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the complete amino acid sequence of trypsin inhibitor de-3 from erythrina latissima seeds.trypsin inhibitor de-3 from erythrina latissima seeds contains 172 amino acids, including 4 half-cystine residues, and resembles the kunitz-type inhibitors. limited hydrolysis of de-3 with trypsin at ph 3 produced two fragments, f1 and f2, containing 63 and 109 amino acids, respectively. amino-terminal sequence studies revealed that f1 was the n-terminal and that f2 was the c-terminal fragment. the complete amino acid sequence of fragments f1 and f2 was then determined on peptides produced by en ...19853902809
an immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat.ten different fluorescein-conjugated lectins of various known sugar specificities were used to study cell surface glycoconjugates of normal and regenerating rat skeletal muscle. in normal muscle, canavalia ensiformis agglutinin, triticum vulgaris agglutinin (wheat germ agglutinin, wga), ricinus communis agglutinin-i, and maclura pomifera agglutinin bound strongly to the endomysial region of the myofibers. no binding was observed in the cytoplasm of the myofibers. other lectins (dolichos biflorus ...19853915936
lectin characterization of gonococci from an outbreak caused by penicillin-resistant neisseria gonorrhoeae.a total of 40 neisseria gonorrhoeae isolates, representing 19 penicillin-resistant isolates (from 8 heterosexual patients and 11 homosexual patients) and 21 penicillin-susceptible isolates (from 15 heterosexual patients and 6 homosexual patients) and obtained from the same geographic area, were examined. lectin agglutination patterns were based on the reactivity of the isolates with the following 14 lectins: concanavalin a, lens culinaris, trichosanthes kinlowii, griffonia simplicifolia i, arach ...19853935658
aflatoxin-induced alterations in glycine max, cv. 'essex' root cell membrane protein content.aflatoxins (afts) are hepatocarcinogens, mutagens, teratogens and toxins. isolates of afts-producing strains of aspergillus flavus can grow upon both autoclaved soybeans and to a lesser extent field-grown beans. besides inhibiting the elongation of excised, in vitro cultured soybean roots, the afts can impair the roots' ability to remove [14c]-leucine from a culture medium and to incorporate the amino acid into acid-insoluble, cytoplasmic protein. in this connection, exogenous afts once taken-up ...19853935929
enzymatic properties of the lipoxygenase from pea seeds.a lipoxygenase preparation was obtained from dried green pea seeds. disc electrophoresis with enzyme staining indicated the presence of only one main isoenzyme corresponding to the isoenzyme pl i according to yoon and klein (j. agric. food chem. 24, 955 (1979)), whereas pl ii was absent. the assay for pea lipoxygenase has been optimized by using a final concentration of 0.53 mm potassium linoleate in the presence or absence of 0.2% sodium cholate. without detergent the rho h optimum was 5.9, in ...19853936491
genes for low-molecular-weight heat shock proteins of soybeans: sequence analysis of a multigene family.soybeans, glycine max, synthesize a family of low-molecular-weight heat shock (hs) proteins in response to hs. the dna sequences of two genes encoding 17.5- and 17.6-kilodalton hs proteins were determined. nuclease s1 mapping of the corresponding mrna indicated multiple start termini at the 5' end and multiple stop termini at the 3' end. these two genes were compared with two other soybean hs genes of similar size. a comparison among the 5' flanking regions encompassing the presumptive hs promot ...19853939317
effects of cone opening, initial moisture content and multiple extrusion on the protein quality of extruded soybean using the brady crop cooker.in the present study, three cone openings (0.133; 0.106, and 0.080 cm) and three initial moisture content values (9%, 15% and 21%) were used as treatments to evaluate their effects on the protein quality of full-fat soybean flour, extruded in the brady crop cooker. the specific volume, protein and oil contents as well as available lysine content characteristic of the final product, were not affected by the treatments used. processing temperatures, however, decreased when the initial moisture con ...19853939487
interactions between lectins and other components of leguminous protein bodies.previous work from this laboratory had shown that leguminosa seed extracts contain lectin-bound proteins. in the present paper, the isolation of protein bodies from the seeds of 7 leguminosa species (canavalia ensiformis, lens culinaris, pisum sativum, glycine max, sophora japonica, wisteria floribunda and phaseolus vulgaris) is described. protein bodies were characterized microscopically and by their constituents, storage proteins, lectins and some glycosidases. from the protein bodies, lectin- ...19863954886
exopolysaccharides of the phytopathogen pseudomonas syringae pv. glycinea.exopolysaccharides (eps) of the soybean pathogen pseudomonas syringae pv. glycinea were isolated from culture filtrates and infected soybean leaves. levan (a polyfructan with a c-2----c-6 backbone and c-2----c-1 branching) or acetylated alginate (a linear polyuronide of c-1----c-4-linked mannuronic and guluronic acids) was isolated from culture filtrates when bacterial strains were grown in a semisynthetic medium containing sucrose or glucose, respectively, as the primary carbon source. acetylat ...19863957873
identification of rhizobium plasmid sequences involved in recognition of psophocarpus, vigna, and other legumes.symbiotic dna sequences involved in nodulation by rhizobium must include genes responsible for recognizing homologous hosts. we sought these genes by mobilizing the symbiotic plasmid of a broad host-range rhizobium mpik3030 (= ngr234) that can nodulate glycine max, psophocarpus tetragonolobus, vigna unguiculata, etc., into two nod- rhizobium mutants as well as into agrobacterium tumefaciens. subsequently, cosmid clones of pmpik3030a were mobilized into nod+ rhizobium that cannot nodulate the cho ...19863958042
race:cultivar-specific induction of enzymes related to phytoalexin biosynthesis in soybean roots following infection with phytophthora megasperma f. sp. glycinea.primary roots of soybean [glycine max (l.), cv harosoy 63] seedlings were inoculated with zoospores from either race 1 (incompatible, host resistant) or race 3 (compatible, host susceptible) of phytophthora megasperma f. sp. glycinea (pmg) and the activities of phenylalanine ammonia-lyase (pal), chalcone synthase (chs), isoflavone synthase, and dihydroxypterocarpan 6a-hydroxylase related to phytoalexin (glyceollin) biosynthesis, and of glucose-6-phosphate dehydrogenase (glc-6-pdh) and glutamate ...19863963819
partial purification and characterization of two forms of malonyl-coenzyme a:acyl carrier protein transacylase from soybean leaf tissue.investigation of malonyl-coa:acyl carrier protein transacylase from soybeans has shown that this fatty acid biosynthetic enzyme occurs in at least two isozymic forms. both forms exist as soluble, low-molecular-mass polypeptides (approx 43 kda) which catalyze one of the first committed steps in the synthesis of c16 and c18 fatty acids. we have partially purified the two forms of this enzyme from soybean leaf tissue 1200- and 3900-fold respectively. isozyme 1 does not adhere to ion-exchange or blu ...19863963823
isolation and characterization of a soybean lectin having 4-o-methylglucuronic acid specificity.a new lectin from soybeans having specificity toward the extracellular 4-o-methyl-d-glucurono-l-rhamnans produced by certain strains of rhizobium japonicum has been purified and characterized. isolation was accomplished initially by isoelectric precipitation of contaminating globulins and subsequently by affinity chromatography on partially hydrolyzed glucuronorhamnan covalently coupled to amino-hexylagarose. residual globulins were removed by adsorption of the lectin on concanavalin a-agarose a ...19863964668
legume agglutinins that bind to rhizobium meliloti.a protein found in seeds and roots of alfalfa (medicago sativa) was implicated in the specificity of the infection process, based on its binding to the symbiont rhizobium meliloti. we found an agglutinin with similar properties in seeds and roots of sweet clover (melilotis alba). the sweet clover differed from alfalfa in nodulation by a mutant strain of r. meliloti, but the agglutinins were indistinguishable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, rhizobium agglutination, a ...19853988714
assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism.the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ...19854016573
liquid chromatographic determination of protein acid hydrolysate content in blended soy sauce.five methods were investigated for the determination of levulinic acid in soy sauce to determine the addition of protein hydrolysate, mainly acid hydrolysate of defatted soybeans. best results were obtained by using liquid chromatography (lc) with 0.004 m hclo4 as the mobile phase and bromcresol purple as a post-column reagent. an innovative lc method with 0.1% h3po4 as eluant was developed for determination of levulinic acid at 280 nm in soy sauce. this was the most time-saving method.20064030629
raw soybeans for gestating swine.an experiment was conducted to determine the influence of feeding ground raw soybeans to swine during gestation on reproductive performance for three consecutive parities. sixty crossbred gilts were bred and randomly assigned to two dietary treatments with 30 gilts/treatment. the diets were formulated to contain 14% crude protein with either soybean meal (sbm) or raw soybeans (rsb) serving as the source of supplemental protein. all animals were fed in individual gestation stalls and were given 1 ...19854038700
physiological and pathobiological significance of ocular glycoproteins. i. studies using fluorescein labelled glycine max.cell surface carbohydrates play an important role in several biological, immunological, and neoplastic phenomena including development, growth regulation, cellular locomotion, receptor activation, and tumour metastasis. fluorescein labelled lectins which bind to specific carbohydrate residues in glycoproteins and glycolipids are being increasingly used as chemical probes to study cell components. several different preparations of ocular tissues from human, rabbit, and rat were examined for the d ...19854038883
protein degradation in rumen and amino acid absorption in small intestine of lactating dairy cattle fed heat-treated whole soybeans.four lactating holstein cows fitted with rumen cannulae and t-type cannulae in proximal duodenum and terminal ileum were used to measure protein degradation in the rumen and amino acid flow and absorption in the small intestine. soybean meal, whole soybeans, and whole soybeans extruded at 132 and 149 degrees c provided 50% of the protein in diets that contained 51% grain, 36% corn silage, and 13% alfalfa hay (dry matter). spot samples of digesta were collected from duodenum and ileum during 96 h ...19854038985
[ph3 residues in hazelnuts, soybeans and wheat following phosphine fumigation with non-constant concentrations].in model tests hazelnuts, soy beans and wheat were fumigated with phosphine (ph3) at non constant concentrations. the influence of different concentration characteristics on the fumigation and the decomposition of phosphine residues was investigated in accordance with the fumigation technique. at the beginning the concentration increases, and after attaining the maximum gradually decreases to zero. the level of residues during the fumigation as well as the behaviour of residues during the storag ...20144039093
coordinate expression of ribosomal protein mrnas following auxin treatment of soybean hypocotyls.nine recombinant plasmids containing soybean (glycine max. var. wayne) ribosomal protein mrna sequences were identified by hybrid selection-translation followed by gel electrophoresis. individual plasmids were used to determine the amount of ribosomal protein mrna in soybean hypocotyls at various times after application of the synthetic auxin (2,4-dichlorophenoxy)acetic acid. results from these experiments indicate that the relative abundance of ribosomal protein mrnas increases 3-8-fold within ...19854039725
protection against dimethylhydrazine-induced adenomatous tumors of the mouse colon by the dietary addition of an extract of soybeans containing the bowman-birk protease inhibitor.dimethylhydrazine (dmh) induces adenocarcinomas of the colon and rectum in rodents; this animal cancer model system is thought to closely resemble human colorectal cancer. an extract of soybeans containing the bowman-birk protease inhibitor added to the diet of dmh-treated mice resulted in a significant suppression of adenomatous tumors of the colonic mucosa. non-treated mice maintained on the same diet for most of their natural life-span (1.5 years) showed no observable health problems.19854040444
[urticaria due to the soybeans]. 19854040584
preparation of photoreactive derivatives of trypsin-chymotrypsin inhibitors from soybeans and chick peas by selective modification of lysine residues.photoreactive derivatives of the bowman-birk trypsin-chymotrypsin inhibitor (bbi) from soybeans and of ci, the trypsin-chymotrypsin inhibitor from chick peas, were prepared by selective modification of the epsilon-amino groups of lysine residues with 2-nitro-4(5)-azidophenylsulfenyl chlorides (2,4(5)-naps-c1). the ultraviolet absorption spectra of the photolabeled inhibitors indicated that three out of the five lysines of bbi and one of the seven lysines of ci were modified. the inhibitory activ ...20144055235
effect of phytate on aflatoxin formation by aspergillus parasiticus grown on different grains.aflatoxin production by aspergillus parasiticus on corn, soybean, and cottonseed in the absence or presence of added sodium phytate was examined. no variation in aflatoxin concentrations was found in raw, chemically sterilized, or autoclaved soybeans whereas a five-fold reduction in total aflatoxins was found in cottonseed after addition of 330 micrograms sodium phytate to 10 g of autoclaved material. however, phytate did not affect aflatoxin production on non-sterile cottonseeds, although in co ...19854069189
discrepancies among published amino acid sequences of soybean leghemoglobins: experimental evidence against cultivar differences as the sources of the discrepancies.despite discrepancies among charged amino acid residues in published amino acid sequences, isoelectric focusing experiments failed to detect varietal differences in soybean leghemoglobins a, c1, c2, or c3. leghemoglobins from 69 domesticated soybean (glycine max) cultivars and plant introductions and 18 wild soybean (glycine soja) plant introductions were compared; the sources included soybean cultivars used by research groups in obtaining amino acid sequences and most of the ancestors of north ...19854083896
isocitrate dehydrogenase of soybeans. 19744154916
aflatoxin production in some varieties of soybeans (glycine max l.). 19734202363
the effect on duckling of a diet containing moulded-soybeans. 20144231974
studies on the glycolipids and phospholipids of immature soybeans. 19704318123
[a method for preparing radioactive labeled phosphatides using soybeans in vivo]. 19714398281
[characterization of a trypsin-like proteinase (acrosin) from boar spermatozoa by inhibition with different protein proteinase inhibitors, ii. inhibitors from leeches, soybeans, peanuts, bovine colostrum and sea anemones]. 19724403328
studies in mice on the isolation and characterization of growth inhibitors from soybeans. 19744472324
effect of some pelleting agents and sodium humate as spray on dry matter yield and nutrient uptake of soybean (glycine max l. cv bragg) in a saline-sodic soil. 19744480492
inclusion of a macaroni composed of opaque-2 corn, soybeans and wheat in the food of children aged from 1 to 4 years. 19734496225
unidentified factor activities in whole soybeans required for optimum growth of coturnix quail. 19724563004
biological and physiological factors in soybeans. 19744592393
a review of flavor investigations associated with the soy products raw soybeans, defatted flakes and flours, and isolates. 20084593855
powdery mildew of soybeans. 20004612359
occurrence of aflatoxins and aflatoxin-producing strains of aspergillus spp. in soybeans.above average rainfall in maryland during august, september, and october 1971 resulted in heavy mold growth in soybeans while still in the field. of 28 samples of soybean seed, aflatoxins were found in 14, 2 of which had been used in poultry feed. aflatoxins were identified by thin-layer chromatography, spectrophotometry, and chicken embryo bioassay. aspergillus spp. were isolated from 11 samples, and 5 of these isolates produced aflatoxins when grown in liquid culture.19724673021
release of bound trypsin inhibitors in soybeans by rhizopus oligosporus. 19724673242
synthesis of p-coumaroyl coenzyme a with a partially purified p-coumarate:coa ligase from cell suspension cultures of soybean (glycine max). 19734699252
heat inactivation of trypsin inhibitor, lipoxygenase and urease in soybeans: effect of acid and base additives. 19734736350
phosphine residues on soybeans fumigated with aluminum phosphide. 19734736354
phorate and aldicarb: effects of systemic fungicides on their levels in soybeans. 19734736355
isolation and characterization of bacteriophage sbx-1 and its bacterial host, both endemic in soybeans.a phage, sbx-1, and its bacterial host, xanthomonas sp. 1, were isolated consistently from roots, internal portions of stems, and leaves of soybean plants. phage titer in leaves was highly variable. it was very low in seedlings, reached a maximum of 10(4) pfu/ml of sap after 11 weeks of plant growth and again dropped to very low levels. we isolated sbx-1 from plants of all 45 varieties studied, but not consistently from some. plants of some varieties also carried xanthomonas sp. 2, which was res ...19734737642
contribution of trypsin inhibitors to the deleterious effects of unheated soybeans fed to rats. 19734796250
comparisons of nitrogen fixation estimates in soybeans by nodule weight, leghemoglobin content, and acetylene reduction. 19734796348
studies on the lipid composition of developing soybeans. 19734796874
somatic crossing over in glycine max (l.) merrill: mutagenicity of sodium azide and lack of synergistic effect with caffeine and mitomycin c.glycine max (soybean) is one angiosperm which lends itself to the study of somatic crossing over. this is made possible because some varieties have gene combinations y(11)y(11), y(11)y(11) and y(11)y(11) in the segregating populations from y(11)y(11) plants. the gene in question is responsible for chlorophyll synthesis. the y(11)y(11) plants have dark green leaves, y(11)y(11) are light green and y(11)y(11) plants are golden yellow. the heterozygous plants have dark green, yellow and dark green-y ...19734797661
inheritance of flavonol glycosides in soybeans. 19734798644
thiocarbamate sulfoxides: potent, selective, and biodegradable herbicides.sulfoxidation of thiocarbamates yields a new class of chemicals having increased herbicidal activity along with greater tolerance of corn and soybeans in greenhouse tests. however, their thermal stability is not favorable. these sulfoxides are intermediates in the mammalian metabolism of thiocarbamates, being formed by liver microsomal oxidases and cleaved in a system consisting of glutathione and a soluble enzyme from liver.20164821957
acid protease production by fungi used in soybean food fermentation.growth conditions for maximum protease production by rhizopus oligosporus, mucor dispersus, and actinomucor elegans, used in oriental food fermentations, were investigated. enzyme yields by all three fungi were higher in solid substrate fermentations than in submerged culture. the level of moisture in solid substrate must be at about 50 to 60%. very little growth of these fungi was noted when the moisture of substrate was below 35%, whereas many fungi including most storage fungi generally grow ...20144857353
minimum rates of insecticides on soybeans: mexican bean beetle, green clover worm, corn earworm and velvetbean caterpillar. 20164857577
effects of plant growth regulators on levels of phorate and aldicarb in soybeans. 20134857669
fatty acid composition of chicks fed full-fat soybeans. 19744858374
trypsin inhibitor content in some varieties of soya bean (glycine max l.) and sunflower seeds (helianthus annuus l.). 19744859576
infestations of phytophagous insects on soybeans following applications of du pont 1410 foliar sprays and other insecticides applied to the soil. 19725061818
biochemical and nutritional assessment of different varieties of soybeans. 19725062148
mellein and 4-hydroxymellein production by aspergillus ochraceus wilhelm.mellein and 4-hydroxymellein are isocoumarin compounds produced by aspergillus ochraceus wilhelm. they are structurally similar to the dihydroisocoumarin moiety of ochratoxin a, a toxic metabolite of the same fungus, and they possibly have similar biological properties. production of mellein and 4-hydroxymellein on synthetic media and natural solid substrates was determined. several carbon and nitrogen sources supported production of these metabolites in stationary culture. additional zinc and m ...19725064985
suppression of somatic crossing over in glycine max (l.) merrill by deoxyribose cytidine. 19725065252
fate of edc-ccl 4 (75:25) residues during milling and oil extraction of soybeans. 19725066159
collaborative study of the determination of aflatoxin in corn and soybeans. 19725066463
absorption, translocation, and metabolism of p-nitrophenyl-alpha, alpha, alpha-trifluoro-2-nitro-p-tolyl ether by soybeans. 20085103263
effect of cooked soybeans on performance, fatty acid composition and pork carcass characteristics. 19715106333
the utilization of unextracted raw and extruded full-fat soybeans by the chick. 19715106715
pelleting of soybean (glycine max) seeds with activated charcoal and humus. 19715108332
effects of certain systemic insecticides on growth and yield of soybeans. 19715166207
effect of feeding soybeans of formaldehyde-treated soybeans on lipid metabolism in ruminants. 19715167272
effect of azotobacter chroococcum and tryptophan on the inoculation of soybean (glycine max (l.) merril) with rhizobium japonicum. 19695271557
raw soybeans and cystine metabolism. 19655321077
residues of endrin and ddt in soybeans grown on soil treated with these compounds. 19695387692
ddt residues in tobacco and soybeans grown in soil treated with ddt. 19695387693
residues of ddt and endrin in peanuts and soybeans grown in soil containing these pesticides. 19695387694
the use of raw and extruded soybeans in layer diets. 19695387889
metabolism of the plant growth regulator 2,35-triiodobenzoic acid in soybeans. 19695388694
effect of raw soybeans on levels of protein and nucleic acids in the rat pancreas. 19695389757
separation of a mouse growth inhibitor in soybeans from trypsin inhibitors. 19705466150
effects of cowpea chlorotic mottle virus (soybean strain) on chemical composition of davis soybeans. 20005528923
purification and some properties of a non-haem iron protein from the bacteroids of soya-bean (glycine max merr) nodules.a non-haem iron protein was isolated from an extract of soya-bean nodule bacteroids by a procedure including protamine sulphate and heat precipitation followed by chromatography on deae-cellulose. the purified protein contains non-haem iron and acid-labile sulphur and exhibits a spectrum with a rather broad absorption shoulder in the region 380-440nm and a more prominent peak at 280nm. from sedimentation-velocity measurements an apparent s(20,w) value of 1.3s was calculated. the protein function ...19705529239
nutritional value of soybeans in the diet of depancreatized chickens. 19705530321
correlation analysis of criteria of symbiotic nitrogen. fixation by soybeans (glycine max merr.). 19705535969
further evidence for a dual, independent, activity against trypsin and alpha-chymotrypsin of inhibitor aa from soybeans. 19675624045
effect of raw and heated soybeans on gain, nutrient digestibility, plasma amino acids and other blood constituents of growing swine. 19675624670
biochemical changes in the pancreases of chicks fed raw soybeans and soybean meal. 19675625405
analysis of oil content of soybeans by wide-line nmr. 19675625862
iron in relation to aconitate hydratase activity in glycine max. merr. 19685646051
study of absorption, translocation, and residue properties of 2,3,5-triiodobenzoic acid in field-grown soybeans. 19685690544
monitoring for chlorinated hydrocarbon insecticide residues in soybeans--1966. plant pest control division, agricultural research service u.s. department of agriculture. 19685693989
isolation and purification of an antifungal principle from infected soybeans. 19685753113
hormonal control of cell proliferation and xylem differentiation in cultured tissues of glycine max var. biloxi.the relationship between tracheary element differentiation, cell proliferation and growth hormones was examined in agar-grown soybean callus. the time course of cell division and tracheary element formation in tissues grown on a medium containing 5 x 10(-7)m kinetin and 10(-5)m naa was determined by means of maceration technique. after a slight lag period, a logarithmic increase in cell number was observed through the twelfth day of the culture period. cell numbers increased at a considerably sl ...19695816361
herbicide metabolism: n-glycoside of amiben isolated from soybean plants.the n-glycoside of the herbicide 3-amino-2, 5-dichlorobenzoic acid (amiben) was synthesized in the laboratory. the compound was chromatographically identical with a conjugate of amiben isolated from soybean plants, glycine max (l.) merr. the seeds of a tolerant plant, soybean, and of a susceptible one, barley (hordeum vulgare l.), were incubated in amiben labeled with carbon-14, and the seedlings were extracted. essentially all the radioactivity in soybean was present as a compound corresponding ...19655891144
relationship of fractons of soybeans and a crystalline soybean trypsin inhibitor to the effects of feeding unheated soybean meal to chicks. 19665948027
trypsin inhibition, hemagglutination, and intraperitoneal toxicity in extracts of phaseolus vulgaris and glycine max. 19665949799
simultaneous p32- and c14-labeling of phospholipids by germinating soybeans.doubly labeled ((32)p and (14)c) phospholipids of high specific activity are obtained by incorporating the labels during germination of soybeans.19665965303
effect of soybean meal, raw soybeans, corn gluten feed, and urea on the concentration of rumen fluid components at intervals after feeding. 19676069995
[catalytic concentration, multiple forms, and lectin affinity of microsomal enzymes from human tissues: lectins as reagents, ii (author's transl)].we estimated the concentrations, multiple forms, and lectin binding of five microsomal enzymes in particle free extracts from human kidney, pancreas, jejunal mucosa, and normal and cancerous liver. while arylesterase markedly reacted only with concanavalin a, arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase were intensely precipitated by lectins from ricinus communis 120, canavalia ensiformis, triticum vulgare and phaseolus vulgaris s. agglutinins from glycine max ...19816120206
four isoforms of glutamine synthetase in light-grown soybeans.glutamine synthetase (gs) activity recovered from linear sucrose gradients was associated with the cytosol of cells isolated from etiolated soybean hypocotyls whereas light-grown tissue contained increased gs activity localized in both the cytosol and chloroplasts. deae-cellulose chromatography indicated two gs isoforms in etiolated hypocotyls whereas light-grown hypocotyls and primary leaves contained four isoforms. only one gs isoform was recovered from both etiolated and light-grown cotyledon ...19836136278
aflatoxin - induced alteration in the levels of membrane chemical of subcellular organelles isolated from excised, incubated glycine max, cv. "essex" roots. i. non-enriched organelles. 19816166863
purification and characterization of mrna from soybean seeds. identification of glycinin and beta-conglycinin precursors.poly(a)-rich rna was isolated from developing soybean seeds (glycine max (l.) merr.) and fractionated on linear log sucrose gradients. two major fractions sedimenting at 18 s and 20 s were separated and then purified by further sucrose gradient fractionation. both fractions were active as messengers when added to a rabbit reticulocyte lysate protein synthesis system. the 18 s fraction caused proteins migrating primarily to the 60,000-dalton region of a sodium dodecyl sulfate gel to be produced, ...19816167584
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