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analysis by the reductive-cleavage method of linkage positions in a polysaccharide containing 4-linked d-glucopyranosyluronic residues.the fate of 4-linked d-glucopyranosyluronic residues under reductive-cleavage conditions was investigated by using the klebsiella aerogenes type 54 strain a3 capsular polysaccharide. treatment of the fully methylated polysaccharide with triethylsilane and trimethylsilyl trifluoromethanesulfonate in dichloromethane, followed by in situ acetylation, yielded 1,5-anhydro-2,3,4,6-tetra-o-methyl-d-glucitol, 3,4-di-o-acetyl-1,5-anhydro-2,6-di-o-methyl-d-glucitol, and 3-o-acetyl-1,5-anhydro-2,4-di-o-met ...19883288343
common evolutionary origin of the ilvgmeda attenuation locus and trna(1leu) in escherichia coli.published sequences of transcripts from ilvgmeda leader regions of several enteric bacteria were compared with published sequences of the trnas from escherichia coli. the analyses revealed homology between the ilvgmeda leader peptide-coding region and trna(1leu) in e. coli, salmonella typhimurium, and klebsiella aerogenes, whereas homology was not present in serratia marcescens and edwardsiella tarda.19873294812
evolution of the enterobacterial sula gene: a component of the sos system encoding an inhibitor of cell division.the lexa-regulated sula (sfia) gene of escherichia coli encodes an unstable protein which inhibits cell division. by determining the nucleotide sequences of the corresponding genes from the related bacteria salmonella typhimurium, enterobacter aerogenes and serratia marcescens it was found that the regulatory region and the lexa binding site (sos box) have been better conserved during evolution than the coding sequence. the n terminus of the sula protein [amino acid (aa) residues 1-30] has diver ...19873297925
incidence of staphylococcus aureus, coliforms and antibiotic-resistant strains of escherichia coli in rural water supplies in port harcourt.the bacteriological quality of some rural water supplies in port harcourt was monitored over a 3 month period. the supplies were unsatisfactory as judged by standard plate counts (10(3)/ml) and the presence of presumptive and faecal coliforms and staphylococcus aureus. the recovery of potentially pathogenic bacteria (e.g. pseudomonas aeruginosa) further substantiated the existence of health hazards. the most frequently isolated coliforms were escherichia coli, enterobacter aerogenes and klebsiel ...19873298188
biosynthesis of o-succinylbenzoic acid. i: cell free synthesis of o-succinylbenzoic acid from isochorismic acid in enzyme preparations from vitamin k producing bacteria.escherichia coli k12 and a mutant of e. coli (viz., an 154) as well as aerobacter aerogenes 62-1 (i.e., klebsiella pneumoniae) were used as sources of the enzyme catalyzing the formation of o-succinylbenzoic acid (osb) from isochorismic acid and alpha-ketoglutaric acid in the presence of thiamine pyrophosphate. the product of the reaction (osb) was identified by hplc before and after derivatization to the methylester, dilactone, and coenzyme a ester. osb synthase and alpha-ketoglutarate dehydrog ...19873300552
electron microscopical investigation of citrate lyase single molecules.electron micrographs of citrate lyase from rhodopseudomonas gelatinosa and klebsiella aerogenes reveal two characteristic molecular forms. the "basket" form and the "star" form were subjected to two-dimensional image reconstruction using a technique involving averaging of superposed single molecular images after rotational correlation. a three-dimensional image reconstruction shows that the images of these forms can be interconverted by rotation and that they therefore represent different views ...19873304340
the k1 beta-lactamase of klebsiella pneumoniae.beta-lactamase k1 was purified from klebsiella pneumoniae sc10436. it is very similar to the enzyme produced by klebsiella aerogenes 1082e and described by emanuel, gagnon & waley [biochem. j. (1986) 234, 343-347]. an active-site peptide was isolated after labelling of the enzyme with tritiated beta-iodopenicillanate. a cysteine residue was found just before the active-site serine residue. this result could explain the properties of the enzyme after modification by thiol-blocking reagents. the s ...19873307765
isolation from klebsiella and characterization of two rcs genes that activate colanic acid capsular biosynthesis in escherichia coli.two genes, designated rcsa (regulation of capsule synthesis) and rcsb, that had been cloned from the chromosome of klebsiella aerogenes (k. pneumoniae) capsular serotype k21 were capable of activating expression of colanic acid capsular polysaccharide in escherichia coli k12. the klebsiella rcsa gene encoded a polypeptide of 23 kda that was required for the induction of a mucoid phenotype at less than or equal to 30 degrees c but not at greater than or equal to 37 c. the klebsiella rcsb locus en ...19873309150
evaluation of cellulose acetate/nitrate filters for the study of stallion sperm motility.stallion semen was diluted in a hepes-supplemented buffer (cm) (10(6) spermatozoa/ml) and placed in the upper well of a sykes-moore chemotaxis chamber. chambers were incubated in a humidified atmosphere (5% co2 in air) at 37 degrees c for 1 and 2 h and spermatozoa were allowed to swim through filters with a mean pore size of 3,5 or 8 micron. spermatozoa entered filters of all three pore sizes. distance travelled was greater for each increase in pore size (p less than 0.01) but did not differ (p ...19873316643
the role of capsular polysaccharide k21b of klebsiella and of the structurally related colanic-acid polysaccharide of escherichia coli in resistance to phagocytosis and serum killing.the behaviour of strains of klebsiella aerogenes of capsular serotype k21 and strains of escherichia coli producing a structurally related polysaccharide (colanic acid) was analysed by phagocytic and serum-killing assays. the cell-surface characteristics of these strains and of non-capsulate strains derived from them were also investigated by partitioning experiments in aqueous two-polymer phase systems. the possession of k21-type capsule by k. aerogenes or colanic-acid polysaccharide by e. coli ...19873320374
[clinical laboratory approach for estimating the effective administrative dose of latamoxef. significance of a 4-category system interpretation of the latamoxef disc susceptibility test].in vitro activities of latamoxef (lmox) against 249 clinical isolates were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. lmox was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis (except 2 out of 30 strains) and proteus vulgaris with mic values below 3.13 micrograms/ml. it was also active against enterobacter aerogenes with mic80 of 1.56 micrograms/ml. lmox was less active against serratia marcescens, inhibiting about 43% of strai ...19873327966
ultrasound-guided brain abscess aspiration in neonates.four cases of brain abscess in neonates are described, diagnosed by ultrasonography and ct. all abscesses were confirmed surgically. one patient was operated on 5 weeks after diagnosis because of initial parental refusal. the etiology in all cases was meningitis superimposed on an hypoxic-ischemic insult. two cases had a single abscess while the other two had multiple lesions. all cases were operated on with intraoperative ultrasound examination through the fontanelle. the case with delayed aspi ...19873329960
interactions between antibacterial agents of the quinolone group and nitrofurantoin.plate diffusion tests showed that nitrofurantoin antagonized the activity of nalidixic acid, norfloxacin, ciprofloxacin and enoxacin against many gram-negative bacilli, including all proteus mirabilis and klebsiella aerogenes strains tested. no interaction was exhibited when nitrofurantoin and the newer quinolones were tested against pseudomonas aeruginosa, staphylococci or streptococci. antagonism was not reliably demonstrated in chessboard titrations, especially with the newer quinolones. cont ...19883356622
cloning and nucleotide sequence of the isoamylase gene from pseudomonas amyloderamosa sb-15.the gene (iam) coding for isoamylase (glycogen 6-glucanohydrolase) of pseudomonas amyloderamosa sb-15 was cloned. its nucleotide sequence contained an open reading frame of 2313 nucleotides (771 amino acids) encoding a precursor of secreted isoamylase. the precursor contained a signal peptide of 26 amino acid residues at its amino terminus and three regions homologous with those conserved in alpha-amylases (1,4-alpha-d-glucan 4-glucanohydrolase) of species ranging from prokaryotes to eukaryotes. ...19883379068
risk factors for development of sepsis in a hospital outbreak of enterobacter aerogenes. 19883408015
formation of 6-o-alpha-maltosylcyclomalto-oligosaccharides by transfer action of three debranching enzymes.o-maltosylcyclomaltohexaoses (g2-cg6) were formed in yields of 24.3 and 23.2 mmol from 40 mmol of alpha-maltosyl fluoride (alpha-g2f) and 90 mmol of cyclomaltohexaose (cg6) by the transfer action of pullulanase from aerobacter aerogenes (a-pullulanase) and isoamylase from pseudomonas amyloderamosa, respectively. these yields were three times that given by pullulanase from bacillus acidopullulyticus (b-pullulanase). the yields of o-maltosylcyclomalto-oligosaccharides were changed according to the ...19873427582
isolation and characterization of the m.eaei modification methylase.the modification enzyme (m.eaei) corresponding to the restriction endonuclease eaei was partially purified from enterobacter aerogenes pw201. the m.eaei enzyme methylates the innermost cytosine residue in each strand of the family of related sequences that constitute the eaei recognition site to give: 5'-y-g-g-5mc-c-r-3' where 5mc is 5-methylcytosine. m.eaei protects these sites against cleavage by haeiii, and protects overlapping 5'-c-c-g-g-3' sites against cleavage by both hpaii and mspi.19863467722
antimicrobial activity of imipenem against 1386 clinical isolates.1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...19863468319
comparison of difloxacin, enoxacin, and cefoperazone for treatment of experimental enterobacter aerogenes endocarditis.this study compared difloxacin administered orally, enoxacin administered orally, and cefoperazone administered intramuscularly for the treatment of experimental enterobacter aerogenes endocarditis. difloxacin significantly reduced bacterial counts of vegetations, as compared with enoxacin and cefoperazone. enoxacin and cefoperazone did not differ significantly. this study demonstrated that difloxacin was significantly more effective than enoxacin and cefoperazone for the treatment of e. aerogen ...19873472489
[clinical laboratory approach for estimating effective administrative dose of ceftizoxime. observation from mic and ceftizoxime disc susceptibility test].in vitro activities of ceftizoxime (czx) against 328 clinical isolates were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. czx was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis, and proteus vulgaris with mic values below 0.20 microgram/ml. it was also active against serratia marcescens and enterobacter aerogenes with mic85 of 3.13 micrograms/ml. czx was less active against staphylococcus aureus and staphylococcus epidermidis, sh ...19873474430
novel plasmid-mediated beta-lactamase in members of the family enterobacteriaceae from ohio.epidemiologic studies of plasmid-mediated resistance at the cleveland veterans administration medical center revealed that related plasmids had disseminated among members of the family enterobacteriaceae. we studied the beta-lactamases encoded by these plasmids in escherichia coli c600 transformants or transconjugants. substrate and inhibition profiles of the enzymes determined by two of these plasmids suggested an activity resembling tem-1; however, isoelectric focusing revealed a pi of 7.0. th ...19863490215
in vitro activity and beta-lactamase stability of a new difluoro oxacephem, 6315-s.6315-s, a novel difluoromethyl thioacetamido oxacephem, had in vitro activity comparable to that of cefotaxime and moxalactam against escherichia coli, klebsiella pneumoniae, proteus mirabilis, klebsiella oxytoca, citrobacter diversus, salmonella spp., and shigella spp., inhibiting 90% at less than or equal to 0.25 microgram/ml. it inhibited piperacillin- and cefoperazone-resistant isolates in these species. 6315-s did not inhibit cefotaxime- or moxalactam-resistant citrobacter freundii, enterob ...19863492172
passive immunization of mice against klebsiella aerogenes.klebsiella vaccines were isolated by mild diafiltration techniques from culture filtrates of nine capsular types of k. aerogenes (k1, k2, k3, k15, k20, k35, k36, k44 & k63). the bacteria were grown in a chemically defined medium in standardized conditions in a fermenter. the vaccines had a molecular weight of more than 20 000, a carbohydrate content of 40-89%, a protein content of between less than 1 and 16% and small amounts (0.6-1.2%) of lipopolysaccharide. antisera raised in rabbits to the ni ...19863511939
studies on microbial quality of filtered water in households of a university community in nigeria.water samples from home filters in nine residential areas of a nigerian university community were studied. the membrane filter technique was used to determine the total coliform and faecal coliform counts/100 ml of water. most of the 100 samples studied were grossly contaminated with total coliform counts/100 ml ranging from 0-442, faecal coliform counts/100 ml, 0-216 and the total aerobic plate count per millilitre ranged from 3.0 x 10(3) to 1.9 x 10(9) c.f.u. the source (dams) of water, fitnes ...19863517156
preparation, characterization, and reconstitution of oxaloacetate decarboxylase from klebsiella aerogenes, a sodium pump. 19863520232
structural and kinetic studies on beta-lactamase k1 from klebsiella aerogenes.beta-lactamase k1 from klebsiella aerogenes 1082e hydrolyses both penicillins and cephalosporins comparably and is inhibited by mercurials but not by cloxacillin. these properties distinguish it from those other beta-lactamases that have been allotted to classes on the basis of their amino sequences. beta-lactamase k1 has been isolated by affinity chromatography; its composition shows resemblances to class a beta-lactamases. moreover, the n-terminal sequence is similar to those of class a beta-l ...19863521585
bacterial antagonists of shigellae.bacterial antagonism between a microorganisms and shigella sonnei strains was studied in model experiments simulating conditions of the natural aquatic environment. in these studies surface waste samples from the river vltava served as the experimental environment. to ensure bacteriologically defined conditions all water samples were heat-sterilized prior to antagonism testing. consistently with the literature data and author's own observations the following bacterial species and genera were cho ...19863522730
affinity of cephalosporins for beta-lactamases as a factor in antibacterial efficacy.strains of escherichia coli, enterobacter aerogenes, and enterobacter cloacae that were resistant to ceftazidime (mic greater than 16 micrograms/ml) but susceptible to bmy 28142 (mic less than 4 micrograms/ml) were found to contain higher levels of beta-lactamase activity (50- to 3,340-fold) than control strains of the corresponding species. ceftazidime was at least as resistant as bmy 28142 to hydrolysis by these enzymes. however, the apparent ki of bmy 28142 for each enzyme was larger (8- to g ...19863524432
r plasmids, kr61 and kr61-a, from aerobacter aerogenes. 19863525399
[effect of cationic polymers and surface-active substances on bacterial resistance to kanamycin].the effect of cationic polymers and surface active substances (sas) on sensitivity of klebsiella aerogenes 600, escherichia coli 154 and proteus vulgaris 7470 to kanamycin was studied. a decrease in the resistance of the above organisms to kanamycin on its use in combination with cationic polymers and sas was observed. it was shown that such substances inhibited the activity of the enzymes inactivating kanamycin. their effect was suggested to be due to changes in the lipid surrounding of the enz ...19863527060
magnesium-dependent plaque formation by bacteriophage p1cinc(-) on escherichia coli c and shigella sonnei.phage p1c(-), in a state of the phage not infective to escherichia coli k12, was able to form plaques on a wild-type strain of e. coli c and on shigella sonnei in the presence of mg2+. citrobacter freundii, enterobacter aerogenes, and a salmonella typhimurium gale mutant were not lysed by, but were lysogenized with p1cinc(-), whereas klebsiella pneumoniae, proteus rettgeri, and s. typhimurium lt2 were not susceptible to either p1cinc(-) or p1cinc(+). the lipopolysaccharide structure of e. coli c ...19863535235
bacteria and helminth isolates from bile and faeces of zebu cattle slaughtered for human consumption in the niger delta areas of nigeria.random bacteriological examination of 300 bile samples aseptically drawn from the gallbladders of adult cattle at slaughter revealed the presence of escherichia coli in 30%, enterobacter aerogenes in 14% and klebsiella aerogenes in 56%. bacteriological examination of another 116 faecal samples revealed esch. coli in 60% and ent. aerogenes in 40%, while parasitological examination revealed 50-100 strongyle ova per gram from pre-rain samples of faeces, 100-850 strongyle eggs per gram for faeces co ...19863539044
part of respiratory nitrate reductase of klebsiella aerogenes is intimately associated with the peptidoglycan.lysozyme digestion and sonication of sodium dodecyl sulfate (sds)-purified klebsiella aerogenes murein sacculi resulted in the quantitative release of both subunits of nitrate reductase, as well as a number of other cytoplasmic membrane polypeptides (5.2%, by weight, of the total membrane proteins). similar results were obtained after lysozyme digestion of sds-prepared peptidoglycan fragments, which excluded the phenomenon of simple trapping of the polypeptides by the surrounding peptidoglycan m ...19873542973
evaluation of a commercial beta-glucuronidase test for the rapid and economical identification of escherichia coli.a commercial beta-glucuronidase (beta-gur) test for the rapid and economical identification of escherichia coli was evaluated. a total of 762 clinical strains and 228 environmental isolates were studied. more than 95% of the e. coli strains were found to be beta-gur positive. thirty-one clinical isolates of shigella sonnei, 10 of enterobacter cloacae, eight of enterobacter aerogenes, nine of citrobacter freundii and one of salmonella enteritidis also gave positive results. the enzyme beta-gur wa ...19863549665
five-year survey of changing patterns of susceptibility of bacterial uropathogens to trimethoprim-sulfamethoxazole and other antimicrobial agents.we analyzed the antibiotic susceptibility of 5,348 urinary isolates of escherichia coli, "klebsiella aerogenes," and proteus mirabilis grown in three laboratories from 1980 to 1985. a continuous rise in resistance to trimethoprim-sulfamethoxazole was observed; 63% of the strains from inpatients in 1984 and 51% of those from outpatients in 1985 were resistant to this drug. isolates from outpatients in 1985 were mostly susceptible to nitrofurantoin (mean susceptibility, 92%) and to oral cephalospo ...19873551824
antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (bw a509u).the thymidine analog 3'-azido-3'-deoxythymidine (bw a509u; azidothymidine [azt]) had potent bactericidal activity against many members of the family enterobacteriaceae, including strains of escherichia coli, salmonella typhimurium, klebsiella pneumoniae, shigella flexneri, and enterobacter aerogenes. azt also had bactericidal activity against vibrio cholerae and the fish pathogen vibrio anguillarum. azt had no activity against pseudomonas aeruginosa, gram-positive bacteria, anaerobic bacteria, m ...19873551832
purification and characterization of the nickel-containing multicomponent urease from klebsiella aerogenes.klebsiella aerogenes urease was purified 1,070-fold with a 25% yield by a simple procedure involving deae-sepharose, phenyl-sepharose, mono q, and superose 6 chromatographies. the enzyme preparation was comprised of three polypeptides with estimated mr = 72,000, 11,000, and 9,000 in a alpha 2 beta 4 gamma 4 quaternary structure. the three components remained associated during native gel electrophoresis, mono q chromatography, and superose 6 chromatography despite the presence of thiols, glycols, ...19873553184
treatment of chronic bacterial prostatitis with ciprofloxacin. results of a one-year follow-up study.in a one-year follow-up study, short-term therapy with ciprofloxacin proved to be beneficial in the treatment of chronic bacterial prostatitis, particularly in cases of escherichia coli prostatitis. ciprofloxacin therapy of e. coli prostatitis was a complete success in five of 12 patients, a probable success in two, and in one patient the outcome cannot be judged. ciprofloxacin treatment failed in three men, and therapy was discontinued in one patient because of side effects. enterobacter aeroge ...19873555048
oral ciprofloxacin in the treatment of elderly patients with complicated urinary tract infections due to trimethoprim/sulfamethoxazole-resistant bacteria.the effectiveness and safety of ciprofloxacin, a new quinolone antibiotic, were prospectively evaluated in the treatment of patients with complicated urinary tract infections caused by gram-negative organisms resistant to trimethoprim/sulfamethoxazole. twenty-five elderly (mean age, 70.4 years) patients (24 men and one woman) were enrolled. initial pathogens included pseudomonas aeruginosa (15 isolates), escherichia coli (five isolates), enterobacter aerogenes (one isolate), citrobacter freundii ...19873555051
detection of pullulanase in polyacrylamide gels using pullulan-reactive red agar plates.after electrophoresis, active pullulanase bands in acrylamide gels have been detected by overlaying and then incubating the gel on a replica gel containing 2.5% pullulan-reactive red conjugate and 2.1% agar. the enzyme activity is revealed as a clear band against a red background on the replica gel. the sensitivity of the replica plate is such that 0.0012 unit of klebsiella aerogenes pullulanase can be detected easily. this procedure is effective in enzyme screening to distinguish pullulanase fr ...19873555152
biosynthesis of thiamin. different biosynthetic routes of the thiazole moiety of thiamin in aerobic organisms and anaerobic organisms.the nitrogen atom of glycine was incorporated into the thiazole moiety of thiamin in the aerobic microorganisms bacillus subtilis, pseudomonas putida, saccharomyces cerevisiae, mucor racemosus, neurospora crassa, and emericella nidulans. it was not incorporated in the case of the facultative anaerobic microorganisms escherichia coli and enterobacter aerogenes, which, however, did incorporate the nitrogen atom of tyrosine. these results show that aerobic microorganisms and facultative anaerobic m ...19873566774
growth of paramecium tetraurelia in bacterized, monoxenic cultures.wild type and mutant paramecium tetraurelia were grown in monoxenic cultures by first growing enterobacter aerogenes on a defined medium and then adding the paramecium to the stationary phase bacterial culture. the bacterial growth was proportional to the concentration of the carbon source (citrate), and the paramecium growth was dependent upon both the bacterial density and the starting density of paramecium. the behavior, electrophysiological properties, ciliary lipid composition, and growth c ...19873585813
in vitro activity of ro 15-8074 and ro 19-5247 in comparison to cefaclor and cefalexin.805 clinical isolates were investigated for their in vitro sensitivity against ro 15-8074 and ro 19-5247 in comparison to cefaclor and cefalexin in a serial dilution test on solid medium. ro 19-5247 had the strongest activity of all drugs tested against streptococci (except streptococcus faecalis) and was as active as cefaclor and cefalexin against most strains of staphylococcus aureus. ro 19-5247 was the only oral cephalosporin active against bordetella pertussis. it was on average 160 times mo ...19873596808
patients' endogenous flora as the source of "nosocomial" enterobacter in cardiac surgery.we prospectively studied enterobacter colonization in cardiac surgery patients receiving cefazolin prophylaxis. fifty-eight (67%) of 87 patients became colonized, 28 by the time of admission to a cardiac surgery intensive care unit. enterobacter cloacae was four times more prevalent than enterobacter aerogenes. we found increased enterobacter colonization, after prophylaxis, in 45% of surgery patients. none of 25 control patients, who underwent coronary angioplasty and received no antibiotic pro ...19873598234
the in-vitro activity of cefadroxil, and the interpretation of disc-susceptibility testing.minimum inhibitory concentrations (mics) of cefadroxil were determined for 749 defined clinically-significant bacteria isolated in a london teaching hospital and for 63 strains from an international collection of gram-negative bacilli. assuming a breakpoint of 16 mg/l, for the hospital isolates 81.8% of gram-negative bacilli and 83.4% of gram-positive cocci were sensitive. no significant difference between in-patient, out-patient or community-acquired isolates was found. ninety-five and a half p ...19873610900
isolation of vibrio fluvialis, and unusual pathogen in acute suppurative cholangitis.we report a case of acute suppurative cholangitis, from which were isolated enterobacter aerogenes and vibrio fluvialis. there have been no previous reports of the isolation of v. fluvialis in this disease. it is usually found in seawater and various seafoods and may cause acute diarrhea, but this is the first report in which it has been associated with acute suppurative cholangitis.19873631041
in-vitro activity of ticarcillin with and without clavulanic acid against clinical isolates of gram-positive and gram-negative bacteria.the in-vitro activity of ticarcillin with and without clavulanic acid was investigated against 285 freshly isolated clinical strains of gram-positive and gram-negative bacteria by the agar-dilution technique on mueller-hinton-agar. clavulanic acid had an excellent or moderate potentiating effect on the in-vitro activity of ticarcillin against staphylococci, escherichia coli, klebsiella spp., enterobacter aerogenes, proteus mirabilis, citrobacter spp., acinetobacter, haemophilus influenzae and ba ...19863636325
protocatechuate is not metabolized via catechol in enterobacter aerogenes.protocatechuate is generally metabolized in bacteria by direct oxygenative cleavage to produce beta-carboxymuconate. an exception to this pattern has been suggested by reports that protocatechuate might be metabolized by nonoxidative decarboxylation to catechol in enterobacter aerogenes. in the present investigation, analysis of mutant strains indicated that this proposed pathway did not make a significant contribution to protocatechuate metabolism in e. aerogenes because mutations blocking cate ...19873680179
structure of the extracellular gelling polysaccharide produced by enterobacter (ncib 11870) species.the gelling polysaccharide produced by a species of enterobacter (ncib 11870) contains l-fucose, d-glucose, and d-glucuronic acid in the ratios 1:2:1. analysis of the methylated and methylated, carboxyl-reduced polysaccharide revealed terminal non-reducing glucose, (1----3)-linked fucose, (1----3,1----4)-linked glucose, and (1----4)-linked glucuronic acid in the ratios 1:1:1.2:0.8. from the results of smith degradation of the polysaccharide and spectroscopic studies of the acidic tetra- and octa ...19863708628
naegleria fowleri and n. lovaniensis: differences in sensitivity to trimethoprim and other antifolates.the growth of naegleria fowleri cultures in a bcs medium was not affected either by trimethoprim at 400 micrograms/ml or by aminopterine, 3,5-diaminopterine and methotrexate at 500 micrograms/ml. n. lovaniensis propagation in the same medium was inhibited with 10 micrograms/ml of trimethoprim, 50 micrograms/ml methotrexate and 100 micrograms/ml 3,5-diaminopteridine. aminopterine was ineffective at a concentration of 500 micrograms/ml. the inhibitory effect of trimethoprim on n. lovaniensis cultu ...19863776315
osteoarticular infections in children with sickle cell disease.thirteen children with sickle cell disease were identified as having 14 episodes of osteoarticular infection in a review of 27 years' experience. there were eight episodes of osteomyelitis or osteoarthritis and six of suppurative arthritis alone. the etiologic agents in osteomyelitis or osteoarthritis were salmonella sp in four cases, escherichia coli in one, enterobacter aerogenes in one, staphylococcus aureus in one, and haemophilus influenzae type b in one. five of the cases with infection li ...19863786034
purification and characterization of quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus l.m.d. 79.41.quinoprotein glucose dehydrogenase (ec 1.1.99.17) from acinetobacter calcoaceticus l.m.d. 79.41 was purified to homogeneity. it is a basic protein with an isoelectric point of 9.5 and an mr of 94,000. denaturation yields two molecules of pqq/molecule and a protein with an mr of 48000, indicating that the enzyme consists of two subunits, which are probably identical because even numbers of aromatic amino acids were found. the oxidized enzyme form has an absorption maximum at 350 nm, and the reduc ...19863800975
the effect of the combination of erythromycin with new beta-lactam antibiotics against gram-negative aerobic respiratory pathogens.the effect of erythromycin on the in vitro activity of cefotaxime, ceftizoxime, cefoperazone, moxalactam, ceftazidime, cefmenoxime, aztreonam, imipenem, piperacillin, and gentamicin against 89 bacteria isolated from sputum and tracheal aspirates of patients admitted to intensive care units was evaluated. there were 30 pseudomonas aeruginosa, 27 klebsiella pneumoniae, 9 enterobacter cloacae, 5 escherichia coli, 4 enterobacter aerogenes, 3 each of klebsiella oxytoca, pseudomonas maltophilia, serra ...19863802298
efficacy of imipenem/cilastatin in endocarditis.imipenem, a potent new beta-lactam antibiotic, which is bactericidal against most pathogenic bacteria, and cilastatin, a dehydropeptidase inhibitor combined with imipenem to prevent the metabolism of imipenem in the kidney, were evaluated in the treatment of bacterial endocarditis. seventeen patients, including 14 who used intravenous drugs, were treated with imipenem/cilastatin in a dose of 500 mg each infused over 30 minutes every six hours. the mean duration of treatment was 29 days with a ra ...19853859210
enoxacin compared with cefoperazone for the treatment of experimental enterobacter aerogenes endocarditis.this study compared enoxacin administered orally with cefoperazone administered intramuscularly for the treatment of enterobacter aerogenes endocarditis in rabbits. the mics and mbcs of both enoxacin and cefoperazone for an inoculum of 10(5) cfu/ml of the e. aerogenes strain used were 0.8 micrograms/ml, respectively. with an inoculum of 10(8) organisms per ml, enoxacin at 2 and 5 micrograms/ml and cefoperazone at 60 and 155 micrograms/ml were effective in reducing titers of e. aerogenes in broth ...19853860186
influence of imipenem on the serum resistance of enterobacteriaceae.following growth in a subinhibitory concentration of imipenem and additional incubation in a 20% dilution of normal human serum (nhs) for 90 minutes, five of 12 serum-resistant strains of enterobacteriaceae showed a decrease in colony-forming units of two or more logs of growth compared with the control. two strains (of escherichia coli and enterobacter aerogenes) showed this phenomenon even with incubation in 5% nhs. treatment with imipenem did not change the serum resistance of the other seven ...19853863217
the activity of cefbuperazone, a 7 alpha-methoxy 7 beta acyl ureido cephalosporin.the activity of cefbuperazone, a 7 alpha-methoxy ureido cephalosporin, was determined against 726 clinical isolates. ninety percent of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, proteus mirabilis, enterobacter aerogenes, proteus vulgaris, morganella morganii, salmonella, and shigella species were inhibited by less than or equal to 6.3 micrograms/ml. cefbuperazone was more active than cefamandole, cefoxitin and piperacillin against these species. concentrat ...19853879464
in vivo synthesis of histidine by a cloned histidine ammonia-lyase in escherichia coli.histidine ammonia-lyase catalyzes the first step in histidine catabolism, the deamination of histidine to urocanate and ammonia. in vitro experiments have shown that histidine ammonia-lyase also can catalyze the reverse (amination) reaction, histidine synthesis, relatively efficiently under extreme reaction conditions (4 m nh4oh, ph 10). an escherichia coli hisb deletion strain was transformed with a pbr322 derivative plasmid (pcb101) containing the entire klebsiella aerogenes histidine utilizat ...19853884601
cloning of the pullulanase gene and overproduction of pullulanase in escherichia coli and klebsiella aerogenes.the pullulanase gene (pul) of klebsiella aerogenes was cloned into a pbr322 vector in escherichia coli. deletion analysis of the recombinant plasmid showed that the pul coding sequence, probably with the regulator gene, was located entirely within a 4.2-kilobase segment derived from the chromosomal dna of k. aerogenes. e. coli cells carrying the recombinant plasmids produced about three- to sevenfold more pullulanase than did the wild-type strain of k. aerogenes w70. when the cloned cells of e. ...19853885852
in vitro studies on the antibacterial activities of ym-13115, a new broad-spectrum cephalosporin.the in vitro antibacterial activities of ym-13115, a new parenteral cephalosporin, were compared with those of ceftazidime, cefoperazone, and cefsulodin. the compound was highly active against the common members of the enterobacteriaceae and 2 to 256 times more active than cefoperazone. ym-13115 was as active as ceftazidime against citrobacter freundii, proteus vulgaris, and morganella morganii and two to four times more active than ceftazidime against escherichia coli, klebsiella pneumoniae, en ...19853890729
structure of wild-type and mutant repressors and of the control region of the rbt operon of klebsiella aerogenes.pentitol metabolism in klebsiella aerogenes is encoded by continuous ribitol (rbt) and d-arabitol (dal) operons transcribed in bipolar fashion and sandwiched between long stretches of homologous dna. the operons are separated by a central control region (2.2 kb) which encodes both the repressors and all the control sequences. the rbt repressor (270 amino acids) shows homology to the escherichia coli lac repressor and other dna-binding proteins. it is transcribed from the strand opposite the rbt ...19853891331
evolution of glutamine amidotransferase genes. nucleotide sequences of the paba genes from salmonella typhimurium, klebsiella aerogenes and serratia marcescens.the amide group of glutamine is a source of nitrogen in the biosynthesis of a variety of compounds. these reactions are catalyzed by a group of enzymes known as glutamine amidotransferases; two of these, the glutamine amidotransferase subunits of p-aminobenzoate synthase and anthranilate synthase have been studied in detail and have been shown to be structurally and functionally related. in some micro-organisms, p-aminobenzoate synthase and anthranilate synthase share a common glutamine amidotra ...19853894673
comparison of the regulatory regions of ilvgeda operons from several enteric organisms.the nucleotide sequence preceding the ilvgeda operon has been examined and compared in five enteric organisms. the sequence in escherichia coli b was identical to the earlier-described strain k-12 sequence. the sequences of salmonella typhimurium and klebsiella aerogenes were remarkably similar to that of e. coli and identical in that part of the leader region that specified the putative 32-amino-acid peptide. thus, identical secondary structures could be postulated for the leaders of all three ...19853900037
microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salts/glucose media; correlation of specific power and size of the atp pool.a parallel study has been made of the variation of the atp pool and the specific power of cells of klebsiella aerogenes during aerobic growth in glucose-limited medium under carefully defined conditions of growth and test. during the early part of exponential growth there was a marked increase in both the atp pool and the specific power to near constant values during the later stages of growth; oscillations about the mean values were observed for each parameter. with the exhaustion of glucose an ...19853903440
ribitol dehydrogenase of klebsiella aerogenes. sequence and properties of wild-type and mutant strains.evidence is presented for the sequence of 249 amino acids in ribitol dehydrogenase-a from klebsiella aerogenes. continuous culture on xylitol yields strains that superproduce 'wild-type' enzyme but mutations appear to have arisen in this process. other strains selected by such continuous culture produce enzymes with increased specific activity for xylitol but without loss of ribitol activity. one such enzyme, ribitol dehydrogenase-d, has pro-196 for gly-196. another, ribitol dehydrogenase-b, has ...19853904726
interspecific reconstitution of maltose transport and chemotaxis in escherichia coli with maltose-binding protein from various enteric bacteria.in escherichia coli, the periplasmic maltose-binding protein (mbp), the product of the male gene, is the primary recognition component of the transport system for maltose and maltodextrins. it is also the maltose chemoreceptor, in which capacity it interacts with the signal transducer tar (taxis to aspartate and some repellents). in studies of the maltose system in other members of the family enterobacteriaceae, we found that mbp is produced by salmonella typhimurium, klebsiella pneumoniae, ente ...19853905762
the functional significance of glucose dehydrogenase in klebsiella aerogenes.in order to assess the functional significance of the quinoprotein glucose dehydrogenase recently found to be present in k+ -limited klebsiella aerogenes, a broad study was made of the influence of specific environmental conditions on the cellular content of this enzyme. whereas high activities were manifest in cells from glucose containing chemostat cultures that were either potassium- or phosphate-limited, only low activities were apparent in cells from similar cultures that were either glucos ...19853907571
glutamate synthase from escherichia coli, klebsiella aerogenes, and saccharomyces cerevisiae. 19853911003
detoxification of mercury, cadmium, and lead in klebsiella aerogenes nctc 418 growing in continuous culture.klebsiella aerogenes nctc 418 growing in the presence of cadmium under glucose-, sulfate-, or phosphate-limited conditions in continuous culture exhibited sulfide formation and pi accumulation as the only demonstrable detoxification mechanisms. in the presence of mercury under similar conditions only hgs formation could be confirmed, by an increased sensitivity to mercury under sulfate-limited conditions, among others. the fact that the cells were most sensitive to cadmium under conditions of ph ...19853911898
microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salts/glucose media: growth in phosphate-limited medium.the power-time traces for cells of klebsiella aerogenes grown in phosphate-limited media were unlike those for cells grown in phosphate-sufficient media. at a phosphate concentration of 18 mumol dm-3 three phases of growth were recognized: the exponential growth phase during which phosphate became exhausted; a slower growth phase to the exhaustion of glucose, and a period of minimal growth when acetate, formed as secondary metabolite, was metabolized. at a concentration of 40 mumol dm-3 only two ...19853913845
growth of microorganisms in total parenteral nutrition mixtures and related clinical observations.the effect of lipid addition to tpn (total parenteral nutrition) solutions on microbial growth was investigated. staphylococcus epidermidis, which failed to grow or grew poorly in the absence of lipid, reached greater than 10(4) cfu/ml (colony forming units per ml), from an initial inoculum of approximately 50 cfu/ml after 24 h when lipid was added. candida albicans grew more slowly in the presence of lipid, but nevertheless reached 10(4) cfu/ml after 40 h incubation. klebsiella aerogenes grew r ...19853923044
growth of bacteria in enteral feeding solutions.solutions of clinifeed iso, triosorbon, vivonex standard (full- and half-strength) and vivonex hn were experimentally contaminated with two strains each of staphylococcus aureus, pseudomonas aeruginosa, klebsiella aerogenes, escherichia coli and enterobacter cloacae at concentrations of 10(2)-10(3) organisms/ml. samples were incubated at 4, 25 or 37 degrees c and viable counts were made at 0, 4, 8 and 24 h. no increase in numbers of any of the organisms was observed in any of the feeds during 24 ...19853927003
measuring the spermosphere colonizing capacity (spermosphere competence) of bacterial inoculants.spermosphere establishment by bacteria which were coated onto seeds was studied using soybean seeds treated with four bacterial strains at levels of log10 1 to 4 colony-forming units (cfu) per seed planted in a field soil mix, and incubated 48 h. each strain at every inoculum level developed spermosphere population densities of log10 4 to 8 cfu/seed, demonstrating an average multiplication of log10 3 cfu/seed. an alternative method was developed to differentially rank bacteria for spermosphere c ...19853933804
ofloxacin: in-vitro activity against clinical isolates.the in-vitro activity of ofloxacin, a new quinolone derivative, was evaluated in 273 bacterial strains isolated from urine. the results showed a remarkable activity against most gram-negative bacteria and, in particular, against enterobacter aerogenes, which is very difficult to eradicate even with third-generation cephalosporins.19853938347
microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salts/glucose media: inhibition by nalidixic acid.the addition of nalidixic acid (na) to exponentially growing cells of klebsiella aerogenes in simple salts/glucose media has a marked effect on the thermal and other growth parameters of the cells. mass and energy balances have been established for normal and inhibited growth. the decrease, with increasing na concentration, of energy allocated to biomass and the increase in that wasted is discussed in terms of the energy stored in macromolecular species by the cells under conditions of stress. t ...19853938832
phenotypic variations in cell envelope proteins of klebsiella aerogenes.the structure and functioning of the cell envelope of klebsiella aerogenes vary phenotypically with changes in the culture environment. these variations involve, in particular, the proteinaceous components. this contribution discusses results obtained in recent studies directed towards analysing the influence of growth conditions on membrane protein composition in this organism.19853940006
conservation of dna sequences for plasmid-mediated citrate utilization within the enterobacteria.southern blot dna-dna hybridization experiments with a cloned cit+ dna fragment as a probe showed that the plasmid-mediated cit+ determinants from four cit plasmids (r726, poh3001, poh3035, and poh30221) were all homologous. sequences homologous to the plasmid-borne cit+ gene were also found in total bacterial dna isolated from salmonella paratyphi b, salmonella enteritidis, salmonella typhimurium lt-2, citrobacter freundii, atcc 8090, citrobacter amalonaticus atcc 25405, klebsiella pneumoniae i ...19863941048
studies on microorganisms secured from different organs of slaughtered sheep with special reference to the microbial load in certain muscles.a total of 312 samples collected from different organs and muscles of dead, disabled, diseased, dying, and normal carcasses of sheep were examined bacteriologically to determine the different species of microorganisms as to their public health significance. in addition, the aerobic plate count (apc) and most probable number (mpn) coliform/g were determined of musculus biceps brachii, musculus quadriceps femoris, and musculus longissimus dorsi. the mean bacterial load was higher in summer than in ...19863964203
dynamics of resistance to cephalosporins.bacterial susceptibility to various cephalosporins of nine of the most frequently isolated organisms were analyzed for the five-year period 1980 to 1984. the disk diffusion susceptibility to cephalosporins of 1,640,031 strains of bacteria isolated from 242 hospitals in the united states were compared. no significant change has occurred in the susceptibility of escherichia coli, klebsiella pneumoniae, proteus mirabilis, haemophilus influenzae, or streptococcus faecalis for this period; enterobact ...19853986942
the emergence of resistance and the therapy of septicaemia.there were 658 positive blood cultures in st. thomas' hospital during 1982-1984, from which 301 enterobacteriaceae were isolated. most were of species that do not usually produce inducible class i beta-lactamases but 39 of the isolates were of species that do. many of these strains were resistant to the older cephalosporins but resistance to cefuroxime was uncommon and resistance to ceftazidime or cefotaxime was confined to five isolates of enterobacter cloacae (four of which were from patients ...19853986947
mineral nutrition of aerobacter aerogenes for valine production in a synthetic medium.the effect of a number of mineral salts, like dipotassium hydrogen phosphate, magnesium sulphate, and sodium chloride, and of some trace elements including iron, copper, cobalt, nickel, molybdenum, and calcium, on the production of valine by aerobacter aerogenes in a synthetic medium was investigated. it was found that all the mineral salts were necessary for valine formation. among the trace elements, iron and molybdenum were found to be necessary in minute concentrations for the optimum yield ...19854013530
biochemical changes during valine production by aerobacter aerogenes.the typical chemical changes taking place during valine production by a. aerogenes in a synthetic medium have been investigated. the principal carbon and nitrogen sources, sucrose and urea, respectively, have been found to be almost exhausted during an incubation period lasting 90 h. while the carbon compound was depleted right from the start of formentation, the disappearance of urea was noticeable after 16 h of incubation. the formation of valine in the broth started between 24 and 40 h of inc ...19854013531
crepitant cellulitis due to enterobacter aerogenes. 19854019153
activation of herpes simplex following dermabrasion. report of a patient successfully treated with intravenous acyclovir and brief review of the literature.herpes simplex labialis developed in a patient immediately following dermabrasion. the patient was hospitalized because the infection had spread rapidly over the dermabraded face and was complicated by secondary impetiginization. herpesvirus hominis type i and enterobacter aerogenes were isolated from cultures. intravenous acyclovir and oral antibiotics were administered. on this regimen, new vesicle formation ceased in 36 hours. complete resolution of the infection occurred within 6 days, with ...19854031143
treatment of gram-negative infections with aztreonam.twenty-one patients with serious gram-negative infections were treated with aztreonam. twenty of these were clinical and microbiologic cures; there was one clinical improvement with microbiologic persistence. no bacteria became resistant. cure rates were: bone and joint (11 of 11); skin and soft tissue (six of six); pneumonia (two of two); perinephric abscess (one of one); and intra-abdominal abscess (zero of one). the bacteria responsible for these infections included pseudomonas aeruginosa (12 ...19854038577
efficacy and safety of aztreonam-clindamycin versus tobramycin-clindamycin in the treatment of lower respiratory tract infections caused by aerobic gram-negative bacilli.a total of 80 patients were randomized to receive either aztreonam or tobramycin for the treatment of lower respiratory tract infections caused by gram-negative bacilli; all these patients received clindamycin concomitantly. a total of 53 patients were randomized to receive aztreonam-clindamycin; of these, 46 were clinically evaluable and 39 were bacteriologically evaluable. of the 46 clinically evaluable patients, 41 were considered cured, 3 failed to be cured, and 2 died during the study perio ...19854039118
liver abscess complicating crohn's disease presenting as thoracic empyema. case report and review of the literature.a 28-year-old man with a history of crohn's disease presented with right pleuritic pain and dyspnea. chest radiography showed a right pleural effusion. thoracocentesis yielded purulent fluid that subsequently grew enterobacter aerogenes. computed axial tomography of the abdomen revealed right subphrenic abscess and right hepatic lobe abscess. antibiotic therapy and surgical drainage resulted in complete recovery. a review of the english literature produced 18 cases of liver abscess complicating ...19854061480
a preliminary survey of aerobic bacteria in breast milk of mothers from the low-income group in nigeria.the aerobic bacteria colonizing breast milk of the low-income group in nigeria were quantified to assess its suitability for use in milk banks. in parallel, the nutritional and health status of donating mothers and their infants were assessed by physicians. the aerobic bacteria contained in the specimens included streptococcus salivarius, bacillus cereus, klebsiella pneumoniae, enterobacter aerogenes, streptococcus pneumoniae, streptococcus pyogenes, pseudomonas aeruginosa and staphylococcus epi ...19854071647
bacterial isolates from nsukka meat market: a zoonotic appraisal.swabs collected from butchers hands, knives, tables and meat displayed for sale at nsukka meat market showed that every item screened was contaminated. bacteria isolated include streptococcus faecalis, coagulase positive staphylococcus, clostridium perfringens, brucella species, corynebacterium haemolyticum, proteus mirabilis, p. vulgaris, escherichia coli, salmonella enteritidis, enterobacter aerogenes, pseudomonas multophilia, p. rubescens and beta-haemolytic streptococci. the zoonotic or publ ...19854077409
control of infection due to klebsiella aerogenes in a neurosurgical unit by withdrawal of all antibiotics. 19704099781
function of ubiquinone in electron transport from reduced nicotinamide adenine dinucleotide to nitrate and oxygen in aerobacter aerogenes.the possible role of quinones in the electron transport system of aerobacter aerogenes was investigated. the only quinone found in measurable amounts in bacteria grown in minimal media under both aerobic and anaerobic conditions was ubiquinone-8. membrane-bound ubiquinone-8 could be removed by extraction with pentane, or destroyed by ultraviolet irradiation, with a concomitant loss of both reduced nicotinamide adenine dinucleotide (nadh) oxidase and nadh-linked respiratory nitrate reductase acti ...19714100202
use of montmorillonite for the demonstration of capsules of klebsiella aerogenes and cornebacterium sp.a technique is described for the demonstration of bacterial capsules by using montmorillonite in combination with a conventional staining method.19714108650
enzyme evolution in the enterobacteriaceae.an immunological approach has been used for the study of alkaline phosphatase evolution in bacteria of the family enterobacteriaceae. antisera were prepared against alkaline phosphatase from escherichia coli and klebsiella aerogenes and tested against the unpurified alkaline phosphatases of 32 strains of enterobacteria by double diffusion and quantitative micro-complement fixation. the immunological relationships detected among the alkaline phosphatases of enterobacteria agree approximately with ...19724113124
diacetyl (acetoin) reductase from aerobacter aerogenes. kinetic mechanism and regulation by acetate of the reversible reduction of acetoin to 2,3-butanediol. 19734144829
the participation of cytochromes in the process of nitrate respiration in klesbsiella (aerobacter) aerogenes. 19734145134
effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in klebsiella aerogenes.at least two pathways exist in klebsiella aerogenes for glutamate synthesis. a mutant blocked in one pathway due to the loss of glutamate dehydrogenase (gltd) does not require glutamate and has the same growth characteristics as the parent strain in most media; however, its growth is inhibited by the analogues methionine sulfoximine and methionine sulfone. wild-type klebsiella is resistant to 0.1 m methionine sulfoximine or methionine sulfone, whereas the gltd mutant is sensitive to 1 mm concent ...19734145197
[biosynthesis of 4-aminobenzoic acid in aerobacter aerogenes]. 19724145580
glutamine synthetase and the regulation of histidase formation in klebsiella aerogenes. 19734145798
regulation of the synthesis of enzymes responsible for glutamate formation in klebsiella aerogenes. 19734146916
[biosynthesis of indole in not aerated cultures of klebsiella aerogenes (author's transl)]. 19734147736
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