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enhanced production of laccase from coriolus versicolor ncim 996 by nutrient optimization using response surface methodology.plackett and burman design criterion and central composite design were applied successfully for enhanced production of laccase by coriolus versicolor ncim 996 for the first time. plackett and burman design criterion was applied to screen the significance of ten nutrients on laccase production by c. versicolor ncim 996. out of the ten nutrients tested, starch, yeast extract, mnso(4), mgso(4) x 7h(2)o, and phenol were found to have significant effect on laccase production. a central composite desi ...200818459071
prediction of biodegradation rate constants of hydroxylated polychlorinated biphenyls by fungal laccases from trametes versicolor and pleurotus ostreatus.quantitative structure-activity relationship (qsar) models for fungal laccase-catalyzed degradation of different hydroxylated polychlorinated biphenyls (oh-pcbs) were developed using some fundamental quantum chemical descriptors. the cross-validated q(2)(cum )values for the two optimal qsar models are as high as 0.958 and 0.961 for laccases from trametes versicolor and pleurotus ostreatus, respectively, indicating good predictive abilities for laccase-catalyzed degradation of oh-pcbs. results fr ...200818461267
[preparation and biological properties of basidiomycete aqueous extracts and their mycelial compositions].the basidiomycetes ganoderma lucidum, hericium erinaceus, lentinus edodes and trametes versicolor were used for preparation of aqueous extracts. a polysaccharide preparation (vpg) was isolated from the g. lucidum aqueous extracts. the mycelium was grown under submerged conditions according to an original procedure. preliminary exposure of mice with tumors to cyclosphosphamide in a low dose for prolonged elimination of t-suppressors and rapid recovery of t-killers induced an increase in the effic ...200718461802
biodegradation of endocrine-disrupting phthalates by pleurotus ostreatus.biodegradation of endocrine-disrupting phthalates [diethyl phthalate (dep), dimethyl phthalate (dmp), butylbenzyl phthalate (bbp)] was investigated with 10 white rot fungi isolated in korea. when the fungal mycelia were added together with 100 mg/l of phthalate into yeast extract-malt extract-glucose (ymg) medium, pleurotus ostreatus, irpex lacteus, polyporus brumalis, merulius tremellosus, trametes versicolor, and t. versicolor mrp1 and mrp13 (transformant of the mn-repressed peroxidase gene of ...200818467874
chemical constituents of stereum subtomentosum and two other birch-associated basidiomycetes: an interspecies comparative study.metabolites of the wood-rotting fungus stereum subtomentosum pouzar (basidiomycetes, order russulales, family stereaceae) occurring on birch (betula pendula roth) trees were phytochemically investigated for the first time. three main metabolite chemotypes present in meoh extracts of the fruit bodies, viz. steroids, fatty acids, and water-soluble sugars, were fractionated, isolated, and identified by 1d/2d nmr-spectroscopic analyses, nmr data comparisons, and chemical correlations combined with g ...200818493960
kinetics of redox polymer-mediated enzyme electrodes.oxygen-reducing enzyme electrodes are prepared from laccase of trametes versicolor and a series of osmium-based redox polymer mediators covering a range of redox potentials from 0.11 to 0.85 v. experimentally obtained current density generated by the film electrodes is analyzed using a one-dimensional numerical model to obtain kinetic parameters. the bimolecular rate constant for mediation is found to vary with mediator redox potential from 250 s(-1) m(-1) when mediator and enzyme are close in r ...200818540577
determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity.beech and pine wood blocks were treated with 1,3-dimethylol-4,5-dihydroxyethylen urea (dmdheu) to increasing weight percent gains (wpg). the resistance of the treated specimens against trametes versicolor and coniophora puteana, determined as mass loss, increased with increasing wpg of dmdheu. metabolic activity of the fungi in the wood blocks was assessed as total esterase activity (tea) based on the hydrolysis of fluorescein diacetate and as heat or energy production determined by isothermal m ...200818542949
removal of color from kraft mill wastewaters with cultures of white-rot fungi and with immobilized mycelium of coriolus versicolor.screening fifteen strains of white-rot fungi for their ability to decolorize combined bleached kraft effluent showed that coriolus versicolor in liquid culture removed over 60% of the color of the effluent within six days in the presence of sucrose. treatment of the same effluent with this fungus, immobilized in beads of calcium alginate gel, resulted in 80% decolorization after three days in the presence of sucrose. caustic extraction e(1) effluent was also decolorized by the immobilized fungus ...198318551550
peroxidase-catalyzed color removal from bleach plant effluent.effluent from the caustic extraction stage of a bleach plant is highly colored due to the presence of dissolved products from lignin chlorination and oxidation. color removal from the effluent by hydrogen peroxide at neutral ph was catalyzed by addition of horseradish peroxidase. the catalysis with peroxidase (20 mg/l) was observed over a wide range of peroxide concentrations (0.1mm-500mm), but the largest effect was between 1mm and 100mm. the ph optimum for catalysis was around 5.0, while the b ...198418553342
pulp bleaching using laccase from trametes versicolor under high temperature and alkaline conditions.kraft pulp was delignified using laccase produced by the white rot fungus trametes versicolor immobilized in solid support under specific conditions. the stability tests showed that this enzyme was stable for 6 h at 55 degrees c and ph 8.0, allowing its use under ph and temperature conditions very close to those used in industrial bleaching. in this work, unbleached hardwood kraft pulp was submitted to prebleaching using 2 u laccase/g pulp basis. reaction time, temperature, and ph of the enzymat ...199818576061
combined submerged and solid substrate fermentation for the bioconversion of lignocellulose.a novel two-stage bioreactor has been designed for a combined submerged (sf) and solid substrate fermentation (ssf) of wheat straw. the straw was pretreated with steam, and cellulases from the culture fluid of trichoderma reesei were adsorbed on it for increased bioconvertibility. ssf was conducted in the top part of the bioreactor by inoculating the straw with a 36-h mycelial culture of t. reesei, or coriolus versicolor. in the bottom part of the fermenter, endomycopsis fibuliger was grown in s ...198718581310
investigation of biosensor signal bioamplification: comparison of direct electrochemistry phenomena of individual laccase, and dual laccase-tyrosinase copper enzymes, at a sonogel-carbon electrode.direct electrochemistry of trametes versicolor laccase (lac) was found at a sonogel-carbon electrode. the bioamplification, performed by dual immobilization of this enzyme and mushroom tyrosinase (tyr), of the bio-electrocatalytic reduction of o(2) was investigated. the calculated alpha transfer coefficients were 0.64 and 0.67, and the heterogeneous electron-transfer rate constants were 6.19 and 8.52 s(-1), respectively, for the individual lac and dual lac-tyr-based nafion/sonogel-carbon bio-ele ...200818585223
affinity chromatography as a rapid and convenient method for purification of fungal laccases.a rapid and convenient method for graduation, isolation, and purification of laccase from trametes versicolor and fomes fomentarius culture fluids was developed. for purification affinity chromatography on syringyl- and vanillyl-controlled porosity glass (cpg) columns was applied. the purified laccase of f. fomentarius was immobilized on porous glass. some properties of the immobilized enzyme in comparison to the free one are discussed.199118600674
polyphenoloxidases immobilized in organic gels: properties and applications in the detoxification of aromatic compounds.gelatine gels originate from water in oil microemulsions in which the ternary system consists of isooctane/ sulfosuccinic acid bis [2-ethyl hexyl] ester/water; the solubilization of gelatin in the water pool of these microemulsions transforms them into viscous gels in which it is possible to cosolubilize various reactive molecules. these gels were used to immobilize two phenoloxidases, a laccase from trametes versicolor and a tyrosinase from mushroom. the best balance between gel retention and c ...199518623526
modeling the enzymatic transformation of 3,5-dimethoxy,4-hydroxy cinnamic acid by polyphenoloxidase from the white-rot fungus trametes versicolor.a mechanism for transforming sinapic acid by a polyphenoloxidase from trametes versicolor was investigated using changes in sinapic acid and oxygen concentrations during the reaction. the experiments were performed in a closed system without supplemental oxygen. the effects of temperature and initial oxygen concentration on the reaction rates were examined. to compare the obtained results with those from spectrophotometric studies, some runs were performed using an open system with supplemental ...199618624358
biomimetic synthesis and characterisation of protein capped silver nanoparticles.a controlled and up-scalable route for the biosynthesis of silver nanopartilces (nps) mediated by fungal proteins of coriolus versicolor has been undertaken for the first time. the fungus when challenged with silver nitrate solution accumulated silver nps on its surface in 72h which could be reduced to 1h by tailoring the reaction conditions. under alkaline conditions, the reaction was much faster and could easily proceed at room temperature even without stirring. the resulting ag nps displayed ...200918625550
evaluation of widely consumed botanicals as immunological adjuvants.many widely used botanical medicines are claimed to be immune enhancers. clear evidence of augmentation of immune responses in vivo is lacking in most cases. to select botanicals for further study based on immune enhancing activity, we study them here mixed with antigen and injected subcutaneously (s.c.). globo h and gd3 are cell surface carbohydrates expressed on glycolipids or glycoproteins on the cell surface of many cancers. when conjugated to keyhole limpet hemocyanin (klh), mixed with an i ...200818640165
tolerance to wood preservatives by copper-tolerant wood-rot fungi native to south-central chile.understanding the effect of heavy metals and wood preservatives on the growth of wood-rot fungi native to a certain region may improve reliability in determining the effectiveness of antifungal products, particularly when dealing with new formulations. in this investigation, strains of copper-tolerant wood-rot fungi native to south-central chile were evaluated against two preservatives: commercial chromated copper arsenate type c (cca-c) and a new formulation with boron and silicon (bs). thirtee ...200918654748
purification of recombinant laccase from trametes versicolor in pichia methanolica and its use for the decolorization of anthraquinone dye.a recombinant laccase from trametes versicolor in pichia methanolica was produced constitutively in a defined medium. the recombinant laccase was purified using ultrafiltration, anion-exchange chromatography, and gel filtration. the molecular weight of the purified laccase was estimated as 64 kda by sds-page. the purified recombinant laccase decolorized more than 90% of remazol brilliant blue r (rbbr) initially at 80 mg l(-1) after 16 h at 45 degrees c and ph 5 when 25 u laccase ml(-1) was used. ...200818688574
synthesis and evaluation of n-alkyl-beta-d-glucosylamines on the growth of two wood fungi, coriolus versicolor and poria placenta.various glucosylamines were synthesized from glucose and different alkyl amine compounds. these amino compounds are beta-d-glucopyranosylamine (gpa), n-ethyl-beta-d-glucopyranosylamine (etgpa), n-butyl-beta-d-glucopyranosylamine (bugpa), n-hexyl-beta-d-glucopyranosylamine (hegpa), n-octyl-beta-d-glucopyranosylamine (ocgpa), n-dodecyl-beta-d-glucopyranosylamine (dogpa), n-(2-hydroxyethyl)-beta-d-glucopyranosylamine (hetgpa) and n,n-di(2-hydroxyethyl)-beta-d-glucopyranosylamine (dhetgpa). they wer ...200818694571
growth of trametes versicolor on phenol.trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. the culture growth and degradation ability dependence on culture medium ph value was observed. the optimal ph value of a liquid czapek salt medium was 6.5. the investigated strain utilized completely 0.5 g/l phenol in 6 days. the dynamics of the phenol degradation process was investigated. the process was characterized by specific growth rate micromax 0.33 h(-1), metabolic coefficient k=4.4, yield coefficient ...200818712560
effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes.the exploration of seven physiologically different white rot fungi potential to produce cellulase, xylanase, laccase, and manganese peroxidase (mnp) showed that the enzyme yield and their ratio in enzyme preparations significantly depends on the fungus species, lignocellulosic growth substrate, and cultivation method. the fruit residues were appropriate growth substrates for the production of hydrolytic enzymes and laccase. the highest endoglucanase (111 u ml(-1)) and xylanase (135 u ml(-1)) act ...200818716810
enhanced expression of laccase during the degradation of endocrine disrupting chemicals in trametes versicolor.a putative laccase cdna from a white-rot basidiomycete, trametes versicolor, that consisted of 1,769 nucleotides was cloned using the rapid amplification of cdna ends (race)-pcr method. the deduced amino acid sequence had 4 putative copper binding regions, which are common to fungal laccases. in addition, the sequence was 57-97% homologous to sequences of other t. versicolor laccases. additionally, the expression of laccase and manganese peroxidase in this fungus were both greatly increased unde ...200818758730
production and structural analysis of the polysaccharide secreted by trametes (coriolus) versicolor atcc 200801.trametes versicolor atcc 200801 secretes 4.1 g l(-1) of exopolysaccharide (eps) when synthetic minimal medium and low-shear bioreactor cultivation technique are used. structural and compositional analyses by thin layer chromatography, gas chromatography-mass spectrometry, electrospray ionization tandem mass spectrometry, and nuclear magnetic resonance spectroscopy yielded predominantly glucose and small amounts of galactose, mannose, arabinose, and xylose. the main eps is composed of beta-1,3/be ...200918800181
protein-bound polysaccharide-k (psk) directly enhanced igm production in the human b cell line ball-1.protein-bound polysaccharide-k (psk) prepared from the basidiomycete coriolus versicolor has been used as a biological response modifier for the treatment of cancer patients. many studies describing the immunomodulatory effects and direct anti-cancer effects of psk have been reported. most of studies describing the immunomodulatory effects focused on cellular immunity, although there were several studies which focused on humoral immunity where psk was shown to be able to induce antibody producti ...200918848763
polysaccharopeptide mimics ciclosporin-mediated th1/th2 cytokine balance for suppression of activated human t cell proliferation by mapkp38 and stat5 pathways.the activation of t helper (th) cell subsets plays an important role in the human immune system. uncontrolled th1 and th2 responses lead to autoimmune and inflammatory diseases, respectively. the identification of agents that modulate the th1/th2 cytokines is therefore essential for controlling these diseases. we recently reported that polysaccharopeptide (psp) from coriolus versicolor exhibited ciclosporin-like activities to control aberrant t lymphocyte activation. here, we compared the proper ...200818957170
protoplast fusion betweenlentinula edodes andcoriolus versicolor.protoplast fusion between isoleucine-, arginine- and thymidine-requiring auxotroph (lle, arg, thy) oflentinula edodes and arginine-requiring auxotroph (arg) ofcoriolus versicolor has been achieved using 30% polyethylene glycol (m.w. 4000) in 10 mmcacl(2)-glycine solution (ph 8.0). fusion hybrids were selected in the 0.6 m sucrose supplemented minimal media on the basis of nutritional complementation with fusion frequency of 7.4x10(-6). the hybrids included both parental and non-parental types in ...199718982488
fungal post-treatment of pulp mill effluents for the removal of recalcitrant pollutants.the objective of this work was to evaluate the post-treatment of an anaerobic recalcitrant effluent (anaerobically-treated weak black liquor, ane) in an aerobic, upflow reactor packed with "biocubes" of trametes versicolor immobilized onto small cubes of holm oak wood. the treated effluent (named anaerobic effluent; ane) from an anaerobic fluidized bed reactor was fed to an up-flow aerobic fungal packed bed reactor (pbr). two hrt were tested in this unit, namely 5 and 2.5days; the pbr operated 6 ...200919010664
biodegradation and saccharification of wood chips of pinus strobus and liriodendron tulipifera by white rot fungi.degradation and glucose production from wood chips of white pine (pinus strobus) and tulip tree (liriodendron tulipifera) by several white rot fungi were investigated. the highest weight losses from 4 g of wood chips of p. strobus and l. tulipifera by the fungal degradation on yeast extractmalt extract-glucose agar medium were 38% of irpex lacteus and 93.7% of trametes versicolor mrp 1 after 90 days, respectively. when 4 g of wood chips of p. strobus and l. tulipifera biodegraded for 30 days wer ...200819047827
characterization of radical intermediates in laccase-mediator systems. a multifrequency epr, endor and dft/pcm investigation.suitable low molecular-weight compounds, called mediators, can be used in combination with the phenol-oxidase enzyme laccase to indirectly oxidize large organic substrates, such as environmental pollutants, which are not laccase natural substrates. the oxidation of two different synthetic redox mediators, violuric acid (vio) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (abts) has been studied under catalysis of two laccases from white-rot fungi (trametes versicolor and pleurotus o ...200819060974
ability of white-rot fungi to remove selected pharmaceuticals and identification of degradation products of ibuprofen by trametes versicolor.a screening using four white-rot fungi (trametes versicolor, irpex lacteus, ganoderma lucidum and phanerochaete chrysosporium) was performed on the degradation of 10 mg l(-1) of ibuprofen (ibu, anti-inflammatory), clofibric acid (clofi, lipid regulator) and carbamazepine (carba, antiepileptic/analgetic) after 7 d of incubation. whereas ibu was extensively degraded by all the fungi tested, t. versicolor was the only strain able to degrade either clofi (approximately 91%) and carba (approximately ...200919062071
biological method to quantify progressive stages of decay in five commercial woods by coriolus versicolor.biologic agar-block method was developed that allowed wood samples to be evaluated and monitored in terms of colonization and development of the decay by basidiomycetes fungi (coriolus versicolor) and to be directly classified based on mean mass loss. in this research, the in vitro decay of five commercial woods by coriolus versicolor was studied by the agar-block method. the selected wood samples were abies alba, populus alba, fagus orientalis, platanus orientalis and ulmus glabra. the results ...200719070053
determination of phenolic acids using trametes versicolor laccase.two biosensors based on trametes versicolor laccase (tvl) were developed for the determination of phenolic compounds. commercial oxygen electrode and ferrocene-modified screen-printed graphite electrodes were used for preparation of laccase biosensors. the systems were calibrated for three phenolic acids. linearity was obtained in the concentration range 0.1-1.0mum caffeic acid, 0.05-0.2mum ferulic acid, 2.0-14.0mum syringic acid for laccase immobilised on a commercial oxygen electrode and 2.0-3 ...200719071305
statistical optimization for decolorization of textile dyes using trametes versicolor.the conventional treatment of dark coloured textile wastewater using chemical coagulation generates large volume of sludge, which requires further treatment and disposal. in the present investigation, a systematic optimization study of the important variables influencing the decolorization of reactive orange-16 (ro-16) and reactive red-35 (rr-35) dyes by the white-rot fungus (trametes versicolor) was carried out. a full factorial central composite design was employed for experimental design and ...200919081186
trametes versicolor mushroom immune therapy in breast cancer.data from multiple epidemiologic and clinical studies on immune effects of conventional cancer treatment and the clinical benefits of polysaccharide immune therapy suggest that immune function has a role in breast cancer prevention. immune therapy utilizing the polysaccharide constituents of trametes versicolor (tv) as concurrent adjuvant cancer therapy may be warranted as part of a comprehensive cancer treatment and secondary prevention strategy.200819087769
pyranose 2-oxidase (p2o): production from trametes versicolor in stirred tank reactor and its partial characterization.optimization of pyranose-2-oxidase (p2o) production conditions from trametes versicolor was carried out in shaking cultures containing glucose, malt, and yeast extracts; the optimum concentration values were found to be 1.5% glucose, 1.0% yeast extract, and 1.0% malt extract, ph 5.0, temperature, 26 degrees c, and agitation rate 150 rpm. for the first time, p2o production was also carried out in a stirred tank reactor (str) with 2.2 l working volume in the optimized medium composition, and bioma ...200919090419
removal of estrogenic activity of iso-butylparaben and n-butylparaben by laccase in the presence of 1-hydroxybenzotriazole.in the presence of a redox mediator, 1-hydroxybenzotriazole (hbt), iso-butylparaben (iso-bp) and n-butylparaben (n-bp) were treated with laccase from white rot fungus trametes versicolor. hplc analysis demonstrated that iso-bp and n-bp almost completely disappeared from the reaction mixture after 4 h of treatment with the laccase-hbt system. using the yeast two-hybrid assay system, it was also confirmed that the laccase-hbt system substantially removed the estrogenic activity of iso-bp and n-bp ...200919109757
biofuel cell and phenolic biosensor based on acid-resistant laccase-glutaraldehyde functionalized chitosan-multiwalled carbon nanotubes nanocomposite film.to immobilize laccase (lac) from trametes versicolor that shows its maximum enzymatic activity in acidic aqueous solutions, the biopolymer chitosan (cs) was chemically modified with glutaraldehyde (ga) to form ga functionalized cs (gafcs), which was then allowed to react with lac to form a lac-gafcs composite that is robust in weakly acidic solutions (two-step protocol), as confirmed by quartz crystal microbalance and durability tests. the lac-gafcs-multiwalled carbon nanotubes (mwcnts)/glassy c ...200919153037
dechlorination of 1,2,3- and 1,2,4-trichlorobenzene by the white-rot fungus trametes versicolor.the degradation of 1,2,3-, 1,3,5- and 1,2,4-trichlorobenzene (tcb) by the white-rot fungus trametes versicolor was studied. time course experiments showed a degradation rate of 2.27 and 2.49 nmol d(-1)mg(-1) dry weight of biomass during the first 4d of incubation in cultures spiked with 6 mg l(-1) of 1,2,3- and 1,2,4-tcb, respectively. a high percent of degradation of 91.1% (1,2,3-tcb) and 79.6 (1,2,4-tcb) was obtained after 7d. however, t. versicolor was not able to degrade 1,3,5-tcb under the ...200919179004
in situ biodecolorization kinetics of acid red 66 in aqueous solutions by trametes versicolor.the biological decolorization methodology and the mechanism involved in the degradation of acid red 66 was chosen as a model of azo dye using the white rot fungi trametes versicolor was studied. biodecolorization of acid red 66 using white rot fungi t. versicolor was demonstrated by the decolorization of the culture medium, the extent of which was determined by monitoring the decrease in absorbance at 506 nm. the biodecolorization kinetics of acid red 66 (100 ppm) was found to be first order and ...200919201090
isolation and screening of natural organic matter-degrading fungi.drinking water quality and its treatment are negatively impacted by the presence of coloured natural organic matter (nom) derived from the breakdown of animal and plant materials. ligninolytic fungi (i.e., white rot fungi - wrf) secrete non-specific oxidative enzymes that can oxidise a wide range of recalcitrant organic compounds. the potential for these organisms to decolourise concentrated aquatic nom was investigated. twenty-one isolates from diverse fungal genera were screened using nom plat ...200919233448
biodegradation of endocrine-disrupting compounds and suppression of estrogenic activity by ligninolytic fungi.endocrine-disrupting compounds (edcs) represent a large group of substances of natural and anthropogenic origin. they are widely distributed in the environment and can pose serious risks to aquatic organisms and to public health. in this study, 4-n-nonylphenol, technical 4-nonylphenol, bisphenol a, 17alpha-ethinylestradiol, and triclosan were biodegraded by eight ligninolytic fungal strains (irpex lacteus 617/93, bjerkandera adusta 606/93, phanerochaete chrysosporium me 446, phanerochaete magnol ...200919243809
induction of an oxalate decarboxylase in the filamentous fungus trametes versicolor by addition of inorganic acids.in order to improve yields and to reduce the cost of oxalate decarboxylase (oxdc, ec 4.1.1.2), the induction of oxdc in the white-rot fungus trametes versicolor was studied in this work. oxdc was induced by addition of inorganic acids including hydrochloric acid, sulfuric acid, and phosphoric acid to culture media. the results showed that all the acids could enhance oxdc expression. the activity of the acid-induced oxdc rose continuously. all of the oxdc volumetric activities induced by the inor ...201019266319
biological treatment of the effluent from a bleached kraft pulp mill using basidiomycete and zygomycete fungi.three white-rot fungi (pleurotus sajor caju, trametes versicolor and phanerochaete chrysosporium) and one soft-rot fungi (rhizopus oryzae) species confirmed their potential for future applications in the biological treatment of effluents derived from the secondary treatment of a bleached kraft pulp mill processing eucalyptus globulus. among the four species p. sajor caju and r. oryzae were the most effective in the biodegradation of organic compounds present in the effluent, being responsible fo ...200919269018
metabolites from the biodegradation of triphenylmethane dyes by trametes versicolor or laccase.the feasibility of degrading triphenylmethane dyes by trametes versicolor and laccase has been investigated for the following dyes: acid fuchsin, brilliant green 1, basic fuchsin, methyl green or acid green 16. the toxicity level of triphenylmethane dyes is linked to their basic character, but significant detoxification is obtained when there is biodegradation. identification of enzymatic degradation products by (1)h nmr made it possible to propose a general rule for the laccase attack on triphe ...200919298999
mediator facilitated, laccase catalysed oxidation of granular potato starch and the physico-chemical characterisation of the oxidized products.the primary hydroxyl groups in potato starch were selectively oxidized to the corresponding aldehyde and carboxylic acid functionalities by mediators like tempo, using laccase from fungi as catalytic oxidant and oxygen as the primary oxidant. oxidized starch products with degree of substitution (ds(cho) ranging from 0.16 to 16.4/100agu and ds(cooh) from 0.01 to 3.71 carboxyl groups/100agu) were obtained with mediator facilitated enzymatic oxidation. maximum conversion of the primary alcohol grou ...200919299125
effect of low doses of guaiacol and ethanol on enzymatic activity of fungal cultures.the influence of low doses of guaiacol and ethanol, the natural effectors of lignin and phenolics transformations, on laccase and peroxidase activities produced by two strains of basidiomycetes, pleurotus sajor-caju and trametes versicolor, was evaluated. fungal mycelia were grown for 2 weeks on liquid media containing serial dilutions of guaiacol or ethanol ranging from 100(-1) to 100(-20) mol/l. laccase and peroxidase activities in the medium were measured at the end of 2 weeks. the effect of ...200319330120
induction of extracellular hydroxyl radical production by white-rot fungi through quinone redox cycling.a simple strategy for the induction of extracellular hydroxyl radical (oh) production by white-rot fungi is presented. it involves the incubation of mycelium with quinones and fe(3+)-edta. succinctly, it is based on the establishment of a quinone redox cycle catalyzed by cell-bound dehydrogenase activities and the ligninolytic enzymes (laccase and peroxidases). the semiquinone intermediate produced by the ligninolytic enzymes drives oh production by a fenton reaction (h(2)o(2) + fe(2+) --> oh + ...200919376892
biodegradation of brominated aromatics by cultures and laccase of trametes versicolor.2-bromophenol (1), 4-bromophenol (2), 2,4-dibromophenol (3), 2,6-dibromophenol (4), 2,4,6-tribromophenol (5) and tetrabromobisphenol a (6) (1 mm each) added to growing submerged cultures of trametes versicolor ccbas 612 were eliminated by 65-85% from the culture medium within 4d. extracellular laccase activity in the culture medium was influenced by the type of brominated compound added. maximum level of laccase (63 u l(-1)) was found in the culture with 2-bromophenol. tetrabromobisphenol a was ...200919443012
[coriolus versicolor--innovation in prevention of oncogynecological diseases, especially hpv].coriolus-mrl is a nutrient adjuvant, which contains biomass of the fungus coriolus versicolor and is studied to reverse early stages of cervical cancer and to reduce risk factors of reoccurring hpv virus.200819449722
modification of wheat straw lignin by solid state fermentation with white-rot fungi.the potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (trametes versicolor, bjerkandera adusta, ganoderma applanatum and phlebia rufa), was exploited to modify wheat straw cell wall. at different fermentation times, manganese-dependent peroxidase (mnp), lignin peroxidase (lip), laccase, carboxymethylcellulase (cmcase), avicelase, xylanase and feruloyl esterase activities were screened and the content of lignin as well as hydroxycinnamic acids in fe ...200919450975
improving the catalytic performance of fungal laccases in monoterpene-based reaction systems.ternary systems consisting of monoterpenes (alpha-pinene or d-limonene), tert-butanol and water were used as reaction media to enhance the catalytic performance of laccases from various fungi sources (trametes versicolor, t. hirsuta and botrytis cinerea). the enzymes had improved catalytic efficiency (5- to 10-fold) in alpha-pinene-rich environment, while optimal reaction rates were in high-water content systems (15.5% v/v). the stability of laccases was significantly improved in monoterpene-bas ...200919458921
fungal bioremediation of chromates: conformational changes of biomass during sequestration, binding, and reduction of hexavalent chromium ions.this paper highlights the mechanistic aspects of white rot fungus coriolus versicolor as a complexing/reducing agent for chromium bioremediation. the chemical reduction of cr(vi) to cr(iii) via the formation of cr(vi) thio ester as an intermediate, is ph dependent and controls the overall chromium adsorption kinetics. the strong adsorption affinity of the biomass towards cr(vi) anions was evaluated by the freundlich and the langmuir adsorption isotherms. the ftir spectroscopic analysis suggested ...200919467785
gravimetric screening method for fungal decay of paper: inoculation with trametes versicolor.the european standard test en 113 for fungal degradation of solid wood has been adapted for degradation of paper by white rot fungus (trametes versicolor). fungal degradation of paper sheets may potentially be used for screening different wood preservatives on paper instead of solid wood. the paper samples showed higher relative mass losses compared to wood, and samples pretreated with boric acid, copper sulfate and polymerized linseed oil were successfully tested for biodegradation using the pa ...200919495565
white-rot basidiomycete-mediated decomposition of c60 fullerol.industrially produced carbon-based nanomaterials (cnm), including fullerenes and nanotubes, will be introduced into the environment in increasing amounts in the next decades. one likely environmental chemical transformation of c60 is oxidation to c60 fullerol through both abiotic- and biotic-mediated means. unfortunately, knowledge of the environmental fate of oxidized cnm is lacking. this study used bulk and compound-specific 13c stable isotope ratio mass spectrometry techniques and spectroradi ...200919534129
oxidation of anthracene by immobilized laccase from trametes versicolor.the laccase of trametes versicolor was immobilized on the functionalized nanoparticles sba-15 with the average diameter less than 10 nm. laccase mediated oxidations of anthracene (ant) were investigated in the presence of two mediators, 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (abts) and 1-hydroxybenzotriazole (hbt). oxidation of ant was more efficiently enhanced by adding 1 mm of hbt than that by adding abts. after 48 h oxidation hbt group significantly oxidized ...200919564104
are stag beetles fungivorous?stag beetle larvae generally feed on decaying wood; however, it was unknown whether they can use wood-rotting fungi alone as food. here, to clarify this, newly hatched larvae of dorcus rectus (motschulsky) (coleoptera: lucanidae) were reared for 14 days on artificial diets containing a fixed amount of freeze-dried mycelia of the following fungi: bjerkandera adusta, trametes versicolor, pleurotus ostreatus, and fomitopsis pinicola. the mean incremental gain in larval body mass was greatest on die ...200919607834
induction of hydroxyl radical production in trametes versicolor to degrade recalcitrant chlorinated hydrocarbons.extracellular hydroxyl radical ((*)oh) production via quinone redox cycling in trametes versicolor, grown in a chemically defined medium, was investigated to degrade trichloroethylene (tce), perchloroethylene (pce), 1,2,4- and 1,3,5-trichlorobenzene (tcb). the activity of the enzymes catalyzing the quinone redox cycle, quinone reductase and laccase, as well as the rate of (*)oh production, estimated as the formation of thiobarbituric acid reactive substances (tbars) from 2-deoxyribose, increased ...200919616936
oxidation of the erythro and threo forms of the phenolic lignin model compound 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)-1,3-propanediol by laccases and model oxidants.mixtures of equal amounts of the erythro and threo forms of the phenolic arylglycerol beta-aryl ether 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)-1,3-propanediol were oxidized (i) with laccases from trametes versicolor, agaricus bisporus, myceliophthora thermophila and rhus vernicifera, (ii) with laccase-mediator systems consisting of t. versicolor laccase and abts or hbt, and (iii) with various model oxidants including cerium(iv) ammonium nitrate (can), lignin peroxidase, fenton's reagen ...200919646732
contribution of the saprobic fungi trametes versicolor and trichoderma harzianum and the arbuscular mycorrhizal fungi glomus deserticola and g. claroideum to arsenic tolerance of eucalyptus globulus.the presence of high concentrations of arsenic (as) decreased the shoot and root dry weight, chlorophyll and p and mg content of eucalyptus globulus colonized with the arbuscular mycorrhizal (am) fungi glomus deserticola or g. claroideum, but these parameters were higher than in non-am plants. as increased the percentage of am length colonization and succinate dehydrogenase (sdh) activity in the root of e. globulus. trichoderma harzianum, but not trametes versicolor, increased the shoot and root ...200919648001
surface display of redox enzymes in microbial fuel cells.a novel concept for a biofuel cell is presented. enzyme based fuel cells suffer from enzyme instability when a long time of operation is required. hence, a system that will continuously produce the biocatalyst needed for the system is necessary. a hybrid of an enzyme-based microbial fuel cell was developed. the redox enzyme glucose oxidase from aspergillus niger was displayed on the surface of saccharomyces cerevisiae using the yeast surface display system in a high copy number and as an active ...200919663383
degradation of aflatoxin b(1) by fungal laccase enzymes.the enzymatic degradation of aflatoxin b(1) (afb(1)) by white rot fungi through laccase production was investigated in different liquid media. a significant (p<0.0001) correlation was observed between laccase activity and afb(1) degradation exhibited by representatives of peniophora and pleurotus ostreatus cultivated in minimal salts (msm) (r=0.93) and mineral salts - malt extract (msb-meb) (r=0.77) liquid media. peniophora sp. scc0152 cultured in msb-meb liquid medium supplemented with veratryl ...200919683355
interactions of trametes versicolor, coriolopsis rigida and the arbuscular mycorrhizal fungus glomus deserticola on the copper tolerance of eucalyptus globulus.the presence of high levels of cu in soil decreases the shoot and root dry weights of eucalyptus globulus. however, higher plant tolerance of cu has been observed in the presence of the arbuscular mycorrhizal (am) fungus glomus deserticola. the hyphal length of g. deserticola was sensitive to low cu concentrations, and the percentage of am root colonisation and the metabolic activity of the am fungus were also decreased by cu. therefore, a direct effect of cu on the development of the am fungus ...200919692112
three-dimensional x-ray imaging and analysis of fungi on and in wood.as wood is prone to fungal degradation, fundamental research is necessary to increase our knowledge aiming at product improvement. several imaging modalities are capable of visualizing fungi, but the x-ray equipment presented in this article can envisage fungal mycelium in wood nondestructively in three dimensions with submicron resolution. four types of wood subjected to the action of the white rot fungus coriolus versicolor (linnaeus) quélet (ctb 863 a) were scanned using an x-ray-based approa ...200919709462
increase of laccase activity during interspecific interactions of white-rot fungi.white-rot fungi are of interest due to their ability to degrade lignin. lignin-degrading enzymes such as laccase can also degrade xenobiotic compounds. the effects of interspecific interactions between white-rot fungi and other microorganisms on laccase activity was studied in laboratory cultures. laccase activity in cultures of trametes versicolor and pleurotus ostreatus increased significantly after the introduction of soil fungi, bacteria and yeasts or after contact with nonsterile soil. addi ...200419712364
influence of very low doses of mediators on fungal laccase activity - nonlinearity beyond imagination.abstract: laccase, an enzyme responsible for aerobic transformations of natural phenolics, in industrial applications requires the presence of low-molecular substances known as mediators, which accelerate oxidation processes. however, the use of mediators is limited by their toxicity and the high costs of exploitation. the activation of extracellular laccase in growing fungal culture with highly diluted mediators, abts and hbt is described. two high laccase-producing fungal strains, trametes ver ...200919732425
degradation of phenols in olive oil mill wastewater by biological, enzymatic, and photo-fenton oxidation.olive oil mill wastewater (oomw) environmental impacts minimization have been attempted by developing more effective processes, but no chemical or biological treatments were found to be totally effective to mitigate their impact on receiving systems. this work is the first that reports simultaneously the efficiency of three different approaches: biological treatment by two fungal species (trametes versicolor or pleurotus sajor caju), enzymatic treatment by laccase, and chemical treatment by phot ...201019841956
oxidation of atenolol, propranolol, carbamazepine and clofibric acid by a biological fenton-like system mediated by the white-rot fungus trametes versicolor.biological advanced oxidation of the pharmaceuticals clofibric acid (ca), carbamazepine (cbzp), atenolol (atl) and propranolol (ppl) is reported for the first time. extracellular oxidizing species were produced through a quinone redox cycling mechanism catalyzed by an intracellular quinone reductase and any of the ligninolytic enzymes of trametes versicolor after addition of the lignin-derived quinone 2,6-dimethoxy-1,4-benzoquinone (dbq) and fe(3+)-oxalate in the medium. time-course experiments ...201019850317
modifications of amino acids during ferulic acid-mediated, laccase-catalysed cross-linking of peptides.mass spectral analysis demonstrated oligomerization of peptides that had been subjected to oxidation catalysed by trametes (coriolus) versicolor laccase. peptide oligomerization occurred only when cysteines or tyrosines were present in the peptides. ms/ms confirmed the cross-linking in tyrosine-containing peptides to be located between tyrosine residues. ferulic acid mediated oligomerization of cysteine-containing peptides, but prevented cross-linking of tyrosines when used in the same concentra ...200919905979
white-rot fungus-mediated degradation of the analgesic ketoprofen and identification of intermediates by hplc-dad-ms and nmr.ketoprofen is a nonsteroidal anti-inflammatory drug that has been detected in the environment in the range of ng l(-1)-microg l(-1) due to its low degradability in some wastewater treatment plants. in this study, the use of the white-rot fungus trametes versicolor to effectively degrade ketoprofen in a defined liquid medium was assessed. the fungus eliminated ketoprofen to nondetectable levels in 24h when it was added at 10mgl(-1) whereas at low concentration of 40microgl(-1) it was almost compl ...201019913277
docking simulation and competitive experiments validate the interaction between the 2,5-xylidine inhibitor and rigidoporus lignosus laccase.laccases are polyphenol oxidases which oxidize a broad range of reducing substrates, preferably phenolic compounds, and their use in biotechnological applications is increasing. recently, the first x-ray structure of active laccase from white rot fungus rigidoporus lignosus has been reported containing a full complement of copper ions. comparison among selected fungal laccases of known 3d structure has shown that the rigidoporus lignosus laccase has a very high similarity with the trametes versi ...201019916571
[impact of exogenous paraquat on enzyme exudation and biochemical changes of lignin degradation fungi].to study the effect of exogenous oxygen, we added water solution of paraquat to 7 d cultures of coriolus versicolor for the next 148 h. enzyme exudation and biochemical process were investigated on the addition of paraquat. we found that compared with the control (without paraquat), the addition of 30 micromol/l paraquat stimulated the activity of manganese dependent peroxidase (mnp), lignin peroxidase (lip), and laccases (lac) 7, 2.5 and 1.3 times, respectively. also, addition of paraquat enhan ...200919938450
mathematical model for trametes versicolor growth in submerged cultivation.trametes versicolor is a white-rot fungus known as a producer of extracellular enzymes such as laccase, manganese-peroxidase, and lignin-peroxidase. the production of these enzymes requires detailed knowledge of the growth characteristics and physiology of the fungus. submerged cultivations of t. versicolor on glucose, fructose, and sucrose as sole carbon sources were performed in shake flasks. sucrose hydrolysis catalyzed by the whole cells of t. versicolor was considered as one-step enzymatic ...201019949814
biodegradation of the analgesic naproxen by trametes versicolor and identification of intermediates using hplc-dad-ms and nmr.the white-rot fungus trametes vesicolor degraded naproxen (10 mg l(-1)) in a liquid medium to non-detectable levels after 6h. when naproxen was added in the range of concentrations typically found in the environment (55 microg l(-1)), it was almost completely degraded (95%) after 5h. in vitro degradation experiments with purified laccase and purified laccase plus mediator 1-hydroxybenzotriazol showed slight and almost complete naproxen degradation, respectively. a noticeable inhibition on naprox ...201020004093
degradation of the drug sodium diclofenac by trametes versicolor pellets and identification of some intermediates by nmr.degradation of diclofenac sodium, a nonsteroidal anti-inflammatory drug widely found in the aquatic environment, was assessed using the white-rot fungus trametes versicolor. almost complete diclofenac removal (> or = 94%) occurred the first hour with t. versicolor pellets when the drug was added at relatively high (10 mg l(-1)) and environmentally relevant low (45 microg l(-1)) concentrations in a defined liquid medium. in vivo and in vitro experiments using the cytochrome p450 inhibitor 1-amino ...201020031320
degradation of naproxen and carbamazepine in spiked sludge by slurry and solid-phase trametes versicolor systems.growth and activity of the white-rot fungus trametes versicolor on sewage sludge were assessed in bioslurry and solid-phase systems. bioslurry cultures with different loads of sludge (10%, 25% and 38%, w/v) were performed. a lag phase of at least 2 d appeared in the 25 and 38%-cultures, however, the total fungal biomass was higher for the latter and lower for the 10%-culture after 30 d, as revealed by ergosterol determination. detectable laccase activity levels were found in the 10 and 25%-cultu ...201020031398
kinetic and biochemical properties of high and low redox potential laccases from fungal and plant origin.the electrochemical studies of laccase-mediator systems are aimed at understanding the mechanism of their redox transformation and their efficiency in both homogeneous and heterogeneous reactions; this topic has paramount application spanning from bleaching of paper pulp and the enzymatic degradation of lignin to the biosensors and biofuel cell development. in this paper four different laccases from trametes hirsuta (thl), trametes versicolor (tvl), melanocarpus albomyces (r-mal) and rhus vernic ...201020056172
effects of tertiary treatment by fungi on organic compounds in a kraft pulp mill effluent.pulp and paper mills generate a plethora of pollutants depending upon the type of pulping process. efforts to mitigate the environmental impact of such effluents have been made by developing more effective biological treatment systems in terms of biochemical oxygen demand, chemical oxygen demand, colour and lignin content. this study is the first that reports an evaluation of the effects of a tertiary treatment by fungi (pleurotus sajor caju, trametes versicolor and phanerochaete chrysosporium a ...201020101467
host preference and species richness of wood-inhabiting aphyllophoraceous fungi in a cool temperate area of japan.we examined the species richness and host utilization patterns of wood-inhabiting aphyllophoraceous fungi (polypores and related fungi) in an old-growth beech and oak forest in a cool, temperate area of japan. coarse woody debris (cwd) > or = 20 cm diam within a 6 ha plot was surveyed in sep 2002. tree genus, diameter, decay class and tree part of cwd samples were recorded. fruiting bodies of aphyllophoraceous fungi that arose from the cwd were surveyed three times and identified to species. in ...201020120223
cresols utilization by trametes versicolor and substrate interactions in the mixture with phenol.the ability of the white rot fungus trametes versicolor strain 1 to degrade and utilize methylated phenols (cresols) was established for the first time in a medium not containing any other carbon components. the data obtained demonstrated the better potential of the strain to assimilate p-cresol instead of o- or m- cresol. the 0.5 g/l p-cresol provided was degraded in full after 96 h. the effect of a dual substrate mixture (0.3 g/l phenol + 0.2 g/l p-cresol) on the growth behavior and degradatio ...201020127146
design of reaction conditions for the enhancement of microbial degradation of dyes in sequential cycles.the present study evaluated the potential of white-rot fungal strain coriolus versicolor to decolorize five structurally different dyes in sequential batch reactors under optimized conditions. the experiments were run continuously for seven cycles of 8 d each. high decolorizing activity was observed even during the repeated reuse of the fungus, especially when the old medium was replaced with fresh medium after every cycle. biodegradation was the dominating factor as the fungus was able to produ ...200920131593
immunomodulatory effects of polysaccharopeptide (psp) in human pbmc through regulation of traf6/tlr immunosignal-transduction pathways.polysaccharopeptide (psp) was extracted from coriolus versicolor, and has been proved to be a valuable adjuvant for the combination with chemotherapy or radiotherapy in the treatment of various cancers. objective: to understand the mechanism of psp on immunomodulation, we examined gene expression and cytokine secretion associated with immunosignal-transduction signaling in human peripheral blood mononuclear cells (pbmcs).201020131955
application of laccase-mediator system (lms) for the degradation of organophosphorus compounds.degradation of organophosphorus compounds was achieved in the presence of purified fungal laccase from trametes versicolor and a small molecular weight redox mediator (abts). this laccase-mediator system (lms) catalyzed degradation of vx, phx and vr while had no apparent effect on cvx, ecothiophate or demeton. inhibition of abts oxidation was shown with vx, phx, vr and cvx. results with cvx suggest either no degradation subsequent to interaction with the laccase active site or the formation of a ...201020149786
effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. dye decolorization by selected culture filtrates.the effect of amino acids, complex nitrogen sources and vitamin addition on trametes trogii, trametes villosa and coriolus versicolor var. antarcticus ligninolytic enzyme production, was evaluated. dye decolorization by their culture filtrates was compared. glutamic acid followed by peptone, were the best n sources for laccase and manganese peroxidase production. the three fungi produced two laccase isoenzymes (molecular weights from 38 up to 150 kda); their pattern of production was not affecte ...201020153961
optimization of removal conditions of copper ions from aqueous solutions by trametes versicolor.a multi-step response surface methodology was successfully applied to optimize the biosorption conditions for the maximum removal of cu(ii) ions from aqueous solutions using trametes versicolor fungi as a biosorbent. in the first step, the most effective medium factors, which are ph, temperature and initial cu(ii) concentration, on biosorption of cu(ii), were determined through plackett-burman design. then steepest accent followed by central composite design steps were utilized to evaluate the o ...201020156677
influence of iron and copper nanoparticle powder on the production of lignocellulose degrading enzymes in the fungus trametes versicolor.white rot fungi are one of the key group of microorganisms that help to enrich the soil via degradation of wood. in the current communication, influence of iron and copper nanoparticles on the production of lignocellulolytic enzymes by trametes versicolor have been investigated. the production of enzymes in the presence of the two nanoparticles was compared to that of ferrous and cupric ions respectively. results show that both the tested nanoparticles alter the production profile of the lignoce ...201020185234
laccase-based biosensor for the determination of polyphenol index in wine.in this work we have developed and characterized the use of laccases from trametes versicolor (tvl) and trametes hirsuta (thl) as biocatalytic components of electrochemical biosensors for the determination of polyphenol index in wines. polyazetidine prepolimer (pap) was used as immobilizing agent, multi-walled and single-walled carbon nanotubes screen-printed electrodes as sensors (mwcnts-spe and swcnts-spe) and gallic acid as standard substrate. the amperometric measurements were carried out by ...201020188914
investigation of growth responses in saprophytic fungi to charred biomass.we present the results of a study testing the response of two saprophytic white-rot fungi species, pleurotus pulmonarius and coriolus versicolor, to charred biomass (charcoal) as a growth substrate. we used a combination of optical microscopy, scanning electron microscopy, elemental abundance measurements, and isotope ratio mass spectrometry ((13)c and (15)n) to investigate fungal colonisation of control and incubated samples of scots pine (pinus sylvestris) wood, and charcoal from the same spec ...201020229385
[development of a laminar bioadsorbent with hypertolerant phanerochaete chrysosporium to cd, ni and pb for wastewater treatment].the use of basidiomycetes for metal removal is an alternative to traditional methods. in this, the biomass acts as a natural ionic exchanger removing metals from solution.201020346296
naproxen degradation test to monitor trametes versicolor activity in solid-state bioremediation processes.the white-rot fungus trametes versicolor has been studied as a potential agent for the removal of environmental pollutants. for long-time solid-phase bioremediation systems a test is required to monitor the metabolic status of t. versicolor and its degradation capability at different stages. a biodegradation test based on the percentage of degradation of a spiked model pharmaceutical (anti-inflammatory naproxen) in 24 h (nd24) is proposed to monitor the removal of pharmaceuticals and personal ca ...201020347219
changes in oxidative enzyme activity during interspecific mycelial interactions involving the white-rot fungus trametes versicolor.interspecific fungal antagonism leads to biochemical changes in competing mycelia, including up-regulation of oxidative enzymes. laccase, manganese peroxidase (mnp), manganese-repressed peroxidase (mrp) and lignin peroxidase (lip) gene expression and enzyme activity were compared during agar interactions between trametes versicolor and five other wood decay fungi resulting in a range of interaction outcomes from deadlock to replacement of one fungus by another. increased laccase and mn-oxidising ...201020371297
construction and comparison of trametes versicolor laccase biosensors capable of detecting xenobiotics.amperometric biosensors using laccase from trametes versicolor as a bioelement were developed for 2,4-dichloro phenoxy acetic acid (2,4-d). laccase enzyme was immobilized by gelatin and glutaraldehyde onto a clark oxygen probe and screen printed electrodes (spes). amperometric and chronoamperometric measurements were carried out with the biosensors. first, the effect of laccase activity on the biosensor performances was investigated for both biosensors, and then optimum ph and temperature and al ...201020380615
degradation of triclosan by an integrated nano-bio redox process.in this study, a sequential reduction-oxidation method was developed for complete degradation of triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether, tcs) in aqueous solution. rapid reductive dechlorination of tcs was achieved with palladized zero-valent iron nanoparticles (pd/nfe), under anaerobic conditions, with generation of 2-phenoxyphenol as the sole dechlorination product. sequentially, 2-phenoxyphenol was transformed into a non-toxic polymer using laccase (ec:1.10.3.2) derived from tram ...201020381343
reversible immobilization of laccase to poly(4-vinylpyridine) grafted and cu(ii) chelated magnetic beads: biodegradation of reactive dyes.poly(4-vinyl pyridine), poly(vp), as a novel metal-chelating fibrous polymer was grafted on the magnetic beads. poly(4-vp) grafted and/or cu(ii) ions chelated magnetic beads were used for reversible immobilization of trametes versicolor laccase, and the amounts of immobilized laccase on the beads were determined as 36.8 and 56.4 mg/g beads, respectively. the adsorption of laccase on both modified magnetic beads appeared to follow the langmuir isotherm model. the degradation of textile dyes with ...201020388589
[enhancement of laccase activity by combining white rot fungal strains].the method of combining white rot fungal strains was used to enhance laccase activity, and the interaction mechanism between strains was also studied. the laccase activity of combined fungi of strain 55 (trametes trogii) and strain m-6 (trametes versicolor) were 24.13 and 4.07-fold higher than that of strain 55 and strain m-6, respectively. no inhibitory effect was observed when the two strains were co-cultivated. on plate cultivation, there was hyphal interference in the contact area, where lac ...201020391719
polysaccharopeptides derived from coriolus versicolor potentiate the s-phase specific cytotoxicity of camptothecin (cpt) on human leukemia hl-60 cells.abstract:201020423495
amperometric biosensor based on a high resolution photopolymer deposited onto a screen-printed electrode for phenolic compounds monitoring in tea infusions.an amperometric biosensor based on laccase, from trametes versicolor (ltv), was developed and optimized for monitoring the phenolic compounds content in tea infusions. the fungal enzyme was immobilized by entrapment within polyvinyl alcohol photopolymer pva-awp (azide-unit pendant water-soluble photopolymer) onto disposable graphite screen-printed electrodes (spe). sensitivity optimization in terms of ph, temperature and applied potential was carried out. the linear range, detection limit, opera ...201020441951
the effect of combination enzymatic and advanced oxidation process treatments on the colour of pulp and paper mill effluent.the efficiency of advanced oxidation processes (aops), enzymatic treatment and combined enzymatic/aop sequences for the colour remediation of soda and chemimechanical pulp and paper mill effluent was investigated. the results indicated that under all circumstances, the aop using ultraviolet irradiation (photo-fenton) was more efficient in the degradation of effluent components in comparison with the dark reaction. it was found that both versatile peroxidase (vp) from bjerkandera adusta and lacca ...201020450108
comparison of effectiveness of wood decay fungi maintained by annual subculture on agar and stored in sterile water for 18 years.fourteen isolates of basidiomycete decay fungi (12 species) were maintained for 18 years on agar slants transferred annually and also stored as mycelium-agar cores under cold sterile water without subculture. isolates stored by each method were evaluated for decay effectiveness using a standard laboratory accelerated soil-block decay test. effectiveness was measured by mean percent mass loss of wood blocks. there was no significant difference (p < or = 0.05) in decay effectiveness between storag ...201020453914
biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review.one of the major challenges faced in commercial production of lignocellulosic bioethanol is the inhibitory compounds generated during the thermo-chemical pre-treatment step of biomass. these inhibitory compounds are toxic to fermenting micro-organisms. the ethanol yield and productivity obtained during fermentation of lignocellulosic hydrolysates is decreased due to the presence of inhibiting compounds, such as weak acids, furans and phenolic compounds formed or released during thermo-chemical p ...201120513164
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