Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| signaling by glutamate dehydrogenase in response to pesticide treatment and nitrogen fertilization of peanut (arachis hypogaea l.). | the responses of glutamate dehydrogenase (gdh) to nh(4)(+) and herbicides offer a new approach for probing the effects of nh(4)(+)-pesticide interactions at the whole-plant level. although pesticides and fertilizers have greatly enhanced food production, their combined biochemical effects are not known in detail. peanut plants were treated with different rates of basagran (3-(1-methylethyl)-1h-2,1,3-benzothiadiazin-4(3h)-one 2,2-dioxide), bravo 720 (tetrachloroiso-phthalonitrile), and sevin xlr ... | 1999 | 10552654 |
| phylogeny and diversity of bradyrhizobium strains isolated from the root nodules of peanut (arachis hypogaea) in sichuan, china. | twenty-two rhizobial strains isolated from the root nodules of two chinese peanut cultivars (arachis hypogaea l. tianfu no. 3 and a local cultivar) growing at four different sites in the sichuan province, southwest china, were characterized by growth rate, rep-pcr, pcr-rflp of 16s rdna, partial sequencing of ribosomal genes, and fatty acid-methyl ester analysis (fame), and compared with strains representing bradyrhizobium japanicum, b. elkanii and other unclassified bradyrhizobium sp. all peanut ... | 1999 | 10553290 |
| cross-reaction of chalcone synthase and stilbene synthase overexpressed in escherichia coli. | chalcone synthase (chs) and stilbene synthase (sts) are related plant polyketide synthases belonging to the chs superfamily. chs and sts catalyze common condensation reactions of p-coumaroyl-coa and three c(2)-units from malonyl-coa but different cyclization reactions to produce naringenin chalcone and resveratrol, respectively. using purified pueraria lobata chs and arachis hypogaea sts overexpressed in escherichia coli, bisnoryangonin (bny, the derailed lactone after two condensations) and p-c ... | 1999 | 10556516 |
| indirect competitive elisa for determination of traces of peanut (arachis hypogaea l.) protein in complex food matrices. | an indirect competitive elisa was developed allowing the detection of hidden peanut protein residues down to 2 ppm (micorgrams per gram) in various foods. the high-titer, peanut-specific polyclonal antiserum used recognized potentially allergenic proteins in both native and roasted peanuts. in the absence of a food matrix, extractable protein from roasted peanuts was detected at 104 +/- 13%. from various food items, peanut protein at > or =13 ppm was recovered between 84 and 126%, and at 2 ppm o ... | 1999 | 10563939 |
| lectin histochemistry of dog major and minor salivary glands. | the distribution of different carbohydrates in dog major and minor salivary glands was investigated using a peroxidase-labelled avidin-biotin method to demonstrate binding of six lectins (canavalia ensiformis agglutinin [con a], dolichos biflorus agglutinin [dba], arachis hypogaea (peanut) agglutinin [pna], glycine max agglutinin [sba], tetragonolobus purpurea agglutinin [tgp] and wheat germ agglutinin [wga]). with pna, there was only weak staining in serous acini of parotid glands. other lectin ... | 1999 | 10596406 |
| influence of palm olein on protein utilisation in the growing rat. | in view of our preliminary findings that refined palm oil (rpol) enhanced protein utilisation, and because of the growing importance of palm oil in human diets, two separate studies were conducted. the first study aimed to investigate the effect of the palm oil source and fraction on protein utilisation, as determined by net protein utilization (npu) in growing rats. the second study aimed to investigate the influence of the dietary concentration of one particular palm oil fraction, refined palm ... | 1999 | 10616647 |
| synthesis of bioadhesive lectin-hpma copolymer-cyclosporin conjugates. | an amino group containing cyclosporin a (csa) derivative has been synthesized and conjugated to n-(2-hydroxypropyl)methacrylamide (hpma) copolymer via an aromatic azo bond, which can be specifically cleaved by azoreductase activity in colon to release the drug for the treatment of colon diseases. lectins, peanut (arachis hypogea) agglutinin (pna) and wheat germ agglutinin (wga), have been conjugated to hpma copolymer-csa derivative conjugates (pcsa), respectively, to give bioadhesive conjugates. ... | 2000 | 10639078 |
| location, substructure, and composition of basal laminar drusen compared with drusen associated with aging and age-related macular degeneration. | to determine whether basal laminar drusen differ in their location, ultrastructure, or composition from drusen associated with aging and age-related macular degeneration. | 2000 | 10682974 |
| endothelial glycoconjugates: a comparative lectin study of the brain, retina and myocardium. | there is evidence that the endothelial cell (ec) glycocalyx is a significant determinant of vascular permeability, acting as a charge-size filter to permeant molecules. we have therefore examined its oligosaccharide composition in 3 classes of microvessel with differing permeabilities. ec in rat brain, retina and myocardium were labelled with a panel of lectins and subjected to a semiquantitative analysis. surprisingly, no substantial differences were evident for any lectin labelling between the ... | 2000 | 10697288 |
| analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system. | previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ... | 2000 | 10758890 |
| constitutive accumulation of a resveratrol-glucoside in transgenic alfalfa increases resistance to phoma medicaginis. | alfalfa (medicago sativa) was transformed with a peanut (arachis hypogaea) cdna encoding resveratrol synthase (rs) transcriptionally regulated by an enhanced cauliflower mosaic virus (camv) 35s promoter. transgenic plants accumulated a new compound, not present in wild-type or vector-transformed alfalfa, that was identified as trans-resveratrol-3-o-beta-d-glucopyranoside (rgluc) by high-pressure liquid chromatography (hplc), uv, 1h- and 13c-nuclear magnetic resonance (nmr) analyses. rgluc concen ... | 2000 | 10796021 |
| a peanut seed lipoxygenase responsive to aspergillus colonization. | several lines of evidence have indicated that lipoxygenase enzymes (lox) and their products, especially 9s- and 13s-hydroperoxy fatty acids, could play a role in the aspergillus/seed interaction. both hydroperoxides exhibit sporogenic effects on aspergillus spp. (calvo, a., hinze, l., gardner, h.w. and keller, n.p. 1999. appl. environ. microbiol. 65: 3668-3673) and differentially modulate aflatoxin pathway gene transcription (burow, g.b., nesbitt, t.c., dunlap, j. and keller, n.p. 1997. mol. pla ... | 2000 | 10809442 |
| double electrophoretic separation and lectin analyses of the component chains of secretory immunoglobulin a from human saliva. | a new method is presented for the separation of secretory immunoglobulin a (siga) from salivary samples. salivary proteins (from parotid or stimulated whole mouth saliva) were precipitated with methanol to concentrate siga from salivary samples whilst removing other salivary proteins. siga purified from breast milk and salivary proteins was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) under nonreducing conditions. following completion of electrophoresis t ... | 2000 | 10832871 |
| interspecific variation of zona pellucida glycoconjugates in several species of marsupial. | the zona pellucida glycoconjugate content of several marsupial species was investigated using differential lectin histochemistry. ovaries from fat-tailed dunnarts, a southern brown bandicoot, grey short-tailed opossums, brushtail possums, ringtail possums, koalas and eastern grey kangaroos were fixed, embedded in paraffin wax, sectioned and stained with ten fluorescein isothiocyanate-conjugated lectins. sections were also incubated with either neuraminidase or saponified, respectively, before in ... | 2000 | 10864820 |
| genome size variation in arachis hypogaea and a. monticola re-evaluated. | genome size variation within species is a frequently reported, but still a controversial problem. in the present study, we re-evaluated recently published feulgen densitometric data on genome size and its infraspecific variation in arachis hypogaea, and also conducted measurements in one accession of its wild relative a. monticola. the methods applied were propidium iodide flow cytometry and feulgen densitometry using pisum sativum as an internal standard. the 2c dna contents previously publishe ... | 2000 | 10902707 |
| coculture of chicken thrombocytes and monocytes: morphological changes and lectin binding. | chicken leukocytes were separated from blood on a percoll cushion following adherence on coverslips, resulting in a coculture of thrombocytes and monocytes. this system was characterized by light microscopy, scanning and transmission electron microscopy, by lectin binding and actin localization in thrombocytes. during the first 24 hours nuclear condensation, cytoplasmic shrinkage, detachment and death of thrombocytes were observed. in contrast, monocytes gradually increased their spreading capac ... | 1998 | 10904525 |
| the high oleate trait in the cultivated peanut [arachis hypogaea l.]. i. isolation and characterization of two genes encoding microsomal oleoyl-pc desaturases. | plant oils rich in oleate are considered superior products compared to oils rich in polyunsaturated fatty acids. peanut (arachis hypogaea l.) is one of the major oilseed crops, and high oleate mutant varieties with as much as 85% oleate have been reported. we examined the possibility that this mutant phenotype resulted from reduction in the activity or the transcript level of microsomal oleoyl-pc desaturase. two independently generated high oleate mutants, m2-225 and 8-2122, and their partially ... | 2000 | 10905347 |
| the high oleate trait in the cultivated peanut [arachis hypogaea l]. ii. molecular basis and genetics of the trait. | a peanut variety with high oleate content has previously been described. when this high oleate variety was used in breeding crosses, the f2 segregation ratio of high oleate to normal oleate progeny was 3:1 or 15:1 depending on the normal oleate varieties used in the crosses. these data suggested that the high oleate trait is controlled by two recessive genes, and some peanut varieties differ from the high oleate variety by mutations in one gene, while others differ by mutations in two genes. the ... | 2000 | 10905348 |
| lithium nephrotoxicity: a progressive combined glomerular and tubulointerstitial nephropathy. | this study examines the clinical features, pathologic findings, and outcome of 24 patients with biopsy-proven lithium toxicity. the patient population was 50% male, 87.5% caucasian, and had a mean age of 42.5 yr (range, 26 to 57). mean duration of lithium therapy for bipolar disorder was 13.6 yr (range, 2 to 25). all patients were biopsied for renal insufficiency (mean serum creatinine 2.8 mg/dl; range, 1.3 to 8.0), with associated proteinuria >1.0 g/d in 41.7%. nephrotic proteinuria (>3.0 g/d) ... | 2000 | 10906157 |
| glycosylation of the cationic peanut peroxidase gene expressed in transgenic tobacco. | the major cationic peanut (arachis hypogaea) peroxidase, secreted into the extracellular space, is a glycoprotein with three n-linked glycans (polysaccharides) which are connected to the peptide backbone at asn-60, asn-144 and asn-185. in this report, a c-terminal histidine-tagged cationic peanut peroxidase gene was expressed in transgenic tobacco (nicotiana tabacum). tissue of the transgenic tobacco was cultured in suspension culture and the his-tagged peroxidase was purified in large quantitie ... | 2000 | 10908805 |
| improved plant regeneration from cultured leaf segments in peanut (arachis hypogaea l.) by limited exposure to thidiazuron. | bud primordia were induced from leaf segments, which were harvested from young seedlings of spanish type peanut (arachis hypogaea l. cv. chico), on 0.8% agar-solidified medium containing murashige and skoog (ms) basal salts supplemented with b5 vitamins, 1 mg/l naa and various cytokinins such as benzyladenine (ba), isopentenyladenine (2ip), kinetin (kin), chloropyridylphenylurea (4pu), thidiazuron (tdz), zeatin (ztn) in different concentrations. among the cytokinins tested, tdz was found to be t ... | 2000 | 10936523 |
| effects of short episodes of heat stress on flower production and fruit-set of groundnut (arachis hypogaea l.). | groundnuts (arachis hypogaea l.) are an important crop of the semi-arid tropics where they are often exposed to maximum temperatures of > 40 degrees c for short periods during the growing season. the objectives of this study were to determine: (i) the effects of short periods of exposure to high temperature on flower production (fn), the proportion of flowers forming fruits (fruit-set) and the number of pegs and pods per plant (rnt); (ii) whether fruit-set is affected by high temperature during ... | 2000 | 10938870 |
| a comparison study on allergen components between korean (arachis fastigiata shinpung) and american peanut (arachis hypogaea runner). | the prevalence of peanut allergy in korea is lower than in america. peanut extract allergens between the two countries have not been standardized. this study was performed to compare the allergenicity of raw korean and american peanuts with that of roasted peanuts. we prepared the peanut extracts in korean raw (kp) and roasted peanuts (krp), and also in american raw (ap) and roasted (arp) peanuts. we compared the peanut extract allergens of kp, krp, ap and arp in vitro with sodium dodecyl sulfat ... | 2000 | 10983685 |
| identification of dna polymorphism in cultivated groundnut using random amplified polymorphic dna (rapd) assay. | construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. except for the recent studies from two laboratories, most of the previous studies have shown little or no dna polymorphism in cultivated groundnut (arachis hypogaea l.). in the present study, 70 selected genotypes, representing variability for several morphological, physiological, and other characters, were studied for polymorphism employing random amplified polymorphic dna ... | 2000 | 10984178 |
| a lectin-histochemical study on the seminiferous epithelium of the northern smooth-tailed tree shrew (dendrogale murina) and the java tree shrew (tupaia javanica). | lectin-binding patterns in the testes of the northern smooth-tailed tree shrew, dendrogale murina and java tree shrew, tupaia javanica were studied by light microscopy and compared the data with those of the common tree shrew. four lectins (pna, sba, bpa and gs-ii) were used in this study. peanut (arachis hypogaea) agglutinin (pna), soybean (glycine max) agglutinin (sba) and bauhinia purpurea agglutinin (bpa) showed a strong reaction in the acrosomal region from golgi to acrosome-phase spermatid ... | 2000 | 10998938 |
| an efficient method for the production of transgenic plants of peanut (arachis hypogaea l.) through agrobacterium tumefaciens-mediated genetic transformation. | cotyledon explants from mature peanut seeds (arachis hypogaea l.) were optimized to obtain adventitious shoot buds with high frequencies (>90%). efficient transformation of these cotyledons by using agrobacterium tumefaciens strain c58 carrying neomycin phosphotransferase ii (nptii) and ß-glucuronidase (gus; uida), or coat protein gene of the indian peanut clump virus (ipcvcp) and nptii on binary vectors (pbi121; prokii:ipcvcp) led to the production of a large percentage (55%) of transgenic plan ... | 2000 | 11011088 |
| reaction of various lectins to mucin derived from the different layers of rat gastric mucosa: comparison of enzyme-linked lectin binding assay with lectin histochemistry. | the purpose of this study was to demonstrate that the histochemical staining reactivities of lectins in rat stomach actually represent the gastric mucins, and to estimate the utility of the lectins for mucin histochemistry. in this paper, the lectin histochemistry was compared with an enzyme-linked lectin binding assay (ella) of the mucins derived from distinct regions and layers of the sprague-dawley rat stomach and it was examined to determine the definite binding and problematic binding of th ... | 2000 | 11041246 |
| identification of perivitelline n-linked glycans as mediators of sperm-egg interaction in chickens. | this study demonstrates that carbohydrates play an essential role in sperm-egg interactions in birds. sperm-egg interaction was measured in vitro as the ability of spermatozoa to hydrolyse a small hole in the inner perivitelline layer, the equivalent of the mammalian zona pellucida. preincubation with triticum vulgaris lectin (wga) and succinyl-wga (s-wga) at 10 microgram ml(-1) resulted in complete inhibition of sperm-egg interaction, whereas at the same concentration a range of other lectins ( ... | 2000 | 11058456 |
| chemoenzymatic synthesis of glycopolypeptides carrying alpha-neu5ac-(2-->3)-beta-d-gal-(1-->3)-alpha-d-galnac, beta-d-gal-(1-->3)-alpha-d-galnac, and related compounds and analysis of their specific interactions with lectins. | glycopolypeptide (1) carrying the beta-d-gal-(1-->3)-alpha-d-galnac unit as a kind model of asialo-type mucin was synthesized through three steps: enzymatic synthesis of p-nitrophenyl disaccharide glycoside, reduction of the p-nitrophenyl group, and coupling of the amino group with the carboxyl group of poly(l-glutamic acid)s (pga). in a similar manner, glycopolypeptides (2-7) carrying beta-d-gal-(1-->3)-beta-d-galnac, beta-d-gal-(1-->3)-beta-d-glcnac, beta-d-gal-(1-->6)-alpha-d-galnac, beta-d-g ... | 2000 | 11097173 |
| use of a single method in the extraction of the seed storage globulins from several legume species. application to analyse structural comparisons within the major classes of globulins. | in this study, a single, improved methodology was used to extract, fractionate and purify the 11s (legumin-type or related to the alpha-conglutin from lupinus albus l.), 7s (vicilin-type or related to the beta-conglutin from l. albus) and 2s (related to the gamma-conglutin from l. albus) families of proteins from eight legume species: l. albus, glycine max (l.) merr., pisum sativum l., vicia faba l., cicer arietinum l., phaseolus vulgaris l., lens culinaris med. and arachis hypogaea l. the sedim ... | 2000 | 11103299 |
| opposite effects on human colon cancer cell proliferation of two dietary thomsen-friedenreich antigen-binding lectins. | increased cell surface expression of the thomsen-friedenreich antigen (tf antigen, galbeta1-3galnacalpha-) is a common feature in malignant and pre-malignant epithelia. our previous studies have shown that dietary tf-binding lectins from peanut (arachis hypogea) and edible mushroom (agaricus bisporus) produce marked but different effects on human intestinal epithelial cell proliferation. this study investigates the proliferative effects of the other two known dietary tf-binding lectins: jacalin ... | 2001 | 11169464 |
| analysis of glycoconjugate patterns of normal and hormone-induced dysplastic noble rat prostates, and an androgen-independent noble rat prostate tumor, by lectin histochemistry and protein blotting. | alteration of the expression of glycoconjugates is frequently observed in tumors. however, studies on the changes of cellular glycosylation in the early premalignant stage of prostate carcinogenesis are scarce. | 2001 | 11170128 |
| molecular properties of the oleoyl-phosphatidylcholine desaturase from arachis hypogaea l. | deficiencies in the activity of the microsomal oleoyl phosphatidylcholine (oleoyl-pc) desaturase from peanuts are the basis of the high oleate oil. mutation of aspartate-150 to asparagine and the attendant decrease in activity, together with the loss in expression of the higher activity transcript, was the molecular basis of the high oleate trait. the ability of oleoyl-pc desaturase to desaturate palmitoleate, oleate and 10(z) nonadecenoate to methylene-interrupted products was not consistent wi ... | 2000 | 11171148 |
| segmental expression of somatostatin receptor subtypes sst(1) and sst(2) in tubules and glomeruli of human kidney. | somatostatin is known to modulate mesangial and tubular cell function and growth, but the somatostatin receptor (sst) subtypes responsible for these effects have not been defined. there are at least five different sst receptor subtypes (sst(1)-sst(5)). we used rt-pcr to demonstrate that normal human kidney consistently expresses mrna for sst(1) and sst(2) (9 of 9 donors). some donors expressed sst(4) or sst(5) mrna, but none expressed sst(3) mrna. expression of sst(1) and sst(2) was further asse ... | 2001 | 11181407 |
| mineral content of insect infested stored legumes treated with edible oils. | mineral content of three insect (pulse beetle, callosobruchus chinensis l.) infested legumes viz. chick pea, mung pea and pigeon pea stored for six months and treated with three edible oils viz. groundnut, mustard and coconut oil has been studied. with increase in storage period significant increase in calcium, phosphorus and iron content of untreated legumes was observed. after three months of storage slight increase in three minerals was observed in the legumes treated with coconut oil which c ... | 2000 | 11190836 |
| hypersialylated macromolecular serum immunoglobulin a1 in type 2 diabetes mellitus. | the origin of the elevation of serum immunoglobulin a1 (iga1) in type 2 diabetes mellitus (dm) is unsettled. the aim of this study was to address the carbohydrate changes of serum iga1 from patients with type 2 diabetes mellitus, as a possible cause of the elevation. | 2001 | 11239513 |
| genetic diversity of bradyrhizobium strains isolated from arachis hypogaea. | rhizobia are used exclusively in agricultural systems for enhancing the ability of legumes to fix atmospheric nitrogen. knowledge about the indigenous population is necessary for the selection and application of inoculant strains. in this study, we have assessed the genetic diversity of bradyrhizobium strains isolated from the host plant, arachis hypogaea along the coastline of tamil nadu. different populations collected from varying environmental conditions were analysed for salt and ph toleran ... | 2001 | 11261490 |
| identification, purification, and characterization of monoacylglycerol acyltransferase from developing peanut cotyledons. | biosynthesis of diacylglycerols in plants occurs mainly through the acylation of lysophosphatidic acid in the microsomal membranes. here we describe the first identification of diacylglycerol biosynthetic activity in the soluble fraction of developing oilseeds. this activity was naf-insensitive and acyl-coa-dependent. diacylglycerol formation was catalyzed by monoacylglycerol (mag) acyltransferase (ec ) that transferred an acyl moiety from acyl-coa to mag. the enzyme was purified by successive c ... | 2001 | 11283027 |
| measurement and modeling of diclosulam runoff under the influence of simulated severe rainfall. | a runoff study was conducted near tifton, ga to measure the losses of water, sediment, and diclosulam (n-(2,6-dichlorophenyl)-5-ethoxy-7-fluoro-[1,2,4]triazolo-[1,5c]-pyrimidine- 2-sulfonamide), a new broadleaf herbicide, under a 50-mm-in-3-h simulated rainfall event on three separate 0.05-ha plots. results of a runoff study were used to validate the pesticide root zone model (przm, v. 3.12) using field-measured soil, chemical, and weather inputs. the model-predicted edge-of-field diclosulam loa ... | 2012 | 11285917 |
| transformation of peanut (arachis hypogaea l.) with tobacco chitinase gene: variable response of transformants to leaf spot disease. | fertile transgenic plants of peanut (arachis hypogaea l.) cv. tmv-2 expressing tobacco chitinase and neomycin phosphotransferase (npt ii) genes were generated using an agrobacterium tumefaciens (lba4404/pbi121-pbtex)-mediated transformation system. a tissue culture-independent method wherein embryo in the mature seed is inoculated and reared into single plant transformant was used for transformation. southern blot analysis of genomic dna isolated from t(0) transformants and progeny plants (t(1)) ... | 2001 | 11297785 |
| the hydrophobic character of peanut (arachis hypogaea) isoagglutinins. | peanut seed lectin (pna) is widely used to identify tumor-specific antigens on the eukaryotic cell surface. in this work pna was purified by affinity chromatography, using a column containing glutaraldehyde-treated human erythrocytes, whereas pna isoforms were purified by hydrophobic interaction chromatography using phenyl-sepharose. the affinity-purified pna and its isoforms consist of four equal subunits of 24.5 kda each, all of which agglutinated human sialidase-treated erythrocytes equally w ... | 2000 | 11312799 |
| peanut (arachis hypogaea) lectin recognizes alpha-linked galactose, but not n-acetyl lactosamine in n-linked oligosaccharide terminals. | peanut (arachis hypogaea) agglutinin (pna) is extensively used as tumour marker as it strongly recognises the cancer specific t antigen (galbeta1-->3galnac-), but not its sialylated version. however, an additional specificity towards galbeta1-->4glcnac (lacnac), which is not tumour specific, had been attributed to pna. for correct interpretation of lectin histochemical results we examined pna sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactos ... | 2001 | 11325423 |
| hypogin, a novel antifungal peptide from peanuts with sequence similarity to peanut allergen. | a protein designated hypogin, with a prominent suppressive action on the growth of the fungi mycosphaerella arachidicola, fusarium oxysporum and coprinus comatus, was isolated from seeds of the peanut arachis hypogaea. the protein inhibited human immunodeficiency virus (hiv) reverse transcriptase and enzymes associated with hiv infection including alpha-glucosidase and beta-glucosidase. the proliferative response of mouse splenocytes was attenuated in the presence of the protein. the protein exh ... | 2001 | 11328490 |
| parental effects in the inheritance of nonnodulation in peanut. | a nonnodulating line (m4-2) and three normal nodulating lines (uf 487a, pi 262090, and florunner) of peanut (arachis hypogaea l.) were crossed in full diallel to investigate the inheritance of nodulation. data from f1, f2, f3, f1bc1, and f2bc1 generations indicated that three genes control nodulation at three independent loci, with nodulation being a product of two genes and inhibited by a third gene when it is dominant and the others are homozygous recessive. a genetic model has been proposed t ... | 2015 | 11336237 |
| the effects of the site-directed removal of n-glycosylation from cationic peanut peroxidase on its function. | peanut peroxidase has been diffracted. the location of its heme and calcium moieties have been shown and their role demonstrated. however, the structure and role of its glycans is only now being elucidated. the role of three n-linked complex glycans on cationic peroxidase (cprx) of peanut (arachis hypogaea l cv. valencia), as expressed by prxpnc1 in transgenic tobacco, was analyzed by site-directed replacement of each of the three glycosylation sites, n-60, n-144, and n-185 with q, individually. ... | 2001 | 11360996 |
| o-glycosylation in sprouting neurons in alzheimer disease, indicating reactive plasticity. | reactive plasticity, including axonal and dendritic sprouting and reactive synaptogenesis, has been proposed to contribute to the pathogenesis of several neurological disorders. this work was aimed at identifying the possible role of protein glycosylation in the brain from patients with alzheimer disease (ad), using lectin histochemistry, as determinants of reactive plasticity. results indicate an increase in the production of cryptic o-glycosidically linked proteins (neuacalpha2,6 galbeta1,3gal ... | 2001 | 11379819 |
| endoplasmic oleoyl-pc desaturase references the second double bond. | the regiospecificity for the gene product of fad2,(1) the microsomal oleoyl-pc desaturase from higher plants, differs from some previous suggestions. rather than only referencing the carboxyl group (a delta(12) desaturase) or the methyl terminus (an omega-6 desaturase), this desaturase locates the second double bond in its substrates by first referencing the existing double bond. this specificity was demonstrated for the oleoyl-pc desaturase cdna from the developing seeds of peanut (arachis hypo ... | 2001 | 11397429 |
| patient-tailored cloning of allergens by phage display: peanut (arachis hypogaea) profilin, a food allergen derived from a rare mrna. | a peanut cdna phage surface display library was constructed and screened for the presence of ige-binding proteins. we used a serum from a peanut-sensitized individual with a low specific ige level to peanut extract and suffering from mild symptoms after peanut ingestion. a total of 10(11) cdna clones were screened by affinity selection towards serum ige immobilized to solid-phase supports. after five rounds of selective enrichment, sequence determination of 25 inserts derived from different clon ... | 2001 | 11419720 |
| novel polyketides synthesized with a higher plant stilbene synthase. | the physiological function of the stilbene synthase (sts) from groundnut (arachis hypogaea) is the formation of resveratrol. the enzyme uses 4-coumaroyl-coa, performs three condensations with malonyl-coa, and folds the resulting tetraketide into a new aromatic ring system. we investigated the capacity for building novel and unusual polyketides from alternative substrates. three types of products were obtained: (a) complete reaction (stilbene-type), (b) three condensations without formation of an ... | 2001 | 11432743 |
| adenovirus-mediated expression of pig alpha(1, 3) galactosyltransferase reconstructs gal alpha(1, 3) gal epitope on the surface of human tumor cells. | gal alpha(1, 3) gal (gal epitope) is a carbohydrate epitope and synthesized in large amount by alpha(1, 3) galactosyltransferase [alpha(1, 3) gt] enzyme on the cells of lower mammalian animals such as pigs and mice. human has no gal epitope due to the inactivation of alpha(1, 3) gt gene but produces a large amount of antibodies (anti-gal) which recognize gal alpha(1, 3) gal structures specifically. in this study, a replication-deficient recombinant adenoviral vector ad5sgt containing pig alpha(1 ... | 2001 | 11453543 |
| transgenic peanut plants obtained by particle bombardment via somatic embryogenesis regeneration system. | after pre-culture and treatment of osmosis, cotyledons of immature peanut (arachis hypogaea l.) zygotic embryos were transformed via particle bombardment with a plasmid containing a chimeric hph gene conferring resistance to hygromycin and a chimeric intron-gus gene. selection for hygromycin resistant calluses and somatic embryos was initiated at 10th d post-bombardment on medium containing 10-25 mg/l hygromycin. under continuous selection, hygromycin resistant plantlets were regenerated from so ... | 2001 | 11453548 |
| synthesis of n,n'-bis(acrylamido)acetic acid-based t-antigen glycodendrimers and their mouse monoclonal igg antibody binding properties. | novel glycodendrimers based on n,n'-bis(acrylamido)acetic acid core with valencies between two and six were synthesized. the breast cancer-associated t-antigen carbohydrate marker, (beta-gal-(1-3)-alpha-galnac-or), was then conjugated by (i) 1,4-conjugate addition of thiolated t-antigen to the n-acrylamido dendritic cores and by (ii) amide bond formation between an acid derivative of the t-antigen and the polyamino dendrimers. the protein-binding ability of these new glycodendrimers was fully de ... | 2001 | 11456798 |
| genotypic differences in the presence of hairs on roots and gynophores of peanuts (arachis hypogaea l.) and their significance for phosphorus uptake. | root hairs substantially increase the surface area of plant roots with positive effects for phosphorus (p) uptake, but the ability of peanuts to form root hairs has been questioned. the aim was to examine hair development on roots and gynophores of a variety of peanut genotypes and to relate genotypic differences in hair formation to differences in p uptake. five out of eighteen genotypes completely lacked hairs on both organs whereas others consistently developed hairs on roots and gynophores, ... | 2001 | 11479336 |
| microcosm studies on the persistence of peanut inoculant in acid soil and its interaction with indigenous soil micro-organisms. | the effect of a native peanut (arachis hypogaea) microsymbiont strain (streptomycin resistant mutant) introduced into acid soil on the indigenous micro-organism population as well as the survival of this isolate was studied. although the isolate survived at a fairly low population level after the 3 month period of soil incubation, it maintained its antibiotic resistance. slight changes in the total bacteria and fungi soil populations as a result of this inoculation were also detected. | 2001 | 11491523 |
| changes in auxin patterns in developing gynophores of the peanut plant (arachis hypogaea l.). | the peanut plant (arachis hypogaea l.) produces flowers aerially, but buries the recently fertilized ovules in the soil in order for the fruit and seeds to mature underground. the organ that carries the seeds into the soil is called the gynophore. the growth of the peanut gynophore is regulated primarily by indole-3-acetic acid (iaa). a monoclonal antibody raised against iaa was used to successfully detect and localize this growth substance in the tissues of developing peanut gynophores. five di ... | 1999 | 11541548 |
| a recirculating hydroponic system for studying peanut (arachis hypogaea l.). | peanut (arachis hypogaea l.) plants were grown hydroponically, using continuously recirculating nutrient solution. two culture tray designs were tested; one tray design used only nutrient solution, while the other used a sphagnum-filled pod development compartment just beneath the cover and above the nutrient solution. both trays were fitted with slotted covers to allow developing gynophores to reach the root zone. peanut seed yields averaged 350 gm-2 dry mass, regardless of tray design, suggest ... | 1998 | 11541799 |
| growth rates and auxin effects in graviresponding gynophores of the peanut, arachis hypogaea (fabaceae). | the gynophore of the peanut plant (arachis hypogaea) is a specialized organ that carries and buries the fertilized ovules into the soil in order for seed and fruit development to occur underground. the rates of growth of vertically and horizontally oriented gynophores were measured using a time-lapse video imaging system. we found that the region of maximum extension growth due to elongation (termed the central elongation zone) is located on average at 2-5 mm from the tip. in the first 0-4 h aft ... | 1998 | 11541946 |
| the role of amyloplasts during gravity perception in gynophores of the peanut plant (arachis hypogaea). | gravitropic perception and response are essential for the completion of the reproductive life cycle of the peanut plant (arachis hypogaea l.). the developing seeds are buried in the soil by a specialized organ, the gynophore, allowing the fruit to mature underground. controversy exists about the site of graviperception in the gynophore: previous workers suggested that the intercalary meristem was the zone where gravity was perceived. taking the starch statolith hypothesis for graviperception as ... | 1999 | 11542658 |
| thomsen-friedenreich (t) antigen: a possible tool for differentiating sebaceous carcinoma from its simulators. | the thomsen-friedenreich (t) antigen is a cryptic glycoprotein, referred to as tumor antigen or cancer-associated antigen because it is absent or masked by some carbohydrates in normal tissues, but present in many human cancers. the latter include gastrointestinal, lung, pancreatic, mammary, and some ovarian carcinomas. cancer cells frequently undergo incomplete glycosylation resulting in the appearance of precursor structures that normally would be absent like the case with the t antigen. t ant ... | 2001 | 11556753 |
| final report on the safety assessment of peanut (arachis hypogaea) oil, hydrogenated peanut oil, peanut acid, peanut glycerides, and peanut (arachis hypogaea) flour. | peanut (arachis hypogaea) oil is the refined fixed oil obtained from the seed kernels of arachis hypogaea. hydrogenated peanut oil, peanut acid, and peanut glycerides are all derived from peanut oil. peanut flour is a powder obtained by the grinding of peanuts. the oils and glycerides function in cosmetic formulations as skin-conditioning agents. the acid functions as a surfactant-cleansing agent, and the flour functions as an abrasive, bulking agent and/or viscosity-increasing agent. in 1998, o ... | 2001 | 11558642 |
| transmission genetics of chromatin from a synthetic amphidiploid to cultivated peanut (arachis hypogaea l.). broadening the gene pool of a monophyletic polyploid species. | polyploidy creates severe genetic bottlenecks, contributing to the genetic vulnerability of leading crops. cultivated peanut is thought to be of monophyletic origin, harboring relatively little genetic diversity. to introduce variability from diploid wild species into tetraploid cultivated arachis hypogaea, a synthetic amphidiploid [[a. batizocoi k9484 x (a. cardenasii gkp10017 x a. diogoi gkp10602)](4x)] was used as donor parent to generate a backcross population of 78 progeny. three hundred se ... | 2001 | 11606556 |
| rapd and issr fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (arachis hypogaea) cultivars and wild species. | abstract: twenty-one random and 29 ssr primers were used to assess genetic variation and interrelationships among subspecies and botanical varieties of cultivated peanut, arachis hypogaea (2n = 4x = 40), and phylogenetic relationships among cultivated peanut and wild species of the genus arachis. in contrast with the previous generalization that peanut accessions lack genetic variation, both random and ssr primers revealed 42.7 and 54.4% polymorphism, respectively, among 220 and 124 genetic loci ... | 2001 | 11681599 |
| genome size in arachis duranensis: a critical study. | arachis duranensis is a diploid wild relative of the tetraploid cultivated peanut arachis hypogaea. the literature indicates two 2c genomic dna mean values (genome size) for a. duranensis, 4.92 and 5.64 pg, and intraspecific variation of up to 11% negatively correlated with altitude above sea level of the collection sites has been reported. our recent investigations of arachis species have shown that unrecognized technical problems with peanut material may have influenced previous genome-size da ... | 2001 | 11681606 |
| high relative humidity increases yield, harvest index, flowering, and gynophore growth of hydroponically grown peanut plants. | growth chamber experiments were conducted to study the physiological and growth response of peanut (arachis hypogaea l.) to 50% and 85% relative humidity (rh). the objective was to determine the effects of rh on pod and seed yield, harvest index, and flowering of peanut grown by the nutrient film technique (nft). 'georgia red' peanut plants (14 days old) were planted into growth channels (0.15 x 0.15 x 1.2 m). plants were spaced 25 cm apart with 15 cm between channels. a modified half-hoagland s ... | 2000 | 11725789 |
| growth, pod, and seed yield, and gas exchange of hydroponically grown peanut in response to co2 enrichment. | the effects of elevated co2 on growth, pod, and seed yield, and gas exchange of 'georgia red' peanut (arachis hypogaea l.) were evaluated under controlled environmental conditions. plants were exposed to concentrations of 400 (ambient), 800, and 1200 micromoles mol-1 co2 in reach-in growth chambers. foliage fresh and dry weights increased with increased co2 up to 800 micromoles mol-1, but declined at 1200 micromoles mol-1. the number and the fresh and dry weights of pods also increased with incr ... | 2000 | 11725790 |
| identification of a cdna clone encoding for a galactose-binding lectin from peanut (arachis hypogaea) seedling roots. | a cdna clone obtained from developing peanut (arachis hypogaea) seedling roots, when expressed in escherichia coli and insect cells (sf9) gave a 29 kda subunit protein. the native recombinant protein agglutinates neuraminidase treated human erythrocytes and the agglutination is inhibited by galactose. nucleotide sequence and predicted amino acid sequence analyses indicate that it is different from peanut seed (pna and sgl) and nodule (ngla and nglb) galactose-binding lectins. | 2001 | 11731092 |
| synthesis and protein binding properties of t-antigen containing glycopamam dendrimers. | allyl o-(beta-d-galactopyranosyl)-(1-3)-2-acetamido-2-deoxy-alpha-d-galactopyranoside (8) was prepared in excellent yield from the corresponding galactosyl bromide (6, 7) and allyl 2-acetamido-4,6-benzylidene-2-deoxy-alpha-d-galactopyranoside (5) using hg(cn)2 as a promoter. compound 5 was obtained from n-acetylglucosamine 1 following sequential protecting group strategy and c-4 epimerization as a key step. carboxylic acid functionalized t-antigen derivative 15, obtained by radical addition of 3 ... | 2002 | 11738602 |
| dry matter production and rate of change of harvest index at high temperature in peanut. | the concept of a linear increase in harvest index, dhi/dt, has proven very useful for crop simulation modeling. the effect of high temperature on the response of dhi/dt of pods and seeds of peanut (arachis hypogaea l.) has not been described. the objectives of this work were to determine (i) whether dhi/dt was linear at high temperature, (ii) whether high temperature affected dhi/dt and/or the timing of the linear phase of increase in hi, and (iii) whether there was genotypic variation in the re ... | 2002 | 11756265 |
| factors enhancing agrobacterium tumefaciens-mediated gene transfer in peanut (arachis hypogaea l.). | parameters enhancing agrobacterium-mediated transfer of foreign genes to peanut (arachis hypogaea l.) cells were investigated. an intron-containing beta-glucuronidase uida (gusa) gene under the transcriptional control of camv 35s promoter served as a reporter. transformation frequency was evaluated by scoring the number of sectors expressing gus activity on leaf and epicotyl explants. the 'valencia select' market type cv. new mexico was more amenable to agrobacterium transformation than the 'run ... | 1998 | 11760772 |
| inheritance of a lutescent-leaf color trait in peanut. | a lutescent-leaf color mutant was recently found in the cultivated peanut (arachis hypogaea l.). crosses involving the lutescent-leaf mutant were made both between and within subspecies of hypogaea and fastigiata to determine its inheritance. the f1, f2, and f3 data indicated that two duplicate recessive genes, designated lut1 and lut2, control the lutescent-leaf color trait. no maternal or cytoplasmic effects were detected among progenies from reciprocal hybridization. | 2015 | 11773253 |
| investigation of lotic microbial aggregates by a combined technique of fluorescent in situ hybridization and lectin-binding-analysis. | a technique combining fluorescent in situ hybridization and lectin-binding-analysis (fish-lba) was developed and applied for the simultaneous detection of cellular components and glycoconjugates in lotic microbial aggregates (river snow). river snow aggregates were directly collected from the bulk water phase into coverslip chambers, in which the complete procedure including fixation, fluorescent in situ hybridization, lectin-binding and optical analysis by confocal laser scanning microscopy was ... | 2002 | 11777585 |
| characterization of trophoblast cell populations by lectin histochemistry in canine placenta during development. | the aim of this study was to identify and characterize populations of trophoblast cells in canine placenta during different stages of fetal development using lectin histochemistry. dogs have endotheliochorial placentation and trophoblast cell invasion continues after chorioallantois villous penetration early in pregnancy, leading to formation of a labyrinth. specialized subpopulations of cells differentiate, such as syncytial trophoblast that invades the maternal epithelium early in placentation ... | 2001 | 11787150 |
| effect of dietary supplementation of fatty acids and vitamins on the breeding performance of the carp catla catla. | five isonitrogenous diets (approximately 33% crude protein) were fed to the brood female carp, catla catla (weighing 3.0 to 5.5 kg), for a period of 93 days in order to observe their breeding performance in earthen ponds. diet-i (control) contained only basic ingredients like rice bran, ground-nut oil cake, roasted soybean meal, fish meal and mineral mixture; diet-ii contained added vitamins; diet-iii contained added vitamins and vegetable oil (rich in n-6 polyunsaturated fatty acids, pufa); die ... | 2004 | 11789892 |
| biocompatibility of sweetpotato and peanut in a hydroponic system. | 'georgia red' peanut (arachis hypogaea l.) and tu-82-155 sweetpotato [ipomoea batatas (l.) lam] were grown in monocultured or intercropped recirculating hydroponic systems in a greenhouse using the nutrient film technique (nft). the objective was to determine whether growth and subsequent yield would be affected by intercropping. treatments were sweetpotato monoculture (sp), peanut monoculture (pn), and sweetpotato and peanut grown in separate nft channels but sharing a common nutrient solution ... | 1998 | 11795324 |
| peanut and amaranthus leucocarpus lectins discriminate between memory and naive/quiescent porcine lymphocytes. | lectins are relevant tools to isolate and characterize different cellular sub-populations. in this work, we used the lectins arachis hypogaea (peanut agglutinin, pna) and amaranthus leucocarpus (all), specific for galss1, 3galnac, to characterize naive and memory lymphocytes from pigs, experimentally infected with the porcine rubulavirus (rvp). our results showed that both lectins recognized preferentially lymphocytes with the cd4(+)cd8(+) phenotype (p<0.05). the phenotypic analysis of the cells ... | 2002 | 11825599 |
| influence of year and planting date on fatty acid chemistry of high oleic acid and normal peanut genotypes. | the effect of an early-, mid-, or late-season planting date on the fatty acid chemistry of four high oleic acid, one mid oleic acid, and five normal oleic acid peanut (arachis hypogaea l.) genotypes was evaluated over a three year period. oleic acid was also compared to other fatty acids and to indices of oil quality. high-oleic genotypes included sunoleic 97r, uf98326, uf99621, and 88x1b-olbc1-6-1-3-1-b2-b with a mean oleic acid content between 77.8 and 82.5%. florida mdr98, a mid-oleic cultiva ... | 2002 | 11853521 |
| dendritic galactosides based on a beta-cyclodextrin core for the construction of site-specific molecular delivery systems: synthesis and molecular recognition studies. | in order to evaluate the ability of multivalent glycosides based on a beta-cyclodextrin core as site-specific molecular carriers, a study on both the inclusion complexation behaviour and lectin binding affinity of branched and hyperbranched beta-cyclodextrins is presented. a series of cluster galactosides constructed on beta-cyclodextrin scaffolds containing seven 1-thio-beta-lactose or beta-lactosylamine bound to the macrocyclic core through different spacer arms were synthesised. in addition, ... | 2002 | 11857696 |
| in vivo volatile emissions from peanut plants induced by simultaneous fungal infection and insect damage. | peanut plants, arachis hypogaea, infected with white mold. sclerotium rolfsii, emit a blend of organic compounds that differs both quantitatively and qualitatively from the blend emitted from plants damaged by beet armyworm (baw; spodoptera exigua) larvae or from uninfected, undamaged plants. attackby baw induced release of lipoxygenase products (hexenols, hexenals, and hexenyl esters), terpenoids, and indole. the plant-derived compound methyl salicylate and the fungal-derived compound 3-octanon ... | 2002 | 11868672 |
| impact of vitamin a supplementation through different dosages of red palm oil and retinol palmitate on preschool children. | red palm oil (5 ml and 10 ml), ground nut oil fortified with 400 and 800 retinol equivalent retinol palmitate, and ground nut oil (5 and 10 ml), were administered to six groups of preschool children (four experimental and two control groups) in randomly assigned balwadis of ramanathapuram district of tamil nadu for a period of 7 months, to monitor the difference in the efficacy of the mode of supplementation and the optimum dose for improving vitamin a status. results show that red palm oil grou ... | 2002 | 11871368 |
| characterization of a pinus pinaster cdna encoding an auxin up-regulated putative peroxidase in roots. | as part of a study to identify host plant genes regulated by fungal auxin during ectomycorrhiza formation, we differentially screened a cdna library constructed from roots of auxin-treated pinus pinaster (ait.) sol. seedlings. we identified three cdnas up-regulated by auxin. sequence analysis of one of these cdnas, ppprx75, revealed the presence of an open reading frame of 216 amino acids with the characteristic consensus sequences of plant peroxidases. the deduced amino acid sequence showed hom ... | 2002 | 11874719 |
| peanut roots as a source of resveratrol. | a potent antioxidant, resveratrol (3,4',5-trihydroxystilbene), was extracted using 80% methanol from peanut roots (arachis hypogaea l.), isolated with a solid-phase extraction column, purified by a semipreparative hplc, and identified with 1h nmr and ms. the highest and lowest resveratrol contents in the peanut roots of 2000 fall and 2001 spring crops were 1.330 and 0.130 mg/g and 0.063 and 0.015 mg/g, respectively. when the dehydrated peanut root powders of spring and fall crops were combined a ... | 2002 | 11879054 |
| isolation of lysophosphatidic acid phosphatase from developing peanut cotyledons. | the soluble fraction of immature peanut (arachis hypogaea) was capable of dephosphorylating [(3)h]lysophosphatidic acid (lpa) to generate monoacylglycerol (mag). the enzyme responsible for the generation of mag, lpa phosphatase, has been identified in plants and purified by successive chromatography separations on octyl-sepharose, blue sepharose, superdex-75, and heparin-agarose to apparent homogeneity from developing peanuts. this enzyme was purified 5,048-fold to a final specific activity of 8 ... | 2002 | 11891254 |
| carbohydrate expression profile of colorectal cancer cells is relevant to metastatic pattern and prognosis. | carbohydrate expression of cancer cells is closely related to the metastatic nature of colorectal cancer. in the present study we investigated the relevance of carbohydrate expression profiles of colorectal cancer cells in the primary lesion to metastatic distribution patterns as well as prognosis in 134 cases. carbohydrate expression was estimated by histochemistry with 17 kinds of lectins and 3 kinds of lewis-related monoclonal antibodies (mabs), and correlations between the staining and clini ... | 2002 | 11918084 |
| linkage between loci controlling nodulation and testa variegation in peanut. | linkage of loci controlling nodulation (n(1)) and testa variegation (v) was studied for cultivated peanut (arachis hypogaea l.). the lines m4-2 (nonnodulating, variegated; vvn(1)n(1)n(2)n(2)n(3)n(3)) and uf 487a (nodulating, nonvariegated; vvn(1)n(1)n(2)n(2)n(3)n(3)) were used as parents in the crosses m4-2 x uf 487a, m4-2 x (uf 487a x m4-2), and their reciprocals. individual plants were evaluated for nodulation and testa variegation in the f(1), f(2), f(3), f(1)bc(1), and f(2)bc(1) generations. ... | 2015 | 11948220 |
| the activity for the induction of the sperm acrosome reaction localises in the outer layers and exists in the high-molecular-weight components of the egg-jelly of the newt, cynops pyrrhogaster. | localisation of the acrosome reaction inducing activity in egg-jelly was examined in the newt, cynops pyrrhogaster. the jelly has six layers: the j0, j1, j2, j3, j4 and st layers. jelly was mechanically dissected and placed on a millipore filter. when sperm were added from the outer surface side of the jelly, most of them exhibited the acrosome reaction after passing through the jelly. when egg-jelly was divided into four layers, strong activity for the induction of acrosome reaction was detecte ... | 2002 | 11964085 |
| salt- and glyphosate-induced increase in glyoxalase i activity in cell lines of groundnut (arachis hypogaea). | glyoxalase i (ec 4.4.1.5) activity has long been associated with rapid cell proliferation, but experimental evidence is forthcoming, linking its role to stress tolerance as well. proliferative callus cultures of groundnut (arachis hypogaea l. cv. jl24) showed a 3.3-fold increase in glyoxalase i activity during the logarithmic growth phase, correlating well with the data on fw gain and mitotic index. inhibition of cell division decreased glyoxalase i activity and vice versa, thus further corrobor ... | 2002 | 11975722 |
| a quantitative evaluation of radiolabelled lectin retention on oral mucosa in vitro and in vivo. | previous work has identified lectins that bind to the cells present on the oral mucosa for their potential use as a means of retaining a drug delivery system on the mucosal surfaces of the mouth. in this study, a radiolabelling technique was developed to allow the quantification of lectin binding to human buccal cells in vitro, and the retention of the lectins in the oral cavity of a rat model in vivo. lectins were labelled with 99mtc using a cyclic diethylene triamine pentaacetic acid conjugati ... | 2002 | 11976016 |
| trypanosoma cruzi i and trypanosoma cruzi ii: recognition of sugar structures by arachis hypogaea (peanut agglutinin) lectin. | epimastigote culture forms of different isolates of trypanosoma cruzi from different mammal hosts, humans, and vectors were tested with fitc-conjugated peanut agglutinin lectin (pna-fitc). the parasites maintained in axenic medium, liver infusion tryptose. were evaluated by flow cytometric analyses; whereas t. cruzi i (tcl), which is associated with the sylvatic transmission cycle, was labeled in high percentages with pna (88-99.2%), t. cruzi ii (tcii) (parasites associated with domiciliar cycle ... | 2002 | 12099431 |
| evaluation of the acrosome reaction and viability in buffalo spermatozoa using two staining methods: the effects of heparin and calcium ionophore a23187. | in this study, we investigated the effect of heparin and calcium ionophore a23187 on in vitro induction of buffalo sperm acrosome reaction (ar). two methods for detection of the ar and viability were employed. fluorescein isothiocyanate-conjugated arachis hypogea agglutinin (fitc-pna) was used as a vital stain in combination with ethidium homodimer-1 (ethd-1) to determine the acrosome status of viable spermatozoa. in another experiment, trypan blue replaced ethd-1 to differentiate live and dead ... | 2002 | 12121571 |
| ruptured achilles tendons show increased lectin stainability. | to ascertain whether lectins could be a useful tool for investigation of the extracellular matrix of degenerated and normal tendons. | 2002 | 12131241 |
| selection of alternative genetic sources of large-seed size in virginia-type peanut: evaluation of sensory, composition, and agronomic characteristics. | jenkins jumbo, the ancestral source of large-seed size in the virginia market type (arachis hypogaea l.), has been shown to have a deleterious effect on flavor of peanut. the pervasiveness of jenkins jumbo in the ancestry of large-seeded germplasm contributes to the generally less intense roasted peanut flavor of u.s. cultivars of the virginia market type. as a remedy to this problem, alternative sources of large-seed size were sought. nine large-seeded selections, with nc 7 and florunner as che ... | 2002 | 12166976 |
| allelopathic potential and chemical constituents of volatiles from ageratum conyzoides under stress. | the allelopathic potential of ageratum conyzoides was investigated under different environmental stress conditions, including nutrient deficiency. physical damage, 2.4-d treatment, competition with bidens pilosa, infection with erysiphe cichoracearum, and feeding by aphiids gossypii. the inhibitory effects of a. conyzoides volatiles on peanut (arachis hypogaea), redroot amaranth (amaranthus retroflexus), cucumber (cucumis sativus), and ryegrass (lolium multiforum) increased when plants were grow ... | 2002 | 12184395 |
| developmentally regulated dual-specificity kinase from peanut that is induced by abiotic stresses. | tyrosine (tyr) phosphorylation represents an important biochemical mechanism to regulate many cellular processes. no tyr kinase has been cloned so far in plants. dual-specificity kinases are reported in plants and the function of these kinases remains unknown. a 1.7-kb cdna that encodes serine/threonine/tyr (sty) kinase was isolated by screening peanut (arachis hypogaea) expression library using the anti-phospho-tyr antibody. the histidine-tagged recombinant kinase histidine-6-sty predominantly ... | 2002 | 12226517 |
| lipids, proteins, and structure of seed oil bodies from diverse species. | oil bodies isolated from the mature seeds of rape (brassica napus l.), mustard (brassica juncea l.), cotton (gossypium hirsutum l.), flax (linus usitatis simum), maize (zea mays l.), peanut (arachis hypogaea l.), and sesame (sesamum indicum l.) had average diameters that were different but within a narrow range (0.6-2.0 [mu]m), as measured from electron micrographs of serial sections. their contents of triacylglycerols (tag), phospholipids, and proteins (oleosins) were correlated with their size ... | 1993 | 12231682 |
| a peanut nodule lectin in infected cells and in vacuoles and the extracellular matrix of nodule parenchyma. | root nodules on peanut (arachis hypogaea l.) accumulate a galactose/lactose-binding lectin that is similar, but not identical, to the major seed lectin in peanut. the function of the peanut nodule lectin (pnl) is not known. in the current study, we have investigated the location of lectin in the nodule using immunogold labeling and enzyme-linked immunosorbant assays (elisa). lectin was most abundant in the nodule parenchyma, where it accumulated in vacuoles, suggesting a possible role as a veget ... | 1994 | 12232084 |
| [how to increase food production?]. | pressure of population on cultivable land, serious soil erosion, and low productivity due to scarcity of inputs have hampered efforts to provide an adequate diet for the population of rwanda. until the present, production has increased about as rapidly as population, but rwanda is not totally self-sufficient in food, future climatic conditions may be less favorable than those of the past, technical and resource constraints are likely to increase, and little new land will be available for cultiva ... | 1984 | 12267714 |
| [reasons for senegalese migration determined by ethnic background and social status]. | recent studies of female labor force participation and migration in sub-saharan africa have disproven earlier beliefs about the passive role of women in migration. monoculture of ground nuts throughout senegal has let to a monetarization of the traditional subsistence economy, disturbing the already fragile equilibrium of rural life. neglect of subsistence crops has meant that the rural population must depend heavily on imported rice. women, who bear the main responsibility for feeding their fam ... | 1991 | 12343342 |
| enzymatic formation of an unnatural c(6)-c(5) aromatic polyketide by plant type iii polyketide synthases. | [reaction: see text] substrate specificities of plant polyketide synthases (pkss) were investigated using analogues of malonyl-coa, the extension unit of the polyketide chain elongation reactions. when incubated with methylmalonyl-coa and 4-coumaroyl-coa, plant pkss (chalcone synthase from scutellaria baicalensis, stilbene synthase from arachis hypogaea, and benzalacetone synthase from rheum palmatum) afforded an unnatural c(6)-c(5) aromatic polyketide, 1-(4-hydroxyphenyl)pent-1-en-3-one, formed ... | 2002 | 12375903 |
| hypoglycemic and hypolipidemic effects of aqueous extract of arachis hypogaea in normal and alloxan-induced diabetic rats. | the hypoglycemic and hypolipidemic effect of aqueous extract of arachis hypogaea was investigated in normal and alloxan-induced diabetic rats. the extract caused a significant (p < 0.05) decrease of fasting blood glucose of both normal and alloxan-induced diabetic rats from 102.60 +/- 1.65 mg/dl to 88.79 +/- 0.94 mg/dl for normal and 189.0 +/- 30.79 mg/dl to 107.55 +/- 1.54 mg/dl for alloxan-induced diabetic rats. the extract also caused a significant (p < 0.05) decrease in serum triglyceride, t ... | 2002 | 12403165 |
| rbc t activation and hemolysis in a neonatal intensive care population: implications for transfusion practice. | reports of transfusion-associated hemolysis in infants with t-activated rbcs have led to the suggestion that infants should be screened and provided with low-titer anti-t blood components. t-activated rbcs react with the lectins arachis hypogea and glycine soja; variants of t (th and tx) and tk also react with a. hypogea, but not g. soja. although tk is not a true variant of t, for the purposes of this study, all rbcs that are reactive with a. hypogea but are not reactive with g. soja are called ... | 2002 | 12421215 |