Publications

effects of idebenone on memory impairment induced in ischemic and embolization models of cerebrovascular disturbance in rats.	two rat models of memory impairment in passive avoidance learning induced by cerebrovascular disturbance, were established to estimate the effects of a cerebral metabolic enhancer, idebenone. transient and global cerebral ischemia in rats, produced by 4-vessel occlusion for 200 s immediately after the acquisition trial of passive avoidance learning, shortened the latencies in the retention test trial performed 24 h later. this retrograde amnesia was reversed significantly by idebenone administer ...	1989	2764639
morphologic and morphometric studies on tumor necrosis produced by radiotherapy, and hyperthermia singly and in combination.	the purpose of the present study was to evaluate the therapeutic effects of hyperthermia associated with radiotherapy on neoplasia. two transplanted experimental tumors (undifferentiated carcinoma of mouse breast and sarcoma 37) were used. a protocol was followed that included, for both models, four groups of animals: 1) control group; 2) radiotherapy group; 3) hyperthermia group; 4) radiotherapy associated with hyperthermia group. the animals were sacrificed after therapy according to a differe ...	1989	2770309
training elements at different levels in the strategies for control of schistosomiasis.	recently acquired comprehensive knowledge concerning the epidemiology of schistosomiasis has provided the background for the establishment of schistosomiasis control strategies. however, the planning, implementation, and maintenance of such control programmes requires sufficient numbers of well trained personnel at the local, district and central levels. training of health personnel as well as motivation of the community are fundamental and essential elements in any schistosomiasis control progr ...	2009	2772521
[chronic pyelonephritis in polycystic kidney].	the characteristics of chronic pyelonephritis are studied in 37 patients out of a total of 53 patients with proved renal polycystosis. a group of 71 patients with chronic pyelonephritis selected at random are used as a control group. the frequency of chronic pyelonephritis among the patients with renal polycystosis is 69.8%. the difference between the mean age of the patients with renal polycystosis and chronic pyelonephritis and the patients with renal polycystosis without chronic pyelonephriti ...	1989	2773465
cerebral embolization in two young patients with fascioscapulohumeral muscular dystrophy and atrial dysrhythmias.		2009	2773783
human aldehyde dehydrogenase: coenzyme binding studies.	the binding of nadh and nad+ to the human liver cytoplasmic, e1, and mitochondrial, e2, isozymes at ph 7.0 and 25 degrees c was studied by the nadh fluorescence enhancement technique, the sedimentation technique, and steady-state kinetics. the binding of radiolabeled [14c]nadh and [14c]nad+ to the e1 isozyme when measured by the sedimentation technique yielded linear scatchard plots with a dissociation constant of 17.6 microm for nadh and 21.4 microm for nad+ and a stoichiometry of ca. two coenz ...	1989	2775711
opening remarks of president fernando e. agrait on the occasion of the 50th anniversary of the cayo santiago macaque colony.		1989	2780954
the induction of protective immunity to leishmania major in the balb/c mouse by interleukin 4 treatment.	the ability of interleukin 4 (il4), administered subcutaneously around the cutaneous lesion in the form of hydrophilic gels, to affect the development of established leishmania major infections in the balb/c mouse was studied. il4 had a therapeutic effect on l. major lesion growth compared with control mice, causing not only resolution of the parasite lesions over a period of 10 weeks but also rendering animals resistant to reinfection. adoptive transfer of splenic t cells, obtained from il4-tre ...	1989	2786473
cyclosporin a inhibits activation-induced cell death in t-cell hybridomas and thymocytes.	one mechanism by which the immune system develops the ability to discriminate self from nonself is the deletion of autoreactive t-cell clones during thymic maturation. the drug cyclosporin a (csa) has been shown to interfere with this process, allowing the escape of normally 'forbidden' t-cell clones and the appearance of autoimmune disease. recently, it has been demonstrated that immature thymocytes undergo programmed cell death (apoptosis) upon activation via the t-cell receptor. a similar phe ...	2009	2786609
elevation of factor vii activity and mass in young adults at risk of ischemic heart disease.	the northwick park heart study found that elevation of factor vii in middle-aged subjects was an independent risk factor for subsequent ischemic heart disease. the present study was designed to determine whether factor vii elevation is present at a younger age and whether zymogen or activated factor vii is responsible for this elevation. a group of 20 asymptomatic first degree relatives (mean age 34.9 years) of patients with premature ischemic heart disease were compared with 15 age-matched norm ...	2009	2794283
[the polymerase chain reaction].		2010	2797295
the development of analytical models for human trypanosomiasis.	the idea of biological equilibria is presented and applied first to the case of single species populations. difference and differential descriptions of population changes over time are compared and contrasted. the complications that arise when describing interactions between species are then illustrated for the case of vector-borne diseases, such as malaria. a simple model for malaria is explored and later extended to the case of the african trypanosomiases where more than one vertebrate host sp ...	1989	2802828
steroidogenesis in liposomal system containing adrenal microsomal cytochrome p-450 electron transfer components.	purified adrenal microsomal p-450c21 and/or p-45017 alpha,lyase were incorporated with purified nadph-cytochrome-p-450 reductase into liposome membranes composed of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine at a molar ratio of 5:3:1. the rate dependences of reduction of liposomal p-450c21 in the fast phase as well as progesterone hydroxylation activities of p-450c21 and p-45017 alpha,lyase on the reductase concentration in the liposome membranes suggested that electron ...	1989	2804111
clinical experience with percutaneous intra-aortic balloon pumping in cardiogenic shock complicating acute myocardial infarction.	from july 1984 to december 1987, intra-aortic balloon pumping (iabp) was attempted percutaneously to 22 patients at the ages of 37-78 with cardiogenic shock or medically refractory heart failure complicating acute myocardial infarction (cs/mrhf-ami). there was only one failure because of severe bilateral iliofemoral atherosclerosis. of the 21 patients undergoing iabp, 17 were in cardiogenic shock and 13 of them showed reversal of shock syndrome after 10 to 48 hours of iabp. cardiac index increas ...	2009	2804784
clinical experience with percutaneous intra-aortic balloon pumping in cardiogenic shock complicating acute myocardial infarction.	from july 1984 to december 1987, intra-aortic balloon pumping (iabp) was attempted percutaneously to 22 patients at the ages of 37-78 with cardiogenic shock or medically refractory heart failure complicating acute myocardial infarction (cs/mrhf-ami). there was only one failure because of severe bilateral iliofemoral atherosclerosis. of the 21 patients undergoing iabp, 17 were in cardiogenic shock and 13 of them showed reversal of shock syndrome after 10 to 48 hours of iabp. cardiac index increas ...	2009	2804784
[intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy].	the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ...	2009	2812257
[intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy].	the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ...	2009	2812257
modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats.	the state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (ki) for the circulating tracer [14c]alpha-aminoisobutyric acid. pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral gluc ...	1989	2812289
modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats.	the state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (ki) for the circulating tracer [14c]alpha-aminoisobutyric acid. pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral gluc ...	1989	2812289
pharmacokinetics of postoperative intravenous indomethacin in children.	the disposition of indomethacin was studied in children aged one to four years. indomethacin 0.35 mg/kg was administered as an intravenous infusion during 15 min. venous blood samples were collected until 24 hr after infusion. serum indomethacin was determined with gas chromatography. using a non-linear regression analysis, the individual data were fitted by a two-compartment open mammillary model with central elimination. calculated pharmacokinetic parameters were (mean +/- sd); alpha half-life ...	2010	2813287
[functional paraganglioma (pheochromocytoma) in an aortico-pulmonary location].		2009	2815638
[functional paraganglioma (pheochromocytoma) in an aortico-pulmonary location].		2009	2815638
a vaccine for ovine cysticercosis.		1989	2815974
a rapid fluorometric method for semiautomated determination of cytotoxicity and cellular proliferation of human tumor cell lines in microculture.	a fluorometric method for the determination of cellular growth and cytotoxicity of human tumor cell lines in 96-well microculture plates is described. the assay is based on the combined use of the dna-binding dye hoechst 33342 and the fluorogenic substrate fluorescein diacetate (fda). hoechst 33342 undergoes a dramatic enhancement of fluorescence when specifically intercalated with cellular dna, whereas the fda fluorescence is dependent on cellular hydrolysis of the non-fluorescent substrate int ...	1989	2817793
leiomyosarcoma of uterus: a review of 14 cases.	a detailed clinicopathologic study of 14 patients with leiomyosarcoms of the uterus was made. twelve of 14 patients were followed up for 2 or more years. the median age was 49, the youngest patient was 20, and the oldest was 68. the predominant symptoms were abnormal vaginal bleeding and abdominal pain. the preoperative dilation and curettage established the correct diagnosis was 2 of 6 patients (33%). the predominant method of treatment was total abdominal hysterectomy (tah). five patients had ...	2009	2819573
leiomyosarcoma of uterus: a review of 14 cases.	a detailed clinicopathologic study of 14 patients with leiomyosarcoms of the uterus was made. twelve of 14 patients were followed up for 2 or more years. the median age was 49, the youngest patient was 20, and the oldest was 68. the predominant symptoms were abnormal vaginal bleeding and abdominal pain. the preoperative dilation and curettage established the correct diagnosis was 2 of 6 patients (33%). the predominant method of treatment was total abdominal hysterectomy (tah). five patients had ...	2009	2819573
in situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease.	we have developed an in situ hybridization assay capable of detecting enteroviral rna in myocardial cells, using molecularly cloned coxsackievirus b3 cdna as a diagnostic probe. because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group a and b coxsackieviruses and the echoviruses, detection of these various agents commonly implicated in human viral heart disease is possible in a single hybridization assay. we demonstrate the consid ...	1987	2819870
subcellular localization of enzymes in streptomyces aureofaciens and its alteration by benzyl thiocyanate. i. phosphatases and atp-glucokinase.	mycelia of a low- and a high-production strain of streptomyces aureofaciens were converted into protoplasts and divided into five subcellular fractions in order to localize exopolyphosphatases (ec 3.6.1.11), triphosphatase (ec 3.6.1.25), inorganic diphosphatase (ec 3.6.1.1), apyrase (ec 3.6.1.5) and glucokinase (ec 2.7.1.2). the highest specific activity of enzymes hydrolyzing polyphosphates was found in cytoplasmic vesicles and membranes. triphosphatase was detected in the periplasmic fraction. ...	1987	2826319
construction of an ileal reservoir in patients with a previous straight endorectal ileal pull-through.	a decade has passed since martin and associates presented the first series of endorectal ileal pull-through (pt) procedures with straight ileoanal anastomosis for treatment of ulcerative colitis and polyposis. during the ensuing years, several medical centers have reported their results using the pt. although some children have experienced good results, the majority of adults and children have had severe problems associated with high stool frequency, urgency, and incontinence. between 1977 and 1 ...	2009	2839124
construction of an ileal reservoir in patients with a previous straight endorectal ileal pull-through.	a decade has passed since martin and associates presented the first series of endorectal ileal pull-through (pt) procedures with straight ileoanal anastomosis for treatment of ulcerative colitis and polyposis. during the ensuing years, several medical centers have reported their results using the pt. although some children have experienced good results, the majority of adults and children have had severe problems associated with high stool frequency, urgency, and incontinence. between 1977 and 1 ...	2009	2839124
[experimental study on liver cancer--effect of intra-arterial injection of adriamycin-lipiodol emulsion on vx2 liver tumor].		1988	2846718
molecular biological characterization of equine herpesvirus type 1 (ehv-1) isolates from ruminant hosts.	isolates of equine herpesvirus type 1 (ehv-1) originating from affected antelope and cattle were compared with reference ehv-1 and ehv-4 isolates and were characterized. based on cross-neutralization, dna restriction profiles and blot-hybridization data these isolates could be characterized as ehv-1. one isolate (from an antelope) with a different restriction profile showed significant dna homology with ehv-1, partial homology with ehv-4, and little or no homology with ehv-2 and hsv-1 dnas. blot ...	1988	2849252
conversion from cyclosporin to azathioprine/prednisolone.		2010	2857436
the smoking gun.		2010	2857863
rhesus haemolytic disease treated with high-dose intravenous immunoglobulin.		2010	2860352
limitations of 131i-mibg scintigraphy in locating pheochromocytomas.	131i-metaiodobenzylguanidine (131i-mibg) scintigraphy for the location of pheochromocytomas has proved to be a major advance in patient management. in combination with computerized tomographic scanning, nearly all pheochromocytomas can be located before surgery and invasive investigations are now indicated only in exceptional cases. however, there are still lessons to be learned concerning the optimal administration and interpretation of 131i-mibg scintigraphy. with careful attention to detail a ...	2010	2861667
tromsø: lessons from a new curriculum.		2010	2863501
possible role of insulin deficiency and growth hormone in severe retinopathy.		2010	2863521
[drug information. sedatives and their application].		2010	2876530
[enterotoxemia in newborn calves due to cl. perfringens types a, c and d].	the clinical symptoms and the morphologic picture of calf enterotoxemia are described. studied were a total of thirty-two dead and slaughtered animals. bacteriologically, the disease was shown to be caused by types a, d, and c of clostridium perfringens. types c and d proved pathogenic for guinea pigs, while type a did not. isolated was a strain of clostridium perfringens, which had high toxigenicity. it was found that calves were fairly often affected with the disease. most severe were the infe ...	1986	2876542
intradermal vaccination against hepatitis b.		2007	2878212
a case of wound botulism.		2010	2882064
first-trimester prenatal diagnosis for huntington's disease with dna probes.	polymorphic dna probes linked to the locus for huntington disease (hd) were used for prenatal diagnosis of a 10-week fetus at 25% risk for the disease. the fetus proved to have a 48% risk of having inherited the hd mutation which was similar to that for the at-risk parent (50%). on this basis the parents elected to terminate the pregnancy. when appropriate family members are available and dna studies are informative, prenatal diagnosis of hd with polymorphic dna probes can determine the at-risk ...	2010	2884412
interactions between adenosine and alpha 1-adrenergic agonists in regulation of respiration in hamster brown adipocytes.	respiration in brown adipocytes can be increased by beta-adrenergic receptor agonists or by alpha 1-adrenergic receptor agonists (phenylephrine and norepinephrine). previous studies have shown that beta receptor-stimulated respiration is inhibited by adenosine and that enzymatic removal of adenosine produced by fat cells under normal incubation conditions enhances the respiratory response to beta receptor activation. the present experiments were performed to determine the effect of adenosine on ...	2010	2885736
double-blind comparison of loratadine (sch 29851), astemizole, and placebo in hay fever with special regard to onset of action.	in this double-blind study, 65 patients with hay fever were treated for 14 days with either loratadine 10 mg once daily, astemizole 10 mg once daily, or placebo. on the third day of treatment loratadine was statistically superior to placebo, but astemizole and placebo showed no significant differences according to the ratings for symptomatology, efficacy, and individual symptoms. adverse effects were minimal and did not differ between the three groups. loratadine had a significantly earlier onse ...	2010	2904776
diverticular disease of the appendix.	a retrospective study of diverticular disease of the appendix was made in 3,343 consecutive instances of appendectomies. a 2 per cent incidence of diverticular disease was found. these instances were classified into four morphologic types: 1, acute diverticulitis; 2, acute appendicitis with acute diverticulitis; 3, acute appendicitis with diverticulum, and 4, appendix with diverticulum. types 1, 2 and 3 were divided into subgroups with or without perforation. the elements of clinical behavior in ...	2009	2909128
alterations of coronary blood flow and reserve with aging in fischer 344 rats.	to determine whether aging affects the coronary circulation, left and right ventricular coronary blood flow and vascular resistance at rest and after maximal vasodilation were measured by left atrial injection of radioactive microspheres in conscious, unrestrained male fischer 344 rats at 4, 12, and 20 mo of age. as a function of age, maximal coronary blood flow per 100 g of tissue decreased by 43% in the left ventricle at both 12 and 20 mo, whereas a 44 and a 47% reduction was found in the righ ...	1989	2912199
effect of reduced energy metabolism and reperfusion on the permeability and morphology of the capillaries of an isolated rete mirabile.	the effects of reduction in energy metabolism were explored in the eel rete mirabile, an organ composed predominantly of capillaries. in vitro experiments showed that glycolysis is the major pathway of energy production in this capillary tissue, and that iodoacetate, kcn, and low po2 in combination markedly reduce its atp generation. when in situ energy generation was inhibited by this combination during countercurrent perfusion of the arterial and venous capillaries of the rete, an approximate ...	1989	2912596
duration of ischemia is vital for collateral development: repeated brief coronary artery occlusions in conscious dogs.	the effects of two types of repeated transient coronary artery occlusions on collateral development were examined in chronically instrumented, conscious dogs. a 2-minute coronary occlusion at 32-minute intervals (group 1, n = 11) or a 15-second occlusion at 4-minute intervals (group 2, n = 7) were repeated day and night without interruption. in both groups, the total duration of coronary occlusions each day was the same (90 minutes). before and after repetitive occlusions of either group, effect ...	1989	2912600
synthesis and copper-dependent antimycoplasmal activity of 1-amino-3-(2-pyridyl)isoquinoline derivatives. 2. amidines.	in our search for new compounds with antimycoplasmal activity, a series of aromatic amidines derived from 1-amino-3-(2-pyridyl)isoquinoline (1) was synthesized. in the presence of 40 microm copper the most active compounds show growth inhibition of mycoplasma gallisepticum in the nanomolar range. these compounds are 3 times as active as tylosin, an antimycoplasmal therapeutic agent that is used in veterinary practice. in the presence of copper, amidines derived from 1 are 2-3 times more active t ...	1989	2913309
improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin.	as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ...	1989	2914405
the artificial sphincter (as-800). experience in children and young adults.	from reviewing our experience in the application of the as-800 sphincter for the management of incontinence in children, we conclude that implantation is indicated after conservative and less invasive therapy has been attempted. the bladder neck is the site of choice for cuff placement in children. primary deactivation and moderate or low reservoir pressures (61 to 70 and 71 to 80 cm h2o) are indicated, especially in patients with previous bladder neck surgery or an inadvertent bladder neck inju ...	2010	2916269
potassium secretion by the cortical collecting tubule: effect of c1 gradients and ouabain.	in various epithelia k and cl transport are molecularly coupled and kcl cotransport is dependent on the na-k pump. the present study examines 1) the effect of a bath-to-lumen cl gradient on k secretion during active na transport and 2) the effect of basolateral ouabain on k secretion and na absorption in the presence and the absence of a bath-to-lumen cl gradient. under symmetrical conditions there was significant k secretion (jk = -24.0 +/- 3.9 pmol.mm-1.min-1) and cl secretion (jcl = -15.7 +/- ...	1989	2916662
pyocele of scrotum: consequence of spontaneous rupture of testicular abscess.	we report 2 cases of spontaneous rupture of the testis secondary to suppurative epididymo-orchitis. both cases presented with pyoceles and were treated conservatively by scrotal incision and drainage of the pyoceles. this prolonged hospital stay and increased morbidity, since both patients ultimately required orchiectomy. we believe that a pyocele is an indication for prompt surgical exploration, and if the testis is ruptured, an orchiectomy should be done.	2010	2929065
temporal lobe pathology in schizophrenia: a quantitative magnetic resonance imaging study.	although numerous studies have confirmed the presence of larger cerebral ventricles in schizophrenia, the locus of tissue loss remains elusive. by analyzing magnetic resonance scans with computerized image analysis, the authors determined gray and white matter volumes in the temporal lobes and prefrontal regions of 17 patients with schizophrenia and 17 age- and sex-matched normal subjects. the volume of temporal lobe gray matter was 20% smaller in the patients than in the control subjects. the l ...	2010	2929746
fixed drug erythema due to two unrelated drugs.		2010	2935136
thromboxane synthetase inhibitors: design and synthesis of a novel series of omega-pyridylalkenoic acids.		2010	2936112
segmental flexibility of receptor-bound immunoglobulin e.	the segmental flexibility of mouse immunoglobulin e (ige) bound to its high-affinity receptor on membrane vesicles from rat basophilic leukemia cells was compared to that of ige in solution by measuring the steady-state anisotropy as a function of temperature and viscosity. a monoclonal ige was used to bind the fluorescent probe n-[5-(dimethylamino)naphthalene-1-sulfonyl]-l-lysine (dns-lys) rigidly and specifically in the antigen combining site at the tip of the fab region. the average rotationa ...	1985	2936392
intrathecally administered apomorphine or ly171555 reduces nociceptive responses recorded from ventrobasal thalamic neurones in urethane anaesthetised rats.	the effects of intrathecal injections of the d2 agonists apomorphine and ly171555 were studied on responses of ventrobasal neurones responding to noxious stimulation in the urethane anaesthetised rat. at a dose of 75 micrograms/kg the d2 agonists had little effect on a total of 18 thalamic neurones. a higher dose (100 micrograms/kg) produced a reversible reduction of thalamic nociceptive responses on 15 occasions. a transient fall in blood pressure usually accompanied the intrathecal injection o ...	1987	2953995
affinity purification of hexosaminidases.	hexosaminidases a and b were purified by affinity chromatography from normal gastric mucosa, after preliminary separation of isozymes by anion exchange chromatography. heparin and mannosamine were coupled to sepharose 4b and used as affinity matrices and the purified enzymes were found to be homogeneous when analysed by polyacrylamide slab gel electrophoresis. this combination of 2 novel affinity chromatographic procedures is superior to existing methods in that a final yield of over 70% could b ...	2010	2965176
a prospective study of platelets and plasma proteolytic systems during the early stages of rocky mountain spotted fever.	we prospectively examined early changes in platelets and plasma proteolytic systems in 12 vaccinated and 6 unvaccinated volunteers in whom rocky mountain spotted fever developed after challenge with rickettsia rickettsii. the platelet counts declined while the plasma concentration of beta-thromboglobulin and the ratio of beta-thromboglobulin to platelet factor 4 increased, indicating in vivo activation of platelets. plasma levels of antithrombin iii decreased and levels of fibrinopeptide a incre ...	1988	2965302
physical disability: the role of the physical scientist in the health service. a report of the institute of physical sciences in medicine.	the report of the royal college of physicians on physical disability in 1986 and beyond gives details of a medical disability service to redress the present imbalance in the provisions for the physically disabled. an integral part of this service should be the provision of adequate scientific and technical resources, which could be established by making four or five new medical physics and clinical engineering appointments annually in each region for the next ten years.	2010	2966028
cell-mediated immunity and symptomatic diabetic autonomic neuropathy.	t lymphocytes have been implicated in the nerve damage observed in allergic experimental neuritis and in idiopathic polyneuritis. symptomatic autonomic neuropathy in long-standing type 1 diabetes is a rare and unexplained complication, and some preliminary evidence has suggested a pathogenetic role for the immune system. we have measured levels of activated t lymphocytes in 18 type 1 diabetic patients with symptomatic autonomic neuropathy and in 16 matched patients with uncomplicated type 1 diab ...	2009	2976645
nimh to reorganize extramural research.		2010	2990049
[current trends in the development of the science of coagulation abroad].		2010	2996966
restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000.	a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ...	1985	3002238
restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000.	a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ...	1985	3002238
herpes simplex virus: a role in the aetiology of alzheimer's disease?		1986	3005513
the interaction of recombinant huifn-gamma 1 and recombinant huifn-alpha 2 on the 2-5a system in two distinct cell types.		1985	3008171
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
human papillomavirus types 16 and 18 sequences in early cervical neoplasia.	a total of 100 colposcopic biopsies from patients with abnormal papanicolau's tests were surveyed for the presence of human papillomavirus (hpv) types 16 and 18 sequences by spot-blot hybridization. hpv 16 and 18 dna sequences were detected in 58% of the biopsies. none of the cervical intraepithelial neoplasia grade i (cin i) contained hpv 16 while 50% of the cin iii lesions (carcinoma in situ, cis) contained hpv 16. hpv 18-related sequences were equally represented in cin i, ii, and iii. southe ...	1986	3022464
prevalence and distribution of spotted fever and typhus infections in sierra leone and ivory coast.	a serosurvey for evidence of rickettsial infections was conducted in the rural populations of several tropical rain forest areas in sierra leone and ivory coast. seropositivity rates were surprisingly high in both countries, with more than 7% of the individuals in some districts having antibodies to spotted fever-group rickettsiae. no significant difference was found in the overall prevalence of diagnostic antibody titers to spotted fever-group rickettsiae in sierra leone (5.3%) and ivory coast ...	1986	3025125
[oncologic viewpoints of surgical and internal medicine cooperation].	chemotherapy is the treatment of first choice in small cell lung cancer. retrospective analysis and preliminary results of prospective studies demonstrate that an additional local treatment will increase the incidence of two years recurrence free survival. this role would be taken over by surgical resection for patients with the very limited stages i and ii. surgery has the advantage as compared to radiotherapy of exact exploration of the thorax and analysis of lung tissue after chemotherapy. fu ...	2011	3027472
viral specificity of multiple sclerosis tear immunoglobulins.	the mucosal immune system plays an important role in determining how an individual handles infection by most of the common viruses. tears form part of this system and participate in mucosal immunity. we examined tears from multiple sclerosis (ms) and control subjects for antibodies to seven common neurotropic viruses. antibodies to multiple viruses were detected in ms tears, especially when tears were obtained from chronic progressive active patients. in particular, ms patients showed a signific ...	1987	3029176
[effect of cholera enterotoxin on carbohydrate metabolism of the liver and small intestine mucosa in the rabbit].	changes in carbohydrate metabolism were studied in the isolated intestinal loops of rabbits during secretory diarrhea, induced by cholera enterotoxin. glucose synthesis level in the small intestinal mucosa and liver was measured by isotope technique, using l-alanine as a precursor. intestinal gluconeogenesis, calculated per mg of protein, appeared to be twice higher than in the liver of fasting rabbits. cholera enterotoxin administration enhanced gluconeogenesis in the liver by 60%, as compared ...	1987	3032299
molecular cloning of cdnas of human liver and placenta nadh-cytochrome b5 reductase.	a cdna coding for human liver nadh-cytochrome b5 reductase (cytochrome b5 reductase, ec 1.6.2.2) was cloned from a human liver cdna library constructed in phage lambda gt11. the library was screened by using an affinity-purified rabbit antibody against nadh-cytochrome b5 reductase of human erythrocytes. a cdna about 1.3 kilobase pairs long was isolated. by using the cdna as a probe, another cdna (pb5r141) of 1817 base pairs was isolated that hybridized with a synthetic oligonucleotide encoding p ...	1987	3035541
[x-ray computed tomography in venous pathology].	after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed.	2011	3043474
the release of pituitary lh and sprouting of lh-releasing hormone (lhrh)-containing neurons after anterior hypothalamic deafferentation.	the chronology of changes in plasma luteinizing hormone (lh) and the distribution of immunoreactive neuronal processes containing lh releasing hormone (lhrh-ir) were studied in the female rat after surgical interruption of anterior neural connections of the mediobasal hypothalamus (mbh). spontaneous lh surges on the afternoon of proestrus and lh release after estradiol benzoate (eb) followed 48 h later by progesterone (p) administration were studied in ovariectomized (ovx) rats. the maximum incr ...	1988	3044516
[the respiratory tract and air pollution].		2008	3053144
characterization of mannoproteins from a virulent candida albicans strain and its derived, avirulent strain.	antigenic differences between a wild-type virulent strain of candida albicans 4918 (wt) and its spontaneous avirulent mutant (m-10) were found with crossed-immunoelectrophoresis and western blotting. mannoprotein fractions from yeast and mycelial forms were obtained by affinity chromatography on concanavalin a and analyzed with use of patient sera or sera from rabbits infected with live wt cells and boosted with whole extracts or rabbits immunized with purified wt cell wall preparations. a spike ...	1988	3055210
cyclophosphamide-induced liver necrosis: a possible interaction with azathioprine.	cyclophosphamide is a valuable drug for the treatment of vasculitis and hepatic toxicity resulting from its use has been reported rarely. we describe four patients in whom cyclophosphamide was associated with liver injury when its administration had been preceded by azathioprine. microscopy of hepatic biopsy material in three of these cases showed liver cell necrosis. in two of the patients, cyclophosphamide had been given previously without earlier azathioprine and hepatic damage had not been n ...	2011	3060893
psychological and somatic factors in women with pain due to pelvic congestion.	chronic pelvic pain in association with dilated pelvic veins and polycystic ovaries is a common complaint in women in the reproductive years, regardless of whether or not they have been pregnant. these women have a higher incidence of deaths and illnesses amongst family members and close relatives which makes them pay more attention to physical complaints such as pain which may make them more susceptible to environmental "stress" of daily life. a hypothesis is put forward which would link the ef ...	2011	3067564
asthma and nifedipine. comparison of nifedipine, ketotifen and placebo in the prophylaxis of childhood extrinsic asthma.	these results were obtained on a double blinded study which compared the nifedipine effects, a dihydropyrine calcium blocker of extensive cardiovascular function, the ketotifen and a placebo orally administered, during 4 months, every 12 hours, on children with allergic asthma, older than 5 years old, who had a minimum of 2 years of evolution in their sickness. these people consulted the allergic service of the university hospital of san vicente de paul in medellín, colombia, between july of 198 ...	2011	3067566
elbow pain.	nonarticular causes of elbow pain include muscle strains, ligamentous injuries, epicondylitis, olecranon bursitis, and compressive neuropathies. overuse and trauma commonly cause these conditions. the history and physical examination differentiate them from an intra-articular process such as synovitis. laboratory analysis of fluid aspirated from a swollen olecranon bursa is necessary to differentiate infection or gout. x-rays are useful in avulsion fracture, osteochondritis dissecans, and epiphy ...	2010	3068691
eftu provides an internal kinetic standard for translational accuracy.		2010	3072707
antigenic heterogeneity in high- and low-virulence strains of rickettsia rickettsii revealed by monoclonal antibodies.	previously it has been reported that strains of rickettsia rickettsii that differ greatly in their ability to cause disease in guinea pigs are similar by serological and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses. in this study, we used monoclonal antibodies to the virulent r and the relatively avirulent hlp strains to investigate strain differences which might account for the disparate behavior of the strains in guinea pigs. coomassie blue-stained sodium dodecyl sulfate- ...	1986	3080372
ecological study of rocky mountain spotted fever in costa rica.	since rocky mountain spotted fever (rmsf) was recognized in costa rica, ecological studies have been undertaken to discern its distribution and the natural cycle of rickettsia rickettsii. the population of ticks parasitizing animals and vegetation was scarce. rickettsiae were not isolated from pools of ticks nor from spleens of the animals studied. however, the active circulation of rickettsiae was evidenced through the demonstration of spotted fever group antibodies in humans, dogs, and wild ra ...	1986	3080917
actions of antibiotics on rickettsia rickettsii.		1986	3081659
adherence of platelets to human endothelial cells infected by rickettsia rickettsii.	rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever, causes widespread vasculitis due to extensive injury of endothelial cells lining small blood vessels. injury to these cells can lead to denudation of the endothelial lining and exposure of the thrombogenic subendothelium. modification of the cell surface, either through minor trauma or as a result of altered cell surface biochemistry, could induce changes reflected in increased platelet reactivity. because membrane modif ...	1986	3081662
humoral immune response to rocky mountain spotted fever in experimentally infected guinea pigs: immunoprecipitation of lactoperoxidase 125i-labeled proteins and detection of soluble antigens of rickettsia rickettsii.	rickettsia rickettsii, the etiologic agent of rocky mountain spotted fever, purified from infected l-929 cells by density gradient banding were extrinsically radioiodinated with lactoperoxidase. immunodominant 125i-labeled antigens were identified by radioimmunoprecipitation of detergent-solubilized antigens with protein a-sepharose and anti-r. rickettsii sera collected 0, 3, 7, 11, 32, and 163 days after infection of guinea pigs. the average fever greater than or equal to 40 degrees c was detec ...	1986	3082753
production and characterization of cloned t-cell hybridomas that are responsive to rickettsia conorii antigens.	t-cell hybridomas produced by the fusion of rickettsia conorii immune t cells to the akr thymoma bw 5147 produced interleukin-2 when stimulated with the antigens of three different r. conorii strains. one cloned hybridoma responded only to r. conorii antigens, whereas a second and third cloned hybridoma also responded to the antigens of rickettsia rickettsii sheila smith and rickettsia sibirica 246, respectively. antigen responses required antigen-presenting cells, and this interaction was major ...	1986	3082761
the microbiologic etiology of periodontal disease.		2011	3087693
challenging conventional aminoglycoside dosing regimens. the value of experimental models.	although newer antimicrobials look promising for the treatment of serious gram-negative infections, aminoglycosides still remain part of the mainstay of their therapy. traditional intermittent therapy is based upon the premise that high serum aminoglycoside concentrations are toxic. however, the rate of bacterial killing for aminoglycosides is also a concentration-dependent phenomenon. two animal models of pseudomonas pneumonia were utilized to examine the efficacy of non-traditional aminoglycos ...	1986	3089001
the sensitivity of various serologic tests in the diagnosis of rocky mountain spotted fever.	sensitivity indices for various serologic tests in the diagnosis of rocky mountain spotted fever (rmsf) were determined from rmsf surveillance data collected at the centers for disease control (cdc) during 1981-1984. during these 4 years, a total of 4,141 cases of rmsf in the u.s. were reported to cdc. case report forms, which include information on dates and results of serologic testing, were received for 3,567 of these cases; 1,774 were laboratory-confirmed according to criteria published prev ...	1986	3089042
[serologic studies of spotted fever group rickettsiosis occurring in muroto-city, kochi prefecture].		1986	3090165
[better integration of the student into the activities of the ward. goal-oriented integration during the first week is of decisive importance].		2011	3090364
[fluoride prevention--concerning the health risk].		2011	3090743
effects of the binding of myosin light chain kinase on the reactivities of calmodulin lysines.	the effects of the binding of smooth muscle myosin light chain (mlc) kinase on the microenvironments of different regions of calmodulin (cam) were investigated by comparing the acylation rate constants of the seven lysine amino groups of free cam with those of cam complexed with mlc kinase. equimolar amounts of cam and cam-mlc kinase complex were trace labeled with [3h]acetic anhydride in the presence of phenylalanine as a standard nucleophile. after completion of the reaction, equal amounts of ...	1986	3091599
radioimmunoprecipitation of [35s]methionine-radiolabeled proteins of rickettsia conorii and rickettsia rickettsii.		1986	3091709
the contribution of large granular lymphocytes to b cell activation and differentiation after t-cell-depleted allogeneic bone marrow transplantation.	immunoglobulin and specific antibody levels are well maintained in the recipients of t-cell-depleted allogeneic bone marrow transplants (bmt), even though up to 99% of mature t cells are removed from the donor graft. for 3-8 weeks after the procedure, natural killer (nk) cells with an activated pattern of target cell killing have been shown to circulate in the recipient. this study investigates whether these recipient nk cells spontaneously secrete lymphokines that modulate b cell function in a ...	2011	3092411