Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular and immunochemical characterization of recombinant escherichia coli containing the spaa gene region of streptococcus sobrinus. | we identified and characterized a recombinant escherichia coli containing the entire gene for surface protein antigen a (spaa) of streptococcus sobrinus 6715. the recombinant e. coli was isolated from a cosmid gene bank of size-fractionated s. sobrinus dna fragments, and recombinants expressing the spaa protein were detected immunologically. subcloning experiments showed that the dna sequences encoding the spaa protein could be isolated on two contiguous ecori fragments, 3.7 and 3.3 kilobases (k ... | 1989 | 2543627 |
| modulation of the cellular immune responses to t-cell-dependent and t cell-independent antigens in lambs with induced bovine viral diarrhea virus infection. | functional interaction between lymphoid cells and lymphotropic viruses is particularly evident for bovine viral diarrhea virus (bvdv) in cattle and its closely related virus, the border disease virus (bvdv) in sheep. the most important aspect of acute or chronic phases of bvdv or bdv infection was the host's increased susceptibility to secondary bacterial or viral infection. to study the ability of bvdv to alter the development of the cellular immune responses to concomitant inoculation with t c ... | 1989 | 2552879 |
| [hypothermic effect of intracerebroventricular injections of taurine on endotoxin-induced fever in rabbits]. | taurine (tau) was infused by intracerebroventricular (icv) injection 20 min after iv endotoxin (et). cerebrospinal fluid was taken from the posterior cisterns at 0, 60, 210 and 390 min after tau infusion. the concentrations of camp and pge2 in the samples were determined by radioimmunoassay. in the control groups, an equivalent isotonic saline (ns) (non-pyrogenic) instead of tau or et was used respectively. the rectal and ear skin temperatures of the rabbits in a non-fasting state were recorded ... | 1989 | 2554667 |
| detection by antibody probes of human papillomavirus type 6 e5 proteins in respiratory papillomata. | we have demonstrated the expression of proteins arising from the e5a and e5b open reading frames (orfs) of human papillomavirus type 6c (hpv-6c) in respiratory tract papillomata. recombinant plasmids were constructed to express the orfs in the bacterial vectors path and prit2t. fusion proteins were purified and injected into rabbits to produce polyclonal antibodies. characterized antibodies generated against these fusion proteins were used in immunoperoxidase assays to identify the presence and ... | 1989 | 2559954 |
| production of diarrhea in the rabbit by a mutant of escherichia coli (rdec-1) that does not express adherence (af/r1) pili. | escherichia coli (rdec-1) adheres to peyer's patch and absorptive epithelium in the rabbit in the closely adhering manner characteristic of enteropathogenic and enterohemorrhagic e. coli. an adherence pilus (af/r1) is important for adherence to peyer's patch m cells in vivo and ileal brush borders in vitro. a nonpiliated mutant (42-2-37-8) of the rdec-1 strain colonized the gut lumen less readily than the parent strain. the mutant adhered infrequently to peyer's patch lymphoid follicle epitheliu ... | 1989 | 2567321 |
| bacterial cell surface hydrophobicity properties in the mediation of in vitro adhesion by the rabbit enteric pathogen escherichia coli strain rdec-1. | the role of hydrophobicity in the attachment of enteropathogens to gastrointestinal mucosa is controversial. in vitro binding of escherichia coli rdec-1 to rabbit intestine is dependent on the expression of pili. we examined in vitro adherence of piliated rdec-1 after altering either the hydrophobicity of the organisms, the hydrophobicity of the substrate for attachment, or the surface tension of the suspending liquid. hydrophobicity of rdec-1 was determined using four complementary methods. in ... | 1989 | 2572606 |
| gal-gal pyelonephritis escherichia coli pili linear immunogenic and antigenic epitopes. | the linear immunogenic and antigenic structure of e. coli gal-gal pili from the recombinant strain hu 849 was investigated with nine synthetic peptides corresponding to regions of the pilus sequence predicted to contain hydrophilic beta-turns. five peptides, as bovine serum albumin conjugates, were found by anti-hu 849 pilus serum and were thus designated "immunogenic epitopes." peptides corresponding to r 25-38, r 38-50, and r 48-61 (which jointly comprise the single intramolecular disulfide lo ... | 1985 | 2580037 |
| endotoxic activity and enterotoxigenicity of human strains of campylobacter jejuni isolated from patients in a nigerian hospital. | limulus gelation assay and dermal schwartzman reaction provided a sensitive and reproducible means of testing the endotoxic activity of hospital strains of campylobacter jejuni in lagos, nigeria. all the 22 isolates of campylobacter jejuni tested for the limulus gelation assay were positive for the production of endotoxin. furthermore, the campylobacter suspensions caused a positive dermal schwartzman reaction in rabbits. the area of skin reaction was less extensive than that produced by escheri ... | 1989 | 2632005 |
| molecular cloning and sequencing of the glycogen phosphorylase gene from escherichia coli. | the glgp gene, which codes for glycogen phosphorylase, was cloned from a genomic library of escherichia coli. the nucleotide sequence of the glgp gene contained a single open reading frame encoding a protein consisting of 790 amino acid residues. the glgp gene product, a polypeptide of mr 87,000, was confirmed by sds-polyacrylamide gel electrophoresis. the deduced amino acid sequence showed that homology between glgp of e. coli and rabbit glgp, human glgp, potato glgp, and e. coli malp was 48.6, ... | 1989 | 2645169 |
| molecular cloning and expression in escherichia coli k-12 of chromosomal genes determining the o7 lipopolysaccharide antigen of a human invasive strain of e. coli o7:k1. | we have cloned and studied the expression in escherichia coli k-12 of chromosomal rfb genes determining the biosynthesis of the o7 lipopolysaccharide (lps) antigen from e. coli k1 strain vw187. two e. coli k-12 strains carrying recombinant cosmids gave positive coagglutination reactions with protein a-rich staphylococcal particles bearing an o7-specific rabbit polyclonal antiserum. silver-stained polyacrylamide gels of total membranes extracted with hot phenol showed o side chain material which ... | 1989 | 2645215 |
| interaction of enteropathogenic escherichia coli 0111 with rabbit intestinal mucosa in vitro. | a model system using rabbit intestinal mucosal explants has been developed to examine the characteristic ultrastructural damage to the brush border induced by enteropathogenic escherichia coli 0111. in this model, as in others, bacterial adherence to the microvillous membranes occurred in two morphologically distinct stages. initial attachment of enteropathogenic strains of e. coli to ileal mucosa appeared to be a goblet cells and the mucous layer covering the microvilli. the next stage involved ... | 1989 | 2647575 |
| csf production in acute ventriculitis. | clinically, there appears to be a significant reduction in cerebrospinal fluid (csf) formation during acute ventriculitis--an observation that has not been well documented by experimental studies. to examine this phenomenon, an inoculum of escherichia coli was injected into the lateral ventricles of new zealand white rabbits. approximately 18 hours later, the survivors (64%) underwent a 3-hour ventriculocisternal perfusion of carbon-14-dextran (mw 7 x 10(4)) as a reference marker for csf formati ... | 1989 | 2647919 |
| expression of trypanosoma brucei procyclin as a fusion protein in escherichia coli. | procyclin, a glycoprotein surface antigen of procyclic forms of trypanosoma brucei, was expressed in escherichia coli as a cro-beta-galactosidase fusion protein. antibodies produced in rabbits immunised with gel-purified fusion protein bound to the surface of living procyclic culture forms in indirect immunofluorescence assays and were able to immunoprecipitate procyclin from lysates of trypanosomes biosynthetically labelled with tritiated proline. in addition, the antibodies recognised syntheti ... | 1989 | 2651916 |
| evaluation of the retrograde contamination guard in a bacteriologically challenged rabbit model. | we compared a newly developed closed urinary drainage system incorporating a retrograde contamination guard (rcg) with the conventional closed drainage system. the new system contains a solid bactericide (povidone iodine) pellet enclosed in a porous cartridge at the drain port of the urine collection bag. a catheterised rabbit model was used. the urine drainage bags were challenged daily for 8 days with an auxotrophically marked uropathogenic strain of escherichia coli at the outlet tube. the ba ... | 1989 | 2653556 |
| an enzyme immunoassay for human lymphotoxin. | a highly sensitive and specific enzyme immunoassay (eia) for human lymphotoxin (hlt) has been developed. the assay is based upon a sandwich system employing two kinds of anti-hlt antibodies with neutralizing activity. one of them was mouse monoclonal antibody raised against escherichia coli-derived recombinant hlt with a deletion of 20 amino-terminal amino acids and used as labelled antibody. the other was rabbit antibody raised against the carboxyl-terminal portion of hlt and used as solid-phas ... | 1989 | 2654187 |
| the primary structure of rabbit liver mitochondrial serine hydroxymethyltransferase. | the complete amino acid sequence of mitochondrial serine hydroxymethyltransferase from rabbit liver was determined. the sequence was obtained from analysis of peptides isolated from chymotryptic, cyanogen bromide, and limited acid cleavages of the protein. the enzyme consists of four identical subunits, each of 475 residues, i.e. 8 residues shorter than the subunit of the corresponding cytosolic isoenzyme. the sequences of the two rabbit proteins are easily aligned, provided a gap of 5 residues ... | 1989 | 2656682 |
| biotypes and o serogroups of escherichia coli involved in intestinal infections of weaned rabbits: clues to diagnosis of pathogenic strains. | a total of 575 escherichia coli strains isolated from weaned rabbits experiencing diarrhea in 119 french commercial farms were tested for o serogroups. the results showed a strong predominance of serogroup o103 strains. a sample of 126 strains were further checked for simplified biotypes by using five carbohydrate fermentation reactions. of 72 o103 strains, 70 were shown to belong to biotypes characterized by a rhamnose-negative reaction. four of nine serogroup o68 strains also showed this type ... | 1989 | 2656746 |
| shwartzman reaction in the brain induced by haemophilus somnus and escherichia coli lipopolysaccharide in rabbits. | intracerebral inoculation of viable haemophilus somnus resulted in suppurative or fibrino-suppurative meningitis of the brain and spinal cord in rabbits. multiple fibrin thrombosis complicated with meningitis in the central nervous system was produced by intracerebral inoculation of h. somnus followed by intravenous inoculation with escherichia coli lipopolysaccharide. the latter reaction may be attributable to a form of shwartzman reaction. | 1989 | 2656782 |
| effects of anti-lipid a human monoclonal antibody on lipopolysaccharide-induced toxicity to the kidney. | studies were done to evaluate the effects of the human monoclonal anti-lipid a igm antibody a6(h4c5) on several components of the hemodynamic and renal toxicity of the cell wall lipopolysaccharide of e. coli 0111:b4. antibody (0.25 to four mg./kg. bw) was administered 0.5 hour before, or premixed for one hour with, lipopolysaccharide (0.05 mg./kg., a 14 to 18% lethal dose), and the following measurements made over 0.5 to 3.5 hours of study: systemic arterial blood pressure, renal plasma flow, an ... | 1989 | 2657114 |
| cloning and expression of a cohemolysin, the camp factor of actinobacillus pleuropneumoniae. | the genetic determinant of the cohemolysin which is responsible for the camp phenomenon, a cohemolysis, of actinobacillus pleuropneumoniae was cloned in escherichia coli. total dna from the a. pleuropneumoniae serotype 1 type strain 4074 was used to construct a gene library in plasmid puc18 in e. coli jm83. a total of 10,500 clones containing recombinant plasmids have been screened for hemolysis on blood plates. fifty-five clones which showed a weak hemolytic response after 24 to 48 h of incubat ... | 1989 | 2659534 |
| synthesis of an enzymatically active flp recombinase in vitro: search for a dna-binding domain. | we have used an in vitro transcription and translation system to synthesize an enzymatically active flp protein. the flp mrna synthesized in vitro by sp6 polymerase is translated efficiently in a rabbit reticulocyte lysate to produce enzymatically active flp. using this system, we assessed the effect of deletions and tetrapeptide insertions on the ability of the respective variant proteins synthesized in vitro to bind to the flp recognition target site and to carry out excisive recombination. de ... | 1989 | 2664465 |
| [anaphylatoxin in the aqueous humor of rabbit with endotoxin induced endophthalmitis]. | anaphylatoxin-activated complement fragment is an important mediator of inflammation. in this study, trends of anaphylatoxin in clinical and experimental endophthalmitis was evaluated. the level of anaphylatoxin (c3a, c4a and c5a) and protein of human aqueous humor aspirated from patients with senile cataracts before surgery was measured. as a model of acute endophthalmitis, endotoxin-lipopolysaccharide from e. coli (lps) was used intravitreously in albino rabbits. the level of anaphylatoxin of ... | 1989 | 2665445 |
| fusion proteins containing androgen receptor sequences and their use in the production of poly- and monoclonal anti-androgen receptor antibodies. | complementary dna segments that encode different domains of human and rat androgen receptors were fused to the escherichia coli trpe gene using path expression vectors. fusion proteins expressed by the bacteria were used to immunize rats and rabbits to obtain polyclonal antibodies to androgen receptors. spleen cells of immunized rats were fused with myeloma cells to obtain stable hybridomas that produced monoclonal antibodies. gradient centrifugation and immuno-precipitation assays indicated tha ... | 1989 | 2666105 |
| purification of rabbit platelet secretory phospholipase a2 and its characteristics. | it was reported previously that rat platelets release phospholipase a2 upon in vitro stimulation by thrombin, adp, or a23187 (horigome, k., hayakawa, m., inoue, k., & nojima, s. (1987) j. biochem. 101, 53-61). secretion of phospholipase a2 was also observed with rabbit platelets. rabbit platelets seem to release phospholipase a2 upon stimulation in vivo, because the rabbit plasma taken immediately after intravenous injection of paf contained an appreciable level of phospholipase a2 activity and ... | 1989 | 2668261 |
| characterization of antibodies to dihydrothymine, a radiolysis product of dna. | antibodies to dihydrothymine were elicited by immunizing rabbits with dihydrothymidine monophosphate conjugated by carbodiimide to bsa. by use of an elisa assay, the antibodies produced were found to be specific for dihydrothymine. hapten inhibition studies showed that dihydrothymidine monophosphate was 3 orders of magnitude more effective as an inhibitor than thymidine monophosphate and 4 orders of magnitude more effective than thymidine glycol monophosphate. with dna containing dihydrothymine, ... | 1989 | 2669952 |
| the yopm gene of yersinia pestis encodes a released protein having homology with the human platelet surface protein gpib alpha. | in yersinia pestis kim, there are 11 yops (yersinial outer membrane proteins) encoded by the low-ca2+ response virulence plasmid pcd1. only yops m and n are found in easily detectable amounts in the culture medium. in this study, we located and characterized the yopm gene to obtain clues about its role in the virulence of y. pestis. rabbit antibody was raised against yops m and h, copurified from the supernatant of y. pseudotuberculosis 43(pgw600, pcd1 yope::mu di1[apr lac]). this antiserum was ... | 1989 | 2670888 |
| influence of endotoxin-induced fever on the pharmacokinetics of theophylline in the rabbit model. | to assess fever-induced changes in theophylline pharmacokinetics in the rabbit model, six healthy, male, new zealand white rabbits were studied using a randomized, matched-pair design. in treatment 1, 15 mg/kg of theophylline was infused into the left marginal ear vein, and several blood samples were collected from the opposite ear for 10 hours. treatment 2 was conducted in an identical manner, except 20 to 40 micrograms/kg of escherichia coli endotoxin was injected into the left marginal ear ve ... | 1989 | 2671956 |
| detection of conglutinin in bovine serum by complement-dependent agglutination of escherichia coli. | agglutination of escherichia coli (eca) by normal bovine serum was shown to be prevented by heating serum to 56 degrees c for 30 min, but restored by normal horse, swine, rabbit or guinea pig sera. further investigation of the eca reaction using techniques to distinguish between conglutination and immunoconglutination indicated eca to be a conglutination reaction. testing of 264 sera obtained from 22 normal cattle over a period of 5 months did not show individual or seasonal variation in eca. ch ... | 1989 | 2672554 |
| effect of steroids during escherichia coli endotoxinaemia in rabbits. | a study was carried out on the effect of high doses of methylprednisolone to rabbits in combination with or after challenging them with escherichia coli endotoxin. the animals given steroids simultaneously with the endotoxin or 2-6 h thereafter all had significantly higher white cell counts 24 h after endotoxin infusion. a second dose of endotoxin given 24 h after the first caused a rapid fall in white cell count, and no difference between the groups could then be observed 2 h later. the most st ... | 1989 | 2673951 |
| immunoserological comparison of 104-kilodalton proteins associated with hemolysis and cytolysis in actinobacillus pleuropneumoniae, actinobacillus suis, pasteurella haemolytica, and escherichia coli. | a homologous polyclonal antibody was produced in a rabbit to the 104-kilodalton (kda) protein hemolysin of actinobacillus pleuropneumoniae serotype 1 strain cm-5. in immunoblots, this antibody recognized a similar 104-kda protein produced in culture supernatants by a. pleuropneumoniae serotypes 1 to 12 and taxon "minor group" in addition to pasteurella haemolytica, actinobacillus suis, and alpha-hemolysin-producing escherichia coli (but only weakly in the latter two organisms). these results wer ... | 1989 | 2674017 |
| quantitation and properties of fecal and upper small intestinal aerobic microflora in infants and young children with persistent diarrhea. | the duodenal juice and fecal aerobic microflora was investigated in 54 patients with persistent diarrhea (age less than or equal to 2 years). the duodenal aspirates yielded increased aerobic bacteria (greater than 10(5) organisms/ml) in 28 (51.9%) of the patients. established enteric pathogens were isolated from the duodenal aspirates of 12 (22.2%) of the 54 patients, viz., enteroadherent escherichia coli (eaec) (5), enterotoxigenic e. coli (etec) (3), enteropathogenic e. coli (epec) (1), nontyp ... | 1989 | 2674383 |
| enzyme activation by yeast calmodulin and a calcium-binding protein of escherichia coli. | 1989 | 2678159 | |
| monoclonal antibodies for affinity purification of il-6/ifn- beta 2 and for neutralization of hgf activity. | two types of recombinant human il-6 (ril-6) were used for the development of specific monoclonal antibodies. the first was produced in e. coli and used for immunization, the second was produced in chinese hamster ovary cells (cho) and used for screening. the complete translated sequence of the cdna coding for human il-6 was fused, in phase, to protein-a and the hybrid gene was fused to the strong lambda pr promoter. this protein was purified from bacterial extracts by chromatography on rabbit ig ... | 1989 | 2680901 |
| fusion of genes encoding escherichia coli heat-stable enterotoxin and outer membrane protein ompc. | the ompc outer membrane protein of escherichia coli was used as a carrier molecule for the nonimmunogenic heat-stable enterotoxin sta. two fragments of different lengths of the gene encoding sta were fused in vitro to the 3' terminus of the truncated ompc gene. the resulting ompc-sta hybrid proteins could be detected by l-[35s]cysteine labeling, and they were processed and thus exported. all synthesized hybrid protein remained cell bound and was found by fractionation mainly in the periplasm. im ... | 1989 | 2680976 |
| extensive homology between the escherichia coli k-12 serc(pdxf) aminotransferase and a protein encoded by a progesterone-induced mrna in rabbit and human endometria. | 1989 | 2682527 | |
| glutaredoxin from rabbit bone marrow. purification, characterization, and amino acid sequence determined by tandem mass spectrometry. | a glutaredoxin was purified from rabbit bone marrow, and its amino acid sequence was determined by high performance tandem mass spectrometry. the sequences of peptides generated by digestion with trypsin alone or in combination with thermolysin were determined from their collision-induced dissociation (cid) mass spectra. alignment of these sequences and additional sequence information were obtained from the collision-induced dissociation mass spectra of peptides obtained from digestion of the in ... | 1989 | 2684977 |
| a sensitive enzyme immunoassay for human epidermal growth factor. determination of hegf in human serum and urine and pharmacokinetics in mouse. | a sensitive enzyme immunoassay for human epidermal growth factor (hegf) is described. the anti-hegf antibody was prepared by immunizing rabbits with hegf, which was synthesized by escherichia coli using the genetic engineering technique. the present assay system was based on the sandwiching of an antigen between anti-hegf f(ab')2 precoated on a 96-well polystyrene plate and beta-d-galactosidase-labeled anti-hegf fab'. the range of measurable hegf by this assay was 0.1-100 pg/well. recoveries of ... | 1989 | 2687456 |
| experimental studies on the pharmacokinetics and therapeutic effect of cefbuperazone in biliary infection. | a study was carried out using an experimental biliary infection model to investigate the pharmacokinetic characteristics and therapeutic effect of cefbuperazone in the rabbit. thirty rabbits were divided into three equal groups; a control group of normal animals, a group of infected animals receiving no cefbuperazone, and a group of infected animals receiving 50 mg cefbuperazone/kg intramuscularly. the experimental infection was made by direct inoculation of a suspension of e. coli into the comm ... | 1989 | 2692972 |
| cloning and expression of a trypomastigote-specific 85-kilodalton surface antigen gene from trypanosoma cruzi. | an 85-kda trypomastigote-specific surface antigen gene from trypanosoma cruzi has been identified by screening a genomic library in lambda gt10 with trypomastigote and epimastigote cdna. the 1.3-kb genomic clone (ptt34) hybridizes to a single trypomastigote mrna of 3.7 kb and to multiple bands in genomic southern blots. dot-blot experiments show that there are 5-10 copies of this sequence per haploid genome, and these are arranged in a non-tandem manner. ptt34 has been expressed as an anthranila ... | 1989 | 2693963 |
| expression and characterization of the tau subunit of phosphorylase kinase. | a cdna encoding the entire tau subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (cat) gene, respectively. a significant phosphorylase kinase activity was found, in the first case. in the second case, a fused protein containing 73 amino acids from the cat protein was obtained. after renaturation, t ... | 1989 | 2735920 |
| antibodies against an inter-domain segment of polypeptide chain inhibit active-site coupling in the pyruvate dehydrogenase multienzyme complex. | a synthetic peptide, aapaaapakqeaaapapaakaeapaaapaaka, proved to be an efficient and specific immunogen in rabbits. the amino acid sequence of the peptide is identical to that of the inter-domain region (pep3) linking the innermost of the three lipoyl domains to the dihydrolipoamide dehydrogenase-binding domain in the dihydrolipoamide acetyltransferase chain of the pyruvate dehydrogenase complex of escherichia coli. fab fragments from anti-pep3 antibodies selectively inhibited active-site coupli ... | 1989 | 2753138 |
| generation of polyclonal antibodies against purified rat whey acidic proteins and the synthesis of a tracer fusion protein suitable for use in radioimmunoassays. | rodent milk consists mainly of caseins and whey proteins. a major component of the latter group is the whey acidic proteins (wap) the gene for which has been cloned recently and shown to contain several potential glucocorticoid receptor binding sites. studies on the regulation of this gene by glucocorticoids would be greatly enhanced by the availability of a radioimmunoassay for wap. rat milk was obtained from lactating sprague-dawley rats and the wap purified to homogeneity by ammonium sulphate ... | 1989 | 2753223 |
| intestinal "normalization" of germ-free rabbits with rabbit caecal microflora: effect of dosing regimens. | hysterectomy-derived germ-free (gf) rabbits were given strictly anaerobic microflora obtained from the caecum of an antibiotic-decontaminated conventional rabbit. one group was given the caecal flora diluted in doe's milk. the second group received caecal flora without doe's milk and administration of the flora was repeated when the animals were given pelleted diet. body weight and intestinal parameters determined in the two groups of rabbits were compared with values in rabbits conventionally r ... | 1989 | 2756799 |
| rabbit il-1. cloning, expression, biologic properties, and transcription during endotoxemia. | the cloning, sequencing, expression, and biologic activities of rabbit il-1 alpha and beta are described. a cdna library was constructed in lambda gt10 by using polyadenylated rna extracted from rabbit adherent splenic macrophages 4 h after stimulation with endotoxin. by using the cdna for human il-1 beta and il-1 alpha as hybridization probes, cdna for both forms of rabbit il-1 were isolated. the cdna for rabbit il-1 beta encodes a precursor polypeptide of 268 amino acids with an overall homolo ... | 1989 | 2784458 |
| the effect of vitamin d3 on the production of cytotoxic factors by human peripheral blood mononuclear cells. | vitamin d3 at a concentration of 300 nm was shown to significantly reduce the yields of cytotoxin produced by human peripheral blood mononuclear cells. reduction of cytotoxin titers occurred when induction was performed with either concanavalin a, interleukin 2, or ionomycin. typing of this cytotoxin was accomplished using antisera that were prepared by immunization of rabbits with either recombinant tumor necrosis factor or lymphotoxin. both antisera were demonstrated to neutralize the cytotoxi ... | 1989 | 2788495 |
| high levels of circulating neutralizing antibody in normal animals to recombinant mouse interferon-beta produced in yeast. | plasma from normal outbred swiss mice not previously given interferon (ifn) neutralized a glycosylated (high-mannose) recombinant murine ifn-beta made in yeast (rmuifn-beta y). the neutralization antibody titers were as high as 1:6,000 versus rmuifn-beta y, whereas the titers obtained with native murine ifn-beta (muifn-beta) were 100-fold less; a recombinant murine ifn-beta make in escherichia coli (rmuifn-beta ec) was not neutralized at 1:10 dilution of plasma. an elisa using rmuifn-beta y demo ... | 1989 | 2809279 |
| cloning and expression of the salmonella enterotoxin gene. | this report examines the genetic basis for salmonella typhimurium q1 enterotoxin production. a 918-base-pair xbai-hincii fragment of plasmid pjm17, composed of cholera toxin (ct) coding sequences (ctxab), was used as a gene probe. with this probe, the s. typhimurium enterotoxin was identified on a 6.3-kilobase ecori-psti fragment of chromosomal dna from plasmidless strain q1. we cloned this 6.3-kilobase fragment into escherichia coli rr1. the genetic map of the cloned salmonella enterotoxin (stx ... | 1987 | 2822664 |
| cruciform extrusion in plasmids bearing the replicative intermediate configuration of a poxvirus telomere. | the transition from lineform dna to cruciform dna (cruciformation) within the cloned telomere sequences of the leporipoxvirus shope fibroma virus (sfv) has been studied. the viral telomere sequences have been cloned in recombination-deficient escherichia coli as a 322 base-pair, imperfect palindromic insert in puc13. the inverted repeat configuration is equivalent to the arrangement of the telomere structures observed within viral dna replicative intermediates. a major cruciform structure in the ... | 1987 | 2824785 |
| cloning and expression in escherichia coli of the clostridium thermoaceticum gene encoding thermostable formyltetrahydrofolate synthetase. | formyltetrahydrofolate synthetase (fthfs) (ec 6.3.4.3), a thermostable protein of four identical subunits from clostridium thermoaceticum was cloned into escherichia coli sk1592. the clone (crl47) contained a 9.5 kb ecori fragment of c. thermoaceticum dna ligated into pbr322. it produced catalytically active, thermostable fthfs, that was not found in e. coli sk1592 containing native pbr322. the identity of the expressed enzyme was confirmed by specific binding of rabbit polyclonal anti-fthfs ser ... | 1988 | 2833195 |
| conservation of primary structure in the lipoyl-bearing and dihydrolipoyl dehydrogenase binding domains of mammalian branched-chain alpha-keto acid dehydrogenase complex: molecular cloning of human and bovine transacylase (e2) cdnas. | the subunit structures and conservation of the dihydrolipoyl transacylase (e2) components of bovine and human branched-chain alpha-keto acid dehydrogenase complexes were investigated by western blotting, peptide sequencing, and cdna cloning methods. rabbit antiserum prepared against the sodium dodecyl sulfate (sds) denaturated bovine e2 subunit recognized the inner e2 core, and the first hinge region of the e2 chain, but failed to react with the lipoyl-bearing domain as determined by western blo ... | 1988 | 2837277 |
| inorganic pyrophosphate: fructose-6-phosphate 1-phosphotransferase of the potato tuber is related to the major atp-dependent phosphofructokinase of e. coli. | a procedure was developed for the purification of inorganic pyrophosphate: fructose-6-phosphate 1-phospho-transferase (ppi-pfk) from potato tubers. the enzyme has the structure alpha 4 beta 4 with a subunit of 68 kda and a beta subunit of 60 kda. the structural relationship of this enzyme to other pfks and to fructose bisphosphatase was examined by immunoprecipitation and immunoblotting. antibodies to the plant enzyme did not react with e. coli pfk. no cross-reaction was seen among the following ... | 1988 | 2840062 |
| expression of coxsackievirus b3 capsid proteins in escherichia coli and generation of virus-specific antisera. | subgenomic fragments of cloned infectious coxsackievirus b3 (cvb3) cdna up to the size of the complete coding sequence of the viral polyprotein were inserted into the prokaryotic expression vector pplc24 and expressed in escherichia coli. fusion proteins, containing 54 amino acids of ms2 replicase at their amino terminus followed by different parts of the cvb3 structural proteins, were expressed from several constructs. the expression product of a plasmid encoding the capsid proteins vp4, vp2, a ... | 1988 | 2841081 |
| enzyme binding-inhibiting assay for iodothyronine 5'-monodeiodinase (5'-md) and its application to isolation of complementary deoxyribonucleic acid clones for the 5'-md in rat liver. | to identify and/or quantify type i t4 5'-monodeiodinase (5'-md) immunologically, polyclonal antibodies were produced by immunization of rabbits with solubilized microsomal proteins (smp) from rat liver. pilot studies showed that the antibody binds to, but does not neutralize, rat liver enzyme. we have employed the polyclonal antibody to develop a 5'-md enzyme binding-inhibiting assay (mbia). for this purpose, active, inactive, or synthetic 5'-md was preincubated with rabbit antibody and removed ... | 1988 | 2841089 |
| studies on the mechanism of translational enhancement by the 5'-leader sequence of tobacco mosaic virus rna. | translation of foreign mrnas is enhanced by a cis-acting derivative (omega') of the 5'-leader sequence (omega) of tobacco mosaic virus rna (vulgare strain). to explain this effect we have conducted several experiments in vitro. 1. the presence of various 5'-terminal sequences, including omega', did not significantly increase the half-lives of chloramphenicol acetyltransferase (cat) or neomycin phosphotransferase (nptii) mrnas in wheat-germ extract. also, a long leader sequence, unrelated to omeg ... | 1988 | 2841127 |
| expression of herpes simplex virus type 1 dna polymerase in saccharomyces cerevisiae and detection of virus-specific enzyme activity in cell-free lysates. | the herpes simplex virus type 1 (hsv-1) (strain 17) dna polymerase gene has been cloned into an escherichia coli-yeast shuttle vector fused to the galactokinase gene (gal-1) promoter. genes controlled by the gal-1 promoter are induced by galactose, uninduced by raffinose, and repressed by glucose. cell extracts from a strain of saccharomyces cerevisiae harboring this vector (y-mh202, expresser cells) grown in the presence of galactose and assayed in high salt (100 mm ammonium sulfate) contained ... | 1988 | 2846866 |
| use of retroviral vectors for mapping of splice sites in cottontail rabbit papillomavirus. | cottontail rabbit papillomavirus (crpv) genomic sequences coding for virus early functions were introduced into a retroviral vector in order to produce cdnas of the viral early region. two constructs differing in the length of control sequences preceding the e6 open reading frame were transfected into psi-2 cells and the released retroviral stock was used to infect nih3t3 cells. the proviral sequences were rescued from antibiotic g418-resistant virus-infected cells after fusion with cos cells, a ... | 1988 | 2848927 |
| a new heat-labile cytolethal distending toxin (cldt) produced by escherichia coli isolates from clinical material. | a new heat-labile escherichia coli toxin cytolethal to vero, hela, hep-2 and cho cells and negative in y-1 cells has been demonstrated in culture filtrates of 43 e. coli strains associated with diarrheal disease. this new toxin was termed a cytolethal distending toxin (cldt) to reflect the progressive cell distention and cytotoxicity evidenced in all sensitive tissue cells. cldt was distinct from the classic heat-labile (lt) and heat-stable enterotoxins, verotoxins and hemolysins and was produce ... | 1988 | 2849027 |
| mode of acth antipyretic action. | the method of intestinal cooling was used to analyze the effect of centrally administered acth in microgram quantities on hypothalamic centers regulating activity of thermoregulatory outputs (cold thermogenesis--ct, peripheral vasomotor tone--pvmt, respiratory evaporative heat loss--rehl). acth, when injected into the supraoptic area of the anterior hypothalamus of normal rabbits, had no significant effect on body temperature control. intrahypothalamic administration of acth during the early pha ... | 1988 | 2850094 |
| versatile plasmid vectors for use in studies of eukaryotic gene expression. | a new pair of plasmid vectors useful in transient expression experiments of genes from higher eukaryotes was constructed. the vectors have been developed as derivatives of pseg, a cloning and expression vector that has been used in studies of gene promoter structure and function [barrera-saldaña et al., embo j. 4 (1985) 3839-3849]. puanl1 and puanl2 include in their configuration a complete rabbit beta-globin transcriptional unit as internal control for gene expression the simian virus 40 (sv40) ... | 1988 | 2850973 |
| purification and characterization of a receptor for the 987p pilus of escherichia coli. | a receptor-containing fraction that caused visible aggregation of piliated escherichia coli strain 987 bacteria was released into solution when brush borders from adult rabbit small intestines were stored. the receptor-containing fraction was separated into more than 50 bands by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 4 of which contained carbohydrate as demonstrated by staining with periodic acid-schiff. receptor activity in the receptor-containing fraction was associated wit ... | 1985 | 2856917 |
| bacterial adherence. | 1985 | 2858808 | |
| inactivation of glutamine synthetase by a purified rabbit liver microsomal cytochrome p-450 system. | several mixed-function oxidation systems catalyze inactivation of escherichia coli glutamine synthetase and other key metabolic enzymes. in the presence of nadph and molecular oxygen, highly purified preparations of cytochrome p-450 reductase and cytochrome p-450 (isozyme 2) from rabbit liver microsomes catalyze enzyme inactivation. the inactivation reaction is stimulated by fe(iii) or cu(ii) and is inhibited by catalase, mn(ii), zn(ii), histidine, and the metal chelators o-phenanthroline and ed ... | 1985 | 2861789 |
| expression of human terminal deoxynucleotidyl transferase in escherichia coli. | a cloned dna fragment related to pt17 containing a partial cdna sequence of human terminal deoxynucleotidyl transferase was used as a probe to screen for the full length cdna sequence of the enzyme in a lambda gt11 library constructed from human lymphoblastoid km-3 cdna. a recombinant containing a 2068-base pair insert was isolated and recloned into the ecori site of the sequencing plasmic puc-8 as two subclones, pt711 and pt106. dna sequencing and hybridization studies showed that pt711 contain ... | 1985 | 2863268 |
| import and processing of hybrid proteins by mammalian mitochondria in vitro. | normal and hybrid proteins were synthesized following transcription of psp64 recombinant plasmids with sp6 polymerase and translation of resultant mrnas in a rabbit reticulocyte lysate. the precursor to the rat liver mitochondrial matrix enzyme, ornithine carbamyltransferase (mr = approximately 40,000), was efficiently imported by rat heart mitochondria in vitro and processed to mature protein. import of the precursor to a second matrix enzyme, carbamyl-phosphate synthetase i (mr = approximately ... | 1986 | 2867093 |
| gene products specifying adhesion of uropathogenic escherichia coli are minor components of pili. | the pape, papf, and papg genes of uropathogenic escherichia coli are dispensable for the synthesis and assembly of pili associated with pyelonephritis, called pap pili. phenotypically, papf and papg mediate digalactoside [alpha-d-galp-(1----4)-beta-d-galp)-specific adhesion. although whole bacterial cells of a pape mutant bind to this receptor, purified pili from such a mutant do not. this is in contrast to pili purified from the wild type, which bind specifically. the dna sequences of the pape ... | 1986 | 2869489 |
| characterization of escherichia coli wild-type strains by means of agglutination with antisera raised against cloned p-, s-, and ms-fimbriae antigens, hemagglutination, serotyping and hemolysin production. | e. coli strains isolated from patients with urinary tract infections (uti) very often possess mannose-sensitive (ms) and mannose-resistant (mr) adherence factors (fimbriae). according to their receptor specificity the mannose-resistant adhesins can be divided into several types, p, s, m and x. we have cloned the determinants of three groups of uti e. coli adhesins, ms, p and s, and prepared specific antisera against the fimbriae antigens. 189 hemagglutination (ha+)-positive strains, 96 fecal iso ... | 1986 | 2874672 |
| non-haemagglutinating fimbriae of enteropathogenic escherichia coli (epec). | two hundred and thirteen escherichia coli strains originating in 12 countries were included in the study. of these, 157 were classical enteropathogenic e. coli (epec) serotypes, 54 belonged to o138, o139 and o141 serogroups i.e. porcine edema disease strains and two strains were of serogroup o157 associated with haemorrhagic colitis. surface hydrophobicity was determined by the salt aggregation test (sat). haemagglutination was assayed against erythrocytes of six animal species with strains grow ... | 1986 | 2876564 |
| primary structure of the neurospora plasma membrane h+-atpase deduced from the gene sequence. homology to na+/k+-, ca2+-, and k+-atpase. | the gene for the neurospora crassa plasma membrane h+-atpase has been cloned and sequenced. the gene encodes for a protein of 920 amino acids with a molecular weight of 100,002. the coding region is interrupted by four introns: three near the amino terminus and one near the carboxyl terminus. the deduced amino acid sequence of the n. crassa plasma membrane h+-atpase exhibits 75% homology to the amino acid sequence of the saccharomyces cerevisiae plasma membrane h+-atpase. also, an amino acid com ... | 1986 | 2876992 |
| purification, morphology, and genetics of a new fimbrial putative colonization factor of enterotoxigenic escherichia coli o159:h4. | the ability to colonize the small intestine is essential for the pathogenesis of diarrhea caused by enterotoxigenic escherichia coli (etec). colonization is mediated by fimbriae (pili), of which there are several antigenically distinct types, including colonization factor antigen i, colonization factor antigen ii (cs1, cs2, and cs3), and pcf8775 (cs4, cs5, and cs6). these fimbriae are associated with certain etec o serogroups. serogroup o159 has had no known colonization factor. we found a disti ... | 1987 | 2883122 |
| demonstration by immuno-electronmicroscopy of antigenic heterogeneity among p fimbriae of strains of escherichia coli. | the identification of p fimbriae on urinary strains of escherichia coli isolated from urine was made by observation of the patterns of mannose-resistant and eluting haemagglutination of erythrocytes of seven animal species (and including those of human p phenotype), and by haemagglutination-inhibition tests with hydatid-cyst fluid known to contain an analogue of p-fimbrial receptor. in tests with five different, pure p-fimbrial antisera prepared in rabbits, agglutinin titres of 37 p-fimbriate st ... | 1987 | 2884321 |
| surface properties of the vero cytotoxin-producing escherichia coli o157:h7. | strains of escherichia coli serotype o157:h7 are vero cytotoxin-producing enteric pathogens which have been associated with sporadic cases and outbreaks of hemorrhagic colitis and with the hemolytic uremic syndrome in humans. in addition to toxin production, adherence of many pathogenic bacteria to intestinal mucosal surfaces is a critical primary step in the pathogenesis of diarrheal diseases. although e. coli serotype o157:h7 organisms adhere to intestinal epithelia of orally infected animals ... | 1987 | 2886431 |
| intragastric inoculation with an e. coli pilus attachment factor (af/r1) protects against subsequent colonization by an enteropathogen (e. coli strain rdec-1). | 1987 | 2892360 | |
| protective antigens against enterotoxigenic escherichia coli o101:k99,f41 in the infant mouse diarrhea model. | protective antigens present in whole cells of bovine enterotoxigenic escherichia coli strains were tested in an infant mouse diarrhea model. reduction of mortality rates of infant mice suckling vaccinated mothers was measured 1, 2, and 5 days after oral challenge with strain b41 (o101:k99,f41:h-). vaccines consisted of strains of serogroup o101, o9, o8, or o20 and of variants bearing or not bearing antigens k99 and f41. k99 and f41 expressions were checked by slide agglutination with k99 and f41 ... | 1988 | 2895746 |
| role of type 1 somatic pili (fimbriae) in mucosal attachment of the enteroadherent escherichia coli, strain rdec-1, in rabbits. | we have previously shown that attachment of the rabbit enteroadherent escherichia coli, strain rdec-1, to ileal brush borders in vitro is mediated by both mannose-resistant, af/r1 pili and mannose-sensitive, type 1 pili. because the role of type 1, somatic pili as adhesins that mediate bacterial enteroadherence in vivo remains controversial, we examined adherence of rdec-1 expressing either type 1 pili or af/r1 pili to rabbit ileum in ligated loops and after oral infection of rabbits. a rabbit f ... | 1988 | 2899622 |
| cloning and nucleotide sequence of a gene for actinomyces naeslundii wvu45 type 2 fimbriae. | a genomic library of actinomyces naeslundii wvu45 dna in escherichia coli was screened for antigen expression with rabbit antibody against a. naeslundii fimbriae. western blotting (immunoblotting) of one recombinant clone carrying a 13.8-kilobase-pair insert revealed a 59-kilodalton (kda) immunoreactive protein. a protein of similar electrophoretic mobility was detected from the isolated fimbrial antigen. expression of the 59-kda cloned protein in e. coli was directed by a promoter from the inse ... | 1988 | 2900829 |
| isolation and characterization of the localized adherence factor of enteropathogenic escherichia coli. | the binding factor of enteropathogenic escherichia coli o111:h- responsible for localized adherence (la) on hela cells was investigated. inhibition of la by carbohydrates and lectins showed that the reactive epitope on hela cells contains n-acetylgalactosamine units. treatment of bacteria with edta for extraction of lipopolysaccharides eliminated these polymers as binding factors. such treatment also caused a marked increase in adhesion suggesting steric hindrance by lipopolysaccharides of the l ... | 1988 | 2902011 |
| expression and secretion of pertussis toxin subunit s1 in bacillus subtilis. | pertussis toxin (pt) is an important virulence determinant of bordetella pertussis and one of the major protective antigens against whooping cough. the genes coding for pt have recently been cloned, but attempts to express them in escherichia coli have been unsuccessful. we therefore explored the possibility of expressing these genes in bacilius subtilis for which efficient vectors are available. the lack of endotoxin in the gram-positive bacillus might be an additional advantage for the product ... | 1987 | 2904640 |
| substrate specificity and inhibitors of a capillary injury-related protease from sheep lung lymph. | a serine protease (mr 70,000 to 75,000) appearing in sheep lung lymph after capillary damage induced by escherichia coli endotoxin, oleic acid, or air emboli, was studied for its specificity toward a series of synthetic peptide and thioester substrates containing an arg residue in the p1 position. high specificity constants (kcat/km) were generally obtained with substrates having two or more basic amino acid residues, and with those having a gln residues in the p2 position. secondary enzyme-subs ... | 1989 | 2916836 |
| the developmentally regulated p100/11e gene of leishmania major shows homology to a superfamily of reductase genes. | the life cycle transformation of the protozoan parasite leishmania from promastigote to amastigote is accompanied by changes in the level of expression of a number of proteins whose function may be necessary for parasite survival in the sandfly vector or mammalian host. to genetically characterize these proteins, we have cloned and characterized cdna sequences that vary in abundance during the life cycle of leishmania major. one sequence (p100/11e) encodes a poly(a+) rna whose abundance is marke ... | 1989 | 2918000 |
| recombinant protein a of non-staphylococcal origin is not mitogenic for human peripheral lymphocytes. mitogenicity of natural protein a is caused by a contaminant. | recombinant protein a(spa) produced in escherichia coli or bacillus subtilis bacteria did not induce activation of human peripheral mononuclear cells, whereas spa preparations obtained from naturally occurring staphylococcus aureus bacteria as well as recombinant spa from staphylococcus xylosus were potent mitogens. further purification of spa from s. aureus showed that the mitogenic material was concentrated in the side fractions containing more basic molecules. some staphylococcal enterotoxins ... | 1989 | 2922571 |
| characteristics of toxic shock syndrome toxin 1-induced lectin-like activity and inhibitor(s) in rabbit serum. | in adult rabbits intravenously injected with toxic shock syndrome toxin 1 (tsst-1) or staphylococcal enterotoxin b, serum lectin-like activity (detectable by cation-dependent agglutination of bacteria) developed. this activity was sensitive to heat, trypsin, and formaldehyde but resistant to neuraminidase or galactose oxidase. formaldehyde-fixed propionibacterium acnes or escherichia coli cells reactive with plant lectins provided sensitive targets for titration of serum agglutination activity t ... | 1989 | 2928648 |
| [histopathological changes in the organs of rabbits with experimental shock induced by an endotoxin of e. coli]. | 1986 | 2946448 | |
| local shwartzman activity of lipopolysaccharides from several selected strains of suspected periodontopathic bacteria. | 1987 | 2953879 | |
| purification and characterization of a lipocortin-like 33 kda protein from guinea pig neutrophils. | a lipocortin-like, phospholipase a2 inhibitory 33 kda protein was purified from guinea pig neutrophils. from amino acid composition and sequence data, this protein was found to have a high degree of homology to human lipocortin i. this protein inhibited porcine pancreatic phospholipase a2 activity in the presence of [3h]oleic acid-labeled escherichia coli membranes as substrate. maximal inhibition amounted to 65% whereas 50% inhibition occurred at 83.5 nm. this protein showed f-actin-binding abi ... | 1988 | 2962884 |
| the structure of the amino terminus of tropomyosin is critical for binding to actin in the absence and presence of troponin. | analysis of two recombinant variants of chicken striated muscle alpha-tropomyosin has shown that the structure of the amino terminus is crucial for most aspects of tropomyosin function: affinity to actin, promotion of binding to actin by troponin, and regulation of the actomyosin mgatpase. initial characterization of variants expressed and isolated from escherichia coli has been published (hitchcock-degregori, s. e., and heald, r. w. (1987) j. biol. chem. 262, 9730-9735). fusion tropomyosin cont ... | 1988 | 2965699 |
| [epidemiologic role of lactose-positive enterotoxigenic e. coli]. | 1988 | 2969296 | |
| analysis of the escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes. | the nucleotide sequences of the escherichia coli genome between the glycogen biosynthetic genes glgb and glgc, and 1170 bp of dna which follows glga have been determined. the region between glgb and glgc contains an open reading frame (orf) of 1521 bp which we call glgx. this orf is capable of coding for an mr 56,684 protein. the deduced amino acid (aa) sequence for the putative product shows significant similarity to the e. coli glycogen branching enzyme, and to several different glucan hydrola ... | 1988 | 2975249 |
| highly specific antibody to rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules. | antiserum to the rous sarcoma virus (rsv)-transforming protein, pp60v-src, was produced in rabbits immunized with p60 expressed in escherichia coli. alpha p60 serum immunoprecipitated quantitatively more pp60v-src than did tumor-bearing rabbit (tbr) sera. when rsv-transformed cell lysates were preadsorbed with tbr serum, the remaining lysate contained additional pp60v-src, which was recognized only by reimmunoprecipitation with alpha p60 serum and not by tbr serum. in subcellular fractions of rs ... | 1985 | 2981886 |
| a second nuclear protein is encoded by epstein-barr virus in latent infection. | a region of the epstein-barr virus (ebv) genome that is important in inducing cell proliferation includes a single long open reading frame. part of this open reading frame has been fused to the lacz gene and expressed in escherichia coli. antisera to the fusion protein identify a protein in the nuclei of latently infected growth-transformed lymphocytes and in burkitt tumor cells grown in vitro. this nuclear protein is encoded by a different virus-gene than that which encodes the previously descr ... | 1985 | 2983420 |
| relationship between bacteriophage t4 and t6 dna topoisomerases. t6 39-protein subunit is equivalent to the combined t4 39- and 60-protein subunits. | t6 dna topoisomerase has been purified from bacteriophage t6 infected escherichia coli. unlike the t4 dna topoisomerase which has three subunits, it consists of two subunits of molecular weights 75,000 and 51,000. they are the products of t6 genes 39 and 52, respectively. the purified t6 enzyme can stimulate in vitro t6 dna replication. it has an atp-dependent dna relaxation activity similar to the t4 enzyme. either atp or datp can be used in both reactions. using a "western blotting" and radioi ... | 1985 | 2991231 |
| application of the mini-mu-phage for target-sequence-specific insertional mutagenesis of the herpes simplex virus genome. | an earlier technique for insertional mutagenesis of large viral genomes involved the insertion of the thymidine kinase (tk) gene at a specific target site, cotransfection of the fragment carrying the insertion with the intact viral genome, and selection of the progeny for viral recombinants expressing the tk gene. the inserted tk gene could then be replaced by cotransfection of the recombinant dna with fragments carrying a foreign sequence or a deletion in the target sequence. to enable the prob ... | 1985 | 2991892 |
| immunoelectron microscopic localization of the restriction endonuclease ecori in escherichia coli bs 5. | purified restriction endonuclease ecori isolated from escherichia coli bs 5 was used for the production of enzyme-specific igg antibodies in rabbits. for enzyme localization experiments, paraformaldehyde-glutaraldehyde-fixed cells were embedded and polymerized by a low-temperature procedure using lowicryl k4m. the immuno electron microscopic protein a-gold technique and an immuno-gold method revealed that 70% of the enzyme-specific labeling were located in the cell envelope whereas 30% were foun ... | 1985 | 2992974 |
| cloning and characterization of the cdnas for human and rabbit interleukin-1 precursor. | dna sequence complementary to the mrna for rabbit interleukin-1 precursor (preil-1) has been cloned from the cdna library constructed using partially purified poly(a)+rna from induced rabbit alveolar macrophages by mrna hybridization-translation assay. by using this cdna as a probe, human il-1 cdna was isolated from the cdna library prepared using poly(a)+rna from induced hl-60 cells, a human monocyte-like cell line. the amino acid sequences of the human and rabbit preil-1 deduced from the cdna ... | 1985 | 2994016 |
| cloning of shiga-like toxin structural genes from a toxin converting phage of escherichia coli. | the genes controlling high-level production of shiga-like toxin (slt) in escherichia coli were cloned from the slt converting phage 933j. this phage was isolated from a strain of e. coli that caused a foodborne outbreak of hemorrhagic colitis. the genes that convert normal e. coli to organisms producing high levels of toxin were cloned into the plasmid pbr328 and expressed in e. coli hb101. dna restriction mapping, subcloning, examination of the cloned gene products by minicell analysis, neutral ... | 1985 | 2994228 |
| expression of polypeptide segments of the human complement component c3 in e. coli: genetic and immunological characterization of cdna clones specific for the alpha-chain of c3. | the third component of complement c3 and its fragments have a central role in a variety of host defense mechanisms. the identification of functionally relevant c3 domains is important because of the marked functional versatility of the c3 molecule. several human c3 cdna clones from a human liver cdna library were isolated and characterized. a bacterial expression vector system was used to express cdna clones that were identified by an immunological screening procedure. the c3 cdna clones produce ... | 1985 | 2995491 |
| cloning, sequencing, and chromosomal localization of human term placental alkaline phosphatase cdna. | a human term (third trimester) placental alkaline phosphatase (plap; ec 3.1.3.1) cdna was isolated from a human placental lambda gt11 cdna library. the expression library was screened by using rabbit antibodies against plap and oligonucleotide probes. dna sequence analysis of a positive clone with an insert of 2.7 kilobase pairs allowed us to predict the complete amino acid sequence of plap (530 residues), which coincided with the reported 42 n-terminal amino acid sequence of plap except at posi ... | 1985 | 3001717 |
| immunity to heat-labile enterotoxins of porcine and human escherichia coli strains achieved with synthetic cholera toxin peptides. | antiserum to a synthetic peptide, ctp3, consisting of residues 50 to 64 of the b subunit of cholera enterotoxin (ct), a sequence which is conserved in heat-labile enterotoxins from escherichia coli strains of human (h-lt) or porcine (p-lt) origin, cross-reacted with and neutralized all three enterotoxins. it also cross-reacted with genetically engineered chimeric b subunit proteins in which h-lt amino acids had been substituted for the corresponding residues in p-lt. antisera against another ct ... | 1986 | 3009330 |
| a second protease of foot-and-mouth disease virus. | foot-and-mouth disease virus (fmdv) genes are expressed as a polyprotein which is rapidly processed into the four primary cleavage products l, p1, p2, and p3. in secondary cleavage reactions, these are further processed into the mature proteins. the fmdv l protein is located at the n terminus of the polyprotein and is the first gene product released from the nascent polyprotein. for analysis of its biological function, the l gene was mutated by site-directed mutagenesis of cloned cdna. in vitro ... | 1986 | 3009894 |
| construction of genomic libraries of mycobacterial origin: identification of recombinants encoding mycobacterial-specific proteins. | a complete genomic library from mycobacterium vaccae (2785 recombinants) and a partial genomic library of m. leprae and bcg (300 and 1750 clones, respectively) were constructed in the plasmid pbr322. bam hi was selected as the restriction endonuclease for obtaining dna cleavage products. evidence was obtained for limited expression of the cloned mycobacterial dna inserts in escherichia coli. a recombinant has been identified which codes for antigen immunoreactive with rabbit anti-m. leprae antib ... | 1986 | 3018107 |
| probable occurrence of brain basic protein factor i, an activator of phosphoprotein phosphatases. | basic protein factor i (bpfi was purified to homogeneity from bovine brain by boiling and trichloroacetic acid-precipitation of tissue homogenate, followed by deae-cellulose, sephadex g-150, affi-gel-phenothiazine, and bio-gel p-6dg chromatographic procedures. the preparation appeared as a single protein band in the sds-polyacrylamide gel electrophoresis with a minimal mr of 13,200. the factor was a basic protein as indicated by an estimated isoelectric point of ph 8.3 and a high content of amin ... | 1986 | 3019610 |