Publications

inulinase-producing marine yeasts: evaluation of their diversity and inulin hydrolysis by their crude enzymes.	total 427 yeast strains from seawater, sediments, mud of salterns, guts of the marine fish, and marine algae were obtained. after inulinase activity of the yeast cultures was estimated, we found that four strains (ouc1, g7a, ouc2, and g7a1) of the marine yeasts grown in the medium with inulin could secrete a large amount of inulinase into the medium. the results of routine identification and molecular methods show that they belong to pichia guilliermondii ouc1, cryptococcus aureus g7a, yarrowia ...	2007	17345128
[the pleiotropic nature of rib80, hit1, and red6 mutations affecting riboflavin biosynthesis in the yeast pichia guilliermondii].	the yeast pichia guilliermondii is capable of riboflavin overproduction under iron deficiency. the rib80, hit1, and red6 mutants of this species, which exhibit impaired riboflavin regulation, are also distinguished by increased iron concentrations in the cells and mitochondria, morphological changes in the mitochondria, as well as decreased growth rates (except for red6) and respiratory activity. with sufficient iron supply, the rib80 and red6 mutations cause a 1.5-1.8-fold decrease in the activ ...	2007	17410876
dynamics and characterization of yeasts during ripening of typical italian dry-cured ham.	the evolution of the yeast population during manufacturing and ripening of dry-cured parma ham was investigated. contamination levels ranged from 10(5) to 10(7) cfu/g on muscle surface, 10(4) to 10(6) cfu/g on covering fat and exceeded 10(7) cfu/g on spreadable fat mince ("sugna"). two hundred and sixty one yeast isolates underwent identification test, showing that the predominant species of yeast population during the whole maturing process were debaryomyces hansenii, candida zeylanoides, debar ...	2007	17418308
bioconversion of ferulic acid to 4-vinylguaiacol and 4-ethylguaiacol and of 4-vinylguaiacol to 4-ethylguaiacol by halotolerant yeasts belonging to the genus candida.	in order to examine the genesis of the characteristic flavors of soy sauce and miso, seven novel halotolerant yeast strains of two types, which showed convertibility of ferulic acid (fa) to 4-vinylguaiacol (4-vg) and to 4-ethylguaiacol (4-eg), were isolated from miso-koji and miso pastes. two of these strains were identified as candida guilliermondii (anamorph of pichia guilliermondii), and candida fermentati (anamorph of pichia caribbica), based on sequence analyses of a partial 26s ribosomal r ...	2007	17420598
biotechnological production of xylitol in a three-phase fluidized bed bioreactor with immobilized yeast cells in ca-alginate beads.	cells of candida guilliermondii entrapped in ca-alginate beads were used for xylitol production, from concentrated hemicellulose hydrolyzate of sugarcane bagasse, in a fluidized bed bioreactor (fbr). the maximum xylitol concentration 28.9 g xylitol/l was obtained at a high aeration rate of 600 ml/min after 70 h of fermentation, indicating that the use of high aeration rate in this system is favored for better oxygen transfer into the immobilized cells. the specific xylitol productivity and the x ...	2007	17427994
development of a transformation system for gene knock-out in the flavinogenic yeast pichia guilliermondii.	pichia guilliermondii is a representative of a yeast species, all of which over-synthesize riboflavin in response to iron deprivation. molecular genetic studies in this yeast species have been hampered by a lack of strain-specific tools for gene manipulation. stable p. guilliermondii ura3 mutants were selected on the basis of 5'-fluoroorotic acid resistance. plasmid carrying saccharomyces cerevisiae ura3 gene transformed the mutant strains to prototrophy with a low efficiency. substitution of a ...	2007	17467833
evaluation of albicans id2 and biggy agar for the isolation and direct identification of vaginal yeast isolates.	in this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, albicans id(2) and biggy agar, as well as onto sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. these strains were identified as candida glabrata in 20 (31.8 %) samples, candida albicans in 15 samples (23.8 %), candida tropicalis in 10 samples (15.9 %), candida krusei in five samples (7.9 %), candida kefyr in five samples (7.9 %), cand ...	2007	17510260
disseminated infection due to candida guilliermondii in a patient with aml(m4). case study.	a 43-year-old patient admitted with acute myelogenous leukemia, developed bronchopneumonia and sepsis during profound neutropenia. fever and pulmonary infiltrates did not improve by using empiric antibacterial therapy (cefoperazona-sulbactam, trimethoprim-sulphametoxazol). blood and sputum culture were performed and patient received voriconazol. both cultures, from blood and sputum, yielded candida guilliermondii after 48 hours of incubations. the isolates have the same biochemical and antimicro ...	2006	17571574
isolation and characterization of novel peptides from chilli pepper seeds: antimicrobial activities against pathogenic yeasts.	different types of antimicrobial peptides have been identified in seeds from different plant species. the aim of this study was to isolate and characterize peptides present in chilli pepper seeds (capsicum annuum l.) and evaluate their toxic activities against some yeast species. initially, proteins from seed flour were extracted in phosphate buffer, ph 5.4, for 3 h at 4 degrees c and the pellet obtained at 90% saturation with ammonium sulfate was heated at 80 degrees c for 15 min. the resulting ...	2007	17572465
comparison of a randomly amplified polymorphic dna (rapd) analysis and atb id 32c system for identification of clinical isolates of different candida species.	the objective of this work was to compare the usefulness of a randomly amplified polymorphic dna (rapd) assay to that of the atb id32c kit (biomérieux, france) for identification of different species of candida isolated from clinical specimens. the rapd-pcr patterns obtained with ope-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. rapd with the ope-18 primer is a very specific and sensitive method ...	2007	17604435
l-arabinose transport and catabolism in yeast.	two yeasts, candida arabinofermentans pycc 5603(t) and pichia guilliermondii pycc 3012, which show rapid growth on l-arabinose and very high rates of l-arabinose uptake on screening, were selected for characterization of l-arabinose transport and the first steps of intracellular l-arabinose metabolism. the kinetics of l-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. the l-arabinose catab ...	2007	17627668
yeast diversity in rice-cassava fermentations produced by the indigenous tapirapé people of brazil.	the tapirapé people of the tapi'itãwa tribe of brazil produce several fermented foods and beverages, one of which is called 'cauim'. this beverage usually makes up the main staple food for adults and children. several substrates are used in its production, including cassava, rice, corn, maize and peanuts. a fermentation using rice and cassava was conducted, and samples were collected at 4-h intervals for microbial analysis. the yeast population was low at the beginning of the fermentation and re ...	2007	17697080
[useful phenotypic characteristics for presumptive identification of candida guilliermondii].	candida guilliermondii developed a pink-purplish colony on chromagar candida. in the micromorphology in milk-tween 80 1% agar at 28 degrees c after 48 h of incubation c. guilliermondii showed small (3-5 microm), spherical yeasts without pseudohyphaes. this candida species presented a characteristic cluster of blastospores with pseudohyphaes radiating from the centre at 96 h. the trehalose-sucrose assimilation assay was applied to the c. guilliermondii isolates which proved negative for trehalose ...	2007	17702251
production of ethanol from soybean hull hydrolysate by osmotolerant candida guilliermondii nrrl y-2075.	in this research, we studied the use of soybean hull hydrolysate (shh) as a substrate for ethanol and xylitol production using an osmotolerant strain of candida guilliermondii. the best acid hydrolysis of soybean hull achieved a recovery of 85 and 62% of xylose and mannose, respectively. among detoxification treatments, activated charcoal 10% (w/v) showed the best results. kinetic parameters obtained from the cultivation on four-fold concentrated shh have shown that the osmotic pressure of this ...	2008	17706417
the pan-ac assay: a single-reaction real-time pcr test for quantitative detection of a broad range of aspergillus and candida species.	in view of the growing incidence and the high mortality of invasive aspergillosis and candidiasis, adequate diagnostic techniques permitting timely onset of treatment are of paramount importance. more than 90 % of all invasive fungal infections in immunocompromised individuals can be attributed to candida and aspergillus species. to date, standardized techniques permitting rapid, sensitive and, no less importantly, economic screening for the clinically most relevant fungi are lacking. in the pre ...	2007	17761478
correlation of posaconazole minimum fungicidal concentration and time kill test against nine candida species.	we evaluated the in vitro activity of posaconazole against nine candida species using minimum fungicidal concentration (mfc) measurements and time-kill methods.	2007	17875607
fungemia due to candida guilliermondii in a pediatric and adult population during a 12-year period.	candida guilliermondii fungemia is usually described in adults with hematologic malignancies, but in children, only 2 episodes have been published. from 1995 to 2006, 7 episodes (5 in children) were detected in our hospital. molecular typing excluded a common infection source. c. guilliermondii fungemia may occur in children with underlying conditions other than cancer.	2008	17889486
mutations and environmental factors affecting regulation of riboflavin synthesis and iron assimilation also cause oxidative stress in the yeast pichia guilliermondii.	iron deficiency causes oversynthesis of riboflavin in several yeast species, known as flavinogenic yeasts. however, the mechanisms of such regulation are not known. we found that mutations causing riboflavin overproduction and iron hyperaccumulation (rib80, rib81 and hit1), as well as cobalt excess or iron deficiency all provoke oxidative stress in the pichia guilliermondii yeast. iron content in the cells, production both of riboflavin and malondialdehyde by p. guilliermondii wild type and hit1 ...	2007	17910100
efficacy of chromogenic candida agar for isolation and presumptive identification of pathogenic yeast species.	chromogenic candida agar is a novel differential culture medium that is claimed to facilitate isolation and identification of candida albicans, candida tropicalis and candida krusei. the performance of this medium was evaluated for presumptive identification of 521 yeast strains, representing 23 different species, for detection of specimens containing yeast mixtures, and for direct isolation of yeast from blood cultures. all yeasts grew well on the medium following a 48-h incubation period at 37 ...	2008	17986267
cell immobilization and xylitol production using sugarcane bagasse as raw material.	sugarcane bagasse pretreated by three different procedures (with 2% [v/v] polyethyleneimine (pei), with 2% [w/v] naoh, or with a sequence of naoh and pei) was used as cell immobilization carrier for xylitol production by candida guilliermondii yeast. fermentations using these pretreated carriers were performed in semidefined medium and in a hydrolysate medium produced from sugarcane bagasse hemicellulose. sugarcane bagasse pretreated with naoh was the best carrier obtained with respect to immobi ...	2007	18025553
phase diagrams of a ctab/organic solvent/buffer system applied to extraction of enzymes by reverse micelles.	a partial pseudo-ternary phase diagram has been studied for the cethyltrimethylammonium bromide/isooctane:hexanol:butanol/potassium phosphate buffer system, where the two-phase diagram consisting of the reverse micelle phase (l2) in equilibrium with the solvent is indicated. based on these diagrams two-phase systems of reverse micelles were prepared with different compositions of the compounds and used for extraction and recovery of two enzymes, and the percentage of enzyme recovery yield monito ...	2008	18054293
comparative in vitro activities of caspofungin and micafungin, determined using the method of the european committee on antimicrobial susceptibility testing, against yeast isolates obtained in france in 2005-2006.	the in vitro activities of caspofungin and micafungin against 1,038 yeast isolates have been determined. the caspofungin and micafungin mics were lower for candida albicans, candida glabrata, and candida tropicalis than for candida parapsilosis, candida guilliermondii, and candida krusei. a clear correlation was seen between the mics for the two drugs.	2008	18070978
determination of killer activity in yeasts isolated from the elaboration of seasoned green table olives.	in this work 51 yeasts strains isolated from seasoned green table olives and belonging to the candida, debaryomyces, kluyveromyces, pichia, and saccharomyces genera were characterized by their killer activity in different conditions. killer activity of isolates was analyzed in a medium with different ph's (3.5 to 8.5) and nacl concentrations (5, 8, and 10%). at every ph tested, all the genera studied had killer strains, although the smallest percentages of killer yeasts were found at the highest ...	2008	18077043
[feasibilities of photodynamic therapy in the treatment of corneal mycotic lesions: an experimental study].	the paper presents the results of studying the photofungicidal activity of a photosens (octacationic phthalocyanine) in the in vitro and in vivo systems. cultured candida guilliermondii were employed in in vitro investigations. the single combined action of octacationic phthalocyanine at a concentration of up to 1 microm and laser irradiation at a wavelength of 675 nm were established to allow the high activation (up to 100%) of yeast fungi to be achieved in the in vitro system. in vivo investig ...	2007	18078054
structural analysis of cell wall mannan of candida sojae, a new yeast species isolated from defatted soybean flakes.	we investigated the structural and immunochemical characteristics of cell wall mannan obtained from candida sojae jcm 1644, which is a new yeast species isolated from defatted soybean flakes. the results of a slide-agglutination test and of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic candida species indicated that the cells and the c. sojae mannan were cross-reactive to the specific anti-factor sera against candida albicans serotype a (fab 6) and candida guillie ...	2008	18084740
dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses.	an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ...	2008	18160452
semi-continuous xylose-to-xylitol bioconversion by ca-alginate entrapped yeast cells in a stirred tank reactor.	candida guilliermondii fti 20037 cells were entrapped in ca-alginate beads and used for xylose-to-xylitol bioconversions during five successive batches in a stirred tank reactor. supplemented sugarcane bagasse hemicellulosic hydrolysate was used as the fermentation medium. the average volume of the ca-alginate beads was reduced by about 30% after the 600 h taken to perform the five bioconversion cycles, thus demonstrating physical instability under the conditions prevailing in the reactor vessel ...	2008	18175152
evaluation of the inhibitory effect of dimethyl dicarbonate (dmdc) against wine microorganisms.	several microbial species associated with wine were challenged against increasing concentrations of dimethyl dicarbonate (dmdc). the concentration inducing complete cell death upon addition to red wine was regarded as the minimum inhibitory concentration (mic). in dry red wines with 12% (v/v) ethanol and ph 3.50, the inactivation depended on the initial cell concentration. for an initial inoculum of 500 cfu/ml, the mic of the yeasts species schizosaccharomyces pombe, dekkera bruxellensis, saccha ...	2008	18206786
use of in vivo 13c nuclear magnetic resonance spectroscopy to elucidate l-arabinose metabolism in yeasts.	candida arabinofermentans pycc 5603(t) and pichia guilliermondii pycc 3012 were shown to grow well on l-arabinose, albeit exhibiting distinct features that justify an in-depth comparative study of their respective pentose catabolism. carbon-13 labeling experiments coupled with in vivo nuclear magnetic resonance (nmr) spectroscopy were used to investigate l-arabinose metabolism in these yeasts, thereby complementing recently reported physiological and enzymatic data. the label supplied in l-[2-(1 ...	2008	18245253
re-examining the phylogeny of clinically relevant candida species and allied genera based on multigene analyses.	yeasts of the artificial genus candida include plant endophytes, insect symbionts, and opportunistic human pathogens. phylogenies based on rrna gene and actin sequences confirmed that the genus is not monophyletic, and the relationships among candida species and allied teleomorph genera are not clearly resolved. protein-coding genes have been useful to resolve taxonomic positions among a broad range of fungi. over 70 taxa of the genus candida and its allied sexually reproducing genera were there ...	2008	18248416
efungumab and caspofungin: pre-clinical data supporting synergy.	heat shock protein 90 (hsp90) is targeted by the humoral response in invasive candidiasis. this paper tests for synergy between caspofungin and efungumab--a human antibody fragment against hsp90.	2008	18299636
[successful treatment of azole-refractory candida guilliermondii fungemia with a combination therapy of micafungin and liposomal amphotericin b].	candida guilliermondii (c. guilliermondii) are uncommon, representing approximately 1% of all candida infections, but have been reported to show a higher rate of drug-resistance and mortality rate than c. albicans. current guidelines for treatment of non-albicans candidemia in neutropenic patients now recommend the use of amphotericin b or voriconazole (vrcz). we describe here the successful treatment for a 58-year-old male with azole-refractory c. guilliermondii fungemia by combination with lip ...	2008	18341039
[rapid identification and susceptibility to killer toxins of yeasts isolated from non-systemic mycoses].	rapid identification and susceptibility to killer toxins of yeasts isolated from non-systemic mycoses. the use of quick and reliable yeast identification methods, as well as the development of new antifungal agents with more specific targets, will enable a more efficient treatment of mycoses. in the present work, a total of 53 clinical isolates obtained from non-systemic infections in neuquén hospitals and an ophthalmologic clinic in buenos aires during 2005, were identified by means of a rapid ...	2007	18390160
effect of furfural, vanillin and syringaldehyde on candida guilliermondii growth and xylitol biosynthesis.	xylitol is a five-carbon sugar alcohol with established commercial use as an alternative sweetener and can be produced from hemicellulose hydrolysate. however, there are difficulties with microbiological growth and xylitol biosynthesis on hydrolysate because of the inhibitors formed from hydrolysis of hemicellulose. this research focused on the effect of furfural, vanillin, and syringaldehyde on growth of candida guilliermondii and xylitol accumulation from xylose in a semi-synthetic medium in m ...	2008	18418743
[in vitro antifungal activity of anidulafungin].	anidulafungin is a new and very useful pharmacological tool for the treatment of invasive mycoses. the antifungal spectrum of anidulafungin reaches the most common pathogenic fungi. anidulafungin is especially active against the genera candida and aspergillus. its antifungal mechanism is based on the inhibition of the beta-1,3-d-glucan synthesis, an essential molecule for the cell wall architecture, with different consequences for candida and aspergillus, being anidulafungin fungicide for the fo ...	2008	18473502
amphotericin b-metronidazole combination against candida spp.	oropharyngeal candidiasis (opc) remains a common opportunistic infection in hiv-infected patients. candida albicans is the most frequent causative agent of opc. however, non-albicans spp. are being increasingly isolated. candidal cell wall proteins and mannoproteins play important roles in the biology and patogenesis of candidiasis. in the present study, we have analyzed the proteinaceous components associated with cell wall extracts from c. albicans, candida tropicalis, candida pseudotropicalis ...	1998	18473531
multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from spanish patients.	twelve spanish laboratories collected 325 yeast clinical isolates during a 30 day's period, among them 224 candida albicans, 30 candida glabrata, and 27 candida parapsilosis. in vitro antifungal susceptibility to amphotericin b, ketoconazole, fluconazole and itraconazole was determined by an agar diffusion test (neo-sensitabs, rosco, denmark). all the isolates tested were susceptible in vitroto amphotericin b and nearly all (97.2%) to itraconazole. in vitrosusceptibility to fluconazole and ketoc ...	1999	18473577
preliminary characterization and grouping of candida species by numerical analysis of protein profiles obtained by polyacrylamide gel electrophoresis.	whole-cell proteins from isolates of five candida species (candida albicans, candida krusei, candida parapsilosis, candida tropicalis and candida guilliermondii) were separated by sds-page and the profiles obtained were converted into a binary data matrix that produced a cophenetic correlation phenogram. the analysis of the phenogram allowed detection of the cophenetic correlation levels existing among these species.	1999	18473588
effect of microbial biocontrol agents on alleviating oxidative damage of peach fruit subjected to fungal pathogen.	levels of protein carbonylation in peach fruits inoculated with four antagonistic yeasts (pichia membranaefaciens, cryptococcus laurentii, candida guilliermondii and rhodotorula glutinis) were significantly reduced in response to reactive oxygen species (ros) caused by monilinia fructicola. in control fruit without yeast treatments, proteins carbonylation obviously increased after inoculation with m. fructicola, ranging from molecular mass 20 to 120 kda. however, in yeast-treated fruits, no prot ...	2008	18558450
[the fungicidal activity and paradoxical effect of caspofungin against yeast. influence of culture medium and incubation time].	the effect of culture medium and incubation time on the fungicidal activity of caspofungin and on the frequency of paradoxical effect has been studied in 144 blood culture isolates (42, candida tropicalis, 32 candida glabrata, 21 candida krusei, 20 candida parapsilosis, 11 candida guilliermondii, 4 candida famata, 3 candida lusitaniae, 3 blastoschizomyces capitatus, 2 saccharomyces cerevisiae, 2 yarrowia lipolytica, 1 candida inconspicua, 1 candida lambica, 1 candida sake y 1 pichia ohmeri). the ...	2007	18563217
development of a novel pcr assay for the identification of the black yeast, exophiala (wangiella) dermatitidis from adult patients with cystic fibrosis (cf).	cystic fibrosis (cf) patients may suffer increased morbidity and mortality through colonisation, allergy and invasive infection from fungi. the black yeast, exophiala dermatitidis (synonym wangiella dermatitidis) has been found with increasing frequency in sputum specimens of cf patients, with reported isolation rates ranging from 1.1 to 15.7%. at present, no diagnostic pcr exists to aid with the clinical laboratory detection and identification of this organism. a novel species-specific pcr-base ...	2008	18571996
maximizing the xylitol production from sugar cane bagasse hydrolysate by controlling the aeration rate.	batch fermentations of sugar cane bagasse hemicellulosic hydrolysate treated for removing the inhibitors of the fermentation were performed by candida guilliermondii fti20037 for xylitol production. the fermentative parameters agitation and aeration rate were studied aiming the maximization of xylitol production from this agroindustrial residue. the maximal xylitol volumetric productivity (0.87 g/l h) and yield (0.67 g/g) were attained at 400/min and 0.45 v.v.m. (k(l)a 27/h). according to the re ...	1997	18576110
evidence of recent interkingdom horizontal gene transfer between bacteria and candida parapsilosis.	to date very few incidences of interdomain gene transfer into fungi have been identified. here, we used the emerging genome sequences of candida albicans wo-1, candida tropicalis, candida parapsilosis, clavispora lusitaniae, pichia guilliermondii, and lodderomyces elongisporus to identify recent interdomain hgt events. we refer to these as ctg species because they translate the ctg codon as serine rather than leucine, and share a recent common ancestor.	2008	18577206
multigradient method for optimization of slow biotechnological processes.	a new method (named a "jumping spider") is introduced for the optimization of slow biotechnological processes. the more traditional sequential experimentation (i.e., gradient search, simplex, etc.) is not well suited for slow dynamic processes, e.g., plant cell culture and differentiation. therefore, a more simultaneous approach is proposed. a large number of initial experiments are performed, on the basis of which several of the initial experiments are selected as starting points. a search is t ...	1993	18612958
multiplex tandem pcr: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens.	we describe the first development and evaluation of a rapid multiplex tandem pcr (mt-pcr) assay for the detection and identification of fungi directly from blood culture specimens that have been flagged as positive. the assay uses a short-cycle multiplex amplification, followed by 12 simultaneous pcrs which target the fungal internal transcribed spacer 1 (its1) and its2 region, elongation factor 1-alpha (ef1-alpha), and beta-tubulin genes to identify 11 fungal pathogens: candida albicans, candid ...	2008	18632914
pva-hydrogel entrapped candida guilliermondii for xylitol production from sugarcane hemicellulose hydrolysate.	viable cells of candida guilliermondii were immobilized by inclusion into polyvinyl alcohol (pva) hydrogel using the freezing-thawing method. entrapment experiments were planned according to a 2(3) full factorial design, using the pva concentration (80, 100, and 120 g l(-1)), the freezing temperature (-10, -15, and -20 degrees c), and the number of freezing-thawing cycles (one, three, and five) as the independent variables, integrated with three additional tests to estimate the errors. the effec ...	2009	18633733
debaryomyces hansenii (candida famata), a rare human fungal pathogen often misidentified as pichia guilliermondii (candida guilliermondii).	debaryomyces hansenii is a hemiascomycetous yeast commonly found in natural substrates and in various types of cheese. pichia guilliermondii is widely distributed in nature and is a common constituent of the normal human microflora. both species have been described in human infections but are extremely difficult to differentiate phenotypically. thus, frequent errors in identification occur. the 62 clinical and environmental isolates sent between 2000 and 2007 to the french national reference cen ...	2008	18701668
isolation, characterization and cloning of a cdna encoding a new antifungal defensin from phaseolus vulgaris l. seeds.	the pvd1 defensin was purified from phaseolus vulgaris (cv. pérola) seeds, basically as described by terras et al. [terras frg, schoofs hme, de bolle mfc, van leuven f, ress sb, vanderleyden j, cammue bpa, broekaer twf. analysis of two novel classes of plant antifungal proteins from radish (raphanus sativus l.) seeds. j biol chem 1992;267(22):15301-9], with some modifications. a deae-sepharose, equilibrated with 20mm tris-hcl, ph 8.0, was initially utilized for the separation of peptides after a ...	2008	18786582
evaluation of new colorimetric vitek 2 yeast identification card by use of different source media.	the new colorimetric vitek 2 yst card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. the vitek 2 yst card achieved satisfactory results for all yeast species tested, with the exception of candida guilliermondii, candida norvegensis, candida parapsilosis, candida rugosa, and candida tropicalis. after simple additional tests, 93.7% of all the strains tested were correctly identified. a significant influence of the isolation medium ...	2008	18799700
marine killer yeasts active against a yeast strain pathogenic to crab portunus trituberculatus.	some marine yeasts have recently been recognised as pathogenic agents in crab mariculture, but may be inhibited or killed by 'killer' yeast strains. we screened multiple yeast strains from seawater, sediments, mud of salterns, guts of marine fish, and marine algae for killer activity against the yeast metchnikowia bicuspidata wcy (pathogenic to crab portunus trituberculatus), and found 17 strains which could secrete toxin onto the medium and kill the pathogenic yeast. of these, 5 strains had sig ...	2008	18814546
cloning and characterization of the inulinase gene from a marine yeast pichia guilliermondii and its expression in pichia pastoris.	the extracellular inulinase structural gene was isolated from the genomic dna of the marine yeast pichia guilliermondii strain 1 by pcr. the gene had an open reading frame of 1,542 bp long encoding an inulinase. the coding region of the gene was not interrupted by any intron. it encoded 514 amino acid residues of a protein with a putative signal peptide of 18 amino acids and the calculated molecular mass of 58.04 kda. the protein sequence deduced from the inulinase structural gene contained the ...	2009	18821076
the behavior of key enzymes of xylose metabolism on the xylitol production by candida guilliermondii grown in hemicellulosic hydrolysate.	a variety of raw materials have been used in fermentation process. this study shows the use of rice straw hemicellulosic hydrolysate, as the only source of nutrient, to produce high added-value products. in the present work, the activity of the enzymes xylose reductase (xr); xylitol dehydrogenase (xd); and glucose-6-phosphate dehydrogenase (g6pd) during cultivation of candida guilliermondii on rice straw hemicellulosic hydrolysate was measured and correlated with xylitol production under differe ...	2009	18830730
influence of cacl(2) on penicillium digitatum, grapefruit peel tissue, and biocontrol activity of pichia guilliermondii.	abstract interactions between cacl(2), grapefruit peel tissue, penicillium digitatum, and the yeast antagonist pichia guilliermondii strain us-7 were investigated. application of 68 or 136 mm cacl(2) to grapefruit surface wounds reduced the incidence of green mold caused by penicillium digitatum by 43 to 52%. in laboratory tests, a cell suspension (10(7) cells/ml) of pichia guilliermondii containing either 68 or 136 mm cacl(2) reduced the incidence of green mold from 27 to 3%. in large scale tes ...	1997	18945174
isolation of candida dubliniensis in denture stomatitis.	to describe the isolation of candida dubliniensis from a patient with denture stomatitis and to compare with the presence of yeasts in the oral cavities of denture wearers.	2009	18950745
[in vitro amphotericin b susceptibility of korean bloodstream yeast isolates assessed by the clsi broth microdilution method, etest, and minimum fungicidal concentration test].	although amphotericin b (amb) has a wide spectrum of activity that encompasses the majority of yeast isolates, there have been recent reports suggesting that some yeast isolates exhibit decreased susceptibility to amb. however, in vitro amb susceptibility of yeast species isolates from blood cultures in korea has not been fully surveyed.	2008	18971615
synthesis of a heptasaccharide fragment of the mannan from candida guilliermondii cell wall and its conjugate with bsa.	the 3-aminopropyl glycoside of a heptasaccharide fragment of the cell wall mannan from candida guilliermondii 18, which corresponds to the antigenic factor 9, has been synthesized by a convergent approach based on glycosylation of a tetrasaccharide acceptor with a trisaccharide donor as the key step to give a protected heptasaccharide 17. subsequent two-step deprotection of 17 afforded the heptamannoside 18, which was then conjugated with bsa using the squarate procedure.	2009	18976984
role of glycerol addition on xylose-to-xylitol bioconversion by candida guilliermondii.	the effect of glycerol on xylose-to-xylitol bioconversion by candida guilliermondii was evaluated by its addition (0.7 and 6.5 g/l) to semidefined media (xylose as a substrate). the glycerol concentrations were chosen based on the amounts produced during previous studies on xylitol production by c. guilliermondii. medium without glycerol addition (control) and medium containing glycerol (53 g/l) in substitution to xylose were also evaluated. according to the results, the addition of 0.7 g/l glyc ...	2009	19034573
identification and susceptibility profile of candida fermentati from a worldwide collection of candida guilliermondii clinical isolates.	candida fermentati isolates make up a small percentage of the clinical isolates of the candida guilliermondii complex and have a global distribution pattern. with the exception that the mics of micafungin were significantly lower, the calculated average mics for c. fermentati were not significantly different from those for c. guilliermondii.	2009	19036938
proteinase and phospholipase activity as virulence factors in candida species isolated from blood.	the number of nosocomial blood stream infections due to candida species has increased over the past few decades. in order to establish an infection, opportunistic pathogens have to evade the immune system, survive, divide in the host environment, and spread to other tissues. proteinase and phospholipase secretion has been implicated as potential virulence factors for some candida species responsible for catheter related candidemia in intensive care unit (icu) patients with indwelling devices. we ...	2008	19071887
enhanced inulinase production in solid state fermentation by a mutant of the marine yeast pichia guilliermondii using surface response methodology and inulin hydrolysis.	in order to isolate inulinase overproducers of the marine yeast pichia guilliermondii, strain 1, cells were mutated by using uv light and licl(2). one mutant (m-30) with enhanced inulinase production was obtained. response surface methodology (rsm) was used to optimize the medium compositions and cultivation conditions for inulinase production by the mutant in solid-state fermentation. the initial moisture, inoculum, the amount ratio of wheat bran to rice bran, temperature, ph for the maximum in ...	2009	19107534
[opportunistic yeast infections and enzymatic profile of the etiological agents].	yeast infections are common in immunocompromised patients, although emerging species have been changing the epidemiological profile. the ability to secrete proteinases has been associated with pathogenicity within the genus candida. this study had the aims of diagnosing yeast infections in immunocompromised patients and evaluating the virulence of the etiological agents, based on a proteinase secretion assay using bovine serum albumin as a substrate. out of a total of 104 patients studied, 19 pr ...	2009	19448940
[in vitro antifungal activity of micafungin].	micafungin is a new and very useful pharmacological tool for the treatment of invasive mycoses with a wide antifungal spectrum for the most common pathogenic fungi. micafungin is especially active against the genera candida and aspergillus. its antifungal mechanism is based on the inhibition of the beta-1,3- d-glucan synthesis, an essential molecule for the cell wall architecture, with different con sequences for candida and aspergillus, being micafungin fungicide for the former and fungistatic ...	2009	19463275
yeast species involved in artisanal cachaça fermentation in three stills with different technological levels in pernambuco, brazil.	both the taste and aroma of cachaça, an alcoholic beverage produced by yeast fermentation of sugar cane, are influenced by yeast metabolites and volatiles. the knowledge of yeast population dynamics during the fermentation process will help to establish the basis for quality control of alcoholic beverage. in the present work, the population dynamics of three fermentation processes, with differing levels of technological sophistication, were studied. saccharomyces cerevisiae was found to be the d ...	2009	19465241
phylogeny and evolution of the aspartyl protease family from clinically relevant candida species.	aspartyl proteases are a class of enzymes that include the yeast aspartyl proteases and secreted aspartyl protease (sap) superfamilies. several sap superfamily members have been demonstrated or suggested as virulence factors in opportunistic pathogens of the genus candida. candida albicans, candida tropicalis, candida dubliniensis and candida parapsilosis harbour 10, four, eight and three sap genes, respectively. in this work, genome mining and phylogenetic analyses revealed the presence of new ...	2009	19547880
identification of high gamma-aminobutyric acid producing marine yeast strains by physiological and biochemical characteristics and gene sequence analyses.	four marine yeasts isolated from the pacific ocean off japan (siki no. 4, siki no. 15, hach no. 6, and inub no. 11), which showed high gamma-aminobutyric acid (gaba) producing abilities, were identified and classified by physiological and biochemical characteristics and gene sequence analyses. analysis of biochemical data suggested that while siki no. 15 was identical to candida, the remaining three isolates belonged to the genus pichia. however, these data were insufficient to resolve their ide ...	2009	19584549
evaluation of chrom-pal medium for the isolation and direct identification of candida dubliniensis in primary cultures from the oral cavity.	candida albicans is the species most frequently isolated from oral specimens, but the recovery of other candida species such as candida dubliniensis is increasing. differentiation of c. dubliniensis from c. albicans requires special tests and both species are misidentified in some studies. chrom-pal (ch-p) is a novel chromogenic medium used in our laboratory for differentiation between c. albicans and c. dubliniensis on the basis of colony colour and morphology, and chlamydospore production. the ...	2009	19608690
candidaemia with uncommon candida species: predisposing factors, outcome, antifungal susceptibility, and implications for management.	the risk factors for and clinical features of bloodstream infection with uncommon candida spp. (species other than c. albicans, c. glabrata, c. parapsilosis, c. tropicals and c. krusei) are incompletely defined. to identify clinical variables associated with these species that might guide management, 57 cases of candidaemia resulting from uncommon candida spp. were analysed in comparison with 517 episodes of candida albicans candidaemia (2001-2004). infection with uncommon candida spp. (5.3% of ...	2009	19614718
effect of phenotypic switching on expression of virulence factors by candida albicans causing candidiasis in diabetic patients.	a total of 110 strains belonging to seven species of candida were isolated from various forms of candidiasis in diabetic patients. they were candida albicans 53 (47%), candida tropicalis 36 (33%), candida glabrata 9 (8%), candida parapsilosis 4 (4%), candida guilliermondii 2 (2%), candida krusei 5 (5%) and candida kefyr 1 (1%). all 53 strains of c. albicans isolated were observed to express virulence factors such as cell surface hydrophobicity (csh), adherence to human buccal epithelial cell (be ...	2009	19635443
[in vitro inhibitory effect of ajoene on candida isolates recovered from vaginal discharges].	the main purpose of this work was to evaluate the in vitro activity of ajoene of the candida, obtained from vaginal discharges.	2009	19635444
heat treatment in combination with antagonistic yeast reduces diseases and elicits the active defense responses in harvested cherry tomato fruit.	this study investigated the effects of heat treatment (hot air at 38 degrees c) and antagonistic yeast (pichia guilliermondii) alone or in combination against postharvest diseases (botrytis cinerea, alternaria alternata and rhizopus nigricans) on cherry tomato fruit, and evaluated the elicitation of active defense responses. results showed that heat treatment at 38 degrees c for 24 h in combination with p. guilliermondii at 1 x 10(8) cfu ml(-1) was the most effective approach to reduce various i ...	2009	19637930
deficiency in frataxin homologue yfh1 in the yeast pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation.	pichia guilliermondii is a representative of yeast species that overproduce riboflavin (vitamin b2) in response to iron deprivation. p. guilliermondii yfh1 gene coding for frataxin homologue, eukaryotic mitochondrial protein involved in iron trafficking and storage, was identified and deleted. constructed p. guilliermondii δyfh1 mutant grew very poorly in a sucrose-containing synthetic medium supplemented with sulfate or sulfite as a sole sulfur source. addition of sodium sulfide, glutathione, c ...	2009	19649569
synergistic anticandidal activity of xanthorrhizol in combination with ketoconazole or amphotericin b.	candida species are responsible for the fourth most common nosocominal bloodstream infection. xanthorrhizol, a sesquiterpene compound isolated from curcuma xanthorrhiza roxb. has been reported to have anticandidal activity. the aim of this study is to investigate the synergistic anticandidal effect of xanthorrhizol in combination with ketoconazole or amphotericin b against candida albicans, candida glabrata, candida guilliermondii, candida krusei, candida parapsilosis, and candida tropicalis. mo ...	2009	19663917
purification of candida guilliermondii and pichia ohmeri killer toxin as an active agent against penicillium expansum.	an antifungal assay with cell-free culture supernatant of pichia ohmeri 158 and candida guilliermondii p3 was tested against penicillium expansum strain #2 at 25 degrees c by measuring hyphal length and percentage conidia germination. c. guilliermondii was more effective against p. expansum conidia germination (58.15% inhibition), while p. ohmeri showed higher inhibition of mycelial growth (66.17%), indicating a probable mechanism associated with killer activity. this killer toxin (molecular mas ...	2009	19680874
polymerase chain reaction assay for specific identification of candida guilliermondii (pichia guilliermondii).	the incidence rates of fungemia caused by candida guilliermondii have been increasing over the past several years. although still relatively rare (1.3% of all cases of fungemia in japan), most cases of c. guilliermondii fungemia occur in patients with cancer or hematological malignancy and their mortality rate is high. as c. guilliermondii tends to be resistant to various antifungal agents, early identification of this pathogen and treatment with an appropriate antifungal agent are required to i ...	2009	19688239
differential response of pichia guilliermondii spoilage isolates to biological and physico-chemical factors prevailing in patagonian wine fermentations.	pichia guilliermondii can produce volatile phenols in the initial stages of wine fermentation; however, its response to different wine stress conditions has been poorly studied. in this work, we present an analysis of the response of 23 p. guilliermondii indigenous isolates to physical and chemical wine stress factors and to indigenous wine killer yeasts. principal coordinates analysis (pcoa), based on data obtained from response patterns, was carried out to evaluate the relationships among the ...	2009	19767852
diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopy.	endophytes are microorganisms that colonize plant tissues internally without causing harm to the host. despite the increasing number of studies on sweet orange pathogens and endophytes, yeast has not been described as a sweet orange endophyte. in the present study, endophytic yeasts were isolated from sweet orange plants and identified by sequencing of internal transcribed spacer (its) rrna. plants sampled from four different sites in the state of são paulo, brazil exhibited different levels of ...	2009	19798655
individual and interaction effects of vanillin and syringaldehyde on the xylitol formation by candida guilliermondii.	the effect of lignin degradation products liberated during chemical hydrolysis of lignocellulosic materials on xylose-to-xylitol bioconversion by candida guilliermondii fti 20037 was studied. two aromatic aldehydes (vanillin and syringaldehyde) were selected as model compounds. a two-level factorial design was employed to evaluate the effects of ph (5.5-7.0), cell concentration (1.0-3.0 g l(-1)), vanillin concentration (0-2.0 g l(-1)) and syringaldehyde concentration (0-2.0 g l(-1)) on this biop ...	2010	19822420
candida neustonensis sp. nov., a novel ascomycetous yeast isolated from the sea surface microlayer in taiwan.	a new ascomycetous yeast species, candida neustonensis is proposed in this study based on four strains (sn92(t), sn47, sj22, sj25) isolated from sea surface microlayer in taiwan. these four yeast strains were morphologically, physiologically and phylogenetically identical to each other. no sexual reproduction was observed on 5% malt extract agar, corn meal agar, v8 agar, mcclary's acetate agar and potato-dextrose agar. phylogenetic analysis of the sequences of the d1/d2 domain of the large subun ...	2010	19823938
spoilage yeasts in patagonian winemaking: molecular and physiological features of pichia guilliermondii indigenous isolates.	yeasts belonging to the genus dekkera/brettanomyces, especially the species dekkera bruxellensis, have long been associated with the production of volatile phenols responsible for off-flavour in wines. according to recent reports, the species pichia guilliermondii could also produce these compounds at the initial stages of fermentation. based on the abundance of p. guilliermondii in patagonian winemaking, we decided to study the relevance of indigenous isolates belonging to this species as wine ...	2009	19831317
identification and characterization of dekkera bruxellensis, candida pararugosa, and pichia guilliermondii isolated from commercial red wines.	yeast isolates from commercial red wines were characterized with regards to tolerances to molecular so(2), ethanol, and temperature as well as synthesis of 4-ethyl-phenol/4-ethyl-guaiacol in grape juice or wine. based on rdna sequencing, nine of the 11 isolates belonged to dekkera bruxellensis (b1a, b1b, b2a, e1, f1a, f3, i1a, n2, and p2) while the other two were candida pararugosa (q2) and pichia guilliermondii (q3). strains b1b, q2, and q3 were much more resistant to molecular so(2) in compari ...	2009	19835781
biodiversity and phytase capacity of yeasts isolated from tanzanian togwa.	the focus of the present investigation was on the tanzanian fermented food togwa as a source for dietary iron and zinc, and the potential for mineral availability improvements using selected yeasts. to establish the content of target minerals and main inhibitor for intestinal uptake, iron and zinc as well as the mineral chelating phytic acid, (ip(6) or phytate) were determined in naturally fermented togwa. yeasts were isolated from sorghum, maize and cassava based togwa, and identified by sequen ...	2010	19906458
study of green sicilian table olive fermentations through microbiological, chemical and sensory analyses.	the production of five different green table olive cultivars was studied by a combined strategy consisting of chemical, microbiological and sensory analyses. cultivable microflora of samples collected during processing was monitored by plate counts on seven synthetic culture media. in all samples enterobacteriaceae, pseudomonaceae, staphylococci, lactic acid bacteria and spore-forming bacteria were undetectable. yeasts and moulds were countable from the day 42 (2 log cfu/ml) till the end of ferm ...	2010	19913708
identification of candida species isolated from bovine mastitic milk and their in vitro hemolytic activity in western turkey.	in this study, identification of 207 candida isolates, previously isolated from mastitic bovine quarter milk samples at the level of genus, was made using api 20 c aux system. the most frequently isolated species were candida krusei (34.8%), followed by candida rugosa (16.4%), candida kefyr (12.6%), candida albicans (10.1%), and candida tropicalis (9.2%). less common isolates were candida zeylanoides (5.8%), candida parapsilosis (4.3%), candida guilliermondii (3.4%), candida famata (1.9%), and c ...	2010	19921462
a combination of heat treatment and pichia guilliermondii prevents cherry tomato spoilage by fungi.	this study investigated the effectiveness of heat treatment and pichia guilliermondii, either alone or in combination, to combat postharvest fungal spoilage in cherry tomato fruit. in vitro experiments demonstrated that heat treatment at 38 degrees c significantly inhibited mycelial growth of three different pathogens (botrytis cinerea, alternaria alternata and rhizopus stolonifer ehrenb). in vivo experiments unveiled that either heat treatment or p. guilliermondii reduced decay caused by these ...	2010	19923029
chemical composition and in vitro antimicrobial activity of the volatile oils from gliomastix murorum and pichia guilliermondii, two endophytic fungi in paris polyphylla var. yunnanensis.	volatile oils were obtained by hydro-distillation from gliomastix murorum and pichia guilliermondii, two endophytic fungi isolated from the traditional chinese medicinal herb paris polyphylla var. yunnanensis. the oils were analyzed for their chemical composition by gas chromatography-mass spectrometry (gc-ms). palmitic acid (15.5%), (e)-9-octadecenoic acid (11.6%), 6-pentyl-5,6-dihydropyran-2-one (9.7%), and (7z,10z)-7,10- hexadecadienoic acid (8.3%) were the major compounds of the 40 identifie ...	2009	19967981
phospholipase and proteinase activities of candida isolates from denture wearers.	summary the aim of the present study was to characterise phospholipase and proteinase activities of oral candida isolates from 100 denture wearers and to study the relationship of these activities with denture stomatitis. of 100 patients studied, 44 suffered from denture stomatitis. specimens were collected by swabbing the denture and underlying mucosa. isolates were previously identified by conventional mycological and genotypic methods. the phospholipase and proteinase activities were evaluate ...	2009	20028461
yeast species associated with wine grapes in china.	having more information on the yeast ecology of grapes is important for wine-makers to produce wine with high quality and typical attributes. china is a significant wine-consuming country and is becoming a serious wine-producer, but little has been reported about the yeast ecology of local ecosystems. this study provides the first step towards the exploitation of the yeast wealth in china's vine-growing regions. the aim of this study was to investigate the yeast population density and diversity ...	2010	20116124
favorable effect of very low initial k(l)a value on xylitol production from xylose by a self-isolated strain of pichia guilliermondii.	xylitol production from xylose by a self-isolated furfural and 5-hydroxymethyl furfural assimilating pichia guilliermondii was studied under oxygen limitation. an extremely low initial volumetric oxygen transfer coefficient (0.075 h(-1)) was found most favorable to the xylitol production with yield of 0.61 g g(-1). related enzymes activities were also investigated and discussed.	2010	20129099
pan-azole-resistant candida guilliermondii from a leukemia patient's silent funguria.	isolation of candida non-albicans yeasts as commensals or pathogens from hospitalised hosts is acquiring increasing importance, due to the frequent drug resistance expressed by such organisms.particularly, the recover of antifungal resistant c. guilliermondii is of worrisome concern, even if recovered as a saprophyte, since commensal yeasts may behave as reservoirs for resistance elements;furthermore, they may enter the bloodstream after chemotherapy-related mucosal damage has developed,thus cau ...	2010	20135354
esophagitis caused by candida guilliermondii in diabetes mellitus: first reported case.	diabetic patients are at risk of acquiring esophageal infections such as those caused by members of the genus candida. here we describe a case in which candida guilliermondii was isolated from the esophageal mucosa of a patient with uncontrolled diabetes mellitus. due to inappropriate and inaccurate identification, the emergence of non-c. albicans candida species as potential pathogens has been underestimated. this should be a cause of concern since c. guilliermondii is a normal component of hum ...	2010	20144130
the chromate resistance phenotype of some yeast mutants correlates with a lower level of cr(v)-species generated in the extra-cellular medium.	the paper describes the selection of chromate-resistant mutants of the yeast pichia guilliermondii with a higher chromate-reducing activity and reports the epr-study of cr(v)-generation in the extra-cellular medium during the reduction of chromate by the yeast culture. it is shown that the reduction of chromate to cr(iii) species runs through the extra-cellular generation of cr(v)-intermediate(s), thus supporting the assumption about the existence of an extra-cellular pathway of cr(vi)-reduction ...	2010	20225069
taxonomic structure of the yeasts and lactic acid bacteria microbiota of pineapple (ananas comosus l. merr.) and use of autochthonous starters for minimally processing.	pichia guilliermondii was the only identified yeast in pineapple fruits. lactobacillus plantarum and lactobacillus rossiae were the main identified species of lactic acid bacteria. typing of lactic acid bacteria differentiated isolates depending on the layers. l. plantarum 1or12 and l. rossiae 2mr10 were selected within the lactic acid bacteria isolates based on the kinetics of growth and acidification. five technological options, including minimal processing, were considered for pineapple: heat ...	2010	20227603
response to oxidative stress in eight pathogenic yeast species of the genus candida.	in the course of an infection, the formation of reactive oxygen species by phagocytes and the antioxidant defense mechanisms of microorganisms play a crucial role in pathogenesis. in this study, isolates representing 8 pathogenic candida species-candida albicans, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida krusei, candida parapsilosis and candida tropicalis-were compared with regard to their resistance to oxidative stress in vitro. we evaluated degree ...	2010	20229037
oral candida carriage of patients attending a dental clinic in braga, portugal.	the ability of the candida species to colonize surfaces can be considered as a risk factor for oral infection.	2010	20403455
cloning, characterization, and engineering of fungal l-arabinitol dehydrogenases.	l-arabinitol 4-dehydrogenase (lad) catalyzes the conversion of l-arabinitol to l-xylulose with concomitant nad(+) reduction in fungal l-arabinose catabolism. it is an important enzyme in the development of recombinant organisms that convert l: -arabinose to fuels and chemicals. here, we report the cloning, characterization, and engineering of four fungal lads from penicillium chrysogenum, pichia guilliermondii, aspergillus niger, and trichoderma longibrachiatum, respectively. the lad from p. gui ...	2010	20414651
genetic and phenotypic diversity of autochthonous cider yeasts in a cellar from asturias.	this paper analyses yeast diversity and dynamics during the production of asturian cider. yeasts were isolated from apple juice and at different stages of fermentation in a cellar in villaviciosa during two asturian cider-apple harvests. the species identified by its-rflp corresponded to hanseniaspora valbyensis, hanseniaspora uvarum, metschnikowia pulcherrima, pichia guilliermondii, candida parapsilosis, saccharomyces cerevisiae and saccharomyces bayanus/saccharomyces pastorianus/saccharomyces ...	2010	20417399
microbiota during fermentation of chum salmon (oncorhynchus keta) sauce mash inoculated with halotolerant microbial starters: analyses using the plate count method and pcr-denaturing gradient gel electrophoresis (dgge).	nine different combinations of mugi koji (barley steamed and molded with aspergillus oryzae) and halotolerant microorganisms (htms), zygosaccharomyces rouxii, candida versatilis, and tetragenococcus halophilus, were inoculated into chum salmon sauce mash under a non-aseptic condition used in industrial fish sauce production and fermented at 35 +/- 2.5 degrees c for 84 days to elucidate the microbial dynamics (i.e., microbial count and microbiota) during fermentation. the viable count of halotole ...	2010	20417400
the dynamics of the yeast community of the tagus river estuary: testing the hypothesis of the multiple origins of estuarine yeasts.	yeasts are common inhabitants of different types of aquatic habitats, including marine and estuarine waters and rivers. although numerous studies have surveyed yeast occurrence in these habitats, the identification of autochthonous populations has been problematic because several yeast species seem to be very versatile and therefore mere presence is not sufficient to establish an ecological association. in the present study we investigated the dynamics of the yeast community in the tagus river e ...	2010	20422287
[susceptibility to fluconazole and voriconazole of candida species isolated from intensive care units patients in medellin, colombia (2001-2007)].	disseminated candidiasis is caused by different candida species and mainly affects immunocompromised patients and those hospitalized in intensive care units (icu).	2010	20450982
analysis of gene evolution and metabolic pathways using the candida gene order browser.	candida species are the most common cause of opportunistic fungal infection worldwide. recent sequencing efforts have provided a wealth of candida genomic data. we have developed the candida gene order browser (cgob), an online tool that aids comparative syntenic analyses of candida species. cgob incorporates all available candida clade genome sequences including two candida albicans isolates (sc5314 and wo-1) and 8 closely related species (candida dubliniensis, candida tropicalis, candida parap ...	2010	20459735