Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evidence for il-6 promoter nuclear activation in u937 cells stimulated with salmonella enterica serovar typhimurium porins. | interleukin-6 (il-6) is a pleiotropic cytokine and plays an active role in inflammatory and immune responses, contributing to a multitude of physiological and pathophysiological processes. in this study, we address the molecular mechanism of il-6 transcriptional induction and propose a correlation between activated nf-kappab localization and il-6 expression. in particular, we detected, by chip assay, that occupation of the il-6 gene promoter site is dependent on activated nf-kappab. in fact, aft ... | 2009 | 19825524 |
| simultaneous assay of every salmonella typhi gene using one million transposon mutants. | very high-throughput sequencing technologies need to be matched by high-throughput functional studies if we are to make full use of the current explosion in genome sequences. we have generated a very large bacterial mutant pool, consisting of an estimated 1.1 million transposon mutants and we have used genomic dna from this mutant pool, and illumina nucleotide sequencing to prime from the transposon and sequence into the adjacent target dna. with this method, which we have called tradis (transpo ... | 2009 | 19826075 |
| a novel inhibitor of chlamydophila pneumoniae protein kinase d (pknd) inhibits phosphorylation of cdsd and suppresses bacterial replication. | we have shown previously that chlamydophila pneumoniae contains a dual-specific ser/thr protein kinase that phosphorylates cdsd, a structural component of the type iii secretion apparatus. to further study the role of pknd in growth and development we sought to identify a pknd inhibitor to determine whether pknd activity is required for replication. | 2009 | 19828035 |
| salmonella enterica serovar israel causing perforating appendicitis. | 2010 | 19828346 | |
| [serovars of salmonella enterica subspecies enterica and its antimicrobial resistance in slaughterhouse pigs]. | a study was carried out in order to determine the prevalence of salmonella and its serovars among porcine slaughterhouses, to evaluate the antimicrobial resistance profiles and to know the presence of class 1 integrons as possible reservoir of resistance. from a total of 386 samples from four porcine slaughterhouses of buenos aires and santa fe provinces (argentina), 93 (24.1%) salmonella enterica subspecies enterica strains were identified, 52 (55.9%) from cecal contents and 41 (44.1%) from ile ... | 2009 | 19831314 |
| simultaneous enrichment of shiga toxin-producing escherichia coli o157 and o26 and salmonella in food samples using universal preenrichment broth. | universal preenrichment broth (upb) was compared with modified escherichia coli broth with novobiocin (mec+n) for enrichment of shiga toxin-producing e. coli o157 and o26, and with buffered peptone water (bpw) for preenrichment of salmonella enterica. ten strains each of the three pathogens were inoculated into beef and radish sprouts following thermal, freezing, or no treatment. with regard to o157 and o26, upb incubated at 42 degrees c recovered significantly more cells from inoculated beef th ... | 2009 | 19833028 |
| isolation of salmonella enterica in laying-hen flocks and assessment of eggshell contamination in france. | the present investigation was conducted in conjunction with the european union baseline study for the estimation of salmonella prevalence in laying-hen flocks. it aimed at evaluating eggshell contamination in farms positive for salmonella, characterizing the genetic patterns of salmonella strains and identifying the factors associated with salmonella contamination of eggshells. for this purpose, a total of 4,200 eggs were collected from 28 positive flocks and analyzed according to draft annex d ... | 2009 | 19833029 |
| a rapid screen of broth enrichments for salmonella enterica serovars enteritidis, hadar, heidelberg, and typhimurium by using an allelotyping multiplex pcr that targets o- and h-antigen alleles. | salmonella continues to cause significant foodborne outbreaks, best illustrated with recent outbreaks associated with peanut butter, raw tomatoes, and serrano peppers. to ascertain the likely source of the outbreak, bacterial typing is essential to this process. while pcr has become an important detection tool for pathogens in foods, pcr can also identify strain differences by targeting gene(s) or sequences exhibiting polymorphisms or variability in its distribution within the bacterial populati ... | 2009 | 19833046 |
| changes of physiological and biochemical properties of salmonella enterica serovar typhimurium by deletion of cpxr and lon genes using allelic exchange method. | to construct a novel salmonella attenuated live vaccine, the cpxr and lon genes were deleted from a wild-type salmonella enterica serovar typhimurium (s. typhimurium) using allelic exchange method, resulting in s. typhimurium ck31 (deltacpxr), ck38 (deltalon), and ck111 (deltacpxr/lon). these mutated strains were grown normally, as was the wild-type strain. the biochemical properties of the mutants remained highly similar to those of the wild-type. in comparison with the wild-type, 1.5 to 3.3-fo ... | 2009 | 19833153 |
| development of an oligonucleotide-based microarray to detect multiple foodborne pathogens. | escherichia coli o157:h7, salmonella enterica, listeria monocytogenes and campylobacter jejuni are considered important pathogens causing the most food-related human illnesses worldwide. current methods for pathogen detection have limitations in the effectiveness of identifying multiple foodborne pathogens. in this study, a pathogen detection microarray was developed using various 70-mer oligonucleotides specifically targeting the above pathogens. to reduce the cost of detection, each microarray ... | 2010 | 19833198 |
| myo-inositol transport by salmonella enterica serovar typhimurium. | in salmonella enterica serovar typhimurium, the genomic island gei4417/4436 has recently been identified to be responsible for myo-inositol (mi) utilization. here, two of the four island-encoded permeases are identified as the mi transporters of this pathogen. in-frame deletion of iolt1 (stm4418) led to a severe growth defect, and deletion of iolt1 (stm4419) to a slight growth defect in the presence of mi. these phenotypes could be complemented by providing the putative transporter genes in tran ... | 2010 | 19833776 |
| effect of x-ray treatments on inoculated escherichia coli o157: h7, salmonella enterica, shigella flexneri and vibrio parahaemolyticus in ready-to-eat shrimp. | this study was conducted to evaluate the inactivation effect of x-ray treatments on escherichia coli o157: h7, salmonella enteric (s. enterica), shigella flexneri (s. flexneri) and vibrio parahaemolyticus (v. parahaemolyticus) artificially inoculated in ready-to-eat (rte) shrimp. a mixed culture of three strains of each tested pathogen was used to inoculate rte shrimp. the shrimp samples were inoculated individually with selected pathogenic bacteria then aseptically placed in sterile plastic cup ... | 2009 | 19835772 |
| comparative analysis of acid resistance in listeria monocytogenes and salmonella enterica strains before and after exposure to poultry decontaminants. role of the glutamate decarboxylase (gad) system. | data on the ability of chemical poultry decontaminants to induce an acid stress response in pathogenic bacteria are lacking. this study was undertaken in order to compare the survival rates in acid broths of listeria monocytogenes and salmonella enterica strains, both exposed to and not exposed to decontaminants. the contribution of the glutamate decarboxylase (gad) acid resistance system to the survival of bacteria in acid media was also examined. four strains (l. monocytogenes serovar 1/2, l. ... | 2009 | 19835779 |
| rpos- and crp-dependent transcriptional control of salmonella typhi taia and hlye genes: role of environmental conditions. | a novel pathogenicity island, spi-18, carries the taia-hlye operon, encoding virulence factors in salmonella typhi. to determine the effects of certain environmental conditions on the expression of these genes, beta-galactosidase assays, rt-pcr reactions, western blot analyses and measurement of hemolytic activity were performed. the conditions studied are those likely found by s. typhi during infection in the human host. we found rpos-dependent transcriptional upregulation in low ph and high os ... | 2009 | 19835951 |
| supplement 2003-2007 (no. 47) to the white-kauffmann-le minor scheme. | this supplement reports the characterization of 70 new salmonella serovars recognized between 2003 and 2007 by the who collaborating center for reference and research on salmonella: 44 were assigned to salmonella enterica subspecies enterica, 11 to subspecies salamae, 5 to subspecies arizonae, 8 to subspecies diarizonae, one to subspecies houtenae and one to salmonella bongori. one new serovar, mygdal, displayed a new h factor, h:z(91). | 2010 | 19840847 |
| multistate outbreak of salmonella infections associated with small turtle exposure, 2007-2008. | turtle-associated salmonellosis was increasingly recognized in the united states during the 1960s, leading to a federal ban in 1975 on the sale of turtles <4 inches in carapace length (small turtles). although sporadic reports of turtle-associated salmonella are frequent, outbreaks are rare. in september 2007, several patients with salmonella enterica serotype paratyphi b var java infections reported recent turtle exposure. we conducted an investigation to determine the source and extent of the ... | 2009 | 19841114 |
| ndp52: the missing link between ubiquitinated bacteria and autophagy. | mammalian cells ubiquitinate bacteria that erroneously enter the cytosol and target these intruding microbes for destruction by autophagy. new work shows that the protein ndp52 directly binds to ubiquitinated bacteria and facilitates the assembly of an autophagic membrane that surrounds these invaders. | 2009 | 19841643 |
| the synergistic activity of triclosan and ciprofloxacin on biofilms of salmonella typhimurium. | triclosan is a biocide whose wide use has raised a debate about the potential benefits vs. hazards of the incorporation of antimicrobials in consumer products. the purpose of the present study was to determine whether exposure of biofilms of salmonella enterica serovar typhimurium to triclosan influences the tolerance of the bacteria towards antibiotics such as ciprofloxacin and vice versa. a synergistic antibiofilm activity was observed when the biofilms were treated with triclosan before or to ... | 2009 | 19843306 |
| identification of salmonella enterica subspecies i, salmonella enterica serovars typhimurium, enteritidis and typhi using multiplex pcr. | this study was designed to develop a multiplex pcr method with five specific primer pairs for the detection of salmonella spp., salmonella subspecies i, salmonella enterica serovars typhimurium, typhi and enteritidis. a multiplex pcr was constructed with five primer pairs for the detection of salmonella and pathogenic salmonella serovars, including a specific primer pair for salmonella typhi, based on the sequence comparison between genomic dna sequences of 12 salmonella strains. each primer pai ... | 2009 | 19843307 |
| induction of cxc chemokine messenger-rna expression in chicken oviduct epithelial cells by salmonella enterica serovar enteritidis via the type three secretion system-1. | the messenger-rna (mrna) expression of selected cytokines and chemokines in primary chicken oviduct epithelial cells (coec) was determined following in vitro infections with wild-type or type three secretion system (t3ss)-mutant salmonella enterica serovar enteritidis (se) strains. all se strains examined in this study elicited the expression of proinflammatory immune mediators including inducible nitric oxide synthase (inos), cxcli1 (k60), cxcli2 (il-8), ccli3 (k203), and ccli4 (mip-1beta). se ... | 2009 | 19848079 |
| diagnostic utility of a single widal test in the diagnosis of typhoid fever at aga khan university hospital (akuh), nairobi, kenya. | typhoid fever is a systemic illness caused by salmonella enterica serovar typhi (s. typhi) and is endemic in kenya, where a single widal test done on an acute phase serum is the most commonly used test. using a cut-off value of 1:320 for both o and h agglutinins as being diagnostic of s. typhi, only 18 (26%) of our patients had diagnostic titres; 37 (53.6%) had o and h titres less than 1:40. our study showed that widal testing done on an acute phase serum of a patient suspected to have typhoid f ... | 2010 | 19850607 |
| identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks. | in this study, a novel avian beta-defensin (avbd) was isolated from duck pancreas. the complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. homology, characterization and comparison of the gene with avbd from other avian species confirmed that it was duck avbd2. the mrna expression of the gene was analyzed in 17 tissues from 21-day-old ducks. avbd2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed ... | 2009 | 19851734 |
| effect of adjuvants on immune response and protective immunity elicited by recombinant hsp60 (groel) of salmonella typhi against s. typhi infection. | heat shock proteins (hsps) have been reported to be dominant antigens for the host immune response to various pathogens and thus, have great potential for use in vaccination. in the present study, we evaluated the immunogenicity and protective efficacy of groel of salmonella enterica serovar typhi against lethal infection by s. typhi ty2 in mice with or without adjuvants. anti groel-igg titers were significantly higher in mice immunized with either groel-alone or in combination with alum/complet ... | 2010 | 19851830 |
| phage-type specific markers identified by diversity arrays technology (dart) analysis of salmonella enterica ssp. enterica serovars enteritidis and typhimurium. | diversity arrays technology (dart) was applied to differentiate between s. enterica serovar enteritidis and typhimurium strains, respectively. ten and eleven, mainly phage and plasmid-related markers were identified for serovars enteritidis and typhimurium. in combination, these markers can be used for subtyping among and within phage types. | 2010 | 19852988 |
| evaluation of live-attenuated salmonella vaccines expressing campylobacter antigens for control of c. jejuni in poultry. | campylobacter jejuni is a zoonotic bacterial pathogen of worldwide importance. it is estimated that 460,000 human infections occur in the united kingdom per annum and these involve acute enteritis and may be complicated by severe systemic sequelae. such infections are frequently associated with the consumption of contaminated poultry meat and strategies to control c. jejuni in poultry are expected to limit pathogen entry into the food chain and the incidence of human disease. toward this aim, a ... | 2010 | 19853682 |
| beta-lactam resistance in salmonella strains isolated from retail meats in the united states by the national antimicrobial resistance monitoring system between 2002 and 2006. | ampicillin-resistant (amp(r)) salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(cmy), bla(tem), bla(shv), bla(oxa), and bla(ctx-m)) and class 1 integrons. among the amp(r) isolates, 66.9% were resistant to five or more antimicrobials and 4.9% were resistant to 10 or more antimicrobials. coresistance to ... | 2009 | 19854922 |
| phage therapy to reduce preprocessing salmonella infections in market-weight swine. | contamination of meat products with food-borne pathogens usually results from the carcass coming in contact with the feces of an infected animal during processing. in the case of salmonella, pigs can become colonized with the organism during transport and lairage from contaminated trailers and holding pens, resulting in increased pathogen shedding just prior to processing. increased shedding, in turn, amplifies the likelihood of carcass contamination by magnifying the amount of bacteria that ent ... | 2010 | 19854929 |
| infected abdominal aortic aneurysm caused by nontyphoid salmonella in an immunocompromised patient with rheumatoid arthritis. | nontyphoid salmonella strains are important pathogens commonly found worldwide, typically causing gastrointestinal illness. here, we report a case of a 66-yearold man with an abdominal aortic infected (or so-called mycotic) aneurysm caused by salmonella enterica subsp. enterica serovar enteritidis (s. enteritidis). he had multiple risk factors for atherosclerosis: age over 60, a long history of smoking, an 8-year history of diabetes mellitus, and a 10-year history of rheumatoid arthritis treated ... | 2009 | 19856069 |
| molecular epidemiology and genetic environment of acquired bla acc-1 in salmonella enterica serotype livingstone causing a large nosocomial outbreak in tunisia. | eighty-four isolates of salmonella enterica serovar livingstone were collected from patients hospitalized in a pediatric ward in sfax hospital (south tunisia). these isolates were responsible for two nosocomial outbreaks in 2000 and 2002. twenty-eight clinical isolates of s. enterica serovar livingstone were also obtained in two other tunisian hospitals in monastir (central tunisia) and tunis (north tunisia), respectively, in 2002 and 2003. pulsed-field gel electrophoresis yielded that these iso ... | 2009 | 19857134 |
| ctx-m-2-producing salmonella typhimurium isolated from pediatric patients and poultry in brazil. | ten salmonella enterica serovar typhimurium isolates producing ctx-m-2 extended-spectrum beta-lactamase were identified from clinical and poultry sources in two distant cities in brazil between 2003 and 2004. they included two isolates from pediatric patients and eight isolates from poultry or its environment. all isolates exhibited coresistance to non-beta-lactam antimicrobials including tetracycline and trimethoprim/sulfamethoxazole. the ctx-m-2 gene was located on transferable plasmids with s ... | 2009 | 19857139 |
| systematic analysis of the ssrab virulon of salmonella enterica. | intracellular salmonella enterica serovar typhimurium deploys the salmonella pathogenicity island 2 (spi2)-encoded type iii secretion system (t3ss) to modify host cell functions and accomplish intracellular replication. this virulence function is controlled by the two-component system ssrab that regulates the expression of several operons in spi2 and, in addition, a large number of genes for non-spi2-encoded effector proteins. here, we analyzed the relative expression levels of members of the ss ... | 2010 | 19858298 |
| division of the salmonella-containing vacuole and depletion of acidic lysosomes in salmonella-infected host cells are novel strategies of salmonella enterica to avoid lysosomes. | salmonella has evolved several strategies to counteract intracellular microbicidal agents like reactive oxygen and nitrogen species. however, it is not yet clear how salmonella escapes lysosomal degradation. some studies have demonstrated that salmonella can inhibit phagolysosomal fusion, whereas other reports have shown that the salmonella-containing vacuole (scv) fuses/interacts with lysosomes. here, we have addressed this issue from a different perspective by investigating if the infected hos ... | 2010 | 19858305 |
| ceftazidime-resistant salmonella enterica, morocco. | 2009 | 19861080 | |
| psgi15, a small cole-like qnrb19 plasmid of a salmonella enterica serovar typhimurium strain carrying salmonella genomic island 1 (sgi1). | 2010 | 19861336 | |
| genotyping, plasmid analysis, and antimicrobial susceptibility of salmonella enterica serotype enteritidis isolates from humans and chickens in central taiwan. | salmonella enterica serotype enteritidis (se) is the most frequent etiological agent of human salmonellosis. the molecular epidemiology and antimicrobial susceptibility of human and chicken isolates of se were examined. | 2009 | 19864196 |
| a group of paratyphoid bacilli from animals closely resembling those found in man. | 1. in addition to the paratyphoid bacilli already named there exists a group which occurs in a variety of animals and which culturally is the same as bacillus schottmülleri. as a rule this group can be separated from the latter by the type of clumps formed when bouillon cultures are used as antigens, while other antigens and complement fixation tests have failed to differentiate it. agglutination absorption tests sharply separate the animal from the human paratyphoids. 2. no differences have bee ... | 1920 | 19868428 |
| bacilli of the hog-cholera group (bacillus cholerae suis) in man. | 1. the organisms isolated by hirschfeld from febrile cases resembling paratyphoid fever and named paratyphoid c can be placed in the hog-cholera bacillus group by their agglutination absorption properties though they are not typical culturally. 2. when fed to a pig a febrile disease resulted from which the animal recovered. after injection of hog-cholera virus the organisms fed were found generally, distributed and some of them had lost cultural characters so that they are brought into the class ... | 1920 | 19868429 |
| the use of chromogenic bacteria as coloured substitutes for pathogens: a simple strategy during design and development of a new method for sample pretreatment. | in the present study, chromogenic (red) bacteria were used to simulate actual target bacteria during set-up and optimization of an isolation process of bacteria, designed for food samples. isolation of bacteria from food in the context of molecular biological detection of food pathogens is a multistep process. development of such a separation method requires continuous monitoring of the location of the presumable targets in the sample tubes. therefore, red-coloured pigmented bacteria were used a ... | 2010 | 19874486 |
| molecular basis and evolution of multiple drug resistance in the foodborne pathogen salmonella enterica serovar ohio. | the molecular basis and evolution of multidrug resistance were established for 54 isolates of salmonella enterica serovar ohio, recorded between 1991 and 2005 in asturias, a northern region of spain. all isolates were closely related, as shown by cluster analysis of xbai-blni combined profiles. of these, 33.3% were resistant to one or more unrelated agent(s). sulphonamides, streptomycin, tetracycline, and trimethoprim, encoded by sul1, aada1, tet(a) or tet(b), and dfra1, respectively, were the m ... | 2010 | 19877766 |
| development of a live oral attaching and effacing escherichia coli vaccine candidate using vibrio cholerae cvd 103-hgr as antigen vector. | attaching and effacing escherichia coli (aeec) share the ability to induce pedestal formation and intimate adherence of the bacteria to the intestinal epithelial cell and effacement of microvilli of epithelial tissue. the locus of enterocyte effacement (lee) pathogenicity island encodes the ability to induce attaching and effacing (a/e) lesions and contains the gene eae, which encodes intimin, an outer membrane protein that is an adhesin for a/e lesion formation. here we show the utility of usin ... | 2010 | 19878715 |
| functional analysis of the nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (cobt) enzyme, involved in the late steps of coenzyme b12 biosynthesis in salmonella enterica. | in salmonella enterica, the cobt enzyme activates the lower ligand base during the assembly of the nucleotide loop of adenosylcobalamin (adocbl) and other cobamides. previously, mutational analysis identified a class of alleles (class m) that failed to restore adocbl biosynthesis during intragenic complementation studies. to learn why class m cobt mutations were deleterious, we determined the nature of three class m cobt alleles and performed in vivo and in vitro functional analyses guided by av ... | 2010 | 19880598 |
| sdia bridges chemical signaling between salmonella enterica serovar typhimurium and yersinia enterocolitica in mice. | 2010 | 19880607 | |
| multilocus variable-number tandem-repeat analysis and plasmid profiling to study the occurrence of blacmy-2 within a pulsed-field gel electrophoresis-defined clade of salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium circulating in food animal populations and carrying resistance to antimicrobial agents represents a human health risk. recently, a new clade of s. typhimurium, wa-typ035/187, was reported in cattle and humans in the pacific northwest, united states of america. the objective of this study was to describe a possible mechanism of acquisition of expanded-spectrum cephalosporin resistance in this clade. ceftazidime resistance increased steadily among wa-typ035/ ... | 2010 | 19880639 |
| salmonella enterica serovar typhimurium trxa mutants are protective against virulent challenge and induce less inflammation than the live-attenuated vaccine strain sl3261. | in salmonella enterica serovar typhimurium, trxa encodes thioredoxin 1, a small, soluble protein with disulfide reductase activity, which catalyzes thiol disulfide redox reactions in a variety of substrate proteins. thioredoxins are involved as antioxidants in defense against oxidative stresses, such as exposure to hydrogen peroxide and hydroxyl radicals. we have made a defined, complete deletion of trxa in the mouse-virulent s. typhimurium strain sl1344 (sl1344 trxa), replacing the gene with a ... | 2010 | 19884329 |
| norepinephrine augments salmonella enterica-induced enteritis in a manner associated with increased net replication but independent of the putative adrenergic sensor kinases qsec and qsee. | stress has long been correlated with susceptibility to microbial infection. one explanation for this phenomenon is the ability of pathogens to sense and respond to host stress-related catecholamines, such as norepinephrine (ne). in gram-negative enteric pathogens, it has been proposed that ne may facilitate growth by mediating iron supply, or it may alter gene expression by activating adrenergic sensor kinases. the aim of this work was to investigate the relative importance of these processes in ... | 2010 | 19884332 |
| a new generation of stable, nonantibiotic, low-copy-number plasmids improves immune responses to foreign antigens in salmonella enterica serovar typhi live vectors. | we hypothesized that adequately engineered attenuated salmonella enterica serovar typhi strains can serve as multivalent mucosal live vector vaccines to immunize against unrelated human pathogens. toward this ultimate goal, we have developed a novel genetic stabilization system for antigen-expressing plasmids, engineered to encode the single-stranded binding protein (ssb), an essential protein involved in dna metabolism which was deleted from the live vector chromosome. we utilized full-length p ... | 2010 | 19884333 |
| unusual class 1 integron configuration found in salmonella genomic island 2 from salmonella enterica serovar emek. | salmonella genomic island 2 (sgi2) is an independently derived genomic island related to sgi1 with the integron in a different position. the integron in sgi2 was found to include an additional 2.1 kb derived from the tni module of tn5058, tn502, or tn512 that was not detected previously. independent evolution of the backbone was confirmed with 21 single base differences found in over 11.5 kb, representing 40% of the 27.4-kb sgi2 backbone. | 2010 | 19884375 |
| first report of extended-spectrum-beta-lactamase-producing salmonella enterica serovar kentucky isolated from poultry in ireland. | therapy of invasive human salmonellosis is complicated by increasing antimicrobial resistance. food animals are the principal source of infection with nontyphoid salmonella. we report the emergence of broad-spectrum-cephalosporin resistance in salmonella enterica serovar kentucky in poultry in ireland. | 2010 | 19884382 |
| multi-immunogenic outer membrane vesicles derived from an msbb-deficient salmonella enterica serovar typhimurium mutant. | to develop low endotoxic and multi-immunogenic outer membrane vesicles (omvs), a deletion mutant of the msbb gene in salmonella enterica serovar typhimurium (s. typhimurium) was used as a source of low endotoxic omv, and an expression vector of the canine parvovirus (cpv) vp2 epitope fused to the bacterial ompa protein was constructed and transformed into the salmonella deltaamsbb mutant. in a lethality test, balb/c mice injected intraperitoneally with the salmonella deltaamsbb mutant survived f ... | 2009 | 19884791 |
| antimicrobial resistance genes in salmonella enterica isolates from poultry and swine in thailand. | a total of 184 resistant salmonella enterica isolated from poultry and swine were investigated for the distribution of and associations between various antimicrobial resistance genes. all the isolates were screened for the presence of class 2 and 3 integrase genes and 18 resistance genes corresponding to their resistance phenotypes. none of the isolates carried class 2 and 3 integrons. the investigated resistance genes were responsible for resistance in 78% of the isolates. all the strains harbo ... | 2009 | 19887742 |
| inhibition of fungi and gram-negative bacteria by bacteriocin bactn635 produced by lactobacillus plantarum sp. tn635. | the aim of this study was to evaluate 54 lactic acid bacteria (lab) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. the strain designed tn635 has been selected for advanced studies. the supernatant culture of this strain inhibits the growth of all tested pathogenic including the four gram-negative bacteria (salmonella enterica atcc43972, pseudomonas aeruginosa atcc 49189, hafnia sp. and ... | 2010 | 19888697 |
| detection of mutations in the gyra gene in fluoroquinolone resistance salmonella enterica serotypes typhi and paratyphi a isolated from the infectious diseases hospital, kuwait. | enteric fever due to salmonella enterica is a major health problem, and fluoroquinolones such as ciprofloxacin are mostly the antibiotic of choice for treatment. resistance to ciprofloxacin has been noticed to increase due to the emergence of new mutations in the bacterial dna. | 2010 | 19889623 |
| physical and chemical characterization and immunologic properties of salmonella enterica serovar typhi capsular polysaccharide-diphtheria toxoid conjugates. | typhoid fever remains a serious public health problem in developing countries, especially among young children. recent studies showed more than 50% of typhoid cases are in children under 5 years old. licensed vaccines, such as salmonella enterica serovar typhi capsular vi, did not confer protection against typhoid fever for this age group. vi conjugate, prepared by binding vi to pseudomonas aeruginosa recombinant exoprotein a (repa), induces protective levels of antibody at as young as 2 years o ... | 2010 | 19889941 |
| listeria monocytogenes does not survive ingestion by acanthamoeba polyphaga. | listeria monocytogenes is a ubiquitous bacterium capable of infecting humans, particularly pregnant women and immunocompromised individuals. although the intracellular invasion and pathogenesis of listeriosis in mammalian tissues has been well studied, little is known about the ecology of l. monocytogenes , and in particular the environmental reservoir for this bacterium has not been identified. this study used short-term co-culture at 15, 22 and 37 degrees c to examine the interaction of l. mon ... | 2010 | 19892759 |
| case report of pediatric gastroenteritis due to ctx-m-15 extended-spectrum beta-lactamase-producing salmonella enterica serotype enteritidis. | a clinical isolate of salmonella enterica serotype enteritidis in korea was found to produce the extended-spectrum beta-lactamase ctx-m-15. the isolate was recovered in 2008 from the stool of a 3-yr-old boy with gastroenteritis. this isolate was found to be resistant to multiple drugs, including ampicillin, piperacillin, cefotaxime, ceftazidime, cefepime, and aztreonam. the resistance to cefotaxime was transferred by conjugation to recipient escherichia coli j53. the patient was eventually succe ... | 2009 | 19893356 |
| antimicrobial substances produced by coliform strains active against foodborne pathogens. | in the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in rio de janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to nine different antibiotics. two strains (6.4%) exhibited inhibitory activity against the indicator strains, escherichia coli lmifrj and salmonella ente ... | 2010 | 19895262 |
| effects of allspice, cinnamon, and clove bud essential oils in edible apple films on physical properties and antimicrobial activities. | essential oils (eos) derived from plants are rich sources of volatile terpenoids and phenolic compounds. such compounds have the potential to inactivate pathogenic bacteria on contact and in the vapor phase. edible films made from fruits or vegetables containing eos can be used commercially to protect food against contamination by pathogenic bacteria. eos from cinnamon, allspice, and clove bud plants are compatible with the sensory characteristics of apple-based edible films. these films could e ... | 2009 | 19895483 |
| antibacterial effects of allspice, garlic, and oregano essential oils in tomato films determined by overlay and vapor-phase methods. | physical properties as well as antimicrobial activities against escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes of allspice, garlic, and oregano essential oils (eos) in tomato puree film-forming solutions (tpffs) formulated into edible films at 0.5% to 3% (w/w) concentrations were investigated in this study. antimicrobial activities were determined by 2 independent methods: overlay of the film on top of the bacteria and vapor-phase diffusion of the antimicrobial from th ... | 2009 | 19895486 |
| tlr2-, tlr4- and myd88-independent acquired humoral and cellular immunity against salmonella enterica serovar typhimurium. | toll-like receptors (tlr) are central to pathogen recognition by the innate immune system. however, their role in the generation of acquired immunity is less clear. using experimental salmonella enterica serovar typhimurium (st) infection of mice, we determined the role of tlr2, tlr4 and of the tlr adaptor protein myeloid differentiation factor 88 (myd88) in the generation of specific t- and b-cell responses against this pathogen. when infected with an attenuated st strain, mice deficient in tlr ... | 2010 | 19895846 |
| salmonella infection of a total knee replacement. | salmonella enterica serotype choleraesuis is a rare infective pathogen in total knee arthroplasty and accounts for less than 1% of all cases. in the united kingdom, the most prevalent organisms are staphylococci, which account for over 50% of cases. we present an interesting case report of an infected revision total knee replacement following foreign travel to a region with an increased prevalence of salmonella. the patient presented typically with an effused knee and raised inflammatory markers ... | 2010 | 19897369 |
| short-term signatures of evolutionary change in the salmonella enterica serovar typhimurium 14028 genome. | salmonella enterica serovar typhimurium is a gram-negative pathogen that causes gastroenteritis in humans and a typhoid-like disease in mice and is often used as a model for the disease promoted by the human-adapted s. enterica serovar typhi. despite its health importance, the only s. typhimurium strain for which the complete genomic sequence has been determined is the avirulent lt2 strain, which is extensively used in genetic and physiologic studies. here, we report the complete genomic sequenc ... | 2010 | 19897643 |
| bistable expression of csgd in biofilm development of salmonella enterica serovar typhimurium. | bacterial persistence in the environment and in the infected host is often aided by the formation of exopolymer-enclosed communities known as biofilms. heterogeneous gene expression takes place in microcompartments formed within the complex biofilm structure. this study describes cell differentiation within an isogenic bacterial cell population based on the example of biofilm formation by salmonella enterica serovar typhimurium. we analyzed the expression of the major biofilm regulator csgd at t ... | 2010 | 19897646 |
| promoter and riboswitch control of the mg2+ transporter mgta from salmonella enterica. | the mgta protein from salmonella enterica serovar typhimurium mediates mg(2+) uptake from the periplasm into the cytoplasm. here we report that the phop/phoq two-component regulatory system, which responds to periplasmic mg(2+), governs mgta transcription initiation at all investigated mg(2+) concentrations and that the mg(2+)-sensing 5' leader region of the mgta gene controls transcription elongation into the mgta coding region when salmonella is grown in media with <50 mum mg(2+). overexpressi ... | 2010 | 19897653 |
| cells in shearable and nonshearable regions of salmonella enterica serovar enteritidis biofilms are morphologically and physiologically distinct. | cellular morphology, exopolymer chemistry, and protein expression of shearable and nonshearable fractions of salmonella enterica serovar enteritidis biofilms were examined. biofilms were grown at a laminar flow velocity of 0.07 cm.s-1 for ~120 h, resulting in biofilms with a thickness (mean +/- sd) of 43 +/- 24 microm. an empirically determined shear-inducing flow (1.33 cm.s-1) was then applied for 5 min, effectively reducing biofilm thickness by ~70% and leaving 13 +/- 6 microm of nonshearable ... | 2009 | 19898535 |
| epidemic multiple drug resistant salmonella typhimurium causing invasive disease in sub-saharan africa have a distinct genotype. | whereas most nontyphoidal salmonella (nts) are associated with gastroenteritis, there has been a dramatic increase in reports of nts-associated invasive disease in sub-saharan africa. salmonella enterica serovar typhimurium isolates are responsible for a significant proportion of the reported invasive nts in this region. multilocus sequence analysis of invasive s. typhimurium from malawi and kenya identified a dominant type, designated st313, which currently is rarely reported outside of africa. ... | 2009 | 19901036 |
| the salmonella enterica serotype typhi vi capsular antigen is expressed after the bacterium enters the ileal mucosa. | salmonella enterica serotype typhi, the etiological agent of typhoid fever, produces the vi capsular antigen, a virulence factor absent in salmonella enterica serotype typhimurium. previous studies suggest that the capsule-encoding viab locus reduces inflammatory responses in intestinal tissue; however, there are currently no data regarding the in vivo expression of this locus. here we implemented direct and indirect methods to localize and detect vi antigen expression within polarized intestina ... | 2010 | 19901065 |
| transfer of plasmid-mediated ctx-m-9 from salmonella enterica serotype virchow to enterobacteriaceae in human flora-associated rats treated with cefixime. | food animals are a potential source of ctx-m resistance genes for humans. we evaluated the transfer of the bla(ctx-m-9) gene from an animal strain of salmonella enterica serotype virchow to enterobacteriaceae of the human intestinal flora by using human flora-associated (hfa) rats with and without cefixime treatment. in the absence of antibiotic, no transconjugant enterobacteria were found in the feces of hfa rats. however, the transfer rate was high if escherichia coli j5 recipient strains were ... | 2010 | 19901088 |
| sensitive and rapid molecular detection assays for salmonella enterica serovars typhimurium and heidelberg. | salmonella enterica is a significant cause of gastroenteritis worldwide, with serovars typhimurium and heidelberg being particularly prevalent, which have broad host ranges infecting poultry, dairy animals, and humans. traditional methods used for the detection of salmonella from contaminated food products are time-consuming and labor-intensive. the aim of this study was to develop a sensitive and rapid pcr-based detection method with optimized specificity for high-throughput screening of food a ... | 2009 | 19903399 |
| uptake through glycoprotein 2 of fimh(+) bacteria by m cells initiates mucosal immune response. | the mucosal immune system forms the largest part of the entire immune system, containing about three-quarters of all lymphocytes and producing grams of secretory iga daily to protect the mucosal surface from pathogens. to evoke the mucosal immune response, antigens on the mucosal surface must be transported across the epithelial barrier into organized lymphoid structures such as peyer's patches. this function, called antigen transcytosis, is mediated by specialized epithelial m cells. the molecu ... | 2009 | 19907495 |
| consumption of fresh fruit juice: how a healthy food practice caused a national outbreak of salmonella panama gastroenteritis. | in spring 2008, 15 salmonella panama laboratory-confirmed cases were reported within 2 weeks, twice the average annual number of reported cases of this infrequent serotype in the netherlands. to identify the source responsible for this national outbreak, we carried out an epidemiological, microbiological, and trace-back investigation. in total, 33 cases were reported, and a matched case-control study (23 cases/24 controls) identified consumption of fresh (unpasteurized) fruit juice purchased fro ... | 2010 | 19909088 |
| transcriptome analysis of genes controlled by luxs/autoinducer-2 in salmonella enterica serovar typhimurium. | the enteric pathogen salmonella enterica serovar typhimurium uses autoinducer-2 (ai-2) as a signaling molecule. ai-2 requires the luxs gene for its synthesis. the regulation of global gene expression in salmonella typhimurium by luxs/ai-2 is currently not known; therefore, the focus of this study was to elucidate the global gene expression patterns in salmonella typhimurium as regulated by luxs/ai-2. the genes controlled by luxs/ai-2 were identified using microarrays with rna samples from wild-t ... | 2010 | 19909098 |
| identification of in vivo induced protein antigens of salmonella enterica serovar typhi during human infection. | during infectious disease episodes, pathogens express distinct subsets of virulence factors which allow them to adapt to different environments. hence, genes that are expressed or upregulated in vivo are implicated in pathogenesis. we used in vivo induced antigen technology (iviat) to identify antigens which are expressed during infection with salmonella enterica serovar typhi. we identified 7 in vivo induced (ivi) antigens, which included bcfd (a fimbrial structural subunit), grxc (a glutaredox ... | 2009 | 19911130 |
| real-time reverse-transcriptase--polymerase chain reaction for salmonella enterica detection from jalapeño and serrano peppers. | outbreaks of salmonella linked to fresh produce emphasize the need for rapid detection methods to curb the spread of foodborne pathogens. reverse-transcriptase-polymerase chain reaction (rt-pcr) detects the presence of mrna (shorter half-life than dna), with greater potential of detecting viable pathogens. real-time rt-pcr eliminates the need for gel electrophoresis and significantly enhances the speed of detection (<1 day) compared with traditional methods (>5 days). the objectives of this rese ... | 2010 | 19911882 |
| cluster of cases of salmonella enterica serotype rissen infection in a general hospital, italy, 2007. | in 2007, three strains of salmonella enterica serotype rissen (s. rissen) were isolated in the laboratory of diagnostic microbiology of the general hospital of prato, tuscany, italy, over a 1 month and half interval of time. the first isolate was recovered on january 26 from an outpatient with enteritis. then, two strains were isolated on february 16 and march 11 respectively, from central venous catheters of patients who were being hospitalized in two departments of the hospital. an epidemiolog ... | 2010 | 19912602 |
| genome wide evolutionary analyses reveal serotype specific patterns of positive selection in selected salmonella serotypes. | the bacterium salmonella enterica includes a diversity of serotypes that cause disease in humans and different animal species. some salmonella serotypes show a broad host range, some are host restricted and exclusively associated with one particular host, and some are associated with one particular host species, but able to cause disease in other host species and are thus considered "host adapted". five salmonella genome sequences, representing a broad host range serotype (typhimurium), two host ... | 2009 | 19912661 |
| inactivation of escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on spinach leaves by x-ray. | several recent foodborne disease outbreaks associated with leafy green vegetables, including spinach, have been reported. x-ray is a non-thermal technology that has shown promise for reducing pathogenic and spoilage bacteria on spinach leaves. inactivation of inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri on spinach leaves using x-ray at different doses (0.1, 0.2, 0.3, 0.5, 0.75, 1.0, 1.5 and 2.0 kgy) was studied. the effect of x-ray on col ... | 2010 | 19913687 |
| effects of x-ray radiation on escherichia coli o157:h7, listeria monocytogenes, salmonella enterica and shigella flexneri inoculated on shredded iceberg lettuce. | the main goal of this investigation was to study the efficacy of x-ray doses (0.1, 0.2, 0.3, 0.5, 0.75, 1.0, 1.5 and 2.0 kgy) on inoculated escherichia coli o157: h7, listeria monocytogenes, salmonella enterica and shigella flexneri on shredded iceberg lettuce. the second goal was to study the effect of x-ray on the inherent microflora counts and visual color of shredded iceberg lettuce during storage at 4 degrees c for 30 days. treatment with 1.0 kgy x-ray significantly reduced the population o ... | 2010 | 19913700 |
| epidemiological characteristics of salmonella enterica serovar typhimurium from healthy pigs in japan. | we characterized 53 salmonella enterica ser. typhimurium strains recovered from healthy pigs during 1998-1999 (n=12) and 2004-2005 (n=41) as to their carriage of dt104 spacer region, class 1 and 2 integrons, virulence genes (spvc, rck, and pefa), and xbai- and blni-pulsed-field gel electrophoresis (pfge) profiles. no dt104 strain was detected in 1998-1999, whereas 65.9% (27/41) of the strains in 2004-2005 were dt104 showing resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, t ... | 2010 | 19915327 |
| improving the aoac use-dilution method by establishing a minimum log density value for test microbes on inoculated carriers. | the aoac use-dilution methods, 955.14 (salmonella enterica), 955.15 (staphylococcus aureus), and 964.02 (pseudomonas aeruginosa), are used to measure the efficacy of disinfectants on hard inanimate surfaces. the methods do not provide procedures to assess log density of the test microbe on inoculated penicylinders (carrier counts). without a method to measure and monitor carrier counts, the associated efficacy data may not be reliable and repeatable. this report provides a standardized procedure ... | 2009 | 19916390 |
| development of a dna microarray to detect antimicrobial resistance genes identified in the national center for biotechnology information database. | to understand the mechanisms and epidemiology of antimicrobial resistance (ar), the genetic elements responsible must be identified. due to the myriad of possible genes, a high-density genotyping technique is needed for initial screening. to achieve this, ar genes in the national center for biotechnology information genbank database were identified by their annotations and compiled into a nonredundant list of 775 genes. a dna microarray was constructed of 70mer oligonucelotide probes designed to ... | 2010 | 19916789 |
| fitness costs and stability of a high-level ciprofloxacin resistance phenotype in salmonella enterica serotype enteritidis: reduced infectivity associated with decreased expression of salmonella pathogenicity island 1 genes. | the fitness costs associated with high-level fluoroquinolone resistance were examined for phenotypically and genotypically characterized ciprofloxacin-resistant salmonella enterica serotype enteritidis mutants (104-cip and 5408-cip; mic, >32 microg/ml). the stability of the fluoroquinolone resistance phenotype in both mutants was investigated to assess whether clones with better fitness could emerge in the absence of antibiotic selective pressure. mutants 104-cip and 5408-cip displayed altered m ... | 2010 | 19917752 |
| novel integron gene cassette arrays identified in a global collection of multi-drug resistant non-typhoidal salmonella enterica. | investigation of integron carriage in a global collection of multi-drug resistant salmonella enterica identified 3 unique class 1 integron gene cassette arrays not previously reported in this species. the present study used pcr and dna sequence analysis to characterize the structure of these gene cassette arrays. a approximately 4.0 kb integron containing the gene cassette array arr2/cmla5/bla (oxa10) /aada1 was found in isolates belonging to serovars isangi and typhimurium from south africa. a ... | 2010 | 19921331 |
| the phev phenylalanine trna gene klebsiella pneumoniae clinical isolates is an integration hotspot for possible niche-adaptation genomic islands. | horizontally acquired genomic islands may allow bacteria to conquer and colonize previously uncharted niches. four klebsiella pneumoniae trna gene insertion hotspots (arg6, asn34, met56, and phev) in 101 clinical isolates derived from blood, sputum, wound, bile or urine specimens were screened by long-range pcr for the presence or absence of integrated islands. the phev phenylalanine trna gene was the most frequently occupied site and harbored at least three entirely distinct types of islands: ( ... | 2010 | 19921332 |
| genomic and phenotypic variation in epidemic-spanning salmonella enterica serovar enteritidis isolates. | salmonella enterica serovar enteritidis (s. enteritidis) has caused major epidemics of gastrointestinal infection in many different countries. in this study we investigate genome divergence and pathogenic potential in s. enteritidis isolated before, during and after an epidemic in uruguay. | 2009 | 19922635 |
| adjuvant effects for oral immunization provided by recombinant lactobacillus casei secreting biologically active murine interleukin-1{beta}. | vaccine delivery systems using lactic acid bacteria are under development, but their efficiency is insufficient. autologous cytokines, such as interleukin-1beta (il-1beta), are potential adjuvants for mucosal vaccines and can be provided by recombinant lactic acid bacteria. the aim of this study was the construction and evaluation of recombinant lactobacillus casei producing il-1beta as an adjuvant delivery agent. the recombinant strain was constructed using an expression/secretion vector plasmi ... | 2010 | 19923575 |
| salmonella enterica serovar typhimurium mutants completely lacking the f(0)f(1) atpase are novel live attenuated vaccine strains. | the f(0)f(1) atpase plays a central role in both the generation of atp and the utilisation of atp for cellular processes such as rotation of bacterial flagella. we have deleted the entire operon encoding the f(0)f(1) atpase, as well as genes encoding individual f(0) or f(1) subunits, in salmonella enteric serovar typhimurium. these mutants were attenuated for virulence, as assessed by bacterial counts in the livers and spleens of intravenously infected mice. the attenuated in vivo growth of the ... | 2010 | 19925904 |
| binding of salmonella-specific antibody facilitates specific t cell responses via augmentation of bacterial uptake and induction of apoptosis in macrophages. | most antigens from intracellular bacteria or vaccines induce both humoral and cell-mediated immune responses, but interactions between these responses are not fully understood. this study aims to resolve how specific antibodies participate in the activation of specific t cells in protecting hosts against salmonella enterica serotype typhimurium (s. typhimurium) infection. | 2010 | 19929376 |
| bacteriophage therapy of salmonella enterica: a fresh appraisal of bacteriophage therapy. | the most serious criticisms leveled at bacteriophage therapy are as follows: phages induce neutralizing antibodies, phages are active only when administered shortly after bacterial infection, and phage-resistant bacteria emerge rapidly in the course of therapy. | 2010 | 19929381 |
| cellular cholesterol involvement in src, pkc, and p38/jnk transduction pathways by porins. | biological membranes are described as a mosaic of different domains where interactions between membrane components induce the formation of subdomains with different characteristics and functions. lipids play an important role in the formation of lipid-enriched microdomains where they dynamically associate to form platforms important for membrane protein sorting and construction of signaling complexes. cholesterol confined in lipid domains is a crucial component required by microorganisms, direct ... | 2009 | 19929574 |
| toll-like receptor 4 signalling through myd88 is essential to control salmonella enterica serovar typhimurium infection, but not for the initiation of bacterial clearance. | toll-like receptor-4 (tlr4) is important in protection against lethal salmonella enterica serovar typhimurium (s. typhimurium) infection. control of the early stages of sublethal s. typhimurium infection in mice depends on tlr4-dependent activation of macrophages and natural killer (nk) cells to drive an inflammatory response. tlr4 signals through the adapter proteins mal/myd88 and trif-related adaptor molecule (tram)/tir-domain-containing adaptor-inducing interferon-b (trif). in the mouse typho ... | 2009 | 19930040 |
| factors influencing survival of enterotoxigenic escherichia coli, salmonella enterica (serovar typhimurium) and vibrio parahaemolyticus in marine environments. | the presence and persistence of enterotoxigenic escherichia coli (etec) is poorly investigated in marine habitats. here we compared etec with the more studied fecal contaminant, salmonella enterica serotype typhimurium (s. enterica) and the marine bacteria vibrio parahaemolyticus. all three species of bacteria were culturable on agar plates during 8 weeks of incubation in seawater. however, the culturability of etec was positively affected by low temperature whereas v. parahaemolyticus was negat ... | 2010 | 19930458 |
| salmonella enterica serovar typhimurium lipopolysaccharide deacylation enhances its intracellular growth within macrophages. | modification of lipid a is essential for bacterial adaptation to its host. salmonella typhimurium lpxr potentially detoxifies lipid a by 3'-o-deacylation; however, the involvement of deacylation in its adaptation remains unclear. lpxr-dependent 3'-o-deacylation was observed in the stationary phase. when macrophages were infected with stationary phase bacteria, the intracellular growth of the lpxr-null strain was lower than that of the wild-type strain. furthermore, the expression level of induci ... | 2010 | 19932105 |
| cd8+ t cell adjuvant effects of salmonella flicd flagellin in live vaccine vectors or as purified protein. | salmonella flagellin, the flagellum structural subunit, has received particular interest as a vaccine adjuvant conferring enhanced immunogenity to soluble proteins or peptides, both for activation of antibody and cellular immune responses. in the present study, we evaluated the salmonella enterica flicd flagellin as a t cell vaccine adjuvant using as model the 9-mer (syvpsaeqi) synthetic h2(d)-restricted cd8(+) t cell-specific epitope (cs(280-288)) derived from the plasmodium yoelii circumsporoz ... | 2010 | 19932669 |
| conversion of glycerol to poly(3-hydroxypropionate) in recombinant escherichia coli. | we have developed the conversion of glycerol into thermoplastic poly(3-hydroxypropionate) [poly(3hp)]. for this, the genes for glycerol dehydratase (dhab1) of clostridium butyricum, propionaldehyde dehydrogenase (pdup) of salmonella enterica serovar typhimurium lt2, and polyhydroxyalkanoate (pha) synthase (phac1) of ralstonia eutropha were expressed in recombinant escherichia coli. poly(3hp) was accumulated up to 11.98% (wt/wt [cell dry weight]) in a two-step, fed-batch fermentation. the present ... | 2010 | 19933347 |
| genome-wide transposon mutagenesis identifies a role for host neuroendocrine stress hormones in regulating the expression of virulence genes in salmonella. | bacterial sensing of environmental signals plays a key role in regulating virulence and mediating bacterium-host interactions. the sensing of the neuroendocrine stress hormones epinephrine (adrenaline) and norepinephrine (noradrenaline) plays an important role in modulating bacterial virulence. we used mudj transposon mutagenesis to globally screen for genes regulated by neuroendocrine stress hormones in salmonella enterica serovar typhimurium. we identified eight hormone-regulated genes, includ ... | 2010 | 19933366 |
| prevalence of chlamydia trachomatis and neisseria gonorrhoeae in hiv-seropositive patients and gonococcal antimicrobial susceptibility: an update in thailand. | we surveyed the rate of chlamydial and gonococcal infections among human immunodeficiency virus (hiv)-seropositive patients in thailand as well as the current status of antimicrobial resistance of neisseria gonorrhoeae and determined the prevalence of penicillinase-producing n. gonorrhoeae (ppng) in thailand. a total of 1,158 endocervical swabs from 824 hiv-seropositive patients were collected to detect both organisms by gen-probe. the prevalences of chlamydial and gonococcal infection were 9.7 ... | 2009 | 19934542 |
| bovine muc1 inhibits binding of enteric bacteria to caco-2 cells. | inhibition of bacterial adhesion to intestinal epithelial receptors by the consumption of natural food components is an attractive strategy for the prevention of microbial related gastrointestinal illness. we hypothesised that muc1, a highly glycosylated mucin present in cows' milk, may be one such food component. purified bovine muc1 was tested for its ability to inhibit binding of common enteric bacterial pathogens to caco-2 cells grown in vitro. muc1 caused dose-dependent binding inhibition o ... | 2010 | 19936918 |
| efficacy of several vaccination programmes in commercial layer and broiler breeder hens against experimental challenge with salmonella enterica serovar enteritidis. | two experiments were performed to evaluate the protective effect of various vaccination combinations given at 5 and 9 weeks of age against experimental challenge with salmonella enterica serovar enteritidis (se) phage type 4 at 12 weeks of age. in experiment 1, groups of commercial layers were vaccinated by one of the following programmes: group 1, two doses of a se bacterin (layermune se); group 2, one dose of a live salmonella enterica serovar gallinarum vaccine (cevac sg9r) followed by one do ... | 2009 | 19937524 |
| a comparison of the efficacy of cleaning and disinfection methods in eliminating salmonella spp. from commercial egg laying houses. | effective terminal cleaning and disinfection (c&d) is regarded as a necessary step for the elimination of salmonella spp. from laying houses. a total of 60 commercial laying houses that had housed laying flocks infected with salmonella enterica serovar enteritidis or salmonella enterica serovar typhimurium that were representative of all production systems (cage, barn, free-range) were intensively sampled immediately after c&d as well as in the follow-on flock. the procedures investigated were: ... | 2009 | 19937529 |
| study of an unusual paratyphoid epornitic in canaries (serinus canaria). | high mortalities in 17 canary flocks from different regions of tehran, iran, were reported. necropsy and histopathologic examination revealed necrotic hepatitis and overall congestive septicaemia in carcasses. salmonella enterica was isolated from 34 examined samples, two samples from each flock, including visceral organs of carcasses and droppings of live diseased birds. all isolates were typed as salmonella enterica serovar typhimurium by conventional serotyping. antibiotic resistance profilin ... | 2009 | 19937532 |