Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (phaz2) in ralstonia eutropha h16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase. | an intracellular 3-hydroxybutyrate (3hb)-oligomer hydrolase (phaz2(reu)) of ralstonia eutropha was purified from escherichia coli harboring a plasmid containing phaz2(reu). the purified enzyme hydrolyzed linear and cyclic 3hb-oligomers. although it did not degrade crystalline poly(3-hydroxybutyrate) (phb), the purified enzyme degraded artificial amorphous phb at a rate similar to that of the previously identified intracellular phb (iphb) depolymerase (phaz1(reu)). the enzyme appeared to be an en ... | 2003 | 12775684 |
| detection and enumeration of aromatic oxygenase genes by multiplex and real-time pcr. | our abilities to detect and enumerate pollutant-biodegrading microorganisms in the environment are rapidly advancing with the development of molecular genetic techniques. techniques based on multiplex and real-time pcr amplification of aromatic oxygenase genes were developed to detect and quantify aromatic catabolic pathways, respectively. pcr primer sets were identified for the large subunits of aromatic oxygenases from alignments of known gene sequences and tested with genetically well-charact ... | 2003 | 12788736 |
| nitric oxide reductase (norb) genes from pure cultures and environmental samples. | a pcr-based approach was developed to recover nitric oxide (no) reductase (norb) genes as a functional marker gene for denitrifying bacteria. norb database sequences grouped in two very distinct branches. one encodes the quinol-oxidizing single-subunit class (qnorb), while the other class is a cytochrome bc-type complex (cnorb). the latter oxidizes cytochrome c, and the gene is localized adjacent to norc. while both norb types occur in denitrifying strains, the qnorb type was also found in a var ... | 2003 | 12788753 |
| molecular diversity of denitrifying genes in continental margin sediments within the oxygen-deficient zone off the pacific coast of mexico. | to understand the composition and structure of denitrifying communities in the oxygen-deficient zone off the pacific coast of mexico, the molecular diversity of nir genes from sediments obtained at four stations was examined by using a pcr-based cloning approach. a total of 50 operational taxonomic units (otus) for nirk and 82 otus for nirs were obtained from all samples. forty-four of the nirs clones and 31 of the nirk clones were sequenced; the levels of similarity of the nirs clones were 52 t ... | 2003 | 12788762 |
| phylogeny and characterization of three nifh-homologous genes from paenibacillus azotofixans. | in this paper, we report the cloning and characterization of three paenibacillus azotofixans dna regions containing genes involved in nitrogen fixation. sequencing analysis revealed the presence of nifb1h1d1k1 gene organization in the 4,607-bp saci dna fragment. this is the first report of linkage of a nifb open reading frame upstream of the structural nif genes. the second (nifb2h2) and third (nifh3) nif homologues are confined within the 6,350-bp hindiii and 2,840-bp ecori dna fragments, respe ... | 2003 | 12788777 |
| controlled clinical comparison of bact/alert standard aerobic medium with bactec standard aerobic medium for culturing blood. | standard aerobic media are widely used for culturing blood with the bact/alert (biomérieux, inc., durham, n.c.) (bm) and bactec 9240 (bd diagnostic systems, sparks, md.) (bd) automated continuously monitoring instrument systems. although similarly composed of soybean-casein digest broths, the formulations of the standard aerobic media available for these instruments differ from each other in supplements and in sodium polyanetholesulfonate concentration. therefore, we compared the standard aerobi ... | 2003 | 12791854 |
| a homology model of penicillin acylase from alcaligenes faecalis and in silico evaluation of its selectivity. | a three-dimensional model of the relatively unknown penicillin acylase from alcaligenes faecalis (pa-af) was built up by means of homology modeling based on three different crystal structures of penicillin acylase from various sources. an in silico selectivity study was performed to compare this homology model to the structure of the escherichia coli enzyme (pa-ec) in order to find any selectivity differences between the two enzymes. the program grid was applied in combination with the principal ... | 2003 | 12851931 |
| phylogenetic analysis of bacterial and archaeal arsc gene sequences suggests an ancient, common origin for arsenate reductase. | the ars gene system provides arsenic resistance for a variety of microorganisms and can be chromosomal or plasmid-borne. the arsc gene, which codes for an arsenate reductase is essential for arsenate resistance and transforms arsenate into arsenite, which is extruded from the cell. a survey of genbank shows that arsc appears to be phylogenetically widespread both in organisms with known arsenic resistance and those organisms that have been sequenced as part of whole genome projects. | 2003 | 12877744 |
| bioreactor cultivation of escherichia coli for production of recombinant penicillin g amidase from alcaligenes faecalis. | the penicillin g amidase (pga) from alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in escherichia coli. the corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of pga was induced by repeated addition of the inducer rhamnose, that served also ... | 2003 | 12882560 |
| control of metalloprotein reduction potential: compensation phenomena in the reduction thermodynamics of blue copper proteins. | the reduction thermodynamics (delta h degrees '(rc) and delta s degrees '(rc)) for native paracoccus versutus amicyanin, for alcaligenes faecalis s-6 pseudoazurin, and for the g45p, m64e, and k27c variants of pseudomonas aeruginosa azurin were measured electrochemically. comparison with the data available for other native and mutated blue copper proteins indicates that the features of metal coordination and the electrostatic potential due to the protein matrix and the solvent control the reducti ... | 2003 | 12885256 |
| cloning of a nitrilase gene from the cyanobacterium synechocystis sp. strain pcc6803 and heterologous expression and characterization of the encoded protein. | the gene encoding a putative nitrilase was identified in the genome sequence of the photosynthetic cyanobacterium synechocystis sp. strain pcc6803. the gene was amplified by pcr and cloned into an expression vector. the encoded protein was heterologously expressed in the native form and as a his-tagged protein in escherichia coli, and the recombinant strains were shown to convert benzonitrile to benzoate. the active enzyme was purified to homogeneity and shown by gel filtration to consist probab ... | 2003 | 12902216 |
| nitrogen fixation genetics and regulation in a pseudomonas stutzeri strain associated with rice. | the pseudomonas stutzeri strain a1501 (formerly known as alcaligenes faecalis) fixes nitrogen under microaerobic conditions in the free-living state and colonizes rice endophytically. the authors characterized a region in strain a1501, corresponding to most of the nif genes and the rnf genes, involved in electron transport to nitrogenase in rhodobacter capsulatus. the region contained three groups of genes arranged in the same order as in azotobacter vinelandii: (1) nifb fdx orf3 nifq orf5 orf6; ... | 2003 | 12904565 |
| isolation of strains belonging to the cosmopolitan polynucleobacter necessarius cluster from freshwater habitats located in three climatic zones. | more than 40 bacterial strains belonging to the cosmopolitan polynucleobacter necessarius cluster (betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. sequences affiliated with the freshwater p. necessarius cluster are among the most frequently detected in studies on bacterial diversity in freshwater ecosystems. despite this frequent detection with culture-independent techniques and the cosmopolitan occurrence of members affiliated with ... | 2003 | 12957910 |
| [suppurative meningitis in infant caused by alcaligenes faecalis]. | 1952 | 12987217 | |
| alcaligenes faecalis meningitis complicated by hydrocephalus in a premature twin; report of case; intramuscular chloramphenicol therapy. | 1952 | 12995997 | |
| differentiation of alcaligenes faecalis from brucella bronchisepticus by biochemical and nutritional methods. | 1953 | 13034718 | |
| [a case of mixed meningitis caused by alcaligenes faecalis and flavobacterium arborescens]. | 1953 | 13073497 | |
| nitrogen compounds utilized by alcaligenes faecalis. | 1953 | 13096506 | |
| [a case of alcaligenes faecalis septicemia]. | 1953 | 13101207 | |
| phosphorylation coupled to electron transport in cell-free extracts of alcaligenes faecalis. | 1953 | 13117884 | |
| the nomenclatural type of the genus deleya and the consequences of deleya aesta and alcaligenes aquamarinus being synonyms. | the genus deleya was created to encompass a number of marine organisms that had previously been classified in diverse genera. deleya aesta was designated as the type species of the genus. subsequent work indicated that deleya aesta, alcaligenes aquamarinus and alcaligenes faecalis subsp. homari were heterotypic synonyms. consequently, akagawa & yamasato (int j syst bacteriol 39, 462-466, 1989) concluded that, based on rules 23a and 51b of the bacteriological code (1975 revision), the oldest legi ... | 2003 | 13130071 |
| d-erythrose metabolism in a strain of alcaligenes faecalis. | 1956 | 13345750 | |
| [case of puerperal sepsis caused by alcaligenes faecalis]. | 1956 | 13384634 | |
| [haemophilus bronchisepticus and alcaligenes faecalis. i. some differential characters]. | 1955 | 13403279 | |
| [haemophilus bronchisepticus and alcaligenes faecalis. ii. sensitivity to antibiotics]. | 1955 | 13403280 | |
| potassium uptake by alcaligenes faecalis. | 1957 | 13426108 | |
| degradation of ergothioneine by alcaligenes faecalis. | 1957 | 13475253 | |
| [intestinal infections by alcaligenes faecalis & their analogy with intestinal syndromes due to moraxella lwoffi]. | 1958 | 13534033 | |
| [typhic syndrome & alcaligenes faecalis]. | 1958 | 13586108 | |
| nucleic acid synthesis in penicillin-treated alcaligenes faecalis. | 1958 | 13610807 | |
| alcaligenes faecalis infection, its incidence in calcutta. | 1958 | 13611275 | |
| is alcaligenes faecalis a pathogen, contaminant or normal intestinal flora. | 1958 | 13620310 | |
| alcaligenes faecalis; its systematic study. | 1959 | 13630541 | |
| [inoculation of alcaligenes faecalis]. | 1959 | 13660437 | |
| disposition of membranes in alcaligenes faecalis. | 1960 | 13688633 | |
| degradation of ergothioneine by cell-free extracts of alcaligenes faecalis. | 1961 | 13752370 | |
| factors affecting the formation of gum by alcaligenes faecalis. | 1960 | 13765189 | |
| properties of gums formed by alcaligenes faecalis. | 1961 | 13765190 | |
| a case of measles encephalitis complicated by alcaligenes faecalis septicemia. | 1960 | 13769431 | |
| [apropos of alcaligenes faecalis]. | 1961 | 13776262 | |
| [on differentiation of bordetella (b.) bronchiseptica with special reference to differentiation from alcaligenes faecalis]. | 1960 | 13848986 | |
| participation of an rna fraction in peptide synthesis in the presence of a purified enzyme system from alcaligenes faecalis. | 1962 | 13866780 | |
| further experiments on the potassium uptake by alcaligenes faecalis. | 1962 | 13906379 | |
| [a study of the saccharolytic properties of alcaligenes faecalis]. | 1962 | 13917568 | |
| the metabolism of isoxanthopterin by alcaligenes faecalis. | 1963 | 13932252 | |
| degradation of ergothioneine by cell-free extracts of alcaligenes faecalis. ii. production of glutamic acid. | booth, james s. (university of southern california, los angeles) and milo d. appleman. degradation of ergothioneine by cell-free extracts of alcaligenes faecalis. ii. production of glutamic acid. j. bacteriol. 85:654-657. 1963.-on the basis of oxidation and paper chromatographic procedures, glutamic acid was identified as the end product of ergothioneine degradation by cell-free extracts of alcaligenes faecalis. hydrogen sulfide and ammonia yields were determined. several differences between the ... | 1963 | 14042946 |
| medium for differentiating the gram-negative, nonfermenting bacilli of medical interest. | sellers, walter (u.s. air force school of aerospace medicine, brooks air force base, tex.). medium for differentiating the gram-negative, nonfermenting bacilli of medical interest. j. bacteriol. 87:46-48. 1964.-an agar-slant medium is described for differentiating gram-negative, nonfermenting bacilli of medical interest. differences in the ability of bacilli to grow anaerobically in the presence of nitrate, to produce ph changes, to produce n(2) from nitrite and nitrate (singly and in combinatio ... | 1964 | 14102872 |
| amino acid code in alcaligenes faecalis. | amino acid incorporation into protein, promoted by synthetic polynucleotides, was studied in a cell-free extract of alcaligenes faecalis (combined guanine and cytosine content of dna, 66 percent). in the limited survey, 18 code triplets were found to specify the amino acids as in escherichia coli (combined guanine and-cytosine content of dna, 52 percent). at least six of the eight possible triplets containing adenine and uracil were meaningful. no exceptions to the universality of the amino acid ... | 1964 | 14108299 |
| b. alcaligenes faecalis septicemia in the newborn. | 1964 | 14119395 | |
| agar electrophoresis of colicines with an alcaligenes faecalis indicator strain. | 1964 | 14156322 | |
| antibiotics of alcaligenes faecalis. | 1964 | 14197404 | |
| the metabolism of xanthine-8-carboxylic acid by alcaligenes faecalis. | 1964 | 14245395 | |
| some comparative biochemical studies of alcaligenes faecalis and agrobacterium radiobacter. | 1955 | 14353822 | |
| [case of chronic eczema caused by bacterium coli, enterococcus and alcaligenes faecalis]. | 1954 | 14361225 | |
| organisms resembling alcaligenes faecalis. | 1960 | 14423858 | |
| alcaligenes faecalis infection in the newborn. | 1960 | 14445883 | |
| rearing of germfree and monocontaminated chicks in rigid plastic isolators. | a plexiglas isolator for rearing germfree vertebrates is described. plastic components were precut and finished by a plexiglas supplier. this equipment is relatively inexpensive; moreover, neither special facilities nor skills are required in their construction. advantages of this type of isolator are: readily dismantled for cleaning, durable, excellent visibility of the interior, and minimal maintenance. in 30 separate groups of animals, 27 of these yielded germfree chicks. many of these chicks ... | 1962 | 14486221 |
| multiplex real-time pcr method to identify shiga toxin genes stx1 and stx2 and escherichia coli o157:h7/h- serotype. | a multiplex real-time pcr method to simultaneously detect the stx1 and stx2 genes of shiga toxin-producing escherichia coli and a unique conserved single-nucleotide polymorphism in the e. coli o157:h7/h- uida gene has been developed. there is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. the pcr efficiencies were >/= 1.89, and as few as 6 cfu/reaction could be detected. | 2003 | 14532101 |
| extended-spectrum beta-lactamases in klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of shv- and ctx-m-type beta-lactamases. | a huge variety of extended-spectrum beta-lactamases (esbls) have been detected during the last 20 years. the majority of these have been of the tem or shv lineage. we have assessed esbls occurring among a collection of 455 bloodstream isolates of klebsiella pneumoniae, collected from 12 hospitals in seven countries. multiple beta-lactamases were produced by isolates with phenotypic evidence of esbl production (mean of 2.7 beta-lactamases per isolate; range, 1 to 5). shv-type esbls were the most ... | 2003 | 14576117 |
| molecular characterization and substrate preference of a polycyclic aromatic hydrocarbon dioxygenase from cycloclasticus sp. strain a5. | cycloclasticus sp. strain a5 is able to grow with petroleum polycyclic aromatic hydrocarbons (pahs), including unsubstituted and substituted naphthalenes, dibenzothiophenes, phenanthrenes, and fluorenes. a set of genes responsible for the degradation of petroleum pahs was isolated by using the ability of the organism to oxidize indole to indigo. this 10.5-kb dna fragment was sequenced and found to contain 10 open reading frames (orfs). seven orfs showed homology to previously characterized genes ... | 2003 | 14602629 |
| diversity and succession of the intestinal bacterial community of the maturing broiler chicken. | the diversity of bacterial floras in the ilea and ceca of chickens that were fed a vegetarian corn-soy broiler diet devoid of feed additives was examined by analysis of 1,230 partial 16s rrna gene sequences. nearly 70% of sequences from the ileum were related to those of lactobacillus, with the majority of the rest being related to clostridiaceae (11%), streptococcus (6.5%), and enterococcus (6.5%). in contrast, clostridiaceae-related sequences (65%) were the most abundant group detected in the ... | 2003 | 14602645 |
| development and application of a dapb-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium pseudomonas stutzeri a15. | pseudomonas stutzeri a15 is a nitrogen-fixing bacterium isolated from paddy rice. strain a15 is able to colonize and infect rice roots. this strain may provide rice plants with fixed nitrogen and hence promote plant growth. in this article, we describe the use of dapb-based in vivo expression technology to identify p. stutzeri a15 genes that are specifically induced during colonization and infection (cii). we focused on the identification of p. stutzeri a15 genes that are switched on during rice ... | 2003 | 14602651 |
| fragments of pro-peptide activate mature penicillin amidase of alcaligenes faecalis. | penicillin amidase from alcaligenes faecalis is a recently identified n-terminal nucleophile hydrolase, which possesses the highest specificity constant (kcat/km) for the hydrolysis of benzylpenicillin compared with penicillin amidases from other sources. similar to the escherichia coli penicillin amidase, the a. faecalis penicillin amidase is maturated in vivo from an inactive precursor into the catalytically active enzyme, containing one tightly bound ca2+ ion, via a complex post-translational ... | 2003 | 14622260 |
| achromobacter insolitus sp. nov. and achromobacter spanius sp. nov., from human clinical samples. | a polyphasic taxonomic study (employing whole-cell protein and fatty acid analyses, 16s rdna sequencing, dna-dna hybridization, determination of dna g+c content, antibiotic susceptibility testing and extensive phenotypic characterization) was performed on 10 isolates that appeared to be related to alcaligenes faecalis. the isolates were recovered from diverse environments that included human clinical samples. 16s rdna sequence analysis indicated that these isolates belonged to the genus achromob ... | 2003 | 14657110 |
| kerstersia gyiorum gen. nov., sp. nov., a novel alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of alcaligenes denitrificans rüger and tan 1983 as achromobacter denitrificans comb. nov. | a polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. phenotypically, these isolates resembled alcaligenes faecalis. whole-cell protein analysis distinguished two different species, and this was confirmed by dna-dna hybridizations. cellular fatty acid analysis and 16s rdna sequence analysis indicated that these isolates were related to the genera alcaligenes, bordetella, achromobacter and pigmentiphaga and belonged to the family alcaligenaceae ... | 2003 | 14657111 |
| pcr typing of genetic determinants for metallo-beta-lactamases and integrases carried by gram-negative bacteria isolated in japan, with focus on the class 3 integron. | from january 2001 to december 2002, 587 strains of gram-negative bacterial isolates demonstrating resistance to ceftazidime and a combination of sulbactam and cefoperazone were subjected to a disk diffusion screening test using sodium mercaptoacetic acid; 431 strains (73.4%) appeared to produce metallo-beta-lactamase (mbl). of these 431 strains, 357 were found by pcr to carry genes for imp-1 type mbl (bla(imp-1)), while only 7 and 67 strains carried the imp-2 gene (bla(imp-2)) and the vim-2 gene ... | 2003 | 14662918 |
| nitrous oxide reductase (nosz) gene fragments differ between native and cultivated michigan soils. | the effect of standard agricultural management on the genetic heterogeneity of nitrous oxide reductase (nosz) fragments from denitrifying prokaryotes in native and cultivated soil was explored. thirty-six soil cores were composited from each of the two soil management conditions. nosz gene fragments were amplified from triplicate samples, and pcr products were cloned and screened by restriction fragment length polymorphism (rflp). the total nosz rflp profiles increased in similarity with soil sa ... | 2004 | 14711656 |
| the functional role of the binuclear metal center in d-aminoacylase: one-metal activation and second-metal attenuation. | our structural comparison of the tim barrel metal-dependent hydrolase(-like) superfamily suggests a classification of their divergent active sites into four types: alphabeta-binuclear, alpha-mononuclear, beta-mononuclear, and metal-independent subsets. the d-aminoacylase from alcaligenes faecalis da1 belongs to the beta-mononuclear subset due to the fact that the catalytically essential zn(2+) is tightly bound at the beta site with coordination by cys(96), his(220), and his(250), even though it ... | 2004 | 14736882 |
| electrochemical studies of arsenite oxidase: an unusual example of a highly cooperative two-electron molybdenum center. | arsenite oxidase from alcaligenes faecalis, an unusual molybdoenzyme that does not exhibit a mo(v) epr signal during oxidative-reductive titrations, has been investigated by protein film voltammetry. a film of the enzyme on a pyrolytic graphite edge electrode produces a sharp two-electron signal associated with reversible reduction of the oxidized mo(vi) molybdenum center to mo(iv). that reduction or oxidation of the active site occurs without accumulation of mo(v) is consistent with the failure ... | 2004 | 14769044 |
| little known tropical diseases. | 1949 | 14771478 | |
| red-leg in tree-frogs caused by bacterium alkaligenes. | 1950 | 14778948 | |
| bacterium alcaligenes (alcaligenes faecalis) infections in man. | 1951 | 14827072 | |
| [acute meningitis due to alcaligenes faecalis in newborn]. | 1951 | 14830508 | |
| alcaligenes faecalis meningitis and bacteremia concurrent with bacillary dysentery; report of a case with necropsy. | 1952 | 14902735 | |
| molybdenum-containing arsenite oxidase of the chemolithoautotrophic arsenite oxidizer nt-26. | the chemolithoautotroph nt-26 oxidizes arsenite to arsenate by using a periplasmic arsenite oxidase. purification and preliminary characterization of the enzyme revealed that it (i) contains two heterologous subunits, aroa (98 kda) and arob (14 kda); (ii) has a native molecular mass of 219 kda, suggesting an alpha2beta2 configuration; and (iii) contains two molybdenum and 9 or 10 iron atoms per alpha2beta2 unit. the genes that encode the enzyme have been cloned and sequenced. sequence analyses r ... | 2004 | 14996791 |
| l-alanine auxotrophy of lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches. | using a chemically defined medium without l-alanine, lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. a comparative genetic analysis showed that all known genes involved in l-alanine biosynthesis are absent from the genome of l. johnsonii. this auxotrophy was complemented by heterologous expression of the bacillus subtilis l-alanine dehydrogenase. | 2004 | 15006820 |
| non-fermenters in human infections. | one thousand and one hundred thirty non-fermenting gram negative bacteria were isolated from various samples. of these, pseudomonas aeruginosa was the commonest isolate (72.83%) followed by acinetobacter anitratus (8.4%), alcaligenes faecalis (7.6%), acinetobacter lwoffi (4.4%), pseudomonas flourescens (2.4%), schwanella putrefaciens (1.6%), stenotrophomonas maltophilia (1.6%), pseudomonas putida (0.4%), bravundimonas vesicularis (0.4%) and flavobacterium meningosepticum (0.4%). antibiotic sensi ... | 2003 | 15022936 |
| identification of the bacterial community of maple sap by using amplified ribosomal dna (rdna) restriction analysis and rdna sequencing. | the bacterial community of maple sap was characterized by analysis of samples obtained at the taphole of maple trees for the 2001 and 2002 seasons. among the 190 bacterial isolates, 32 groups were formed according to the similarity of the banding patterns obtained by amplified ribosomal dna restriction analysis (ardra). a subset of representative isolates for each ardra group was identified by 16s rrna gene fragment sequencing. results showed a wide variety of organisms, with 22 different genera ... | 2004 | 15066796 |
| surface glycans of candida albicans and other pathogenic fungi: physiological roles, clinical uses, and experimental challenges. | although fungi have always been with us as commensals and pathogens, fungal infections have been increasing in frequency over the past few decades. there is a growing body of literature describing the involvement of carbohydrate groups in various aspects of fungal disease. carbohydrates comprising the cell wall or capsule, or as a component of glycoproteins, are the fungal cell surface entities most likely to be exposed to the surrounding environment. thus, the fungus-host interaction is likely ... | 2004 | 15084502 |
| mapping of the binding site on pseudoazurin in the transient 152 kda complex with nitrite reductase. | nitrite reductase (nir) catalyzes the reduction of nitrite to nitrite oxide as a part of the denitrification process. in alcaligenes faecalis s-6, the copper protein pseudoazurin acts as electron donor to nir. the binding surface of pseudoazurin involved in the formation of the 152 kda complex with nir has been determined by nmr using cross saturation from nir to perdeuterated pseudoazurin. due to the transient nature of the complex, saturation effects can be observed on the resonances of the un ... | 2004 | 15125645 |
| cloning, overexpression, and characterization of a novel thermostable penicillin g acylase from achromobacter xylosoxidans: probing the molecular basis for its high thermostability. | the gene encoding a novel penicillin g acylase (pga), designated pgaw, was cloned from achromobacter xylosoxidans and overexpressed in escherichia coli. the pgaw gene contains an open reading frame of 2586 nucleotides. the deduced protein sequence encoded by pgaw has about 50% amino acid identity to several well-characterized pgas, including those of providencia rettgeri, kluyvera cryocrescens, and escherichia coli. biochemical studies showed that the optimal temperature for this novel pga (pga6 ... | 2004 | 15128530 |
| arsenite oxidation by the heterotroph hydrogenophaga sp. str. nt-14: the arsenite oxidase and its physiological electron acceptor. | heterotrophic arsenite oxidation by hydrogenophaga sp. str. nt-14 is coupled to the reduction of oxygen and appears to yield energy for growth. purification and partial characterization of the arsenite oxidase revealed that it (1). contains two heterologous subunits, aroa (86 kda) and arob (16 kda), (2). has a native molecular mass of 306 kda suggesting an alpha(3)beta(3) configuration, and (3). contains molybdenum and iron as cofactors. although the hydrogenophaga sp. str. nt-14 arsenite oxidas ... | 2004 | 15178476 |
| multifocal detection of multidrug-resistant pseudomonas aeruginosa producing the per-1 extended-spectrum beta-lactamase in northern italy. | forty-four nonreplicate clinical isolates of pseudomonas aeruginosa that were resistant to extended-spectrum cephalosporins (ceftazidime and cefepime) and aztreonam, that putatively produced an acquired extended- spectrum beta-lactamase (esbl), according to the results of a double-disk synergy test, and that had been involved in nosocomial outbreaks were obtained from six different hospitals in northern italy and screened for the presence of bla(per) esbl determinants. twenty isolates, associate ... | 2004 | 15184430 |
| characterization of beta-glucan recognition site on c-type lectin, dectin 1. | dectin 1 is a mammalian cell surface receptor for (1-->3)-beta-d-glucans. since (1-->3)-beta-d-glucans are commonly present on fungal cell walls, it has been suggested that dectin 1 is important for recognizing fungal invasion. in this study we tried to deduce the amino acid residues in dectin 1 responsible for beta-glucan recognition. hek293 cells transfected with mouse dectin 1 cdna could bind to a gel-forming (1-->3)-beta-d-glucan, schizophyllan (spg). the binding of spg to a dectin 1 transfe ... | 2004 | 15213161 |
| effect of alcaligenes faecalis on nitrous oxide emission and nitrogen removal in three phase fluidized bed process. | nitrous oxide (n2o), one of the greenhouse effect gases, has not been known that how much n2o is produced from municipal wastewater treatment and what its management should be. in this study, for controlling nitrous oxide emission and removing nitrogen from municipal wastewater, we experimented the three phase fluidized bed process equipped with draft tube along with immobilized alcaligenes faecalis, a typical heterotrophic nitrifer and a predominant genus. also we evaluated the optimum treatmen ... | 2004 | 15242127 |
| 1h, 13c and 15n resonance assignment of cu(i)-pseudoazurin from alcaligenes faecalis s-6. | 2004 | 15243189 | |
| cpndb: a chaperonin sequence database. | type i chaperonins are molecular chaperones present in virtually all bacteria, some archaea and the plastids and mitochondria of eukaryotes. sequences of cpn60 genes, encoding 60-kda chaperonin protein subunits (cpn60, also known as groel or hsp60), are useful for phylogenetic studies and as targets for detection and identification of organisms. conveniently, a 549-567-bp segment of the cpn60 coding region can be amplified with universal pcr primers. here, we introduce cpndb, a curated collectio ... | 2004 | 15289485 |
| characterization of slac: a small laccase from streptomyces coelicolor with unprecedented activity. | laccases and other four-copper oxidases are usually constructed of three domains: domains one and three house the copper sites, and the second domain often helps form a substrate-binding cleft. in contrast to this arrangement, the genome of streptomyces coelicolor was found to encode a small, four-copper oxidase that lacks the second domain. this protein is representative of a new family of enzymes--the two-domain laccases. disruption of the corresponding gene abrogates laccase activity in the g ... | 2004 | 15295117 |
| bidirectional catalysis by copper-containing nitrite reductase. | the copper-containing nitrite reductase from alcaligenes faecalis s-6 was found to catalyze the oxidation of nitric oxide to nitrite, the reverse of its physiological reaction. thermodynamic and kinetic constants with the physiological electron donor pseudoazurin were determined for both directions of the catalyzed reaction in the ph range of 6-8. for this, nitric oxide was monitored by a clark-type electrode, and the redox state of pseudoazurin was measured by optical spectroscopy. the equilibr ... | 2004 | 15301545 |
| microdiversity of phenol hydroxylase genes among phenol-degrading isolates of alcaligenes sp. from an activated sludge system. | enterobacterial repetitive intergenic consensus (eric)-pcr fingerprinting classified 97 phenol-degrading isolates with identical amplified ribosomal dna restriction analysis (ardra) patterns into six genotypic groups. the 16s rrna gene of the representative isolate of each group had higher than 99.47% common identity with each other and higher than 98% identity with the type strain of alcaligenes faecalis. pcr-tgge (temperature gradient gel electrophoresis) analysis of the genes of the largest s ... | 2004 | 15321685 |
| biofuel cells select for microbial consortia that self-mediate electron transfer. | microbial fuel cells hold great promise as a sustainable biotechnological solution to future energy needs. current efforts to improve the efficiency of such fuel cells are limited by the lack of knowledge about the microbial ecology of these systems. the purposes of this study were (i) to elucidate whether a bacterial community, either suspended or attached to an electrode, can evolve in a microbial fuel cell to bring about higher power output, and (ii) to identify species responsible for the el ... | 2004 | 15345423 |
| molecular fingerprinting of dairy microbial ecosystems by use of temporal temperature and denaturing gradient gel electrophoresis. | numerous microorganisms, including bacteria, yeasts, and molds, constitute the complex ecosystem present in milk and fermented dairy products. our aim was to describe the bacterial ecosystem of various cheeses that differ by production technology and therefore by their bacterial content. for this purpose, we developed a rapid, semisystematic approach based on genetic profiling by temporal temperature gradient electrophoresis (ttge) for bacteria with low-g+c-content genomes and denaturing gradien ... | 2004 | 15345452 |
| evaluation of the new vitek 2 card for identification of clinically relevant gram-negative rods. | the vitek 2 card for gram-negative bacteria (biomérieux,marcy-l'etoile, france) has been redesigned to improve the identification of fermenting and nonfermenting bacilli. forty-seven biochemical tests, including 19 enzymatic tests, are present in the new card and interpreted in a kinetic mode. final identification results are available within 10 h. the database allows the identification of 159 different taxa. six hundred fifty-five gram-negative rods (gnr; 511 fermenters and 144 nonfermenters), ... | 2004 | 15364991 |
| bacteremia caused by achromobacter and alcaligenes species in 46 patients with cancer (1989-2003). | achromobacter and alcaligenes are emerging infectious gram-negative bacterial species that can affect immunosuppressed patients. the authors sought to determine the incidence and characteristics of bloodstream infections caused by these organisms in patients with underlying malignancies. | 2004 | 15389476 |
| beta-1,3 glucan sulfate, but not beta-1,3 glucan, induces the salicylic acid signaling pathway in tobacco and arabidopsis. | sulfate substituents naturally occurring in biomolecules, such as oligosaccharides and polysaccharides, can play a critical role in major physiological functions in plants and animals. we show that laminarin, a beta-1,3 glucan with elicitor activity in tobacco (nicotiana tabacum), becomes, after chemical sulfation, an inducer of the salicylic acid (sa) signaling pathway in tobacco and arabidopsis thaliana. in tobacco cell suspensions, the oxidative burst induced by the laminarin sulfate ps3 was ... | 2004 | 15494557 |
| identification and characterization of sulfolobus solfataricus d-gluconate dehydratase: a key enzyme in the non-phosphorylated entner-doudoroff pathway. | the extremely thermoacidophilic archaeon sulfolobus solfataricus utilizes d-glucose as a sole carbon and energy source through the non-phosphorylated entner-doudoroff pathway. it has been suggested that this micro-organism metabolizes d-gluconate, the oxidized form of d-glucose, to pyruvate and d-glyceraldehyde by using two unique enzymes, d-gluconate dehydratase and 2-keto-3-deoxy-d-gluconate aldolase. in the present study, we report the purification and characterization of d-gluconate dehydrat ... | 2005 | 15509194 |
| growth inhibition of foodborne pathogens and food spoilage organisms by select raw honeys. | twenty-seven honey samples from different floral sources and geographical locations were evaluated for their ability to inhibit the growth of seven food spoilage organisms (alcaligenes faecalis, aspergillus niger, bacillus stearothermophilus, geotrichum candidum, lactobacillus acidophilus, penicillium expansum, pseudomonas fluorescens) and five foodborne pathogens (bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, salmonella enterica ser. typhimurium, and staphylococcus aureus) ... | 2004 | 15527912 |
| high-rate nitrification at low ph in suspended- and attached-biomass reactors. | this article reports on high-rate nitrification at low ph in biofilm and suspended-biomass reactors by known chemolithotrophic bacteria. in the biofilm reactor, at low ph (4.3 +/- 0.1) and low bulk ammonium concentrations (9.3 +/- 3.3 mg.liter(-1)), a very high nitrification rate of 5.6 g of n oxidized.liter(-1).day(-1) was achieved. the specific nitrification rate (0.55 g of n.g of biomass(-1).day(-1)) was similar to values reported for nitrifying reactors at optimal ph. in the suspended-biomas ... | 2004 | 15528509 |
| bacterium-based no2- biosensor for environmental applications. | a sensitive no2- biosensor that is based on bacterial reduction of no2- to n2o and subsequent detection of the n2o by a built-in electrochemical n2o sensor was developed. four different denitrifying organisms lacking no3- reductase activity were assessed for use in the biosensor. the relevant physiological aspects examined included denitrifying characteristics, growth rate, no2- tolerance, and temperature and salinity effects on the growth rate. two organisms were successfully used in the biosen ... | 2004 | 15528518 |
| comparison of aerobic denitrifying activity among three cultural species with various carbon sources. | abilities of three aerobic denitrifiers such as alcaligenes faecalis, microvirgula aerodenitrificans and paracoccus pantotrophus were compared from the viewpoints of nitrate removal efficiency and organic matter utilization. first, the effect of carbon source was investigated. although nitrate reduction was observed in all strains under aerobic conditions, a change of carbon source considerably affected the denitrification ability. in the case of p. pantotrophus, nitrate and nitrite were complet ... | 2004 | 15566182 |