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membranes markers in highly purified clathrin-coated vesicles from cucurbita hypocotyls.the two plasma-membrane (pm) markers: 1,3-β-glucan synthase and naphthylphthalamic-acid-binding capacity are localized in two peaks of activity on isopycnic ficoll/d2o gradients prepared from a zucchini (cucurbita pepo l.) hypocotyl post-microsomal fraction. the denser peak overlaps with the major distribution of clathrin and represents a region of the gradient enriched in coated vesicles (cv). further purification of the pooled cv-fractions has shown that these pm marker activities are not born ...199124193754
calcium deficiency and auxin transport in cucurbita pepo l. seedlings.hypocotyls of cucurbita pepo l. (zucchini) seedlings grown at low calcium levels (0.1 mm) showed reduced basipetal polar transport of the auxins indole-3-acetic acid and 1-naphthylacetic acid in comparison with control plants grown at 5 mm ca(2+). the contribution to overall transmembrane auxin transport of the symport uptake carrier was unchanged by calcium deficiency, but the efflux carrier of the auxin-anion uniport was less active and the sensitivity of auxin transport to n-1-naphthylphthala ...199124193855
symplastic continuity between mesophyll and companion cells in minor veins of mature cucurbita pepo l. leaves.dye-coupling studies have been undertaken to determine whether plasmodesmata between intermediary cells (companion cells) and bundle-sheath cells in the minor veins of mature cucurbita pepo l. leaves are open to passage of low-molecular-weight compounds. the abaxial phloem of these veins was exposed by stripping the lower epidermis of the leaf and removing the spongy-mesophyll cells by abrasion. lucifer yellow, or 6-carboxyfluorescein, were microinjected into intermediary cells by iontophoresis, ...198924201418
viability of cururbita pepo pollen: biophysical and structural data.during ageing of the short-lived pollen grains of cucurbita pepo l., water loss was examined in relation to viability using biophysical ((1)h-nuclear magnetic resonance, nmr) and cytological methods (fluorochromatic reaction test, freezefracture and scanning electron microscopy). a semi-logarithmic representation of the pollen weight loss demonstrated the complexity of the dehydration process. a the study of proton loss using (1)h-nmr indicated that two major releases water of had taken place, e ...198924201515
localization of glyoxylate-cycle marker enzymes in peroxisomes of senescent leaves and green cotyledons.crude particulate homogenates from leaves of barley (hordeum vulgare l.), rice (oryza sativa l.), leaf-beet (beta vulgaris var. cicla l.) and pumpkin (cucurbita pepo l.) cotyledons were separated on sucrose density gradients. the peroxisomal fractions appeared at a buoyant density of 1.25 g·cm(-3) and contained most of the activities of catalase (ec 1.11.1.6), and hydroxypyruvate reductase (ec 1.1.1.81) on the gradients. in peroxisomal fractions from detached leaves and green cotyledons incubate ...199024202025
heterogeneity of auxin-accumulating membrane vesicles from cucurbita and zea: a possible reflection of cell polarity.when microsomes from hypocotyls of cucurbita pepo l. or coleoptiles of zea mays l. were centrifuged on dextran-sucrose gradients a heterogeneity of auxin-accumulating vesicles was observed. vesicles from the top part of the gradient showed saturable, specific accumulation of indole-3-acetic acid with only a small stimulation by phytotropins, and with very few binding sites for 1-n-naphthylphthalamic acid. in the vesicles from the lower part of the gradient, net accumulation of indole-3-acetic ac ...198924212422
somatic embryogenesis and plant regeneration of squash cucurbita pepo l cv. yc 60.plant regeneration from tissue cultures of summer squash, cucurbita pepo l., cv. yc60, has been observed. somatic embryos organized from shoot apex derived callus cultured on murashige and skoog (ms) medium supplemented with 1.2 mg/l 2,4,5-trichlorophenoxyacetic acid, 0.8 mg/l benzylaminopurine, and 0.1 mg/l kinetin. embryos developed into plantlets by transfer of immature somatic embryos to ms medium with 0.05 mg/l naa and 0.05 mg/l kinetin. regenerated plants appeared morphologically normal an ...199124213662
plant development in long-term embryogenic callus lines of cucurbita pepo.embryogenic callus derived from pumpkin hypocotyl segments was induced and maintained for 15 years on ms medium supplemented with the auxins iba (4.9 μm), 2, 4-d (4.5 μm) or iaa (5.7 μm). on induction media continued embryo maturation and development of adult plants typically failed. therefore, small embryogenic clumps and individually isolated embryos were subcultured two to four times on one of the conversion media: ms supplemented with 1.5% sucrose and (a) no hormone, (b) 2.9 μm iaa, (c) 5.7 ...199124213663
import of peroxisomal hydroxypyruvate reductase into glyoxysomes.a new procedure was used to purify the peroxisomal matrix enzyme hydroxypyruvate reductase (hpr) from green leaves of pumpkin (cucurbita pepo l.) and spinach (spinacia oleracea l.). monospecific antibodies were prepared against this enzyme in rabbits. immunoprecipitation of hpr from watermelon (citrullus vulgaris schrad.) yielded a single protein with a subunit molecular weight of 45 kda. immunohistochemical labeling of hpr was found exclusively in watermelon microbodies. isolated polyadenylated ...198824220768
bisulfite interacts with binding sites of the auxin-transport inhibitor n-1-naphthylphthalamic acid.the affinity of the auxin-transport inhibitor n-1-naphthylphthalamic acid (npa) for membrane particles as well as for solubilized binding sites from cucurbita pepo l. hypocotyls was reduced by low concentrations of bisulfite (half-maximal inhibition at 2·10(-3)-3·10(-3) m). two membrane fractions obtained by sedimentation aided with polyethylene glycol showed differential sensitivity to bisulfite. other oxidizing or reducing substances tested at 1 mm had no effect, except for n-ethylmaleimide (8 ...198824220862
a new set of regulatory molecules in plants: a plant phospholipid similar to platelet-activating factor stimulates protein kinase and proton-translocating atpase in membrane vesicles.1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine, an ether phospholipid from mammals known as platelet-activating factor (paf), specifically stimulates proton transport in zucchini (cucurbita pepo l.) microsomes (g.f.e. scherer, 1985, biochem. biophys. res. commm. 133, 1160-1167). when plant lipids were analyzed by two-dimensional thin-layer chromatography a lipid was found with chromatographic properties very similar to the paf (g.f.e. scherer and b. stoffel, 1987, planta, 172, 127-130). this lip ...198824221719
auxin carriers in cucurbita vesicles : i. imposed perturbations of transmembrane ph and electrical potential gradients characterised by radioactive probes.the behaviour of radioactive probes of transmembrane ph gradients ([(14)c]butyric acid) and electrical potential gradients ((86)rb(+) and s(14)cn(-)) has been characterised in membrane vesicles from cucurbita pepo l. hypocotyls subjected to changing gradients and ionophore-mediated changes in the conductivity of k(+) and h(+) ions. the vesicles are sealed in the presence of 25 mm kcl. the k(+) ionophore valinomycin changes the distribution of (86)rb(+) and of s(14)cn(-) in opposite directions, w ...198724225712
auxin carriers in cucurbita vesicles : ii. evidence that carrier-mediated routes of both indole-3-acetic acid influx and efflux are electroimpelled.the association at 0° c of [(3)h]indole-3-acetic acid (iaa) with membrane vesicles prepared from cucurbita pepo l. hypocotyls at ph 7.9 and resuspended at ph 6.0 was greatly diminished by osmotic shrinkage. nonradioactive iaa inhibited a large proportion of this association thus revealing a saturable component, also decreased by osmotic shrinkage, which mainly represents operation of an auxin uptake carrier, with saturable binding having only a minor role. the contribution of this carrier to the ...198724225713
auxin carriers in cucurbita vesicles : iii. specificity, with particular reference to 1-naphthylacetic acid.carrier-mediated uptake of indole-3-acetic acid (iaa) by microsomal vesicles from cucurbita pepo l. hypocotyls was strongly inhibited by 2,4-dichlorophenoxyacetic acid (2,4-d; i 50= 0.3 μm) but only weakly by 1-naphthylacetic acid (naa). the fully ionised auxin indol-3-yl methanesulphonic acid also inhibited (i 50=3 μm). the same affinity ranking of these auxins for the uptake carrier, an electroimpelled auxin anion-h(+) symport, is demonstrable in hypocotyl segments. the specificity of the auxi ...198724225714
a plant lipid and the platelet-activating factor stimulate atp-dependent h(+) transport in isolated plant membrane vesicles.a plant lipid was isolated from zucchini (cucurbita pepo l.) membranes and from soybean (glycine max [l.] merr) phospholipids by thinlayer chromatography and further purified by high-performance liquid chromatography. this plant lipid was chromatographically very similar to the platelet-activating factor, an ether phospho-lipid with hormone-like properties found in mammals. both the plant lipid and the platelet-activating factor stimulated atp-dependent h(+) transport in isolated membrane vesicl ...198724225797
an endogenous rna-synthesis-promoting oligopeptide from cucurbita pepto var. patissonina.the effect of cppti (cucurbita pepo patissonina trypsin inhibitor) on the growth of cucurbita pepo var patissonina (white bush) was examined. plants treated with cppti grew faster than untreated plants and similarly to those treated with gibberellic acid. isolated cell nuclei from plants treated with cppti showed that of the three dna-dependent rna polymerases assayed, rna polymerase i (ec2.7.7.6) activity was preferentially elevated.198824226178
quality of a stress-sensitive cucurbita pepo l. pollen.the quality of cucurbita pepo l. pollen was studied using field pollinations and the fluorochromatic-reaction test. the extreme sensitivity of this pollen to dehydration and ageing is demonstrated. controlled stress applied to mature pollen leads to the development of seedless fruits. molecular signals seem to be involved in the induction of this parthenocarpy. these results indicate the existence of distinct sequences involved in the completion of the fertilization program of pollen. with polle ...198724227273
evidence supporting a model of voltage-dependent uptake of auxin into cucurbita vesicles.the accumulation of [(14)c]indole-3-acetic acid (iaa), of [(3)h]tetra-phenyl phosphonium ion as a membrane potential probe, and of [(14)c]butyric acid as probe for ph gradients was studied with membrane vesicles from etiolated hypocotyls of cucurbita pepo. ion gradients (k(+), h(+)) were applied in the presence and absence of specific ionophores e.g. valinomycin or carbonylcyanide m-chlorophenylhydrazone. in all cases tested, the accumulation of [(14)c]iaa equals neither potential probe nor ph-p ...198624232555
effect of cytokinins on ribosomal rna gene expression in excised cotyledons of cucurbita pepo l.excised pumpkin (cucurbita pepo l.) cotyledons were used to investigate the effects of two different types of cytokinins: n(6)-benzyladenine and n1-(2-chloro-4-pyridyl)-n2-phenylurea on rna synthesis in isolated nuclei. treatment of cotyledons with both cytokinins resulted in a rapid enhancement of nuclear rna-polymerase-i activity (ec 2.7.7.6). maximum stimulation of rna polymerase i, responsible for rrna synthesis, was observed 4-6 h after the start of cytokinin action. the activity of rna pol ...198724232967
pollen gene expression analysed by micro-isoelectric focusing of proteins from isolated pollen grains in cucurbita pepo l.isoelectric focusing of proteins from single pollen grains of cucurbita pepo l. has been developed for large scale study of pollen grain populations' heterogeneity. forty to forty-five protein bands from one pollen grain are revealed after silver staining. applications of this technique to pollen grain populations from different genotypes are described in this paper. possible applications and limits of this technique are discussed with respect to plant breeding especially for the measure of gene ...198624240746
different at-rich satellite dnas in cucurbita pepo and cucurbita maxima.the at-rich highly repeated satellite dna of cucurbita pepo (zucchini) and cucurbita maxima (pumpkin) were cloned and their dna structure was investigated. dna sequencing revealed that the repeat length of satellite dna in cucurbita pepo is 349-352 base pairs. the percentage of at-base pairs is about 61%. this satellite is highly conserved in restriction enzyme pattern and dna sequence; sequence heterogeneity is about 10%. in contrast, the satellite dna of cucurbita maxima has a repeat length of ...198624240758
genetics of resistance to red pumpkin beetle (a ulacophora foveicollis) in summer squash (cucurbita pepo l.).resistance to red pumpkin beetle in summer squash was found to be controlled by polygenes. diallel and triple test cross analysis revealed the preponderance of non-additive and additive gene effects for resistance respectively. absence of epistasis for resistance was indicated by both tests.198724241196
calcium-binding and its effect on circular dichroism of plant calmodulin.the ca(2+)-binding properties of calmodulin purified from zucchini (cucurbita pepo l.) has been determined. a value of 3.3 mol ca(2+) per mol of zucchini calmodulin was measured at ph 7.5 by equilibrium chromatography. the far-and near-uv circular-dichroic spectra of the ca(2+)-and mg(2+)-saturated as well as from the metal-free forms of zucchini calmodulin reveal that upon ca(2+)-binding the α-helix content increases. a comparison with the spectra of vertebrate calmodulin indicates that both ca ...198524241435
plant virus-induced changes in aphid population development and temporal fluctuations in plant nutrients.cucurbita pepo plants were infected with zucchini yellow mosaic virus or maintained noninfected.aphis gossypii, which transmits the virus, lived longer and produced more offspring on infected than on noninfected plants. on infected plants, the intrinsic rate of natural increase fora. gossypii increased with time after inoculation. in a similar experiment, concentrations of phloem sap nutrients, including free amino acids, total protein, and sugars from infected and noninfected plants were compar ...199424242121
squash leaf glandular trichome volatiles: identification and influence on behavior of female pickleworm moth [diaphania nitidalis (stoll.)] (lepidoptera: pyralidae).fourteen volatile compounds occurring in leaf trichomes of yellow squash (cucurbita pepo l. cv. early prolific straightneck) were identified. these compounds accounted for 83.5% of the volatile matrix. ubiquitous constituents of the epidermis (myristic, palmitic, and stearic acids,n-tricosane, andn-pentacosane) accounted for 73.7%; these compounds were not bioassayed. the volatileso-,m-, andp-xylene, toluene, 2-heptanone, (r)-(+)- and (s)-(-)-limonene, and germacrene d were tested for their infl ...199424242732
genetic control of plastid carotenoids and transformation in the skin of cucurbita pepo l. fruit.the influence of allelic state of gene b on skin pigmentation in two cultivars of cucurbita pepo l. has been studied. total carotenoids were lower at early stages of fruit development in cultivar (cv.) 'early prolific' (ep) bb yy fruit skin, than in ep b (+) b (+) yy fruit skin, but no differences were observed in total skin carotenoids twenty days after anthesis. total carotenoids were lower in cv. 'fordhook zucchini' (fz) bb yy fruit skin, than in fz b (+) b (+) yy fruit skin at all developmen ...198424257820
squash leaf curl virus (slcv): a serious disease threatening cucurbits production in palestine.the incidence of squash leaf curl disease and molecular characterization of the palestinian isolate of squash leaf curl virus [slcv-(pal)] are described in this study. symptomatic leaf samples obtained from squash (cucurbita pepo), watermelon [citrullus lanatus (thunb.)], and cucumber (cucumis sativus l.) plants were tested for slcv-[pal] infection by pcr and rca. slcv was also found to occur naturally in chenopodium murale, convolvulus sp, and prosporis farcta which showed yellowing. the diseas ...201424258392
native phytochrome: immunoblot analysis of relative molecular mass and in-vitro proteolytic degradation for several plant species.the relative molecular mass (mr) of the native phytochrome monomer from etiolated cucurbita pepo l., pisum sativum l., secale cereale l. and zea mays l. seedlings has been determined using immunoblotting to visualize the chromoprotein in crude extracts subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a single phytochrome band is observed for each plant species when the molecule is extracted under conditions previously demonstrated to inhibit the proteolysis of native avena ...198424258779
characteristics of organic acids in the fruit of different pumpkin species.the aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. the amount of acids immediately after fruit harvest and after 3 months of storage was compared. the content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (hplc). three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varie ...201424262577
auxin transport in membrane vesicles from cucurbita pepo l.association of (14)c-labelled indole-3-acetic acid (iaa) with membrane particles from zucchini (cucurbita pepo l.) hypocotyls - previously described as "site iii binding" (m. jacobs and r. hertel, 1978, planta 142, 1-10) - is reinterpreted as a carrier-mediated uptake into closed and sealed vesicles driven by a ph gradient. accumulation of the radioactive auxin is saturable, sensitive to the protonophore, carbonyl cyanide p-trifluoromethoxyphenyl hydrazone (fccp), and to nigericin, and requires ...198324264147
protein phosphorylation and its regulation by calcium and calmodulin in membrane fractions from zucchini hypocotyls.protein-kinase activity has been found to be associated with a membrane fraction obtained from dark-grown zucchini (cucurbita pepo l., cv. senator) hypocotyl hooks. proteins of this membrane fraction were used as protein substrates. the effects of mg(2+), na(+) and k(+) on phosphorylation, measured as (32)p incorporation, was investigated. the kinetics of phosphorylation of the individual protein peptides indicate the presence of specific phosphatase activity. phosphorylation activity is strongl ...198324264929
superoxide, hydrogen peroxide, and the gelling of phloem sap from cucurbita pepo.the possible involvement of superoxide and hydrogen peroxide in the oxidative gelling of phloem exudate from cucurbita pepo. was investigated. neither superoxide dismutase (ec 1.15.1.1) nor catalase (ec 1.11.1.6) inhibited the reaction. although catalase could not be detected in exudate, both peroxidase (ec. 1.11.1.7) and superoxide dismutase were present in reasonable amounts. polyacrylamide gel electrophoresis revealed one major and one minor isozyme of superoxide dismutase, both of which were ...198224276155
cloning of cdna for phytochrome from etiolated cucurbita and coordinate photoregulation of the abundance of two distinct phytochrome transcripts.we have isolated several cdna clones for phytochrome from a dicot, cucurbita pepo l. cv. black beauty (zucchini), and have used them to study the regulation of cucurbita phytochrome mrna levels. a cdna library was constructed from poly(a)(+) rna isolated from etiolated cucurbita hypocotyl hooks and enriched for phytochrome mrna by size fractionation. this library was screened with a (32)p-labeled fragment isolated from an avena phytochrome cdna clone. several putative phytochrome clones were iso ...198724301311
characterization of repetitive elements in several cucurbita species.about 1.3×10(5) copies of a tandemly arranged, 351 base pair element constitute from 4 to 8% of the cucurbita pepo genome. a homologous, high copy number repetitive element is present in the genomes of c. moschata and c. foetidissima, but not in c. maxima or cucumis sativus. array lengths of at least 65 members have been detected for c. pepo, and 140 for c. moschata. the nucleotide sequence of six c. pepo repeat units were determined and each was found to be unique, differing from the others at ...198724301312
in search of a reversible stage of photoinhibition in a higher plant: no changes in the amount of functional photosystem ii accompany relaxation of variable fluorescence after exposure of lincomycin-treated cucurbita pepo leaves to high light.pumpkin (cucurbita pepo l.) leaves in which chloroplast protein synthesis was inhibited with lincomycin were exposed to strong photoinhibitory light, and changes in fo, fm, fv/fm and in the amount of functional photosystem ii (o2 evolution induced by saturating single-turnover flashes) were monitored during the high-light exposure and subsequent dark or low-light incubation. in the course of the photoinhibitory illumination, fm, fv/fm and the amount of functional ps ii declined continuously wher ...199524301535
biochemical and physiological mechanisms underlying effects of cucumber mosaic virus on host-plant traits that mediate transmission by aphid vectors.the transmission of insect-vectored diseases entails complex interactions among pathogens, hosts and vectors. chemistry plays a key role in these interactions; yet, little work has addressed the chemical ecology of insect-vectored diseases, especially in plant pathosystems. recently, we documented effects of cucumber mosaic virus (cmv) on the phenotype of its host (cucurbita pepo) that influence plant-aphid interactions and appear conducive to the non-persistent transmission of this virus. cmv r ...201424329574
tocopherol from seeds of cucurbita pepo against diabetes: validation by in vivo experiments supported by computational docking.tocopherol from raw pumpkin seeds has been reported to be effective in the alleviation of diabetes through its antioxidant activities. this study evaluates the antidiabetic activities of the tocopherol fraction of raw seeds of cucurbita pepo l. (cpse) in a diabetic rat model. in addition, the putative action mechanisms of its botanicals were computationally investigated.201324344360
affinity chromatography of a lectin from robinia pseudoacacia l. and demonstration of lectins in sieve-tube sap from other tree species.a carbohydrate-binding protein in the sievetube sap of robinia pseudoacacia l. was isolated by affinity chromatography on d-galactosamine coupled to ch-sepharose 4b. the yield of the purified protein was 41,3 μg/ml of sieve-tube sap or 5,7% of the total protein. in disk electrophoresis at an alkaline and acidic ph the lectin appeared to be homogenous. it has a molecular weight of about 100,000 and consists of four types of subunits (mol wt. 70,000, 60,000, 32,500, 25,000). the complex formation ...197924407326
auxin binding to subcellular fractions from cucurbita hypocotyls: in vitro evidence for an auxin transport carrier.an auxin binding sive, with characteristics different from the previously described auxin binding sites i and ii in maize coleoptiles, is reported in homogenates of zucchini (cucurbita pepo l. cv. black beauty) hypocotyls. evidence from differential centrifugation and sucrose and metrizamide density gradients indicates that the site is localized on the plasma membrane. the site has a kd of 1-2×10(-6) m for indole acetic acid and has a ph optimum of 5.0. binding specificity measured with several ...197824407991
dark fixation of co2 during gluconeogenesis by the cotyledons of cucurbita pepo l.we did this work to discover the pathway of co2 fixation into sugars in the dark during gluconeogenesis by the cotyledons of 5-day-old seedlings of cucurbita pepo l. we paid particular attention to the possibility of a contribution from ribulosebisphosphate carboxylase. the detailed distribution of (14)c after exposure of excised cotyledons to (14)co2 in the dark was determined in a series of pulse and chase experiments. after 4s in (14)co2, 89% of the (14)c fixed was in malate and aspartate. in ...197824414566
in vitro effects of monoterpenes on chloroplast membranes.a chloroplast preparation was extracted from squash (cucurbita pepo (l.) var. senator). enrichment of intact chloroplasts was achieved by continuous free-flow electrophoresis. the addition of monoterpenes, detergent and free fatty acids changed the elecrophoretic separation pattern characteristically. monoterpene-dependent degradation of envelope membranes could be prevented by addition of α-tocopherol prior to monoterpene incubation.photosynthetic electron transport of photosystem ii was comple ...199124414970
gel filtration of particle-bound phytochrome.pelletable phytochrome from hypocotyl hooks of cucurbita pepo l. seedlings has been separated into two fractions by gel filtration on sepharose cl-2b. one fraction with a k av of 0.7 was detected only after red irradiation (in vivo or in vitro). this separated from a ribonucleoprotein fraction during gel filtration. the weak interaction with ribonucleo-protein which required magnesium (optimal at 10 mm) was overcome by high salt concentrations and prevented by ribonuclease treatment. the second ...197724419784
temperature-dependent changes in photosystem ii heterogeneity support a cycle of photosystem ii during photoinhibition.high light treatments were given to attached leaves of pumpkin (cucurbita pepo l.) at room temperature and at 1°c where the diffusion- and enzyme-dependent repair processes of photosystem ii are at a minimum. after treatments, electron transfer activities and fluorescence induction were measured from thylakoids isolated from the treated leaves. when the photoinhibition treatment was given at 1°c, the photosystem ii electron transfer assays that were designed to require electron transfer to the p ...199024420463
the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis.proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ...197624424985
phytochrome and phosphotungstate-chromate-positive vesicles from cucurbita pepo l.the phosphotungstic acid-chromic acid (pta-cro3) stain, putatively specific for the plasma membrane of plants, has been used in an attempt to monitor the distribution of this membrane in a 20,000 x g particulate fraction from cucurbita hypocotyl hooks. on discontinuous sucrose gradients, the relative distributions of the phytochrome and pta-cro3-positive vesicles present in this fraction appear to be correlated. when intact tissue is stained, however, other components, in addition to the plasma ...197724425221
filament formation in vitro of a sieve tube protein from cucurbita maxima and cucurbita pepo.phloem proteins of the sieve tube exudate from cucurbita maxima duch. and cucurbita pepo l. were investigated as to their filament forming ability in vitro. from the two main proteins (116000 dalton, 30000 dalton) only the 116000 dalton protein was found to form reversibly distinct filaments of 6-7 nm diameter upon removal of sh-protecting agents from the buffer, whereas the 30000 dalton protein was precipitated as amorphous material under these conditions. the protein filaments were similar to ...197524430373
[changes of glyco- and phospholipid contents during leaf senescence].the glyco- and phospholipid levels in green, green-yellow and yellow leaves of impatients balsamina l., daucus carota l. and cucurbita pepo l. were determined on a leaf area basis. in fagus silvatica l. leaves the kinetics of lipid breakdown during autumnal senescence was analysed. it was shown that yellow tissues still contain 40% to 50% of galactolipids and 50% to 60% of phospholipids present in green tissues. in the early stages of senescence of autumnal fagus leaves we found a more rapid dec ...197624430693
leaf development and phloem transport in cucurbita pepo: maturation of the minor veins.young leaves of cucurbita pepo l. were examined by whole-leaf autoradiography and serial paradermal sections were examined by light microscopy to determine whether commencement of sugar export depends upon the minor vein phloem achieving structural maturity. maturation of these veins develops progressively from the largest toward the smallest elements with the minor veins in the distal region of the leaf maturing before those in the proximal region. commencement of sugar export is coincident wit ...197624430968
particle-bound phytochrome: association with a ribonucleoprotein fraction from cucurbita pepo l.in the absence of ethylenediaminetetraacetic acid (edta) and added mg(2+), the phytochrome, rna, protein, cytochrome c oxidase and nadph-cytochrome c reductase in 20000 x g pellets from hypocotyl hooks of red-irradiated cucurbita seedlings are more or less coincident in a single, broad band on linear sucrose gradients. the inclusion of 3 mm edta in the extraction, resuspension and gradient media has three major effects: (a) the phytochrome profile splits into two main bands; (b) the main rna pop ...197524435122
particle-bound phytochrome: the nature of the interaction between pigment and particulate fractions.particle-bound phytochrome from hypocotyl hooks of cucurbita pepo l. seedlings is associated with either a "heavy" membrane fraction or a 31s ribonucleoprotein (rnp) component on sucrose gradients. those amounts of phytochrome and rnp which co-pellet in response to added mg(2+) are primarily localised in the 31s fraction. the phytochrome-rnp association itself, however, is not dependent on the added cation. this indicates that mg(2+)-enhanced phytochrome pelletability results indirectly from agg ...197524435123
leaf development and phloem transport in cucurbita pepo: carbon economy.net photosynthesis, dark respiration and growth for leaf 5 of cucurbita pepo l. plants grown under controlled conditions were measured and the data used for an assessment of the changes in carbon balance during growth of the leaf through expansion to maturity. the blade is first capable of net co2 fixation when ca. 8% expanded but the initial rapid growth during this period is sustained almost entirely through imported nutrients. when the growth rate starts to decline rapidly the net photosynthe ...197524436024
particle-bound phytochrome: spectral properties of bound and unbound fractions.in-vitro irradiation of extracts of maize (zea mays l.) coleoptiles with red light enhances phytochrome pelletability as was previously reported for cucurbita pepo l. in neither case is this the result of an irradiation-induced increase in the level of total pelletable protein. the transformation difference spectra of bound and unbound phytochrome fractions obtained after both in-vivo and in-vitro irradiations are not significantly different. the data, therefore, do not indicate that irradiation ...197424442378
in-vitro binding of phytochrome to a particulate fraction: a function of light dose and steady-state p fr level.the binding of phytochrome to a particulate fraction in extracts from hypocotyl hooks of etiolated cucurbita pepo l. seedlings has been examined as a function of the light dose and p fr level established in vitro. as the steady-state level of p fr transiently established in the 500×g supernatant is increased, so the level of p r subsequently pelletable at 20 000×g increases up to a saturation level. increasing both the time and irradiance parameters of the light dose while holding the steady-sta ...197424442379
leaf anatomical and photosynthetic acclimation to cool temperature and high light in two winter versus two summer annuals.acclimation of foliar features to cool temperature and high light was characterized in winter (spinacia oleracea l. cv. giant nobel; arabidopsis thaliana (l.) heynhold col-0 and ecotypes from sweden and italy) versus summer (helianthus annuus l. cv. soraya; cucurbita pepo l. cv. italian zucchini romanesco) annuals. significant relationships existed among leaf dry mass per area, photosynthesis, leaf thickness and palisade mesophyll thickness. while the acclimatory response of the summer annuals t ...201424450735
association between photosynthesis and contrasting features of minor veins in leaves of summer annuals loading phloem via symplastic versus apoplastic routes.foliar vascular anatomy and photosynthesis were evaluated for a number of summer annual species that either load sugars into the phloem via a symplastic route (cucumis sativus l. cv. straight eight; cucurbita pepo l. cv. italian zucchini romanesco; citrullus lanatus l. cv. faerie hybrid; cucurbita pepo l. cv. autumn gold) or an apoplastic route (nicotiana tabacum l.; solanum lycopersicum l. cv. brandywine; gossypium hirsutum l.; helianthus annuus l. cv. soraya), as well as winter annual apoplast ...201424450755
temperature dependence of phytochrome dark reactions.the dark reversion and the destruction reaction of the phytochrome of squash (cucurbita pepo l.) cotyledons show different temperature behaviour in the temperature range 15-35°. the arrhenius activation energy of the destruction reaction is temperature independent whereas that of the reversion reaction shows a jump at 20°. this indicates an interaction of phytochrome molecules with membranes. a reaction scheme is discussed which can explain the differences of the dark kinetics in a quantitative ...197424458194
dependence of phytochrome dark reactions on the initial photostationary state.under conditions of continuous irradiation, the p jr destruction rate constants (k d ) of phytochrome in hooks and cotyledons of squash (cucurbita pepo l.) seedlings do not depend on the photostationary state ϕλ and are the same in both organs. on the other hand, the rate constants of the dark reversion and the first destruction step, plotted as a function of ϕ λ (0) , show optimum curves with maxima between ϕ λ (0) and 0.5. similar results were obtained for dark reactions of mustard (sinapis al ...197424458195
relationships between structural development and the absorption of ions by the root system of cucurbita pepo.in both the seminal axis and lateral roots of cucurbita pepo l. the formation of large central xylem elements and the commencement of secondary cambial activity occur 10-20 cm from the root tip. concomitant with or slightly preceding these developments there are changes in the structure of the walls of endodermal cells where the lignified casparian band spreads along the radial wall and substances staining with sudan iv are deposited in both radial and tangential walls. at distances more than 30 ...197324458660
[studies on the phloem exudate of cucurbita pepo l. : i. enzyme activities of aerobic and anaerobic glycolysis and of the citric acid cycle].in the phloem exudate of cucurbita pepo enzyme activities of aerobic and anaerobic glycolysis as well as of citric acid cycle were tested. all enzymes of the aerobic glycolysis and the alcoholic fermentation were present. in the phloem these metabolic pathways should be functioning as well. since the lactate dehydrogenase could not be detected all the time, lactate fermentation is probably not an obligatory constituent of phloem metabolism. of the citric acid cycle the enzymes malate dehydrogena ...197324458663
[studies on the phloem exudate of cucurbita pepo l. : ii. enzyme activities of gluconeogenesis and of the synthesis and degradation of di- and polysaccharides].tests for enzymes of gluconeogenesis and of the synthesis and degradation of sucrose and polysaccharides have been carried out in the phloem exudate of cucurbita pepo. all the enzymes which are necessary for the synthesis of sucrose and polysaccharides from metabolites of the citric acid cycle were found to be present in the exudate, except phosphoenolpyruvate carboxykinase. the polysaccharide synthetase was found to exhibit higher activity with glycogen (which is an unnatural polysaccharide in ...197324458664
thigmomorphogenesis: the response of plant growth and development to mechanical stimulation : with special reference to bryonia dioica.when young plants of hordeum vulgare. bryonia dioica. cucumis sativus. phaseolus vulgaris. mimosa pudica. and ricinus communis. were given a gentle mechanical stimulus by rubbing the internodes for about 10 s once or twice daily, elongation was significantly retarded. plants of cucurbita pepo pisum sativum and triticum aestivum did not exhibit any such response. the initial response to rubbing was very rapid, elongation stopping less than 3 min after application of the stimulus. when the stimulu ...197324458719
changes in carbohydrate content in zucchini fruit (cucurbita pepo l.) under low temperature stress.the postharvest handling of zucchini fruit includes low-temperature storage, making cold stress unavoidable. we have investigated the changes of soluble carbohydrates under this stress and its relation with weight loss and chilling injury in zucchini fruit during postharvest storage at 4 °c and 20 °c for up to 14 days. two varieties with different degrees of chilling tolerance were compared: natura, the more tolerant variety, and sinatra, the variety that suffered more severe chilling-injury sym ...201424467899
leaf development and phloem transport in cucurbita pepo: transition from import to export.the capacity of a growing leaf blade of cucurbita pepo l. to import (14)c-labelled photoassimilate is lost in a basipetal direction. import into the lamina tip stops when the blade is 10% expanded. development of the leaf progresses linearly with time and the lamina base stops importing when the blade is 45% expanded. export capacity also develops basipetally and follows immediately the loss of import capacity, at least in the lamina base. the small amount of material initially exported from the ...197324468909
[localization of dehydrogenases of energy metabolism in the pholoem of cucurbita pepo l].in the stem phloem of cucurbita pepo the enzymes gapdh, adh, mdh, nadp-idh, nad-idh, g6pdh and sdh were localized histochemically with the aid of tetrazolium salt (nbt). when the stems were deep-frozen the most intense formation of formazan was found in companion cells, less in phloem parenchyma cells, and very little in sieve tubes.the distribution of enzymes in phloem markedly changes when stems were cut 2 minutes before freezing: 2,5 cm behind the sectional area little formazan was found. com ...197324469572
[an in vivo study of detectable phytochrome in seeds of cucurbita pepo l. in the course of different phases of germination].in dry gourd seeds all the phytochrome is in the pfr form. the increase of phytochrome content from the beginning of hydration involves two phases, a and b, in the embryonic axis as well as in the cotyledons. cycloheximide does not prevent the appearance of pr during phase a. we assume that pr is gradually released from an inactive complex. on the other hand phase b is inhibited by cycloheximide; this could mean that a de novo synthesis of pr occurs.some experiments indicate that the phytochrome ...197224477896
an investigation of the contractile protein hypothesis of phloem translocation.experiments are reported which were designed to test the hypothesis that the movement of the translocation stream is driven by the contractile activity of p-protein filaments. the different types of filament found after negative staining of phloem exudates from ricinus communis and cucurbita pepo are described. an approximate model is proposed for the quaternary structure of a 20 nm component in the r. communis exudate. none of the filaments showed any ability to bind heavy meromyosin subfragmen ...197224477956
embryoid formation by fragments of cotyledons and hypocotyls in cucurbita pepo.after a prolonged culture on murashige-skoog medium the primary explants of hypocotyls and cotyledons of the pumpkin develop embryoid-producing callus tissue. ten separate strains of such tissue have been obtained and have now been in culture for more than one year, continuing to produce embryoids.197224481562
the conversion of mevalonic acid into gibberellin a12-aldehyde in a cell-free system from cucurbita pepo.a cell-free system prepared from immature seed of cucurbita pepo incorporates the label from mevalonate-2-(14)c into ent-kaur-16-en-19-oic acid (i), ent-7α-hydroxy-kaur-16-en-19-oic acid (ii), and ent-gibberell-16-en-7-al-19-oic acid (iii) (gibberellin a12-aldehyde). the products were identified by gas liquid chromatography and by combined gas chromatography-mass spectrometry of the methylated and trimethylsilylated fractions. the radioactivity of the compounds was established by recrystallisati ...197224482208
[spectrophotometric analysis of phytochrome in the embryo of cucurbita pepo and cucurbita maxima].phytochrome detection and △ (△o.d.) measurements were made for the first time in a single embryo of pumpkin or gourd thanks to an apparatus adjusted to the differential spectrophotometer constructed by dr. spruit (wageningen). observed △ (△o.d.) values are comparable with usual values obtained by analysis of several seeds in a cuvette. the differential spectrum obtained has the same characteristics as the spectra of seedlings and seeds which can be found in the present literature. the time cours ...197024499981
the enzymic preparation of (14)c-kaurene.endosperm from immature seeds of cucurbita pepo l. converts 2-(14)c-dl-mevalonate to (14)c-(-)-kaurene with a yield of nearly 40% of the active isomer. kaurene is the main product and the only diterpene hydrocarbon which is formed from mevalonate in the system and is therefore easily obtained radiochemically pure. the product was identified by thin-layer chromatography and recrystallization with authentic (-)-kaurene to constant specific radioactivity.196924515589
[identification of tritiated assimilales within the sieve tubes of cucurbita pepo].after application of d-glucose-6-t to small areas of leaf surfaces of cucurbita, radioactivity - mainly sucrose-t - could exclusively be localized by histoautoradiography ("dry-technique") within single sieve tubes of exporting bundles. the centre of activity corresponded to the "slime"-plugs (in crosssection-autoradiograms) of the sieve tubes and to the sieve plates (in longitudinal sections). it is concluded that only the sieve tubes are the conducting channels for the long-distance transport ...196824519078
evaluation of six plant species used traditionally in the treatment and control of diabetes mellitus in south africa using in vitro methods.numerous plants are used by the local communities of south africa for the treatment and management of type ii diabetes.201424559378
effects of virus on plant fecundity and population dynamics.microorganisms are ubiquitous and thought to regulate host populations. although microorganisms can be pathogenic and affect components of fitness, few studies have examined their effects on wild plant populations. as individual traits might not contribute equally to changes in population growth rate, it is essential to examine the entire life cycle to determine how microorganisms affect host population dynamics. in this study, we used data from common garden experiments with plants from three c ...201424571200
molecular and functional characterization of cpacs27a gene reveals its involvement in monoecy instability and other associated traits in squash (cucurbita pepo l.).a number of cucurbita pepo genotypes showing instable monoecy or partial andromonoecy, i.e. an incomplete conversion of female into bisexual flowers, have been detected. given that in melon and cucumber andromonoecy is the result of reduction of ethylene production in female floral buds, caused by mutations in the ethylene biosynthesis genes cmacs7 and csacs2; we have cloned and characterized two related c. pepo genes, cpacs27a and cpacs27b. the molecular structure of cpacs27a and its specific e ...201424595516
two and three dimensional characterization of zucchini yellow mosaic virus induced structural alterations in cucurbita pepo l. plants.infection of plants by zucchini yellow mosaic virus (zymv) induces severe ultrastructural changes. the aim of this study was to investigate ultrastructural changes during zymv-infection in cucurbita pepo l. plants on the two and three dimensional (2d and 3d) level and to correlate these changes with the spread of zymv throughout the plant by transmission electron microscopy (tem) and image analysis. this study revealed that after inoculation of the cotyledons zymv moved into roots [3 days post i ...201424631670
optimization of preparation of antioxidative peptides from pumpkin seeds using response surface method.protein isolates of pumpkin (cucurbita pepo l) seeds were hydrolyzed by acid protease to prepare antioxidative peptides. the hydrolysis conditions were optimized through box-behnken experimental design combined with response surface method (rsm). the second-order model, developed for the dpph radical scavenging activity of pumpkin seed hydrolysates, showed good fit with the experiment data with a high value of coefficient of determination (0.9918). the optimal hydrolysis conditions were determin ...201424637721
determining the mineral composition in cucurbita pepo fruit using near infrared reflectance spectroscopy.efforts through conventional breeding to improve the mineral content in horticultural crops have not always been successful mainly due to the fact that standard analytical methods are both costly and time-consuming. we investigated the feasibility of applying near infrared reflectance spectroscopy (nirs) to the estimation of essential mineral composition in the skin and flesh of summer squash fruits (cucurbita pepo subsp. pepo) using a 200-sample set from diverse morphotypes.201424652716
characterization of conserved micrornas from five different cucurbit species using computational and experimental analysis.micrornas (mirnas) are ∼21 nt non-coding small rnas which regulate gene expression at the post-transcriptional level in plants and animals. until recently, only limited numbers of mirnas were identified in cucurbitaceae, a large flowering plant family. in this study, 220 potential mirna candidates were identified from five species of cucurbitaceae family using a comparative genome-based computational analysis. a comprehensive bioinformatic analysis of est (expressed sequence tag) and gss (genomi ...201424657600
evidence of local adaptation in plant virus effects on host-vector interactions.recent research suggests that plant viruses, and other pathogens, frequently alter host-plant phenotypes in ways that facilitate transmission by arthropod vectors. however, many viruses infect multiple hosts, raising questions about whether these pathogens are capable of inducing transmission-facilitating phenotypes in phylogenetically divergent host plants and the extent to which evolutionary history with a given host or plant community influences such effects. to explore these issues, we worke ...201424748599
aphrodisiac activity of polyherbal formulation in experimental models on male rats.to investigate the aphrodisiac potential of polyherbal formulations prepared from different parts of tribulus terrestris, curculigo orchioides, allium tuberosum, cucurbita pepo, elephant creeper, mucuna pruriens, and terminalia catappa in albino rats in specified ratio as suspension.201424761115
fibre from pumpkin (cucurbita pepo l.) seeds and rinds: physico-chemical properties, antioxidant capacity and application as bakery product ingredients.the aims of this study were to determine the proximate composition, functional properties and antioxidant activity of pumpkin seeds and rind. besides, the effects of dietary fibre in pumpkin seeds and rinds on bread qualities and properties were evaluated.201324800388
pumpkin seed oil extracted from cucurbita maxima improves urinary disorder in human overactive bladder.the pumpkin seed oil obtained from cucurbita pepo has been shown to be useful for the treatment of nocturia in patients with urinal disorders in several western countries. in this study, we evaluated the effect of the pumpkin seed oil from cucurbita maxima on urinary dysfunction in human overactive bladder (oab). forty-five subjects were enrolled in this study. an extract of pumpkin seed oil from c. maxima (10 g of oil/day) was orally administrated for 12 weeks. after 6 and 12 weeks, urinary fun ...201424872936
the volatile emission of eurosta solidaginis primes herbivore-induced volatile production in solidago altissima and does not directly deter insect feeding.the induction of plant defenses in response to herbivory is well documented. in addition, many plants prime their anti-herbivore defenses following exposure to environmental cues associated with increased risk of subsequent attack, including induced volatile emissions from herbivore-damaged plant tissues. recently, we showed in both field and laboratory settings that tall goldenrod plants (solidago altissima) exposed to the putative sex attractant of a specialist gall-inducing fly (eurosta solid ...201424947749
contribution of polyamines and other related metabolites to the maintenance of zucchini fruit quality during cold storage.in order to investigate the contribution of polyamines and related amino acids in the maintenance of zucchini fruit quality during cold storage, two varieties of cucurbita pepo with different degrees of chilling tolerance were used, natura (more tolerant) and sinatra (moresensitive). after harvest, free putrescine levels decreased during storage at 20 °c, whereas in fruit kept at 4 °c this polyamine accumulated in both varieties, but with higher levels in the sensitive variety (sinatra). this be ...201424960300
bacterial degradation of aroclor 1242 in the mycorrhizosphere soils of zucchini (cucurbita pepo l.) inoculated with arbuscular mycorrhizal fungi.a greenhouse experiment was conducted to investigate the effects of zucchini (cucurbita pepo l.), inoculated with the arbuscular mycorrhizal (am) species acaulospora laevis, glomus caledonium, and glomus mosseae, on the soil bacterial community responsible for aroclor 1242 dissipation. the dissipation rates of aroclor 1242 and soil bacteria abundance were much higher with the a. laevis and g. mosseae treatments compared to the non-mycorrhizal control. the biphenyl dioxygenase (bpha) and rhodococ ...201424969432
use of visible and near-infrared spectroscopy for predicting antioxidant compounds in summer squash (cucurbita pepo ssp pepo).the food industry and plant breeding programmes require fast, clean and low-cost screening techniques for nutritional compounds determination in food matrices. this is the first report on the study of the potential of near-infrared spectroscopy (nirs) for the prediction of antioxidant compounds in summer squash tissues collected since 2009-2012. modified partial least-squares (mpls) regression was used to correlate spectral information and the different antioxidant compounds in the samples. the ...201424996338
nano-silicon dioxide mitigates the adverse effects of salt stress on cucurbita pepo l.research into nanotechnology, an emerging science, has advanced in almost all fields of technology. the aim of the present study was to evaluate the role of nano-silicon dioxide (nano-sio2 ) in plant resistance to salt stress through improvement of the antioxidant system of squash (cucurbita pepo l. cv. white bush marrow). seeds treated with nacl showed reduced germination percentage, vigor, length, and fresh and dry weights of the roots and shoots. however, nano-sio2 improved seed germination a ...201425066835
analysis of viral (zucchini yellow mosaic virus) genetic diversity during systemic movement through a cucurbita pepo vine.determining the extent and structure of intra-host genetic diversity and the magnitude and impact of population bottlenecks is central to understanding the mechanisms of viral evolution. to determine the nature of viral evolution following systemic movement through a plant, we performed deep sequencing of 23 leaves that grew sequentially along a single cucurbita pepo vine that was infected with zucchini yellow mosaic virus (zymv), and on a leaf that grew in on a side branch. strikingly, of 112 g ...201425107623
phytotherapy of benign prostatic hyperplasia. a minireview.benign prostate hyperplasia (bph) is a common condition affecting older men, with an incidence that is age-dependent. histological bph, which typically develops after the age of 40 years, ranges in prevalence from >50% at 60 years to as high as 90% by 85 years of age. typical symptoms include increased frequency of urination, nocturia, urgency, hesitancy, and weak urine stream. conventional medicines used for the treatment of bph include alpha blockers and 5-alpha reductase inhibitors. this arti ...201425165780
evaluating pollination deficits in pumpkin production in new york.potential decreases in crop yield from reductions in bee-mediated pollination services threaten food production demands of a growing population. many fruit and vegetable growers supplement their fields with bee colonies during crop bloom. the extent to which crop production requires supplementary pollination services beyond those provided by wild bees is not well documented. pumpkin, cucurbita pepo l., requires bee-mediated pollination for fruit development. previous research identified the comm ...201425198126
new vitamin e isomers (gamma-tocomonoenol and alpha-tocomonoenol) in seeds, roasted seeds and roasted seed oil from the slovenian pumpkin variety 'slovenska golica'.the štajerska region in north-eastern slovenia and the styria region in southern austria have a long tradition of growing pumpkins (cucurbita pepo l.) as an oil crop. gc-ms determination of the free and esterified minor compounds in oil of roasted pumpkin seeds from the slovenian c. pepo l. variety 'slovenska golica' revealed the presence of two previously unreported compounds: alpha-tocomonoenol and gamma-tocomonoenol. using the gc-ms data, reference samples (crude palm oil) and tocopherol and ...201125212163
occurrence of squash yellow mild mottle virus and pepper golden mosaic virus in potential new hosts in costa rica.leaf samples of solanum lycopersicum, capsicum annuum, cucurbita moschata, cucurbita pepo, sechium edule and erythrina spp. were collected. all samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. a sequence of ∼1,100 bp was obtained from the genomic component dna-a of 14 samples. in addition, one sequence of ∼580 bp corresponding to the coat protein (av1) was obtained from a chayote (s. edule) leaf sample. the presence of squash yellow mild mottle viru ...201325288955
first report of cucumber mosaic virus isolated from wild vigna angularis var. nipponensis in korea.a viral disease causing severe mosaic, necrotic, and yellow symptoms on vigna angularis var. nipponensis was prevalent around suwon area in korea. the causal virus was characterized as cucumber mosaic virus (cmv) on the basis of biological and nucleotide sequence properties of rnas 1, 2 and 3 and named as cmv-wva. cmv-wva isolate caused mosaic symptoms on indicator plants, nicotiana tabacum cv. xanthi-nc, petunia hybrida, and cucumis sativus. strikingly, cmv-wva induced severe mosaic and malform ...201425289004
particle-size dependent accumulation and trophic transfer of cerium oxide through a terrestrial food chain.the accumulation and trophic transfer of nanoparticle (np) or bulk ceo2 through a terrestrial food chain was evaluated. zucchini (cucurbita pepo l.) was planted in soil with 0 or 1228 μg/g bulk or np ceo2. after 28 d, zucchini tissue ce content was determined by icp-ms. leaf tissue from each treatment was used to feed crickets (acheta domesticus). after 14 d, crickets were analyzed for ce content or were fed to wolf spiders (family lycosidae). np ceo2 significantly suppressed flower mass relativ ...201425340623
carbohydrate metabolism before and after dehiscence in the recalcitrant pollen of pumpkin (cucurbita pepo l.).pumpkin (cucurbita pepo l.) pollen is starchy, sucrose-poor and recalcitrant, features opposite to those of several model species; therefore, some differences in carbohydrate metabolism could be expected in this species. by studying pumpkin recalcitrant pollen, the objective was to provide new biochemical evidence to improve understanding of how carbohydrate metabolism might be involved in pollen functioning in advanced stages. four stages were analysed: immature pollen from 1 day before anthesi ...201525353653
protective effect of cucurbita pepo fruit peel against ccl4 induced neurotoxicity in rat.cucurbita pepo is a common vegetable used all over the world. in folk medicine it is used in gastroenteritis, hepatorenal and in brain anomalies. in the present study, protective effect of cucurbita pepo fruit peel against ccl4-induced neurotoxicity in rats was investigated. in this study, 36 sprague-dawley female rats (190±15 g) were randomly divided into 6 groups of 6 rats each. group i was given 1 ml/kg bw (body weight) of corn oil intraperotoneally (i.p); group ii, iii and iv were treated wi ...201425362619
first tilling platform in cucurbita pepo: a new mutant resource for gene function and crop improvement.although the availability of genetic and genomic resources for cucurbita pepo has increased significantly, functional genomic resources are still limited for this crop. in this direction, we have developed a high throughput reverse genetic tool: the first tilling (targeting induced local lesions in genomes) resource for this species. additionally, we have used this resource to demonstrate that the previous ems mutant population we developed has the highest mutation density compared with other cu ...201425386735
differential uptake and translocation of β-hch and dieldrin by several plant species from hydroponic medium.to compare the uptake and translocation of hydrophobic organic chemicals by plant species, the authors performed uptake experiments with β-1,2,3,4,5,6-hexachlorocyclohexane (β-hch) and 1,2,3,4,10,10-hexachloro-6,7-epoxy-1,4,4a,5,6,7,8,8a-octahydro-endo-1,4-exo-5,8-dimethanonaphthalene (dieldrin) using 5 species: hordeum vulgare, glycine max, solanum lycopersicum, brassica oleracea, and cucurbita pepo. the present study evaluated uptake ability using root concentration factor (rcf) and translocat ...201525470472
zinc finger protein genes from cucurbita pepo are promising tools for conferring non-cucurbitaceae plants with ability to accumulate persistent organic pollutants.some cultivars of cucumbers, melons, pumpkins, and zucchini, which are members of the cucurbitaceae family, are uniquely subject to contamination by hydrophobic pollutants such as the organohalogen insecticides ddt. however, the molecular mechanisms for the accumulation of these pollutants in cucurbits have not been determined. here, cdna subtraction analysis of cucurbita pepo cultivars that are low and high accumulators of hydrophobic contaminants revealed that a gene for zinc finger proteins ( ...201525532761
investigating differences in the root to shoot transfer and xylem sap solubility of organic compounds between zucchini, squash and soybean using a pressure chamber method.a pressure chamber method was used to examine differences in the root to shoot transfer and xylem sap solubility of caffeine (log kow=-0.07), triclocarban (log kow=3.5-4.2) and endosulfan (log kow=3.8-4.8) for zucchini (cucurbita pepo ssp pepo), squash (cucurbita pepo ssp ovifera), and soybean (glycine max l.). transpiration stream concentration factors (tscf) for caffeine (tscf=0.8) were statistically equivalent for all plant species. however, for the more hydrophobic endosulfan and triclocarba ...201525537866
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