Publications

purification by immunoadsorption and immunochemical properties of nadp-dependent malic enzymes from leaves of c(3), c(4), and crassulacean acid metabolism plants.	nadp:malic enzyme from corn (zea mays l.) leaves was purified by conventional techniques to apparent homogeneity as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antibodies raised against this protein in rabbits were purified, coupled covalently to protein a-sepharose cl-4b, and used as an immunoaffinity resin to purify the nadp:malic enzymes of the c(3) plants spinach (spinacia oleracea l.) and wheat (triticum aestivum l.), of the crassulacean acid metabolism (cam) plant ...	1990	16667339
diazotrophic burkholderia species associated with field-grown maize and sugarcane.	until recently, diazotrophy was known in only one of the 30 formally described species of burkholderia. novel n(2)-fixing plant-associated burkholderia species such as b. unamae, b. tropica, and b. xenovorans have been described, but their environmental distribution is scarcely known. in the present study, the occurrence of n(2)-fixing burkholderia species associated with different varieties of sugarcane and maize growing in regions of mexico and brazil was analyzed. only 111 out of more than 90 ...	2006	16672447
microbiological evaluation of sugarcane juice sold at street stands and juice handling conditions in são carlos, são paulo, brazil.	fresh sugarcane juice is sold by street vendors without any heat treatment in são carlos, são paulo, brazil. twenty-four samples of point-of-sale juice were tested by standard methods to determine heterotrophic bacteria, total and thermo-tolerant coliform counts, salmonella, and parasites in the juice. 25% of samples showed poor sanitary conditions, with thermo-tolerant coliform levels higher than allowed by brazilian standards. salmonella spp. and parasites were absent in all samples. thermo-to ...	2006	16680364
expression and variability of molecular chaperones in the sugarcane expressome.	molecular chaperones perform folding assistance in newly synthesized polypeptides preventing aggregation processes, recovering proteins from aggregates, among other important cellular functions. thus their study presents great biotechnological importance. the present work discusses the mining for chaperone-related sequences within the sugarcane est genome project database, which resulted in approximately 300 different sequences. since molecular chaperones are highly conserved in most organisms s ...	2007	16687190
distribution of anopheles albimanus, anopheles vestitipennis, and anopheles crucians associated with land use in northern belize.	anthropogenic land use changes often alter natural patterns of disease transmission. the goal of this study was to determine whether phosphorus input from sugarcane, saccharum officinarum l., cultivation in northern belize could pose a significant environmental impact on malaria transmission by changing vegetation structure and composition of wetlands and associated larval habitats. our primary focus was on the increased dominance of cattail, typha domingensis pers., a favored habitat for anophe ...	2006	16739424
n-fertilizer saving by the inoculation of gluconacetobacter diazotrophicus and herbaspirillum sp. in micropropagated sugarcane plants.	colonization of micropropagated sugarcane plants by gluconacetobacter diazotrophicus and herbaspirillum sp. was confirmed by a dot-immunoblot assay. in all, a 45-day short-term and 180-day long-term experiments conducted on micropropagated sugarcane plants of co 86032, a sugar rich popular variety in south india, indicated the usefulness of these diazotrophs as plant growth promoting bacteria. co-inoculation of these two bacteria enhanced the biomass considerably under n-limited condition in the ...	2006	16765840
enhanced xylitol production by precultivation of candida guilliermondii cells in sugarcane bagasse hemicellulosic hydrolysate.	the present work evaluated the key enzymes involved in xylitol production (xylose reductase [xr] and xylitol dehydrogenase [xdh]) and their correlation with xylose, arabinose, and acetic acid assimilation during cultivation of candida guilliermondii fti 20037 cells in sugarcane bagasse hemicellulosic hydrolysate. for this purpose, inocula previously grown either in sugarcane bagasse hemicellulosic hydrolysate (sbhh) or in semidefined medium (xylose as a substrate) were used. the highest xylose/a ...	2006	16775788
plant growth inhibitors isolated from sugarcane (saccharum officinarum) straw.	several compounds related with plant defense and pharmacological activities have been isolated from sugarcane. straw phytotoxins and their possible mechanisms of growth inhibition are largely unknown. a bioassay-guided fractionation of the phytotoxic constituents leachated from a sugarcane straw led to the isolation of trans-ferulic (trans-fa), cis-ferulic (cis-fa), vanillic (va) and syringic (sa) acids. the straw leachates and their identified constituents significantly inhibited root growth of ...	2006	16777531
laboratory and field tests of spray-dried and granular formulations of a bacillus thuringiensis strain with insecticidal activity against the sugarcane borer.	formulations of bacillus thuringiensis berliner (bt) with insecticidal activity against the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: pyralidae), were developed and tested under laboratory and field conditions. the formulations were prepared using biodegradable polymers such as modified corn starch as an encapsulating agent, gelatin as an adherent, powdered sugarcane as a feeding stimulant and a bt var. kurstaki gm-34 strain from a non-sugarcane region as the active ingredien ...	2006	16786544
captures of boll weevils (coleoptera: curculionidae) in traps associated with different habitats.	programs to eradicate the boll weevil, anthonomus grandis grandis boheman, from cotton, gossypium hirsutum l., in the united states rely heavily on pheromone traps for monitoring weevil populations in both active and posteradication maintenance programs. modifications to trapping protocols that increase trap effectiveness should contribute to this eradication effort. between october 1996 and may 1997 and between september 1997 and april 1998, we compared trap effectiveness, indicated by the numb ...	2006	16813308
sugarcane borer (lepidoptera: crambidae) management threshold assessment on four sugarcane cultivars.	this research assesses the potential for using different economic injury thresholds in management of a key insect pest on susceptible and resistant commercially produced cultivars of sugarcane (saccharum spp. hybrids). in a 2-yr sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), study involving four commercially produced sugarcane cultivars and four insecticide treatment thresholds, 'lcp 85-384' and 'hocp 91-555' were the most susceptible based on percentage of bored internodes ...	2006	16813338
use of complex media for the production of scleroglucan by sclerotium rolfsii mtcc 2156.	submerged fermentation was carried out for the production of scleroglucan by sclerotium rolfsii mtcc 2156 using complex media, such as coconut water, sugarcane molasses and sugarcane juice at 28+/-2 degrees c and 180 rpm for 72 h. sugarcane juice gave maximum scleroglucan production of 23.87 g/l as compared to 12.58 and 18.45 g/l with coconut water and sugarcane molasses, respectively. utilization of these substrates would be ecologically sound and economically advantageous.	2007	16822667
sugarcane molasses and yeast powder used in the fructooligosaccharides production by aspergillus japonicus-fcl 119t and aspergillus niger atcc 20611.	different concentrations of sucrose (3-25% w/v) and peptone (2-5% w/v) were studied in the formulation of media during the cultivation of aspergillus japonicus-fcl 119t and aspergillus niger atcc 20611. moreover, cane molasses (3.5-17.5% w/v total sugar) and yeast powder (1.5-5% w/v) were used as alternative nutrients for both strains' cultivation. these media were formulated for analysis of cellular growth, beta-fructosyltransferase and fructooligosaccharides (fos) production. transfructosylati ...	2006	16835781
production of cellulase-free endoxylanase from novel alkalophilic thermotolerent bacillus pumilus by solid-state fermentation and its application in wastepaper recycling.	the present study aimed at optimization of culture condition for the enhanced production of extra cellular thermostable cellulase-free xylanase from bacillus pumilus by solid-state fermentation. batch studies were carried out to evaluate various agro-industrial residues such as rice bran, rice husk, rice straw, sawdust, coconut pith, sugarcane bagasse and wheat bran for enzyme production by the bacterial culture. the endoxylanase production was highest on wheat bran media (5582 u/gds), which was ...	2007	16844369
assessment of textile dye remediation using biotic and abiotic agents.	the aim of the current work was to assess the removal of direct and reactive dyes using biotic and abiotic agents. removal of dyes and their derivatives from aqueous solutions was investigated using sugarcane bagasse, sawdust, rice straw, charcoal and fungal biomass as dye removing agents. seven fungal strains known to have high capacity in removing textile dyes were used. results of this study indicated that penicillium commune, p. freii, and p. allii removed 96, 64 and 65%, respectively, of di ...	2006	16847836
purification and characterization of a maltooligosaccharide-forming alpha-amylase from a new bacillus subtilis kcc103.	a maltooligosaccharide-forming alpha-amylase was produced by a new soil isolate bacillus subtilis kcc103. in contrast to other bacillus species, the synthesis of alpha-amylase in kcc103 was not catabolite-repressed. the alpha-amylase was purified in one step using anion exchange chromatography after concentration of crude enzyme by acetone precipitation. the purified alpha-amylase had a molecular mass of 53 kda. it was highly active over a broad ph range from 5 to 7 and stable in a wide ph range ...	2006	16850297
isolation and characterization of cellulose obtained from ultrasonic irradiated sugarcane bagasse.	cell walls of sugarcane bagasse were first delignified with chlorite followed by ultrasonic irradiation and then by two-step sequential extractions at 23 degrees c with 15 and 18% koh for 2 h, 15 and 18% naoh for 2 h, 8 and 10% koh for 12 h, and 8 and 10% naoh for 12 h and by a single one-stage isolation with 10% koh for 16 h and with 10% naoh for 16 h, which released 79.4, 81.8, 83.6, 85.7, 61.5, and 65.6% of the original hemicelluloses, and subsequently yielded 50.7, 49.5, 48.6, 47.8, 57.2, an ...	2006	16881672
genetic segregation of microsatellite markers in saccharum officinarum and s. spontaneum.	genetic mapping techniques can be used to study the interaction between two different genomes after hybridization. this study investigated a saccharum officinarum (green german or gg, 2n approximately 11x approximately 110) x s. spontaneum (ind 81-146 or ind, 2n approximately 7x approximately 56) interspecific cross. segregation of 193 microsatellite (ssr) loci was evaluated in the f(1) progeny of 169 full-sibs of the cross. following the two-way pseudo-testcross strategy and 'cross pollination' ...	2006	16912699
effects of genetic polymorphisms cyp1a1, gstm1, gstt1 and gstp1 on urinary 1-hydroxypyrene levels in sugarcane workers.	sugarcane workers in brazil are exposed to various genotoxic compounds, including polycyclic aromatic hydrocarbons (pahs), derived from an incomplete combustion process of burnt sugarcane fields. the effects of the occupational exposure to sugarcane fields burning were measured in urine samples of sugarcane workers from the northwest of the state of são paulo when exposed (harvesting) and when non-exposed (non-harvesting). the urinary levels of 1-hydroxypyrene (1-ohp) and the influence of the ge ...	2006	16914185
inulinase production by kluyveromyces marxianus nrrl y-7571 using solid state fermentation.	inulinase is an enzyme relevant to fructose production by enzymatic hydrolysis of inulin. this enzyme is also applied in the production of fructo-oligosaccharides that may be used as a new food functional ingredient. commercial inulinase is currently obtained using inulin as substrate, which is a relatively expensive raw material. in brazil, the production of this enzyme using residues of sugarcane and corn industry (sugarcane bagasse, molasses, and corn steep liquor) is economically attractive, ...	2006	16915703
adaptation of a recombinant xylose-utilizing saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors.	adaptation of a xylose-utilizing genetically engineered strain of saccharomyces cerevisiae to sugarcane bagasse hydrolysates by cultivation during 353h using medium with increasing concentrations of inhibitors, including phenolic compounds, furaldehydes and aliphatic acids, led to improved performance with respect to ethanol production. the remaining xylose concentration in the medium at the end of the cultivation was 5.2g l(-1), while it was 11gl(-1) in the feed, indicating that approximately h ...	2007	16934451
use of post-harvest sugarcane residue for ethanol production.	agricultural residues are produced in large quantities throughout the world. approximately, 1kg of residue is produced for each kilogram of grains harvested. this ratio of grain/residue translates into an excess of 40 billion ton of crop residue produced each year in the usa. these residues are renewable resources that could be used to produce ethanol and many other value added products. in this study, we demonstrate that the post-harvest sugar cane residue could be used to produce fuel grade et ...	2007	16935500
short-range dispersal and overwintering habitats of boll weevils (coleoptera: curculionidae) during and after harvest in the subtropics.	field experiments in the subtropical lower rio grande valley of texas were conducted to determine the extent of adult boll weevil, anthonomus grandis grandis boheman (coleoptera: curculionidae), dispersal from cotton, gossypium hirsutum l., fields during harvest operations and the noncotton-growing ("overwinter") period between 1 september and 1 february. using unbaited large capacity boll weevil traps placed at intervals extending outward from commercial field edges, boll weevils did not move i ...	2006	16937667
pairwise coupling analysis of helical junction hydrogen bonding interactions in luteoviral rna pseudoknots.	a 28-nucleotide mrna pseudoknot that overlaps the p1 and p2 genes of sugarcane yellow leaf virus (scylv) stimulates -1 ribosomal frameshifting. the in vitro frameshifting efficiency is decreased >or=8-fold upon substitution of the 3'-most loop 2 nucleotide (c27) with adenosine, which accepts a hydrogen bond from the 2'-oh group of c14 in stem s1. the solution structures of the wild-type (wt) and c27a scylv rna pseudoknots show that while the rnas adopt virtually identical overall structures, the ...	2006	16964977
female reproductive system of the sugarcane spittlebug mahanarva fimbriolata (auchenorrhyncha): vitellogenesis dynamics and protein quantification.	the present study aimed describing the ovaries of the sugarcane spittlebug mahanarva fimbriolata which are meroistic telotrophic with nurse cells and oocytes located in the tropharium. sem revealed paired ovaries located dorsolaterally around the intestine, and oocytes exhibiting shapes ranging from round (less developed) to elliptic (more developed), suggesting a simultaneous, although, asynchronous development. based on histological data we classified the oocytes in stages from i to v. stage i ...	2007	16973368
evidence for the dispersal of a unique lineage from asia to america and africa in the sugarcane fungal pathogen ustilago scitaminea.	the basidiomycete ustilago scitaminea sydow, which causes sugarcane smut disease, has been spreading throughout africa and america since the 1940s. the genetic diversity and structure of different populations of this fungus worldwide was investigated using microsatellites. a total of 142 single-teliospore were isolated from 77 distinct whips (sori) collected in 15 countries worldwide. mycelium culture derived from on generation of selfing of these single teliospores were analysed for their polym ...	2007	16979360
the global structures of a wild-type and poorly functional plant luteoviral mrna pseudoknot are essentially identical.	the helical junction region of a -1 frameshift stimulating hairpin-type mrna pseudoknot from sugarcane yellow leaf virus (scylv) is characterized by a novel c27.(g7-c14) loop 2-stem 1 minor groove base triple, which is stacked on a c8+.(g12-c28) loop 1-stem 2 major groove base triple. substitution of c27 with adenosine reduces frameshifting efficiency to a level just twofold above the slip-site alone. here, we show that the global structure of the c27a scylv rna is nearly indistinguishable from ...	2006	17000902
heavy metal concentrations in soils and plant accumulation in a restored manganese mineland in guangxi, south china.	heavy metal contamination of metal-mined soils is a widespread problem in china. in the restored (over 20 years) lipu manganese mineland, 36 plant species from 22 families were found colonizing, some of which were planted agronomic ones. heavy metal concentrations in tailings were very high. minesoils were basically unpolluted, but soils in the remaining mining area and in the vicinity of tailings dumps were polluted by cd and mn. measurements of metal contents in dominant plants showed they wer ...	2007	17014941
on-line identification of further flavone c- and o-glycosides from sugarcane (saccharum officinarum l., gramineae) by hplc-uv-ms.	hplc-uv-ms (apci-ms/ms and cid/ms) was utilised for the identification of eight additional flavone glycosides from sugarcane (saccharum officinarum l., gramineae) extracts of bagasse, leaves and juice ('garapa'). relevant information about substitution patterns was obtained through uv detection using post-column addition of shift reagents, while tandem ms provided structural information confirming the proposed structures of the c-glycosides vitexin, orientin, luteolin-8-c-(rhamnosylglucoside), 4 ...	2012	17019935
physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane (saccharum officinarum) sprouts.	global increase in ambient temperature is a critical factor for plant growth. in order to study the changes in growth over short intervals, various primary and secondary metabolites, and their relationships with thermotolerance, 1-month-old sugarcane (saccharum officinarum) sprouts were grown under control conditions (28 degrees c) or under heat-stress conditions (40 degrees c), and measurements were made at six 12-h intervals. heat stress greatly reduced dry matter and leaf area of sprouts init ...	2007	17024517
mapping of risk prone areas of kala-azar (visceral leishmaniasis) in parts of bihar state, india: an rs and gis approach.	the kala-azar fever (visceral leishmaniasis) is continuing unabated in india for over a century, now being largely confined to the eastern part of india mainly in bihar state and to some extent in its bordering states like west bengal and uttar pradesh. two study sites namely patepur block in vaishali district with high endemicity in northern part and lohardagga block in lohardagga district with absolute non-endemicity in southern part of bihar were selected for the study with the following obje ...	2006	17024860
sugarcane (saccharum spp.).	we describe the procedures for recovering transgenic sugarcane from co-cultivation of both calli and in vitro plants with agrobacterium tumefaciens. the correct tissue culture strategies and the use of super-binary vector or super-virulent strain are crucial for the successful sugarcane transformation. both plant regeneration via calli culture and micropropagation strategies can be optimized to a wide spectrum of sugarcane genotypes, thus the procedures presented here could be applied to genetic ...	2006	17033066
[screening of microorganism producing polyunsaturated fatty acids with sugarcane molasses].	a strain name lb1 producing polyunsaturated fatty acids with sugarcane molasses was screened form soil samples by the methods of sudan black b stain and the determination of pufas' iodine value. based on the grass morphological and physiological characteristics and the sequence similarity of ribosomal dna-its, the strain was identified as mucor recurvus sp.. the results of l64 (4(3)) orthogonal experiments indicated when mucor recurvus was cultivated at 28 degrees c for 5 days with 160 r/min, th ...	2006	17037053
associations between dna markers and resistance to diseases in sugarcane and effects of population substructure.	association between markers and sugarcane diseases were investigated in a collection of 154 sugarcane clones, consisting of important ancestors or parents, and cultivars. 1,068 polymorphic aflp and 141 srr markers were scored across all clones. data on the four most important diseases in the australian sugarcane industry were obtained; these diseases being pachymetra root rot (pachymetra chaunorhiza b.j. croft & m.w. dick), leaf scald (xanthomonas albilineans dowson), fiji leaf gall (fiji diseas ...	2006	17047910
mutagenesis and analysis of mold aspergillus niger for extracellular glucose oxidase production using sugarcane molasses.	aspergillus niger ors-4.410, a mutant of a. niger ors-4, was generated by repeated ultraviolet (uv) irradiation. analysis of the uv treatment dose on wild-type (wt) a. niger ors-4, conidial survival, and frequency of mutation showed that the maximum frequency of positive mutants (25.5%) was obtained with a 57% conidial survival rate after the second stage of uv irradiation. the level of glucose oxidase (gox) production from mutant a. niger ors-4.410 thus obtained was 149% higher than that for wt ...	2006	17057255
transmission of sugarcane white leaf phytoplasma by yamatotettix flavovittatus, a new leafhopper vector.	sugarcane white leaf disease is caused by plant pathogenic phytoplasmas that are transmitted to the plant by the leafhopper matsumuratettix hiroglyphicus (matsumura). to determine whether there are other insect vectors that transmit this disease pathogen, leafhopper species in sugarcane, saccharum officinarum l., fields in northeastern thailand were monitored by using light traps. sixty-nine leafhopper species from family cicadellidae were found. using nested polymerase chain reaction (pcr) with ...	2006	17066780
resistance to stem borers (lepidoptera: crambidae) among texas rice cultivars.	a 4-yr field study was conducted to assess the resistance of rice, oryza sativa l., cultivars to injury from the sugarcane borer, diatraea saccharalis (f.), and the mexican rice borer, eoreuma loftini (dyar) (both lepidoptera: crambidae). several cultivars showed reduced levels of injury ('clxl8', 'xl8', 'wells', 'cheniere', and 'xp710' in 2003; clxl8, xp723, cheniere, and 'cl161' in 2004) and lower stem borer yield loss (clxl8 in 2004) than the more susceptible 'priscilla'. the resistant clxl8 ...	2006	17066824
efficient isolation of anthraquinone-derivatives from trichoderma harzianum ets 323.	anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from trichoderma harzianum ets 323. the fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. structure of chrysophanol was solved by x-ray diffraction and pachybasin by nmr spectra. about 233+/-13 mg of pure chrysophanol and 773+/-40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7+/ ...	2007	17067682
the effect of land use on soil health indicators in peri-urban agriculture in the humid forest zone of southern cameroon.	the objective of this study was to identify the effect of different land uses in peri-urban agriculture on the soil properties. soil health indicators were evaluated in the top 10 cm at five tilled agricultural sites involving different cropping systems and use of agrochemicals within the peri-urban agricultural areas of yaounde, cameroon, and compared with a native forest land. the experimental data showed that the selected indicators were sensitive to cropping practice. most cropped land had s ...	2006	17071911
status of eldana saccharina (lepidoptera: pyralidae), its host plants and natural enemies in ethiopia.	surveys for sugarcane stem borers were undertaken in ethiopia to determine the prevalence and distribution of these and their natural enemies in crops and indigenous host plants. eldana saccharina walker was not recovered from sugarcane, but was present in three indigenous wetland sedges, cyperus papyrus, c. fastigiatus and c. dives in the southern, central and northern part of the country. the latter indigenous host plant was present in waterways adjacent to sugarcane on the commercial sugar es ...	2006	17092361
arabidopsis sucrose transporter atsuc9. high-affinity transport activity, intragenic control of expression, and early flowering mutant phenotype.	atsuc9 (at5g06170), a sucrose (suc) transporter from arabidopsis (arabidopsis thaliana) l. heynh., was expressed in xenopus (xenopus laevis) oocytes, and transport activity was analyzed. compared to all other suc transporters, atsuc9 had an ultrahigh affinity for suc (k(0.5) = 0.066 +/- 0.025 mm). atsuc9 showed low substrate specificity, similar to atsuc2 (at1g22710), and transported a wide range of glucosides, including helicin, salicin, arbutin, maltose, fraxin, esculin, turanose, and alpha-me ...	2007	17098854
low cost sorbents for the removal of methyl parathion pesticide from aqueous solutions.	sorptive potential of selected agricultural waste materials i.e. rice (oryza sativa) bran (rb), bagasse fly ash (bfa) of sugarcane (saccharum officinarum), moringa oleifera pods (mop) and rice husk (rh) for the removal of methyl parathion pesticide (mp) from surface and ground waters has been investigated. optimization of operating parameters of sorption process, i.e. sorbent dose, agitation time, ph, initial concentration of sorbate, and temperature have been studied. the sorption data fitted t ...	2007	17109916
immunostimulating effects of the polyphenol-rich fraction of sugar cane (saccharum officinarum l.) extract in chickens.	the phagocytic activity of peripheral blood leukocytes (pbl) in chickens orally administered sugar cane extracts (sce) or polyphenol-rich fraction (prf) of sce (500 mg/kg/day) for 3 consecutive days increased significantly, when compared with that of saline-administered control chickens. chickens orally administered sce or prf (500 mg/kg/day) for 3 consecutive days showed significantly higher antibody responses against sheep red blood cells and brucella abortus than control chickens. in addition ...	2007	17117449
pcdd and pcdf emissions from simulated sugarcane field burning.	the emissions from simulated sugarcane (saccharum officinarum) field burns were sampled and analyzed for polychlorinated dibenzodioxins and dibenzofurans (pcdds and pcdfs). sugarcane leaves from hawaii and florida were burned in a manner simulating the natural physical dimensions and biomass density found during the practice of preharvest field burning. eight composite burn tests consisting of 3-33 kg of biomass were conducted, some with replicate samplers. emission factor calculations using sam ...	2006	17120546
antioxidant activity of phenolics compounds from sugar cane (saccharum officinarum l.) juice.	phenolic compounds in sugar cane (saccharum officinarum l.) juice were identified and quantified by analytical high performance liquid chromatography and photodiode array detection, showing the predominance of flavones (apigenin, luteolin and tricin derivatives), among flavonoids, and of hydroxycinnamic, caffeic and sinapic acids, among phenolic acids, representing a total content of around 160 mg/l. a tricin derivative was present in the highest proportion (>10% of the total). the phenolic extr ...	2006	17123161
morphological aspects of cluster formation in the germarium of the sugarcane borer diatraea saccharalis fabricius (lepidoptera: pyralidae).	diatraea saccharalis f. is one of the greatest pests of the sugar cane culture. this report aimed to characterize the germarium region of the sugarcane borer by light and transmission electron microscopy, emphasizing the morphological steps of the ovarian cluster formation. in the germarium of this insect, four zones could be morphologically identified during the cluster formation. in the most apical end of each ovariole--zone i--the germ line stem cells undergo complete mitotic division, origin ...	2006	17144137
phenanthrene removal by penicillium frequentans grown on a solid-state culture: effect of oxygen concentration.	phenanthrene removal by penicillium frequentans was compared under aerobic and microaerophilic conditions in a solid culture amended with low quantities of an agricultural residue. an inoculum of p. frequentans grown on sugarcane bagasse pith was mixed with soil spiked with 200 mg l(-1) of phenanthrene, to obtain a final bagasse/soil ratio of 1:16. the c/n ratio was adjusted to 60 and the moisture content to 40%. the oxygen concentrations were adjusted to 20%, 10%, 5%, 2% and close to 0%, in the ...	2006	17144256
prospecting the utility of a pmi/mannose selection system for the recovery of transgenic sugarcane (saccharum spp. hybrid) plants.	for the first time, the phosphomannose isomerase (pmi, ec 5.3.1.8)/mannose-based "positive" selection system has been used to obtain genetically engineered sugarcane (saccharum spp. hybrid var. cp72-2086) plants. transgenic lines of sugarcane were obtained following biolistic transformation of embryogenic callus with an untranslatable sugarcane mosaic virus (scmv) strain e coat protein (cp) gene and the escherichia coli pmi gene mana, as the selectable marker gene. postbombardment, transgenic ca ...	2007	17149641
reduced lipopolysaccharide (lps)-induced nitric oxide production in peritoneal macrophages and inhibited lps-induced lethal shock in mice by a sugar cane (saccharum officinarum l.) extract.	a sugar cane extract (sce) has been found to have an immunostimulating effect in several animals. lipopolysaccharide (lps) is known to induce endotoxin shock via the production of inflammatory modulators such as tumor necrosis factor (tnf)-alpha and nitric oxide (no). we examined in the present study the effects of sce on the tnf-alpha and no production in lps-stimulated mice peritoneal cells and the endotoxin shock in mice. the supplementation of sce to peritoneal macrophages cultured with lps ...	2006	17151479
phoenix sylvestris roxb pollen allergy: a 2-year randomized controlled trial and follow-up study of immunotherapy in patients with seasonal allergy in an agricultural area of west bengal, india.	although the efficacy of allergen immunotherapy has been demonstrated in seasonal pollen allergy, there is no report of a double-blind placebo-controlled trial with standardized pollen extract in seasonal respiratory allergy from india. in the agricultural area of eastern india, phoenix sylvestris roxb or date sugar palm is grown or cultivated and seasonal allergic rhinitis is common during the pollen season.	2006	17153886
initial evaluation of sugarcane as a production platform for p-hydroxybenzoic acid.	sugarcane (saccharum hybrids) was evaluated as a production platform for p-hydroxybenzoic acid using two different bacterial proteins (a chloroplast-targeted version of escherichia coli chorismate pyruvate-lyase and 4-hydroxycinnamoyl-coa hydratase/lyase from pseudomonas fluorescens) that both provide a one-enzyme pathway from a naturally occurring plant intermediate. the substrates for these enzymes are chorismate (a shikimate pathway intermediate that is synthesized in plastids) and 4-hydroxyc ...	2005	17168897
response of sugarcane to increasing concentrations of copper and cadmium and expression of metallothionein genes.	sugarcane (saccharum spp.) offers the potential to be a phytoremediator species due to its outstanding biomass production, but its prospective metal accumulation and tolerance have not been fully characterized. sugarcane plantlets were able to tolerate up to 100microm of copper in nutrient solution for 33 days, with no significant reduction in fresh weight, while accumulating 45mgcukg(-1) shoot dry weight. higher levels of copper in solution (250 and 500microm) were lethal. sugarcane displayed t ...	2007	17175063
bioactivity of neem, azadirachta indica, against spittlebug mahanarva fimbriolata (hemiptera: cercopidae) on sugarcane.	the effect of neem, azadirachta indica a. juss, on some biological parameters of mahanarva fimbriolata (stil) (hemiptera: cercopidae) was studied in the laboratory by using neemazal-t/s, nimkol-ls, and an aqueous neem seed extract. initially, the lc,, was estimated for nymphs. later, nymphs fed on sugarcane, saccharum officinarum l., roots were sprayed with the respective lc,, for each product. the adults were maintained in cages on sugarcane plants sprayed at the base with the maximum rate reco ...	2006	17195667
doubled sugar content in sugarcane plants modified to produce a sucrose isomer.	sucrose is the feedstock for more than half of the world's fuel ethanol production and a major human food. it is harvested primarily from sugarcane and beet. despite attempts through conventional and molecular breeding, the stored sugar concentration in elite sugarcane cultivars has not been increased for several decades. recently, genes have been cloned for bacterial isomerase enzymes that convert sucrose into sugars which are not metabolized by plants, but which are digested by humans, with he ...	2007	17207261
production of polyhydroxybutyrate in sugarcane.	we report here the production of the bacterial polyester, polyhydroxybutyrate (phb), in the crop species sugarcane (saccharum spp. hybrids). the phb biosynthesis enzymes of ralstonia eutropha [beta-ketothiolase (phaa), acetoacetyl-reductase (phab) and phb synthase (phac)] were expressed in the cytosol or targeted to mitochondria or plastids. phb accumulated in cytosolic lines at trace amounts, but was not detected in mitochondrial lines. in plastidic lines, phb accumulated in leaves to a maximum ...	2007	17207265
members of the ethylene signalling pathway are regulated in sugarcane during the association with nitrogen-fixing endophytic bacteria.	nitrogen-fixing bacteria have been isolated from sugarcane in an endophytic and beneficial interaction that promotes plant growth. in this work, for the first time, the involvement of ethylene signalling in this interaction was investigated by molecular characterizing members of this pathway in sugarcane. the expression pattern of a putative ethylene receptor (scer1) and two putative erf transcription factors (scerf1 and scerf2) show exclusive modulation in plants inoculated with the diazotrophi ...	2007	17210990
serial analysis of gene expression in sugarcane (saccharum spp.) leaves revealed alternative c4 metabolism and putative antisense transcripts.	sugarcane (saccharum spp.) is a highly efficient biomass and sugar producing crop. leaf reactions have been considered as potential rate-limiting step for sucrose accumulation in sugarcane stalks. to characterize the sugarcane leaf transcriptome, field-grown mature leaves from cultivar "sp80-3280" were analyzed using serial analysis of gene expression (sage). from 480 sequenced clones, 9,482 valid tags were extracted, with 5,227 unique sequences, from which 3,659 (70%) matched at least a sugarca ...	2007	17211512
analysis of sugarcane mosaic virus resistance in maize in an isogenic dihybrid crossing scheme and implications for breeding potyvirus-resistant maize hybrids.	the gene action of 2 sugarcane mosaic virus (scmv) resistance loci in maize, scmv1 and scmv2, was evaluated for potyvirus resistance in an isogenic background. all 4 homozygous and 5 heterozygous isogenic genotypes were produced for introgressions of the resistant donor (fap1360a) alleles at both loci into the susceptible parent (f7) genetic background using simple sequence repeat markers. for scmv and maize dwarf mosaic virus (mdmv), virus symptoms appeared rapidly in the 3 homozygous genotypes ...	2006	17213909
purification and characterization of two sugarcane bagasse-absorbable thermophilic xylanases from the mesophilic cellulomonas flavigena.	we report the purification and characterization of two thermophilic xylanases from the mesophilic bacteria cellulomonas flavigena grown on sugarcane bagasse (scb) as the only carbon source. extracellular xylanase activity produced by c. flavigena was found both free in the culture supernatant and associated with residual scb. to identify some of the molecules responsible for the xylanase activity in the substrate-bound fraction, residual scb was treated with 3 m guanidine hydrochloride and then ...	2007	17219190
mudra-like sequences from rice and sugarcane cluster as two bona fide transposon clades and two domesticated transposases.	the mutator system of maize has been described as the most active and mutagenic plant transposon. the autonomous element mudr contains two genes: mudra encoding the transposase and mudrb whose product function remains undetermined. mudra-like coding domain showed to be the most abundantly expressed transposon-related sequence in sugarcane transcriptome. a previous report identified the existence of at least four clades of mudra-like sequences in sugarcane, rice and arabidopsis, which already exi ...	2007	17289300
effects of a mixture of fatty acids from sugar cane (saccharum officinarum l.) wax oil in two models of inflammation: zymosan-induced arthritis and mice tail test of psoriasis.	a mixture of fatty acids obtained from sugar cane (saccharum officinarum l.) wax oil (fam), in which the main constituents are palmitic, oleic, linoleic, and linolenic acids, was evaluated in two models of inflammation: zymosan-induced arthritis and in the tail test for psoriasis, both on mice. in the first model, fam significantly reduced zymozan-induced increase of beta glucuronidase (de(50) 90+/-7 mg/kg). histopathological studies showed inhibition in cellular infiltration and reduction of sy ...	2007	17292594
comparison of composition and absorption of sugarcane policosanols.	policosanols (pc) exist as very-long-chain alcohols derived from sugarcane currently used in many countries as a cholesterol-lowering therapy. pc purity and relative percentage composition have been suggested as primary reasons why the original cuban pc (opc) supplements possess lipid-lowering efficacy. the purpose of the present study was, first, to compare the relative percentage purity and pc composition of both opc and alternative sources of pc (apc). a second objective was to feed syrian ha ...	2007	17298709
growth and metabolism in sugarcane are altered by the creation of a new hexose-phosphate sink.	an efficient in planta sugarcane-based production system may be realized by coupling the synthesis of alternative products to the metabolic intermediates of sucrose metabolism, thus taking advantage of the sucrose-producing capability of the plant. this was evaluated by synthesizing sorbitol in sugarcane (saccharum hybrids) using the malus domestica sorbitol-6-phosphate dehydrogenase gene (mds6pdh). mature transgenic sugarcane plants were compared with untransformed sugarcane variety q117 by eva ...	2007	17309679
heterologous signals allow efficient targeting of a nuclear-encoded fusion protein to plastids and endoplasmic reticulum in diverse plant species.	approximately 30% of plant nuclear genes appear to encode proteins targeted to the plastids or endoplasmic reticulum (er). the signals that direct proteins into these compartments are diverse in sequence, but, on the basis of a limited number of tests in heterologous systems, they appear to be functionally conserved across species. to further test the generality of this conclusion, we tested the ability of two plastid transit peptides and an er signal peptide to target green fluorescent protein ...	2007	17309684
agrobacterium-mediated transformation of cereals: a promising approach crossing barriers.	cereal crops have been the primary targets for improvement by genetic transformation because of their worldwide importance for human consumption. for a long time, many of these important cereals were difficult to genetically engineer, mainly as a result of their inherent limitations associated with the resistance to agrobacterium infection and their recalcitrance to in vitro regeneration. the delivery of foreign genes to rice plants via agrobacterium tumefaciens has now become a routine techniqu ...	2006	17309731
biochemical and biophysical characterization of small heat shock proteins from sugarcane. involvement of a specific region located at the n-terminus with substrate specificity.	when cells are submitted to an increase in temperature, heat shock proteins (hsp) are synthesized to help heat stress resistance. small hsps, which are diverse and abundant in plants, have the major function of preventing irreversible protein aggregation. the diversity of small hsps in plants is intriguing and characterization of their chaperone activity is important to understand plant tolerance to heat stress. a previous study showed that small hsps, mainly represented by class i (cytosolic), ...	2007	17336576
domestication of maize, sorghum, and sugarcane did not drive the divergence of their smut pathogens.	we investigated two alternative hypotheses for the origin of crop pathogen species: that human-mediated agricultural practices drove the divergence of many crop plant pathogen species or that coevolutionary processes in natural populations of the crops' ancestors drove divergence of pathogen species. we distinguished between these two hypotheses by constructing a robust multigene phylogeny and estimating the dates of divergence among four, monophyletic species of smut fungi (ustilago maydis, u. ...	2007	17348948
antiproliferative and antioxidant activities of a tricin acylated glycoside from sugarcane (saccharum officinarum) juice.	from sugarcane juice, a flavone, identified by spectroscopic methods as tricin-7-o-beta-(6''-methoxycinnamic)-glucoside, was isolated, in addition to orientin. the tricin derivative was shown to have antioxidant activity higher than trolox by means of the dpph assay and lower by the beta-carotene/linoleic acid system. it showed in vitro antiproliferative activity against several human cancer cell lines, with higher selectivity toward cells of the breast resistant nic/adr line.	2007	17350657
signal transduction-related responses to phytohormones and environmental challenges in sugarcane.	sugarcane is an increasingly economically and environmentally important c4 grass, used for the production of sugar and bioethanol, a low-carbon emission fuel. sugarcane originated from crosses of saccharum species and is noted for its unique capacity to accumulate high amounts of sucrose in its stems. environmental stresses limit enormously sugarcane productivity worldwide. to investigate transcriptome changes in response to environmental inputs that alter yield we used cdna microarrays to profi ...	2007	17355627
sugarcane borer (lepidoptera: crambidae) resistance to transgenic bacillus thuringiensis maize.	transgenic maize, zea mays l., expressing the bacillus thuringiensis (bt) crylab toxin has been planted to extensive areas across the united states and several other countries, but no resistance has been documented in field populations oflepidopteran target pests. this article describes the first report of resistance alleles to commercially available cry1ab bt maize in a louisiana population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). two hundred thirteen two-parent i ...	2007	17370824
how safe is sugarcane juice?		2007	17377362
enumeration, isolation and identification of diazotrophs from korean wetland rice varieties grown with long-term application of n and compost and their short-term inoculation effect on rice plants.	this study has been aimed (i) to isolate and identify diazotrophs from korean rice varieties; (ii) to examine the long-term effect of n and compost on the population dynamics of diazotrophs and (iii) to realize the shot-term inoculation effect of these diazotrophs on rice seedlings.	2007	17381741
pathogenicity of fusarium semitectum against crop pests and its biosafety to non-target organisms.	microbial control is receiving more attention, since these alternative tactics, compared to chemical control methods, are energy saving, non polluting, ecologically sound and sustainable. a mycopathogen, fusarium semitectum berk. and rav. (arsef 7233) was isolated from diseased cadavers of aphid (aphis gossypii) and cultured in saboraud maltose agar supplemented with yeast extract medium (smay). being isolated first time from the chilli ecosystem its potential was evaluated. experiments were con ...	2006	17385514
enhanced biodegradation of hexachlorocyclohexane (hch) in contaminated soils via inoculation with sphingobium indicum b90a.	soil pollution with hexachlorocyclohexane (hch) has caused serious environmental problems. here we describe the targeted degradation of all hch isomers by applying the aerobic bacterium sphingobium indicum b90a. in particular, we examined possibilities for large-scale cultivation of strain b90a, tested immobilization, storage and inoculation procedures, and determined the survival and hch-degradation activity of inoculated cells in soil. optimal growth of strain b90a was achieved in glucose-cont ...	2008	17387620
mortality staging of dominant lethals induced in the f1 generation of the sugarcane borer; diatraea saccharalis (f.).	comparative egg maturation was observed between fertile eggs laid by unirradiated females, by unirradiated females mated with irradiated males, by irradiated females mated with normal males, and by virgin unirradiated females. haploid eggs turn yellow and dry by the fifth day. eggs from irradiated parents develop more slowly than normal eggs. most embryos from parents irradiated as p generation adults at 16.8 kilorads for males and 12.0 kr for females die early in embryonic development. a descri ...	1968	17387934
identification of prevalent potyvirus on maize and johnsongrass in corn fields of tehran province of iran and a study on some of its properties.	during a growing season in 2004, 231 leaf samples of virus infected and mosaic and dwarf mosaic symptoms showing maize (zea mays l.) plants and 258 leaf samples of mosaic showing johnsongrass (sorghum halepens l.) plants from various corn fields in tehran province were collected. serological tests of das-elisa and diba were performed on samples using antisera of sugarcane mosaic virus (scmv), maize dwarf mosaic virus (mdmv), sorghum mosaic virus (srmv) and johnsongrasss mosaic virus (jgmv). in b ...	2006	17390895
biotransformation of cannabidiol to cannabielsoin by suspension cultures of cannabis sativa and saccharum officinarum.	suspension cultures of cannabis sativa l. and saccharum officinarum l. have been shown to biotransform cannabidiol to cannabielsoin under normal growth conditions. both cannabielsoin c-1 stereoisomers were produced. mass spectral and chromatographic evidence were used in this identification. this is the first reported biologically catalyzed synthesis of cannabielsoin as well as the first biotransformation of any cannabinoid by plant tissue culture systems. possible mechanisms and significance of ...	1983	17404934
water stress augments silicon-mediated resistance of susceptible sugarcane cultivars to the stalk borer eldana saccharina (lepidoptera: pyralidae).	silicon (si) can improve resistance of plants to insect attack and may also enhance tolerance of water stress. this study tested if si-mediated host plant resistance to insect attack was augmented by water stress. four sugarcane cultivars, two resistant (n21, n33) and two susceptible (n26, n11) to eldana saccharina walker were grown in a pot trial in si-deficient river sand, with (si+) and without (si-) calcium silicate. to induce water stress, irrigation to half the trial was reduced after 8.5 ...	2007	17411480
orthologous comparison in a gene-rich region among grasses reveals stability in the sugarcane polyploid genome.	modern sugarcane (saccharum spp.) is an important grass that contributes 60% of the raw sugar produced worldwide and has a high biofuel production potential. it was created about a century ago through hybridization of two highly polyploid species, namely s. officinarum and s. spontaneum. we investigated genome dynamics in this highly polyploid context by analyzing two homoeologous sequences (97 and 126 kb) in a region that has already been studied in several cereals. our findings indicate that t ...	2007	17425713
biotechnological production of xylitol in a three-phase fluidized bed bioreactor with immobilized yeast cells in ca-alginate beads.	cells of candida guilliermondii entrapped in ca-alginate beads were used for xylitol production, from concentrated hemicellulose hydrolyzate of sugarcane bagasse, in a fluidized bed bioreactor (fbr). the maximum xylitol concentration 28.9 g xylitol/l was obtained at a high aeration rate of 600 ml/min after 70 h of fermentation, indicating that the use of high aeration rate in this system is favored for better oxygen transfer into the immobilized cells. the specific xylitol productivity and the x ...	2007	17427994
pipeline for macro- and microarray analyses.	the pipeline for macro- and microarray analyses (pmma) is a set of scripts with a web interface developed to analyze dna array data generated by array image quantification software. pmma is designed for use with single- or double-color array data and to work as a pipeline in five classes (data format, normalization, data analysis, clustering, and array maps). it can also be used as a plugin in the bioarray software environment, an open-source database for array analysis, or used in a local versi ...	2007	17464422
influence of inhibitory compounds and minor sugars on xylitol production by debaryomyces hansenii.	to obtain in-depth information on the overall metabolic behavior of the new good xylitol producer debaryomyces hansenii ufv-170, batch bioconversions were carried out using semisynthetic media with compositions simulating those of typical acidic hemicellulose hydrolysates of sugarcane bagasse. for this purpose, we used media containing glucose (4.3-6.5 g/l), xylose (60.1-92.1 g/l), or arabinose (5.9-9.2 g/l), or binary or ternary mixtures of them in either the presence or absence of typical inhi ...	2007	17496338
potential of plant extracts in combination with bacterial antagonist treatment as biocontrol agent of red rot of sugarcane.	plant extracts and antifungal microorganisms were tested singly and in combination for biocontrol of sugarcane red rot disease (colletotrichum falcatum) using two sugarcane (saccharum officinarum l.) cultivars, coc671 and coc92061, in pot and field experiments. leaf extracts of abrus precatorius and bassia latifolia and the rhizome extract of curcuma longa reduced colletotrichum falcatum mycelial growth by 80%, 58%, and 57%, respectively. although sugarcane- planting materials (setts) treated in ...	2007	17496967
bioformulation of burkholderia sp. mssp with a multispecies consortium for growth promotion of cajanus cajan.	the present work was undertaken to formulate an effective bioformulation using burkholderia sp. strain mssp, a known plant-growth-promoting rhizobacterium. mssp was tagged with the reporter gene of green fluorescent protein (gfp) to monitor its population in cost-effective solid carriers, including sugarcane-bagasse, sawdust, cocoa peat, rice husk, wheat bran, charcoal, and rock phosphate, and paneer-whey as liquid carrier. physical and chemical properties of different low-cost carrier materials ...	2007	17496969
resource recovery potential from secondary components of segregated municipal solid wastes.	fermentable components of municipal solid wastes (msw) such as fruit and vegetable wastes (fvw), leaf litter, paddy straw, cane bagasse, cane trash and paper are generated in large quantities at various pockets of the city. these form potential feedstocks for decentralized biogas plants to be operated in the vicinity. we characterized the fermentation potential of six of the above msw fractions for their suitability to be converted to biogas and anaerobic compost using the solid-state stratified ...	2007	17503209
sugarcane biopolymer patch in femoral artery angioplasty on dogs.	the objective of this study was to evaluate the use of the sugarcane biopolymer membrane in femoral artery patch angioplasty on dogs.	2007	17505660
biotechnological production of gluconic acid: future implications.	gluconic acid (ga) is a multifunctional carbonic acid regarded as a bulk chemical in the food, feed, beverage, textile, pharmaceutical, and construction industries. the favored production process is submerged fermentation by aspergillus niger utilizing glucose as a major carbohydrate source, which accompanied product yield of 98%. however, use of ga and its derivatives is currently restricted because of high prices: about us$ 1.20-8.50/kg. advancements in biotechnology such as screening of micro ...	2007	17525864
complete chloroplast genome sequences of hordeum vulgare, sorghum bicolor and agrostis stolonifera, and comparative analyses with other grass genomes.	comparisons of complete chloroplast genome sequences of hordeum vulgare, sorghum bicolor and agrostis stolonifera to six published grass chloroplast genomes reveal that gene content and order are similar but two microstructural changes have occurred. first, the expansion of the ir at the ssc/ira boundary that duplicates a portion of the 5' end of ndhh is restricted to the three genera of the subfamily pooideae (agrostis, hordeum and triticum). second, a 6 bp deletion in ndhk is shared by agrosti ...	2007	17534593
production of d-lactic acid from sugarcane molasses, sugarcane juice and sugar beet juice by lactobacillus delbrueckii.	lactobacillus delbrueckii was grown on sugarcane molasses, sugarcane juice and sugar beet juice in batch fermentation at ph 6 and at 40 degrees c. after 72 h, the lactic acid from 13% (w/v) sugarcane molasses (119 g total sugar l(-1)) and sugarcane juice (133 g total sugar l(-1)) was 107 g l(-1) and 120 g l(-1), respectively. with 10% (w/v) sugar beet juice (105 g total sugar l(-1)), 84 g lactic acid l(-1) was produced. the optical purities of d: -lactic acid from the feedstocks ranged from 97.2 ...	2007	17541505
kinetic model of sucrose accumulation in maturing sugarcane culm tissue.	biochemically, it is not completely understood why or how commercial varieties of sugarcane (saccharum officinarum) are able to accumulate sucrose in high concentrations. such concentrations are obtained despite the presence of sucrose synthesis/breakdown cycles (futile cycling) in the culm of the storage parenchyma. given the complexity of the process, kinetic modelling may help to elucidate the factors governing sucrose accumulation or direct the design of experimental optimisation strategies. ...	2007	17555779
ecological occurrence of gluconacetobacter diazotrophicus and nitrogen-fixing acetobacteraceae members: their possible role in plant growth promotion.	gluconacetobacter diazotrophicus has a long-standing history of bacterial-plant interrelationship as a symbiotic endophyte capable of fixing atmospheric nitrogen. in low nitrogen fertilized sugarcane fields it plays a significant role and its occurrence was realised in most of the sugarcane growing countries. in this mini review, the association of g. diazotrophicus with sugarcane, other crop plants and with various hosts is discussed. the factors affecting survival in the rhizosphere and the pu ...	2008	17574542
molecular cloning and biochemical characterization of a family-9 endoglucanase with an unusual structure from the gliding bacteria cytophaga hutchinsonii.	cytophaga hutchinsonii was originally isolated from sugarcane piles. this microorganism therefore probably produces an array of enzymes allowing it to digest cellulosic substrates. c. hutchinsonii thus represents a rich source of potentially effective cellulase enzymes that can be harnessed for conversion of biomass to simple sugars. these sugars can then be used as feedstock for ethanol production or other chemical syntheses. in this study, we report the pcr cloning of an endoglucanase gene (ce ...	2007	17625271
substrate specificity-conferring regions of the nonribosomal peptide synthetase adenylation domains involved in albicidin pathotoxin biosynthesis are highly conserved within the species xanthomonas albilineans.	albicidin is a pathotoxin produced by xanthomonas albilineans, a xylem-invading pathogen that causes leaf scald disease of sugarcane. albicidin is synthesized by a nonribosomal pathway via modular polyketide synthase and nonribosomal peptide synthetase (nrps) megasynthases, and nrps adenylation (a) domains are responsible for the recognition and activation of specific amino acid substrates. dna fragments (0.5 kb) encoding the regions responsible for the substrate specificities of six albicidin n ...	2007	17630307
alpha-amylase production from catabolite derepressed bacillus subtilis kcc103 utilizing sugarcane bagasse hydrolysate.	a catabolite derepressed bacillus subtilis strain kcc103 was used to produce alpha-amylase in medium containing sugarcane bagasse hydrolysate (sbh). addition of sbh (1% reducing sugar (w/v)) to the nutrient medium supported maximum alpha-amylase production of 67.4 um l(-1). hplc analysis of sbh showed the presence of glucose, xylose and arabinose in the ratio of 0.9:1.0:0.16 (w/w/w). in sbh-medium glucose and xylose were consumed completely while arabinose remained unutilized. uptake rate of glu ...	2008	17644331
xanthan production by xanthomonas albilineans infecting sugarcane stalks.	xanthomonas albilineans is the causal organism of leaf scald, a bacterial vascular disease of sugarcane. xanthomonas may invade the parenchyma between the bundles and cause reddened pockets of gum, identified as a xanthan-like polysaccharide. since xanthan contains glucuronic acid, the ability of xanthomonas to produce an active udp glucose dehydrogenase is often seen as a virulence factor. x. albilineans axenically cultured did not secrete xanthans to willbrink liquid media, but the use of inoc ...	2008	17646030
synthesis of [3-(3-chloro-4-fluorophenyl)-2-oxo-3,4-dihydro-2h-2lambda5- benzo[e][1,3,2]oxazaphosphinin-2-yl]-(aryl/alkyl) methanols and their bioactivity on sugarcane smut.	synthesis of some new substituted [3-(3-chloro-4-fluorophenyl)-2-oxo-3,4-dihydro-2h-2lambda(5)-benzo[e][1,3,2]oxazaphosphinin-2-yl]-(aryl/alkyl)methanols (7a-k) based on the pudovick reaction was accomplished in the presence of niobium pentoxide (nb(2)o(5)) without using an external chiral ligand. nb(2)o(5) appears to form the metal complex intermediate catalyst system (6) by reacting with 3-(3-chloro-4-fluoro-phenyl)-3,4-dihydrobenzo[e][1,3,2]oxazaphosphinine-2-oxide (4), which not only directs ...	2007	17661484
complete genome sequences of maize dwarf mosaic and sugarcane mosaic virus isolates coinfecting maize in spain.	the genomes of spanish isolates of maize dwarf mosaic virus (mdmv-sp) and sugarcane mosaic virus (scmv-sp) were completely sequenced. nucleotide sequence identities of scmv-sp to those of other scmv isolates ranged from 79 to 90%. mdmv-sp shared 85% nucleotide identity with the only other fully sequenced isolate of mdmv. mdmv-sp and scmv-sp differed from each other by 31% in their nucleotide sequences. phylogenetic analyses showed that scmv isolates group by host rather than by geographical loca ...	2007	17680319
a characterization of a mutm/fpg ortholog in sugarcane--a monocot plant.	plant genomic projects, such as arabidopsis thaliana, rice, and maize, have provided excellent tools for comparative genome analysis on base excision dna repair (ber). a data-mining study associated with the sucest genome project identified two est clusters that shared homology to the bacteria mutm/fpg protein. comparative analyses presented here show a duplication of the mutm/fpg gene in sugarcane, wheat and rice. the complementation assays show that both cdnas from sugarcane are able to comple ...	2007	17686457
heterologous expression of new antifungal chitinase from wheat.	chitinases (ec 3.2.1.14) have been grouped into seven classes (class i-vii) on the basis of their structural properties. chitinases expressed during plant-microbe interaction are involved in defense responses of host plant against pathogens. in the present investigation, chitinase gene from wheat has been subcloned and overexpressed in escherichia coli bl-21 (de3). molecular phylogeny analyses of wheat chitinase indicated that it belongs to an acidic form of class vii chitinase (glycosyl hydrola ...	2007	17697785
identification of genes responsive to the application of ethanol on sugarcane leaves.	the control of gene expression in precise time and space is a desirable attribute of chemically inducible systems. ethanol is a chemical inducer with favourable features, such as being inexpensive and easy to apply. the aim of this study was to identify ethanol-responsive genes in sugarcane. the cdna macroarray technique was adopted to identify transcript changes in sugarcane leaves (saccharum spp. cv sp80-3280) exposed to ethanol. the expression profiles of sugarcane genes were analysed using n ...	2007	17701412