Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| immunochemical properties of mannan-protein complex isolated from viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain by the action of zymolyase. | viable cells of saccharomyces cerevisiae 4484-24d-1 mutant strain were treated with an arthrobacter sp. beta-1,3-glucanase, zymolyase-60,000, in the presence of a serine protease inhibitor, phenylmethylsulfonyl fluoride. fractionation of the solubilized materials with cetavlon (cetyltrimethylammonium bromide) yielded a purified mannan-protein complex, which had a molecular weight of ca. 150,000, approximately three times higher than that of the mannan isolated from the same cells by the hot-wate ... | 1984 | 6398395 |
| lysis of nitrofurantoin-resistant strain of vibrio el tor. | both nitrofurantoin-sensitive and nitrofurantoin-resistant strains of vibrio el tor were found to lyze in the presence of tris-edta at alkaline ph. the rate of lysis was appreciably enhanced by lysozyme. the amounts of intracellular components, viz. proteins and carbohydrates, released from the nitrofurantoin-sensitive strain by tris-edta treatment, were significantly lower than those from the nitrofurantoin-resistant strain. differences in periplasmic proteins released from tris-edta treated ce ... | 1983 | 6403416 |
| dna interference with radioimmunoassay procedures. | a bacterial factor preventing precipitation of immune complexes was purified from a culture of streptococcus faecalis (ak strain) by gel filtration on sephadex g-100 and bio-gel a-150m columns. the factor was identified as dna on the basis of its ultraviolet absorption maximum at 260 nm, hydrolysis by deoxyribonuclease, mr exceeding 1.5 x 10(8), and positive reaction with diphenylamine. dna interferes with the immunoassay by preventing precipitation of immune complexes. only double-stranded dna ... | 1983 | 6414736 |
| [regulation of the enzyme activity of para-xylene catabolism in a spontaneous para-xylene-negative variant of pseudomonas aeruginosa 2x79]. | a spontaneous variant of pseudomonas aeruginosa 2x unable to grow on p-xylene as the sole source of carbon and energy has been isolated. p-xylenenegative variant of p. aeruginosa 2x79 differs from the wild type strain by the character of growth on the p-xylene oxidation intermediates p-toluate and protocatochuate. the cell of 2x79 variant inability to grow on p-xylene has been shown to be accompanied by the elimination of the activities of three enzymes - p-xylene methylhydroxylase, p-cresol met ... | 1983 | 6418220 |
| malate dehydrogenase isozymes in schistosoma japonicum (chinese mainland strain) by electrophoresis. | 1983 | 6418450 | |
| mechanical modeling of the stress adaptation process in bone. | the philosophy, techniques, and concepts involved in the mechanical modeling of the process of stress adaptation in bone are described here. first, the idea of a control surface, across which all mechanical forces and fluid transport are monitored, is introduced and employed to totally enclose a living whole bone. then the mechanical forces are related to local tissue stresses and the fluid transport to the local microcirculation. the concepts of strain, stress, and elasticity are introduced nex ... | 1984 | 6430529 |
| restoration of ligase activity in e. coli k12 lig ts7 strain by bacteriophage mu and cloning of a dna fragment harbouring the mu 'lig' gene. | restoration of ligase activity has been observed in e. coli k12 ligts7 strain lysogenic for mu, in presence as well in absence of lysogenic immunity. this restoration consist in phenotypic reversal of temperature sensitivity of e. coli ligts7 which also regain the ability to sustain the complete growth cycle of t4 lig-phages. it is possible to put under the control of the gal operon the expression of the viral gene responsible for the restoration effect. this new gene of mu has been named 'lig'. ... | 1980 | 6449688 |
| passive enhancement of rat heart allografts by xenogeneic antidonor sera raised in rabbits. | antilymphocyte serum (als) was raised in rabbits immunized by administration of lymphoid cells from sprague-dawley (spd) rats. the als was rendered specific for the spd strain by complete absorption by wistar rat lymphoid cells. the specificity was considered to be predominantly directed to spd ir gene-associated (ia) antigens, since absorption by spd erythrocytes failed to abolish lymphocytotoxic activities. alloantibody production by wistar rats sensitized with spd spleen cells was completely ... | 1982 | 6460361 |
| effects of triiodothyronine treatments on body and organ growth and the development of immune function in dwarf chickens. | the effects of triiodothyronine (t3) treatments on general body growth, long bone growth, primary lymphoid organ development, antibody production, and serum growth hormone (gh) and thyroid hormone levels were examined in two dwarf strains (sex-linked dwarf--sld, and autosomal dwarf--adw) and in a normal-growing control strain (k) of white leghorn chickens. one-day-old male chicks from each of these strains were assigned to either an untreated control group or to one of the groups receiving a t3 ... | 1984 | 6473358 |
| influence of phase feeding available phosphorus on egg production characteristics, carcass phosphorus content, and serum inorganic phosphorus levels of three commercial layer strains. | an experiment was conducted to obtain information on the concept of progressively decreasing dietary phosphorus levels (phase feeding) on the performance of three different commercial layer strains. dietary available phosphorus (ap) levels of .15, .30, or .45% were fed continuously from 22 to 70 weeks of age. concurrently, a diet containing .35, .25, and .15% ap was phase-fed during age intervals of 22 to 34, 34 to 50, and 50 to 70 weeks, respectively. dietary ap levels did not significantly aff ... | 1984 | 6483720 |
| biological properties and dog response to a variant (m-) strain of brucella canis. | wild type (virulent) b. canis organisms are always rough and growth becomes extremely mucoid (m+) after several days of incubation, especially on media ph 7.2. the mucoid growth of virulent (m+) b. canis and the marked tendency for m+ bacteria, and extracted cell wall antigens, to aggregate at acid ph (ph less than 6.5) are markedly diminished in a less mucoid (m-) variant. the m+ and m- brucella, and their extracted cell wall antigens, also differ in hydrophobic properties; the m+ organisms (an ... | 1984 | 6489639 |
| pneumonia due to chlamydia trachomatis in japanese infants. | sera from 109 japanese infants with pneumonia were tested for antibody to chlamydia trachomatis (c. trachomatis) l2 strain by an indirect immunofluorescence (if) technique. nasopharyngeal swabs were also collected to isolate c. trachomatis. clinical specimens were inoculated onto cycloheximide-treated mccoy cells and deae-dextran-treated hela 229 cells. of 109 patients, 32 (29%) were positive for igm antibodies (titer, greater than or equal to 1:16) to c. trachomatis. c. trachomatis was isolated ... | 1984 | 6495321 |
| isolation and identification of two hemolytic forms of streptolysin-o. | streptolysin-o was isolated from culture supernatants of group-a beta-hemolytic streptococci (richards strain) by ammonium sulfate and polyethylene glycol precipitation, deae-ion exchange chromatography, preparative isoelectric focusing, and chromatography on sephacryl s-300. two forms of the toxin possessing similar hemolytic capacity were identified. the native toxin was a single polypeptide chain devoid of amino sugars with a sedimentation coefficient of 3.9s and a molecular weight of 69,000, ... | 1984 | 6500696 |
| cross adaption between stress and cold in rats. | three-hour immobilization stress was imposed on male adult rats of wistar strain by restraining them on a board 6 days a week for 1-8 weeks. the stressed rats showed less body weight gain during the experiment compared to the controls. these stressed animals manifested an improved cold tolerance as shown by no significant fall in colonic temperature in the cold at -5 degrees c for 300 min during the experimental period, while the colonic temperature of the controls fell progressively. nonshiveri ... | 1984 | 6522247 |
| control of early embryonic heart morphogenesis: a hypothesis. | the early events of looping (rotation and bending) of the embryonic vertebrate heart are deformations. the deformative forces and their regulation are intrinsic to the heart. a model of cardiac morphogenesis is described in this paper. it is proposed that synthesis of extracellular matrix by the myocardium generates an internal pressure. this pressure acts as a deforming force on the myocardium which controls strain by regulating compliance. this model provides an explanation of cardiac morphoge ... | 1983 | 6557899 |
| utilization of plasmid pulb113 (rp4::mini-mu) to construct a linkage map of erwinia carotovora subsp. chrysanthemi. | we report experimental evidence that pulb113, an rp4::mini-mu plasmid, mediates chromosome transfer in a strain of erwinia carotovora subsp. chrysanthemi which does not accept the f episome. this allowed us to construct a genetic map of that strain by measuring the frequencies of cotransfer of different markers (thy, leu, pro, [his, trp], thya, rpsl, ile). | 1983 | 6574127 |
| nosocomial spread of haemophilus influenzae type b infection documented by outer membrane protein subtype analysis. | this report provides evidence of nosocomial transmission of haemophilus influenzae type b infection between two children in an acute care hospital. in the past, transmission of infection between these children would have gone unrecognized, because the isolates from the respective cases differed in susceptibility to ampicillin. however, the outer membrane protein profile of the two isolates was identical and has previously been observed in less than 2% of type b haemophilus isolates from patients ... | 1983 | 6602214 |
| [antigenic drift of a strain of influenza equi virus isolated in france during the winter of 1978-1979]. | a strain of influenza equi virus isolated during winter 1978-1979 has been compared with influenza a/equine/miami/1/63 (h3n8) strain by cross reactions performed by radial haemolysis (rh) and hemagglutination inhibition (hai) test. specific antisera were prepared on hens and guinea-pigs. results differed according to the species on which the sera were prepared and the two methods of titration of the antibodies. hens sera were unable to differenciate by hai the newly-isolated strain influenza a/e ... | 1983 | 6614786 |
| h-2 gene control of resistance to p815-x2 mastocytoma. | some, but not all, mutations at h-2 loci can alter, in either direction, histocompatible tumor resistance in the model system of p815-x2 mastocytoma (dba/2 origin) transplanted into dba/2j or (c57bl/6 x dba/2j)f1 mice. this hybrid effect has been shown previously to depend on the immune system. we have examined eight mutants at h-2kb (bm1, bm4, bm6, bm7, bm8, bm10, bm11, and bm16, two at h-2db (bm13 and bm14), and one i-ab mutant (bm12). when one parent is an h-2b mutant (c57bl/6 mutant), the hy ... | 1983 | 6640527 |
| attachment and internalization of a chlamydia trachomatis lymphogranuloma venereum strain by mccoy cells: kinetics of infectivity and effect of lectins and carbohydrates. | the kinetics of attachment and ingestion of chlamydia trachomatis serotype l1 by monolayers of mccoy cells were studied by using a method that discriminated between attachment and uptake. when about 1% of the mccoy cells was infected, the proteinase k-resistant chlamydial fraction, regarded as ingested chlamydiae, reached a constant value after about 3 h of incubation at 37 degrees c. uptake of chlamydiae at 4 degrees c could not be demonstrated. the attached and ingested chlamydial fractions we ... | 1983 | 6642670 |
| [reduction of local muscle strain by designing a pneumatic drill for assembly work]. | 1983 | 6649951 | |
| transepithelial chemotaxis of rat peritoneal exudate cells. | the migration of peritoneal exudate (pe) cells into plain millipore filters mounted in boyden chambers occurs under random, chemokinetic and chemotactic conditions. significant migration of such cells in vivo, however, involves both transendothelial and transepithelial penetration and occurs predominantly under pathological conditions where chemotactic agents are presumed to be present in gradient form. when madin-darby canine kidney (mdck) epithelial cells are grown as a confluent monolayer on ... | 1983 | 6661396 |
| [several regularities of the interrelation of blood leukocytes and microbes in the infectious process]. | the rate of absorption by polymorphonuclear leukocytes of different strains of staphylococci present in a homogeneous mixture was studied. the cells of one of the strains were radiolabeled and this made it possible to differentiate them from the cells of another strain by means of electron microscopic autoradiography. different rates of absorption of cocci belonging to different strains by leukocytes of normal subjects and by those from patients with sepsis resulting from burn or wound were obse ... | 1983 | 6670937 |
| legionella bozemanii serogroup 2: a new etiological agent. | a newly discovered bacterium, toronto 3, isolated from a lung aspirate of a patient with pneumonia, has been characterized. the isolate was identified as legionella bozemanii by chemical data from cellular fatty acid and ubiquinone analyses and by dna relatedness studies. the isolate, however, differs phenotypically from l. bozemanii by its colonial characteristics and strong interspecies serological cross-reactions, which are unique among clinical isolates of legionellae. the name l. bozemanni ... | 1984 | 6690465 |
| the effect of spermine on spontaneous and uv-induced mutations in schizosaccharomyces pombe. | the effect of different concentrations of spermine on spontaneous and uv-induced mutation in the adenine forward mutation system of schizosaccharomyces pombe was investigated. the effect of spermine on spontaneous mutation was studied in 5 mutator strains (mut 1-4, mut 1-23, mut 2-9, mut 2-20 and mut 3-21) and on uv-induced mutation in a pigmented adenine-requiring strain and its radiation-sensitive derivative (rad 13). the effect of spermine exposure on mutation induction before and after uv ir ... | 1984 | 6700607 |
| compared colonizing and immunizing efficiency of toxinogenic (a+ b+) vibrio cholerae and an a- b+ mutant (texas star-sr) studied in adult rabbits. | four strains of vibrio cholerae o1 were compared for their ability to colonize and immunize adult rabbit intestine. three were virulent, toxinogenic (a+ b+) isolates, and one, an a- b+ mutant (texas star-sr), was derived by mutagenesis with nitrosoguanidine. when given orally to nonimmune rabbits, virulent strains colonized the small bowel with similar efficiency, whereas texas star-sr colonized poorly. rabbits fed less than 50 cfu of an a+ b+ strain developed marked serotype-specific resistance ... | 1984 | 6715038 |
| glucose phosphoenolpyruvate-dependent phosphotransferase system of streptococcus mutans gs5 studied by using cell-free extracts. | the glucose phosphotransferase system (pts) of streptococcus mutans gs5 has been partially characterized, using fractions derived from cells treated with the muramidase mutanolysin. membranes retained functional pts enzymes for the phosphoenolpyruvate-dependent phosphorylation of glucose, fructose, and mannose. this was confirmed by assaying membranes directly for enzyme i (ei) and enzyme iiglc (eiiglc) by employing specific phosphoryl-exchange reactions for each factor. membranes prepared from ... | 1984 | 6715047 |
| [characteristics of long-term persisting strains of tick-borne encephalitis virus in different forms of the chronic process in animals]. | the properties of the vasilchenko strain of tick-borne encephalitis (tbe) virus and its 3 variants isolated at various stages of persistent infection (383, 453, and 535 days) in macaca rhesus monkeys and syrian hamsters with different forms of the chronic tbe were studied. the process characterized by chronic focal inflammatory-degenerative changes in the brains of hamsters without the disturbance of motor functions was associated with persistence of different kinds of virus-specific antigens wi ... | 1982 | 6753337 |
| [developmental cycle and the cytomorphological characteristics of the oleandomycin producer streptomyces antibioticus]. | the developmental cycle and cytomorphological features of the industrial strain ol-1 and its variant 0968 of the oleandomycin-producing organism were studied. variant 0968 was obtained as a result of exposure of the spores of strain ol-1 to uv light. when grown under submerged conditions in flasks with the rich medium, the strains were characterized by a complete developmental cycle consisting of three generations of the hyphae. every generation had a tendency for formation of submerged spores. ... | 1982 | 6756297 |
| pyomelanin production by pseudomonas aeruginosa. i. transformation of pyomelanin productivity. | pseudomonas aeruginosa was successfully transformed from a pyomelanin-producing strain to a non-pyomelanin-producing strain by genetic transformation, with an average frequency of 1.17 x 10-3/recipient. although the transformation frequency was not affected by doses of dna between 17 and 51 microgram/ml, it was influenced by the growth phase of the recipient bacteria, i.e., it was highest in the late logarithmic phase. biochemical functions of the transformants were the same as those of the reci ... | 1980 | 6767172 |
| mouse-protection experience with monovalent pseudomonas aeruginosa vaccine. | the protective effect of the soluble immunogenic complex from pseudomonas aeruginosa cell surface was studied on white mice. vaccines were prepared from a single strain by phenol-water extraction or via preparing the zn-complex. the immunogenic complex which contained the pseudomonas "common protective antigen" as well as endotoxin and exotoxins in the form of toxoid, provided reliable protection if it was administered with aluminium hydroxide as adjuvant in three consecutive doses of 10, 100 an ... | 1980 | 6775443 |
| immunological relationship between strains of theileria annulata dschunkowsky and luhs 1904. | the immunological relationship between the ludhiana and hissar strains, and between the uruli-kanchan, bangalore and jaipur strains of theileria annulata was studied. calves were immunised against the ludhiana strain by the infection-treatment method, against the uruli-kanchan strain by the infection-treatment method or by untreated low-grade infection and against the jaipur strain by untreated low-grade infection. after 45 days, groups of immune calves and susceptible controls were challenged w ... | 1980 | 6779361 |
| studies on the cross-resistance of mycobacterium tuberculosis, strain h37rv, to aminoglycoside- and peptide-antibiotics. | various phenotypes of the resistance to aminoglycoside- and peptide-antibodies of mycobacterium tuberculosis strain h37rv were produced by single- and/or two-step selection of the parent strain. mutants obtained by single-step selection with antibiotics were classified into ten phenotypes; one of single resistance, two of triple resistance, three of quadruple resistance, and four of sextuple resistance. there were two kinds of sextuple resistance (high resistance to enviomycin, viomycin, capreom ... | 1980 | 6783817 |
| inhibition of uridine 5'-diphosphate-n-acetylmuramyl-l-alanine synthetase by beta-chloro-l-alanine in escherichia coli. | the synthesis of the nucleotide precursors for peptidoglycan is regulated by the rela gene in escherichia coli. thus, nucleotide precursors labeled with [3h]diaminopimelic acid accumulated in a rela strain but not in an isogenic rela+ strain during amino acid deprivation. furthermore, nucleotide precursor synthesis was relaxed in the amino acid deprived rela+ strain by treatment with chloramphenicol. uridine diphosphate-n-acetylmuramyl-pentapeptide (udp-murnac-pentapeptide) was the major compone ... | 1982 | 6811120 |
| facile in vivo transfer of mutations between the bacillus subtilis chromosome and a plasmid harbouring homologous dna. | transformation of bacillus subtilis strains carrying the point mutations spoiia42 or spoiia69 by a plasmid, phm2, carrying the spoiia+ allele gave occasional recombinants in which the plasmid had acquired the chromosomal mutation. transformation of a spo+ strain by these derivative plasmids gave occasional recombinants in which the chromosome of the recipient had acquired the plasmid-borne spoiia mutation. both types of behaviour were rece+-dependent. mutations spoiia42 and spoiia69 fell into a ... | 1982 | 6818329 |
| [production of the mutant e strain of rickettsia prowazekii by mutagen exposure]. | the possibility of obtaining the mutants of r. prowazekii, strain e, by exposing these organisms to the action of n-methyl-n-nitro-n-nitrosoguanidine was studied; this substance, used in doses of 5-10 micrograms, showed a mutagenic effect on rickettsiae suspended in physiological saline, after their exposure for 10-20 minutes at 37 degrees c. the mutants thus obtained proved to be resistant to erythromycin and rifampicin and were characterized by heterogeneity in the degree and stability of thei ... | 1982 | 6818794 |
| synthesis and carcinogenic activity of oxidized benzacridines: potential metabolites of the strong carcinogen 7-methylbenz[c]acridine and of the inactive isomer 12-methylbenz[a]acridine. | the synthesis of 15 compounds related either to the benz[c]acridine or to the benz[a]acridine series is reported. spectral data, i.e., nmr and ei fragmentation, are given. these compounds were tested for carcinogenic activity in mice of the xviinc/z strain by subcutaneous injection. only three weak carcinogens were detected, 5,6-dihydro-5,6-dihydroxy-12-methylbenz[a]acridine, 3-methoxy-7-methylbenz[c]acridine, and 4-acetoxy-7-methylbenz[c]acridine. these results are discussed with consideration ... | 1983 | 6827550 |
| transferable resistance to clindamycin, erythromycin, and tetracycline in clostridium difficile. | resistance to clindamycin, erythromycin, and streptogramins in clostridium difficile could be transferred to a susceptible strain by mixed culture at low frequencies (1 x 10(-8) to 4 - 10(-8) per donor cell). transfer of tetracycline resistance occurred at frequencies of 3 x 10(-7) to 5 x 10(-7). no plasmid dna involved in these transfers could be detected. | 1983 | 6870225 |
| the development of a sex-linked slow-feathering white leghorn strain by gene transfer from an indian indigenous breed. | breeding techniques were used to transfer an economically important physiological character dependent on a single pair of alleles from an indigenous breed (kadakanath) to the white leghorn. the mating procedure used to develop a sex-linked, slow-feathering strain of white leghorn by these means is described and it is shown that using the simplest genetic and breeding techniques productivity can quickly be improved. | 1983 | 6883154 |
| isolation of bacterial luciferases by affinity chromatography on 2,2-diphenylpropylamine-sepharose: phosphate-mediated binding to an immobilized substrate analogue. | a covalently immobilized form of an inhibitor of bacterial luciferase, 2,2-diphenylpropylamine (d phi pa), was an effective affinity resin for purifying this enzyme from several distinct bacterial species. the inhibitor is competitive with the luciferase aldehyde substrate but enhances binding of the flavin substrate fmnh2 (reduced riboflavin 5'-phosphate); comparable binding interactions occur with luciferase, the immobilized inhibitor d phi pa-sepharose, and the substrates [holzman, t. f., & b ... | 1982 | 6983889 |
| analysis of outer membrane components of escherichia coli ml308 225 and of a serum-resistant mutant. | escherichia coli ml308 225 in the early exponential phase of batch cultures was killed by human serum in a complement-dependent reaction. under the same conditions, a mutant derived from this strain by multiple subcultures in human serum was resistant to killing by serum. the components of the outer membrane of both the parent and mutant strains were analyzed. the protein and phospholipid analyses were identical, but the mutant strain produced twice as much lipopolysaccharide as the parent strai ... | 1980 | 6995308 |
| antigenicity in hamsters of inactivated vaccines prepared from recombinant influenza viruses. | inactivated vaccines prepared form influenza virus strains obtained by the recombination of a/pr/8/34 (h1n1) or a/fm/1/47 (h1n1) viruses with a/victoria/3/75 (h3n2) virus, were tested for their antigenicity in hamsters. the parental origin of the genes of each cloned recombinant virus was determined by polyacrylamide gel electrophoresis, and vaccines prepared from each strain by concentration, purification on sucrose density gradients and inactivation with formalin. all the recombinant strains u ... | 1981 | 7031128 |
| treatment of acute myelogenous leukemia: influence of three induction regimens and maintenance with chemotherapy or bcg immunotherapy. | the effect of a synchronizing-recruiting drug schedule vs. myelotoxic therapy on remission rate and of bacillus calmette-guerin on remission duration and survival of adults with acute myelogenous leukemia were studied in a prospective cooperative trial. after randomized remission induction with arabinosyl cytosine + vincristine + methotrexate + leucovorin (avml), thioguanine + ara-c + daunorubin (tad), or daunorubicin + ara-c (da), complete remissions (cr) were consolidated with tad or avml. crs ... | 1982 | 7039813 |
| metabolic basis for the isoleucine, pantothenate or methionine requirement of ilvg strains of salmonella typhimurium. | salmonella typhimurium strain du501, which was found to be deficient in acetohydroxy acid synthase ii (ahas ii) and to possess elevated levels of transaminase b and biosynthetic threonine deaminase, required isoleucine, methionine, or pantothenate for growth. this strain accumulated alpha-ketobutyrate and, to a lesser extent, alpha-aminobutyrate. we found that alpha-ketobutyrate was a competitive substrate for ketopantoate hydroxymethyltransferase, the first enzyme in pantothenate biosynthesis. ... | 1982 | 7042686 |
| tetracycline-inducible transfer of tetracycline resistance in bacteroides fragilis in the absence of detectable plasmid dna. | tetracycline resistance of three bacteroides fragilis strains was shown to be inducible by subinhibitory concentrations of tetracycline. tetracycline resistance markers could be transferred to another b. fragilis strain by filter mating. the transferability was inducible by subinhibitory concentrations of tetracycline and did not take place in the absence of tetracycline. the optimum concentration of tetracycline for induction of transfer was about 2 microgram/ml. the transfer was shown to be a ... | 1982 | 7061390 |
| protection of mice from infection with streptococcus pneumoniae by anti-phosphocholine antibody. | anti-phosphocholine (pc) antibody mediated protection against many strains of streptococcus pneumoniae, and hybridoma anti-pc antibodies protected mice from fatal infections with types 1 and 3 s. pneumoniae. live types 1, 3, 5, 6a, and 19f s. pneumoniae had similar amounts of surface pc accessible to antibody. furthermore, mice expressing the x-linked immunodeficiency (xid) of the cba/n strain were found to be more susceptible to infection with s. pneumoniae of types 3, 6a, and 19f than were imm ... | 1982 | 7076292 |
| stress relaxation and creep behaviour of normal and carbon fibre reinforced acrylic bone cement. | room temperature compression creep and stress relaxation behaviour of normal as well as carbon fibre reinforced bone cement was studied and their properties compared. it was observed that increased deformation due to creep in 24 h was about 70% of the initial strain (due to applied constant stress fo 10.5 mn/m2) for normal pmma and this could be reduced to 45% of the initial strain by carbon fibre reinforcement. surgical grade pmma, when subjected to 1% constant strain, showed an average stress- ... | 1982 | 7082743 |
| [ergonomic strain measurement in text processing]. | text processing task demands are analysed by using an ergonomic job description questionnaire. the tasks are classified by cluster analysis. tasks out of each cluster are examined with respect to performance and physiological strain by 8-h measurements. these reactions are assessed in terms of tolerance limits and the distribution of demands is discussed for non-tolerable work conditions. | 1982 | 7129650 |
| estrogen and progesterone receptors in mammary tumors induced in rats by simultaneous administration of 17 beta-estradiol and progesterone. | mammary tumors were promoted in male rats of the wistar wag strain by continuous and simultaneous administration of 17 beta-estradiol and progesterone. tumor induction and growth were dependent on estradiol and on progesterone. their histological features were comparable with those of human breast cancers. hormone receptors were present in tumor cells. estradiol receptor was found in 95% of them, at a higher level in nuclei than in cytosol. progesterone receptor was present in 75% of tumors. in ... | 1982 | 7132354 |
| [identification of a new antigenic variant of simian adenovirus sa7 p]. | serological, biological, and physico-chemical properties of a new antigenic variant of simian adenovirus sa7p were studied. neutralization tests with hyperimmune specific antisera demonstrated the new antigenic variant sa7p to have very significant antigenic similarity with the prototype sa7 strain. same as the latter, sa7p does not agglutinate rat red blood cells, is highly oncogenic for newborn syrian hamsters and capable of transforming rat kidney cell cultures. at the same time, the method o ... | 1982 | 7135929 |
| serological differentiation of tobamoviruses by means of monoclonal antibodies. | several serologically related plant viruses have been differentiated for the first time by means of monoclonal antibodies. two orchid strains of tobacco mosaic virus that were previously considered to be serologically identical with the type strain were shown to be distinguishable with certain monoclonal antibodies. in indirect elisa, none of the antibodies showed any binding to tobamoviruses that differed from tmv (common strain) by a serological differentiation index larger than 2. monoclonal ... | 1982 | 7161367 |
| the influence of dietary magnesium on reduction of nephrocalcinosis in rats fed purified diet. | the severity of nephrocalcinosis in female colworth wistar rats fed on a standard purified diet was reduced by increasing the magnesium content of the diet; both its incidence and severity could be increased in males of the same strain by lowering the dietary magnesium level. | 1982 | 7176523 |
| comparative pulmonary tumorigenesis in dba/2j and c57bl/6j mice by administration of 3-methylcholanthrene and dimethylnitrosamine singly and combined. | c57bl/6j mice, which are inducible for both hepatic and pulmonary aryl hydrocarbon hydroxylase (ahh), and dba/2j mice, which are noninducible for hepatic ahh but moderately inducible for pulmonary ahh, received dimethylnitrosamine (dmn) i. p., or methylcholanthrene (mca) orally, or a combination of both agents, for 10 weeks; the animals were observed for an additional 26 weeks. the dosing schedule and total dose of dmn or mca or dmn + mca received were identical to those used by other investigat ... | 1982 | 7177243 |
| [effect of carbon and nitrogen sources on pullulan biosynthesis by polyploid strains of pullularia pullulans]. | the effect of eight carbohydrates and three nitrogen sources on the activity of pullulan accumulation was studied with a haploid strain, a diploid strain and a tetraploid strain of pullularia pullulans. the rate of pullulan biosynthesis was found to be the highest in media containing glucose, sucrose and maltose. in this case, the activity of the polysaccharide synthesis in the polyploid strains exceeded that in the haploid strain by 21-80% depending on the carbon source in the medium. the pullu ... | 2015 | 7194413 |
| evaluation of heat stress in a hot workshop by physiological measurements. | the use of physiological measurements, heart rate and oral temperature, for evaluating a heat stress situation in an industrial plant is described. in contrast to evaluation of hot jobs by environmental measurements and subjective evaluation of metabolic rate, the technique used with the wbgt criteria, evaluation of heat strain by measurements of the individual's heart rate and oral temperature is simpler, less expensive, less restrictive and more protective. a description of the technique invol ... | 1981 | 7223635 |
| [studies on the pheromonal pregnancy block in the mouse. ii. discriminatory and/or memorial mechanisms in female mice responding to the stud male]. | the present study was carried out to examine whether the stud male strain have a blocking effect on the pregnancy in stud female. four inbred mouse strains, c3h, c57bl/6, balb/c and cba, were used in these experiments. virgin female mice were caged together with a male of a different strain at 10-20 weeks of age for copulation. the mated females were removed from the male, and housed with a male strange or alien from the stud male. the "bruce effect" was evident in c3h, balb/c and cba strains, b ... | 1981 | 7262197 |
| suppression of inherent virulence for chickens of a newcastle disease strain by a shift within its subpopulations. | 1981 | 7271661 | |
| inhibition of intercellular communication between liver cells by the liver tumor promoter 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane. | a dose dependent inhibition of intercellular communication (metabolic cooperation) between primary cultures of rat liver hepatocytes and an established adult rat liver epithelial cell 6-thioguanine resistant strain by the liver tumor promoter 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (ddt) is demonstrated. this in vitro assay is proposed to evaluate the tumor promoting activity of oncogenic agents shown to be non-genotoxic in the liver culture systems. | 1981 | 7296527 |
| biosynthesis of nanaomycin. ii. purification and properties of nanaomycin d reductase involved in the formation of nanaomycin a from nanaomycin d1. | nanaomycin d reductase, catalyzing the conversion of nanaomycin d to nanaomycin a, which is the first step in the biosynthetic sequence (d leads to a leads to e leads to b) in streptomyces rosa var. notoensis, was purified from the crude extract of the strain by ammonium sulfate fractionation and column chromatography on deae-cellulose, sephadex g-100 and hydroxyapatite to give an electrophoretically homogeneous preparation. the enzyme was found to be a flavoprotein which contains fad as a prost ... | 1981 | 7298593 |
| characterization of abnormalities in the visual system of the mutant mouse pearl. | mice of the mutant strain pearl (pe/pe) differ from the wild strain by a single gene mutation, which leads to a lightening of the coat color. we tested this strain to see if this mutant gene also expressed itself in one or more visual abnormalities. pearl mice were found to lack totally the optokinetic nystagmus reflex that was present in every normal mouse that we examined. this lack of optokinetic nystagmus was not due to oculomotor defects, since postrotatory nystagmus was normal. as describe ... | 1981 | 7310489 |
| strain dependent effects of the enkephalin analogue fk 33-824 on locomotor activity in mice. | administration of the enkephalin analogue fk 33-824 was followed in the dba/2 (dba) strain by dose related depressant effects which, after the injection of 40 mg/kg, were still present 6 hrs after treatment; on the contrary, in the c57bl/6 (c57) strain, activity levels were enhanced by treatment, except for the dose of 40 mg/kg, which induced a short lasting behavioral stimulation followed by activity depression and catatonia and later on again by behavioral stimulation. all the effects of fk we ... | 1981 | 7312909 |
| rabies virus inactivation by binary ethylenimine: new method for inactivated vaccine production. | the inactivation dynamics of rabies virus (pv strain) by binary ethylenimine, and the immunogenic properites and the stability of the vaccines prepared using this agent, were studied. binary ethylenimine at a final concentration of 0.01 m was prepared wtih 2-bromoethylamine hydrobromide in alkaline solutions, either separately from or in suspensions of rabies virus propagated in bhk cells. the infectivity of virus suspensions containing more than 108 plaque-forming units per 0.1 ml was inactivat ... | 1980 | 7358836 |
| spontaneous variability of streptomyces glomeratus, a producer of the amthracycline antibiotics beromycins. | spontaneous variants of the beromycin-producing strain streptomyces glomeratus 3980 were divided into five groups (a-e) according to increasing antibiotic activity. the most active variants (group e) differed from the other types and the wild strain by a suppressed ability to produce aerial mycelium and melanoid piogment and by an increased production of propionic acid. strains with a 12-fold higher antibiotic production capacity (with respect to strain 3980) were obtained by selection of superi ... | 1980 | 7399371 |
| long-term torsional creep in compact bone. | bovine bone specimens loaded in torsion for periods of time exceeding one month exhibit pronounced time-dependent effects. the final strain exceeded the initial strain by a factor of more than four in some cases. creep failure was observed in two cases. the effects are not attributable to prolonged immersion in ringer's solution. | 1980 | 7412242 |
| experimental development of monensin resistance in eimeria meleagrimitis. | the discovery of monensin-resistant field isolates of eimeria meleagrimitis prompted an investigation of the ability of this parasite to develop monensin resistance in a laboratory selection experiment. a strain derived from a monensin-sensitive parent strain was intentionally selected for monensin resistance by propagation in monensin medicated turkeys in isolation, while the monensin-sensitive parent strain from which it was derived was cryogenically maintained as a genetic control. comparison ... | 1980 | 7413579 |
| biosynthesis of leupeptin. iii. isolation and properties of an enzyme synthesizing acetyl-l-leucine. | an enzyme which catalyzes acetylation of l-leucine with acetyl-coa was partially purified from a cell extract of streptomyces roseus ma839-a1, a leupeptin producer. the molecular weight of this enzyme is about 27,000 daltons. the enzyme has fairly broad specificity for acyl donors (i) and acceptors (ii): as for i, propionyl-coa was 1/10 as active as acetyl-coa with l-leucine as the acceptor; as for ii, l-leucyl-d-leucine, l-leucyl-l-leucine, l-arginine, l-leucyl-l-leucyl-l-leucine, l-phenylalani ... | 1980 | 7429989 |
| tumorigenic cell culture lines from a spontaneous vm/dk murine astrocytoma (sma). | one of the few spontaneous gliomas in inbred animals, the vm/dk spontaneous murine astrocytoma (sma), has seen limited use. previously restricted to an in vivo system, the sma was only transplantable intracerebrally (ic) using nonquantifiable suspensions of normal brain and tumor tissue. prior attempts at establishing permanent tumorigenic sma cell lines have not succeeded; tumorigenicity was lost during serial in vitro passage. we have established three different cell culture lines from a seria ... | 1980 | 7435141 |
| production of an attenuated influenza a (h3n2) strain "ko-1" by recombination with a/okuda/57 (h2n2) followed by selection of inhibitor-resistant virus. | a live attenuated influenza a (h3n2) strain "ko-1" was developed by recombination of wild influenza a/kumamoto/22/76 (h3n2) with attenuated a/okuda/57 (h2n2) followed by passage in developing chick eggs in the presence of horse serum inhibitor. the virus strain "ko-1" obtained is inhibitor-resistant, and has hemagglutinating (ha) and neuraminidase (na) antigenicity derived from the wild parent virus. rna analysis revealed that one rna segment (corresponding to m protein) was derived from the a/o ... | 1980 | 7437002 |
| in vitro differentiation of satellite cells isolated from normal and dystrophic mammalian muscles. a comparison with embryonic myogenic cells. | satellite cells were isolated from skeletal muscles of adult normal and dystrophic mice (c57/6j/dy strain) by sequential digestion of tissue fragments with collagenase, hyaluronidase and trypsin. these cells exhibit in culture similar behaviour to that of embryonic myoblasts, undergoing an initial duplicative period lasting about 2--3 days, followed by a shorter phase (1--2 days) of rapid cell fusion. during the duplicative phase most of the satellite cells appear round-shaped, whereas embryonic ... | 1980 | 7438216 |
| effect of immunization of guinea pigs on in vitro phagocytosis by polymorphonuclear leukocytes (pmnl). | it was found that the in vitro phagocytosis of 32p pulsed staphylococcus aureus 209 p strain by pmnl of immunized animals is inhibited in the presence of the antigens. the extent of inhibition depends on the antigen used. the release of acid phosphatase from phagocytizing pmnls decreased in the presence of antigens. | 1980 | 7447634 |
| comparison of mycoplasma synoviae hemagglutinating antigens by the hemagglutination-inhibition test. | mycoplasma synoviae (ms) strains were isolated from the trachea of hens from ms-positive broiler breeder flocks having progeny condemnations due to airsacculitis. hemagglutinating (ha) antigens were made from several strains. the ha antigen made from the 95th medium passage of ms fmt strain was compared with that of the standard ms wvu 1853 strain by the hemagglutination-inhibition (hi) test. sixty-six sera from 10 ms-positive flocks, 4 mycoplasma gallisepticum (mg)-positive sera, and 7 normal s ... | 1980 | 7447835 |
| x-linked dominant inheritance of partial phosphorylase kinase deficiency in mice. | a new mouse strain, the v strain, with a partial deficiency of phosphorylase kinase has been established. the deficiency is caused by an x-linked dominant gene (phkc). muscle extracts of homozygous and heterozygous females and hemizygous males have about 25% of the activity found in extracts of normal (c3h/hehan) mice. this dominant phosphorylase kinase deficiency of the new v strain is different from that of the i-strain mice with the x-linked recessive deficiency of skeletal muscle phosphoryla ... | 1980 | 7447922 |
| turkey breeder hen performance by strain during consecutive lay periods. | the results of this study provide information about reproductive performance among commercially available strains of large white turkey breeder hens, nicholas (n), british united (b), and hybrid (h). the hens were managed identically through two consecutive lay periods, summer-fall (1st-yr) followed by winter-spring (recycled). effects of the intensity (351 vs 24 lx) of supplemental light were evaluated during the 1st-yr lay period. data were collected for onset of oviposition, egg production, b ... | 1995 | 7479504 |
| [comparative study of the nucleotide sequence of the gene for the p/c wild strain or l-16 vaccine strain of measles virus]. | nucleotide sequences of p/c gene of measles virus il wild strain and l-16 vaccine strain were compared with the previously reported nucleotide sequence of edmonston vaccine strain p/c gene. the primary structure of il strain p/c gene was characterized by two substitutes in 395 (u-c) and 722 (g-a) positions in comparison with the primary structure of a similar edmonston strain. nucleotide sequence of l-16 vaccine strain p/c gene differed from the similar sequence of edmonston vaccine strain by 3 ... | 1995 | 7483563 |
| [induction of messenger rna of cytokines by herpes simplex virus infection in mice]. | the induction of the cytokine mrna after infection with herpes simplex virus (hsv) was studied using rt-pcr method capable of detecting low levels of mrna. total rna was prepared from spleen lymphocytes 3 h after infection with hsv-1 (+gc virulent variant and -gcr attenuated variant of miyama strain) by acid guanidinium thiocyanate-phenol-chloroform (agpc) method. cdna was synthesized by m-mlv reverse transcriptase, and amplified using the specific oligonucleotide primers for il-1, il-2, il-3, i ... | 1993 | 7517338 |
| cytokine sensitivity and methylation of lysine in rickettsia prowazekii evir and interferon-resistant r. prowazekii strains. | modified rickettsia prowazekii strains have been derived from the avirulent madrid e strain by passage in the lungs of white mice (strain evir) or by selection for resistance to gamma interferon (ifn-gamma) (strains 427-19 and 87-17) or alpha/beta interferon (ifn-alpha/beta) (strains 83-2p, 60p, 103-2p, and 110-1p). compared with the madrid e strain, strain evir has increased virulence (n. m. balayeva and v. n. nikolskaya, j. hyg. epidemiol. microbiol. immunol. 17:11-20, 1973) and a different ly ... | 1994 | 7518807 |
| antiatherosclerotic effects of the angiotensin-converting enzyme inhibitors captopril and fosinopril in hypercholesterolemic minipigs. | we evaluated the two angiotensin-converting enzyme (ace) inhibitors captopril and fosinopril with regard to possible antiatherosclerotic effects in minipigs. experimental hypercholesterolemia and atherosclerosis was produced in 33 minipigs of the göttingen strain by an egg yolk/cholesterol-enriched diet for 1 year. one group (n = 11) was fed the atherogenic diet alone and served as a control. a second group (n = 11) received captopril (80 mg/kg/day) added to the atherogenic diet, and a third gro ... | 1994 | 7528851 |
| analysis of reca mutants with altered sos functions. | the escherichia coli reca protein has at least three roles in sos mutagenesis: (1) derepression of the sos regulon by mediating lexa cleavage; (2) activation of the umud mutagenesis protein by mediating its cleavage; and (3) targeting the umu-like mutagenesis proteins to dna. using a combined approach of molecular and physiological assays, it is now possible to determine which of the three defined steps has been altered in any reca mutant. in this study, we have focused on the ability of six par ... | 1995 | 7528894 |
| occurrence and antigenic heterogeneity of l-2,4-diaminobutyrate decarboxylase in acinetobacter species. | we have previously reported that a novel enzyme, l-2,4-diaminobutyrate decarboxylase (daba dc), which is responsible for the formation of 1,3-diaminopropane, occurs in two acinetobacter species. the present study extends this observation to additional acinetobacter species and strains (6 reference strains and 30 clinical isolates). furthermore, the daba dc protein was detected in every strain by western blot analysis with the antiserum against the enzyme purified from a. baumannii atcc 19606. ho ... | 1995 | 7550102 |
| systemic infections in three infants due to a lactose-fermenting strain of salmonella virchow. | three previously healthy children developed gastroenteritis which led within a few days to systemic infections, two cases of bacteremia and one of meningitis. a lactose-fermenting salmonella virchow strain was isolated from cerebrospinal fluid and blood cultures. in one case, this strain was also isolated from stool cultures. all the children had been fed the same milk formula. there was no other relationship between them. the batch of dried-milk formula was confirmed as the source of the infect ... | 1995 | 7556238 |
| regulation of expression of the valine (branched-chain amino acid) dehydrogenase-encoding gene from streptomyces coelicolor. | expression of the streptomyces coelicolor (sc) valine (branched-chain amino acid) dehydrogenase-encoding gene (vdh) is regulated by valine, glucose and nh+4 at the transcriptional level. the results of assays for the level of accumulated vdh mrna in the sc j802 strain by primer extension experiments and for the level of catechol dioxygenase (xyle) activity in sc j802 (vdh::xyle) transformants show that transcription of the vdh gene is induced approx. 2.5-fold by valine, as compared to asparagine ... | 1995 | 7557420 |
| viral hazards to and from health care workers. | much attention has been directed towards nosocomial transmission of viruses as a result of clear evidence of patient-to-staff and staff-to-patient transmission of the blood-borne viruses hiv and hepatitis b virus. although the relatively long incubation periods of these viruses, together with the frequency of asymptomatic infections, renders problems for surveillance it has been possible to study modes of transmission and levels of risk over a number of years. information on trends of incidence ... | 1995 | 7560944 |
| a university outbreak of escherichia coli o157:h7 infections associated with roast beef and an unusually benign clinical course. | an outbreak of escherichia coli o157:h7 infections occurred after a graduation banquet at a university in wisconsin. sixty-one (32%) of 193 banquet attendees developed a gastrointestinal illness; 2 were hospitalized, none developed hemolytic-uremic syndrome or thrombotic thrombocytopenic purpura, and none died. the spectrum of illness was unusually mild, with 61% of ill persons reporting nonbloody diarrhea. a strain of e. coli o157:h7, indistinguishable from the outbreak strain by toxin type, pl ... | 1995 | 7561194 |
| peru-15, an improved live attenuated oral vaccine candidate for vibrio cholerae o1. | cholera vaccine candidate peru-15 was derived from a vibrio cholerae o1 el tor inaba strain by deleting the cholera toxin genetic element, introducing the gene encoding cholera toxin b subunit into reca, and screening for nonmotility. in a controlled study, peru-15 (2 x 10(8) cfu) was administered to 11 volunteers. no vaccinee developed diarrhea, and 10 of 11 had > 4-fold rises in vibriocidal antibody titers. one month later, 5 vaccinees and 5 control volunteers were challenged with wild type v. ... | 1995 | 7561195 |
| virulence of cryptococcus neoformans serotypes a, b, c and d for four mouse strains. | the relative virulence of cryptococcus neoformans serotypes a, b, c and d in four mouse strains was assessed by measuring their migration from the foot-pad of the animals to the spleen, lungs and brain in 6-week-old dba/2, balb/c, a/j and a hybrid mouse strain by re-isolating yeasts from the internal organs. comparable doses of each c. neoformans serotype were inoculated into the foot-pads of the mice. c. neoformans var neoformans strains a68, d52, a-(in) and d-(in) were more virulent than c. ne ... | 1995 | 7562991 |
| introduction of the transposable element minos into the germ line of drosophila melanogaster. | a transposon based on the transposable element minos from drosophila hydei was introduced into the genome of drosophila melanogaster using transformation mediated by the minos transposase. the transposon carries a wild-type version of the white gene (w) of drosophila inserted into the second exon of minos. transformation was obtained by injecting the transposon into preblastoderm embryos that were expressing transposase either from a hsp70-minos fusion inserted into the genome via p-element-medi ... | 1995 | 7568159 |
| prevalence and genetic location of non-transferable trimethoprim resistant dihydrofolate reductase genes in south african commensal faecal isolates. | in a recent survey of trimethoprim resistance, 357 gram-negative aerobic organisms were isolated from healthy volunteers from rural and urban populations in south africa. trimethoprim resistance did not transfer to an escherichia coli j62-2 recipient strain by conjugation in a liquid mating in 161 (45.1%) of the isolates. these isolates which did not transfer their resistance were probed with intragenic oligonucleotide probes for the types ia, ib, iiia, v, vi, vii, viii, ix, x and xii dihydrofol ... | 1995 | 7589265 |
| amidepsines, inhibitors of diacylglycerol acyltransferase produced by humicola sp. fo-2942. i. production, isolation and biological properties. | humicola sp. fo-2942, a soil isolate, was found to produce a series of new inhibitors of diacylglycerol acyltransferase (dgat). three active compounds, designated amidepsines a, b and c, were isolated from the fermentation broth of the producing strain by solvent extraction, silica gel column chromatography, ods column chromatography and hplc. amidepsines inhibit dgat activity with ic50 values of 10.2 approximately 51.6 microm in an enzyme assay system using rat liver microsomes. amidepsines als ... | 1995 | 7592058 |
| study of neutralizing monoclonal antibodies to bovine herpes virus type-1 (cooper strain) by immunoperoxidase and immunoelectron microscopy. | three neutralizing monoclonal antibodies (mabs) that are specific against bovine herpes virus type-1 (bhv-1) were studied as to their viral specificity by immunoperoxidase and immunoelectron microscopy. microscopic examination of gbk bhv-1 infected cells revealed peroxidase activity represented by red-brown granular deposits in the nucleus and cytoplasm. no immunoperoxidase activity was observed in negative controls. for the ultrastructural observations, two approaches were used. firstly we test ... | 1995 | 7599599 |
| transketolase a of escherichia coli k12. purification and properties of the enzyme from recombinant strains. | transketolase a was purified to apparent homogeneity from recombinant escherichia coli k12 cells carrying the homologous cloned tkta gene on a puc19-derived plasmid. these recombinant cells exhibited a transketolase activity in crude extracts of up to 9.7 u/mg compared to < or = 0.1 u/mg in wild-type cells. transketolase a was purified from crude extracts of a recombinant strain by successive ammonium sulfate precipitations and two anion-exchange chromatography steps (q-sepharose ff, fractogel e ... | 1995 | 7607225 |
| self-cloning in filamentous fungi: application to the construction of endothiapepsin overproducers in cryphonectria parasitica. | the filamentous ascomycete fungus cryphonectria parasitica naturally secretes endothiapepsin, an aspartic proteinase. it is cultured on a commercial scale as a source of the milk-clotting enzyme for cheese making. our objective was to increase enzyme production of an industrial c. parasitica strain by a new technique of self-cloning; it consisted in the screening for transformants producing higher levels of endothiapepsin and having integrated only the dna fragment of interest. such genetically ... | 1995 | 7612240 |
| incidence of prelay squatting behavior is not related to subsequent egg laying in turkey breeder hens. | sexual receptivity of turkey hens as indicated by squatting (sexual crouch) was evaluated in three strains of breeder hens, nicholas (n), hybrid (h), and british united (b). the incidence of squatting was compared among strains through 84 d of photostimulation and related to egg production characteristics. significant strain, time, and strain by time interaction effects occurred for the incidence of squatting. in all three strains, squatting started within the 1st wk of photostimulation, rapidly ... | 1995 | 7644415 |
| establishment of conditions for the detection of bovine herpesvirus-1 by polymerase chain reaction using primers in the thymidine kinase region. | polymerase chain reaction (pcr) for detection of bovine herpesvirus-1 (bhv-1) was developed and optimized using 22 bp sense and 20 bp antisense primers in the thymidine kinase (tk) coding region. the amplification product is 183 bp long. the pcr optimization was done using bhv-1 tissue culture supernate (bhv-1tcs), concentrated bhv-1 tissue culture supernate (cbhv-1tcs) and sucrose gradient purified bhv-1 (pbhv-1). the sensitivity of four methods of sample preparation which are standard dna extr ... | 1995 | 7648533 |
| comparison of four dna-based methods for strain delineation of candida lusitaniae. | four methods for the accurate delineation of epidemiologically related and unrelated strains of candida lusitaniae were compared. three pulsed-field electrophoretic methods, including two contour-clamped homogeneous field gel electrophoresis methods (ekp-1 and ekp-2) yielding electrophoretic karyotype patterns of intact chromosomal dna and a method in which the chromosomal dna was macrodigested with the endonuclease sfii prior to pulsed-field electrophoresis (mdp), and a random amplified polymor ... | 1995 | 7650168 |
| [changes in biogenesis and secretion of periplasmic alkaline phosphatase, coded by the phoa gene included in the escherichia coli dh1 plasmid]. | augmented synthesis of periplasmic alkaline phosphatase from e. coli dh1 coded by the phoa gene within the composition of plasmid phi-7 results in alterations in the enzyme biogenesis, such as accumulation of intermediate enzyme forms corresponding to various stages of its posttranslational modification, and alternative localization. the cell cytoplasm was found to contain a large proportion of alkaline phosphatase precursors in the form of insoluble aggregates. the mature enzyme was detected in ... | 1995 | 7662795 |
| isolation and sequencing of the replication region of mycobacterium avium plasmid plr7. | the mycobacterium avium plasmid plr7 is representative of a group of small plasmids that are common in isolates from aids patients with disseminated m. avium infections. determination of the functions of these and other plasmids has been hampered by the lack of methods for genetic manipulation of m. avium. in this study, the region of plr7 capable of replication was identified and sequenced. fragments of plr7 were cloned into a puc18 derivative carrying a kanamycin resistance marker and introduc ... | 1995 | 7665458 |
| cloning, sequencing, and characterization of the gene encoding the smallest subunit of the three-component membrane-bound alcohol dehydrogenase from acetobacter pasteurianus. | the membrane-bound alcohol dehydrogenase (adh) of acetobacter pasteurianus nci1452 consists of three different subunits, a 78-kda dehydrogenase subunit, a 48-kda cytochrome c subunit, and a 20-kda subunit of unknown function. for elucidation of the function of the smallest subunit, this gene was cloned from this strain by the oligonucleotide-probing method, and its nucleotide sequence was determined. comparison of the deduced amino acid sequence and the nh2-terminal sequence determined for the p ... | 1995 | 7665483 |
| stability of genomic dna fragment patterns in methicillin resistant staphylococcus aureus (mrsa) during the course of intra- and interhospital spread. | the analysis of genomic dna fragment patterns has revealed as a powerful tool for strain discrimination in staphylococcus aureus; for use as an epidemiological marker, stability during the course of an outbreak is an essential prerequisite. genomic dna fragment patterns (smai restriction, pulsed-field electrophoresis) of four different epidemic mrsa strains were compared along with intra- and interhospital and country-wide spread over more than 12 months in germany. strain i was isolated from in ... | 1994 | 7672057 |
| yeast seryl-trna synthetase expressed in escherichia coli recognizes bacterial serine-specific trnas in vivo. | the saccharomyces cerevisiae sers gene which encodes seryl-trna synthetase (serrs) was expressed in escherichia coli from the promoter and the ribosome binding sequences contained in its own 5'-flanking region. the low level of yeast serrs in the prokaryotic host was sufficient to permit in vivo complementation of two temperature-sensitive e. coli sers mutants at the nonpermissive temperature. thus, yeast serrs can aminoacylate e. coli trna(ser) species in vivo. yeast serrs, isolated from an ove ... | 1993 | 7686490 |
| functional properties of pro region of escherichia coli heat-stable enterotoxin. | escherichia coli heat-stable enterotoxin ip (stp) is synthesized as the 72-amino-acid residue precursor consisting of three regions: pre region (amino acid residues 1 to 19), pro region (amino acid residues 20 to 54), and mature st (mst) region (amino acid residues 55 to 72). we examined the role of the pro sequence of stp in enterotoxigenicity of a strain by deleting the gene fragment encoding amino acids 22 to 57. this deletion caused a remarkable reduction of its enterotoxic activity of cultu ... | 1993 | 7686611 |