Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| nitrile hydratase gene from rhodococcus sp. n-774 requirement for its downstream region for efficient expression. | for improvement of the production of nitrile hydratase (nhase) from rhodococcus sp. n-774 by recombinant dna techniques, several plasmids, each of which had a deletion of the upstream or downstream region of the genes encoding the alpha and beta subunits of nhase, were constructed. enzyme assays of recombinant r. rhodochrous and escherichia coli cells showed that a downstream region of the nhase genes was indispensable for the production of active nhase in both cells, but for the production of t ... | 1994 | 7765511 |
| radiologic findings in two aids patients with rhodococcus equi pneumonia. | rhodococcus equi (r. equi) has been reported as an occasional cause of pulmonary infection in severely immunocompromised hosts, including aids patients. our purpose is to describe the radiologic findings in two aids patients with r. equi pneumonia. chest radiographs showed right-upper-lobe consolidation and cavitation in both patients. chest ct confirmed upper mediastinal involvement and precarinal lymphadenopathy in both cases. multiple lung nodules related to the bronchi were also identified i ... | 1995 | 7769626 |
| metabolism of halohydroquinones in rhodococcus chlorophenolicus pcp-1. | the actinomycete rhodococcus chlorophenolicus pcp-1 metabolizes pentachlorophenol into ultimate inorganic end products via tetrachloro-p-hydroquinone. this intermediate was further dehalogenated in the cytoplasm requiring reductant in the cell free system. tetrafluoro-p-hydroquinone and tetrabromo-p-hydroquinone were also dehalogenated. chlorophenol analogs, thiol blocking agents and molecular oxygen inhibited the activity. the dehalogenating reactions led to 1,2,4-trihydroxybenzene, which was f ... | 1995 | 7772938 |
| rhodococcal abortion and pneumonia in an equine fetus. | 1995 | 7779954 | |
| distribution and characterization of beta-lactamases of mycobacteria and related organisms. | the detailed distribution and precise features of mycobacterial beta-lactamases urgently need to be elucidated. | 1995 | 7780097 |
| what is your diagnosis? osteomyelitis, septic arthritis. | 1995 | 7782239 | |
| characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader rhodococcus sp. strain rha1. | rhodococcus sp. strain rha1 is a gram-positive polychlorinated biphenyl (pcb) degrader which can degrade 10 ppm of pcb48 (equivalent to aroclor1248), including tri-, tetra-, and pentachlorobiphenyls, in a few days. we isolated the 7.6-kb ecori-bamhi fragment carrying the biphenyl catabolic genes of rha1 and determined their nucleotide sequence. on the basis of deduced amino acid sequence homology, we identified six bph genes, bpha1a2a3a4, bphb, and bphc, that are responsible for the initial thre ... | 1995 | 7793929 |
| active-site analysis of ferric p450 enzymes: hydrogen-bonding effects on the circular dichroism spectra. | active-site cd spectra were analyzed for p450's with known x-ray structures (p450terp, p450bm-3, p450cam). cd spectral patterns for fe(3+)-substrate-free p450's reflect structure/function properties of the distal pocket. p450terp and p450bm-3 have an h-bond between 6th ligand and i-helix [hasemann, c.a., et al. (1995) structure, 3, 41-62], and the soret cd band at approximately 410 nm is approximately 2-fold larger than that at approximately 350 nm. for p450cam, the two cd bands are more nearly ... | 1995 | 7794248 |
| biochemical and serological characteristics of rhodococcus equi isolates from animals and humans. | in the present study, 17 rhodococcus equi isolates from animals and 13 r. equi isolates from humans were correctly identified by the use of the api coryne-test system. the biochemical characteristics revealed no significant differences between isolates from animals and humans. heat extractions at acid ph or autoclave extractions of the bacteria and the use of monospecific antisera against type antigens 1-7 allowed the serotyping of the bacteria. the majority of the r. equi isolates reacted with ... | 1994 | 7801718 |
| [rhodococcus equi pneumonia in patients with immunodeficiency]. | in three patients, men of 30, 21 and 24 years old respectively, rhodococcus equi pneumonia was diagnosed. antibiotic treatment, followed by secondary prophylaxis, was successful. in one patient, lobectomy was done. r. equi, a well-known bacterial pathogen in horses, appears also to be pathogenic in humans in case of immunodeficiency. | 1994 | 7808528 |
| [rhodococcus equi pneumonia in a patient with aids. primary resistance to imipenem]. | 1994 | 7811779 | |
| interaction of virulent and non-virulent rhodococcus equi human isolates with phagocytes, fibroblast- and epithelial-derived cells. | rhodococcus equi is a facultative, intracellular, gram-positive coccobacillus, increasingly reported in pneumonia of aids-infected patients. we investigated killing resistance properties of human r. equi virulent and avirulent human strains. avirulent beta-lactam-susceptible strains had lower intracellular colony forming units after 45 min incubation in murine macrophages j774 and human monocyte-macrophage tph-1 than those of virulent strains. only virulent beta-lactam-resistant strains persiste ... | 1994 | 7812268 |
| chlorocatechol 1,2-dioxygenase from rhodococcus erythropolis 1cp. kinetic and immunochemical comparison with analogous enzymes from gram-negative strains. | chlorocatechol 1,2-dioxygenase from rhodococcus erythropolis 1cp was purified to homogeneity. in contrast to chlorocatechol 1,2-dioxygenase from gram-negative strains which have a very broad substrate tolerance, the rhodococcus enzyme was relatively more specific and had a distinct preference for 4-substituted catechols. protein and metal analysis indicate an unusual stoichiometry of one atom each of iron and manganese/64-kda homodimer. the n-terminal amino acid sequence (27 residues) of the rho ... | 1994 | 7813460 |
| opacification of middlebrook agar as an aid in distinguishing nocardia farcinica within the nocardia asteroides complex. | among 58 aerobic actinomycetes isolated from different sources and geographical locations, none of 23 nocardia asteroides isolates, at 18 n. farcinica isolates, 1 of 5 n. otitidiscaviarum isolates, and 1 of 4 rhodococcus species isolates opacified middlebrook 7h10 medium. within the n. asteroides complex, this characteristic, together with growth at 45 degrees c and resistance to each of erythromycin, cefotaxime, and tobramycin, provides a simple means of distinguishing n. farcinica from n. aste ... | 1994 | 7814557 |
| photosensitive nitrile hydratase intrinsically possesses nitric oxide bound to the non-heme iron center: evidence by fourier transform infrared spectroscopy. | nitrile hydratase (nhase) from rhodococcus sp. n-771 is a photosensitive enzyme that catalyzes hydration of nitriles to the corresponding amides. light-induced fourier transform infrared difference spectra between the inactive and active forms of nhase were measured with both the natural (14n) and 15n-labeled nhases. the results showed, for the first time, that nhase intrinsically possesses nitric oxide (no) molecules bound to the non-heme iron center. the possible role of no in the photoactivat ... | 1995 | 7821438 |
| primary subcutaneous abscess caused by rhodococcus equi. | 1995 | 7825776 | |
| [rhodococcus equi pneumonia in patients with aids]. | 1994 | 7827773 | |
| degradation of the thiocarbamate herbicide eptc (s-ethyl dipropylcarbamothioate) and biosafening by rhodococcus sp. strain ni86/21 involve an inducible cytochrome p-450 system and aldehyde dehydrogenase. | determination of the n-terminal sequences of two eptc (s-ethyl dipropylcarbamothioate)-induced proteins from thiocarbamate-degrading rhodococcus sp. strain ni86/21 resolved by two-dimensional electrophoresis enabled the localization of the respective structural genes on two distinct dna fragments. one of these strongly induced proteins is a nad(+)-dependent dehydrogenase active on aliphatic aldehydes. the second protein was identified as a cytochrome p-450 enzyme. the cytochrome p-450 gene repre ... | 1995 | 7836301 |
| rhodococcus equi infection in hiv-positive patients: report of 5 cases and literature overview. | the importance of rhodococcus equi infection, an uncommon human pathogen that almost exclusively affects immunocompromised hosts, has greatly increased following the advent of acquired immune deficiency syndrome (aids) epidemics. until the present time, 38 cases of r. equi infection have been described in human immunodeficiency virus (hiv)-infected patients; we now report a further five personal cases. r. equi was acquired via respiratory exposure to animals in less than half of the patients, an ... | 1994 | 7841972 |
| cytologic appearance of rhodococcus equi in bronchoalveolar lavage specimens. a case report. | immunocompromised patients are at risk of developing infections caused by rhodococcus equi, a gram-positive bacillus that can cause pneumonia in patients with acquired immunodeficiency syndrome. to the author's knowledge this is the first report describing the cytologic features of the infection in a patient who had a confirmatory tissue biopsy and positive culture. infection with r equi may go unrecognized by pathologists unaware of its presentation and appearance in cytologic material. | 1995 | 7846998 |
| rhodococcus equi: fatal pneumonia in a patient without aids. | rhodococcus equi infections are being recognized with greater frequency in immunocompromised patients and the population infected with the human immunodeficiency virus (hiv). r. equi usually causes a progressive pneumonia that may or may not cavitate and is relatively refractory to antibiotic therapy. we report a case of fatal r. equi pneumonia in a normal host. | 1994 | 7852068 |
| routine identification of mycobacterium tuberculosis complex isolates by automated hybridization. | methodologies for biochemical identification of mycobacteria isolated from clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. we describe here a 2-h identification system for mycobacterial isolates belonging to the mycobacterium tuberculosis complex using a dna probe. after 30 min of hands-off sample preparation, the 1.5-h hybridization test is totally automated in the newly developed vidas system (biomérieux, marcyl'etoile, france), which performs solid-phase specif ... | 1994 | 7852560 |
| amino acid sequence analysis of ribosomal protein at-l30 from members of the family pseudonocardiaceae. | the phylogenetic relationships of the genera belonging to the family pseudonocardiaceae were examined by a novel approach, amino acid sequencing of ribosomal at-l30 proteins. the results of partial amino acid sequencing of at-l30 preparations revealed that the members of the family pseudonocardiaceae are divided into four clusters; the first cluster contains the genus actinopolyspora, the second cluster contains the genus saccharopolyspora, the third cluster contains the genus amycolatopsis, and ... | 1995 | 7857791 |
| dietzia, a new genus including dietzia maris comb. nov., formerly rhodococcus maris. | sequencing of the 16s ribosomal dnas (rdna) of two strains of rhodococcus maris was performed to determine the relationship of this species to other mycolic acid-containing actinomycetes. for this purpose we also determined the 16s rdna sequences for the type species of the genus rhodococcus, rhodococcus rhodochrous, and for mycobacterium chlorophenolicum (formerly rhodococcus chlorophenolicus), rhodococcus erythropolis, gordona bronchialis, and gordona terrae, for which only partial sequence da ... | 1995 | 7857805 |
| enzyme-catalysed enantioselective hydrolysis of racemic naproxen nitrile. | the bacterial strain rhodococcus butanica (atcc 21197), which exhibits nitrilase and nitrile hydratase/amidase activities, catalyses the enantioselective hydrolysis of racemic naproxen nitrile (r/s)-1 to furnish a moderate enantiomeric excess of (s)-naproxen (s)-3. racemic naproxen amide (r/s)-2 is not a good substrate for this strain. resting cells of the newly selected bacterial strain rhodococcus sp. c3ii catalyse the enantioselective hydrolyses of racemic naproxen nitrile (r/s)-1 and naproxe ... | 1994 | 7858980 |
| o-, m- and p-hydroxybenzoate degradative pathways in rhodococcus erythropolis. | rhodococcus erythropolis strain s1 uses the gentisate pathway to metabolize salicylate and m-hydroxybenzoate and the protocatechuate pathway to degrade p-hydroxybenzoate. m-hydroxybenzoate 6-hydroxylase was induced by growth on m-hydroxybenzoate or gentisate, and salicylate 5-hydroxylase only by growth on salicylate. p-hydroxybenzoate 3-hydroxylase could be induced only by growth on p-hydroxybenzoate. m-hydroxybenzoate or p-hydroxybenzoate could repress the induction of salicylate 5-hydroxylase. ... | 1995 | 7867918 |
| brevibacterium linens pbl33 and rhodococcus rhodochrous prc1 cryptic plasmids replicate in rhodococcus sp. r312 (formerly brevibacterium sp. r312). | the replication of two cryptic plasmids from brevibacterium linens atcc 9174 (pbl33) and rhodococcus rhodochrous atcc 4276 (prc1) was investigated in rhodococcus sp. r312 (formerly brevibacterium sp. r312). the recombinant plasmids psp33 (pbl33 derivative) and pspc1 (prc1 derivative) were found to be suitable for establishing new host-vector systems for rhodococcus sp. r312. they all carry the tn903 neomycin-resistance-encoding gene (aphi). | 1995 | 7867954 |
| isolation of insertion elements from gram-positive brevibacterium, corynebacterium and rhodococcus strains using the bacillus subtilis sacb gene as a positive selection marker. | the sacb gene of bacillus subtilis was successfully applied in various arthrobacter, brevibacterium, corynebacterium and rhodococcus strains for the isolation of transposable elements. three different insertion sequence (is) elements entrapped in sacb were isolated. the is elements is-bl and is-cg isolated from brevibacterium lactofermentum and corynebacterium glutamicum, respectively, were found to be similar in size (1.45 kb) and generated target duplications of 8 bp. their inverted repeats sh ... | 1995 | 7896070 |
| [distribution of alpha-n-acetylgalactosaminidases in marine and freshwater microorganisms]. | the distribution of the alpha-n-acetylgalactosidases producers among 856 marine bacteria isolated from sea water, sediments and associated with sponges, mollusks, echinoderms inhabited in different area of the pacific ocean and 423 baikalian microorganisms from fresh water and sponges have been studied. about 24% as marine as baikalian strains produced this enzyme. the most number of alpha-n-ac-galactosidase producers were found for marine bacteria genera aeromonas, alteromonas and vibrio (71% a ... | 1994 | 7898396 |
| [nosocomial infections: new agents, incidence, prevention]. | nosocomial infection is one of the major health problems confronting clinicians in intensive care units. incidence has been estimated to vary from 5 to 10%, leading to death in one of every 5000 persons hospitalized. this situation is not however static since hospital epidemics and nosocomial infections evolve as a consequence of advances in antibiotic drug therapy and surgical and intensive care techniques. microbial investigations themselves are of major importance as the collection of epidemi ... | 1995 | 7899352 |
| amidase coupled with low-molecular-mass nitrile hydratase from rhodococcus rhodochrous j1. sequencing and expression of the gene and purification and characterization of the gene product. | the cloned 9.4-kb insert of plasmid pnhj20l containing low-molecular-mass nitrile hydratase (l-nhase) gene from rhodococcus rhodochrous j1 [kobayashi, m. et al. (1991) biochim. biophys. acta 1129, 23-33] was digested with various restriction enzymes, and the trimmed fragments were inserted into puc18 or puc19. a 1.96-kb ecori-sphi region located 1.9-kb downstream of the l-nhase gene was found to be essential for the expression of amidase activity in escherichia coli; the gene arrangement of the ... | 1993 | 7916690 |
| bacteria and pesticides: a new aspect of interaction--involvement of a new biofactor. | positively charged hydrophobic pesticides of the dipyridyl family [diquat, paraquat, benzylviologen (bv++), etc.] were shown to provoke accumulation of 2-methylbutane-1,2,3,4-tetraol-2,4- cyclopyrophosphate in the cells corynebacterium (brevibacterium) ammoniagenes while neutral dipyridyls were not. hydrophobicity was also an important factor in this phenomenon. of the other pesticides tested, only linuron was effective. bv++ also induced biosynthesis of the compound in rhodococcus rhodochrous, ... | 1994 | 7916959 |
| detection of a genetic locus encoding resistance to rifampin in mycobacterial cultures and in clinical specimens. | the polymerase chain reaction (pcr) and automated dna sequencing were used to detect a genetic locus, rpob, associated with rifampin resistance in mycobacterium tuberculosis (tb) in clinical isolates and directly in clinical specimens. primers derived from the sequence of a tb rpob gene fragment were used to amplify dna from bacterial and mycobacterial isolates. an rpob-specific pcr product was obtained for five of five tb, seven of eight other mycobacterial species, nocardia sp., corynebacteriu ... | 1994 | 7924218 |
| tamponade due to rhodococcus equi in acquired immunodeficiency syndrome. | infection with rhodococcus equi has been described as a cause of cavitary pneumonia in aids patients. we report such a case, complicated by bacteremia, tamponade, and possible brain and liver abscesses. medical treatment was successful with pericardiocentesis and antibiotherapy with teicoplanin, gentamicin, clarithromycin, rifampicin, supplemented by empirical treatment of cerebral toxoplasmosis. antibiotherapy was terminated after 6 months, without relapse 2 months later. | 1994 | 7924515 |
| survival and replication of rhodococcus equi in macrophages. | rhodococcus equi is a facultative intracellular bacterium of macrophages that can cause serious pneumonia in both young horses and immunocompromised people. essential to understanding rhodococcus pathogenesis is a quantitative documentation of the intracellular events that follow macrophage phagocytosis of the organism. by using a bacterial immunofluorescence staining assay, we verified the intracellular survival and replicative potential of r. equi in both murine peritoneal macrophages and equi ... | 1994 | 7927672 |
| phosphorylative inactivation of rifampicin by nocardia otitidiscaviarum. | rifampicin was inactivated by nocardia otitidiscaviarum to yield two products designated rip-3 and rip-4. the full structures of rip-3 and rip-4 were determined to be 21-(o-phosphoryl) rifampicin and 3-formyl-21-(o-phosphoryl) rifamycin sv, respectively, but neither product had any antimicrobial activity. | 1994 | 7928806 |
| [new macrolides]. | the macrolides are still widely used in general practice and the new 14- or 15 membered-ring compounds-roxithromycin, clarithromycin, dirithromycin, azithromycin -may offer a new development. their antibacterial activity is similar to erythromycin but azithromycin is more active against gram negative strams. roxythromycin exhibits high serum concentrations; azithromycin and dirithromycin present low serum concentrations but prolonged half-lives allowing a reduction of the duration of treatment p ... | 1994 | 7939238 |
| pulmonary malacoplakia associated with rhodococcus equi infection in a patient with acquired immunodeficiency syndrome. | pulmonary malacoplakia associated with rhodococcus equi, an opportunistic gram-positive coccobacillus, is unusual. although this patient is the ninth reported to have pulmonary malacoplakia, he is only the third patient with the acquired immunodeficiency syndrome who has been reported to have pulmonary malacoplakia associated with r equi infection. the patient was a 49-year-old man infected with the human immunodeficiency virus who on initial examination was found to have cavitary pneumonia in t ... | 1994 | 7942603 |
| purification and characterization of an amidase from an acrylamide-degrading rhodococcus sp. | a constitutively expressed aliphatic amidase from a rhodococcus sp. catalyzing acrylamide deamination was purified to electrophoretic homogeneity. the molecular weight of the native enzyme was estimated to be 360,000. upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified preparation yielded a homogeneous protein band having an apparent molecular weight of about 44,500. the amidase had ph and temperature optima of 8.5 and 40 degrees c, respectively, and its isoelectric poin ... | 1994 | 7944367 |
| the enzymes of beta-oxidation in rhodococcus ruber. | 1994 | 7958285 | |
| bacterial oxidation of propane. | the bacterial metabolism of propane and the pathway(s) involved are poorly understood, as the relative importance of terminal versus subterminal oxidation of propane, via propan-1-ol and propan-2-ol, respectively, is still unclear. in the case of bacteria, the ability to oxidize propane appears to be confined mainly to the gram-positive corynebacterium - nocardia - mycobacterium - rhodococcus complex. studies on propane oxidation have been hampered by a lack of firm enzymological data; for examp ... | 1994 | 7958761 |
| characterization of the desulfurization genes from rhodococcus sp. strain igts8. | rhodococcus sp. strain igts8 possesses an enzymatic pathway that can remove covalently bound sulfur from dibenzothiophene (dbt) without breaking carbon-carbon bonds. the dna sequence of a 4.0-kb bstbi-bsiwi fragment that carries the genes for this pathway was determined. frameshift and deletion mutations established that three open reading frames were required for dbt desulfurization, and the genes were designated soxabc (for sulfur oxidation). each sox gene was subcloned independently and expre ... | 1994 | 7961424 |
| evaluation of api coryne system for identifying coryneform bacteria. | to identify rapidly and accurately coryneform bacteria, using a commercial strip system. | 1994 | 7962633 |
| [serodiagnosis of caseous lymphadenitis (pseudotuberculosis) in sheep]. | random postmortal isolation of corynebacterium pseudotuberculosis from the sheep used in a research project and the consequently determined serological positivity of these animals indicated the serological examination of the flock from which the sheep came. no symptoms of caseous lymphadenitis were ever observed in the flock in question and no data on the occurrence of this disease were available in the flock history. the sera of 228 head of sheep were used for examinations, while this number of ... | 1994 | 7975051 |
| transfer of rhodococcus aichiensis tsukamura 1982 and nocardia amarae lechevalier and lechevalier 1974 to the genus gordona as gordona aichiensis comb. nov. and gordona amarae comb. nov. | we investigated the taxonomic status of rhodococcus aichiensis dsm 43978t (t = type strain) and nocardia amarae dsm 43392t by using both chemotaxonomic and genetic methods. the occurrence of mk-9(h2) as the predominant menaquinone, the presence of relatively long-chain mycolic acids (48 to 62 carbon atoms), and the phylogenetic position as determined by a 16s ribosomal dna sequence analysis provide strong evidence that both r. aichiensis and n. amarae should be transferred to the genus gordona a ... | 1994 | 7981103 |
| identification of a heroin esterase in rhodococcus sp. strain h1. | a strain of a rhodococcus sp. (termed h1) capable of utilizing heroin as its sole carbon and energy source was isolated by selective enrichment. an inducible heroin esterase was partially purified and shown to catalyze the hydrolysis of both of the acetylester groups of heroin. the enzyme displays optimum activity at ph 8.5 and appears to be a trimer of identical subunits with an m(r) or 39,000 and a native m(r) of 120,000. | 1994 | 7986057 |
| brain abscess due to gordona terrae in an immunocompromised child: case report and review of infections caused by g. terrae. | a brain abscess complicated antineoplastic chemotherapy for a primary cerebral rhabdoid tumor in an immunocompromised boy. culture of purulent exudate obtained by surgical puncture of an intracranial hematoma yielded a gram-positive microorganism initially identified as a rhodococcus species by conventional biochemical analysis; however, the isolate was subsequently identified as gordona terrae by ribosomal dna analysis. to our knowledge, this is the third case of human infection caused by g. te ... | 1994 | 7986897 |
| rhodococcus equi pneumonia in an hiv-infected patient. | we report a case of a 46-year-old hiv-infected patient suffering rhodococcus equi pneumonia and septicaemia. after the failure of an initial antibiotic treatment, the upper lobe of the right lung was resected due to a cavitating pneumonia. after that the patient stabilized for a period of 7 months by administration of a resistogram-adapted combination of tetracycline, erythromycin and clindamycin. due to the patient's decision, antibiotic treatment was stopped when a cytomegalovirus retinitis wa ... | 1994 | 7987063 |
| review of 40 cases of lung abscesses in foals and adult horses. | a retrospective study of 40 horses with primary lung abscesses without pleuropneumonia showed that lung abscesses occurred predominantly in young horses, usually in foals aged < or = 6 months. hyperfibrinogenaemia was present in all but 2 horses, and other common clinical and haematological findings were hyperthermia, tachycardia, polypnoea, and neutrophilic leucocytosis. the most common bacterial species isolated from transtracheal aspirates were streptococcus zooepidemicus (20/34) and rhodococ ... | 1994 | 7988535 |
| haemolytic and phospholipase c (plc) activities of rhodococcus equi. | rhodococcus equi, an intracellular organism causing pneumonia and lung abscesses in foals, is generally thought to be non-haemolytic. in the present study, however, 13 of 14 representative isolates were found to be haemolytic when tested on agar media containing washed red blood cells rather than whole blood. red cells of rabbits, dogs, horses and man were more sensitive to lysis than were those of ruminants. two new enzymatic activities of the species were defined: a lecithinase and a phosphati ... | 1994 | 7989259 |
| evaluation of a monoclonal antibody-based colony blot test for rapid identification of virulent rhodococcus equi. | we recently generated a monoclonal antibody immunoglobulin g1 (mab 10g5), which can recognize 15- to 17-kda antigens, virulence-associated antigens of rhodococcus equi, and developed a colony blot enzyme-linked immunosorbent assay with mab 10g5 for the rapid identification of virulent r. equi. in this epidemiologic study, we evaluated the results of the colony blot test in the identification of virulent isolates of r. equi from feces of horses and soil and compared them with those from a convent ... | 1994 | 7999891 |
| permeabilization of mycolic-acid-containing actinomycetes for in situ hybridization with fluorescently labelled oligonucleotide probes. | the application of whole-cell hybridization using labelled oligonucleotide probes in microbial systematics and ecology is limited by difficulties in permeabilizing many gram-positive organisms. in this investigation paraformaldehyde treatment, acid methanolysis and acid hydrolysis were evaluated as a means of permeabilizing mycolic-acid-containing actinomycetes prior to hybridization with a fluorescently labelled oligonucleotide probe designed to bind to a conserved sequence of bacterial 16s rrn ... | 1994 | 8000549 |
| a kinetic study and application of a novel carbonyl reductase isolated from rhodococcus erythropolis. | the newly described carbonyl reductase from rhodococcus erythropolis (recr) accepts a broad range of substrates. based on the kinetic constants of a variety of methyl and ethyl ketones a hypothetical model of the substrate-binding site is proposed. whether a substrate of interest may be reduced by the recr can be predicted from this model together with the kinetic data. a study of initial velocities and product inhibition is presented, which shows that the kinetics of the recr follow a theorell- ... | 1994 | 8000863 |
| enzymatic asymmetric synthesis of alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols by arylalkyl acylamidases. | with the novel microbial enzyme, 'arylalkyl acylamidase', optically active alpha-methyl arylalkylamines and alpha-methyl arylalkylalcohols have been obtained through enantioselective hydrolysis of their racemic amides and esters. (s)-enantiomers of 1-methylbenzylamine, 1-methyl-3-phenylpropylamine and 1-methyl-3-phenylpropanol of high optical purity (> 94% e.e.) were synthesized with the cells of nocardia erythropolis iam 1440 or cellulomonas fimi aku 671. (r)-enantiomer of 1-methyl-3-phenylprop ... | 1994 | 8000864 |
| stereoselective epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile. | the chiral intermediate (3s,4r)-trans-3,4-dihydro-3,4-dihydroxy-2,2-dimethyl- 2h-1-benzopyran-6-carbonitrile [(+)-trans diol 3] was made by the stereoselective microbial epoxidation of 2,2-dimethyl-2h-1-benzopyran-6-carbonitrile 1. this compound is a potential intermediate for the total synthesis of potassium-channel openers. several microbial cultures were found which catalyzed the transformation of 1 to the corresponding (3s,4s)-epoxide 2 and (+)-trans diol 3. the two best cultures, corynebact ... | 1994 | 8000876 |
| rapid degradation of the triazinone herbicide metamitron by a rhodococcus sp. isolated from treated soil. | seven bacterial isolates which degraded the herbicide metamitron (3-methyl-4-amino-6-phenyl-1,2,4-triazin-5-one) were obtained from field-enhanced soil by liquid enrichment culture. all isolates appeared to be identical and a representative, 0246b, was identified as a rhodococcus sp. by cell wall and fatty acid analyses. this isolate degraded metamitron as the sole source of carbon within 24 h at 25 degrees c and this is the first report of a bacterium capable of growing with metamitron as the s ... | 1994 | 8002472 |
| [emergent pathologies: rhodococcus equi infection in the acquired immunodeficiency syndrome]. | rhodococcus equi is an intracellular facultative, gram-positive, partially acid-fast, cocco-bacillary microorganism of increasing importance as a pathogen in severely immunocompromised hiv-positive patients. rhodococcus equi may cause invasive pulmonary infection, bacteremia and disseminated infection. rhodococcus equi is easily cultured from blood or sputum on standard media, but is frequently regarded as a contaminant. delay in establishing a definite diagnosis may result in a poor outcome in ... | 1994 | 8003385 |
| molecular cloning and nucleotide sequence of the gene coding photosensitive nitrile hydratase. | 1994 | 8010666 | |
| fatal rhodococcus equi infection in an angora goat. | 1994 | 8011764 | |
| metabolism of styrene by rhodococcus rhodochrous ncimb 13259. | rhodococcus rhodochrous ncimb 13259 grows on styrene, toluene, ethylbenzene, and benzene as sole carbon sources. simultaneous induction tests with cells grown on styrene or toluene showed high rates of oxygen consumption with toluene cis-glycol and 3-methylcatechol, suggesting the involvement of a cis-glycol pathway. 3-vinylcatechol accumulated when intact cells were incubated with styrene in the presence of 3-fluorocatechol to inhibit catechol dioxygenase activity. experiments with 18o2 showed ... | 1994 | 8017910 |
| purification and characterization of a 14-kilodalton protein that is bound to the surface of polyhydroxyalkanoic acid granules in rhodococcus ruber. | the n-terminal amino acid sequence of the polyhydroxyalkanoic acid (pha) granule-associated m(r)-15,500 protein of rhodococcus ruber (the ga14 protein) was analyzed. the sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated m(r) of 14,175 which was recently localized downstream of the pha synthase gene (u. pieper and a. steinbüchel, fems microbiol. lett. 96:73-80, 1992). a recombinant strain of escherichia coli xl1-b ... | 1994 | 8021220 |
| rhodococcus equi: first case in a heart transplant recipient. | rhodococcus equi is a gram-positive diphtheroid that occasionally affects immunocompromised patients, usually causing a chronic respiratory infection with cavitating pulmonary opacities on chest radiograph that resemble mycobacterial or fungal disease. etiologic diagnosis presents a number of pitfalls, because rhodococcus equi isolates mimic many of the characteristics of other microorganisms more familiar to the laboratory staff. the treatment of choice for this disease has not yet been establi ... | 1994 | 8031817 |
| a highly unusual combination of pulmonary pathogens in an hiv infected patient. | we report on a case with an unusual combination of pulmonary pathogens including pneumocystis carinii, rhodococcus equi and toxoplasma gondii in an hiv infected patient. the multiple microbiological procedures required to ascertain the etiological diagnoses of pneumonitis in hiv-seropositive subjects are discussed. | 1994 | 8036478 |
| [pulmonary malacoplakia and rhodococcus equi pneumonia in a patient infected with the human immunodeficiency virus. a case report with review of the literature]. | we report a case of pulmonary malakoplakia in a patient suffering from aids secondary to a rhodococcus equi pneumonia. the association between these two pathologies only occurring in the immunodepressed does not seen fortuitous. deficiency in cellular immunity and macrophage cellular activity as well as failure of intracellular bactericidal and phagolysosomal function are very probably the links. the treatment of this opportunistic germ rests on prolonged poly-antibiotic therapy or indeed surgic ... | 1994 | 8041997 |
| cloning and sequence analysis of the gene encoding isocitrate lyase from rhodococcus fascians. | an isocitrate lyase (icl)-encoding gene (icl) from the gram+ plant pathogen rhodococcus fascians was identified serendipitously as part of a scrambled fragment after shotgun cloning in the promoter probe vector, pdp1. the icl protein is 429 amino acids long (47.11 kda) and has a predicted pi of 4.84; it is 54% similar to the escherichia coli icl and 24-27% to eukaryotic homologues. comparison of the prokaryotic and eukaryotic icl confirms the earlier proposal of matsuoka and mcfadden [j. bacteri ... | 1994 | 8045408 |
| animal-associated opportunistic infections among persons infected with the human immunodeficiency virus. | a number of animal-associated infections occur in persons infected with the human immunodeficiency virus (hiv), including those due to toxoplasma gondii, cryptosporidium, microsporida, salmonella, campylo-bacter, giardia, rhodococcus equi, rochalimaea, and listeria monocytogenes. most of these infections, with the exception of those due to rochalimaea, appear to be acquired by the immunosuppressed individual from sources other than exposure to animals. drs. glaser and colleagues review our curre ... | 1994 | 8054433 |
| rhodococcus equi infection: a cause of cavitary pulmonary disease in immuno-compromised patients. | rhodococcus equi is a facultatively-aerobic, gram-positive and intracellular bacteria that causes pulmonary and disseminated infections in immuno-compromised patients. it is a common agent that causes pneumonia in farm animals and only rarely is transmitted to man. i report a case of pneumonia caused by this agent in a patient with aids who had been misdiagnosed as tuberculosis and treated as such for five months before the correct diagnosis was made. the diagnosis should be suspected on clinica ... | 1993 | 8055560 |
| [pneumonia caused by rhodococcus equi and salmonella spp. in a patient with human immunodeficiency virus infection]. | 1994 | 8059038 | |
| [rhodococcus equi infection in aids: a case with pulmonary abscess. review of the literature]. | rhodococcus equi is an easily missed opportunistic infection in patient infected by the human immunodeficiency virus. we report one case with lung abscess in a patient with aids with literature review. | 1994 | 8059147 |
| [pneumonia by rhodococcus equi in patients with hiv infection]. | we present the case of a patient with aids who developed abscessified pneumonia by rhodococcus equi. we publish this case given the rare frequency of presentation of this opportunistic infection in this type of patient and given its good clinical evolution, on the contrary of other cases described so far in the literature. the good evolution of the patient may be due to an early diagnosis, less than one month, and the combined medical therapy with rifampicine and tetracyclines. | 1994 | 8061140 |
| transfer of polychlorophenol-degrading rhodococcus chlorophenolicus (apajalahti et al. 1986) to the genus mycobacterium as mycobacterium chlorophenolicum comb. nov. | three independently isolated polychlorophenol-degrading strains of bacteria were characterized on the basis of chemotaxonomic and nutritional characteristics. previously, these strains were assigned to the species rhodococcus chlorophenolicus, which was described on the basis of the properties of one of the strains, strain pcp-i(t) (t = type strain) (j. h. a. apajalahti, p. kärpänoja, and m. s. salkinoja-salonen, int. j. syst. bacteriol 36:246-251, 1986). however, the results of analyses of myco ... | 1994 | 8068542 |
| sequence of rhodococcus gene cluster encoding the subunits of ethanolamine ammonia-lyase and an apc-like permease. | sequence analysis of a 5173-bp genomic fragment from the nocardioform actinomycete rhodococcus sp. strain ni86/21 revealed the presence of two genes, eutb and eutc, encoding the putative homologues of the large and small subunits of the ethanolamine ammonia-lyase, respectively, from salmonella typhimurium. this is the first report of the characterization of these genes in a gram-positive species. immediately upstream of eutb, a gene encoding a putative permease of the apc (amino acids, polyamine ... | 1994 | 8069783 |
| haloalkane degradation and assimilation by rhodococcus rhodochrous ncimb 13064. | the bacterium rhodococcus rhodochrous ncimb 13064, isolated from an industrial site, could use a wide range of 1-haloalkanes as sole carbon source but apparently utilized several different mechanisms simultaneously for assimilation of substrate. catabolism of 1-chlorobutane occurred mainly by attack at the c-1 atom by a hydrolytic dehalogenase with the formation of butanol which was metabolized via butyric acid. the detection of small amounts of gamma-butyrolactone in the medium suggested that s ... | 1994 | 8081504 |
| transthoracic needle aspiration in the study of pulmonary infections in patients with hiv. | to evaluate the safety and efficacy of transthoracic aspiration with an ultrathin needle in the microbiologic diagnosis of pulmonary infections in hiv-infected patients. | 1994 | 8082342 |
| rhodococcus equi pneumonia and occult hiv infection. | 1993 | 8102451 | |
| failure of pulmonary clearance of rhodococcus equi infection in cd4+ t-lymphocyte-deficient transgenic mice. | pulmonary clearance of rhodococcus equi requires functional t lymphocytes. in this study, cd8+ t-lymphocyte-deficient transgenic mice cleared virulent r. equi from the lungs while infection in cd4+ t-lymphocyte-deficient transgenic mice persisted. although both cd4+ and cd8+ t cells function early in pulmonary defense against r. equi, clearance is dependent on cd4+ t lymphocytes. | 1993 | 8104903 |
| pulmonary malakoplakia related to rhodococcus equi occurring in the acquired immunodeficiency syndrome. | 1994 | 8107640 | |
| lack of residual lung damage in horses in which rhodococcus equi-induced pneumonia had been diagnosed. | the effect of prior rhodococcus equi-induced pneumonia on pulmonary health was investigated in 5 horses (< 24 months old) using endoscopy, radiography, hematologic and bronchoalveolar lavage analyses, and pulmonary function testing. rhodococcus equi-induced pneumonia had been diagnosed in principal horses when they were foals. diagnosis was based on positive results of transtracheal aspiration and thoracic radiography at the time of initial clinical examination. results of reevaluation of the re ... | 1993 | 8116948 |
| rhodococcus zopfii sp. nov., a toxicant-degrading bacterium. | a toluene-degrading bacterial strain isolated from bioreactors was identified as a member of the genus rhodococcus on the basis of the following characteristics: meso-diaminopimelic acid, arabinose, galactose, and glucose are the diagnostic cellular sugars; the mycolic acids contain 33 to 36 carbon atoms; and the formation of a branching mycelium is followed by marked fragmentation of the mycelium into irregular rod-shaped to coccoid units. dna-dna hybridization analyses performed with type stra ... | 1994 | 8123552 |
| identification of dna involved in rhodococcus chromosomal conjugation and self-incompatibility. | genes essential for rhodococcus chromosomal conjugation were found to be located on an unstable genetic element in one of the strains investigated. from this element two segments of dna could be identified which were involved in conjugation of rhodococcus strains. one region, spread over 8 kb, was involved in the property of self-incompatibility. a second region, of about 3 kb, was essential for conjugation between atcc 12674 and related nocardioforms. | 1994 | 8125246 |
| analysis of three 2,3-dihydroxybiphenyl 1,2-dioxygenases found in rhodococcus globerulus p6. identification of a new family of extradiol dioxygenases. | the polychlorobiphenyl-degrading bacterium rhodococcus globerulus p6 contains three bphc genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenases. one of them, bphc1, is clustered with the bphb gene which encodes 2,3-dihydroxy-4-phenylhexa-4,6-diene dehydrogenase and constitutes part of the bph operon specifying the degradation of biphenyl. the nucleotide sequence of bphb and the three bphc genes has been determined. the protein products of the bphbc1 gene cluster were found to exhibit significant ... | 1994 | 8126007 |
| structural elucidation of the specific capsular polysaccharide of rhodococcus equi serotype 7. | structural analysis of the specific capsular polysaccharide produced by rhodococcus equi serotype 7 indicated it to be a high-molecular-weight polymer consisting of equal molar amounts of d-galactose, d-mannose, l-rhamnose, and pyruvic acid. by employing a combination of chemical and nmr techniques, it was established that the polysaccharide is composed of the linear repeating trisaccharide units: [formula: see text] -->3)-alpha-d-galp-(1-->3)-alpha-d-manp-(1-->3)-alpha-l-rhap -(1-->, in which t ... | 1994 | 8137362 |
| spectroscopic observation of the intramolecular electron transfer in the photoactivation processes of nitrile hydratase. | the photoactivation phenomena of the photosensitive enzyme nitrile hydratase (nhase) was studied by various spectroscopic methods. we have already shown that the photoactivation of nhase accompanies oxidation of an iron atom in the nhase [honda et al. (1992) febs lett. 301, 177-180]. from the results obtained in the present study by absorption, action, and fluorescence spectra, we show that the chromophore responsible for the photoactivation process is the iron complex, and the tryptophan residu ... | 1994 | 8142355 |
| rhodococcus equi vertebral osteomyelitis in foals. | 1994 | 8143655 | |
| rhodococcus equi vertebral osteomyelitis in 3 quarter horse colts. | 1994 | 8143671 | |
| vertebral body osteomyelitis due to rhodococcus equi in two arabian foals. | 1994 | 8143672 | |
| endophthalmitis in auckland 1983-1991. | a review was made of 58 cases of suspected endophthalmitis presenting to auckland public hospital between january 1983 and july 1991 (103 months). thirty culture positive cases were found comprising 13 early onset postoperative, six delayed onset postoperative, five bleb-associated, four post-traumatic and two endogenous cases. the most frequent organism grown in postoperative endophthalmitis was staphylococcus epidermidis. no conclusion could be reached as to the effectiveness of early versus d ... | 1993 | 8148139 |
| correlation between antibiotic resistance, phage-like particle presence, and virulence in rhodococcus equi human isolates. | rhodococcus equi is a gram-positive coccobacillus that appears to be emerging as a pulmonary pathogen in aids patients. in four human clinical isolates, two antibiotic resistance phenotypes were found to coexist: one beta-lactam resistant and the other beta-lactam susceptible. in vitro, beta-lactam-resistant mutants were obtained at a frequency of 1 x 10(-5) to 5 x 10(-5) from beta-lactam-susceptible strains on cephalothin-containing plates. neither beta-lactamase nor plasmid dna was detected in ... | 1994 | 8150945 |
| virulence of rhodococcus equi isolates from patients with and without aids. | rhodococcus equi is an emerging opportunistic pathogen of human immunodeficiency virus-infected patients. thirty-nine isolates of r. equi from immunocompromised patients with and without aids were analyzed for the presence of virulence plasmid dna, expression of 15- to 17-kda antigens, and their pathogenicities in mice. of the human isolates, eight contained an 85-kb virulence plasmid, expressed 15- to 17-kda antigens, and were virulent in mice. nineteen isolates carried cryptic plasmids of vari ... | 1994 | 8150957 |
| [slowly developing cavitated pneumonia in a patient with hiv infection]. | 1994 | 8155754 | |
| identification and characterization of a transmissible linear plasmid from rhodococcus erythropolis bd2 that encodes isopropylbenzene and trichloroethene catabolism. | rhodococcus erythropolis bd2, which is able to utilize isopropylbenzene as a sole carbon and energy source, was shown to contain a conjugative linear plasmid, pbd2. the estimated size of pbd2 is 208 to 212 kb. linear plasmid-deficient strains had lost both the isopropylbenzene degradation and trichloroethene degradation characteristics, as well as the arsenite resistance and mercury resistance phenotypes. reintroduction of pbd2 restored all four characteristics. conjugational transfer of pbd2 to ... | 1994 | 8161179 |
| the fas operon of rhodococcus fascians encodes new genes required for efficient fasciation of host plants. | three virulence loci (fas, att, and hyp) of rhodococcus fascians d188 have been identified on a 200-kb conjugative linear plasmid (pfid188). the fas locus was delimited to a 6.5-kb dna fragment by insertion mutagenesis, single homologous disruptive recombination, and in trans complementation of different avirulent insertion mutants. the locus is arranged as a large operon containing six open reading frames whose expression is specifically induced during the interaction with host plants. one pred ... | 1994 | 8169198 |
| humoral immune response to rhodococcus equi in aids patients with r. equi pneumonia. | 1994 | 8169421 | |
| the catechol 2,3-dioxygenase gene of rhodococcus rhodochrous ctm: nucleotide sequence, comparison with isofunctional dioxygenases and evidence for an active-site histidine. | in cell-free extracts of escherichia coli clones harbouring the 3.5 kb bg/ii fragment of plasmid ptc1 from rhodococcus rhodochrous ctm a catechol 2,3-dioxygenase (c23o) accepting both 3-methylcatechol and 2,3-dihydroxybiphenyl as substrates could be detected. the plasmid-encoded gene for c23o of r. rhodochrous ctm and its flanking regions were sequenced. in front of the gene a sequence resembling an e. coli promoter was identified, which led to constitutive expression of the cloned gene in e. co ... | 1994 | 8180697 |
| biotransformations catalyzed by the genus rhodococcus. | rhodococci display a diverse range of metabolic capabilities and they are a ubiquitous feature of many environments. they are able to degrade short-chain, long-chain, and halogenated hydrocarbons, and numerous aromatic compounds, including halogenated and other substituted aromatics, heteroaromatics, hydroaromatics, and polycyclic aromatic hydrocarbons. they possess a wide variety of pathways for degrading and modifying aromatic compounds, including dioxygenase and monooxygenase ring attack, and ... | 1994 | 8187203 |
| rhodococcus equi infections of humans. 12 cases and a review of the literature. | increased recognition of rhodococcus equi as a human pathogen has occurred since 1983, when the first review article summarized the world's literature of 12 cases. in this article, we present 12 cases from the university of oklahoma health sciences center and review 60 from the literature. most cases occur in immunocompromised hosts and present as chronic cavitary pneumonias. associated extrapulmonary disease is seen at diagnosis in 7% of patients with pneumonia, and relapse occurs at extrapulmo ... | 1994 | 8190035 |
| rapid identification of mycolic acid patterns of mycobacteria by high-performance liquid chromatography using pattern recognition software and a mycobacterium library. | current methods for identifying mycobacteria by high-performance liquid chromatography (hplc) require a visual assessment of the generated chromatographic data, which often involves time-consuming hand calculations and the use of flow charts. our laboratory has developed a personal computer-based file containing patterns of mycolic acids detected in 45 species of mycobacterium, including both slowly and rapidly growing species, as well as tsukamurella paurometabolum and members of the genera cor ... | 1994 | 8195387 |
| comparison of oral erythromycin formulations in the horse using pharmacokinetic profiles. | the pharmacokinetic properties of four erythromycin formulations were compared in six adult horses after administration of single and multiple oral doses. formulations of erythromycin administered were estolate and phosphate given 37.5 mg/kg every 12 h and 25 mg/kg every 8 h, and stearate and ethylsuccinate given 25 mg/kg every 8 h. areas under the curve (auc) and maximum plasma erythromycin concentrations (cmax) were equal or greater (p > or = 0.05) following administration of erythromycin phos ... | 1994 | 8196089 |
| sequences of the cobalamin biosynthetic genes cobk, cobl and cobm from rhodococcus sp. ni86/21. | sequence analysis of a 5753-bp genomic fragment of rhodococcus sp. ni86/21 revealed the presence of three homologues of known cobalamin biosynthetic genes. one of these genes encodes a protein showing strong homology with the precorrin-6x reductase (cobk) of pseudomonas denitrificans. in addition, the rhodococcal homologue of the corrin methyltransferases, cobm from p. denitrificans and cbif from salmonella typhimurium, was identified. the protein deduced from a third rhodococcus gene aligns wel ... | 1994 | 8200543 |
| effect of growth temperature on maintenance of virulent rhodococcus equi. | repeated passage of virulent rhodococcus equi atcc 33701 and l1 at 38 degrees c resulted in attenuation of the strains as a result of curing the virulence plasmid; at 30 degrees c, repeated passage had no such effect. at a temperature of 38 degrees c the plasmid-bearing cells replicated more slowly than their plasmid-cured derivatives and so were gradually replaced by cells lacking plasmids. in contrast, at a temperature of 30 degrees c the growth rate of either strain was not affected by the pr ... | 1994 | 8203124 |