Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| correlation of posaconazole minimum fungicidal concentration and time kill test against nine candida species. | we evaluated the in vitro activity of posaconazole against nine candida species using minimum fungicidal concentration (mfc) measurements and time-kill methods. | 2007 | 17875607 |
| new ethacridine derivatives as the potential antifungal and antibacterial preparations. | until the 20th century fungal infections were rather easy cured, and the need of new antifungal drugs was low. however, low choice of antifungal preparations, their toxicity, limited spectrum of action, and ability to produce resistant strains show the need of new effective medicines for systemic fungal diseases in nowadays. our goal of research was to synthesize new antimicrobial compounds containing three or more pharmacophores in one molecule. the initial 5-substituted-2-methylmercaptothiazol ... | 2007 | 17895642 |
| mixed cultures of serratia marcescens and kluyveromyces fragilis for simultaneous protease production and cod removal of whey. | the aim of this study was to investigate the behaviour of a serratia marcescens-kluyveromyces fragilis mixed culture in whey, with the objective of proteases production and organic waste reduction. | 2007 | 17897188 |
| preparation and kinetic behavior of immobilized whole cell biocatalysts. | actinoplanes missouriensis (for glucose isomerase), kluyveromyces fragilis (for beta-galactosidase), and saccharomyces cerevisiae (for invertase) cells were successfully entrapped within cellulose and cellulose di- and triacetate beads employing several carried solvent systems. cellulose beads prepared using a melt of dimethylsulfoxide (dmso) and n-ethylpyridinium chloride (nepc), or cellulose diacetate using a mixture of acetone and dmso as solvent, were found to be promising as carriers for th ... | 1980 | 17941448 |
| [identification and biodiversity of yeasts isolated from koumiss in xinjiang of china]. | a total of 87 yeast strains were isolated from 28 home-made koumiss samples, a traditional fermented mare milk product in xinjiang of china. the isolates were identified by standard physiological and biochemical tests and analysis of the large-subunit (26s) rdna gene d1/d2 domain sequences. they are proved to be saccharomyces unisporus (48.3% of the isolates), kluyveromyces marxianus (27.6%) and pichia membranaefaciens (15.0%), saccharomyces cerevisiae (9.2%). among them, six isolates and a stan ... | 2007 | 17944353 |
| molecular characterization of kluyveromyces marxianus strains isolated from agave fourcroydes (lem.) in yucatan, mexico. | the molecular characterization of 14 strains of kluyveromyces marxianus isolated from agave fourcroydes (lem.) in yucatan, mexico, was performed by ap-pcr analysis, pcr-rflp of 5.8s-its, and complete nts regions. a sequence analysis of the d1/d2 domain of the 26s rdna was also carried out in six selected strains. the ap-pcr approach had the highest discrimination power for the molecular characterization of new henequen k. marxianus strains. pcr-rflp of 5.8s-its regions did not reveal polymorphis ... | 2007 | 17952662 |
| purification and characterization of the enantioselective esterase from kluyveromyces marxianus cbs 1553. | an intracellular esterase from the yeast kluyveromyces marxianus cbs 1553 with interesting enantioselective hydrolytic activity towards racemic esters of 1,2-o-isopropylidene glycerol (ipg) was purified and characterized. optimal culture conditions for the obtainment of the enantioselective esterase on a 5 l-fermentation scale were investigated. two esterase activities (est1 and est2) in the crude cell extract were identified by native page with specific activity staining and separated from each ... | 2008 | 18029043 |
| characterization of the superoxide dismutase sod1 gene of kluyveromyces marxianus l3 and improved production of sod activity. | superoxide dismutase (sod) activity is one major defense line against oxidative stress for all of the aerobic organisms, and industrial production of this enzyme is highly demanded. the cu/zn superoxide dismutase gene (kmsod1) of kluyveromyces marxianus l3 was cloned and characterized. the deduced kmsod1p protein shares 86% and 71% of identity with kluyveromyces lactis and saccharomyces cerevisiae sod1p, respectively. the characteristic motifs and the amino acid residues involved in coordinating ... | 2008 | 18040680 |
| partial purification and characterization of exoinulinase from kluyveromyces marxianus ys-1 for preparation of high-fructose syrup. | an extracellular exoinulinase (2,1-beta-d fructan fructanohydrolase, ec 3.2.1.7), which catalyzes the hydrolysis of inulin into fructose and glucose, was purified 23.5-fold by ethanol precipitation, followed by sephadex g-100 gel permeation from a cell-free extract of kluyveromyces marxianus ys-1. the partially purified enzyme exhibited considerable activity between ph 5 to 6, with an optimum ph of 5.5, while it remained stable (100%) for 3 h at the optimum temperature of 50 degrees c. mn2+ and ... | 2007 | 18051293 |
| determination of killer activity in yeasts isolated from the elaboration of seasoned green table olives. | in this work 51 yeasts strains isolated from seasoned green table olives and belonging to the candida, debaryomyces, kluyveromyces, pichia, and saccharomyces genera were characterized by their killer activity in different conditions. killer activity of isolates was analyzed in a medium with different ph's (3.5 to 8.5) and nacl concentrations (5, 8, and 10%). at every ph tested, all the genera studied had killer strains, although the smallest percentages of killer yeasts were found at the highest ... | 2008 | 18077043 |
| lactic acid production by mixed cultures of kluyveromyces marxianus, lactobacillus delbrueckii ssp. bulgaricus and lactobacillus helveticus. | lactic acid production using kluyveromyces marxianus (ifo 288), lactobacillus delbrueckii ssp. bulgaricus (atcc 11842) and lactobacillus helveticus (atcc 15009) individually or as mixed culture on cheese whey in stirred or static fermentation conditions was evaluated. lactic acid production, residual sugar and cell biomass were the main features examined. increased lactic acid production was observed, when mixed cultures were used in comparison to individual ones. the highest lactic acid concent ... | 2008 | 18155517 |
| dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. | an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ... | 2008 | 18160452 |
| population diversity of yeasts and lactic acid bacteria in pig feed fermented with whey, wet wheat distillers' grains, or water at different temperatures. | the diversity of populations of yeast and lactic acid bacteria (lab) in pig feeds fermented at 10, 15, or 20 degrees c was characterized by rrna gene sequencing of isolates. the feeds consisted of a cereal grain mix blended with wet wheat distillers' grains (wwdg feed), whey (w feed), or tap water (wat feed). fermentation proceeded for 5 days without disturbance, followed by 14 days of daily simulated feed outtakes, in which 80% of the contents were replaced with fresh feed mixtures. in wwdg fee ... | 2008 | 18223110 |
| production of extracellular exoinulinase from kluyveromyces marxianus ys-1 using root tubers of asparagus officinalis. | root tubers of asparagus officinalis were used as a source of raw inulin for the production of exoinulinase (ec 3.2.1.7) from kluyveromyces marxianus ys-1. root extract prepared at 10kg/cm2 pressure for 10min showed maximum inulinase production. medium components and process parameters were standardized to improve the enzyme production. inulinase yield of 40.2iu/ml in a medium containing raw inulin (3.5%), beef extract (2%), sds (0.001%), mn2+ (2.0mm), mg2+ (1.5mm), co2+ (2mm) and ph 6.5 has bee ... | 2008 | 18280145 |
| development of a stable continuous flow immobilized enzyme reactor for the hydrolysis of inulin. | a 23.5-fold purified exoinulinase with a specific activity of 413 iu/mg and covalently immobilized on duolite a568 has been used for the development of a continuous flow immobilized enzyme reactor for the hydrolysis of inulin. in a packed bed reactor containing 72 iu of exoinulinase from kluyveromyces marxianus ys-1, inulin solution (5%, ph 5.5) with a flow rate of 4 ml/h was completely hydrolyzed at 55 degrees c. the reactor was run continuously for 75 days and its experimental half-life was 72 ... | 2008 | 18389297 |
| synthesis, raman, ft-ir, nmr spectroscopic data and antimicrobial activity of mixed aza-oxo-thia macrocyclic compounds. | mixed aza-oxo-thia macrocyclic ligands 1,3,5,11,13,15-hexaaza-6,10,16,20-tetraoxo-8,18-dithia-2,3,4:12,13,14-dipyridine cyclocosane (l(1)); 1,3,5,12,14,16-hexeaza-6,11,17,22-tetraoxo-8,9,19,20-tetrathia-2,3,4:13,14,15-dipyridine cyclodocosane (l(2)); 1,3,5,13,15,17-hexaaza-6,12,18,24-tetraoxo-9,21-dithia-2,3,4:14,15,16-dipyridine cyclotetracosane (l(3)) and 1,3,5,14,16,18-hexaaza-6,13,19,26-tetraoxo-9,10,22,23-tetrathia-2,3,4:15,16,17-dipyridine cyclohexacosane (l(4)) were synthesised. the struc ... | 2009 | 18417256 |
| yeast biomass production in brewery's spent grains hemicellulosic hydrolyzate. | yeast single-cell protein and yeast extract, in particular, are two products which have many feed, food, pharmaceutical, and biotechnological applications. however, many of these applications are limited by their market price. specifically, the yeast extract requirements for culture media are one of the major technical hurdles to be overcome for the development of low-cost fermentation routes for several top value chemicals in a biorefinery framework. a potential biotechnical solution is the pro ... | 2008 | 18418745 |
| the yeast kluyveromyces marxianus and its biotechnological potential. | strains belonging to the yeast species kluyveromyces marxianus have been isolated from a great variety of habitats, which results in a high metabolic diversity and a substantial degree of intraspecific polymorphism. as a consequence, several different biotechnological applications have been investigated with this yeast: production of enzymes (beta-galactosidase, beta-glucosidase, inulinase, and polygalacturonases, among others), of single-cell protein, of aroma compounds, and of ethanol (includi ... | 2008 | 18427804 |
| hydrolysis of whey lactose using ctab-permeabilized yeast cells. | disposal of lactose in whey and whey permeates is one of the most significant problems with regard to economics and environmental impact faced by the dairy industries. the enzymatic hydrolysis of whey lactose to glucose and galactose by beta-galactosidase constitutes the basis of the most biotechnological processes currently developed to exploit the sugar content of whey. keeping this in view, lactose hydrolysis in whey was performed using ctab permeabilized kluyveromyces marxianus cells. permea ... | 2009 | 18431601 |
| inulinase production in a batch bioreactor using agroindustrial residues as the substrate: experimental data and modeling. | the production of inulinase employing agroindustrial residues as the substrate is a good alternative to reduce production costs and to minimize the environmental impact of disposing these residues in the environment. this study focused on the use of a phenomenological model and an artificial neural network (ann) to simulate the inulinase production during the batch cultivation of the yeast kluyveromyces marxianus nrrl y-7571, employing a medium containing agroindustrial residues such as molasses ... | 2009 | 18449567 |
| amphotericin b-metronidazole combination against candida spp. | oropharyngeal candidiasis (opc) remains a common opportunistic infection in hiv-infected patients. candida albicans is the most frequent causative agent of opc. however, non-albicans spp. are being increasingly isolated. candidal cell wall proteins and mannoproteins play important roles in the biology and patogenesis of candidiasis. in the present study, we have analyzed the proteinaceous components associated with cell wall extracts from c. albicans, candida tropicalis, candida pseudotropicalis ... | 1998 | 18473531 |
| effects of carbon and nitrogen sources and oxygenation on the production of inulinase by kluyveromyces marxianus. | cultivations of kluyveromyces marxianus var. bulgaricus atcc 16045 were performed on both minimal and complex media using different carbon and nitrogen sources either in the presence or absence of aeration. the results collected were worked out and compared so as to provide a useful contribution to the optimization of inulinase production. kinetics of extracellular inulinase release were similar on glucose, fructose, and sucrose. inulinase was detected at basal level since the beginning of batch ... | 2009 | 18483875 |
| identification of yeast and bacteria involved in the mezcal fermentation of agave salmiana. | aims: to identify the yeast and bacteria present in the mezcal fermentation from agave salmiana. methods and results: the restriction and sequence analysis of the amplified region, between 18s and 28s rdna and 16s rdna genes, were used for the identification of yeast and bacteria, respectively. eleven different micro-organisms were identified in the mezcal fermentation. three of them were the following yeast: clavispora lusitaniae, pichia fermentans and kluyveromyces marxianus. the bacteria foun ... | 2008 | 18489025 |
| extraction of inulinase obtained by solid state fermentation of sugarcane bagasse by kluyveromyces marxianus nrrl y-7571. | production of inulinase by solid state fermentation always involves an extraction step, which dictates enzyme recovery yield and is related to cultivation conditions and control of process parameters. this work is focused on the study of extraction conditions aiming to maximize yield of an inulinase obtained by solid state fermentation of sugar cane bagasse and kluyveromyces marxianus nrrl y-7571. kinetics of extraction was followed varying the kind of solvent used. after determining the best so ... | 2008 | 18500581 |
| ethanol production from henequen (agave fourcroydes lem.) juice and molasses by a mixture of two yeasts. | in the fermentation process of henequen (agave fourcroydes lem.) leaf juice, complemented with industrial molasses, the use of an inoculum comprising two yeasts: kluyveromyces marxianus (isolated from the henequen plant) and saccharomyces cerevisiae (commercial strain) was studied. an ethanol production of 5.22+/-1.087% v/v was obtained. contrary to expected, a decrease on ethanol production was observed with the use of the k. marxianus strain. the best results were obtained when a mixture of 25 ... | 2008 | 18524573 |
| ethanol production from jerusalem artichoke tubers (helianthus tuberosus) using kluyveromyces marxianus and saccharomyces rosei. | this article examines the potential of jerusalem artichoke as a source for ethanol and single-cell protein scp. in addition, experimental results are presented on batch fermentation kinetics employing two strains of kluyveromyces marxianus and one strain of saccharomyces rosei grown on the extract derived from the tubers of jerusalem artichoke. of the three cultures examined, kluyveromyces marxianus ucd (fst) 55-82 was found to be the best producer of ethanol grown in a simple medium at 35 degre ... | 1982 | 18546381 |
| simultaneous saccharification and fermentation of kanlow switchgrass pretreated by hydrothermolysis using kluyveromyces marxianus imb4. | a thermotolerant yeast strain named kluyveromyces marxianus imb4 was used in a simultaneous saccharification and fermentation (ssf) process using kanlow switchgrass as a feedstock. switchgrass was pretreated using hydrothermolysis at 200 degrees c for 10 min. after pretreatment, insoluble solids were separated from the liquid prehydrolyzate by filtration and washed with deionized water to remove soluble sugars and inhibitors. insoluble solids were then hydrolyzed using a commercial cellulase pre ... | 2008 | 18546418 |
| continuous ethanol production from jerusalem artichoke tubers. i. use of free cells of kluyveromyces marxianus. | the continuous fermentation of jerusalem artichoke juice to ethanol by free cells of kluyveromyces marxianus ucd (fst) 55-82 has been studied in a continuous-stirred-tank bioreactor at 35 degrees c and ph 4.6. a maximum yield of 90% of the theoretical was obtained at a dilution rate of 0.05 h(-1). about 95% of the sugars were utilized at dilution rates lower than 0.15 h(-1). volumetric ethanol productivity and volumetric biomass productivity reached maximum values of 7 g etoh/l/h and 0.6 g dry w ... | 1982 | 18546449 |
| continuous ethanol production from jerusalem artichoke tubers. ii. use of immobilized cells of kluyveromyces marxianus. | kluyveromyces marxianus ucd (fst) 55-82 cells were immobilized in na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of jerusalem artichoke tubers. volumetric ethanol productivities of 104 and 80 g ethanol/ l/h were obtained at 80 and 92% sugar utilization, respectively. the maximum volumetric ethanol productivity of the immobilized cell bioreactor system was found to be 15 times higher than that of an ordinary-stirred-tank (cst ... | 1982 | 18546450 |
| bioconversion of brewer's spent grains to bioethanol. | spent grains (sg), the residue remaining after extraction of wort, are a major by-product of brewing. this lignocelluose-rich biomass may provide a source of sugars for fuel ethanol fermentations. dilute acid and enzyme treatments were developed to convert the hemicellulose and cellulose fractions to glucose, xylose and arabinose. pretreatment of dried, milled grains with 0.16 n hno(3) at 121 degrees c for 15 min was chosen as the most suitable method for solubilizing grains before enzymatic dig ... | 2008 | 18547331 |
| the ejector-loop fermenter: description and performance of the apparatus. | a novel fermentation unit, the ejector-loop fermenter (elf), consisting of an outer-loop tower fermenter, a centrifugal pump, a plate-heat exchanger, and a gas-liquid ejector, was designed and constructed. aeration was achieved by continuously recirculating the fermentation medium through two different nozzle devices instead of using the traditional expensive air compressor. by carrying out a whey fermentation with kluyveromyces fragilis as the test organism, either in the elf or in conventional ... | 1983 | 18548625 |
| conversion of cheese whey to single-cell protein. | thirteen yeast species belonging to nine genera were screened for the production of single-cell protein (scp) using cheese whey as the substrate. cheese whey supplemented with minerals and yeast extract proved to the best medium for yield, lactose utilization, biomass production, and conversion efficiency. production of beta-galactosidase was studied in brettanomyces anomalus, kluyveromyces fragilis, trichosporon cutaneum, and wingea robertsii; the last proved to be the best strain combining hig ... | 1983 | 18548695 |
| production of biomass and beta-d-galactosidase by candida pseudotropicalis grown in continuous culture on whey. | the production of biomass and beta-d-galactosidase by the lactose-utilizing yeast candida pseudotropicalis ncyc 744 in whey medium was studied. apparent optimization of growth conditions and medium was done in continuous culture. optimal ph and temperature were 2.6 and 36-38 degrees c, respectively, limitations in cu, zn, and possibly mn were detected in deproteinized whey medium. additions of tryptophan stimulated growth of the yeast. under optimal conditions in medium supplemented with excess ... | 1983 | 18548764 |
| temperature profiles of ethanol tolerance: effects of ethanol on the minimum and the maximum temperatures for growth of the yeasts saccharomyces cerevisiae and kluyveromyces fragilis. | 1983 | 18551450 | |
| ethanol production from fodder beets. | various yeasts such as two strains of saccharomyces cerevisiae, saccharomyces diastaticus, and kluyveromyces marxianus were investigated for their ability to ferment fodder beet juice to alcohol. juice extracted from fodder beet roots without any additives was used as a fermentation substrate. the fermentation kinetic parameters were determined and compared for each species of yeast tested. the best species for fodder beet juice fermentation was chosen and products obtained by fermentation of on ... | 1983 | 18551478 |
| ethanol from whey: continuous fermentation with cell recycle. | the production of ethanol from cheese whey lactose has been demonstrated using a single-stage continuous culture fermentation with 100% cell recycle. in a two-step process, an aerobic fed batch operation was used initially to allow biomass buildup in the absence of inhibitory ethanol concentrations. in the anaerobic ethanol-producing second step, a strain of kluyveromyces fragilis selected on the basis of batch fermentation data had a maximum productivity of 7.1 g ethanol/l/h at a dilution rate ... | 1984 | 18551577 |
| enzymatic hydrolysis of inulin to fructose by glutaraldehyde fixed yeast cells. | inulin, a polyfruction, is found as the reserve carbohydrate in the roots and tubers of various plants (i.e. jerusalem artichoke, chicory, and dahlia tubers). the beta-fructofuranosidase (inulase) from the yeast kluyveromyces fragilis is of interest because of its industrial potential in fructose syrup and alcohol production from inulin containing plants. we have found that the inulase of k. fragilis can be immobilized in the yeast cells by glutaraldehyde treatment. these cells are resistant to ... | 1984 | 18553476 |
| optimizing alcohol production from whey using computer technology. | this study was undertaken with the major goal of optimizing the ethanol production from whey using computer technology. to reach this goal, a mathematical model that would describe the fermentation and that could be used for the optimization was developed. kluyveromyces fragilis was the microorganism used to ferment the lactose in the whey into ethanol. preliminary studies showed that k. fragilis produced about 90% of the theoretical ethanol yield when grown in whey-complemented media. however, ... | 1985 | 18553706 |
| comparison between immobilized kluyveromyces fragilis and saccharomyces cerevisiae coimmobilized with beta-galactosidase, with respect to continuous ethanol production from concentrated whey permeate. | kluyveromyces fragilis immobilized in calcium alginate gel was compared to saccharomyces cerevisiae coimmobilized with beta-galactosidase, for continuous ethanol production from whey permeate in packed-bed-type columns. four different whey concentrations were studied, equivalent to 4.5, 10, 15, and 20% lactose, respectively. in all cases the coimmobilized preparation produced more ethanol than k. fragilis. the study went on for more than 5 weeks. k. fragilis showed a decline in activity after 20 ... | 1985 | 18553758 |
| studies on the flocculation characteristics of kluyveromyces marxianus. | 1986 | 18555325 | |
| fuel ethanol and high protein feed from corn and corn-whey mixtures in a farm-scale plant. | distiller's wet grain (dwg) and 95% ethanol were produced from corn in a farm-scale process involving batch cooking-fermentation and continuous distillation-centrifugation. the energy balance was 2.26 and the cost was $1.86/gal (1981 cost). to improve the energy balance and reduce costs, various modifications were made in the plant. the first change, back-end (after liquefaction) serial recycling of stillage supernatant at 20 and 40% strengths, produced beers with 0.2 and 0.4% (v/v) more ethanol ... | 1983 | 18574812 |
| effect of surfactants and zeolites on simultaneous saccharification and fermentation of steam-exploded poplar biomass to ethanol. | in this work, the effect of the addition of different concentrations of tween-80 and three different zeolite-like products on enzymatic hydrolysis, ethanol fermentation, and simultaneous saccharification and fermentation (ssf) process has been investigated. the ability of these products to enhance the effectiveness of the ssf process to ethanol of steam-exploded poplar biomass using the thermotolerant strain kluyveromyces marxianus ems-26 has been tested. tween-80 (0.4 g/l) increased enzymatic h ... | 1998 | 18576005 |
| downstream processing of inulinase. comparison of different techniques. | candida kefyr dsm 70106 was cultivated in a medium containing inulin as a carbon source. about 92% of the inulinase was recovered directly from the medium. different concentration (c(f)) and enrichment (e(f)) factors were obtained, using the following methods: cross-flow filtration (microfiltration and cell diafiltration were carried out using a rotary filter; enzyme ultrafiltration and diafiltration were performed using a cassette module): c(f) = 7.5 and e(f) = 2.2; liquid-liquid extraction of ... | 1998 | 18576016 |
| a mathematical model of ethanol fermentation from cheese whey : i: model development and parameter estimation. | the cybernetic approach to modeling of microbial kinetics in a mixed-substrate environment has been used in this work for the fermentative production of ethanol from cheese whey. in this system, the cells grow on multiple substrates and generate metabolic energy during product formation. this article deals with the development of a mathematical model in which the concept of cell maintenance was modified in light of the specific nature of product formation. continuous culture data for anaerobic p ... | 1997 | 18576106 |
| a mathematical model of ethanol fermentation from cheese whey : ii. simulation and comparison with experimental data. | a cybernetic model for microbial growth on mixed substrates, was used to simulate the anaerobic fermentation of cheese whey and multiple sugars in semisynthetic media by kluyveromyces marxianus cbs 397. the model simulations quite successfully predicted the observed behavior in batch and during transients in continuous operation, in single-substrate systems as well as in media involving multiple substrates, and in semisynthetic and reconstituted cheese whey solutions. the results of simulations ... | 1997 | 18576107 |
| isolation of a respiratory-deficient kluyveromyces fragilis mutant for the production of ethanol from jerusalem artichoke. | a respiratory-deficient, mutant of kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. it was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. under anaerobic conditions this mutant was always better than the wild strain for ethanol production especially from jerusalem artichoke extracts containing large amounts of high polyfructosans ("early" extracts). | 1987 | 18576530 |
| the effect of temperature and ph on ethanol production by free and immobilized cells of kluyveromyces marxianus grown on jerusalem artichoke extract. | the effect of temperature and ph on the kinetics of ethanol production by free and calcium alginate immobilized cells of kluyveromyces marxianus grown on jerusalem artichoke extract was investigated. with the free cells, the ethanol and biomass yields were relatively constant over the temperature range 25-35 degrees c, but dropped sharply beyond 35 degrees c. other kinetic parameters, specific growth rate, specific ethanol production rate, and specific total sugar uptake rate were maximum at 35 ... | 1987 | 18581313 |
| improvements in ethanol tolerance of kluyveromyces fragilis in jerusalem artichoke juice. | alcoholic fermentation of jerusalem artichoke juice, a natural complex medium, allowed the production of 13% (v/v) ethanol utilizing an inulin-fermenting strain of kluyveromyces fragilis, strongly sensitive to ethanol. however, the fermentation of a simple medium with a similar concentration of fermentable sugars (235 g/l) as saccharose stopped prematurely when only 7% (v/v) ethanol had been produced. differences in the two fermentation profiles were attributed to the significantly lower ethanol ... | 1988 | 18584667 |
| the enzymatic hydrolysis and fermentation of pretreated wheat straw to ethanol. | autohydrolysis and ethanol-alkali pulping were used as pretreatment methods of wheat straw for its subsequent saccharification by trichoderma reesei cellulase. the basic hydrolysis parameters, i.e., reaction time, ph, temperature, and enzyme and substrate concentration, were optimized to maximize sugar yields from ethanol-alkali modified straw. thus, a 93% conversion of 2.5% straw material to sugar syrup containing 73% glucose was reached in 48 h using 40 filter paper units/g hydrolyzed substrat ... | 1988 | 18587784 |
| production of exopolysaccharide pullulan from inulin by a mixed culture of aureobasidium pullulans and kluyveromyces fragilis. | 1989 | 18587852 | |
| the use of cellulases from a beta-glucosidase-hyperproducing mutant of trichoderma reesei in simultaneous saccharification and fermentation of wheat straw. | conidia of the cellulolytic strain trichoderma reesei f522 were mutagenized with uv irradiation and n-methyl|-n'-nitro-n-nitrosoguanidine (ntg). a visual agar plate detection system was developed, using esculin and ferric ions, to identify mutants of t. reesei with increased beta-glucosidase activity. selected mutants were tested for production of extracellular cellulases in shake flasks on autohydrolyzed wheat straw as carbon source. the most active mutant v-7 showed about 6-times higher activi ... | 1989 | 18588028 |
| lactase production by solid-state cultivation of kluyveromyces marxianus cdbbl 278 on an inert support: effect of inoculum, buffer, and nitrogen source. | a study was carried out to select the conditions for cultivation of kluyveromyces marxianus cdbbl 278 in solid-state culture (ssc) using polyurethane foam (puf) as an inert support. puf was impregnated with culture media containing lactose (50 g/l) as the carbon and energy source. evaluation of culture parameters during different growth phases was carried out by respirometry. the effect of inoculum level, buffer capacity of the medium, and nitrogen source upon the yield of biomass on lactose (yx ... | 2008 | 18592410 |
| the long-term effects of ethanol on immobilized cell reactor performance using k. fragilis. | the effects of ethanol on reactor performance were studied in a small, 5-cm packed height, "differential" type immobilized cell reactor. lactose utilizing yeast cells, kluyveromyces fragilis, were absorbed to a porous adsorbant sponge matrix in a gas continuous reactor. step changes in the feed ethanol concentration to the column (10-130 g/l) were used to test the reactor response over extended periods of time (about 30-50 h per dosage level) followed by a return to basal zero inlet ethanol feed ... | 1990 | 18595035 |
| solubilization and growth of candida pseudotropicalis in water-in-oil microemulsions. | some new aspects of microbiology in water-in-oil microemulsions are investigated using candida pseudotropicalis in a hexadecane solution containing tween85/span80 (each 5% wt:wt) as surfactant, and limited amount of water (up to 3%, vol:vol), microemulsion solutions containing cells up to 10 mg fresh weight per milliliter can be prepared, which display a greater time stability and a much smaller light scattering than aqueous suspensions having the same cell concentration. this is ascribed to a l ... | 1992 | 18601058 |
| appropriate sampling for intracellular amino acid analysis in five phylogenetically different yeasts. | methanol quenching and fast filtration, the two most common sampling protocols in microbial metabolome analysis, were validated for intracellular amino acid analysis in phylogenetically different yeast strains comprising saccharomyces cerevisiae, kluyveromyces marxianus, pichia pastoris, schizosaccharomyces pombe and zygosaccharomyces bailii. with only few exceptions for selected amino acids, all yeasts exhibited negligible metabolite leakage during quenching with 60% cold buffered methanol. sli ... | 2008 | 18604477 |
| optimization of alkyl beta-d-galactopyranoside synthesis from lactose using commercially available beta-galactosidases. | commercially available lactase (beta-d-galactoside galactohydrolase, ec 3.2.1.23) enzymes produced from kluyveromyces fragilis and kluyveromyces lactis were accessed as catalysts for use in the production of beta-galactopyranosides of various alcohols using lactose as galactosyl donor. the yield of galactoside was enhanced by using the highest practical concentrations of both lactose and alcohol acceptor. the concentrations and thus yield, were limited by the solubility of the substrates. the in ... | 1993 | 18613088 |
| fermentation of cacao (theobroma cacao l.) seeds with a hybrid kluyveromyces marxianus strain improved product quality attributes. | fermentation of theobroma cacao (cacao) seeds is an absolute requirement for the full development of chocolate flavor precursors. an adequate aeration of the fermenting cacao seed mass is a fundamental prerequisite for a satisfactory fermentation. here, we evaluated whether a controlled inoculation of cacao seed fermentation using a kluyveromyces marxianus hybrid yeast strain, with an increased pectinolytic activity, would improve an earlier liquid drainage ('sweatings') from the fermentation ma ... | 2008 | 18616604 |
| highly efficient secretion of heterologous proteins from saccharomyces cerevisiae using inulinase signal peptides. | the inu genes of kluyveromyces marxianus encode inulinases which are readily secreted from saccharomyces cerevisiae into the culture medium. to evaluate the utility of the inu signal peptides for the secretion of heterologous proteins from s. cerevisiae, a variety of expression and secretion vectors were constructed with gal10 promoter and gal7 terminator. the coding sequence for human lipocortin-1 (lc1) was inserted in-frame with the inu signal sequences, and then the secretion efficiency and l ... | 1996 | 18623603 |
| morphological characterization of the dimorphic yeast kluyveromyces marxianus var. marxianus nrrly2415 by semi-automated image analysis. | a semiautomatic image analysis method has been developed to characterize the morphology of the dimorphic yeast kluyveromyces marxianus var. marxianus (formerly fragilis) nrrly2415 undergoing alcoholic fermentation of cheese whey permeate. the method is capable of separating cells into six defined categories, varying from simple ovoid yeast cells to branched mycelial cells. a sample size of 300 cells was found to be sufficient to obtain a statistically significant categorization. the processing t ... | 1996 | 18629834 |
| synthesis, characterization and antimicrobial activity of water soluble dendritic macromolecules. | several families of water soluble dendrimers were synthesized based on poly(propyleneoxide) amines (jeffamines) (p(1)). p(1)-core and branched units were constructed from both methylacrylate and ethylenediamine (p(2)-p(9), and generations 0-3 with -nh(2), -cooh functionalities). they were characterized by elemental analysis (ea), gel permeation chromatography (gpc), ft-ir, (1)h, and (13)c nmr. the antimicrobial activities of only water soluble compounds (p(1), p(3), p(4), p(6), p(7) and p(9)) we ... | 2009 | 18657884 |
| effect of different cellulase dosages on cell viability and ethanol production by kluyveromyces marxianus in ssf processes. | this study was aimed to study the effect of commercial cellulases (celluclast 1.5 lfg) on kluyveromyces marxianus cect 10875 growth and ethanol production in ssf processes. preliminary tests carried out in glucose (50 g/l) fermentation medium showed that high enzyme amounts (2.5-3.5 fpu/ml) could cause a negative effect on k. marxianus growth rate and viable cells number. however, the maximum ethanol production was not affected and about 86% of the theoretical (22 g/l) was reached in all cases i ... | 2009 | 18722114 |
| a traditional sudanese fermented camel's milk product, gariss, as a habitat of streptococcus infantarius subsp. infantarius. | samples of the traditional sudanese fermented camel's milk product gariss representing 9 different regions in sudan were microbiologically characterized using an integrated approach including phenotypic and genotypic methods. lactic acid bacteria [log(cfu/g)=7.76-8.66] and yeasts [log(cfu/g)=6.05-7.79] were detected in high numbers. a total of 180 lab isolates were identified of which 123 isolates were found to belong to the streptococcus bovis group. thirteen representative isolates were charac ... | 2008 | 18774196 |
| cloning and characterization of the inulinase gene from a marine yeast pichia guilliermondii and its expression in pichia pastoris. | the extracellular inulinase structural gene was isolated from the genomic dna of the marine yeast pichia guilliermondii strain 1 by pcr. the gene had an open reading frame of 1,542 bp long encoding an inulinase. the coding region of the gene was not interrupted by any intron. it encoded 514 amino acid residues of a protein with a putative signal peptide of 18 amino acids and the calculated molecular mass of 58.04 kda. the protein sequence deduced from the inulinase structural gene contained the ... | 2009 | 18821076 |
| identification of yeasts and evaluation of their distribution in taiwanese kefir and viili starters. | the objective of the present study was to investigate yeast communities in kefir grains and viili starters in taiwan through conventional microbiological cultivation and polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). the dna sequencing was used as a validity technique to ensure that all isolates within each group belonged to just one species, and to confirm the identified results of pcr-dgge. results indicated that a combination of conventional microbiological cult ... | 2008 | 18832201 |
| automated hplc monitoring of broth components on bioreactors. | under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example hplc or fia) without membrane regeneration. the filtrate stream has been successfully connected to a bioreactor in order to perform the automated hplc analysis of broth components. the monitoring of the carbon source (lactose), and minor products (glyce ... | 1989 | 18925256 |
| high-temperature ethanol fermentation and transformation with linear dna in the thermotolerant yeast kluyveromyces marxianus dmku3-1042. | we demonstrate herein the ability of kluyveromyces marxianus to be an efficient ethanol producer and host for expressing heterologous proteins as an alternative to saccharomyces cerevisiae. growth and ethanol production by strains of k. marxianus and s. cerevisiae were compared under the same conditions. k. marxianus dmku3-1042 was found to be the most suitable strain for high-temperature growth and ethanol production at 45 degrees c. this strain, but not s. cerevisiae, utilized cellobiose, xylo ... | 2008 | 18931291 |
| comparison of enzymatic antioxidant defence systems in different metabolic types of yeasts. | the enzymatic defence system in the 2 yeasts kluyveromyces marxianus and rhodotorula glutinis, differing in their mode of oxygen uptake and energy generation, was characterized and compared with the well-studied facultatively fermentative crabtree-positive saccharomyces cerevisiae strain. twofold higher superoxide dismutase (sod) and catalase activities were detected in k. marxianus and r. glutinis when cells were cultured on glucose. further increases of 10%-15% in sod activity and 30%-50% in c ... | 2008 | 18997852 |
| improved ethanol production by mixed immobilized cells of kluyveromyces marxianus and saccharomyces cerevisiae from cheese whey powder solution fermentation. | ethanol productions from cheese whey powder (cwp) solution were investigated by using free or immobilized cells of kluyveromyces marxianus in monocultures or mixed cultures with free or immobilized cells of k. marxianus and saccharomyces cerevisiae. k. marxianus free cells produced 3.8% v/v ethanol in monocultures, while s. cerevisiae immobilized cells produced 5.3% v/v ethanol in mixed cultures. the percentage of theoretical yield was found to be higher in mixed cultures than that in monocultur ... | 2010 | 19002658 |
| proteinase and phospholipase activity as virulence factors in candida species isolated from blood. | the number of nosocomial blood stream infections due to candida species has increased over the past few decades. in order to establish an infection, opportunistic pathogens have to evade the immune system, survive, divide in the host environment, and spread to other tissues. proteinase and phospholipase secretion has been implicated as potential virulence factors for some candida species responsible for catheter related candidemia in intensive care unit (icu) patients with indwelling devices. we ... | 2008 | 19071887 |
| super life--how and why 'cell selection' leads to the fastest-growing eukaryote. | what is the highest possible replication rate for living organisms? the cellular growth rate is controlled by a variety of processes. therefore, it is unclear which metabolic process or group of processes should be activated to increase growth rate. an organism that is already growing fast may already have optimized through evolution all processes that could be optimized readily, but may be confronted with a more generic limitation. here we introduce a method called 'cell selection' to select fo ... | 2009 | 19087200 |
| ethanol fermentation with kluyveromyces marxianus from jerusalem artichoke grown in salina and irrigated with a mixture of seawater and freshwater. | to study fuel ethanol fermentation with kluyveromyces marxianus atcc8554 from jerusalem artichoke (helianthus tuberosus) grown in salina and irrigated with a mixture of seawater and freshwater. | 2008 | 19120653 |
| crude goat whey fermentation by kluyveromyces marxianus and lactobacillus rhamnosus: contribution to proteolysis and ace inhibitory activity. | unsupplemented acid goat whey containing 0.96% protein and 2.76% lactose was fermented aerobically with 32 microflora extracted from various raw milk cheeses and dairy products. these microflora were screened for their ability to hydrolyse whey proteins (alpha-lactalbumin and/or beta-lactoglobulin) and to generate peptides inhibitors of angiotensin i converting enzyme. five microflora were able to degrade whey protein. the most efficient microflora was able to fully hydrolyse alpha-lactalbumin a ... | 2009 | 19121243 |
| effect of the yeast and bacteria biomass on the microbiota in the rumen. | this study aims at obtaining a probiotic product based on viable biomass from 6 yeast strains and 2 strains of lactic bacteria used for nutrition of animals. the strains are subjected to some resistance tests, at temperature, ph, pepsin, pancreatin and biliary salts so as to make obvious their viability. tests were done by comparison to the witness strain and respectively a protective solution based on mucin and casein. based on the resulted viabilities 2 products are formulated. their effect is ... | 2008 | 19137830 |
| cell disruption optimization and covalent immobilization of beta-d-galactosidase from kluyveromyces marxianus yw-1 for lactose hydrolysis in milk. | beta-d-galactosidase (ec 3.2.1.23) from kluyveromyces marxianus yw-1, an isolate from whey, has been studied in terms of cell disruption to liberate the useful enzyme. the enzyme produced in a bioreactor on a wheat bran medium has been successfully immobilized with a view to developing a commercially usable technology for lactose hydrolysis in the food industry. three chemical and three physical methods of cell disruption were tested and a method of grinding with river sand was found to give hig ... | 2010 | 19198767 |
| whey valorisation: a complete and novel technology development for dairy industry starter culture production. | whey is the major by-product of the dairy industry, produced in large quantities and usually disposed off causing major environmental pollution, due to its high organic load that makes treatment cost prohibitive. this paper comprises a contribution on the valorisation of this high polluting liquid waste of the dairy industry, based on research for the production of novel dairy starter cultures using whey as raw material. starter cultures are used for cheese ripening in order to: (i) accelerate r ... | 2009 | 19254836 |
| [one-step ethanol fermentation with kluyveromyces marxianus yx01 from jerusalem artichoke]. | a unique one-step ethanol fermentation process was developed with the inulinase-producing strain kluyveromyces marxianus yx01. firstly, the impact of temperature on ethanol fermentation was investigated through flask fermentation, and the temperature of 35 degrees c was observed to be the optimum to coordinate inulinase production, inulin saccharification and ethanol fermentation. and then, the impact of aeration and substrate concentration was studied through batch fermentation in the 2.5 l fer ... | 2008 | 19256341 |
| liquid culture of the entomogenous nematode steinernema feltiae with its bacterial symbiont. | the insect-parasitic nematode, steinernema feltiae filipjev strain 42, was reared in liquid culture along with its bacterial symbiont, xenorhabdus nematophilus thomas &poinar. first-stage juveniles developed into reproducing adults in a maintenance salts medium containing resuspended xenorhabdus cells and the yeast kluyveromyces marxianus (hansen) van der walt or cholesterol. cultures with media depths greater than 4 mm required aeration. nematode populations increased as bacterial density incre ... | 1989 | 19287644 |
| use of a sequential strategy of experimental design to optimize the inulinase production in a batch bioreactor. | a potential application of inulinase in the food industry is the production of fructoligosaccharides (fos) by the transfructosilation of sucrose. the foss present many interesting functional properties besides their ability to increase the shelf-life and flavor of many products. the use of an industrial medium represents a good alternative to producing inulinase at low cost, since the activity may improve, or at least remain the same, as that obtained using a synthetic medium. this work was an o ... | 2009 | 19343386 |
| construction of flocculent kluyveromyces marxianus strains suitable for high-temperature ethanol fermentation. | flocculating yeasts are highly useful in fermentation processes because these cells can be separated easily from the fermentation mash. however, native yeasts are usually non-flocculating, including kluyveromyces marxianus, which exhibits a potent high-temperature ethanol fermentation ability. we describe here the construction of flocculent k. marxianus strains via the introduction of the flo1, flo5, flo9, and flo10 genes from saccharomyces cerevisiae. the s. cerevisiae flo genes were overexpres ... | 2009 | 19420680 |
| production of ethanol from the mixture of beet molasses and cheese whey by a 2-deoxyglucose-resistant mutant of kluyveromyces marxianus. | fourteen lactose-fermenting strains of kluyveromyces marxianus, including its anamorph, candida kefyr, were grown in two media containing 20% (w/v) sugar as either beet molasses or cheese whey. strain nbrc 1963 of k. marxianus converted sucrose and lactose to ethanol in both media most efficiently. however, ethanol was produced from sucrose and not from lactose by strain nbrc 1963 in the medium containing equal amounts of sugar from beet molasses and cheese whey. the spontaneous mutants resistan ... | 2009 | 19456875 |
| polygalacturonase secreted by yeasts from brazilian semi-arid environments. | microbial pectinolytic enzymes are known to play a commercially important role in a number of industrial processes. the objective of this study was to investigate the extracellular polygalacturonases of yeasts isolated from brazilian semi-arid environments. among the 250 colonies tested, only 33 produced extracellular polygalacturonases: aureobasidium pullulans (18 isolates), candida boidinii (one isolate), trichosporonoides sp. (three isolates), kluyveromyces marxianus (one isolate), cryptococc ... | 2009 | 19462328 |
| the microbiota of lafun, an african traditional cassava food product. | lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ... | 2009 | 19493582 |
| maximization of beta-galactosidase production: a simultaneous investigation of agitation and aeration effects. | in this work, the agitation and aeration effects in the maximization of the beta-galactosidase production from kluyveromyces marxianus cct 7082 were investigated simultaneously, in relation to the volumetric enzyme activity and the productivity, as well as the analysis of the lactose consumption and production of glucose, and galactose of this process. agitation and aeration effects were studied in a 2 l batch stirred reactor. a central composite design (2(2) trials plus three central points) wa ... | 2010 | 19517069 |
| bioproduction of the aroma compound 2-phenylethanol in a solid-liquid two-phase partitioning bioreactor system by kluyveromyces marxianus. | the rose-like aroma compound 2-phenylethanol (2-pe) is an important fragrance and flavor ingredient. several yeast strains are able to convert l-phenylalanine (l-phe) to 2-pe among which kluyveromyces marxianus has shown promising results. the limitation of this process is the low product concentration and productivity primarily due to end product inhibition. this study explored the possibility and benefits of using a solid-liquid two-phase partition bioreactor (tppb) system as an in situ produc ... | 2009 | 19517523 |
| microbiological and chemical properties of kefir manufactured by entrapped microorganisms isolated from kefir grains. | in this study, various yeasts (kluyveromyces marxianus, saccharomyces turicensis, pichia fermentans) and lactic acid bacteria (lactobacillus kefiranofaciens, lactobacillus kefiri, leuconostoc mesenteroides) were entrapped in 2 different microspheres using an entrapment ratio for the strains that was based on the distribution ratio of these organisms in kefir grains. the purpose of this study was to develop a new technique to produce kefir using immobilized starter cultures isolated from kefir gr ... | 2009 | 19528577 |
| oral distribution of candida species and presence of oral lesions in brazilian leprosy patients under multidrug therapy. | the aim of this study was to evaluate the prevalence of candida spp. and presence of oral lesions in brazilian leprosy patients under multidrug therapy (mdt). | 2009 | 19549111 |
| metals sorption from aqueous solutions by kluyveromyces marxianus: process optimization, equilibrium modeling and chemical characterization. | the dead kluyveromyces marxianus biomass, a fermentation industry waste, was used to explore its sorption potential for lead, mercury, arsenic, cobalt, and cadmium as a function of ph, biosorbent dosage, contact time, agitation speed, and initial metal concentration. the equilibrium data fitted the langmuir model better for cobalt and cadmium, but freundlich isotherm for all metals tested. at equilibrium, the maximum uptake capacity (qmax) was highest for lead followed by mercury, arsenic, cobal ... | 2009 | 19557798 |
| main microorganisms involved in the fermentation of ugandan ghee. | ghee is widely produced from a traditional fermented butter-like product named mashita in western uganda. however, no detailed studies have been done to identify the microorganisms involved in mashita fermentation. the aim of this study was to identify the microorganisms present at the end of mashita ripening using culture-dependent and culture-independent techniques. the most commonly identified species of lactic acid bacteria (lab) in mashita using culture-dependent techniques were lactobacill ... | 2009 | 19577815 |
| evaluation of chrom-pal medium for the isolation and direct identification of candida dubliniensis in primary cultures from the oral cavity. | candida albicans is the species most frequently isolated from oral specimens, but the recovery of other candida species such as candida dubliniensis is increasing. differentiation of c. dubliniensis from c. albicans requires special tests and both species are misidentified in some studies. chrom-pal (ch-p) is a novel chromogenic medium used in our laboratory for differentiation between c. albicans and c. dubliniensis on the basis of colony colour and morphology, and chlamydospore production. the ... | 2009 | 19608690 |
| effect of phenotypic switching on expression of virulence factors by candida albicans causing candidiasis in diabetic patients. | a total of 110 strains belonging to seven species of candida were isolated from various forms of candidiasis in diabetic patients. they were candida albicans 53 (47%), candida tropicalis 36 (33%), candida glabrata 9 (8%), candida parapsilosis 4 (4%), candida guilliermondii 2 (2%), candida krusei 5 (5%) and candida kefyr 1 (1%). all 53 strains of c. albicans isolated were observed to express virulence factors such as cell surface hydrophobicity (csh), adherence to human buccal epithelial cell (be ... | 2009 | 19635443 |
| goat whey fermentation by kluyveromyces marxianus and lactobacillus rhamnosus release tryptophan and tryptophan-lactokinin from a cryptic zone of alpha-lactalbumin. | angiotensin-i-converting enzyme (ace) inhibitors peptides were produced from unsupplemented acid goat whey fermented aerobically for 168 h by kluyveromyces marxianus and lactobacillus rhamnosus. this yeast-lactobacillus association is gras. two novel lactokinins were identified: nyw and w with ic50 of 20 and 0.86 mum respectively. they both were resistant toward simulated gastrointestinal digestion. in addition, wlahk was found in the hydrolysate. these three sequences belong to f(99-110) of alp ... | 2009 | 19650957 |
| enhancement of survival of probiotic and non-probiotic lactic acid bacteria by yeasts in fermented milk under non-refrigerated conditions. | the effects of yeasts on the survival of probiotic and non-probiotic lactic acid bacteria (lab) were studied in fermented milk under non-refrigerated conditions (30 degrees c) with a view to develop ambient-stable fermented milk with live lab. five yeasts tested (saccharomyces bayanus, williopsis saturnus var. saturnus, yarrowia lipolytica, candida kefyr and kluyveromyces marxianus) enhanced the survival of lactobacillus bulgaricus (but not streptococcus thermophilus) in a mixed yoghurt culture ... | 2009 | 19666198 |
| side-chain structure of cell surface polysaccharide, mannan, affects hypocholesterolemic activity of yeast. | we previously reported that kluyveromyces marxianus yit 8292 exhibited more potent hypocholesterolemic activity than other yeasts containing saccharomyces cerevisiae . to clarify the reason for the higher hypocholesterolemic activity, we examined the side-chain structure of cell surface polysaccharide, mannan, of k. marxianus yit 8292. the result shows that k. marxianus yit 8292 had shorter alpha-(1,2)-linked oligomannosyl side chains and lower phosphate content in mannan than s. cerevisiae. the ... | 2009 | 19670864 |
| molecular identification and susceptibility profile in vitro of the emerging pathogen candida kefyr. | candida kefyr has been increasingly associated with bloodstream infections. this study reports on the identification and antifungal susceptibility pattern of 33 candida isolates, identified biochemically as c. kefyr. one strain was finally identified as candida sphaerica (kluyveromyces lactis) by its sequencing. both species seem to be emerging pathogens and highly susceptible in vitro to currently licensed antifungal compounds. | 2010 | 19709840 |
| cloning, characterization, and functional analysis of the epg1-2 gene: a new allele coding for an endopolygalacturonase in kluyveromyces marxianus. | a new allele, the epg1-2 gene, which codes for an endopolygalacturonase in kluyveromyces marxianus cect1043, has been cloned. the gene has 1086 bp and the protein 362 amino acids, one more than the previously described epg1p. epg1-2p shows a high degree of similarity with the polygalacturonases of fungi and yeasts. the sequences common to all of the polygalacturonases of prokaryotes, fungi, and higher plants are also conserved in epg1-2p. the epg1-2 gene has been expressed in pichia pastoris , a ... | 2009 | 19725536 |
| purification and partial characterization of cu/zn superoxide dismutase from kluyveromyces marxianus yeast. | a new thermostable cu/zn sod from a thermotolerant yeast strain kluyveromyces marxianus nbimcc 1984 has been purified and characterized. the purification procedure comprises thermal treatment and dialysis, ion-exchange chromatography and chromatofocusing. the methodology is a rapid, efficient and highly specific, generating pure preparation (specific activity 996 u mg of protein(-1)) with a yield of 53%. the purified enzyme is a homodimer with mw of 34,034 da and has high n-terminal homology wit ... | 2009 | 19758847 |
| a novel acid-stable, acid-active beta-galactosidase potentially suited to the alleviation of lactose intolerance. | extracellular beta-galactosidase produced by a strain of aspergillus niger van tiegh was purified to homogeneity using a combination of gel filtration, ion-exchange, chromatofocusing, and hydrophobic interaction chromatographies. the enzyme displayed a temperature optimum of 65 degrees c and a low ph optimum of between 2.0 and 4.0. the monomeric glycosylated enzyme displayed a molecular mass of 129 kda and an isoelectric point of 4.7. protein database similarity searching using mass spectrometry ... | 2010 | 19806354 |
| high-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast? | the process of ethanol fermentation has a long history in the production of alcoholic drinks, but much larger scale production of ethanol is now required to enable its use as a substituent of gasoline fuels at 3%, 10%, or 85% (referred to as e3, e10, and e85, respectively). compared with fossil fuels, the production costs are a major issue for the production of fuel ethanol. there are a number of possible approaches to delivering cost-effective fuel ethanol production from different biomass sour ... | 2010 | 19820925 |
| strategy for biotechnological process design applied to the enzymatic hydrolysis of agave fructo-oligosaccharides to obtain fructose-rich syrups. | a strategy to optimize biotechnological process design is illustrated for the production of fructose-rich syrups via enzymatic hydrolysis of agave fructo-oligosaccharides. the optimization process includes ecological studies from natural fermentations leading to the selection of a strain with capacity for inulinase synthesis, and variable optimization for the synthesis, and enzymatic hydrolysis using the response surface methodology. the results lead to the selection of kluyveromyces marxianus , ... | 2009 | 19827764 |
| analysis of the microflora in tibetan kefir grains using denaturing gradient gel electrophoresis. | the microflora of tibetan kefir grains was investigated by culture- independent methods. denaturing gradient gel electrophoresis (dgge) of partially amplified 16s rrna for bacteria and 26s rrna for yeasts, followed by sequencing of the most intense bands, showed that the dominant microorganisms were pseudomonas sp., leuconostoc mesenteroides, lactobacillus helveticus, lactobacillus kefiranofaciens, lactococcus lactis, lactobacillus kefiri, lactobacillus casei, kazachstania unispora, kluyveromyce ... | 2009 | 19835760 |