Publications

TitleAbstractYear
Filter
PMID(sorted ascending)
Filter
fluctuations in s-adenosyl-l-methionine (adomet) during mitotic cycle of the myxomycete physarum polycephalum.a sensitive radioisotope dilution method was used to measure the s-adenosyl-l-methionine (adomet) content in macroplasmodia of the slime mold physarum polycephalum during the mitotic cycle. the adomet pool had two maxima, one during mitosis, the other in the middle of g2 phase.19853896825
[electron microscope study of changes in the chromatin structure in different functional states of nuclei of a ciliate bursaria truncatella and a myxomycete physarum polycephalum].chromatin structural organization was studied by means of electron microscopy in the macronuclei of ciliate bursaria truncatella at various stages of the life cycle (at different time intervals after cell division, in resting cysts and at excysting) and in the nuclei of myxomycete physarum polycephalum during the mitotic cycle. inactive chromatin was shown to be organized in compact clumps 100-300 nm in diameter linked with each other, their loop organization being convincingly demonstrated. upo ...19853920508
phylogenetic relationships among eukaryotic kingdoms inferred from ribosomal rna sequences.phylogenetic trees among eukaryotic kingdoms were inferred for large- and small-subunit rrnas by using a maximum-likelihood method developed by felsenstein. although felsenstein's method assumes equal evolutionary rates for transitions and transversions, this is apparently not the case for these data. therefore, only transversion-type substitutions were taken into account. the molecules used were large-subunit rrnas from xenopus laevis (animalia), rice (plantae), saccharomyces cerevisiae (fungi) ...19853932662
identification of histone h1(0) in physarum polycephalum. its high level in the plasmodial stage increases in amount and phosphorylation in the sclerotial stage.an antiserum specific for the globular domain of the bovine very lysine-rich histone subfraction h1(0) cross-reacted with a single protein band in the chromosomal proteins isolated from microplasmodia of the true slime mold physarum polycephalum. its amino acid composition was characteristic of a very lysine-rich histone which supported its identification as physarum h1(0). unlike physarum h1(0), which is 50% larger than mammalian h1, physarum h1(0) migrated very close to bovine h1(0) on sodium ...19863944138
role of mrna 5'-terminal caps in translational dormancy of physarum polycephalum.translational regulation of protein synthesis accompanies sclerotization in physarum polycephalum. plasmodial and sclerotial poly(a)+ rna were translated in a message-dependent wheat germ lysate in the presence of the cap analogue 7-methylguanosine-triphosphate to determine whether 5' structural alterations in mrna accompany translational repression. the translation of plasmodial and sclerotial poly(a)+ rna was reduced to identical levels suggesting that both rna populations are capped. the 5'-t ...19863947335
actin-fragmin interactions as revealed by chemical cross-linking.a one to one complex of actin and fragmin (a capping protein from physarum polycephalum plasmodia) was cross-linked with 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide. the cross-linking reaction generated two cross-linked products with slightly different molecular weights (88 000 and 90 000) as major species. they were cross-linked products of one actin and one fragmin. the cross-linking site of fragmin in the actin sequence was determined by peptide mappings [sutoh, k. (1982) biochemistry 21 ...19863955005
oscillations in cell shape and size during locomotion and in contractile activities of physarum polycephalum, dictyostelium discoideum, amoeba proteus and macrophages.changes in cell shape and size were measured during locomotion, together with the motive force of the protoplasmic streaming, in various amoeboid cells in different stages of their life cycle, and under various environmental conditions. the variations in these measurements with time were examined by fourier spectral analysis. notwithstanding a change in cell type in the life cycle of p. polycephalum, myxamoebae and tiny plasmodia showed a similar time pattern of locomotion, exhibiting oscillatio ...19853965294
the effect of heat shock on the cell cycle regulation of tubulin expression in physarum polycephalum.in the myxomycete physarum polycephalum, tubulin synthesis is subject to mitotic cycle control. virtually all tubulin synthesis is limited to a 2-h period immediately preceding mitosis, and the peak of tubulin protein synthesis is accompanied by a parallel increase in the level of tubulin mrna. the mechanism by which the accumulation of tubulin mrna is turned on and off is not clear. to probe the relationship between tubulin regulation and cell cycle controls, we have used heat shocks to delay m ...19853968184
alternative pathway of respiration in physarum polycephalum plasmodia.the external application of inhibitors of glycolysis in the presence of kcn shows a lethal effect on plasmodia of physarum polycephalum. however, alpha-ketoglutarate, but not succinate, maintains the contraction-relaxation cycle of plasmodial actomyosin in spite of the fact that glycolysis and cytochrome oxidase are inhibited. the oscillations supported by ketoglutarate disappear in the presence of sham, an inhibitor of alternative oxidase. these results imply the existence of kcn-resistant, alt ...19853978717
lack of nucleosomal structure in a dnase-i-solubilized transcriptionally active chromatin fraction of physarum polycephalum.light treatment of nuclei of physarum polycephalum microplasmodia with dnase i, at low mgcl2 concentration (less than or equal to 3% dna acid solubility, 0.1 mm mgcl2) selectively solubilizes a defined fraction of chromatin, in the form of a macromolecular complex. this fraction (up to 15% of the total chromatin) contains a full complement of the core histones and a reduced amount of histone h1, and is enriched in the high-mobility-group type of proteins. it is preferentially associated with nas ...19853979388
phosphorylation of histone h1 through the cell cycle of physarum polycephalum. 24 sites of phosphorylation at metaphase.h1 phosphorylation has been studied through the precise nuclear division cycle of physarum polycephalum. the number of sites of phosphorylation of physarum h1 is very much larger than the number of sites reported for mammalian h1 molecules which is consistent with the larger molecular weight of physarum h1. at metaphase all of the physarum h1 molecules contain 20-24 phosphates. immediately following metaphase, these metaphase-phosphorylated h1 molecules undergo rapid dephosphorylation to give an ...19853988745
recurrent senescence in axenic cultures of physarum polycephalum.when subcultures of the aux-2 and aux-4 strains of physarum polycephalum, which had been grown for more than four years in axenic shake culture, were transferred to non-axenic surface culture they displayed progressively shorter lifespans (older axenic surface cultures yield shorter lived non-axenic cultures). similar subcultures transferred to axenic agar medium also underwent senescent-like events. these subcultures, after a period of vigorous growth, displayed a slower growth rate, reduced cy ...19853989512
cell cycle-dependent variations in rna polymerase activity in the nuclear and cytoplasmic fractions of physarum polycephalum.the rna polymerase activities of the soluble enzymes from the nuclear and cytoplasmic fractions from synchronous macroplasmodia of physarum polycephalum were measured. activity in the nuclear fraction prepared from cells in the g2 phase was several times higher than that at metaphase, and activity in the cytoplasmic fraction was much higher at metaphase than in the g2 phase. the amount of activity was nearly constant during the late g2 phase and early prophase, then rose abruptly just before met ...19853995603
multiple forms of actin in physarum polycephalum.actin in the acellular slime mold physarum polycephalum consists of three major forms closely spaced at isoelectric point (ip) 4.7 and a minor form at ip 5.1. amino acid analysis has shown the ip 5.1 actin to be nearly identical to the 4.7 actins. in actin purified from acetone powder, both actin forms were present. both forms bound to dnase i and have the same molecular weight of about 43 000 on sodium dodecyl sulfate (sds) polyacrylamide gels. on 2-d gels of nuclear proteins, both forms of act ...19853996429
complementation of npf mutations in diploid amoebae of physarum polycephalum: the basis for a general method of complementation analysis at the amoebal stage. 19853996912
use of monoclonal antibodies to analyse the expression of a multi-tubulin family.we have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in physarum polycephalum. three anti-beta-tubulin monoclonal antibodies, dm1b, dm3b3 and kmx-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. however, these antibodies showed a spectrum of reduced reactivity with the plasmodial beta 2-tubulin isotype - the competence of recognition of this isotype was graded dm1b greater than kmx-1 greater than dm3b3. the anti ...19854018260
a plasmodial alpha-tubulin cdna from physarum polycephalum. nucleotide sequence and comparative analysis.as the first step towards correlating structure and function of tubulin in the slime mold physarum polycephalum we have elucidated the nucleotide sequence of a cdna that appears to code for all but the last 25 to 30 c-terminal amino acids of a plasmodial alpha-tubulin. differences in amino acid sequence from those of other alpha-tubulins are distributed fairly evenly throughout the sequence, although a relatively extensive conserved region is found in position 396 to 426 near the c terminus. a s ...19854020874
physical studies by nmr and circular dichroism determining three structurally different domains in physarum polycephalum histone h1.combined studies which include, nmr spectroscopy, circular dichroism, amino acid analysis and polyacrylamide gel electrophoresis together show that the protein designated as histone h1 from physarum polycephalum has many of the features of histone h1 derived from other sources. the molecular masses of the globular peptide and the whole molecule were found to be 9000 +/- 1000 da and 33000 +/- 3000 da respectively. nmr melting experiments showed that the half-melt temperature was 53 +/- 1 degree c ...19854029150
[structural characteristics and availability of dna for solvent molecules in nuclei of physarum polycephalum].the rate constants of 1h--3h exchange between water and c8h-groups of purinic residues of dna in solution and in nuclei of synchronous physarum polycephalum at different phases of the cell cycle were measured. the nuclear membranes and nonhistone proteins were shown not to create additional hindrances and not to diminish the availability of dna in nuclei for solvent molecules. a small increase of the rate of the 1h--3h exchange between water and the dna of nuclei upon transition from the s-phase ...19854033643
immunological analysis of histone h2a from the slime mold physarum polycephalum.specific antibodies against the histone h2a from calf thymus were generated by injecting rabbits with complexes: histone h2a-rna with a protein to rna ratio of 3:1. in the microcomplement fixation assay the antibodies against the histone h2a from calf thymus immuno-reacted with the histone h2a from calf thymus but not with h2a from physarum polycephalum. the histone h2a from calf thymus therefore appears to have an immunological determinant(s) which does not exist in h2a from physarum polycephal ...19854036445
a method for isolation of cytoplasmic rna from a slime mold, physarum polycephalum.a procedure for fast and simple preparation of cytoplasmic ribonucleic acid from physarum polycephalum microplasmodia is described. microplasmodia are homogenized in a high-magnesium-high-ethylene glycol bis(beta-aminoethyl ether) n,n'-tetraacetic acid buffer and nuclei are pelleted. the supernatant is extracted with sodium dodecyl sulfate-phenol-chloroform and crude rna is precipitated. this is further purified by selective ethanol precipitation from 6 m guanidinum hydrochloride. this rna prepa ...19854037303
a further assessment of the sub-nucleosomal product, "peak a", from physarum polycephalum.nuclease digestion studies of physarum polycephalum nuclei (1-3) and nucleoli (4) over the past few years have been centred on a number of modified nucleosomal products which have been related to active-gene regions of the genome. we have re-investigated one such particle, peak a, using the techniques of differential melting and polyacrylamide gel electrophoresis and show that this material is unlikely to be a specific histone:dna complex as suggested by earlier authors.19854038297
improved negative staining of microfilament arrangements in detergent-extracted physarum amoeboflagellates.a motile, lamellipodium-like structure, the ridge, forms as amoeboflagellate cells of physarum polycephalum release from a substratum and begin swimming in fluid. actin microfilaments form a distinct laminar core within the ridge; they are seen as a sparse, disordered meshwork in cytoskeletons prepared by conventional methods using uranyl acetate negative staining [10]. preservation and visualization of these filaments and their arrangements improved considerably when cytoskeletons were imaged w ...19854038387
enrichment of fibrillar cytoplasmic actomyosin in protoplasmic strands of physarum polycephalum for the production of cell-free models.the treatment of isolated protoplasmic strands of physarum polycephalum with 2.5% ethanol in a physiological salt solution under isometric conditions induces the formation of a large amount of mostly longitudinally organized actomyosin fibrils in the endoplasmic channel, a region normally free of actomyosin fibrils. the quantity of fibrillogenesis as well as the concomitant force output during the induced contractures are dependent on the ca++-content and the temperature of the test solution. th ...19854038920
changes in phosphorylation of an f-actin capping protein of physarum polycephalum during the cell cycle.the cap 42(b), a ca2+-dependent f-actin capping phosphoprotein of 42,000 daltons, was shown to be localized in the cytosol of physarum polycephalum by measurements of phosphorylatability in the absence of ca2+. the phosphorylation of cap 42(b) in the cytosol changed during the cell cycle: it was high in the s and g2 phase, and low in the m phase and boundary phase between s and g2 phase. when the isolated cap 42(b) was added to m phase cytosol, the phosphorylation of cap 42(b) was significantly ...19854044559
effects of the free radical generator paraquat on differentiation, superoxide dismutase, glutathione and inorganic peroxides in microplasmodia of physarum polycephalum.the herbicide paraquat was used to investigate the effects of oxidative stress on the spherulation of physarum polycephalum microplasmodia. the responses of a white non-differentiating strain of physarum were compared with those of a common yellow strain that readily spherulates in salts-only starvation medium. the addition of paraquat to the salts medium increased the specific activity of superoxide dismutase in both strains; it also induced an increase in the intracellular inorganic peroxide c ...19854064105
phosphorylation of histidine in proteins by a nuclear extract of physarum polycephalum plasmodia.a high salt nuclear extract from the true slime mold physarum polycephalum was used as a source of kinase activity for the incubation of calf thymus histones with [gamma-32p]atp. a major proportion of the 32p incorporated into histones was acid-labile and alkali-stable. the nature of the alkali-stable phosphorylated component was analyzed by subjecting the phosphorylated protein to total alkaline hydrolysis and separating the resultant phosphoamino acids by anion exchange chromatography. the 32p ...19854066704
alterations in superoxide dismutase, glutathione, and peroxides in the plasmodial slime mold physarum polycephalum during differentiation.changes in the level of antioxidant defenses and the concentration of free radical by-products were examined in differentiating (m3cvii and lu897 x lu863), non-differentiating (lu887 x lu897), and heterokaryon microplasmodia of the slime mold physarum polycephalum during spherulation in salts-only medium. as differentiation proceeded, superoxide dismutase activity increased by as much as 46 fold; glutathione concentration and the rate of oxygen consumption decreased; cyanide-resistant respiratio ...19854066766
in vivo transcription of ribosomal rna in relation to the mitotic cycle in physarum polycephalum.we have investigated the transcription of ribosomal rna in plasmodia of physarum polycephalum by a combination of pulse-labelling with [3h]uridine and rna:dna hybridization. the dna used for the hybridization was a hindiii restriction fragment (cloned in bacteriophage lambda) of physarum ribosomal dna that carries a substantial fraction of the rrna genes, enabling the ribosomal transcripts in the newly synthesized rna to be measured. we found that ribosomal rna constituted about 40% of the pulse ...19854066783
growth and differentiation of wild type amoebae of physarum polycephalum in liquid culture.a method for growing wild type amoebal strains of physarum polycephalum in two-membered liquid culture is presented. the medium is a simple buffered salts solution. we found that a minimal level of divalent cation was required for growth. all amoebal strains tried to date have grown under our conditions in stationary culture. growth under gyratory conditions was only successful at 60 rpm or less and consistent growth required a period of adaptation over several transfers. differentiation of two ...19854075407
studies on mitochondrial structure and function in physarium polycephalum. i. fine structure, cytochemistry, and 3 h-uridine autoradiography of a central body in mitochondria. 19734121445
changes in ribonucleases during differentiation (spherulation) of physarum polycephalum. 19734123060
effects of supraoptimal temperatures on the myxomycete physarum polycephalum. ii. effects on the rate of protein and ribonucleic acid synthesis. 19744134518
studies on the regulation of carbohydrate synthesis during growth and differentiation of physarum polycephalum. i. activity of udpgpyrophosphorylase during spherulation. 19744136207
studies on mitochondrial structure and function in physarum polycephalum. iii. electron microscopy of a large amount of dna releases from a central body in mitochondria by trypsin digestion. 19744138519
plasmodial mitoses and polyploidy in the myxomycete physarum polycephalum. 19664161839
periodic thymidine kinase production in synchronous plasmodia of physarum polycephalum: inhibition by actinomycin and actidion. 19674168409
natural synchrony in the slime mould physarum polycephalum. 19684176076
division stages of the mitochondria in normal and actinomycin-treated plasmodia of physarum polycephalum. 19694179727
[research on stabilization of intracytoplasmic membranous formations of chondromyces crocatus by conjugate action of adenosine triphosphate and ruthenium tetroxide]. 19704193632
electron microscope observations on actomyosin and actin preparations from physarum polycephalum, and on their interaction with heavy meromyosin subfragment i from muscle myosin. 19704196079
activities of dna degrading enzymes in the slime mold physarum polycephalum. evidence for five different enzymes. 19744209281
dna replication continued in isolated nuclei of synchronously growing physarum polycephalum. 19744218840
limited dna synthesis in the absence of protein synthesis in physarum polycephalum.actidione (cycloheximide), an antibiotic inhibitor of protein synthesis, blocked the incorporation of leucine and lysine during the s phase of physarum polycephalum. actidione added during the early prophase period in which mitosis is blocked totally inhibited the initiation of dna synthesis. actidione treatment in late prophase, which permitted mitosis in the absence of protein synthesis, permitted initiation of a round of dna replication making up between 20 and 30% of the unreplicated nuclear ...19664226393
functionality of newly synthesized dna as related to rna synthesis during mitotic cycle in physarum polycephalum. 19694241920
some biochemical events in the growth cycles of physarum polycephalum. 19694242861
polyphosphate in the life cycle of physarum polycephalum and its relation to rna synthesis. 19694243909
initiation of synchronous mitosis in physarum polycephalum. a model of the control of cell division in eukariots. 19724260954
macromolecular syntheses and mitosis in uv-irradiated plasmodia of physarum polycephalum. 19724262002
the biosynthesis of plasmodial myosin during starvation of physarum polycephalum.the actomyosin protein complex of physarum polycephalum was prepared from vegetative and starved plasmodia. the yield of actomyosin per unit wet wt. was the same from both types of plasmodia. myosin was resolved from the complex by gel filtration and purified by ion-exchange chromatography. the ca(2+)-stimulated adenosine triphosphatase activities of myosin preparations from vegetative and starved plasmodia were not appreciably different. synthesis of myosin de novo was shown to occur during the ...19734273185
adenosine 3',5'-monophosphate phosphodiesterase in the growth medium of physarum polycephalum.the acellular slime mold physarum polycephalum releases a soluble adenosine 3', 5'-monophosphate phosphodiesterase into the growth medium. although this enzyme resembles the particulate diesterase of the same organism in kinetic properties and in inhibition by methyl purines, its greater stability, its insensitivity to stimulation by imidazole and to inhibition by adenosine triphosphate,- and its selective release into the medium indicate a specific function, perhaps protection against exogenous ...19714321650
activity of some enzymes in physarum polycephalum. 3. during spherulation (differentiation) induced by mannitol. 19714327869
an adenosine 3',5'-monophosphate-inhibited protein kinase from physarum polycephalum. 19714331389
poly(adenosine diphosphate ribose) polymerase in physarum polycephalum nuclei. 19714336158
[differential protein synthesis during differentiation of physarum polycephalum: demonstration through density labeling]. 19724341661
cell cycle variation in cyclic adenosine 3',5'-monophosphate-dependent inhibition of protein kinase from physarum polycephalum. 19724344888
plasmodial ultrastructure of the myxomycete physarum polycephalum. 19724345935
phospholipids and phospholipase d in the true slime mold physarum polycephalum. 19734352920
studies on the biosynthesis of cytochrome c.a soluble cytochrome was isolated and purified from the slime mould physarum polycephalum and identified as cytochrome c by room-temperature and low-temperature (77 degrees k) difference spectroscopy. a close similarity between p. polycephalum and mammalian cytochromes c was suggested by a comparison of the initial rates of oxidation of both proteins by mammalian mitochondria. this similarity was further emphasized by redox titrations and gel-electrophoretic studies which indicated that p. polyc ...19734353090
purification and properties of cytoplasmic and mitochondrial malate dehydrogenases of physarum polycephalum.two isoenzymes of malate dehydrogenase (mdh) were demonstrated in plasmodia of physarum polycephalum by polyacrylamide-gel electrophoresis. the more "cathodal" form was uniquely associated with mitochondria (m-mdh) and the other form was found in the soluble cytoplasm (s-mdh). the isoenzymes were separated by acetone fractionation of soluble plasmodial homogenates acidified to ph 5.0. the m-mdh was purified 201-fold by cetylpyridinium chloride treatment, fractionation with ammonium sulfate, grad ...19734355490
the electron-transport system of mitochondria from the slime mould physarum polycephalum.a method is described for the preparation of mitochondria from the slime mould physarum polycephalum; the mitochondria were not coupled. p. polycephalum mitochondria oxidized added nadh via a rotenone-insensitive pathway, but the oxidation of malate plus glutamate was rotenone sensitive; both of these substrates reduced much less cytochrome b than did succinate, in both aerobic and anaerobic steady states. spectroscopy at 77 degrees k separated three absorption maxima in the alpha-band region, a ...19734356125
control of dna replication in physarum polycephalum. i. specific activity of nad pyrophosphorylase in isolated nuclei during the cell cycle. 19714360907
changes in the activity of selected enzymes during starvation of physarum polycephalum. 19744362961
advanced initiation of synchronous mitoses in physarum polycephalum following uv-irradiation. 19704394219
physical separation of nuclei from two independent plasmodia of physarum polycephalum after fusion. 19744407050
continuous nucleolar dna synthesis in late-interphase nuclei of physarum polycephalum after transplantation into post-mitotic plasmodia. 19744407322
analysis of isoaccepting trnas during the growth phase mitotic cycle of physarum polycephalum. 19744471998
ultrastructural effects of griseofulvin on the myxomycete physarum polycephalum. inhibition of mitosis and the production of microtubule crystals. 19744472522
change in properties of physarum polycephalum amoebae during extended culture.during two years of passage, several myxamoebal strains of physarum polycephalum changed with regard to their properties in crosses and plaque morphology. these changes have been correlated with increased nuclear deoxyribonucleic acid content.19744472784
the rapid intranuclear accumulation of preexisting proteins in response to high plasmodial density in physarum polycephalum. 19744473038
plasmodium formation without change in nuclear dna content in physarum polycephalum. 19744474107
uridine diphosphoglucose pyrophosphorylase activity and differentiation in the cellular slime mold physarum polycephaluno.the specific activity of uridine 5'-triphosphate:alpha-d-glucose 1-phosphate uridyltransferase (ec 2.7.7.9) (also called uridine 5'-diphosphate [udp]-glucose pyrophosphorylase) has been found to increase up to eightfold during spherule formation by the slime mold physarum polycephalum. the enzyme accumulates during the first 8 to 9 h after initiation of spherule formation, declines to basal levels found in vegetative microplasmodia by 15 h, and is undetectable in completed spherules. specific ac ...19744474161
a method of indirect mutant selection in physarum polycephalum using the antibiotic netropsin. 19744474585
cycloheximide induced in vivo modification of ornithine decarboxylase in physarum polycephalum. 19744474953
letter: sites of dna synthesis in the mitochondria of physarum polycephalum as revealed by electron microscopic autoradiography. 19744475081
the preparation and preliminary characterisation of chromatin from the slime mould physarum polycephalum. 19744475636
methods for the isolation and analysis of plasmodial mutants in physarum polycephalum. 19744476673
studies on the mechanism of dna replication in physarum polycephalum. 19744476802
genetic analysis in the colonia strain of physarum polycephalum: heterothallic strains that mate with and are partially isogenic to the colonia strain.amoebae of the colonia (cl) strain, which normally produce plasmodia in clones, are shown to cross with mt3 and mt4 amoebae under appropriate conditions. strains carrying mt3 and mt4 in a cl genetic background were constructed and used in the genetic analysis of mutants isolated in cl.19744477090
the amount of dna coding for rrna during differentiation (spherulation) in physarum polycephalum. 19744477484
[the effect of heating the plasmodium physarum polycephalum on incorporation of the precursors of protein and rna synthesis in vivo]. 19744478032
correlation between unrepaired radiation-induced dna strand breaks and mitotic delay in physarum polycephalum. 19724507515
nuclear dna content and senescence in physarum polycephalum. 19734518990
purification and properties of two rna polymerases from physarum polycephalum.two rna polymerases have been purified from the slime mold physarum polycephalum, one sensitive and one resistant to alpha-amanitin. both enzymes are more active with denatured dna than native dna as a template and prefer mn(++) rather than mg(++) as a divalent cation. the alpha-amanitin-sensitive enzyme shows maximum activity at 0.15 m kcl, whereas the resistant enzyme is most active at very low ionic strength. analysis of the resistant enzyme on polyacrylamide gels containing sodium dodecyl su ...19744524629
cytofluorometric determination of dna and protein in the nuclei and nucleoli of physarum polycephalum during the intermitotic period. 19724562512
genetics and biochemistry of cycloheximide resistance in physarum polycephalum. 19724562869
phosphorylation of very-lysine-rich histone in physarum polycephalum. correlation with chromosome condensation. 19734570762
annular structures in isolated nuclei of physarum polycephalum. 19734587742
axenic culture of myxamoebae of the myxomycete physarum polycephalum.myxamoebae of the acellular slime mold physarum polycephalum have been cultured axenically in a soluble medium. the growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. cell densities ranging from 3 x 10(6) to 5 x 10(6) cells/ml have been obtained with this medium. to date, myxamoebae have been serially transferred more than 100 times without deleterious effect.19724591478
reassociation kinetics of nuclear dna from physarum polycephalum. 19744599836
scanning electron microscopy of freeze-fractured sclerotia of physarum polycephalum. 19744607050
plasmalemma invaginations as characteristic constituents of plasmodia of physarum polycephalum. 19744608528
somatic incompatibility following plasmodial fusion between strains of the myxomycete physarum polycephalum: the effect on their nuclei. 19744615132
[significance of thymidine phosphorylation for the s phase in the cell cycle of physarum polycephalum]. 19724672136
[characterization of a soluble dna polymerase from isolated nuclei of the synchronously growing myxomycete physarum polycephalum]. 19724672148
polysome profiles, amino acid incorporation in vitro, and polysome reaggregation following disaggregation by heat shock through the mitotic cycle in physarum polycephalum. 19724672224
dna replication in physarum polycephalum. 19724672237
acceleration of nuclear growth and advanced initiation of mitosis in u.v.-irradiated plasmodia of physarum polycephalum. 19724672343
differential protein synthesis during differentiation (spherulation) of physarum polycephalum: lack of synthesis of glucose-6-phosphate dehydrogenase. 19724672424
isolation and purification of the extracellular ribonuclease from physarum polycephalum. 19724672923
Displaying items 501 - 600 of 1460