Publications

protein depletion using the arabinose promoter in xanthomonas citri subsp. citri.	xanthomonas citri subsp. citri (x. citri) is a plant pathogen and the etiological agent of citrus canker, a severe disease that affects all the commercially important citrus varieties, and has worldwide distribution. citrus canker cannot be healed, and the best method known to control the spread of x. citri in the orchards is the eradication of symptomatic and asymptomatic plants in the field. however, in the state of são paulo, brazil, the main orange producing area in the world, control is evo ...	2017	28343961
engineering canker-resistant plants through crispr/cas9-targeted editing of the susceptibility gene cslob1 promoter in citrus.	citrus canker, caused by xanthomonas citri subsp. citri (xcc), is severely damaging to the global citrus industry. targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. here, we report improvement of citrus canker resistance through crispr/cas9-targeted modification of the susceptibility gene cslob1 promoter in citrus. wanjincheng orange (citrus sinensis osbeck) harbors at least three copies of the cs ...	2017	28371200
bacterial and plant natriuretic peptides improve plant defense responses against pathogens.	plant natriuretic peptides (pnps) are implicated in the regulation of ions and water homeostasis, and their participation in the plant immune response has been also proposed. xanthomonas citri subsp. citri contains a gene encoding a pnp-like protein (xacpnp) that has no homologues in other bacteria. xacpnp mimics its arabidopsis thaliana homolog atpnp-a by modifying host responses to create favorable conditions for pathogen survival. however, the ability of xacpnp to induce plant defense respons ...	2017	28401640
the effect of citrus essential oils and their constituents on growth of xanthomonas citri subsp. citri.	citrus bacterial canker (cbc) caused by xanthomonas citri subsp. citri (xcc), is the most devastating of the citrus diseases worldwide. during our study, we found that essential oils (eos) of some citrus cultivars are effective on xcc. therefore, it prompted us to determine the plant metabolites responsible for the antibacterial properties. we obtained eos from some locally cultivated citrus by using a clevenger apparatus and their major constituents were identified by gas chromatography/mass sp ...	2017	28420101
'candidatus liberibacter asiaticus' encodes a functional salicylic acid (sa) hydroxylase that degrades sa to suppress plant defenses.	pathogens from the fastidious, phloem-restricted 'candidatus liberibacter' species cause the devastating huanglongbing (hlb) disease in citrus worldwide and cause diseases on many solanaceous crops and plants in the apiaceae family. however, little is known about the pathogenic mechanisms due to the difficulty in culturing the corresponding 'ca. liberibacter' species. here, we report that the citrus hlb pathogen 'ca. l. asiaticus' uses an active salicylate hydroxylase saha to degrade salicylic a ...	2017	28488467
tal effector driven induction of a sweet gene confers susceptibility to bacterial blight of cotton.	transcription activator-like (tal) effectors from xanthomonas citri subsp. malvacearum (xcm) are essential for bacterial blight of cotton (bbc). here, by combining transcriptome profiling with tal effector-binding element (ebe) prediction, we show that ghsweet10, encoding a functional sucrose transporter, is induced by avrb6, a tal effector determining xcm pathogenicity. activation of ghsweet10 by designer tal effectors (dtales) restores virulence of xcm avrb6 deletion strains, whereas silencing ...	2017	28537271
structural features of phox, one of the phosphate-binding proteins from pho regulon of xanthomonas citri.	in escherichia coli, the atp-binding cassette transporter for phosphate is encoded by the pstscab operon. psts is the periplasmic component responsible for affinity and specificity of the system and has also been related to a regulatory role and chemotaxis during depletion of phosphate. xanthomonas citri has two phosphate-binding proteins: psts and phox, which are differentially expressed under phosphate limitation. in this work, we focused on phox characterization and comparison with psts. the ...	2017	28542513
sulfate-binding protein (sbp) from xanthomonas citri: structure and functional insights.	the uptake and transport of sulfate in bacteria is mediated by an atp-binding cassette transporter (abc transporter) encoded by sbpcysuwa genes, whose importance has been widely demonstrated due to their relevance in cysteine synthesis and bacterial growth. in xanthomonas citri, the causative agent of canker disease, the expression of components from this abc transporter and others related to uptake of organic sulfur sources has been shown during in vitro growth cultures. in this work, based on ...	2017	28562158
proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of xanthomonas citri subsp. citri during citrus sinensis infection.	xanthomonas citri subsp. citri (xac) is the causal agent of citrus canker. a proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of xac during infection.	2017	28693412
complete genome sequences of three xanthomonas citri strains from texas.	the complete genome sequences of three xanthomonas citri strains isolated from lime trees in texas were found to belong to the a(w) group. all carried nearly identical large plasmids with similarity to those of a citrus canker strain from india and to xanthomonads from africa and colombia. all three strains harbored unusual ptha homologs.	2017	28705973
xanthomonas citri minc oscillates from pole to pole to ensure proper cell division and shape.	xanthomonas citri (xac) is the causal agent of citrus canker, a disease that affects citrus crops and causes economic impact worldwide. to further characterize cell division in this plant pathogen, we investigated the role of the protein minc in cell division, chromosome segregation, and peptidoglycan incorporation by deleting the gene minc using allele exchange. xac with minc deleted exhibited the classic δmin phenotype observed in other bacteria deleted for min components: minicells and short ...	2017	28769912
the genome of the cotton bacterial blight pathogen xanthomonas citri pv. malvacearum strain msct1.	xanthomonas citri pv. malvacearum is a major pathogen of cotton, gossypium hirsutum l.. in this study we report the complete genome of the x. citri pv. malvacearum strain msct1 assembled from long read dna sequencing technology. the msct1 genome is the first x. citri pv. malvacearum genome with complete coding regions for x. citri pv. malvacearum transcriptional activator-like effectors. in addition functional and structural annotations are presented in this study that will provide a foundation ...	2017	28770027
ectopic expression of xylella fastidiosa rpff conferring production of diffusible signal factor in transgenic tobacco and citrus alters pathogen behavior and reduces disease severity.	the pathogenicity of xylella fastidiosa is associated with its ability to colonize the xylem of host plants. expression of genes contributing to xylem colonization are suppressed, while those necessary for insect vector acquisition are increased, with increasing concentrations of diffusible signal factor (dsf) whose production is dependent on rpff. we previously demonstrated that transgenic citrus plants ectopically expressing rpff from a citrus strain of x. fastidiosa subsp. pauca exhibited les ...	2017	28777044
crystal structure and regulation of the citrus pol iii repressor maf1 by auxin and phosphorylation.	maf1 is the main rna polymerase (pol) iii repressor that controls cell growth in eukaryotes. the citrus ortholog, csmaf1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. we solved the crystal structure of the globular core of csmaf1, which reveals additional structural elements compared with the previously available structure of hmaf1, and explored the dynamics of its flexible regions not present in the structure. csmaf1 ...	2017	28781084
xanthomonas adaptation to common bean is associated with horizontal transfers of genes encoding tal effectors.	common bacterial blight is a devastating bacterial disease of common bean (phaseolus vulgaris) caused by xanthomonas citri pv. fuscans and xanthomonas phaseoli pv. phaseoli. these phylogenetically distant strains are able to cause similar symptoms on common bean, suggesting that they have acquired common genetic determinants of adaptation to common bean. transcription activator-like (tal) effectors are bacterial type iii effectors that are able to induce the expression of host genes to promote i ...	2017	28854875