Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| in vitro and in vivo antibacterial properties of fk 027, a new orally active cephem antibiotic. | fk 027 was more active than cefaclor, cephalexin, and amoxicillin against stock strains of a wide variety of gram-negative bacteria, including such opportunistic pathogens as citrobacter and enterobacter species and serratia marcescens. fk 027 was significantly more active than the three reference drugs against clinical isolates of escherichia coli, klebsiella pneumoniae, indole-positive and -negative proteus species, providencia species, haemophilus influenzae, and neisseria gonorrhoeae. it was ... | 1984 | 6561017 |
| enoxacin: worldwide in-vitro activity against 22451 clinical isolates. | a worldwide multicentre study involving 31 centres in eight countries was conducted to compare the in-vitro activity of enoxacin with that of other currently available antibiotics. enoxacin was active against virtually all of the species of enterobacteriaceae tested (mic90 less than or equal to 0.25-2 mg/l). it was also effective at inhibiting pseudomonas aeruginosa (mic90 = 2 mg/l; n = 3540), staphylococcus aureus and epidermidis (mic90 = 2 mg/l; n = 2635 and 837 resp.), acinetobacter calcoacet ... | 1984 | 6594322 |
| cefotetan: profile of in-vitro activity. | cefotetan, a novel 7 alpha-methoxy cephalosporin, was shown to be comparable to other third-generation cephalosporins when tested in vitro against 1063 gram-negative clinical isolates of 11 species. ninety per cent of all isolates of escherichia coli, klebsiella, proteus mirabilis, proteus vulgaris, proteus rettgeri, providencia stuartii, serratia marcescens and citrobacter freundii were inhibited by concentrations of cefotetan between 0.07 and 3.2 mg/l. activity against gram-positive cocci was ... | 1983 | 6601656 |
| in vitro antibacterial activity and beta-lactamase stability of e-0702, a new cephalosporin. | the in vitro activity of e-0702 was compared with the in vitro activity of cefotaxime, ceftazidime, moxalactam, and aztreonam against 600 gram-positive and gram-negative aerobic and anaerobic isolates. e-0702 had a minimal inhibitory concentration for 50% of isolates (mic50) of 25 micrograms for staphylococcus aureus, 50 micrograms for staphylococcus epidermidis, and 1.6 to 3.1 micrograms for streptococci, with streptococcus faecalis resistant. e-0702 had mic50s against escherichia coli, klebsie ... | 1983 | 6605718 |
| development of resistance to cephalosporins in clinical strains of citrobacter spp. | the predominant beta-lactam antibiogram of citrobacter freundii resembles that of enterobacter cloacae in demonstrating resistance to cephalothin and cefoxitin with susceptibility to the newer cephalosporins. four representative strains of c. freundii were reversibly induced to high-level beta-lactamase production by cefoxitin, and mutants with stable, high-level production were selected with cefamandole. the mutants were resistant to several second- and third-generation cephalosporins. comparis ... | 1984 | 6610388 |
| a new site-specific endodeoxyribonuclease from citrobacter freundii. | cfr10 i, a site-specific endonuclease from citrobacter freundii strain rfl10, was isolated. it recognizes and cleaves the family of related sequences: 5'pu decreases ccggpy to generate dna fragments with 5' tetranucleotide extensions. cfr10 i may be useful in molecular cloning experiments, especially in conjunction with other enzymes which generate the same terminal extensions. | 1983 | 6617873 |
| panophthalmitis and otitis interna in fire-bellied toads. | microbiologic and histologic studies were made of fire-bellied toads with signs of ocular and central nervous system disease. providencia alcalifaciens, citrobacter freundii, aeromonas hydrophila, and other gram-negative bacilli were isolated from the eyes and multiple tissues of ill toads. the histologic evaluations revealed severe panophthalmitis and otitis interna. | 1983 | 6643232 |
| [2 cases of osteomyelitis in acute leukemia in the induction phase of treatment]. | whereas children with acute leukemia are highly susceptible to infectious complications, the occurrence of acute osteomyelitis is extremely rare in these patients. the authors describe two such cases in children at onset of an acute lymphoblastic and of a myelomonocytic leukemia, respectively. in the former case, the clinical course has been characterized by the progressive involvement of several joints and bones. a citrobacter freundii was isolated in the synovial fluid of an involved knee. thi ... | 1983 | 6647082 |
| antibacterial activity of aztreonam: a synthetic monobactam. a comparative study with thirteen other antibiotics. | the in vitro activity of aztreoman (sq 26, 776), a new monocyclic beta-lactam antimicrobial agent, was determined against 1720 bacteria, all clinical isolates, and compared with that of thirteen beta-lactam and aminoglycoside antibiotics. aztreonam inhibited 90% of citrobacter diversus, citrobacter freundii, enterobacter agglomerans, e. coli, klebsiella pneumoniae, proteus mirabilis, proteus morganii, proteus rettgeri, proteus vulgaris and salmonella sp. by less than or equal to 0.4 micrograms m ... | 1983 | 6684718 |
| [immobilization of citrobacter freundii cells with tyrosine phenol-lyase activity by entrapment in natural gels]. | a comparative study on immobilization of citrobacter freundii cells by entrapment in carrageenan, agar, agar-agar, and gelatin gels (5, 10, and 15%) was carried out. gelatin gels were treated with glutaraldehyde to make them more rigid. as a result, the tyrosine phenol-lyase activity of these samples was less than that of free cells (about 40%). the yield of tp-lyase activity was 40--60% when cells were immobilized in 5% and 7% agar and agar-agar gels. the cells entrapped in carrageenan gels, th ... | 1984 | 6701163 |
| the distribution of repeating [gal beta 1,4glcnac beta 1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell lines bw5147 and phar 2.1. | the occurrence and distribution of the repeating disaccharide [gal beta 1,4glcnac beta 1,3] in the different types of asn-linked oligosaccharides in mouse lymphoma bw5147 cells have been studied. glycopeptides were prepared from cells grown in medium containing [6-3h]galactose, and the bi-, tri-, and tetraantennary asn-linked oligosaccharides were fractionated by serial lectin affinity chromatography on concanavalin a-sepharose, pea lectin -sepharose, leukoagglutinating phytohemagglutinin-agaros ... | 1984 | 6725252 |
| evolutionary divergence of the citrobacter freundii tryptophan operon regulatory region: comparison with other enteric bacteria. | the regulatory region of the trp operon of citrobacter freundii was sequenced and compared with the corresponding regions of other enteric bacteria. significant differences were noted in the promoter region. these differences are presumably responsible for the weak expression of the cloned trp operon in escherichia coli. the presumed operator region, although nonfunctional in e. coli, has dyad symmetry, but the sequence of the symmetrical region differs appreciably from those of operators that c ... | 1982 | 6749821 |
| characterization of indicator bacteria in municipal raw water, drinking water, and new main water samples. | municipal water samples were analyzed by membrane filter (mf) and presence-absence (p-a) tests for pollution indicator bacteria. in four years, 11 514 bacterial cultures were isolated from either raw water, drinking water, or new main water samples submitted to three environmental laboratories. the bacterial species occurring most often in all types of water samples were escherichia coli (11.6-39.7%), enterobacter aerogenes (18.1-26.3%), aeromonas hydrophila (8.8-17.0%), klebsiella pneumoniae (7 ... | 1982 | 6754044 |
| citrobacter infections in humans: experience at the seattle veterans administration medical center and a review of the literature. | the genus citrobacter is a distinct group of human pathogens comprising three species: citrobacter freundii (biotypes a and b), citrobacter amalonaticus, and citrobacter diversus. in this review the clinical and microbiologic experience during 1972-1978 at the seattle veterans administration medical center (seattle, wash.) with 298 isolates of citrobacter is analyzed in relation to a survey of the literature. the most common sources of citrobacter isolates were urine, sputum, and soft tissue exu ... | 1980 | 6763304 |
| isolation and characterization of an endo-beta-galactosidase from a new strain of escherichia freundii. | a new strain of escherichia freundii was isolated from human feces. compared with the previous strain (kitamikado, m., and ueno, r. (1970) bull. jpn. soc. sci. fish. 36, 1175-1180), this strain releases comparable levels of endo-beta-galactosidase with lower levels of exoglycosidases in the culture medium containing 0.3% of keratan sulfate. endo-beta-galactosidase was purified by an improved purification procedure involving amicon h1p10 hollow fiber filtration, qae-sephadex a-50, and cm-sephadex ... | 1980 | 6769923 |
| [taxonomic study of "citrobacter freundii" biochemical variants (author's transl)]. | biochemical variants (ornithine-decarboxylase-positive and beta-galactosidase-negative) of citrobacter freundii were characterized using numerical taxonomy, determination of deoxyribonucleic acid (dna) base composition and dna-dna hybridization. three clusters were detected by numerical taxonomy based on the assimilation of 146 carbohydrates. the atypical strains were dispersed into two clusters along with typical c. freundii strains. furthermore, m-hydroxybenzoate metabolism, determination of b ... | 1980 | 6770734 |
| tk polyagglutination produced in vitro by an endo-beta-galactosidase. | after treatment with the culture supernatant of bacteroides fragilis or with an endo-beta-galactosidase from escherichia freundii, the human red blood cells are tk-activated, i.e. agglutinable by bs ii lectin and their blood group i and i activities are reduced. these results suggest that b. fragilis produces an endo-beta-galactosidase activity and that the main antigenic determinant of tk polyagglutinable red cells is a terminal n-acetyl-glucosamine residue. | 1980 | 6773244 |
| isolation and structural characterization of human lymphocyte and neutrophil gangliosides. | gangliosides were isolated from purified preparations of human peripheral blood lymphocytes and neutrophils. structural analyses and comparisons were performed by direct probe mass spectrometry and by degradation studies with the following enzymes: escherichia freundii endo-beta-galactosidase; clostridium perfringens and arthrobacter ureafaciens neuraminidase; and jack bean beta-n-acetylhexosaminidase and beta-galactosidase. this combination of techniques allowed us to obtain carbohydrate compos ... | 1981 | 6780555 |
| purification and characterization of endo-beta-galactosidase from escherichia freundii induced by hog gastric mucin. | a new procedure for inducing and purifying endo-beta-galactosidase from escherichia freundii was described. the enzyme was found to be induced with high efficiency in culture medium containing smith-degraded hog gastric mucin, which was prepared from a commercially available starting material. endo-beta-galactosidase was then purified by ammonium sulfate fractionation, deae-sephadex chromatography, and affinity chromatography on sepharose conjugated with the smith-degraded mucin. the enzyme thus ... | 1981 | 6783649 |
| cell-free biosynthesis of erythroglycan in a microsomal fraction from k-562 cells. | particulate membrane preparations from k-562 [human cml (chronic-myelogenous-leukaemia)-derived] cells catalyse the transfer of [3h]galactose from udp-[3h]-galactose and [3h]n-acetylglucosamine from udp-[3h]n-acetylglucosamine into an endogenous product that on digestion with pronase yields long-chain glycopeptides (mol.wt. 7000--10 000) called 'erythroglycan'. incorporation of either labelled sugar increased up to 60 min of incubation time. the labelled erythroglycan was isolated by chromatogra ... | 1981 | 6798962 |
| biochemical properties of the high-molecular-weight glycopeptides released from the cell surface of human teratocarcinoma cells. | the present study deals with the biochemical properties of high-molecular-weight glycopeptides isolated from the surface of human teratocarcinoma cells. this cell surface material released by mild trypsin digestion from galactose-labeled human teratocarcinoma cells, tera i and pa1, was digested extensively with pronase. most of the resulting glycopeptides were large and were excluded from a sephadex g-50 column. the properties of these large cell surface glycopeptides isolated from tera i cells ... | 1982 | 6802482 |
| [effect of oxygen and substrates for growth on the superoxide dismutase and catalase activity of microorganisms]. | the activity of superoxide dismutase (sod) and catalase in azotobacter vinelandii, citrobacter freundii, rhodopseudomonas capsulata, thiocapsa roseopersicina and spirulina platensis is far higher when the cultures are grown under the aerobic conditions. the activities of sod and catalase are higher in r. capsulata cells cultivated in a medium with glucose in the dark under the aerobic conditions than in cells grown under the same conditions but in the light. r. capsulata grown in a medium with g ... | 1982 | 6803110 |
| cell-associated glycosaminoglycans of human teratocarcinoma-derived cells of line pa 1. | human teratocarcinoma-derived cells of line pa 1, which are capable of differentiating in vitro [zeuthen, j. et al. (1980) int j. cancer, 25, 19-32], incorporate label from radioactive sulfate and/or glucosamine into several large-sized glycosaminoglycans including hyaluronate, chondroitin sulfate/dermatan sulfate co-polymers, heparan sulfate and keratan-sulfate-like molecules. all these polysaccharide fractions were identified by specific degradation methods. the labeled hyaluronate was degrade ... | 1982 | 6804234 |
| endo-beta-galactosidase from escherichia freundii. | 1982 | 6808309 | |
| in vitro activity of new cephalosporins against multi-resistant gram-negative bacteria. | the in vitro sensitivities of 47 multi-resistant gram-negative bacilli against six third generation cephalosporins were investigated. overall, moxalactam was the most active compound and cefoperazone the least active. ceftizoxime and ceftazidime were very active against enterobacteriaceae. ceftriaxone and ceftizoxime were active against escherichia coli and klebsiella spp., but were inactive against pseudomonas spp.; achromobacter sp. was resistant to all drugs, while citrobacter freundii was se ... | 1983 | 6832840 |
| the in-vitro activity of o-demethylfortimicin. | the activity of o-demethylfortimicin against ampicillin, cefazolin and carbenicillin resistant bacteria was determined. o-demethylfortimicin showed excellent in-vitro activity against escherichia coli, klebsiella pneumoniae, enterobacter cloacae, ent. agglomerans, ent. aerogenes, citrobacter freundii, proteus vulgaris, morganella, and providencia inhibiting these organisms at less than or equal to 3.1 mg/l. it was particularly effective against ent. cloacae and serratia marcescens that were resi ... | 1983 | 6833173 |
| bacteriological studies of the enteric flora of patients treated with bicozamycin (cgp 3543/e) for acute nonparasitic diarrhea. | during a therapeutic trial of bicozamycin (bi) for traveler's diarrhea, aerobically grown, gram-negative bacteria, predominantly escherichia coli, were decreased by 2 to 3 logs per g of stool; the number of bi-resistant gram-negative bacteria did not increase. resistant species were most often citrobacter freundii, klebsiella pneumoniae, and morganella morganii, and few bi-resistant e. coli strains were isolated. cross-resistance between bi and other antimicrobial agents was not found. resistanc ... | 1983 | 6859842 |
| presence of erythroglycan on human k-562 chronic myelogenous leukemia-derived cells. | total glycopeptides from human k-562 cells, labeled metabolically with [3h]glucosamine or [3h]mannose, were prepared by extracting the cells with organic solvents to remove lipids and by digesting the residue with pronase. 3h-labeled glycopeptides were fractionated on sephadex g-50 revealing a high molecular weight fraction (mr = 7,000 to 11,000), comprising approximately 10% of the [3h]glucosamine and 25% of the [3h]mannose label. digestion of this glycopeptide fraction with endo-beta-galactosi ... | 1980 | 6928849 |
| cefmenoxime (sce-1365), a novel broad-spectrum cephalosporin: in vitro and in vivo antibacterial activities. | the activity of cefmenoxime (sce-1365), 7 beta-[2-(2-aminothiazol-4-yl)-(z)-2-methoxyiminoacetamido]-3-[(1-methyl-1h-tetrazol-5-yl)thiomethyl]ceph-3-em-4-carboxylic acid, was compared with that of other cephalosporins. cefmenoxime exhibited high activity against a wide variety of gram-positive and gram-negative bacteria. the in vitro activity of cefmenoxime against streptococcus pyogenes, haemophilus influenzae, and enterobacteriaceae, including indole-positive proteus, serratia marcescens, ente ... | 1981 | 6941742 |
| neutral glycosphingolipids of human acute leukemias. | neutral glycosphingolipids were isolated from the malignant cells of several patients with different types of acute leukemia. analyses were performed by high performance liquid chromatography combined with enzymatic hydrolysis of glycosphingolipids using glycosidases (escherichia freundii endo-beta-galactosidase, jack bean beta-galactosidase, and beef kidney beta-hexosaminidase). we found that acute leukemia cells contain very little or none of the more complex neutral glycosphingolipids that ar ... | 1982 | 6955304 |
| rhodanese activity: a simple and reliable taxonomic tool for gram-negative bacteria. | the thiosulphate: cyanide sulphurtransferase (rhodanese) test of vandenbergh, bawdon and berk (1979) has been simplified and 2469 strains from a wide variety of sources representing different biochemical, serological or phage-pattern entities were tested. the percentages of rhondanese-producing strains were: escherichia coli 98%, shigella flexneri serovars 1-5%, x and y 0%, other shigellae 73-100%, yersinia spp. 0%, salmonella subgenera i-iv 0%, citrobacter freundi 16%, klebsiella 37%, enterobac ... | 1982 | 6958873 |
| effect of a combination of benzylpenicillin or ampicillin and dicloxacillin on peptidoglycan synthesis in a cell-free enzyme system from a beta-lactamase producing strain of citrobacter freundii. | the membrane fraction prepared from beta-lactamase producing citrobacter freundii gn346 catalyzed in vitro peptidoglycan synthesis from uridine-5'-diphosphate-n-acetylmuramyl-l-alanyl-d-glutamyl-meso-diaminopimelyl- d-alanyl-d-alanine and uridine-5'-diphosphate-n-acetylglucosamine, which was accompanied by the release of alanine from the carboxyl terminal end of the former substrate. though this reaction was inhibited by benzylpenicillin (pcg) and ampicillin (abpc), the reaction was relatively i ... | 1980 | 6967868 |
| purification and properties of chromosomally mediated beta-lactamase from citrobacter freundii gn7391. | both a penicillinase and a cephalosporinase are present in a strain of citrobacter freundii (gn7391) resistant to beta-lactam antibiotics. the penicillinase was identical to the type ia penicillinases (type iii by richmond classification), mediated by rms212 and r-tem. a cephalosporinase, typical of enterobacteriaceae chromosomal beta-lactamase (type i by richmond classification), was purified from the strain. it gave a single protein band on polyacrylamide gel electrophoresis and immunoelectrop ... | 1980 | 6973612 |
| in vitro activity of apalcillin compared with that of other new penicillins and anti-pseudomonas cephalosporins. | apalcillin, a naphthydridine derivative of ampicillin, was compared with ticarcillin, azlocillin, mezlocillin, piperacillin, cefotaxime, and cefoperazone against gram-negative and gram-positive bacterial isolates and with cefsulodin and tobramycin against pseudomonas aeruginosa. the minimal concentrations of apalcillin at which 50 and 90% of hospital isolates of escherichia coli were inhibited were similar to those of mezlocillin and piperacillin (1.6 and 100 micrograms/ml, respectively). apalci ... | 1982 | 6981375 |
| beta-lactamase inhibitory activities and synergistic effects of 5,6-cis-carbapenem antibiotics. | twelve 5,6-cis-carbapenem antibiotics were examined for their beta-lactamase inhibitory activities, their types of inhibitions, and their synergistic activities with other beta-lactam antibiotics. all the carbapenems inhibited eight types of beta-lactamases including cephalosporinases which were insensitive to clavulanic acid and sulbactam. the sulfonyloxy ethyl carbapenems were the most active inhibitors; they inhibited all beta-lactamases in a progressive fashion, whereas some of the hydroxyl ... | 1982 | 6982889 |
| screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. | a total of 393 clinical bacterial isolates were tested for their ability to agglutinate yeast cells of either saccharomyces cerevisiae or candida albicans. a positive agglutination of yeasts that could be prevented by methyl alpha-d-mannoside was taken as an indication for the possible presence of a mannose-specific lectin (carbohydrate-binding protein) on the surface of the tested bacteria. agglutination tests on glass slides showed that 38% of all the isolates tested were positive in their cap ... | 1980 | 6989854 |
| cefoxitin resistance by a chromosomal cephalosporinase in escherichia coli. | cefoxitin resistance, an unique property found in clinical isolates of escherichia coli was investigated. cefoxitin resistant strains, 255 and gn206, produced cephalosporinase constitutively. the cephalosporinase was located in the periplasm, and its production was considered to be mediated by chromosomal gene(s). cephalosporinase-less mutants from both strains were susceptible to cefoxitin as well as other beta-lactam antibiotics, suggesting that the cephalosporinase was responsible for the res ... | 1980 | 7002896 |
| regulation of hydrogenase activity in enterobacteria. | proteus vulgaris, escherichia coli, and citrobacter freundii cells were devoid of hydrogenase activity when grown on complex medium or minimal medium plus glucose in the presence of saturating levels of dissolved oxygen. anaerobically grown cells had appreciable hydrogenase activity. cells grown anaerobically in the presence of co (an inhibitor of hydrogenase) or nitrate (an electron acceptor) lacked hydrogenase activity. to make hydrogenase essential for anaerobic growth, cells were grown on fu ... | 1980 | 7002905 |
| biochemical characteristics and identification of enterobacteriaceae isolated from meats. | the isolation and identification of 2,220 enterobacteriaceae from meats indicated that escherichia coli biotype i, enterobacter agglomerans, and serratia liquefaciens were the principal types to be differentiated in meats. citrobacter freundii, klebsiella pneumoniae, enterobacter cloacae, and enterobacter hafniae were also commonly identified. identification of isolates by the encise ii (roche diagnostics inc., nutley, n.j.) and minitek (bbl microbiology systems, cockeysville, md.) coding system ... | 1981 | 7013705 |
| coliform species recovered from untreated surface water and drinking water by the membrane filter, standard, and modified most-probable-number techniques. | the species of total coliform bacteria isolated from drinking water and untreated surface water by the membrane filter (mf), the standard most-probable-number (s-mpn), and modified most-probable-number (m-mpn) techniques were compared. each coliform detection technique selected for a different profile of coliform species from both types of water samples. the mf technique indicated that citrobacter freundii was the most common coliform species in water samples. however, the fermentation tube tech ... | 1981 | 7013706 |
| influence of diluents, media, and membrane filters on detection fo injured waterborne coliform bacteria. | pure cultures of escherichia coli, klebsiella pneumoniae, enterobacter aerogenes, and citrobacter freundii were injured ( greater than 90%) in water from a dead-end section of the bozeman, montana, distribution system. the effects of the following laboratory variables on the enumeration efficiency of injured and undamaged control cells were examined: (i) diluent composition, temperature, and time of exposure; (ii) media, using various formulations employed in enumerating gram-negative bacteria; ... | 1982 | 7034648 |
| [quantitative bacterial ecology of normal nasal mucosa]. | a quantitative research into the aerobic bacteria of human nasal cavities has been carried out; 183 healthy individuals observed, negative results 18 (9.83%). streptococcus, enterococcus, staphylococcus aureus, staphylococcus epidermidis, micrococcus, streptococcus pneumoniae, neisseria, were numerically determined and the incidence of each single species or genus exactly specified. among gram-negative bacteria, enterobacter, providencia, proteus, citrobacter freundii, haemophilus influenzae, kl ... | 1981 | 7034653 |
| the inhibition of bacterial cell growth by ketone bodies. | the effect of ketone bodies on the growth, in culture, of escherichia coli was investigated. both growth and glucose utilisation were inhibited in the presence of 20 mmol/l d-3-hydroxybutyrate. lower concentrations of d-3-hydroxybutyrate caused proportionally less inhibition of growth. acetoacetate also inhibited growth but other glycolytic inhibitors and chemical analogues of d-3-hydroxybutyrate either did not inhibit or proved to be too toxic for bacterial growth. citrate enhanced the ketone b ... | 1981 | 7034705 |
| sensitivity, specificity, and reproducibility of the automicrobic system (with the enterobacteriaceae-plus biochemical card) for identifying clinical isolates of gram- negative bacilli. | two independent laboratories tested 1,743 clinical isolates by using the enterobacteriaceae-plus biochemical card in the automicrobic system (ams) and identical standard reference methods. included were 55 isolates representing 11 species that cannot be identified by the enterobacteriaceae-plus biochemical card computer program; 3 or these isolates were incorrectly identified as pseudomonas cepacia. with the other 1,688 isolates, 5% of the ams identifications were considered to be equivocal (pro ... | 1982 | 7040457 |
| exclusive localization of colicin a in cell cytoplasm of producing bacteria. | the production of colicin a in citrobacter freundii and in escherichia coli was studied. after induction with low concentrations of mitomycin c, these organisms differed with regards to cell growth, cell viability, and kinetics of colicin a biosynthesis. despite these differences, immunoferritin labelling on ultra-thin sections of induced frozen cells demonstrated that colicin a was located exclusively within the cell cytoplasm in both types of bacteria. by using protein markers, it was shown th ... | 1981 | 7042335 |
| rapid differentiation of bacterial meningitides by direct gas-liquid chromatography. | rapid identification of haemophilus influenzae and other bacillary meningitides was attempted by gas-liquid chromatography (glc) of the metabolic by-products in broth cultures and in cerebrospinal fluid (csf) samples obtained from experimental meningitis produced in new zealand white male rabbits. these results were correlated with the glc of csf of meningitis patients. a major peak with retention time of succinic acid was found in the broth cultures of all bacilli tested including h. influenzae ... | 1982 | 7042759 |
| [cytochrome composition and content of citrobacter freundii]. | citrobacter freundii, strain 62, produces considerable amounts of cytochromes c and b when it grows in media with different carbon compounds under both aerobic anc anaerobic conditions. the content of cytochromes c increases under anaerobic conditions, particularly in the presence of formate or dmso, and under aerobic conditions if formate is added. in contrast, the amount of cytochromes b decreased when the culture is grown in media with formate under anaerobic conditions. cytochrome o is princ ... | 1982 | 7045598 |
| the microbiology of transmissible murine colonic hyperplasia. | the relationship of citrobacter freundii (4280) to the development of colonic hyperplasia in mice was investigated. the number of citrobacter freundii (4280) in the descending colon were quantitated at intervals following experimental inoculation, and findings were correlated with previously reported histological and ultrastructural findings. citrobacter freundii (4280) was a transient infection, lasting 2-3 weeks, after which time mice no longer harbored the organism. neomycin sulfate and tetra ... | 1980 | 7052371 |
| structures of branched blood group a-active glycosphingolipids in human erythrocytes and polymorphism of a- and h-glycolipids in a1 and a2 subgroups. | the structures of two branched variants of blood group a-active glycolipid, ac and ad (hakomori, s., stellner, k., and watanabe, k. (1972) biochem. biophys. res. commun. 49, 1061-1068), isolated from human type a-erythrocytes, were elucidated. the intact glycolipids and fragments produced by treatment with endo-beta-galactosidase from escherichia freundii (fukuda, m. n., and matsumura, g. (1976) j. biol. chem. 251, 6218-6225) were analyzed by methylation. in addition, a specific limited enzyme d ... | 1982 | 7053380 |
| pulmonary toxicity of endotoxins: comparison of lipopolysaccharides from various bacterial species. | lipopolysaccharides from three gram-negative bacteria isolated from bale cotton and piggery air were analyzed for their chemical composition, and their pulmonary toxicity for guinea pigs, lethal toxicity for mice, and pyrogenicity for rabbits were measured. lipopolysaccharides from enterobacter agglomerans and citrobacter freundii had closely related chemical compositions; both were pyrogenic for rabbits and caused a dose-dependent influx of polymorphonuclear leukocytes into the airways of guine ... | 1982 | 7056574 |
| recovery of heat-injured citrobacter freundii cells. | 1982 | 7068522 | |
| a new glycolipid antigen isolated from human erythrocyte membranes reacting with antibodies directed to globo-n-tetraosylceramide (globoside). | two glycolipid fractions were separated by high performance liquid chromatography from human erythrocyte membranes and which were reactive with antibodies to globo-n-tetraosylceramide (globoside). the reactivity to the antibody was abolished by treatment with endo-beta-galactosidase of escherichia freundii which specifically hydrolyzes lacto-n-glycosyl series glycolipids, but does not hydrolyze globo series glycolipids. one of the fractions was isolated by repeated high performance liquid chroma ... | 1982 | 7068643 |
| synthesis of the branched form of erythroglycan by friend gm979 erythroleukemic cells. | mouse gm979 erythroleukemic cells were found to synthesize the branched form of erythroglycan, a large polylactosamine structure known to be attached to band 3 and possibly to other proteins on human erythrocytes. total protein-derived oligosaccharides from gm979 cells, labeled metabolically with [3h]glucosamine, [3h]mannose, or [3h]galactose, were prepared by hydrazinolysis after extracting the lipids. the 3h-labeled oligosaccharides were fractionated on sephadex g-50 revealing 10-25% of each l ... | 1982 | 7085669 |
| hand carriage of aerobic gram-negative rods by health care personnel. | a quantitative culture technique (hand washed in a glove containing broth for 30 s) was used to determine the frequency of hand carriage of aerobic gram-negative rods by various groups of health care workers and 104 control subjects. overall, 31% of health care workers carried aerobic gram-negative rods on their hands compared to 59% of control subjects (p < 0.001). enterobacter agglomerans accounted for 40% of the isolates, and other enterobacter spp. 7%. other organisms included acinetobacter ... | 1982 | 7097001 |
| partial structural analysis of concanavalin a binding glycopeptides of large size from human teratocarcinoma-derived cells: cleavage by hydrazinolysis and alkaline borohydride. | pronase digests of cultured teratocarcinoma-derived cells (pa 1) of human origin have been previously shown to contain large-sized glycopeptides (relative mass (mr) greater then 7400), of which 15-23% are retained by columns of concanavalin a (con a) - sepharose and can be eluted with 10 mm methyl alpha-d-mannopyranoside. the present data show that this fraction (a - con a ii) contains a family of glycopeptides that are degradable with anhydrous hydrazine as well as with 0.05 m naoh - 1 m nabh4. ... | 1982 | 7116211 |
| cell and atp yields of citrobacter freundii growing with fumarate and h2 or formate in continuous culture. | out of 19 strains belonging to the family enterobacteriaceae only escherichia coli and citrobacter strains fermented fumarate exclusively to succinate. this fermentation was dependent on the presence of molecular hydrogen or formate. the inability of these micro-organisms to convert fumarate to succinate, acetate and co2 correlated with their lack, or low activity, of oxaloacetate decarboxylase. continuous culture experiments were performed with citrobacter freundii in minimal or complex medium ... | 1982 | 7142962 |
| underestimation of total-coliform counts by the membrane filter verification procedure. | coliforms, primarily citrobacter freundii, gave negative verification results in the total-coliform membrane filtration test. the organisms produced gas from lactose in brilliant green bile broth but not in lauryl tryptose broth. the discrepancy was related to the peptone sources used in the media. | 1982 | 7149709 |
| studies on the oa-6129 group of antibiotics, new carbapenem compounds. ii. in vitro evaluation. | the oa-6129 group of antibiotics, new carbapenem compounds, had relatively potent antimicrobial activities against gram-positive and gram-negative bacteria. synergism of compound oa-6129a in combination with conventional beta-lactam antibiotics was observed in antimicrobial activity against beta-lactamase producers such as proteus vulgaris gn 76 and citrobacter freundii gn 346. the new carbapenem compounds were slightly superior to ps-5 in stability to kidney homogenates of various animal specie ... | 1982 | 7174514 |
| bacterial colonization of the upper intestine in mild tropical malabsorption. | the bacterial flora of the upper intestine has been examined in symptomatic expatriate adults with mild tropical malabsorption, without steatorrhoea, persisting for many months after return to a western environment. seven of the 11 patients had enterobacteria in luminal fluid or mucosal samples in numbers ranging from 10(3) to 10(8) per ml or per g. the most common isolate was klebsiella pneumoniae (in four cases); citrobacter freundii, serratia marcescens and pseudomonas spp. were also detected ... | 1980 | 7210130 |
| an outbreak of diarrhea due to citrobacter freundii in a neonatal special care nursery. | 1980 | 7216341 | |
| growth conditions and heat resistance of citrobacter freundii. | the influence of the growth medium and the growth temperature on the heat resistance of citrobacter freundii has been established. logarithmic growth phase cells grown on rich media have a higher heat resistance than cells of the same phase grown on minimal media. this finding was independent of type of carbon source in the growth medium, but the kind of carbon source has a definite influence on the heat resistance. logarithmic phase cells grown at 37 degrees c are much more heat stable than cel ... | 1980 | 7235690 |
| plasmid specification of resistance to antibacterial compounds in environmental citrobacter freundii. | citrobacter freundii from an environmental source displayed resistance to a wide variety of antibacterials. some of these resistances could be transferred by conjugation with frequencies as high as 10(-2). the complexity of plasmid involvement in the antibacterial resistance of this species was demonstrated by isolation of plasmid dna from two isolates. in one strain, resistance to beta-lactam antibiotics was specified by a 55 x 10(6) molecular weight plasmid, whereas kanamycin and neomycin resi ... | 1981 | 7237280 |
| relationship of colonic mucosal background to neoplastic proliferative activity in dimethylhydrazine-treated mice. | proliferative activity of background and neoplastic colonic mucosa was examined following five months of weekly injections of 1,2-dimethylhydrazine (20 mg/kg) and one or four months of rest to determine whether previously reported changes may result from an acute or chronic effect of dimethylhydrazine and whether differences exist between stages of neoplasia. to determine whether neoplasia is responsive to a proliferative stimulus, 1,2-dimethylhydrazine dihydrochloride-treated mice were inoculat ... | 1981 | 7248933 |
| in vitro synergism of fr-31564, a new phosphonic acid antibiotic. | against most test strains of gram-negative bacilli, the in vitro effect of fr-31564 together with beta-lactam antibiotics or trimethoprim was strongly synergistic; with tetracycline and nalidixic acid the effect was additive; and with gentamicin and sulfamethoxazole the effect was additive or antagonistic. fr-31564 was markedly synergistic with beta-lactam antibiotics against beta-lactam antibiotic-resistant gram-negative bacilli such as klebsiella pneumoniae, enterobacter aerogenes, e. cloacae, ... | 1981 | 7287590 |
| [citrobacter freundii interaction with epithelial cells and macrophages in vitro]. | electron-microscopic study has revealed the capacity of enteropathogenic c. freundii strain to adhere to the glycocalyx of epithelial hela cells, to proliferate on their surface and to induce the toxic damage of their organelles. c. freundii, possessing a fibrillar microcapsule, have been shown to be capable of resisting the action of the lysosome enzymes of guinea-pig macrophages and disorganizing macrophage cultures. the latter fact should be taken into consideration in working with c. freundi ... | 1981 | 7293573 |
| effect of clindamycin prophylaxis on the colonic microflora in patients undergoing colorectal surgery. | clindamycin was given intravenously to 15 patients undergoing colorectal surgery in an initial dose of 600 mg, given at induction of anesthesia followed by 6 doses of 600 mg at 8-h intervals. series of serum samples and fecal specimens were taken for analysis of clindamycin concentrations. tissue samples from the gut wall were taken at surgery. the highest serum concentrations observed occurred 30 min after administration of clindamycin and varied between 6.8 and 37.9 microgram/ml (mean, 14.8 +/ ... | 1981 | 7325640 |
| a biotype of enterobacteriaceae intermediate between citrobacter and enterobacter. | the authors isolated two strains of an unnamed bacterial biotype with characteristics intermediate between those of enterobacter and citrobacter. the organisms did not produce acetyl-methyl carbinol, but decarboxylated lysine. apart from the latter trait, they most closely resemble h2s-negative citrobacter freundii. they differ biochemically from all other currently accepted species of enterobacteriaceae. their pathogenic significance appears similar to that of the two genera they most closely r ... | 1980 | 7352413 |
| in vitro and in vivo antibacterial activities of fr-31564, a new phosphonic acid antibiotic. | fr-31564, a new phosphonic acid antibiotic, was active against most gram-negative bacteria except serratia marcescens and glucose-nonfermenting gram-negative rods excluding pseudomonas aeruginosa. the antibacterial activity in vitro of fr-31564 was stronger than that of fosfomycin especially against escherichia coli, klebsiella pneumoniae, enterobacter species and p. aeruginosa. fr-31564 also was active against gram-negative bacteria resistant to beta-lactam antibiotics and against gentamicin-re ... | 1980 | 7372548 |
| bacterial contamination of drinking water supplies in a modern rural neighborhood. | on six occasions during a 15-month period, the private well and spring water supplies in a modern rural neighborhood of 78 households were examined for total coliforms, fecal coliforms, staphylococcus aureus, and standard plate count bacteria. more than one-third of the water supplies were unsatisfactory on at least one occasion as judged by standard plate counts over 10(3)/ml and the presence of coliforms, fecal coliforms, and/or s. aureus. citrobacter freundii, klebsiella pneumoniae, and esche ... | 1980 | 7377773 |
| [isolation, purification and some properties of tyrosine-phenol-lyase from citrobacter freundii cells]. | a new procedure for isolation of tyrosine-phenol-lyase from the cells of c. freundii strain 62 allowing to obtain a highly purified enzyme with a high yield at a reduced time expenditure has been developed. the procedure described differs from the well-known method for isolation of the enzyme from the cells of escherichia intermedia and erwinia herbicola. some properties of the enzyme from c. freundii 62, e.g. stability, dependence of the enzyme activity on some mono- and bivalent cations and ph ... | 1980 | 7378508 |
| [conditions for the formation of molecular hydrogen by citrobacter freundii]. | cell suspensions of citrobacter freundii grown under anaerobic and aerobic conditions are capable of evolving molecular hydrogen. hydrogen evolution is more active by the cells of anaerobic cultures in the exponential growth phase in the presence of reduced methylviologen or formate. the optimal temperature for hydrogen evolution from formate is about 40 degrees c, the optimal ph is 6.2 to 6.4. the presence of o2 in the gaseous phase inhibits hydrogen evolution by the cells; however the inhibiti ... | 1980 | 7412615 |
| evaluation of a new assay for vi antibody in chronic carriers of salmonella typhi. | the assay for serum antibody to the salmonella typhi capsular polysaccharide (vi) antigen has recently been revised because of the availability of a purified, highly polymerized vi antigen. we compared this revised vi antibody assay to the traditional one for potential usefulness in the surveillance of chronic enteric carriers of s. typhi. the purified vi antigen of citrobacter freundii was incorporated into a passive hemagglutination assay for serum vi antibody; the standard vi antibody assay w ... | 1980 | 7419698 |
| agglutinins to bacteria in crohn's disease. | sera from patients with crohn's disease were tested for antibodies against organisms which are thought to cause inflammatory bowel disease in animals, or have been implicated in human crohn's disease. control sera were collected from healthy individuals and patients with ulcerative colitis. sera from crohn's disease and controls failed to agglutinate clostridium colinum or campylobacter sputorum subsp. mucosalis and two strains of mycobacterium paratuberculosis (m26 and m27). most of the sera ag ... | 1980 | 7429299 |
| nosocomial bacteriuria: a prospective study of case clustering and antimicrobial resistance. | to investigate the role of cross-infection in nonepidemic nosocomial bacteriuria in a large, university-affiliated hospital, we identified in adult patients admitted over an 11-week period all cases caused by organisms of the same genus, species, and antimicrobial susceptibility and clustered by date of onset and hospital ward. further laboratory studies were conducted to verify clustering. among the 3452 patients studied, 194 cases of nosocomial bacteriuria were identified; 49 appeared clustere ... | 1980 | 7436158 |
| modification of early dimethylhydrazine carcinogenesis by colonic mucosal hyperplasia. | the interaction of colonic mucosal hyperplasia with early 1,2-dimethylhydrazine (dmh) carcinogenesis was studied in random-bred nih swiss mice utilizing hyperplasia-inducing citrobacter freundii. mice inoculated with this bacterium developed significantly more dmh focal atypia than did mice without hyperplasia following a single dose of dmh (20 mg/kg). mice with hyperplasia also developed dmh focal atypia with diminished doses of dmh (10 and 5 mg/kg), while normal mice did not. the effect of c. ... | 1980 | 7438077 |
| effect of glucose on the biochemical properties of the bacterial cytoplasmic membrane. | the cytoplasmic membrane isolated from cells of citrobacter freundii growing in a cultivation medium with glucose was found to exhibit a decreased ability to oxidize formate and succinate and a decreased activity of formate, succinate and lactate dehydrogenase as compared with the cytoplasmic membrane of cells growing on galactose. the activation energy for dehydrogenation and oxidation of succinate simultaneously increases. the quantitative content of cytochromes and quinones in both types of m ... | 1980 | 7439843 |
| [citrobacter freundii osteomyelitis of the mandible: review of the literature and report of a case]. | 1980 | 7471661 | |
| the 3'-terminal half of the btub gene of citrobacter freundii defines essential residues and supports a topological model for the outer membrane receptor. | 1995 | 7476175 | |
| isolation and sequence analysis of rpoh genes encoding sigma 32 homologs from gram negative bacteria: conserved mrna and protein segments for heat shock regulation. | the rpoh genes encoding homologs of escherichia coli sigma 32 (heat shock sigma factor) were isolated and sequenced from five gram negative proteobacteria (gamma or alpha subgroup): enterobacter cloacae (gamma), serratia marcescens (gamma), proteus mirabilis (gamma), agrobacterium tumefaciens (alpha) and zymomonas mobilis (alpha). comparison of these and three known genes from e.coli (gamma), citrobacter freundii (gamma) and pseudomonas aeruginosa (gamma) revealed marked similarities that should ... | 1995 | 7501460 |
| the structure of the citrobacter freundii o8a,8b o-specific polysaccharide containing d-xylofuranose. | 1994 | 7528641 | |
| site-directed mutagenesis of tyrosine-71 to phenylalanine in citrobacter freundii tyrosine phenol-lyase: evidence for dual roles of tyrosine-71 as a general acid catalyst in the reaction mechanism and in cofactor binding. | tyr71 is an invariant residue in all known sequences of tyrosine phenol-lyase (tpl). the substitution of tyr71 in tpl by phenylalanine results in a mutant y71f tpl with no detectable activity (greater than 3 x 10(5)-fold reduction) for beta-elimination of l-tyrosine. y71f tpl can react with s-alkylcysteines, but these substrates exhibit kcat values reduced by 10(3)-10(4)-fold, while the kcat/km values are reduced by 10(2)-10(3)-fold, compared to wild-type tpl. however, for substrates with good l ... | 1995 | 7547970 |
| in vitro antibacterial activities of fk037: a new parenteral cephalosporin. | the antibacterial and bactericidal activities and the stability of fk037 to beta-lactamases were investigated and compared with those of cefpirome, flomoxef, ceftazidime, ceftizoxime, and vancomycin. against clinical isolates of methicillin-sensitive and methicillin-resistant staphylococcus aureus, fk037 was less active than vancomycin but more potent than the other drugs tested. among highly methicillin-resistant staphylococci (mic for methicillin: 200 mg/l or higher), none of the strains were ... | 1995 | 7555206 |
| genetic and biochemical characterization of citrobacter rodentium sp. nov. | an unusual bacterial pathogen of laboratory mice has been previously classified as an atypical biotype of citrobacter freundii. designated c. freundii biotype 4280, this bacterium is the etiologic agent of transmissible murine clonic hyperplasia. an eaea gene has been shown to be present in this organism and to be necessary for virulence in laboratory mice. however, other biotypes of c. freundii lack dna homology with the eaea gene. because of the recent reclassification in which five named spec ... | 1995 | 7559949 |
| ceftibuten: a review of antimicrobial activity, spectrum and other microbiologic features. | ceftibuten is a new, orally administered cephalosporin with exceptional beta-lactamase stability and potency against commonly isolated gram-negative pathogens. more than 90% of recent enterobacteriaceae clinical isolates were inhibited by < or = 8 micrograms/ml of ceftibuten. in only five enteric species (citrobacter freundii, enterobacter aerogenes, enterobacter cloacae, morganella morganii, serratia marcescens) were more than 15% of strains resistant (minimal inhibitory concentrations (mic, wi ... | 1995 | 7567314 |
| taxonomic diversity of anaerobic glycerol dissimilation in the enterobacteriaceae. | a total of 1,123 strains representing 128 taxa in the enterobacteriaceae (named species or subspecies and genomic species) were screened for the presence of glycerol dehydrogenases and 1,3-propanediol dehydrogenase. only eight taxa, citrobacter freundii sensu stricto, c. youngae, c. braakii, c. werkmanii, citrobacter genomospecies 10 and 11, enterobacter gergoviae and klebsiella pneumoniae subsp. pneumoniae could grow fermentatively on glycerol and possessed both glycerol dehydrogenase type i (i ... | 1995 | 7569322 |
| a comparison of immunomagnetic separation plus enrichment with conventional salmonella culture in the examination of raw sausages. | immunomagnetic separation with additional enrichment was used in conjunction with improved selective media to improve the isolation of salmonellae from raw sausages. the isolation rate achieved was almost double that of conventional culture with no increase in processing time. the selective media gave an overall specificity of approximately 74%; all false-positive pickoffs being identified as citrobacter freundii. it is believed that this method represents a significant advance in the isolation ... | 1995 | 7576516 |
| phosphatase production and activity in citrobacter freundii and a naturally occurring, heavy-metal-accumulating citrobacter sp. | the ability of a naturally occurring citrobacter sp. to accumulate cadmium has been attributed to cellular precipitation of cdhpo4, utilizing hpo4(2-) liberated via the activity of an overproduced, cd-resistant acid-type phosphatase. phosphatase production and heavy metal accumulation by batch cultures of this strain (n14) and a phosphatase-deficient mutant were compared with two reference strains of citrobacter freundii. only strain n14 expressed a high level of acid phosphatase and accumulated ... | 1995 | 7582003 |
| effects of tazobactam on the frequency of the emergence of resistant strains from enterobacter cloacae, citrobacter freundii, and proteus vulgaris (beta-lactamase derepressed mutants). | when enterobacter cloacae, citrobacter freundii, and proteus vulgaris were treated with piperacillin (pipc) in combination with tazobactam (taz), the in vitro frequency of emergence of resistant strains (beta-lactamase producing mutants) was lower than with pipc or ceftazidime (caz) treated bacteria. in a mouse intraperitoneal infection model caused by e. cloacae, beta-lactamase derepressed mutants were detected following therapy with pipc or caz, although no derepressed mutants were detected af ... | 1995 | 7592048 |
| multicentre comparative study on the antibacterial activity of fk-037, a new parenteral cephalosporin. | the in vitro antibacterial activity of fk-037, a new parenteral cephalosporin structurally related to cefpirome and cefepime, was compared with that of cefotaxime, ceftazidime, aztreonam, cefpirome, cefepime, imipenem and meropenem against 1,837 clinical isolates obtained from three spanish hospitals. fk-037 inhibited 90% of enterobacteriaceae isolates at < or = 0.25 microgram/ml, with the exception of enterobacter aerogenes (mic90 1 microgram/ml), enterobacter cloacae and citrobacter freundii ( ... | 1995 | 7614970 |
| comparative evaluation of orally active antibiotics against community-acquired pathogens: a multi-center study in five mediterranean countries. | in 5 mediterranean countries 7902 pathogens, all isolated in 1992 and 1993 from community-acquired infections, were studied for susceptibility to the following orally active antibiotics: penicillin g, ampicillin, ampicillin + sulbactam, amoxycillin + clavulanic acid (both 2:1 ratio), cefalexin, cefaclor, cefuroxime, cefetamet, doxycycline and erythromycin. ten centers in italy, 4 centers in greece, 3 centers in spain, and 1 center in lebanon and saudi arabia contributed to this study; all center ... | 1995 | 7629552 |
| biochemical and molecular characterization of the oxidative branch of glycerol utilization by citrobacter freundii. | glycerol dehydrogenase (ec 1.1.1.6) and dihydroxyacetone kinase (ec 2.7.1.29) were purified from citrobacter freundii. the dehydrogenase is a hexamer of a polypeptide of 43,000 da. the enzyme exhibited a rather broad substrate specificity, but glycerol was the preferred substrate in the physiological direction. the apparent kms of the enzyme for glycerol and nad+ were 1.27 mm and 57 microm, respectively. the kinase is a dimer of a polypeptide of 57,000 da. the enzyme was highly specific for the ... | 1995 | 7635824 |
| national survey of the in vitro spectrum of piperacillin-tazobactam tested against more than 40,000 aerobic clinical isolates from 236 medical centers. | hospital microbiology laboratories from 41 states participated in a bacterial antimicrobial susceptibility study comparing in vitro results generated by the standardized disk diffusion method. over 41,000 freshly isolated aerobic and facultative strains, representing all specimen types (except stools and urines), were tested for their susceptibility to piperacillin-tazobactam and 21 other antimicrobial agents. enterococcus spp. was the second or third most common isolate from intraabdominal, gyn ... | 1995 | 7648835 |
| isolation of a citrobacter freundii strain which carries the escherichia coli o157 antigen. | a biochemically typical strain of citrobacter freundii which carries the escherichia coli o157 antigen is described. the significance of differentiating such strains from typical e. coli o157 strains is stressed. | 1993 | 7681442 |
| structure and antigenic properties of citrobacter freundii lipopolysaccharides. | citrobacter freundii strain e69366 was detected in a colony immunoassay with a rabbit antiserum prepared to a strain of escherichia coli belonging to serogroup o157. lipopolysaccharide (lps) was shown to contain the epitope(s) involved in antibody binding. lps prepared from strain e69366, with hot phenol contained core-lps that migrated to the water-phase and long-chain lps that separated into the phenol-phase. a total of 36 strains of cit. freundii were analysed for their lps profiles by sds-pa ... | 1993 | 7683640 |
| gcva, a lysr-type transcriptional regulator protein, activates expression of the cloned citrobacter freundii ampc beta-lactamase gene in escherichia coli: cross-talk between dna-binding proteins. | escherichia coli jrg582 is an ampd ampe deletion derivative of strain hfrh and accordingly it is derepressed for expression of the cloned inducible beta-lactamase gene of citrobacter freundii, carried on plasmid pnu305. following chemical mutagenesis of jrg582(pnu305) a cefotaxime sensitive mutant was isolated, cs51(pnu305), which produced low levels of beta-lactamase due to a mutation in the host chromosome. two recombinant plasmids containing genomic dna from e. coli hfrh, namely pub5608 and p ... | 1995 | 7704273 |
| purification of 1,3-propanediol dehydrogenase from citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in escherichia coli. | 1,3-propanediol dehydrogenase (ec 1.1.1.202) was purified to homogeneity from citrobacter freundii grown anaerobically on glycerol in continuous culture. the enzyme is an octamer of a polypeptide of 43,400 da. when tested as a dehydrogenase, the enzyme was most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. in the physiological direction, 3-hydroxypropionaldehyde was the preferred substrate. the apparent km values of the enzyme for 3-hydroxypro ... | 1995 | 7721705 |
| induction of beta-lactamase by cefoxitin in anaerobic intestinal microflora. | beta-lactamases produced by two anaerobic bacterial strains, bacteroides ovatus ax34:1 and clostridium butyricum nbl3, were shown to be significantly inducible under anaerobic conditions in subinhibitory concentrations of cefoxitin. the induction ratio of beta-lactamase production for bacteroides ovatus was 2.6 and for clostridium butyricum 1.6. incubation of faecal samples with different concentrations of cefoxitin did not result in any induction of beta-lactamase production. when adding a high ... | 1995 | 7729448 |
| production of a plasmid mediated ampc-like beta-lactamase by a klebsiella pneumoniae septicaemia isolate. | a septicaemia klebsiella pneumoniae isolate from greece (ath1) was shown to be resistant to third generation cephalosporins, aztreonam and cefoxitin and this resistance was not decreased in the presence of clavulanic acid. the gene coding for the resistance phenotype, associated with a beta-lactamase showing cephalosporinase activity and a pi of 8.6, could be transferred into escherichia coli k-12 by conjugation and transformation. dna-hybridisation showed that this gene was located on two diffe ... | 1994 | 7730237 |
| structural analysis of the sialylated n- and o-linked carbohydrate chains of recombinant human erythropoietin expressed in chinese hamster ovary cells. sialylation patterns and branch location of dimeric n-acetyllactosamine units. | the n-linked carbohydrate chains of recombinant human erythropoietin expressed in cho cells were quantitatively released with peptide-n4-(n-acetyl-beta-glucosaminyl)asparagine amidase f, separated from the remaining o-glycoprotein by gel-permeation chromatography, and subsequently fractionated via fplc on mono q, hplc on lichrosorb-nh2 and high-ph anion-exchange chromatography on carbopac pa1. the purified sialylated oligosaccharides were analyzed by one-dimensional and two-dimensional 500-mhz 1 ... | 1995 | 7737204 |
| site-directed mutagenesis of his343-->ala in citrobacter freundii tyrosine phenol-lyase. effects on the kinetic mechanism and rate-determining step. | his343 in citrobacter freundii tyrosine phenol-lyase is conserved in all known sequences of both tyrosine phenol-lyase and tryptophan indole-lyase; it is located near the active-site lys257 in c. freundii tyrosine phenol-lyase [antson, a. a., demidkina, t. v., gollnick, p., danter, z., von tersch, r.l., long, j., berezhnoy, s. n., phillips, r. s., harutyunyan, e. h. & wilson, k. s. (1993) biochemistry 32, 4195--4206]. in order to evaluate the role of his343 in the reaction mechanism of tyrosine ... | 1995 | 7744078 |