Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of a membrane-regulated sugar phosphate phosphohydrolase from lactobacillus casei. | one of the key components of the futile xylitol cycle of lactobacillus casei cl-16 is a phosphatase which dephosphorylates xylitol 5-phosphate to xylitol prior to the expulsion of the pentitol from cells. this enzyme has been partially purified and characterized. the phosphatase is active against a variety of four-, five-, and six-carbon sugars and sugar alcohols phosphorylated at the terminal 4, 5, and 6 positions, respectively, but exhibits little or no affinity for substrates phosphorylated a ... | 1985 | 2993253 |
| isl1: a new transposable element in lactobacillus casei. | the genome structures of a temperate lactobacillus phage, phi fsw, and its virulent mutants, phi fsvs, were examined by restriction, heteroduplex and nucleotide-sequence analyses. the results showed that two out of three phi fsvs had the same 1.3 kbp insertion (designated as isl1) at different positions in the phi fsw sequence. isl1 was 1,256 bp long and contained at least two long open reading frames of 279 and 822 bases on one strand. inverted repeats were found at the termini of the isl1 whic ... | 1985 | 2993817 |
| dihydrofolate reductase. 1h resonance assignments and coenzyme-induced conformational changes. | lactobacillus casei dihydrofolate reductase has been studied in solution by one and two-dimensional 1h nuclear magnetic resonance (n.m.r.) spectroscopy at 500 mhz. by using a combination of n.m.r. methods in conjunction with the crystal structure of the enzyme-methotrexate-nadph complex, resonances have been assigned for 32 of the 162 residues of the enzyme. these are widely distributed throughout the structure of the protein, and include all the histidine and tyrosine residues, as well as sever ... | 1986 | 3012098 |
| protection of mice against herpes simplex virus infection by a lactobacillus casei preparation (lc 9018) in combination with inactivated viral antigen. | heat-killed lactobacillus casei yit 9018 (lc 9018) cells enhanced the resistance to herpes simplex virus type 1 (hsv-1) in adult mice, but not significantly. the protection of mice against hsv-1 infection and the production of neutralizing antibodies were significantly enhanced by the administration of lc 9018 in combination with inactivated hsv-1 antigen. the optimal enhancement of resistance was seen in mice 14 days after the simultaneous administration of these substances. the resistance to h ... | 1986 | 3012292 |
| in vitro binding of potent mutagenic pyrolysates to intestinal bacteria. | the ability of 22 strains of intestinal bacteria to bind the mutagenic pyrolyzates--3-amino-1,4-dimethyl-5h-pyrido-[4,3-b]indole [(trp-p-1) cas: 62450-06-0], 3-amino-1-methyl-5h-pyrido [4,3-b]indole [(trp-p-2) cas: 62450-07-1], 2-amino-6-methyldipyrido[1,2-a:3',2'-d]imidazole [(glu-p-1) cas: 67730-11-4], 2-aminodipyrido[1,2-a:3',2'-d]imidazole [(glu-p-2) cas: 67730-10-3], 2-amino-3-methylimidazo[4,5-f]quinoline [(iq) cas: 76180-96-6], 2-amino-3,4-dimethylimidazo[4,5-f]quinoline [(meiq) cas: 7709 ... | 1986 | 3014197 |
| acid tolerance, proton permeabilities, and membrane atpases of oral streptococci. | differences in acid tolerance among representative oral streptococci were found to be related more closely to the dynamic permeabilities of the bacteria to protons than to differences in the sensitivities of cell membranes to gross damage caused by environmental acidification. for streptococcus mutans gs-5, streptococcus sanguis nctc 10904, and streptococcus salivarius atcc 13419, gross membrane damage, indicated by the release of magnesium from whole cells, occurred at ph values below about 4 a ... | 1986 | 3015800 |
| the role of superoxide anion and lysosomal enzymes in anti-listerial activity of elicited peritoneal macrophages. | the in vitro effect of superoxide anion and lysosomal enzyme activity on the killing of listeria monocytogenes egd (listeria) by peritoneal macrophages (pm) was investigated. generation of superoxide anion by pm stimulated with phorbol myristate acetate (pma) was significantly increased by intraperitoneal injection of lactobacillus casei yit 9018 (lc9018) or corynebacterium parvum (cp), but not by injection of peptone. however, superoxide anion generation by lc9018-elicited pm stimulated with li ... | 1986 | 3020680 |
| [phosphoenolpyruvate:sugar phosphotransferase systems in a strain of lactobacillus casei subsp. casei]. | 1986 | 3022022 | |
| spin probes as mechanistic inhibitors and active site probes of thymidylate synthetase. | c-4- and c-5-substituted analogues of dump were examined as inhibitors of thymidylate synthetase and as topographical probes of its active site by electron spin resonance (esr). the c-5-substituted spin-labeled analogues pduap (2) and a pdutt (3) as well as the unlabeled aadump (1) were competitive inhibitors with ki's of 9.2, 89, and 7.9 microm, respectively. the c-4-spin-labeled pls4du (4) displayed no inhibition activity. scatchard plots as determined by esr gave similar association constants ... | 1986 | 3027327 |
| 5-quinone derivatives of 2'-deoxyuridine 5'-phosphate: inhibition and inactivation of thymidylate synthase, antitumor cell, and antiviral studies. | both photochemical aromatic substitution and palladium (0)-catalyzed biaryl coupling reactions have been employed in the synthesis of 5-substituted 2'-deoxyuridines. the former procedure was useful in the preparation of the 3,4-dimethyl-2,5-dimethoxyphenyl derivative 12a and the 3,4,6-trimethyl-2,5-dimethoxyphenyl derivative 12b. the latter reaction was efficient in the preparation of the 2-(3-methyl-1,4-dimethoxynaphthyl) derivative 14. these compounds and their nucleotides (20a-c) were convert ... | 1987 | 3027341 |
| molecular characterization of the cell cycle-regulated thymidylate synthase gene of saccharomyces cerevisiae. | the complete nucleotide sequence of a 1.8-kilobase dna fragment containing the cell cycle-regulated thymidylate synthase gene (tmp 1) of the yeast saccharomyces cerevisiae is presented. this analysis has revealed a 912-base pair open reading frame which encodes a 304-amino acid residue protein with a calculated mr of 35,007. the tmp1-6 and cdc21-1 mutant alleles of this gene also have been sequenced, and both show single base pair changes which would result in different amino acid substitutions. ... | 1987 | 3031048 |
| overview: rational basis for development of fluoropyrimidine/5-formyltetrahydrofolate combination chemotherapy. | fluorodeoxyuridylate (fdump) and thymidylate synthase (ts) are one of the better understood systems of drug-target interaction in cancer chemotherapy. isolation and characterization of ts (initially from lactobacillus casei and later from a variety of other sources), cloning and sequencing of the gene, determination of the 3-d structure of the enzyme by x-ray diffraction, and elucidation of the structure of both the catalytic intermediate and the enzyme-inhibitor complex have revealed critical p ... | 1988 | 3073650 |
| identification of the 1h resonances of valine and leucine residues in dihydrofolate reductase by using a combination of selective deuteration and two-dimensional correlation spectroscopy. | lactobacillus casei dihydrofolate reductase (mr 18 500) contains 16 valine and 14 leucine residues. by comparing the 2d cosy nmr spectra of normal and [gamma-2h6]valine enzyme we have been able to identify all 60 methyl resonances from these residues, and to connect the pairs arising from the same residue. this pairing of the methyl resonances was aided by the examination of the 2d relay spectrum which also allowed the c alpha h resonances (and hence the complete spin systems) of 14 of the valin ... | 1986 | 3079708 |
| stopped-flow studies of 2'-deoxynucleotide binding to thymidylate synthase. | the mechanism of 2'-deoxynucleotide binding to lactobacillus casei thymidylate synthase was studied using stopped-flow kinetic techniques to monitor the decrease in intrinsic protein fluorescence upon complex formation. the data were consistent with a two-step mechanism involving a rapid preequilibrium step to form the enzyme-2'-deoxynucleotide complex followed by a slow isomerization step. rate and equilibrium constants were determined for the three 2'-deoxynucleotides (2'-deoxyuridylate, 2'-de ... | 1986 | 3082285 |
| effect of human lysozyme on 2-deoxyglucose uptake by streptococcus mutans and other oral microorganisms. | 1986 | 3082516 | |
| crystallographic investigation of the cooperative interaction between trimethoprim, reduced cofactor and dihydrofolate reductase. | the structure of the complex between e. coli (rt500) form i dihydrofolate reductase, the antibacterial trimethoprim and nadph has been determined by x-ray crystallography. the inhibitor and cofactor are in mutual contact. a flexible chain segment which includes met 20 is in contact with the inhibitor in the presence of nadph, but more distant in its absence. by contrast, the inhibitor conformation is little changed with nadph present. we discuss these observations with regard to the mutually coo ... | 1986 | 3082676 |
| inactivation of thymidylate synthase by chlorine disinfectants. | the effects of two chlorine disinfectants, calcium hypochlorite (hth) and 3-chloro-4,4-dimethyl-2-oxazolidinone (agent i), on the activity of thymidylate synthase have been investigated. although both disinfectants inactivated the enzyme, the following differences were observed: when the two disinfectants were used at the same total chlorine concentration, the rate and extent of inactivation were greater with agent i than hth. the substrate dump partially protected thymidylate synthase from inac ... | 1986 | 3082691 |
| enhancement of hematopoietic response of mice by subcutaneous administration of lactobacillus casei. | mice that had received heat-killed lactobacillus casei (lc 9018) subcutaneously (s.c.) showed enhanced resistance to systemic (i.e., intravenous) infection with listeria monocytogenes, but the antilisterial resistance of mice was less augmented by s.c. administration of propionibacterium acnes ("corynebacterium parvum"). though there was little change in the total number of splenic leukocytes after s.c. administration of lc 9018, the monocyte-macrophage ratio increased after treatment, reaching ... | 1986 | 3082755 |
| forms of human milk folacin and variation patterns. | the pattern of folacin in 180 human milk samples collected from 16 women was studied before and after pteroylglutamic hydroxylase (conjugase) treatment. the differential response of lactobacillus casei versus streptococcus faecalis distinguishes n-5-methyltetrahydrofolate from other forms. growth of pediococcus cerevisiae differentiates the reduced monoglutamates, other than the n-5-methylated derivative, from oxidized folacins. mean folacin activity with l. casei was 85.3 ng/ml. prior to conjug ... | 1986 | 3083081 |
| lactobacillus endocarditis. | two cases of endocarditis due to lactobacillus plantarum and lactobacillus casei var. rhamnosus are described. both patients were successfully treated medically. the response of the two isolates to a range of concentrations of penicillin in an apparatus which continuously monitored growth is reported. the strain of l. casei var. rhamnosus was found to be penicillin tolerant. the use of a combination of a penicillin with gentamicin to treat endocarditis due to penicillin tolerant strains of lacto ... | 1986 | 3084661 |
| membrane-associated folate transport proteins. | 1986 | 3084910 | |
| high-performance liquid chromatographic separation of the naturally occurring folic acid derivatives. | 1986 | 3084911 | |
| bacterial folylpoly(gamma-glutamate) synthase-dihydrofolate synthase. | 1986 | 3084914 | |
| trimethoprim binding to lactobacillus casei dihydrofolate reductase: a 13c nmr study using selectively 13c-enriched trimethoprim. | we have measured the 13c chemical shifts for trimethoprim molecules selectively enriched with 13c at the 2-, 4-, 5-, 6-, and 7-positions and the p-och3 position in their complexes with lactobacillus casei dihydrofolate reductase in the presence and absence of coenzyme analogues. the c2 carbon shifts indicate that the pyrimidine ring is protonated at n1 in all the complexes of trimethoprim with the enzyme and coenzymes and in each case the pyrimidine ring is binding in a similar way to that of th ... | 1986 | 3085709 |
| calcium-dependent salivary agglutinin with reactivity to various oral bacterial species. | human parotid agglutinins from three individuals were isolated by adsorption to and desorption from strains of streptococcus mutans belonging to serotypes a, b, c, d, and e and strains of lactobacillus casei, actinomyces viscosus, and streptococcus sanguis. the desorption was achieved by suspending centrifuged saliva-coated microorganisms in 10 mm phosphate buffer (ph 6.8) containing 0.154 m sodium chloride. after another centrifugation, agglutinin activity was recovered in the supernatants. the ... | 1986 | 3087878 |
| effects of lactobacillus casei on pseudomonas aeruginosa infection in normal and dexamethasone-treated mice. | a single intraperitoneal injection of lactobacillus casei yit 0003 into normal or dexamethasone-treated mice led to nonspecific resistance against intraperitoneal challenge with lethal doses of pseudomonas aeruginosa pao 3047. the enhanced resistance was retained for 14 days (p less than 0.05) after injection with living l. casei. in contrast, the statistically significant duration of the enhanced resistance in mice treated intraperitoneally with living l. acidophilus yit 0075 was only 5 days. t ... | 1986 | 3088397 |
| microbiological analysis of 5-formyltetrahydrofolic acid and other folates using an automatic 96-well plate reader. | the growth of auxotrophic bacteria remains the method of choice for the determination of biologically active folate metabolites in plasma. this report describes a microbiological assay for folates adapted to use disposable 96-well plates and an automatic plate reader. the modifications in the assay decreased reagent costs and made the analysis of hundreds of samples per day possible with a sensitivity limit of 10 fmol of (6s)-5-formyltetrahydrofolic acid. this limit compares favorably with that ... | 1986 | 3089059 |
| preparation of (6r)-tetrahydrofolic acid and (6r)-5-formyltetrahydrofolic acid of high stereochemical purity. | commercially available 5-formyltetrahydrofolate (5-cho-h4pteglu) is chemically prepared in a reaction that introduces an asymmetric center at the 6 carbon, and hence is the mixture of diastereomers differing in chirality about this position. (6r)-5-cho-h4pteglu, the diastereomer that is not normally found in vivo, was prepared from folic acid. folic acid was chemically reduced and (6r)-tetrahydrofolate (h4pteglu) was obtained from the resultant (6r,s)-h4pteglu by enzymatic consumption of the nat ... | 1986 | 3089060 |
| [effect of antibiotics on the adhesive properties of microorganisms and an available model for its study]. | the effect of benzylpenicillin, ampicillin, bicillin-3, carbenicillin, levomycetin, erythromycin, streptomycin and kanamycin on the adhesive properties of 9 test microbes including 5 strains of lactobacillus from human microflora, 3 uropathornic strains of e. coli and 1 strain of s. aureus was studied with the method developed by the authors. the method is based on the use of formalinized human erythrocytes as macroorganism cells. it was shown that the antibiotic inhibitory effect on adhesion de ... | 1986 | 3089137 |
| trapping and partial characterization of an adduct postulated to be the covalent catalytic ternary complex of thymidylate synthase. | the proposed mechanism of action of thymidylate synthase envisages the formation of a covalent ternary complex of the enzyme with the substrate dump and the cofactor 5,10-methylenetetrahydrofolate (ch2h4folate). the proposed structure of this adduct has been based by analogy on that of the covalent inhibitory ternary complex thymidylate synthase-fdump-ch2h4folate. our recent success in using the protein precipitant trichloroacetic acid to trap the latter complex and covalent binary complexes of ... | 1986 | 3089276 |
| antitumor activity of lactobacillus casei (lc 9018) against experimental mouse bladder tumor (mbt-2). | the purpose of this paper is to investigate the inhibitory effect of lactobacillus casei (lc 9018) on the growth of transplantable murine bladder tumor. lactobacillus casei, viable and/or heat-killed bacteria, either in oral or intravenous administration, produced a significant inhibitory effect on the tumor growth and much less pulmonary metastasis. | 1986 | 3090281 |
| effects of lactobacillus casei-containing ointment on the healing and protection against opportunistic infection of thermal injury wounds in mice. | 1986 | 3091538 | |
| induction by lactobacillus casei of increase in macrophage colony-forming cells and serum colony-stimulating activity in mice. | the content of macrophage colony-forming cells (m-cfc) and the serum colony-stimulating activity (csa) were investigated in mice after intravenous administration of lactobacillus casei yit9018 (lc9018). in normal balb/c mice, 500 micrograms of lc9018 increased both femoral and splenic m-cfc; the highest levels were found a few days and a week, respectively, after the administration. lc9018 also induced an increase in splenic m-cfc in c3h/hej mice as well as in c3h/hen mice, unlike lipopolysaccha ... | 1986 | 3091557 |
| folate analogues. 26. syntheses and antifolate activity of 10-substituted derivatives of 5,8-dideazafolic acid and of the poly-gamma-glutamyl metabolites of n10-propargyl-5,8-dideazafolic acid (pddf). | the poly-gamma-glutamyl derivatives of n10-propargyl-5,8-dideazafolic acid (pddf) with a chain length of up to five glutamate residues were synthesized from n10-propargyl-5,8-dideazapteroic acid by the solid-phase procedure. these compounds were evaluated for their antifolate activity using folate-requiring microorganisms and intact and permeabilized l1210 cells and as inhibitors of dihydrofolate reductase and thymidylate synthase derived from l. casei. the polyglutamylated derivatives of pddf ( ... | 1986 | 3091832 |
| synthesis and antifolate properties of 5,10-methylenetetrahydro-8,10-dideazaminopterin. | the synthesis of the 5,10-methylene analogue of 5,6,7,8-tetrahydro-8,10-dideazaminopterin, a potential dual inhibitor of dihydrofolate reductase (dhfr) and thymidylate synthase (ts) enzymes, is described. the dimethyl ester of 10-carboxy-4-amino-4-deoxy-8,10-dideazapteroic acid was converted to the tetrahydro derivative by hydrogenation. thermally induced cyclization of the 10-carbomethoxy and the 5-nh groups afforded the 5,10-carbonyl analogue. reduction of the lactam with borane readily yielde ... | 1986 | 3091834 |
| [standardization of the direct hemagglutination reaction induced by bacteria of the genus lactobacillus]. | l. bulgaricus, l. casei var. rhamnosus, and l. plantarum cells grown in agar-containing (but not liquid) thioglycol medium for 3 days have been shown to be capable of inducing mannose-resistant agglutination of human and guinea-pig erythrocytes, while giving a considerably weaker reaction with erythrocytes from animals of other species (ox, sheep, dog, rabbit, rat and mouse). the optimum conditions for the test are presented. | 1986 | 3094303 |
| effects of lactobacillus casei on hematology and blood chemistry in normal and burned mice. | 1986 | 3095273 | |
| computerized semiautomated microbiological assay of folacin. | a semiautomated microbiological folacin assay system is described. a microcomputer controls sample dilutions, medium addition, turbidity determination, and data acquisition. assay capacity is 600 tubes per day, approximately twice that of comparable manual assays. using the automated equipment, more samples can be compared within one assay, eliminating many sources of between-assay variation in large studies. additional advantages of this system are reduced human errors, flexibility of assay des ... | 1986 | 3095308 |
| the effect of lowering the ph on the composition and metabolism of a community of nine oral bacteria grown in a chemostat. | nine oral bacteria, associated with both healthy and diseased sites in the mouth, were grown at d = 0.05 h-1 (mean generation time 13.9 h) in a glucose-limited chemostat. after an initial period of steady-state growth at ph 7.0, ph control was discontinued. the ph then decreased until it stabilized at ph 4.1 after 9 d (16 generations), while the eh rose from -165 mv to +160 mv. the lowering in ph resulted in the composition and metabolism of the flora being altered and in increased bacterial agg ... | 1986 | 3095488 |
| radiation resistance of lactobacilli isolated from radurized meat relative to growth and environment. | of 113 lactobacilli isolated from radurized (5 kgy) minced meat, 7 lactobacillus sake strains, 1 l. curvatus strain, and 1 l. farciminis strain were used for radiation resistance studies in a semisynthetic substrate (i.e., modified mrs broth). five reference lactobacillus spp., one staphylococcus aureus strain, and one salmonella typhimurium strain were used for comparative purposes. all l. sake isolates exhibited the phenomenon of being more resistant to gamma-irradiation in the exponential (lo ... | 1986 | 3096207 |
| clinical manifestations and therapy of lactobacillus endocarditis: report of a case and review of the literature. | a case of lactobacillus casei endocarditis that occurred on a carpentier-edwards porcine valve is reported. a review of the literature, which yielded 23 other reports of endocarditis due to this organism, suggests that lactobacillus is a rare cause of endocarditis. typically, it occurs in a patient with preexisting structural heart disease (20 of 24 [83%]) and often with some form of recent dental infection or manipulation (18 of 24 [75%]). six (25%) of 24 patients died of this infection; howeve ... | 1986 | 3097786 |
| conformational analysis of thymidylate synthase from amino acid sequence and circular dichroism. | circular dichroism studies were carried out in the vacuum ultraviolet region for thymidylate synthase from lactobacillus casei and its ligand complexes. the cd spectrum was analyzed for secondary structure by our method and the variable selection method, and both gave similar results. our method predicts 33% alpha-helix, 25% (23% antiparallel and 2% parallel) beta-sheet, 20% turns, and 16% other structure. the secondary structure of this protein was also predicted from the amino acid sequence by ... | 1986 | 3098289 |
| crystallization and crystallographic data for new forms of thymidylate synthase from lactobacillus casei. | several new crystal forms of thymidylate synthase (5,10-methlenetetrahydrofolate:dump c-methyltransferase; ec 2.1.1.45) were obtained by controlled ph change. in the crystals the dimeric molecule has a 2-fold symmetry axis coinciding with crystallographic symmetry. the crystals scatter to at least 2.7 a resolution in the synchrotron x-ray beam and appear to be suitable for high-resolution x-ray diffraction analysis. the crystals were successfully derivatized and preliminary results are reported ... | 1986 | 3098984 |
| atomic structure of thymidylate synthase: target for rational drug design. | the atomic structure of thymidylate synthase from lactobacillus casei was determined at 3 angstrom resolution. the native enzyme is a dimer of identical subunits. the dimer interface is formed by an unusual association between five-stranded beta sheets present in each monomer. comparison of known sequences with the lactobacillus casei structure suggests that they all have a common core structure around which loops are inserted or deleted in different sequences. residues from both subunits contri ... | 1987 | 3099389 |
| folate analogues. 25. synthesis and biological evaluation of n10-propargylfolic acid and its reduced derivatives. | n10-propargylfolic acid (2), which is the closest pteridine analogue of the thymidylate synthase inhibitor n10-propargyl-5,8-dideazafolic acid (pddf), was synthesized starting from diethyl [p-(n-propargylamino)benzoyl]-l-glutamate (5) and n-(3-bromo-2-oxopropyl)phthalimide (8). the 7,8-dihydro derivative of propargylfolic acid served as a synthetic substrate of lactobacillus casei dihydrofolate reductase. propargylfolic acid and its reduced derivatives were weak inhibitors of l. casei thymidylat ... | 1986 | 3100797 |
| in vitro and in vivo release of cytostatic factors from lactobacillus casei-elicited peritoneal macrophages after stimulation with tumor cells and immunostimulants. | the effect of tumor cells and immunostimulants on the release of cytostatic factors (cf) from lactobacillus casei yit 9018 (lc)-, corynebacterium parvum (cp)- or peptone-elicited peritoneal macrophages (pm) was investigated in vitro and in vivo. significant release of cf into the culture medium from pm elicited with lc was induced by seven of eight mitomycin c-pretreated tumor cell lines and not by normal spleen cells, while no cf was released extracellularly from peptone-elicited pm given the s ... | 1987 | 3102062 |
| electron microscopic study of the process of dna ejection from the head of pl-1, a lactobacillus casei phage. | the process of dna ejection from the head of pl-1, a lactobacillus casei atcc 27092 phage, was studied by electron microscopy by counting the number of ghost particles with empty heads among phages already adsorbed to the cell. the process of dna ejection was temperature- and live cell-dependent. | 1987 | 3102685 |
| antimetastatic effect of lactobacillus casei yit9018 (lc 9018) on a highly metastatic variant of b16 melanoma in c57bl/6j mice. | the effect of lactobacillus casei yit9018 (lc 9018) on a highly metastatic variant of b16 melanoma, b16-bl6, was determined in c57bl/6 mice. intralesional (i.l.) injection of lc 9018 inhibited tumor growth and prolonged the survival after s.c. inoculation of b16-bl6 into c57bl/6 mice. injection of lc 9018 i.v. protected the mice against pulmonary metastasis after i.v. inoculation of b16-bl6. injection of lc 9018 i.l. before surgical excision of the primary tumor inhibited axillary lymph node met ... | 1987 | 3103914 |
| purification and characterization of ribitol-5-phosphate and xylitol-5-phosphate dehydrogenases from strains of lactobacillus casei. | a simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. the former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol. the xylitol-5-phosphate dehydrogenase exists as a tetrameric protein with a molecular weight of 180,000; this enzyme oxidizes the phosphate esters of both xylitol and d-arabitol. c ... | 1987 | 3104310 |
| assembly of d-alanyl-lipoteichoic acid in lactobacillus casei: mutants deficient in the d-alanyl ester content of this amphiphile. | d-alanyl-lipoteichoic acid (d-alanyl-lta) from lactobacillus casei atcc 7469 contains a poly(glycerophosphate) moiety that is acylated with d-alanyl ester residues. the physiological function of these residues is not well understood. five mutant strains of this organism that are deficient in the esters of this amphiphile were isolated and characterized. when compared with the parent, strains an-1 and an-4 incorporated less than 10% of d-[14c]alanine into lta, whereas an-2, an-3, and an-5 incorpo ... | 1987 | 3104312 |
| antibody for detection and quantitation of membrane-associated folate-binding protein from lactobacillus casei. | lactobacillus casei cells contain a 25 kda, membrane-associated, folate-binding protein (fbp), which is a component of the folate transport system. polyclonal antibody to fbp (anti-fbp) has been prepared, and conditions have been established for detection and quantitation of the protein. anti-fbp did not block [3h]folate transport or binding in l. casei cells. as judged by western blots, the antibody reacted only with fbp on sodium dodecyl sulfate electrophoretograms of triton x-100 extracts of ... | 1987 | 3107428 |
| high performance liquid chromatographic determination of riboflavin in food--a comparison with a microbiological method. | the riboflavin content of commercial products of different origin was determined by reversed phase liquid chromatography. the method was compared with a standard microbiological assay with lactobacillus casei. a comparison between the two methods gave a correlation coefficient of 0.9996 for vitamin enriched foods, dairy and health-food products. for these products the lc-method is well adapted for use in routine work. the results obtained from unenriched flours and flours with a high rate of ext ... | 1987 | 3108182 |
| purification and characterization of dihydrofolate reductase from soybean seedlings. | dihydrofolate reductase (dhfr; ec 1.5.1.3) was purified to homogeneity from soybean seedlings by affinity chromatography on methotrexate-aminohexyl sepharose, gel filtration on ultrogel aca-54, and blue sepharose chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme gave a single protein band corresponding to a molecular weight of 22,000. the enzyme is not a 140,000 da heteropolymer as reported by others. amino acid sequence-specific antibodies to intact human d ... | 1987 | 3109322 |
| nuclear magnetic resonance spectra of lipoteichoic acid. | lipoteichoic acid acids with a range of chemical compositions have been studied using 1h; 13c- and 31p-nuclear magnetic resonance. proton spectroscopy provided a rapid method for demonstrating whether alanine in a sample is covalently bound to the polyglycerophosphate chains and for monitoring hydrolysis of alanine. the nature of sugar substituents can be determined, with some limitations, from the 13c spectra, and the proportions of glycerol residues substituted by alanine and sugar can be meas ... | 1987 | 3109478 |
| 15n and 1h nmr evidence for multiple conformations of the complex of dihydrofolate reductase with its substrate, folate. | the binding of folate to lactobacillus casei dihydrofolate reductase in the presence and absence of nadp+ has been studied by 15n nmr, using [5-15n]folate. in the presence of nadp+, three separate signals were observed for the single 15n atom, in agreement with our earlier evidence from 1h and 13c nmr for multiple conformations of this complex [(1982) biochemistry 21, 5831-5838]. the 15n spectra of the binary enzyme-folate complex provide evidence for the first time that this complex also exists ... | 1987 | 3109940 |
| [morphological changes in rabbit pleura induced by lactobacillus casei (lc9018)]. | 1987 | 3111385 | |
| antimicrobial action of new, proprietary lining cements. | the antibacterial activity of innovative, commercial lining cements was investigated. a liner which contains calcium hydroxide and is polymerized by visible light (prisma vlc dycal) and a glass-ionomer lining cement (gc lining cement) were compared with two more established lining cements (advanced formula ii dycal and life). antibacterial activity and hemolysis-like agar change at 24, 48, and 72 hours were measured on blood agar plates inoculated with streptococcus mutans kpsk 2 (serotype c), l ... | 1987 | 3112201 |
| proteolytic activity of crude cell-free extract of lactobacillus casei and lactobacillus plantarum. | crude cell-free extracts of some strains of each l. casei and l. plantarum were assayed for their amino-, imino- and endopeptidase as well as the caseinolytic activity. l-alanine-, l-phenylalanine- and l-leucine-p-nitroanilide but not l-glutamic acid-p-nitroanilide, were hydrolyzed by all the strains indicating an amino-peptidase activity. the activity of proline iminopeptidase was very low compared to that of the aminopeptidase. l. casei could be distinguished from l. plantarum by its high endo ... | 1987 | 3112575 |
| [studies on the synthesis of dihydrofolate reductase inhibitor, 5-(substituted phenyl)-thio(seleno)-2, 4-diaminopyrimidines and their inhibitory activity on l. casei and chicken liver dhfr]. | 1987 | 3113176 | |
| lysozyme-protease-inorganic monovalent anion lysis of oral bacterial strains in buffers and stimulated whole saliva. | similar to streptococcus mutans, buffer suspensions of lactobacillus casei, lactobacillus plantarum, and fusobacterium nucleatum all undergo cell lysis when treated with the lysozyme-protease-inorganic monovalent anion antibacterial system. for lactobacillus species treated with lysozyme and proteases at phs of 4 and 5.3, lysis resulted when a lytic activating concentration of bicarbonate anion followed enzyme treatment. furthermore, synergistic lysis of these bacteria was noted when lysozyme-pr ... | 1987 | 3114343 |
| role of colony-stimulating activity in antitumor activity of lactobacillus casei in mice. | wide-ranging differences were observed between the antitumor activities of 23 lactobacilli (13 species; 23 strains) and their capacities to elevate the level of serum colony-stimulating activity (csa) by intraperitoneal administration in mice, and a good correlation existed between the two activities. the mechanism of enhanced production of csa by administration of lactobacillus casei yit 9018 (lc 9018), one of the bacteria that had the strongest activities, and the role of csa in antitumor acti ... | 1987 | 3114399 |
| peptidases of lactobacillus casei and l. plantarum. | fractionation of cell-free extracts of lactobacillus casei ncdo 151 (grown in man's et al. (1960) broth) by polyacrylamide disc electrophoresis showed the presence of 4 constitutive peptidases. the enzymes appear to be a tripeptidase with a narrow substrate specificity, two true dipeptidases with identical broad-specificity, and a probable carboxypeptidase with broad-specificity. a probable amino peptidase could also be isolated from the cell-free extract by density gradient electrofocusing. gro ... | 1987 | 3114469 |
| the role of humoral immunity in lactobacillus casei cell wall induced arthritis. | although both cellular and humoral immunity have been shown to play important roles in the development of collagen induced arthritis, their roles in the pathogenesis of bacterial cell wall induced arthritis remain unclear. previous studies of humoral immunity in bacterial cell wall induced arthritis were carried out in outbred animals and measured the humoral response to only the eliciting bacteria. we compared the humoral immune responses of arthritis susceptible lewis and arthritis resistant f ... | 1987 | 3114482 |
| [immunopotentiating activity of lactic bacteria administered by oral route. favorable effect in infantile diarrheas]. | 1986 | 3114592 | |
| factors affecting the rate of breakdown of bacterial protein in rumen fluid. | 1. the cellular proteins of butyrivibrio fibrisolvens, lactobacillus casei, megasphaera elsdenii, selenomonas ruminantium and streptococcus bovis were labelled by growth in the presence of l-[14c]leucine, and the breakdown of labelled protein was measured in incubations of these bacteria with rumen fluid to which unlabelled 5 mm-l-leucine was added. the rate of protein breakdown was estimated from the rate of release of radioactivity into acid-soluble material. 2. protein breakdown occurred at d ... | 1987 | 3118940 |
| dihydrofolate reductase: specificity and conformational change. | 1987 | 3119395 | |
| synthesis and biological evaluation of 8-deazahomofolic acid and its tetrahydro derivative. | the syntheses of 8-deazahomofolic acid and its tetrahydro derivative, potential inhibitors of thymidylate synthase (ts) and other folate related enzymes, are described. wittig condensation of 2-acetamido-6-formyl-4-pyrimidinol with the triphenylphosphine ylide 3 derived from n-acetyl-4-(p-carbethoxyanilino)-1-chloro-2-butanone, hydrogenation of the enone intermediate 5, introduction of a 5-amino group via diazonium coupling, and reductive ring closure yielded ethyl n11-acetyl-8-deazahomopteroate ... | 1988 | 3121855 |
| preparation and regeneration of bacteriophage pl-1 enzyme-induced lactobacillus casei protoplasts. | lactobacillus casei atcc 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the pl-1 phage lysates of this bacterium, at 37 degrees c for 1.5 h in 50 mm tris hydrochloride buffer (ph 7.2) containing 20% sucrose and 10 mm mgso4. the protoplasts lacked the cell wall layer but retained the ability to transport l-[3h]glutamine. the frequency of regeneration was about 1%, whereas about 99% of the cells were osmotically sensitive. | 1987 | 3122657 |
| direct experimental evidence for competitive inhibition of dihydrofolate reductase by methotrexate. | steady-state enzyme kinetic techniques at very low enzyme concentration (0.4 nm) were used successfully to measure the inhibition constant (53 pm) for the dissociation of methotrexate from the ternary complex of methotrexate, nadph and dihydrofolate reductase from lactobacillus casei; and to demonstrate unequivocally that the inhibition was, indeed, competitive with respect to dihydrofolate. | 1988 | 3122764 |
| thermodynamic characterization of the interactions of methotrexate with dihydrofolate reductase by quantitative affinity chromatography. | affinity chromatography on methotrexate-sepharose has been used to evaluate dissociation constants for interactions of methotrexate with dihydrofolate reductase from lactobacillus casei. equilibrium constants of 0.25 microm and 0.6 nm were obtained for dissociation of the inhibitor from the enzyme-methotrexate and enzyme-nadph-methotrexate complexes, respectively, these estimates being in good agreement with the corresponding published values for dihydrofolate reductase from streptococcus faeciu ... | 1988 | 3122765 |
| systemic augmentation of the immune response in mice by feeding fermented milks with lactobacillus casei and lactobacillus acidophilus. | this study investigates the effect of feeding fermented milks with lactobacillus casei, lactobacillus acidophilus and a mixture of both micro-organisms on the specific and non-specific host defence mechanisms in swiss mice. animals fed with fermented milk for 8 days (100 micrograms/day) showed an increase in both phagocytic and lymphocytic activity. this activation of the immune system began on the 3rd day, reached a maximum on the 5th, and decreased slightly on the 8th day of feeding. in the 8- ... | 1988 | 3123370 |
| role of macrophages in serum colony-stimulating factor induction by lactobacillus casei in mice. | heat-killed lactobacillus casei yit9018 (lc9018), when injected intravenously into mice at a dose of 4 to 40 mg/kg, induced the production of serum colony-stimulating factor (csf). since this induction was observed in both c3h/hej and c3h/hen mice, lc9018 was considered to act differently from lipopolysaccharide. the amount of serum csf induced by lc9018 in nude mice and whole-body-x-ray-irradiated mice was similar to that in control mice, but the induction of serum csf was suppressed by the pre ... | 1988 | 3123388 |
| production of potent polyclonal antibodies to bacterial membrane amphiphiles. | lipid a (la), ketodeoxyoctonate (kdo) and lipoteichoic acids (lta) were used to produce homologous polyclonal antibodies. these haptens were administered to rabbits in differing immunogenic forms, using multiple intradermal and intraperitoneal injections with complete freund adjuvant. booster injections were either made intradermally with incomplete freund adjuvant or intravenously in saline. the immune-response was monitored regularly with an enzyme-immunoassay. lipid a and kdo covalently linke ... | 1987 | 3123902 |
| insights into enzyme function from studies on mutants of dihydrofolate reductase. | kinetic analysis and protein mutagenesis allow the importance of individual amino acids in ligand binding and catalysis to be assessed. a kinetic analysis has shown that the reaction catalyzed by dihydrofolate reductase is optimized with respect to product flux, which in turn is predetermined by the active-site hydrophobic surface. protein mutagenesis has revealed that specific hydrophobic residues contribute 2 to 5 kilocalories per mole to ligand binding and catalysis. the extent to which pertu ... | 1988 | 3125607 |
| structural comparisons of complexes of methotrexate analogues with lactobacillus casei dihydrofolate reductase by two-dimensional 1h nmr at 500 mhz. | we have used two-dimensional (2d) nmr methods to examine complexes of lactobacillus casei dihydrofolate reductase and methotrexate (mtx) analogues having structural modifications of the benzoyl ring [the 3',5'-difluoro and 3',5'-dichloro analogues (ii and iii)] and also the glutamic acid moiety [the alpha- and gamma-monoamides (iv and v)]. assignments of the 1h signals in the spectra of the various complexes were made by comparison of their 2d spectra with those of complexes containing methotrex ... | 1987 | 3126805 |
| kinetics and thermodynamics of the interaction of 5-fluoro-2'-deoxyuridylate with thymidylate synthase. | thymidylate synthase (ts), 5-fluorodeoxyuridylate (fdump), and 5,10-methylenetetrahydrofolate (ch2-h4folate) form a covalent complex in which a cys thiol of ts is attached to the 6-position of fdump and the one-carbon unit of the cofactor is attached to the 5-position. the kinetics of formation of this covalent complex have been determined at several temperatures by semirapid quench methods. together with previously reported data the results permit calculation of every rate and equilibrium const ... | 1987 | 3126806 |
| [antitumor activity of lactobacillus casei on experimental mouse bladder tumor (mbt-2)]. | 1987 | 3127622 | |
| a flexible multiple sequence alignment program. | the 'regions' method for multisequence alignment used in the previously reported program malign has been generalized to include recursive refinement so that unaligned portions between two regions at the current level of resolution can be handled with increased resolution. additionally, there is incorporated a limiting of the number of regions to be used at any level of resolution from which to abstract an alignment. this provides a significant increase in speed over the unlimited version. the pr ... | 1988 | 3127810 |
| correlation of susceptibility and cytostatic factor-inducing activity of tumour cells to peritoneal macrophages. the role of concanavalin a-binding glycopeptides extracted from the tumour cell surface. | l929, 3t12-3, b16, 3ll, and yac1 cells with cytostatic factor (cf)-inducing activity from lactobacillus casei-elicited murine peritoneal macrophages (lcepm) were susceptible to the cytostatic activity of lcepm and to lcepm-produced cf, but l1210, p388d1, and colon 26 cells, which have no cf-inducing activity, were resistant to that of lcepm and and to the cf. the resistance of p815 cells to that of lcepm was stronger than that of 3t12-3 cells, but the cf-inducing activity of p815 cells was about ... | 1988 | 3127880 |
| comparison of gamma-glutamyl hydrolase (conjugase; ec 3.4.22.12) and amylase treatment procedures in the microbiological assay for food folates. | 1. it has been suggested that deconjugation of food folates with pig kidney, compared with chicken pancreas, may increase measureable folate by approximately 50% (phillips & wright, 1983). therefore deconjugation with conjugases from these two enzyme sources was optimized and compared. folate was measured microbiologically with lactobacillus casei (atcc 7469) as the test organism at ph 5.6. 2. treatment for 6 h with 200 mg pig kidney/200 mg sample was compared with the conventional assay employi ... | 1988 | 3129012 |
| comparison of paired-ion liquid chromatographic method with aoac fluorometric and microbiological methods for riboflavin determination in selected foods. | a paired-ion liquid chromatographic (lc) technique coupled with fluorometric detection to determine riboflavin in various food matrices is described. chromatograms of many foods showed 2 peaks of interest due to presence of riboflavin and flavin mononucleotide (fmn). relatively high levels of fmn were found in raw beef, corned beef, chicken liver, and canned mushrooms. when riboflavin and fmn contents were summed, lc values were comparable to those obtained by the aoac standard procedures. the l ... | 1988 | 3129395 |
| nucleotide sequence of the beta-d-phosphogalactoside galactohydrolase gene of lactobacillus casei: comparison to analogous pbg genes of other gram-positive organisms. | lactose metabolism in lactobacillus casei occurs via phosphoenolpyruvate-dependent phosphotransferase uptake of lactose and subsequent cleavage of lactose-6-phosphate by beta-d-phosphogalactoside galactohydrolase (p-beta gal). the genes for lactose uptake and p-beta gal have been shown to be plasmid-associated in l. casei 64h [chassy et al., curr. microbiol. 1 (1978) 141-144]. the cloned p-beta gal-coding gene (pbg) previously described [lee et al., j. bacteriol. 152 (1982) 1138-1146] was subclo ... | 1988 | 3130295 |
| molecular cloning and nucleotide sequence of the factor iiilac gene of lactobacillus casei. | the lactose-specific factor iii (fiiilac of the phosphoenolpyruvate-dependent phosphotransferase system (pts) was isolated from lactobacillus casei and purified to homogeneity by conventional protein purification methods. its apparent native mr, estimated from steric exclusion chromatography (approx. 39 kda), and subunit mr, estimated from sodium dodecyl sulfate-polyacrylamide gels, indicated that it exists as a trimer of identical subunits of 13 kda. the gene for fiii l. casei lac was cloned in ... | 1988 | 3130296 |
| protective and therapeutic efficacy of lactobacillus casei against experimental murine infections due to mycobacterium fortuitum complex. | a bacterial immunopotentiator, lc 9018 (heat-killed lactobacillus casei), was studied for its protective and therapeutic efficacies against mycobacterium fortuitum and m. chelonae infections in mice. this agent reduced the incidence of spinning disease and gross renal lesions and enhanced the elimination of organisms at the site of infection in the host mice, when administered intramuscularly six times a week (0.1 mg dry weight per injection, one injection on each day of treatment) from 1 week b ... | 1987 | 3130460 |
| dynamics of trimethoprim bound to dihydrofolate reductase. | the conformation of a small molecule in its binding site on a protein is a major factor in the specificity of the interaction between them. in this paper, we report the use of 1h and 13c nmr spectroscopy to study the fluctuations in conformation of the anti-bacterial drug trimethoprim when it is bound to its "target," dihydrofolate reductase. 13c relaxation measurements reveal dihedral angle changes of +/- 25 degrees to +/- 35 degrees on the subnanosecond time scale, while 13c line-shape analysi ... | 1988 | 3131763 |
| endocarditis caused by lactobacillus casei subspecies rhamnosus. a case report. | a case of subacute endocarditis in a 66-year-old man with mitral incompetence is described. the causative agent was identified as lactobacillus casei subspecies rhamnosus, which is a normal commensal in the mouth. despite the ubiquitous presence of these mouth commensals, this type of endocarditis appears to be rare. it is emphasised that a lactobacillus species should be considered in cases of clinically obvious endocarditis with 'negative' blood cultures, including 'negative' subcultures follo ... | 1988 | 3131885 |
| antitumor effect of intrapleural administration of lactobacillus casei in mice. | the antitumor effect of intrapleural (i.pl.) administration of lactobacillus casei yit 9018 (lc 9018) on meth a sarcoma in balb/c mice was examined. inoculation of meth a cells into the thoracic cavity of balb/c mice caused growth of the cells and the mice died from the tumor with an increased amount of pleural fluid. lc 9018 was given i.pl. to balb/c mice before or after i.pl. inoculation of meth a cells and the survival of the mice was determined. the i.pl. administration of lc 9018 was effect ... | 1988 | 3133110 |
| correlation between increase in ia-bearing macrophages and induction of t cell-dependent antitumor activity by lactobacillus casei in mice. | when lactobacillus casei yit 9018 (lc 9018) or corynebacterium parvum, known to be immunomodulators possessing antitumor activity, were injected i.p. into balb/c mice, peritoneal exudate macrophage ia antigen detected by indirect immunofluorescence method was expressed on their cell surface, but it was not expressed following the injection of 10% proteose peptone, an inflammatory agent, or lactobacillus fermentum yit 0159 (lf 0159), which have no antitumor activity. the percentage and absolute n ... | 1988 | 3133111 |
| variable expression of lactobacillus casei cell wall-induced coronary arteritis: an animal model of kawasaki's disease in selected inbred mouse strains. | mice receiving a single intraperitoneal injection of lactobacillus casei cell wall fragments in aqueous suspension develop an asymmetric inflammatory coronary arteritis which histologically mimics the lesions seen in the coronary arteritis of children with kawasaki's disease. a large variety of mice with genetically determined defects of the immune system were evaluated in this study to determine the influence of these defects on disease expression. only the c3h/hej mouse with defective macropha ... | 1988 | 3133145 |
| vancomycin resistance of clinical isolates of lactobacilli. | strains of lactobacilli were cultured from two patients with clinical evidence of infection. each isolate was found to be highly resistant to vancomycin but inactivation of the antibiotic could not be demonstrated. lactobacillus isolates should be regarded as potential pathogens. reliable vancomycin susceptibility testing is required since susceptibility among gram-positive bacteria in general should not be assumed. | 1988 | 3135337 |
| vitamin contents of archaebacteria. | the levels of six water-soluble vitamins of seven archaebacterial species were determined and compared with the levels found in a eubacterium, escherichia coli. biotin, riboflavin, pantothenic acid, nicotinic acid, pyridoxine, and lipoic acid contents of halobacterium volcanii, methanobacterium thermoautotrophicum delta h, "archaeoglobus fulgidus" vc-16, thermococcus celer, pyrodictium occultum, thermoproteus tenax, and sulfolobus solfataricus were measured by using bioassays. the archaebacteria ... | 1988 | 3137215 |
| intravaginal instillation of lactobacilli for prevention of recurrent urinary tract infections. | urinary tract infections remain a common problem, particularly in the female population. new methods are required to manage recurrent cystitis, and extensive research to date has suggested that restoration of the lactobacilli flora of the urogenital tract may prevent these infections. in this study, five females suffering from recurrent urinary tract infections were treated twice weekly with intravaginal and perineal implantation of lactobacillus casei gr-1. these organisms colonized the epithel ... | 1988 | 3138016 |
| lactobacillus inhibitor production against escherichia coli and coaggregation ability with uropathogens. | previous investigations have shown that certain strains of lactobacilli can competitively exclude uropathogens from attaching to uroepithelial cells and from causing urinary tract infection in animals. the finding of an inhibitory effect produced by lactobacillus casei ssp. rhamnosus gr-1 against the growth of uropathogens was investigated further using two escherichia coli indicator strains hu 734 and atcc 25922. there were two phases to the inhibitor studies. the first one using an agar sandwi ... | 1988 | 3138017 |
| [changes in the function of macrophages after thermal injury and effect of lactobacillus casei on the function of macrophages]. | 1988 | 3138329 | |
| bacteriophages of lactobacilli. | lactobacilli are members of the bacterial flora of lactic starter cultures used to generate lactic acid fermentation in a number of animal or plant products used as human or animals foods. they can be affected by phage outbreaks, which can result in faulty and depreciated products. two groups of phages specific of lactobacillus casei have been thoroughly studied. 1. the first group is represented by phage pl-1. this phage behaves as lytic in its usual host l. casei atcc 27092, but can lysogenize ... | 1988 | 3139059 |
| cloning and expression of the phospho-beta-galactosidase genes on the lactose plasmid and the chromosome of lactobacillus casei c257 in escherichia coli. | a 4.7 kb bglii fragment of lactose plasmid ply101 of lactobacillus casei c257, which hybridized weakly to a fragment including a streptococcal phospho-beta-galactosidase (p-beta-gal) gene, was cloned into plasmid vectors of escherichia coli. transformant cells expressed p-beta-gal activity, confirming the presence of a p-beta-gal gene on ply101. deletion mapping showed that the structural gene of p-beta-gal was located on the 2.4 kb pvui-ecorv fragment. the direction of transcription was determi ... | 1988 | 3139071 |
| analysis and characterization of the folates in the nonmethanogenic archaebacteria. | a detailed analysis of the folate coenzymes in the nonmethanogenic archaebacteria has been performed. by using the lactobacillus casei microbiological assay for folates, the levels of folates in sulfolobus solfataricus and sulfolobus acidocaldarius were found to be 3.7 and 8.3 ng/g (dry weight) of cells, respectively, compared with 88,000 and 28,000 ng/g (dry weight) of cells in halobacterium halobium and halobacterium strain gn-1, respectively. the levels of folates found in the sulfolobus spp. ... | 1988 | 3139633 |
| the role of lymph node cells in the inhibition of metastasis by subcutaneous injection of lactobacillus casei in mice. | the role of lymph node cells in the inhibition of metastasis of b16-bl6, a highly metastatic variant of b16 melanoma, by lactobacillus casei yit9018 (lc 9018) in c57bl/6 mice was determined. subcutaneous (s.c.) injection of lc 9018 into the left inguinal or the left axillary region inhibited both axillary lymph node and lung metastasis of b16-bl6 after surgical excision of the tumors resulting from inoculation into the left front footpad of the mice. there was, however, little inhibition of the ... | 1988 | 3139973 |
| animal models of vasculitis. lessons we can learn to improve our understanding of kawasaki disease. | kawasaki disease is an inflammatory illness of unknown etiology which is often complicated by coronary arteritis. systematic study of the pathologically similar coronary arteritis induced in mice by a single intraperitoneal injection of lactobacillus casei cell wall fragments may provide useful clues to the pathogenesis and appropriate treatment of kawasaki disease. studies of mouse strains with inbred immune deficiencies suggest that the coronary artery lesions may result from macrophage-mediat ... | 1988 | 3140306 |
| lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates. | microbiological assay is still widely used for estimating folic acid derivatives in serum and other biological samples. we describe here a modification of this procedure involving use of 96-well microtiter plates. this procedure, used with modern, computer-interfaced microtiter-plate readers and data-reduction software, greatly shortens the time and minimizes reagent costs for this assay. under the conditions of our assay procedures, all folic acid derivatives tested gave equal growth response f ... | 1988 | 3141087 |