Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| microbial load and antimicrobial property of two nigerian herbal remedies. | the use of herbal remedies had increased significantly in the last one decade in nigeria. this has led to the production of herbal products with bogus claims. the microbial quality and antibacterial properties of two nigerian herbal remedies with such claimed efficacy of curing all manners of microbial diseases were assessed. the herbal remedies were discovered to be contaminated with the following microorganisms: bacillus subtilis, bacillus coagulans, bacillus cereus, basidiobotrytis sp, oedoce ... | 2007 | 20162058 |
| evaluation of antimicrobial activity of the stem bark of cylicodiscus gabunensis (mimosaceae). | ethyl acetate (ea) extract of the stem bark of cylicodiscus gabunensis (cg) was analysed phytochemically and evaluated for its antimicrobial activity against 17 pathogenic species isolated from patient: escherichia coli, klebsiella pneumoniae, shigella dysenteriae, shigella flexneri, morganella morganii, proteus vulgaris, proteus mirabilis, salmonella typhi, citrobacter freundii, enterobacter cloacae, enterobacter agglomerans, staphylococcus aureus, streptococcus feacalis, pseudomonas aeruginosa ... | 2006 | 20162076 |
| synthesis, spectroscopic and biological studies on the new symmetric schiff base derived from 2,6-diformyl-4-methylphenol with n-aminopyrimidine. | a phenol based novel schiff base polydentate symmetric ligand was prepared. the complexes were prepared by reacting ligand and the metal chlorides of cu (ii), ni(ii), co(ii) and fe(ii) in methanol to get a series of mononuclear complexes. the complexes were characterized by elemental analyses, conductivity measurements, magnetic susceptibility data, ir, uv-vis, nmr and api-es mass spectral data. the mononuclear structure of the complexes was confirmed on the basis of elemental analyses, magnetic ... | 2010 | 20163896 |
| evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. | the aim of this study was to evaluate diffusion and dilution methods for determining the antibacterial activity of plant extracts and their mixtures. several methods for measurement of the minimal inhibitory concentration (mic) of a plant extract are available, but there is no standard procedure as there is for antibiotics. we tested different plant extracts, their mixtures and phenolic acids on selected gram-positive (staphylococcus aureus, bacillus cereus, and listeria monocytogenes) and gram- ... | 2010 | 20171250 |
| synthesis and antibacterial assessment of n-[4-(4-substituted phenyl)-1,3-thiazol-2-yl]-1,3,5-triazin-2-amine. | in a wide search program towards new and efficient antibacterial agents, we assessed the extent to which physicochemical properties can be exploited to promote antibacterial activity associated with a series of substituted s-triazine. the synthesized compounds (1a-12b) were subsequently screened for their in vitro antibacterial activity against three gram positive (bacillus subtilis, bacillus cereus, staphylococcus aureus) and three gram-negative microorganism (salmonella typhi, escherichia coli ... | 2009 | 20177467 |
| bacterial deoxyribonucleoside kinases are poor suicide genes in mammalian cells. | transfer of deoxyribonucleoside kinases (dnks) into cancer cells increases the activity of cytotoxic nucleoside analogues. it has been shown that bacterial dnks, when introduced into escherichia coli, sensitize this bacterium toward nucleoside analogues. we studied the possibility of using bacterial dnks, for example deoxyadenosine kinases (daks), to sensitize human cancer cells to gemcitabine. stable and transient transfections of bacterial dnks into human cells showed that these were much less ... | 2009 | 20183574 |
| a new prenylated flavanoid with antibacterial activity from propolis collected in egypt. | a novel prenylated flavanoid, isonymphaeol-d (1), together with two known compounds, isonymphaeol-b (2) and nymphaeol-b (3), were isolated from egyptian propolis. the structures of the isolated compounds were determined by various spectroscopic methods. 1 exhibited antibacterial activity against gram-positive (bacillus cereus, staphylococcus aureus) and gram-negative strains (serratia sp., pseudomonos sp., escherichia coli). | 2010 | 20184018 |
| identification and characterization of microorganisms from earthworm viscera for the conversion of fish wastes into liquid fertilizer. | five bacteria isolated from earthworm viscera and identified as brevibacillus agri, bacillus cereus, bacillus licheniformis, and brevibacillus parabrevis by 16s rrna sequencing were employed in the conversion of fish wastes generated from a restaurant specializing in sliced raw fish into fertilizer. within 120h after inoculation of autoclaved fish waste with 5.15 x 10(5) cfu ml(-1) mixed isolates, the amount of dry sludge decreased from 29.4 to 0.2g, the ph changed from 7.05 to 5.70, and the cel ... | 2010 | 20189380 |
| zwittermicin a: synthesis of analogs and structure-activity studies. | analogs and diastereomers of the natural product zwittermicin a were prepared. sar studies of these compounds reveal the antifungal activity to be dependent singularly upon the natural constitution and configuration. | 2010 | 20189808 |
| identification of bacillus cereus genes specifically expressed during growth at low temperatures. | the mechanisms involved in the ability of bacillus cereus to multiply at low temperatures were investigated. it was assumed that many genes involved in cold acclimation would be upregulated at low temperatures. recombinase-based in vivo expression technology (ivet) was adapted to the detection of the transient activation of b. cereus promoters during growth at 10 degrees c. four independent screenings of a promoter library from type strain atcc 14579 were performed, and 17 clones were isolated. ... | 2010 | 20190083 |
| rapid detoxification of cereulide in bacillus cereus food poisoning. | bacillus cereus is recognized as a major pathogenic bacterium that causes food poisoning and produces gastrointestinal diseases of 2 types: emetic and diarrheal. the emetic type, which is often linked to pasta and rice, arises from a preformed toxin, cereulide, in food. rapid and accurate diagnostic methods for this emetic toxin are important but are limited. here we describe 3 patients with b cereus food poisoning in which cereulide was detected and measured sequentially. three family members b ... | 2010 | 20194285 |
| using enzymes to remove biofilms of bacterial isolates sampled in the food-industry. | the aim of this study was to analyze the cleaning efficiency of polysaccharidases and proteolytic enzymes against biofilms of bacterial species found in food industry processing lines and to study enzyme effects on the composition of extracellular polymeric substances (eps) and biofilm removal in a clean-in-place (cip) procedure. the screening of 7 proteases and polysaccharidases for removal of biofilms of 16 bacterial species was first evaluated using a microtiter plate assay. the alkaline ph b ... | 2010 | 20198521 |
| monoclonal antibodies against recombinant hemolysin bl complex of bacillus cereus. | a three component complex system, designated hemolysin bl (hbl), is believed to be the major diarrheal toxin of bacillus cereus. identification of hbl toxin by immunoassay is advantageous over pcr as it detects the expressed form of the gene, thereby differentiating pathogenic strains from nonpathogenic strains. however, most of the immunoassays, like the bcet rpla kit, are based on the utilization of polyclonal antisera, which show cross-reactivity at times with other bacillus species. the use ... | 2010 | 20199155 |
| in vitro antimicrobial activity and chemical composition of the essential oil of foeniculum vulgare mill. | in our study, four samples of volatile oil from foeniculum vulgare, cultivated in different pedoclimatic conditions, were investigated for their antimicrobial activity and chemical composition. | 2008 | 20201277 |
| synergistic effects of ethanol and uv radiation to reduce levels of selected foodborne pathogenic bacteria. | the purpose of this study was to determine whether combined treatments would produce synergistic disinfection effects on food products during food processing compared with single treatments. we investigated the bactericidal effects of a commercial chemical disinfectant (ethanol) and of uv radiation on bacillus cereus f4810/72, cronobacter sakazakii kctc 2949, staphylococcus aureus atcc 35556, escherichia coli atcc 10536, and salmonella enterica typhimurium no/na in vitro. various concentrations ... | 2010 | 20202345 |
| hemolysis induced by bacillus cereus sphingomyelinase. | bacillus cereus sphingomyelinase (bc-smase) induces hemolysis of sheep erythrocytes which contain large amounts of sphingomyelin. we investigated the mechanism of this hemolysis in comparison to that induced by clostridium perfringens alpha-toxin. pertussis toxin, a gi-specific inhibitor, n-oleoylethernolamine, a ceramidase inhibitor, and dihydrosphingosine, a sphingosine kinase inhibitor, did not inhibit the hemolysis by bc-smase, but did inhibit that by alpha-toxin. bc-smase broadly bound to w ... | 2010 | 20214877 |
| phenol degradation by bacillus cereus: pathway and kinetic modeling. | the microbial degradation of phenol by pure cultures bacillus cereus mtcc 9817 strain akg1 and b. cereus mtcc 9818 strain akg2 is studied in batch mode for several initial concentrations of phenol in the range of 100-2000 mg/l with an interval of 100mg/l. degradation pathways are investigated at initial phenol concentrations of 100, 500, 1000, 1500, and 2000 mg/l. the bacteria are able to degrade phenol of concentration as high as 2000 mg/l. the maximum degradation rate is obtained at an initial ... | 2010 | 20219360 |
| structural investigation of transcriptional regulator hlyiir: influence of a disordered region on protein fold and dimerization. | b. cereus hlyiir belongs to the tetr family of dimeric transcriptional regulators. unlike other members of the tetr family, hlyiir contains an insert between alpha-helices alpha8 and alpha9, which is located at the subunit-subunit interface. n-terminal segment of this insert (amino acids, pro161-ser169) forms a short alpha-helix alpha8* that occupies a complementary cavity on the surface of the adjacent subunit, whereas the c-terminal segment comprising 16 amino acids (leu170-glu185) is disorder ... | 2010 | 20225260 |
| persistence strategies of bacillus cereus spores isolated from dairy silo tanks. | survival of bacillus cereus spores of dairy silo tank origin was investigated under conditions simulating those in operational dairy silos. twenty-three strains were selected to represent all b. cereus isolates (n = 457) with genotypes (rapd-pcr) that frequently colonised the silo tanks of at least two of the sampled eight dairies. the spores were studied for survival when immersed in liquids used for cleaning-in-place (1.0% sodium hydroxide at ph 13.1, 75 degrees c; 0.9% nitric acid at ph 0.8, ... | 2010 | 20227599 |
| the prevalence and concentration of bacillus cereus in retail food products in brisbane, australia. | illness associated with bacillus cereus may be underreported as very few of those affected seek medical attention owing to the mild nature and short duration of symptoms. for this reason there is little information on the prevalence and concentration of this pathogen in retail food products. a total of 1263 retail food samples were examined for b. cereus using the australian standard 1766.2.6 (1991): spread plate technique on polymyxin pyruvate egg yolk mannitol bromothymol blue agar, of which t ... | 2010 | 20230270 |
| cwpfm (entfm) is a bacillus cereus potential cell wall peptidase implicated in adhesion, biofilm formation, and virulence. | bacillus cereus entfm displays an nlpc/p60 domain, characteristic of cell wall peptidases. the protein is involved in bacterial shape, motility, adhesion to epithelial cells, biofilm formation, vacuolization of macrophages, and virulence. these data provide new information on this, so far, poorly studied toxin and suggest that this protein is a cell wall peptidase, which we propose to rename cwpfm. | 2010 | 20233921 |
| the potential pathogenicity of bacillus cereus and its relationship to bacillus anthracis. | 1947 | 20255154 | |
| [influence of permafrost microorganisms on the quality and duration of life of laboratory animals]. | microorganisms of genus bacillus strain 3m (permafrost microorganisms--pm), allocated from the samples of permafrost soils from mamontova mountain (yakutia) are influenced by the quality and duration of life of elderly mice. pms were injected intraperitoneally to cba mice at the age of 17 months. motion activity, muscular force (lifting of cargo test), food instinct activity (dosed starvation test) and cellular immunity activity (reaction of delayed-type hypersensitivity in vivo) of animals have ... | 2010 | 20297696 |
| mechanism of killing of spores of bacillus cereus and bacillus megaterium by wet heat. | to determine the mechanism of wet heat killing of spores of bacillus cereus and bacillus megaterium. | 2010 | 20302598 |
| differentiation between probiotic and wild-type bacillus cereus isolates by antibiotic susceptibility test and fourier transform infrared spectroscopy (ft-ir). | animal feed often contains probiotic bacillus strains used as feed additives. spores of the non-pathogenic b. cereus var. toyoi (product name toyocerin) are used. distinguishing between toxic wild-type bacillus cereus strains and this probiotic strain is essential for evaluating the quality and risk of feed. bacillus cereus cip 5832 (product name paciflor was used as probiotic strain until 2001. the properties of the two probiotic strains are quite similar. differentiating between probiotic stra ... | 2010 | 20303194 |
| bisphenol a removal by the dracaena plant and the role of plant-associating bacteria. | dracaena sanderiana and dracaena fragrans plants, as representatives of native, tropical, evergreen plants with fibrous root systems, were evaluated for bisphenol a (bpa) tolerance and uptake capability. d. sanderiana demonstrated significantly higher bpa removal capability than d. fragrans. therefore, it was chosen for further study. d. sanderiana tolerated bpa toxicity levels up to 80 microm, while higher bpa concentrations damaged the plant. in the sterile hydroponic system with an initial bp ... | 2010 | 20304555 |
| an assessment of the antibacterial activity in larval excretion/secretion of four species of insects recorded in association with corpses, using lucilia sericata meigen as the marker species. | the relative antibacterial activities of excretion/secretion (es) from two carrion-feeding insects, calliphora vicina robineau-desvoidy and dermestes maculatus degeer, and a detritivore, tenebrio molitor linnaeus, were compared to that of lucilia sericata meigen, a species with es of known antibacterial capacity, in order to explore the antimicrobial potential of other carrion and detritivore species. viable counts were used to assess time-kill of es against five bacterial species, staphylococcu ... | 2010 | 20307342 |
| [preparation of a 96-microwell plate dna diagnostic chip for detection of foodborne bacteria and its application in an incident of food poisoning]. | to develop a 96-microwell plate dna diagnostic chip for simultaneous detection of 9 major foodborne bacteria. | 2010 | 20335099 |
| antimicrobial, cytotoxicity and phytochemical screening of jordanian plants used in traditional medicine. | antimicrobial activity and cytotoxicity of fifty one extracts of different parts of 14 plants were studied. ethanol, methanol, aqueous, butanol, and n-hexane extracts were tested against three gram negative, two gram positive bacteria, and two fungi. cytotoxicity and phytochemical screening were determined using mtt and tlc assays, respectively. of the fifty one extracts, twenty two showed activities against different microorganisms with mics ranging from 62.5 to 1000 microg/ml. the highest acti ... | 2010 | 20336015 |
| expression of alkaline proteinase gene in two recombinant bacillus cereus feather-degrading strains. | two bacillus cereus feather-degrading strains (23/1 and 6/2) were transformed using a recombinant plasmid p5.2 carrying the alkaline proteinase gene (apre). a high level of the apre gene expression was observed when the recombinant strains were grown on sporulation medium. the expression of the apre gene proceeded during the early stationary phase and the p5.2 plasmid was segregationally and structurally stable in both strains. the two recombinant strains grown on a mineral medium with 1 % chick ... | 2010 | 20336500 |
| competitive exclusion as a mode of action of a novel bacillus cereus aquaculture biological agent. | to determine the contribution of potential modes of action of a bacillus cereus aquaculture biological control agent in inhibition of the fish pathogen, aeromonas hydrophila. | 2010 | 20337929 |
| the antagonistic effect of bacillus cereus. | 1947 | 20344600 | |
| comparative transcriptomic and phenotypic analysis of the responses of bacillus cereus to various disinfectant treatments. | antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. however, the cellular response of bacteria to various disinfectants is unclear. in this study, the physiological and genome-wide transcriptional responses of bacillus cereus atcc 14579 exposed to four different disinfectants (benzalkonium chloride, sodium hypochlorite, hydrogen peroxide, and peracetic acid) were analyzed. for each disinfectant, concentrations leading to the attenuation of growth, growth a ... | 2010 | 20348290 |
| peptides panned from a phage-displayed random peptide library are useful for the detection of bacillus anthracis surrogates b. cereus 4342 and b. anthracis sterne. | recent use of bacillus anthracis as a bioweapon has highlighted the need for a sensitive monitoring system. current bacterial detection tests use antibodies as bio-molecular recognition elements which have limitations with regard to time, specificity and sensitivity, creating the need for new and improved cost-effective high-affinity detection probes. in this study, we screened a commercially available bacteriophage-displayed random peptide library using bacillus cereus 4342 cells as bait to ide ... | 2010 | 20350526 |
| fulminant sepsis caused by bacillus cereus in patients with hematologic malignancies: analysis of its prognosis and risk factors. | bacillus cereus is a growing concern as a cause of life-threatening infections in patients with hematologic malignancies. however, the risk factors for patients with unfavorable outcomes have not been fully elucidated. at our institution, we observed the growth of b. cereus in blood culture in 68 patients with (23) or without (45) hematologic malignancies treated from september 2002 to november 2009. we defined a case as having sepsis when more than two blood culture sets were positive for b. ce ... | 2010 | 20367571 |
| expanding the known repertoire of virulence factors produced by bacillus cereus through early secretome profiling in three redox conditions. | the pathogen bacillus cereus causes diarrheal disease in humans. in the small intestine, b. cereus has to deal with anaerobiosis, low oxidoreduction potential, and carbohydrate limitation conditions. to gain insight into the virulence potential of low density b. cereus cells in such an environment, we cultured bacteria in low and high oxidoreduction potential anoxic conditions and in fully oxic conditions and compared their full secretomes. a unique pattern of proteins assigned to virulence fact ... | 2010 | 20368289 |
| removal of sediment and bacteria from water using green chemistry. | although nearly all newly derived water purification methods have improved the water quality in developing countries, few have been accepted and maintained for long-term use. field studies indicate that the most beneficial methods use indigenous resources, as they are both accessible and accepted by communities they help. in an effort to implement a material that will meet community needs, two fractions of mucilage gum were extracted from the opuntia ficus-indica cactus and tested as flocculatio ... | 2010 | 20369814 |
| characterization of bacterial spore germination using integrated phase contrast microscopy, raman spectroscopy, and optical tweezers. | we present a methodology that combines external phase contrast microscopy, raman spectroscopy, and optical tweezers to monitor a variety of changes during the germination of single bacillus cereus spores in both nutrient (l-alanine) and non-nutrient (ca-dipicolinic acid (dpa)) germinants with a temporal resolution of approximately 2 s. phase contrast microscopy assesses changes in refractility of individual spores during germination, while raman spectroscopy gives information on changes in spore ... | 2010 | 20369827 |
| insertional mutagenesis reveals genes involved in bacillus cereus atcc 14579 growth at low temperature. | transposon mutagenesis of bacillus cereus atcc 14579 yielded cold-sensitive mutants. mutants of genes encoding enzymes of the central metabolism were affected by cold, but also by other stresses, such as ph or salt, whereas a mutant with transposon insertion in the promoter region of bc0259 gene, encoding a putative dead-box rna helicase displaying homology with escherichia coli csda and bacillus subtilis csha rna helicases, was only cold-sensitive. expression of the bc0259 gene at 10 degrees c ... | 2010 | 20370835 |
| phytochemical and biological investigations of elaeodendron schlechteranum. | elaeodendron schlechteranum (loes.) loes. is a shrub or tree belonging to the family celastraceae. in tanzania, in addition to ethnopharmacological claims in treating various non-infectious diseases, the root and stem bark powder is applied on septic wounds, and the leaf paste is used for treatment of boils and carbuncles. the aim of this study was to identify the putative active constituents of the plant. | 2010 | 20371284 |
| bacillus cereus, a volatile human pathogen. | bacillus cereus is a gram-positive aerobic or facultatively anaerobic, motile, spore-forming, rod-shaped bacterium that is widely distributed environmentally. while b. cereus is associated mainly with food poisoning, it is being increasingly reported to be a cause of serious and potentially fatal non-gastrointestinal-tract infections. the pathogenicity of b. cereus, whether intestinal or nonintestinal, is intimately associated with the production of tissue-destructive exoenzymes. among these sec ... | 2010 | 20375358 |
| rapid degradation of n-3-oxo-acylhomoserine lactones by a bacillus cereus isolate from malaysian rainforest soil. | a bacterial strain, km1s, was isolated from a malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. km1s was clustered closely to bacillus cereus by 16s ribosomal dna sequence analysis. it degraded n-3-oxo-hexanoyl homoserine lactone and n-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 microg ahl h(-1) per 10(9) cfu/ml, respectively, as determined by the rapid resolution liquid chromatograp ... | 2010 | 20376561 |
| efficient amplification of genes involved in microbial secondary metabolism by an improved genome walking method. | genome walking is a commonly used technique for the identification of dna sequences adjacent to known regions. despite the development of various genome walking methods, nonspecific products are often produced in certain circumstances, especially when gc-rich dna sequences are dealt with. to effectively resolve such technical issues, a simple nested polymerase chain reaction-based genome walking method has been developed by implementing a progressively decreased annealing temperature from 70 deg ... | 2010 | 20376630 |
| biodegradation of phenol in batch culture by pure and mixed strains of paenibacillus sp. and bacillus cereus. | the bacterial strains, paenibacillus sp. (ay952466) and bacillus cereus (dq002384), have proven capacity to degrade lignin and pentachlorophenol. in the present study, both strains were screened at different concentrations of phenol on mineral salt agar medium in the presence of glucose. at optimized condition (ph 7.5 +/- 0.2, 37 +/- 1 degrees c, 120 rpm, 1% glucose w/v), it was observed that both paenibacillus sp., b. cereus and its mixed culture degraded phenol (500 mg/l) up to 53.86%, 91.63% ... | 2009 | 20380142 |
| decolorization of water soluble azo dyes by bacterial cultures, isolated from dye house effluent. | the aim of this work is to isolate and characterize bacterial isolates form dye house effluent, and to check their ability of decolorizing sulfonated azo dyes, and also to study influence of various environmental parameters on same process. among seven gram positive bacterial isolates obtained form dye house effluent, m1 (bacillus cereus) and m6 were proved to be more potent for decolorizing sulfonated azo dyes under aerobic conditions. maltose as carbon source and peptone as nitrogen source enh ... | 2010 | 20392633 |
| an integrated, self-contained microfluidic cassette for isolation, amplification, and detection of nucleic acids. | a self-contained, integrated, disposable, sample-to-answer, polycarbonate microfluidic cassette for nucleic acid-based detection of pathogens at the point of care was designed, constructed, and tested. the cassette comprises on-chip sample lysis, nucleic acid isolation, enzymatic amplification (polymerase chain reaction and, when needed, reverse transcription), amplicon labeling, and detection. on-chip pouches and valves facilitate fluid flow control. all the liquids and dry reagents needed for ... | 2010 | 20401537 |
| [effect of phytosubstances with antioxidant activity on persistence properties of microorganisms]. | the in vitro effect of phytosubstances containing flavonoids and phenylpropanoids on the antilysozyme activity of varioous groups of microorganisms, such as staphylococci, bacilli, enterobacteria and nonfermenting bacteria was studied. the second fraction of the pink carbonic acid extract showed high antioxidant activity and most efficiently inhibited the ability of various microorganisms to inactivate the host lysozyme. the selected phytosubstance could be useful in clinical practice as an addi ... | 2009 | 20415257 |
| blechnum orientale linn - a fern with potential as antioxidant, anticancer and antibacterial agent. | blechnum orientale linn. (blechnaceae) is used ethnomedicinally for the treatment of various skin diseases, stomach pain, urinary bladder complaints and sterilization of women. the aim of the study was to evaluate antioxidant, anticancer and antibacterial activity of five solvent fractions obtained from the methanol extract of the leaves of blechnum orientale linn. | 2010 | 20429956 |
| [screening of collagenase-producing strain and purification of bacillus cereus collagenase]. | we isolated the strain mbl13 with high collagenase productivity from the soil of piled up animal bones. it was identified as bacillus cereus. we purified and characterized bacillus cereus collagenase (bcc). the molecular weight of bcc was 38.0 kda and the optimum temperature and ph for the enzyme activity were 40 degrees c and 8.0 respectively. the enzyme was stable when the temperature was below 50 degrees c, but only retained 10% activity when kept at 60 degrees c for 1 h. the enzyme activity ... | 2010 | 20432938 |
| studies of the commitment step in the germination of spores of bacillus species. | spores of bacillus species are said to be committed when they continue through nutrient germination even when germinants are removed or their binding to spores' nutrient germinant receptors (grs) is both reversed and inhibited. measurement of commitment and the subsequent release of dipicolinic acid (dpa) during nutrient germination of spores of bacillus cereus and bacillus subtilis showed that heat activation, increased nutrient germinant concentrations, and higher average levels of grs/spore s ... | 2010 | 20435722 |
| antibacterial properties of grapefruit seed extract against paenibacillus larvae subsp. larvae. | twenty-one samples of grapefruit seed extract (gse) either from marketed products or provided by an apiculturist were analysed to verify their inhibition activity, in particular against paenibacillus larvae subsp. larvae, responsible for american foulbrood. the bactericide capacity of gse has been measured in bacillus subtilis bga, bacillus cereus 11778, bacillus cereus k250 and micrococcus luteus 9341a; these bacteria are normally used in the laboratory to study inhibitors. the results showed t ... | 2004 | 20437392 |
| food poisoning associated with emetic-type of bacillus cereus in korea. | bacillus cereus can cause diarrheal and emetic types of food poisoning but little study has been done on emetic type of food poisoning in korea. the objective of this study was to report on the emetic type of food poisoning associated with b. cereus in korea. the toxin gene profile, toxin production, and antibiotic resistance of b. cereus isolates were investigated in this study. b. cereus was detected in three out of four samples, while the other food poisoning bacteria were not detected. all i ... | 2010 | 20446859 |
| novel 6,8-dibromo-4(3h)quinazolinone derivatives of anti-bacterial and anti-fungal activities. | starting from 4-(6,8-dibromo-2-phenyl-4-oxo-(4h)-quinazolin-3-yl)-benzoic acid ethyl ester (ii) and its acid hydrazide iii, a new series of schiff bases iv and their cyclized products, thiazolidinones v, oxadiazole viii, pyrazoles x-xii, pyrroles xiii-xv and other related products were synthesized. these compounds were screened for their anti-bacterial activity against gram-positive bacteria (staphylococcus aureus, legionella monocytogenes and bacillus cereus) and gram-negative bacteria (escheri ... | 2010 | 20452707 |
| quantification of the effect of culturing temperature on salt-induced heat resistance of bacillus species. | short- and long-term exposure to mild stress conditions can activate stress adaptation mechanisms in pathogens, resulting in a protective effect toward otherwise lethal stresses. the mesophilic strains bacillus cereus atcc 14579 and atcc 10987 and the psychrotolerant strain b. weihenstephanensis kbab4 were cultured at 12 degrees c and 30 degrees c until the exponential growth phase (i) in the absence of salt, (ii) in the presence of salt, and (iii) with salt shock after they reached the exponent ... | 2010 | 20453152 |
| the characterization and diversity of bacterial endophytes of grapevine. | the diversity of culturable and nonculturable bacterial endophytes of grapevine (vitis vinifera l.) was examined using a combination of cultivation and molecular methods. entire grapevines were sampled to characterize bacterial diversity from different locations throughout the vine. gas chromatography of fatty acid methyl esters (fames) was used to identify culturable isolates prior to subsequent further microbiological characterization, whilst denaturing gradient gel electrophoresis (dgge) was ... | 2010 | 20453907 |
| manipulation of thiocillin variants by prepeptide gene replacement: structure, conformation, and activity of heterocycle substitution mutants. | bacillus cereus atcc 14579 converts the c-terminal 14 residues of a 52-mer prepeptide into a related set of eight variants of the thiocillin subclass of thiazolyl peptide antibiotics by a cascade of post-translational modifications that alter 13 of those 14 residues. we have introduced prepeptide gene variants into a knockout strain to conduct an alanine scan of all 14 progenitor residues, as well as a serine scan of the six cysteine residues that are converted to thiazoles in the mature natural ... | 2010 | 20455532 |
| antioxidant, antibacterial and antigiardial activities of walsura robusta roxb. | walsura robusta roxb. (family: meliaceae) is a well-known multi-purpose medicinal plant, and has been employed for a wide range of disease conditions without documented scientific data. in the current study, four pure isolated compounds, 3,4,5-trimethoxyphenyl beta-d-glucopyranoside (1), turpinionoside a (2), (+)-lyoniresinol 3alpha-o-beta-d-glucopyranoside (3) and (-)-lyoniresinol 3alpha-o-beta-d-glucopyranoside (4), were isolated from the leaves and twigs of w. robusta. biological evaluation f ... | 2010 | 20461627 |
| siderophore-mediated iron acquisition in bacillus anthracis and related strains. | recent observations have shed light on some of the endogenous iron-acquisition mechanisms of members of the bacillus cereus sensu lato group. in particular, pathogens in the b. cereus group use siderophores with both unique chemical structures and biological roles. this review will focus on recent discoveries in siderophore biosynthesis and biology in this group, which contains numerous human pathogens, most notably the causative agent of anthrax, bacillus anthracis. | 2010 | 20466767 |
| antimicrobial activity and essential oil composition of a new t. argyrophyllum (c. koch) tvzel var. argyrophyllum chemotype. | water-distilled essential oils from flowers and stems of tanacetum argyrophyllum var. argyrophyllum from turkey were analyzed by gc and gc/ms. the flower oil of t. argyrophyllum var. argyrophyllum was characterized with camphor 29.7%, borneol 12.0%, 1,8-cineole 8.4% and bornyl acetate 6.1%. stem oil was characterized with camphor 26.6%, 1,8-cineole 17.5%, and borneol 15.0%. our previous research and literature on the essential oil of this plant reported oils with high thujone content unlike the ... | 2010 | 20484836 |
| rapid multi-locus sequence typing using microfluidic biochips. | multiple locus sequence typing (mlst) has become a central genotyping strategy for analysis of bacterial populations. the scheme involves de novo sequencing of 6-8 housekeeping loci to assign unique sequence types. in this work we adapted mlst to a rapid microfluidics platform in order to enhance speed and reduce laboratory labor time. | 2010 | 20485679 |
| polymyxin-direct hemoperfusion for sepsis-induced multiple organ failure. | we report a case of multiple organ failure caused by the bacillus cereus infection during acute lymphoblastic leukemia therapy, who was treated successfully. a 15-year-old male developed (b. cereus) sepsis on the 19th day after chemotherapy initiation. polymyxin-direct hemoperfusion for septic shock was started, followed by continuous hemodiafiltration. his condition improved after starting the hemoperfusion. at the onset of sepsis, elevated levels of serum inflammatory cytokines, anti-inflammat ... | 2010 | 20486188 |
| action of phospholipase c (bacillus cereus) on isolated myelin sheath preparations. | the action of phospholipase c (bacillus cereus) on the phospholipids of myelin sheath preparations has been investigated. with freshly isolated bovine brain myelin about 40% of the total phospholipid could be hydrolyzed by this enzyme. with bovine spinal cord myelin the phospholipid seemed more resistant to attack whereas the opposite was the case with myelin from guinea-pig brain or rat brain. with fresh bovine brain myelin, phosphatidylcholine and the ethanolamine-containing phospholipids were ... | 1984 | 20488038 |
| cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract. | cadmium (cd²(+)), a naturally occurring heavy metal, is an important environmental pollutant and a potent toxicant to bacteria. the gastrointestinal (gi) tract microflora has a marked capacity to cope with the increased load of ingested metals. however, heavy metals may have harmful effects on git microflora. under the conditions of experimental exposure to cadmium, changes in the population of intestinal microflora in healthy mice were examined. five experimental groups received 23 to 50 mg kg⁻ ... | 2011 | 20488844 |
| survey on the microbiological quality of chinese food preparations. | the microbiological quality of 118 samples of traditional chinese food from restaurants and take-away establishments in the provinces of padua, treviso, and venice was examined (april-july, 2008). these food items included various ready-to-eat products. the microbiological quality of the majority of these food items was acceptable; only 12% of samples had values greater than or equal to 6 log cfu/g (eight items were salads) and 19% of samples had values greater than 500 cfu fecal coliforms/g (p ... | 2010 | 20490663 |
| lmbe proteins from bacillus cereus are de-n-acetylases with broad substrate specificity and are highly similar to proteins in bacillus anthracis. | the genomes of bacillus cereus and its closest relative bacillus anthracis each contain two lmbe protein family homologs: bc1534 (ba1557) and bc3461 (ba3524). only a few members of this family have been biochemically characterized including n-acetylglucosaminylphosphatidyl inositol (glcnac-pi), 1-d-myo-inosityl-2-acetamido-2-deoxy-alpha-d-glucopyranoside (glcnac-ins), n,n'-diacetylchitobiose (glcnac(2)) and lipoglycopeptide antibiotic de-n-acetylases. all these enzymes share a common feature in ... | 2010 | 20491912 |
| characterization of the properties of pediococcus parvulus for probiotic or protective culture use. | pediococcus parvulus 2.6 (previously pediococcus damnosus 2.6, here confirmed as p. parvulus by 16s dna sequencing) displayed antibacterial activity toward several bacterial species, including isolates found as contaminants in oats, herein genetically identified as bacillus cereus. no inhibition of listeria monocytogenes was found under the conditions used. antibacterial activity was retrieved after ammonium sulfate or acetone precipitation showed it to be peptide mediated. p. parvulus 2.6 has p ... | 2010 | 20501049 |
| synthesis and pharmacological evaluation of schiff bases of 4-(2-aminophenyl)-morpholines. | in the present study, a novel series of 4-(2-aminophenyl)morpholines were synthesized and characterized by ir, (1)h-nmr, (13)c nmr and mass spectral analysis. the synthesized compounds were screened for analgesic (100 and 200 mg/kg), antiinflammatory (200 and 400 mg/kg), antibacterial (bacillus subtilis, bacillus cereus, staphylococcus epidermidis, staphylococcus aureus, klebsiella pneumoniae, pseudomonas aeruginosa and escherichia coli) and antifungal (candida albicans and aspergillus niger) ac ... | 2009 | 20502549 |
| environmental factors determining the epidemiology and population genetic structure of the bacillus cereus group in the field. | bacillus thuringiensis (bt) and its insecticidal toxins are widely exploited in microbial biopesticides and genetically modified crops. its population biology is, however, poorly understood. important issues for the safe, sustainable exploitation of bt include understanding how selection maintains expression of insecticidal toxins in nature, whether entomopathogenic bt is ecologically distinct from related human pathogens in the bacillus cereus group, and how the use of microbial pesticides alte ... | 2010 | 20502683 |
| rapid analysis of gram-positive bacteria in water via membrane filtration coupled with nanoprobe-based maldi-ms. | matrix-assisted laser desorption/ionization (maldi) mass spectrometry (ms) is challenging when it is directly applied to identify bacteria in water. this study demonstrates a rapid, sensitive, and selective technique for detection of gram-positive bacteria in water. it involves a combination of membrane filtration (mf) and vancomycin-conjugated magnetite nanoparticles (vnps) to selectively separate and concentrate gram-positive bacteria in tap water and reservoir water, followed by rapid analysi ... | 2010 | 20512317 |
| essential oil composition and antibacterial activity of tanacetum argenteum (lam.) willd. ssp. argenteum and t. densum (lab.) schultz bip. ssp. amani heywood from turkey. | water-distilled essential oils from aerial parts of tanacetum argenteum ssp. argenteum and t. densum ssp. amani from turkey were analyzed by gc and gc/ms. the essential oil of t. argenteum ssp. argenteum was characterized with alpha-pinene 36.7%, beta-pinene 27.5% and 1,8-cineole 9.8%. t. densum ssp. amani was characterized with beta-pinene 27.2%, 1,8-cineole 13.1%, alpha-pinene 9.7% and p-cymene 8.9%. antibacterial activity of the oils were evaluated for five gram-positive and five gram-negativ ... | 2010 | 20513969 |
| [bacillus cereus keratitis--case report]. | bacillus cereus is a gram-positive, rod-shaped, beta hemolytic bacterium which rarely causes eye infections. we present a case of 15 year old boy with unilateral keratitis. he was treated for two months by his ophthalmologist who suspected herpetic keratitis. patient most probably suffered secondary infection by bacillus cereus. after bacillus was identified and targeted treatment given clinical picture improved rapidly. until now there are only few cases of bacillus cereus keratitis published. | 2010 | 20521509 |
| composition and antimicrobial activity of the essential oils from flowers of senecio othonnae, s. racemosus, and s. nemorensis. | the chemical composition of the essential oils of senecio othonnae, s. racemosus, and s. nemorensis obtained by hydrodistillation of fresh flowers were analysed by gc-fid and gc-ms. a total of seventy-six compounds were identified, constituting over 83.1%, 97.7% and 86.8% of the total oil composition of the flowers of s. othonnae, s. racemosus, and s. nemorensis. the main components of the oils were caryophyllene oxide (18.6%), (e)-beta-farnesene (21.6%), and gamma-curcumene (42.8%), respectivel ... | 2010 | 20521557 |
| biosynthesis of a new udp-sugar, udp-2-acetamido-2-deoxyxylose, in the human pathogen bacillus cereus subspecies cytotoxis nvh 391-98. | we have identified an operon and characterized the functions of two genes from the severe food-poisoning bacterium, bacillus cereus subsp. cytotoxis nvh 391-98, that are involved in the synthesis of a unique udp-sugar, udp-2-acetamido-2-deoxyxylose (udp-n-acetyl-xylosamine, udp-xylnac). uglcnacdh encodes a udp-n-acetyl-glucosamine 6-dehydrogenase, converting udp-n-acetylglucosamine (udp-glcnac) to udp-n-acetyl-glucosaminuronic acid (udp-glcnaca). the second gene in the operon, uxnacs, encodes a ... | 2010 | 20529859 |
| an organic solvent-stable protease from organic solvent-tolerant bacillus cereus wq9-2: purification, biochemical properties, and potential application in peptide synthesis. | an extracellular solvent-stable protease producing bacterium wq9-2 was isolated and identified taxonomically as bacillus cereus. the protease from strain wq9-2 was purified to homogeneity with an estimated molecular mass of 37kda. the purified protease showed maximum activity at 50 degrees c and ph 8.0. the protease may be classified as a metalloprotease since it was strongly inhibited by edta and 1,10-phenanthroline. the protease showed extreme activity and stability in the presence of both 50% ... | 2010 | 20541397 |
| dissemination of bacillus cereus in the burn unit due to contaminated non-sterile gloves. | 2010 | 20542594 | |
| a novel spore protein, exsm, regulates formation of the exosporium in bacillus cereus and bacillus anthracis and affects spore size and shape. | bacillus cereus spores are assembled with a series of concentric layers that protect them from a wide range of environmental stresses. the outermost layer, or exosporium, is a bag-like structure that interacts with the environment and is composed of more than 20 proteins and glycoproteins. here, we identified a new spore protein, exsm, from a beta-mercaptoethanol extract of b. cereus atcc 4342 spores. subcellular localization of an exsm-green fluorescent protein (gfp) protein revealed a dynamic ... | 2010 | 20543075 |
| isolation and identification of bacillus spp. and related genera from different starchy foods. | samples of butternut squash, potatoes, rice, and wheat flour were analyzed. bacillus spp. and related species belonging to paenibacillus and brevibacillus genera were found in 96% of the samples. in butternut squash, predominant species were bacillus pumilus and paenibacillus polymyxa together with other bacillus spp. species (b. cereus, b. licheniformis, b. sphaericus, and b. subtilis). in all the potato samples, bacillus species were detected (b. cereus, b. mycoides, and b. licheniformis). als ... | 2010 | 20546413 |
| microbial growth in dry grain food (sunsik) beverages prepared with water, milk, soymilk, or honey-water. | this study was conducted to investigate the growth of microorganisms, including pathogenic bacteria such as cronobacter sakazakii and bacillus cereus, in sunsik beverages made of water, milk, soymilk, or honey-water during storage at room temperature. prepared sunsik beverages were stored at room temperature and the growth of total aerobic counts, escherichia coli/coliforms, and yeast and mold were measured. also, samples inoculated with a cocktail of c. sakazakii or b. cereus spores were stored ... | 2010 | 20546416 |
| isolation and characterization of a novel analyte from bacillus subtilis sc-8 antagonistic to bacillus cereus. | in this study, an effective substance was isolated from bacillus subtilis sc-8, which was obtained from traditionally fermented soybean paste, cheonggukjang. the substance was purified by hplc, and its properties were analyzed. it had an adequate antagonistic effect on bacilluscereus, and its spectrum of activity was narrow. when tested on several gram-negative and gram-positive foodborne pathogenic bacteria such as salmonella enterica, salmonella enteritidis, staphylococcus aureus, and listeria ... | 2010 | 20547349 |
| development of starter culture for improved processing of lafun, an african fermented cassava food product. | to select appropriate micro-organisms to be used as starter culture for reliable and reproducible fermentation of lafun. | 2010 | 20553347 |
| novel family of antimicrobial peptides from the skin of rana shuchinae. | so far numerous antimicrobial peptides have been characterized from amphibians. in this work, a new family of antimicrobial peptides, named shuchin, was purified and characterized from skin secretions of the frog, rana shuchinae that lives in freezing mountains. totally two members of shuchin (shuchin 1 and 2) were identified with the amino acid sequence of nalsmprnkcnralmcfg and nalssprnkcdrasscfg, respectively. cdnas encoding shuchins were cloned from the skin cdna library of r. shuchinae. the ... | 2010 | 20553780 |
| genome-wide single nucleotide polymorphism typing method for identification of bacillus anthracis species and strains among b. cereus group species. | as an issue of biosecurity, species-specific genetic markers have been well characterized. however, bacillus anthracis strain-specific information is currently not sufficient for traceability to identify the origin of the strain. by using genome-wide screening using short read mapping, we identified strain-specific single nucleotide polymorphisms (snps) among b. anthracis strains including japanese isolates, and we further developed a simplified 80-tag snp typing method for the primary investiga ... | 2010 | 20554827 |
| inactivation of bacillus cereus by na-chlorophyllin-based photosensitization on the surface of packaging. | this study was focused on the possibility to inactivate food-borne pathogen bacillus cereus by na-chlorophyllin (na-chl)-based photosensitization in vitro and after attachment to the surface of packaging material. | 2010 | 20557405 |
| generation of chitin-derived oligosaccharides toxic to pathogenic bacteria using chia74, an endochitinase native to bacillus thuringiensis. | to demonstrate that an endochitinase (chia74) native to bacillus thuringiensis can be used to generate chitin-derived oligosaccharides (ogs) with antibacterial activity against a number of aetiological agents of disease, including bacteria that cause diarrhoeal and emetic syndromes in humans. | 2010 | 20557451 |
| an independent in vitro comparison of povidone iodine and sterilid. | povidone iodine (pi) and sterilid (sl) are biocides used to decrease bacterial load on the eyelid margin. we compared the antibacterial activity of pi and sl to determine the better antiseptic. | 2010 | 20565315 |
| growth of microorganisms in total parenteral nutrition solutions containing lipid. | to identify the microorganisms that can grow rapidly in total parenteral nutrition (tpn) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (staphylococcus aureus, serratia marcescens, bacillus cereus, and candida albicans) in tpn solutions containing lipid. | 2010 | 20567610 |
| cereulide and valinomycin, two important natural dodecadepsipeptides with ionophoretic activities. | cereulide produced by bacillus cereus sensu stricto and valinomycin synthesized mainly by streptomyces spp. are natural dodecadepsipeptide ionophores that act as potassium transporters. moreover, they comprise three repetitions of similar tetrapeptide motifs synthesized by nonribosomal peptide synthesis complexes. resemblances in their structure find their reflections in the same way of action. the toxicity of valinomycin and cereulide is an effect of the disturbance of ionic equilibrium and tra ... | 2010 | 20568524 |
| response of bacillus cereus to gamma-irradiation in combination with carvacrol or mild heat treatment. | carvacrol and mild heat treatment were tested for their efficiency to increase the radiosensitivity of bacillus cereus in broth. the bacterium was treated with gamma-irradiation alone or in combination with carvacrol at its minimal inhibitory concentration or mild heat treatment for 10 min at 45 degrees c. the effects of this combination of treatments were studied on various parameters: the bacterial viability, the modifications of the cell morphology with scanning electron microscopy (sem), the ... | 2010 | 20568774 |
| cell-based biosensor for rapid screening of pathogens and toxins. | development and validation of a mammalian cell-based biosensor for application in food defense and food safety was investigated. three prototypes of the biosensor capable of handling different sample types were developed and tested with food and beverages. the sensing element is a b lymphocyte ped-2e9 cell-line, encapsulated in collagen matrix in 3d scaffold. the uniqueness of this biosensor is that it detects analyte interaction with mammalian cells and is able to distinguish pathogenic from no ... | 2010 | 20570502 |
| modelling the influence of palmitic, palmitoleic, stearic and oleic acids on apparent heat resistance of spores of bacillus cereus ntcc 11145 and clostridium sporogenes pasteur 79.3. | heat resistance of spores is affected by many factors such as temperature, ph, water activity (aw) and others. previous studies have reported that free fatty acids can affect the germination and growth of bacterial spores. in this study, we investigated the influence of free fatty acids in heating medium or in recovery medium on the heat resistance of spores of bacillus cereus ntcc 11145 and clostridium sporogenes pasteur 79.3. four free fatty acids were studied: palmitic, palmitoleic, stearic a ... | 2010 | 20573415 |
| hydrolysis of organophosphate esters: phosphotriesterase activity of metallo-beta-lactamase and its functional mimics. | the phosphotriesterase (pte) activity of a series of binuclear and mononuclear zinc(ii) complexes and metallo-beta-lactamase (mbetal) from bacillus cereus was studied. the binuclear complex 1, which exhibits good mbetal activity, shows poor pte activity. in contrast, complex 2, a poor mimic of mbetal, exhibits much higher activity than 1. the replacement of cl(-) ligands by oh(-) is important for the high pte activity of complex 2 because this complex does not show any catalytic activity in meth ... | 2010 | 20575118 |
| antioxidant and antimicrobial activity of alpinia officinarum. | alpinia officinarum is a rhizome belonging to the family zingeberaeceae. hydro alcoholic extract by hot and cold maceration and methanol extract by percolation process qualitative phytochemical analysis of extract of alpinia officinarum rhizome showed a majority of the compound including tannins, alkaloids, flavonoids and saponins. hydroalcoholic extract prepared by hot maceration process was found to contain more phenol and flavonol and it was measured as 50.1 mg/g and 54.02 mg/g, respectively. ... | 2010 | 20582210 |
| triplex pcr-based detection of enterotoxigenic bacillus cereus atcc 14579 in nonfat dry milk. | although many strains of bacillaceae are considered nonpathogenic, bacillus cereus is recognized worldwide as a bacterial pathogen in a variety of foods. the ability of b. cereus to cause gastroenteritis following ingestion of contaminated food is due to the production of enterotoxins. the ubiquity of this genus makes it a persistent problem for quality assurance in food processing environments. the primary objective of this study was to develop and apply a multiplex real-time pcr-based assay fo ... | 2011 | 20586065 |
| high abundance and diversity of bacillus anthracis plasmid pxo1-like replicons in municipal wastewater. | water from the influent of a municipal wastewater treatment plant as well as soil samples collected from the shoreline of 10 lakes were screened for the presence of the bacillus anthracis pxo1-like plasmid replicon repx using a pcr assay. specific pcr products were retrieved from all samples, indicating a widespread presence of pxo1-like plasmid replicons in various environmental settings. initial screening by restriction enzyme analysis revealed at least two forms of the repx gene in the wastew ... | 2010 | 20597981 |
| identification, genetic diversity and cereulide producing ability of bacillus cereus group strains isolated from beninese traditional fermented food condiments. | bacillus cereus sensu lato is often detected in spontaneously fermented african foods but is rarely identified to species level. only some of the b. cereus group species are reported to be pathogenic to humans and identification to species level is necessary to estimate the safety of these products. in the present study, a total of 19 bacillus cereus group spp. isolated from afitin, iru and sonru, three spontaneously fermented african locust (parkia biglobosa) bean based condiments produced in b ... | 2010 | 20599285 |
| conjugal transfer between bacillus thuringiensis and bacillus cereus strains is not directly correlated with growth of recipient strains. | bacillus thuringiensis and bacillus cereus belong to the b. cereus species group. the two species share substantial chromosomal similarity and differ mostly in their plasmid content. the phylogenetic relationship between these species remains a matter of debate. there is genetic exchange both within and between these species, and current evidence indicates that insects are a particularly suitable environment for the growth of and genetic exchange between these species. we investigated the conjug ... | 2010 | 20600090 |
| aerobic biogenesis of selenium nanospheres by bacillus cereus isolated from coalmine soil. | microorganisms that are exposed to pollutants in the environment, such as metals/metalloids, have a remarkable ability to fight the metal stress by various mechanisms. these metal-microbe interactions have already found an important role in biotechnological applications. it is only recently that microorganisms have been explored as potential biofactories for synthesis of metal/metalloid nanoparticles. biosynthesis of selenium (se 0) nanospheres in aerobic conditions by a bacterial strain isolate ... | 2010 | 20602763 |
| [clinical relevance of time-to-positivity in bactec9240 blood culture system]. | continuous monitoring systems have allowed determination of the time-to-positivity (ttp). we evaluated the clinical relevance of ttp in the bactec9240 system (becton-dickinson, usa). | 2010 | 20603588 |
| crystallization and preliminary x-ray analysis of a phosphopentomutase from bacillus cereus. | phosphopentomutases (ppms) interconvert d-ribose 5-phosphate and alpha-d-ribose 1-phosphate to link glucose and nucleotide metabolism. ppm from bacillus cereus was overexpressed in escherichia coli, purified to homogeneity and crystallized. bacterial ppms are predicted to contain a di-metal reaction center, but the catalytically relevant metal has not previously been identified. sparse-matrix crystallization screening was performed in the presence or absence of 50 mm mncl(2). this strategy resul ... | 2010 | 20606280 |
| anaerobic benzene biodegradation by a pure bacterial culture of bacillus cereus under nitrate reducing conditions. | a pure culture using benzene as sole carbon and energy sources was isolated by screening procedure from gasoline contaminated soil. the analysis of the 16s rdna gene sequence, morpholpgical and physiological characteristics showed that the isolated strain was a member of genus bacillus cereus. the biodegradation performance of benzene by b. cereus was evaluated, and the results showed that benzene could be efficiently biodegraded when the initial benzene concentration was below 150 mg/l. the met ... | 2010 | 20608507 |