, followed by a fractionary factorial design. the best culture conditions within the experimental ...200824031295
inoculation of tomato seedlings with trichoderma harzianum and arbuscular mycorrhizal fungi and their effect on growth and control of wilt in tomato seedlings.a green house study was conducted to investigate the ability of an isolate of trichoderma harzianum (p52) and arbuscular mycorrhizal fungi (amf) in enhancing growth and control of a wilt pathogen caused by fusarium oxysporum f. sp. lycopersici in tomato seedlings. the plants were grown in plastic pots filled with sterilized soils. there were four treatments applied as follows; p52, amf, amf + p52 and a control. a completely randomized design was used and growth measurements and disease assessmen ...201124031662
biological control of rice brown spot with native isolates of three trichoderma species.brown spot caused by bipolaris oryzae is an important rice disease in southern coast of caspian sea, the major rice growing region in iran. a total of 45 trichoderma isolates were obtained from rice paddy fields in golestan and mazandaran provinces which belonged to trichoderma harzianum, t. virens and t. atroviride species. initially, they were screened against b. oryzae by antagonism tests including dual culture, volatile and nonvolatile metabolites and hyperparasitism. results showed that tri ...201224031832
solid state production of polygalacturonase and xylanase by trichoderma species using cantaloupe and watermelon rinds.different solid state fermentation (ssf) sources were tested such as cantaloupe and watermelon rinds, orange and banana peels, for the production of polygalacturonase (pg) and xylanase (xyl) by trichoderma harzianum and trichoderma virens. the maximum production of both pg and xyl were obtained by t. harzianum and t. virnes grown on cantaloupe and watermelon rinds, respectively. time course, moisture content, temperature, ph, supplementation with carbon and nitrogen sources were optimized to ach ...201324037654
media optimization for laccase production by trichoderma harzianum zf-2 using response surface methodology.trichoderma harzianum zf-2 producing laccase was isolated from decaying samples from shandong, china, and showed dye decolorization activities. the objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. the interactions between different fermentation parameters for laccase production were characterized using a plackett-burman design and the response surface methodology. the different media components were initially optimized using t ...201324043124
systemic resistance to gray mold induced in tomato by benzothiadiazole and trichoderma harzianum t39.gray mold (botrytis cinerea) is an important disease of tomato (solanum lycopersicum). this study examined defense-related gene expression involved in the resistance to b. cinerea that is induced in tomato plants by benzothiadiazole and trichoderma harzianum t39 soil drench. in whole plants, transcriptional changes related to salicylic acid and ethylene were induced by the application of a 0.01% benzothiadiazole solution, whereas changes related to jasmonic acid were induced by the application o ...201424047252
production of soluble crude protein using cellulolytic fungi on rice stubble as substrate under waste program management.the investigation was undertaken to enhance the decomposition process by pre-treatment of rice stubble, having higher concentration of lignin. air-dried rice stubble was treated with 1.8 liter of 1% naoh and autoclaved. six cellulolytic fungi, trichoderma harzianum, penicillium citrinum, curvularia lunata, aspergillus flavus and alternaria alternata were grown in basal synthetic medium along with delignified rice-residue as carbon source for production of soluble crude protein. though the loss o ...200524049491
cloning and heterologous expression of serine protease sl41 related to biocontrol in trichoderma harzianum.serine proteases are highly conserved among fungi and considered to play a key role in different aspects of fungal biology. these proteases are involved in fungal growth and have been related to biocontrol processes. to assess the functional role of serine proteases from trichoderma harzianum t88, an effective biocontrol agent, on inhibition of phytopathogenic fungi, a gene (sl41) encoding a serine protease was isolated by 5' and 3' race (rapid amplification of cdna ends). northern blot analysis ...201324060651
identification and characterization of ethanol utilizing fungal flora of oil refinery contaminated soil.the indigenous fungal flora of three oil refinery contaminated sites (bharuch, valsad and vadodara) of india has been documented in the present investigation. a total seventy-five fungal morphotypes were isolated from these sites and out of them, only fifteen isolates were capable of utilizing ethanol (0-8%; v:v) as a sole source of carbon and energy for growth. ten percent ethanol was completely lethal for the growth of all the isolated fungus. biochemical characterization of the potent ethanol ...201424068535
harzianolide, a novel plant growth regulator and systemic resistance elicitor from trichoderma harzianum.a detailed understanding of the effect of natural products on plant growth and protection will underpin new product development for plant production. the isolation and characterization of a known secondary metabolite named harzianolide from trichoderma harzianum strain sqr-t037 were described, and the bioactivity of the purified compound as well as the crude metabolite extract in plant growth promotion and systemic resistance induction was investigated in this study. the results showed that harz ...201324080397
the stereochemical course of tricho-acorenol biosynthesis.the biosynthesis of tricho-acorenol in trichoderma harzianum was investigated by feeding stereospecifically deuterated mevalonolactone isotopomers, followed by a detailed gc/ms analysis of the incorporation of labelling. the results establish a highly stereospecific hydride migration and antarafacial attack of water in the terminal step towards tricho-acorenol.201324084795
truncation of a mannanase from trichoderma harzianum improves its enzymatic properties and expression efficiency in trichoderma reesei.to obtain high expression efficiency of a mannanase gene, thman5a, cloned from trichoderma harzianum mgq2, both the full-length gene and a truncated gene (thman5aδcbm) that contains only the catalytic domain, were expressed in trichoderma reesei qm9414 using the strong constitutive promoter of the gene encoding pyruvate decarboxylase (pdc), and purified to homogeneity, respectively. we found that truncation of the gene improved its expression efficiency as well as the enzymatic properties of the ...201424162722
antifungal cyclic lipopeptides from bacillus amyloliquefaciens strain bo5a.a bioassay-guided fractionation of bacillus amyloliquefaciens strain bo5a afforded the isolation of two new cyclic lipopeptides (1 and 2) as the major lipid constituents (>60%) of the chcl3-meoh (2:1) extract. the chemical structures of the isolated metabolites were elucidated by spectroscopic methods, including 1d and 2d nmr spectroscopy, mass spectrometry (ms), secondary ion mass spectrometry (ms1, ms2), and chemical degradation. the compounds are members of the surfactins family and are based ...201324164115
rhizosphere competent microbial consortium mediates rapid changes in phenolic profiles in chickpea during sclerotium rolfsii infection.the present study was carried out with the aim of evaluating the effectiveness and potentiality of three compatible rhizosphere microbes, viz., fluorescent pseudomonas aeruginosa (phu094), trichoderma harzianum (thu0816) and mesorhizobium sp. (rl091), in promoting plant growth and mobilizing phenolic acid biosynthesis in chickpea under challenge of sclerotium rolfsii. the microbes were applied as seed coating in different combinations in two experimental sets and the pathogen was inoculated afte ...201424168925
the anti-fasciolasis properties of silver nanoparticles produced by trichoderma harzianum and their improvement of the anti-fasciolasis drug triclabendazole.recently, new strains of fasciola demonstrated drug resistance, which increased the need for new drugs or improvement of the present drugs. nanotechnology is expected to open some new opportunities to fight and prevent diseases using an atomic scale tailoring of materials. the ability to uncover the structure and function of biosystems at the nanoscale, stimulates research leading to improvement in biology, biotechnology, medicine and healthcare. the size of nanomaterials is similar to that of m ...201324196355
trichoderma harzianum elicits induced resistance in sunflower challenged by rhizoctonia solani.to investigate the efficacy of trichoderma harzianum nbri-1055 (denoted as 't-1055') in suppression of seedling blight of sunflower caused by rhizoctonia solani kühn and their impact on host defence responses.201424279777
biosynthesis and uptake of copper nanoparticles by dead biomass of hypocrea lixii isolated from the metal mine in the brazilian amazon region.a biological system for the biosynthesis of nanoparticles (nps) and uptake of copper from wastewater, using dead biomass of hypocrea lixii was analyzed and described for the first time. the equilibrium and kinetics investigation of the biosorption of copper onto dead, dried and live biomass of fungus were performed as a function of initial metal concentration, ph, temperature, agitation and inoculum volume. the high biosorption capacity was observed for dead biomass, completed within 60 min of c ...201324282549
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