Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the corynebacterium diphtheriae shaft pilin spaa is built of tandem ig-like modules with stabilizing isopeptide and disulfide bonds. | cell-surface pili are important virulence factors that enable bacterial pathogens to adhere to specific host tissues and modulate host immune response. relatively little is known about the structure of gram-positive bacterial pili, which are built by the sortase-catalyzed covalent crosslinking of individual pilin proteins. here we report the 1.6-a resolution crystal structure of the shaft pilin component spaa from corynebacterium diphtheriae, revealing both common and unique features. the spaa p ... | 2009 | 19805181 |
| in vitro effects on bacterial growth of phenoloxidase reaction products. | an active phenoloxidase preparation from the freshwater crayfish pacifastacus leniusculus exhibited a strong antibacterial effect in vitro on the bacteria aeromonas hydrophila, escherichia coli, streptococcus pneumoniae whereas a weaker but still significant effect against bacillus cereus, pseudomonas aeruginosa and staphylococcus aureus. in most cases reduction of bacterial growth was stronger when dopamine was used as substrate as compared to l-dopa. the effect on bacteria was abolished if no ... | 2010 | 19808037 |
| new cyclopentenones from strain trichoderma sp. ylf-3. | two new cyclopentenones, trichodermone a (1) and trichodermone b (2), together with a known compound, 3-(3-oxocyclopent-1-enyl) propanoic acid (3), were isolated from the strain trichoderma sp. ylf-3. their structures were elucidated on the basis of their spectroscopic data. all compounds were assayed for antibacterial activity. compound 3 showed activity against staphyloccocus aureus and bacillus cereus. | 2009 | 19809916 |
| chemical composition and antimicrobial activity of the extracts of kefe cumin (laser trilobum l.) fruits from different regions. | structural properties (using gas chromatographic analyses: gas chromatography and gas chromatography-mass spectrometry) of some compounds (pure chemicals, essential oils, extracts, etc.) and systematic investigation of antimicrobial activities (with agar dilution and agar diffusion disc methods) were determined in the fruits of kefe cumin (laser trilobum l.) obtained from different regions (germany, india, and turkey). the main components were established as limonene (41.03-72.24%) and perillald ... | 2009 | 19817640 |
| cluster of necrotizing enterocolitis in a neonatal intensive care unit: new mexico, 2007. | although the cause of necrotizing enterocolitis (nec) is unknown, infection control practices have been shown to play an important role in containing many outbreaks. we investigated the etiology of a cluster of nec in a level 3 neonatal intensive care unit and monitored for new cases following the implementation of enhanced infection control measures. | 2010 | 19822381 |
| microbiological quality of saffron from the main producer countries. | a microbiological study of saffron spice was undertaken in the context of a european research project (methodologies for implementing international standards for saffron purity and quality, the acronym for which is saffic), analyzing 79 samples obtained from the main producer countries, namely greece, iran, italy, morocco, and spain. current microbiological quality criteria are the same as for other spices, but saffron is added in minute quantities during the cooking process, so the health risk ... | 2009 | 19833050 |
| the inha metalloproteases of bacillus cereus contribute concomitantly to virulence. | the virulence of bacillus cereus requires that bacteria have the capacity to colonize their host, degrade specific tissues, and circumvent the host immune system. to study this aspect of pathogenesis, we focused on three metalloproteases, inha1, inha2, and inha3, which share more than 66% identity. the expression of these metalloprotease genes was assessed by transcriptional fusions with a lacz reporter gene. the expression profiles suggest a complementary time course of inha production. indeed, ... | 2010 | 19837797 |
| rapid identification of bacterial isolates from aqueous kava (piper methysticum) extracts by polymerase chain reaction and dna sequencing. | fresh kava beverages have a limited shelf life under refrigerated conditions. the objective of this study was to isolate and identify bacteria in aqueous extracts of kava rhizome. | 2009 | 19843208 |
| confinement regulates complex biochemical networks: initiation of blood clotting by "diffusion acting". | this study shows that environmental confinement strongly affects the activation of nonlinear reaction networks, such as blood coagulation (clotting), by small quantities of activators. blood coagulation is sensitive to the local concentration of soluble activators, initiating only when the activators surpass a threshold concentration, and therefore is regulated by mass transport phenomena such as flow and diffusion. here, diffusion was limited by decreasing the size of microfluidic chambers, and ... | 2009 | 19843446 |
| characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines. | microbiologically influenced corrosion is a problem commonly encountered in facilities in the oil and gas industries. the present study describes bacterial enumeration and identification in diesel and naphtha pipelines located in the northwest and southwest region in india, using traditional cultivation technique and 16s rdna gene sequencing. phylogenetic analysis of 16s rrna sequences of the isolates was carried out, and the samples obtained from the diesel and naphtha-transporting pipelines sh ... | 2010 | 19844704 |
| antibacterial compounds from mushrooms ii: lanostane triterpenoids and an ergostane steroid with activity against bacillus cereus isolated from fomitopsis pinicola. | anti- bacillus cereus bioassay-guided fractionation of a crude extract of the american mushroom, fomitopsis pinicola, was performed using thin-layer chromatography, sephadex lh-20 column chromatography, and preparative-scale hplc. five lanostane triterpenoids (1-5) and one ergostane steroid (6) were isolated and identified. compound 1 is a new lanostane triterpenoid, and its structure was determined using 1d and 2d nmr experiments, hr-ms, and physical data. each of the purified compounds (1-6) w ... | 2010 | 19847745 |
| inactivation of food pathogen bacillus cereus by photosensitization in vitro and on the surface of packaging material. | the study was focused on the possibility to inactivate food pathogen bacillus cereus by 5-aminolevulinic acid (ala) - based photosensitization in vitro and after adhesion on the surface of packaging material. | 2009 | 19849812 |
| extremely long time stability study of selected antibiotic standards. | a study was conducted to investigate the quality of reference substances which were produced long before the introduction of advanced analysis and purification techniques. the quality and antimicrobial activity of who and ep oxytetracycline, doxycycline, colistin and spiramycin reference standards were assessed. the oldest substance was stored for 54 years in a freezer. assay and purity tests were conducted according to ph. eur. 6.0. additionally, antibacterial activity was tested with the micro ... | 2010 | 19850430 |
| fatal spontaneous bacterial peritonitis and necrotizing fasciitis with bacteraemia caused by bacillus cereus in a patient with cirrhosis. | we report a case of spontaneous bacterial peritonitis and necrotizing fasciitis caused by bacillus cereus in a cirrhotic patient without preceding disruption of skin or symptoms of gastroenteritis. this rapidly fatal infection due to b. cereus adds to the long list of aetiologies of infectious complications among patients with cirrhosis of the liver. | 2010 | 19850708 |
| identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks. | in this study, a novel avian beta-defensin (avbd) was isolated from duck pancreas. the complete nucleotide sequence of the gene contained an 195 bp open reading frame encoding 64 amino acids. homology, characterization and comparison of the gene with avbd from other avian species confirmed that it was duck avbd2. the mrna expression of the gene was analyzed in 17 tissues from 21-day-old ducks. avbd2 was highly expressed in the trachea, crop, heart, bone marrow, and pancreas; moderately expressed ... | 2009 | 19851734 |
| comparative analysis of transcriptional and physiological responses of bacillus cereus to organic and inorganic acid shocks. | comparative phenotype and transcriptome analyses were performed with bacillus cereus atcc 14579 exposed to ph 5.5 set with different acidulants including hydrochloric acid (hcl), lactic acid (hl) and acetic acid (hac). phenotypes observed included a decreased growth rate (with hcl), bacteriostatic and bactericidal conditions, with 2mm undissociated hac or hl, and 15mm undissociated hac, respectively. in the latter condition a concomitant decrease in intracellular atp levels was observed. the tra ... | 2010 | 19853945 |
| structure and regulation of the gab gene cluster, involved in the gamma-aminobutyric acid shunt, are controlled by a sigma54 factor in bacillus thuringiensis. | the structure and regulation of the gab gene cluster, involved in gamma-aminobutyric acid (gaba) shunt, were studied by characterizing gabt and gabd genes cloned from bacillus thuringiensis. deletions of the gabt and gabd genes in b. thuringiensis strain hd-73 did not affect the growth of mutant strains in rich culture media, but the growth of a gabt deletion mutant strain was reduced in basic media (containing 0.2% gaba). genome analysis indicates that the structure of the gab gene cluster in b ... | 2010 | 19854901 |
| sphingomyelinase-induced phase transformations: causing morphology switches and multiple-time-domain ceramide generation in model raft membranes. | sphingomyelinase (smase) has been shown to be involved in a variety of cell regulation processes by reorganizing the cell membrane morphology. here we report that smase can induce a reaction-induced and a solvent-mediated phase transformation, causing switches of three stationary membrane morphologies and multiple-time-domain ceramide generation in model raft membranes. the reaction-induced phase transformation, triggered by the addition of smase, transforms a pre-existing morphology to a long-l ... | 2010 | 19863058 |
| response of bacillus cereus atcc 14579 to challenges with sublethal concentrations of enterocin as-48. | enterocin as-48 is produced by enterococcus faecalis s48 to compete with other bacteria in their environment. due to its activity against various gram positive and some gram negative bacteria it has clear potential for use as a food preservative. here, we studied the effect of enterocin as-48 challenges on vegetative cells of bacillus cereus atcc 14579 by use of transcriptome analysis. | 2009 | 19863785 |
| chemical and morphological studies of bacterial spore formation : i. the formation of spores in bacillus cereus. | experimental conditions were developed whereby a culture of bacillus cereus formed spores with reasonable synchrony following a growth cycle of some 8 hours. the cytology of this metamorphosis was studied by dark phase contrast, bright-field microscopy and electron microscopy of thin sections. particular attention has been paid to the changes in chromatin patterns and these have been correlated with quantitative chemical estimations of the nucleic acids. the cell commencing sporulation contains ... | 1959 | 19866561 |
| the alkaline isopotential point of the bacterial cell. | irregularities in migration velocity of bacterial cells in the highly alkaline solutions are due to the buffering effect of the cells upon the immediately adjacent zone of menstruum. consistent results can be obtained by shaking the suspension thoroughly before placing it in the electrophoretic cell. when observed in this way both bacillus cereus and bacterium coli show an isopotential point near ph 13.5, that for bacillus cereus being slightly below, and that for bacterium coli slightly above t ... | 1924 | 19872109 |
| assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance. | to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health. | 2009 | 19880150 |
| detoxification and discoloration of moroccan olive mill wastewater by electrocoagulation. | the objective of the present study was to assess the electrocoagulation treatment of olive mill wastewater using an aluminum electrode. we have examined the effect of the following parameters on the removal of chemical oxygen demand (cod), polyphenols and dark color removal efficiency: electrolysis time, current density, chloride concentration and initial ph. the olive mill wastewater (omw)--diluted 5 times--used in this study had 20.000 mg/l chemical oxygen demand, 3.6 ms/cm conductivity and ac ... | 2010 | 19880250 |
| the silicon layer supports acid resistance of bacillus cereus spores. | silicon (si) is considered to be a "quasiessential" element for most living organisms. however, silicate uptake in bacteria and its physiological functions have remained obscure. we observed that si is deposited in a spore coat layer of nanometer-sized particles in bacillus cereus and that the si layer enhances acid resistance. the novel acid resistance of the spore mediated by si encapsulation was also observed in other bacillus strains, representing a general adaptation enhancing survival unde ... | 2010 | 19880606 |
| e. coli, p. aeruginosa, and b. cereus bacteria sterilization using afterglow of non-thermal plasma at atmospheric pressure. | we developed and employed a new geometrical structure of dielectric barrier discharge in atmospheric pressure for bacterial broad spectrum sterilization. we utilized a plasma source having an ac power supply at 50 hz and 5,400 v (rms value). we prepared suspensions of the gram-negative bacteria species (escherichia coli, pseudomonas aeruginosa) and a gram-positive of bacillus cereus with luria-bertani broth media up to od(600 nm) = 0.25 of mcfarland standard. afterglow of non-thermal atmospheric ... | 2010 | 19882114 |
| analysis of the involvement of chitin-binding domain of chicw in antifungal activity, and engineering a novel chimeric chitinase with high enzyme and antifungal activities. | an antifungal chitinase, chicw, produced by bacillus cereus 28-9 is effective against conidial germination of botrytis elliptica, the causal agent of lily leaf blight. chicw as a modular enzyme consists of a signal peptide, a catalytic domain, a fibronectin type-iii-like domain, and a chitin-binding domain. when two c-terminal domains of chicw were truncated, chicwdeltafc (lacking the chitin-binding domain and fibronectin type iii-like domain) lost its antifungal activity. since chicwdeltaac (la ... | 2009 | 19884776 |
| selective adhesion of bacillus cereus spores on heterogeneously wetted silicon nanowires. | the article reports on the selective adhesion of bacillus cereus spores on patterned and heterogeneously wetted superhydrophobic silicon nanowires surfaces. superhydrophilic patterns on superhydrophobic silicon nanowire (sinw) surfaces were prepared by a standard optical lithography technique. exposure of the patterned surface to a suspension of b. cereus spores in water led to their specific adsorption in superhydrophobic areas. comparable results were obtained on a patterned hydrophobic/hydrop ... | 2010 | 19891454 |
| enhancement of surfactin production of bacillus subtilis fmbr by replacement of the native promoter with the pspac promoter. | bacillus subtilis fmbr-1 was obtained from wild-type b. subtilis fmbr by replacement of the native promoter of the surfactin operon with the inducible promoter pspac. the recombinant b. subtilis fmbr-1 produced more antibacterial substances than the wild-type strain. the overproducing phenotype was related to the enhancement of antagonistic activities against bacillus cereus. hplc peaks of surfactin for recombinant fmbr-1 showed patterns of lipopeptides similar to those of the wild-type strain, ... | 2009 | 19898540 |
| transfer of bacillus cereus spores from packaging paper into food. | food packaging papers are not sterile, as the manufacturing is an open process, and the raw materials contain bacteria. we modeled the potential transfer of the bacillus cereus spores from packaging paper to food by using a green fluorescent protein-expressing construct of bacillus thuringiensis bt 407cry(-) [pht315omega(papha3-gfp)], abbreviated bt-1. paper (260 g m(-2)) containing bt-1 was manufactured with equipment that allowed fiber formation similar to that of full-scale manufactured paper ... | 2009 | 19903384 |
| survival during cooking and growth from spores of diarrheal and emetic types of bacillus cereus in rice. | bacillus cereus is a gram-positive, spore-forming, facultative anaerobe that is responsible for two types of gastrointestinal diseases: emesis and diarrhea. a significant difference in the d(95 degrees c)-values of spores of the emetic and the diarrheal types was initially determined. a mixture of b. cereus spores of the diarrheal type was inoculated into cooked rice. at inoculation levels of 2.5 x 10(2) spores per g of rice, cell numbers of 6.64 log were detected after 22 h at 20 degrees c and ... | 2009 | 19903405 |
| intramolecular amide bonds stabilize pili on the surface of bacilli. | gram-positive bacteria elaborate pili and do so without the participation of folding chaperones or disulfide bond catalysts. sortases, enzymes that cut pilin precursors, form covalent bonds that link pilin subunits and assemble pili on the bacterial surface. we determined the x-ray structure of bcpa, the major pilin subunit of bacillus cereus. the bcpa precursor encompasses 2 ig folds (cna(2) and cna(3)) and one jelly-roll domain (xna) each of which synthesizes a single intramolecular amide bond ... | 2009 | 19903875 |
| generation of thiocillin variants by prepeptide gene replacement and in vivo processing by bacillus cereus. | the thiocillins are natural-product antibiotics derived from ribosomally encoded peptides that undergo extensive posttranslational modifications to yield the mature trithiazolylpyridine-containing macrocyclic compound. poor pharmacokinetic properties have prevented the clinical use of these highly potent antibiotics. through in vivo manipulation of the gene responsible for production of the thiocillin precursor peptide, we have generated 65 novel thiocillin variants, allowing us to explore struc ... | 2009 | 19911780 |
| a downstream process for production of a viable and stable bacillus cereus aquaculture biological agent. | biological products offer advantages over chemotherapeutics in aquaculture. adoption in commercial application is lacking due to limitations in process and product development that address key end user product requirements such as cost, efficacy, shelf life and convenience. in previous studies, we have reported on the efficacy, physiological robustness and low-cost spore production of a bacillus cereus isolate (nrrl 100132). this study examines the development of suitable spore recovery, drying, ... | 2010 | 19921182 |
| new antibacterial and cytotoxic activities of falcarindiol isolated in crithmum maritimum l. leaf extract. | bioassay-guided fractionation of a chloroformic extract obtained from crithmum maritimum leaves led to the chemical isolation of falcarindiol, a polyacetylene widely distributed within the apiaceae family. structure of this compound was confirmed by nmr and activity was tracked using a screening microplate bioassay. falcarindiol strongly inhibited the growth of micrococcus luteus and bacillus cereus, with a minimum inhibitory concentration (mic) value of 50 microg ml(-1). moreover, this compound ... | 2010 | 19922760 |
| in vitro 5-phosphoribosyl 1-pyrophosphate-independent salvage biosynthesis of ribo- and deoxyriboadenine nucleotides in bacillus cereus. | the pools of free ribose 1-phosphate and deoxyribose 1-phosphate have been measured in bacillus cereus. it is shown that crude extracts of the same organism can actively utilize the sugar phosphates to convert adenine to atp and deoxyatp, via a 'salvage' pathway, involving adenine ribosylation (or deoxyribosylation), followed by multiple phosphorylation steps. the biosynthetic pathway operates even in the presence of excess p(i') thus showing that purine nucleoside phosphorylases may function in ... | 1985 | 19927404 |
| phytochemical profile and anticholinesterase and antimicrobial activities of supercritical versus conventional extracts of satureja montana. | winter savory satureja montana is a medicinal herb used in traditional gastronomy for seasoning meats and salads. this study reports a comparison between conventional (hydrodistillation, hd, and soxhlet extraction, se) and alternative (supercritical fluid extraction, sfe) extraction methods to assess the best option to obtain bioactive compounds. two different types of extracts were tested, the volatile (sfe-90 bar, second separator vs hd) and the nonvolatile fractions (sfe-250 bar, first and se ... | 2009 | 19928761 |
| biodegradation of polyacrylamide by bacteria isolated from activated sludge and oil-contaminated soil. | polyacrylamide (pam), a linear water soluble polymeric compound with high molecular weight, is extensively used for oil production in china. compared with the physico-chemical degradation of pam, there is no acrylamide monomer, which causes peripheral neuropathy, released in the process of biodegradation. unfortunately, few microorganisms have been isolated which can degrade pam. in this study, two pam-degrading bacterial strains, named hwbi and hwbii, were isolated from the activated sludge and ... | 2010 | 19932560 |
| bacillus thuringiensis serovars bolivia, vazensis and navarrensis meet the description of bacillus weihenstephanensis. | the bacillus cereus sensu lato group comprises six related species: b. cereus, b. anthracis, b. thuringiensis, b. mycoides, b. pseudomycoides and b. weihenstephanensis. bacillus thuringiensis is a mesophile. it is distinguished from other members of the b. cereus group by the apparition of an inclusion body upon sporulation. b. weihenstephanensis, however, is a psychrotolerant. it grows at 7 degrees c but not at 43 degrees c. it is further characterised by the presence of specific signature sequ ... | 2010 | 19937033 |
| discrimination and phylogenomic classification of bacillus anthracis-cereus-thuringiensis strains based on lc-ms/ms analysis of whole cell protein digests. | modern taxonomy, diagnostics, and forensics of bacteria benefit from technologies that provide data for genome-based classification and identification of strains; however, full genome sequencing is still costly, lengthy, and labor intensive. therefore, other methods are needed to estimate genomic relatedness among strains in an economical and timely manner. although dna-dna hybridization and techniques based on genome fingerprinting or sequencing selected genes like 16s rdna, gyrb, or rpob are f ... | 2010 | 19938824 |
| synthesis of nitroimidazole derived oxazolidinones as antibacterial agents. | a series of n-alkylated derivatives of nitroimidazolyl oxazolidinones 6a-i with various substituent at n-1 position of the nitroimidazole were synthesized and their in-vitro antibacterial activities were evaluated against several gram-positive and gram-negative bacteria. the 6a was found to be most potent compound in the series with mic at 0.097 microg/ml against bacillus cereus mtcc 430.). both 6a and 6f did not exhibit any toxicity towards mammalian cell l929. | 2010 | 19942324 |
| toxinogenic and spoilage potential of aerobic spore-formers isolated from raw milk. | the harmful effects on the quality and safety of dairy products caused by aerobic spore-forming isolates obtained from raw milk were characterized. quantitative assessment showed strains of bacillus subtilis, the bacillus cereus group, paenibacillus polymyxa and bacillus amyloliquefaciens to be strongly proteolytic, along with bacillus licheniformis, bacillus pumilus and lysinibacillus fusiformis to a lesser extent. lipolytic activity could be demonstrated in strains of b. subtilis, b. pumilus a ... | 2010 | 19944473 |
| detection of bacillus cereus with enteropathogenic potential by multiplex real-time pcr based on sybr green i. | in order to meet the growing demand for fast and reliable detection of potentially toxinogenic bacillus cereus, we developed a multiplex real-time pcr assay based on sybr green i with subsequent melting curve analysis. we designed and selected primers specific for genes of toxins responsible for diarrhoea (nhea, hbld and cytk1) and emesis (ces). a panel of 337 bacillus strains was applied to the novel method on light cycler 2.0 with average melting temperature (t(m)) values of 73.85 degrees c (n ... | 2010 | 19944752 |
| fnr mediates carbohydrate-dependent regulation of catabolic and enterotoxin genes in bacillus cereus f4430/73. | we had previously demonstrated that fnr is required for fermentative growth and oxic production of hemolysin bl (hbl) and non-hemolytic enterotoxin (nhe) in the food-borne pathogen bacillus cereus f4430/73. in the present work, the regulatory impact of fnr on microaerobic growth and enterotoxin production in response to carbohydrates was examined using glucose, fructose, sucrose or a glucose-fructose mixture as carbon and energy sources. growth parameters, byproduct spectra and transcription lev ... | 2010 | 19944753 |
| isolation and characterization of hyper phenol tolerant bacillus sp. from oil refinery and exploration sites. | bacillus cereus mtcc 9817 strain akg1 and b. cereus mtcc 9818 strain akg2 were isolated from petroleum refinery and oil exploration site, respectively. the 16s rdna sequence of strain akg1 showed the closest relation to b. cereus 99.63% and bacillus coagulans 99.63% followed by 99.34% homology with bacillus thuringiensis strain 2pr56-10. akg2 is mostly related to b. thuringiensis strain cmg 861 with 99.37% homology. the similarity search between akg1 and akg2 gave the lowest similarity 99.19% am ... | 2010 | 19945784 |
| synergistic effects of the lactobacillus acidophilus surface layer and nisin on bacterial growth. | we have previously described a murein hydrolase activity for the surface layer (s-layer) of lactobacillus acidophilus atcc 4356. here we show that, in combination with nisin, this s-layer acts synergistically to inhibit the growth of pathogenic gram-negative salmonella enterica and potential pathogenic gram-positive bacteria, staphylococcus aureus and bacillus cereus. in addition, bacteriolytic effects were observed for the gram-positive species tested. we postulate that the s-layer enhances the ... | 2010 | 19948852 |
| application of the bactericidal activity of epsilon-poly-l-lysine to the storage of human platelet concentrates. | epsilon-poly-l-lysine (epsilon-pll) is a polypeptide comprising approximately 30 l-lysine subunits generated by bond formation between alpha-carboxy and epsilon-amino groups. it is an approved antimicrobial food preservative in japan. however, the efficacy of epsilon-pll as an antibacterial additive for storage of human platelet concentrates (pcs) is not known. | 2010 | 19951313 |
| examination of genome homogeneity in prokaryotes using genomic signatures. | dna word frequencies, normalized for genomic at content, are remarkably stable within prokaryotic genomes and are therefore said to reflect a "genomic signature." the genomic signatures can be used to phylogenetically classify organisms from arbitrary sampled dna. genomic signatures can also be used to search for horizontally transferred dna or dna regions subjected to special selection forces. thus, the stability of the genomic signature can be used as a measure of genomic homogeneity. the fact ... | 2009 | 19956556 |
| a genome-wide survey for host response of silkworm, bombyx mori during pathogen bacillus bombyseptieus infection. | host-pathogen interactions are complex relationships, and a central challenge is to reveal the interactions between pathogens and their hosts. bacillus bombysepticus (bb) which can produces spores and parasporal crystals was firstly separated from the corpses of the infected silkworms (bombyx mori). bb naturally infects the silkworm can cause an acute fuliginosa septicaemia and kill the silkworm larvae generally within one day in the hot and humid season. bb pathogen of the silkworm can be used ... | 2009 | 19956592 |
| ilsa, a unique surface protein of bacillus cereus required for iron acquisition from heme, hemoglobin and ferritin. | the human opportunistic pathogen bacillus cereus belongs to the b. cereus group that includes bacteria with a broad host spectrum. the ability of these bacteria to colonize diverse hosts is reliant on the presence of adaptation factors. previously, an ivet strategy led to the identification of a novel b. cereus protein (ilsa, iron-regulated leucine rich surface protein), which is specifically expressed in the insect host or under iron restrictive conditions in vitro. here, we show that ilsa is l ... | 2009 | 19956654 |
| a novel hybrid kinase is essential for regulating the sigma(b)-mediated stress response of bacillus cereus. | a common bacterial strategy for monitoring environmental challenges is to use two-component systems, which consist of a sensor histidine kinase (hk) and a response regulator (rr). in the food-borne pathogen bacillus cereus, the alternative sigma factor sigma(b) is activated by the rr rsby. here we present strong indications that the pp2c-type phosphatase rsby receives its input from the multi-sensor hybrid kinase bc1008 (renamed rsbk). genome analyses revealed that, across bacilli, rsby and rsbk ... | 2010 | 19958380 |
| extraction and characterization of chitin, chitosan, and protein hydrolysates prepared from shrimp waste by treatment with crude protease from bacillus cereus sv1. | chitin is a polysaccharide found in abundance in the shell of crustaceans. in this study, the protease from bacillus cereus sv1 was applied for chitin extraction from shrimp waste material of metapenaeus monoceros. a high level of deproteinization 88.8% +/- 0.4 was recorded with an e/s ratio of 20. the demineralization was completely achieved within 6 h at room temperature in hcl 1.25 m, and the residual content of calcium in chitin was below 0.01%. (13)c cp/mas-nmr spectral analysis of chitin p ... | 2010 | 19960271 |
| antimicrobial properties of the stem bark of saraca indica (caesalpiniaceae). | chloroform, methanol, aqueous and ethanolic extracts of the stem bark of saraca indica were investigated for their antibacterial and antifungal activity against standard strains of staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, bacillus cereus, klebsiella pneumoniae, proteus mirabilis, salmonella typhimurium and streptococcus pneumoniae and the fungi: candida albicans and cryptococcus albidus. methanolic and aqueous extract exhibited antimicrobial activity with mic ranging from ... | 2009 | 19961043 |
| hydrolysis of amylopectin by amylolytic enzymes: level of inner chain attack as an important analytical differentiation criterion. | differences in amylase action pattern on amylopectin were demonstrated by the relation between the decrease in potassium iodide-iodine binding of waxy maize starch and the increase in reducing value during hydrolysis, as expressed by the rv(80) value (i.e., the reducing value for a potassium iodide-iodine binding value of 80% of that of the starting material). in the initial stages of the hydrolysis, the ratio of the increase in the level of reducing polysaccharides to the increase in the total ... | 2010 | 19962134 |
| development of a stable isotope dilution analysis for the quantification of the bacillus cereus toxin cereulide in foods. | an increasing number of severe food borne intoxications are caused by a highly stable depsipeptide, named cereulide, which is produced by emetic bacillus cereus strains. as cereulide poses a health risk to humans, the development of an appropriate method for the analysis of this toxin is mandatory. therefore, the reference material of cereulide as well as its (13)c(6)-isotopologue was prepared by means of a biosynthetic approach using a b. cereus culture, followed by a rapid but efficient downst ... | 2010 | 19994891 |
| a pilot study of the microbiological quality of culturally diverse, ready-to-eat foods from selected retail establishments in melbourne, australia. | in recent years, there has been an increasing number of foodborne outbreaks linked to the consumption of culturally diverse foods. this appears to be because of the increasing quantity of culturally diverse foods available and a preference to store these foods, some of which are considered potentially hazardous, at ambient temperature. this practice may contravene temperature requirements defined by the food standards code. a lack of understanding of the hazardous nature of some culturally prepa ... | 2010 | 20001329 |
| soya bean tempe extracts show antibacterial activity against bacillus cereus cells and spores. | tempe, a rhizopus ssp.-fermented soya bean food product, was investigated for bacteriostatic and/or bactericidal effects against cells and spores of the food-borne pathogen bacillus cereus. | 2010 | 20002864 |
| multi-tools approach for food safety risk management of steam meals. | steamed meals comprise a new type of meal in which various raw ingredients are packed together and then cooked by the consumer just before consumption. the presence of raw ingredients and the absence of any inactivation step before home cooking could significantly impact the safety of these meals. in this study some of the many tools available to assess food safety were combined to determine the factors affecting the food safety of this new kind of meal. first the hazards were identified using s ... | 2009 | 20003753 |
| arbuscular mycorrhizal fungi, bacillus cereus, and candida parapsilosis from a multicontaminated soil alleviate metal toxicity in plants. | we investigated if the limited development of trifolium repens growing in a heavy metal (hm) multicontaminated soil was increased by selected native microorganisms, bacteria (bacillus cereus (bc)), yeast (candida parapsilosis (cp)), or arbuscular mycorrhizal fungi (amf), used either as single or dual inoculants. these microbial inoculants were assayed to ascertain whether the selection of hm-tolerant microorganisms can benefit plant growth and nutrient uptake and depress hm acquisition. the inoc ... | 2010 | 20013261 |
| primary multiple cerebral invasion in acute promyelocytic leukemia dramatically worsened by all-trans retinoic acid. | 2010 | 20015546 | |
| direct-imaging-based quantification of bacillus cereus atcc 14579 population heterogeneity at a low incubation temperature. | bacillus cereus atcc 14579 was cultured in microcolonies on anopore strips near its minimum growth temperature to directly image and quantify its population heterogeneity at an abusive refrigeration temperature. eleven percent of the microcolonies failed to grow during low-temperature incubation, and this cold-induced population heterogeneity could be partly attributed to the loss of membrane integrity of individual cells. | 2010 | 20023091 |
| physical characteristics of spores of food-associated isolates of the bacillus cereus group. | all 47 food-borne isolates of bacillus cereus sensu stricto, as well as 10 of 12 food-borne, enterotoxigenic isolates of bacillus thuringiensis, possessed appendages. spores were moderately to highly hydrophobic, and each had a net negative charge. these characteristics indicate that spores of food-associated b. thuringiensis and not only b. cereus sensu stricto have high potential to adhere to inert surfaces. | 2010 | 20023097 |
| characterisation and toxicity of bacillus thuringiensis strains from hazelnut pests and fields. | in order to find and identify more toxic insecticidal bacillus thuringiensis berliner (bt) strains, a survey was carried out of b. thuringiensis isolate pests belonging to coleoptera, lepidoptera and diptera and from soils in hazelnut fields. of 16 isolates having bacillus cereus-b. thuringiensis morphology, eight were classified as b. thuringiensis because of the production of parasporal delta-endotoxin crystals. | 2010 | 20024949 |
| involvement of motility and flagella in bacillus cereus biofilm formation. | bacillus cereus is a food-borne pathogen and a frequent contaminant of food production plants. the persistence of this pathogen in various environments results from the formation of spores and of biofilms. to investigate the role of the b. cereus flagellar apparatus in biofilm formation, we constructed a non-flagellated mutant and a flagellated but non-motile mutant. unexpectedly, we found that the presence of flagella decreased the adhesion of the bacterium to glass surfaces. we hypothesize tha ... | 2010 | 20035003 |
| dissecting interactions between nucleosides and germination receptors in bacillus cereus 569 spores. | bacillus cereus 569 spores germinate either with inosine as a sole germinant or with a combination of nucleosides and l-alanine. whereas the inosine-only germination pathway requires the presence of two different germination receptors (geri and gerq) to be activated, the nucleoside/alanine germination pathway only needs one of the two receptors. to differentiate how nucleoside recognition varies between the inosine-only germination pathway and the nucleoside/alanine germination pathway, we teste ... | 2010 | 20035009 |
| occurrence of psychrotolerant bacillus cereus group strains in ice creams. | the occurrences of bacillus cereus group strains in 40 ice cream samples were investigated. among 109 isolated b. cereus group strains confirmed by 16s rdna sequence analysis only 50 were identified as b. cereus and one as b. thuringiensis by using fda (u.s. food and drug administration) standard, indicating the two identification standards were highly inconsistent. furthermore, the psychrotolerant growth properties and the occurrence of specific psychrotolerant genes of the isolates were also s ... | 2010 | 20036024 |
| photodynamic antimicrobial activity of avian eggshell pigments. | pigmentation in avian eggshells appears to be associated with shell strength, temperature regulation, and camouflage. the pigments found in eggshells are mainly porphyrins, which have been utilized therapeutically as photosensitizers. here, we examined the photoinactivation of gram-positive (staphylococcus aureus, bacillus cereus) and gram-negative bacteria (escherichia coli, salmonella enteritidis) by hen eggshells and their pigments. the results indicated that eggshells have a light-dependent ... | 2010 | 20036664 |
| identification of the main promoter directing cereulide biosynthesis in emetic bacillus cereus and its application for real-time monitoring of ces gene expression in foods. | cereulide, the emetic bacillus cereus toxin, is synthesized by cereulide synthetase via a nonribosomal peptide synthetase (nrps) mechanism. previous studies focused on the identification, structural organization, and biochemical characterization of the ces gene locus encoding cereulide synthetase; however, detailed information about the transcriptional organization of the ces genes was lacking. the present study shows that the cesptabcd genes are transcribed as a 23-kb polycistronic transcript, ... | 2010 | 20038713 |
| identification of bacillus cereus in a chungkukjang that showed high anticancer effects against ags human gastric adenocarcinoma cells. | anticancer effects of chungkukjang (a korean short-term fermented soy paste) were studied in human gastric adenocarcinoma cells, and bacillus strains from chungkukjang were isolated and identified. before the experiments, six different chungkukjang products (k-, m-, mn-, o-, os-, and h-chungkukjangs) were purchased from a folk village in the sunchang region, jeonbuk, republic of korea. based on sensory evaluation tests and general chemical and quality studies, k-, h-, and m-chungkukjangs were se ... | 2009 | 20041781 |
| enhancing atp-based bacteria and biofilm detection by enzymatic pyrophosphate regeneration. | the manufacturing processes of many electronic and medical products demand the use of high-quality water. hence the water supply systems for these processes are required to be examined regularly for the presence of microorganisms and microbial biofilms. among commonly used bacteria detection approaches, the atp luminescence assay is a rapid, sensitive, and easy to perform method. the aim of this study is to investigate whether atp regeneration from inorganic pyrophosphate, a product of the atp l ... | 2010 | 20043864 |
| superdormant spores of bacillus species germinate normally with high pressure, peptidoglycan fragments, and bryostatin. | superdormant spores of bacillus cereus and bacillus subtilis germinated just as well as dormant spores with pressures of 150 or 500 mpa and with or without heat activation. superdormant b. subtilis spores also germinated as well as dormant spores with peptidoglycan fragments or bryostatin, a ser/thr protein kinase activator. | 2010 | 20047906 |
| analysis of the antimicrobial activity of propolis and lysozyme in semisolid emulsion systems. | propolis as an active natural substance is attractive due to its antimicrobial and antimycotic properties. lysozyme was added to semisolid dermatological preparations as a complementary substance capable of potentiating their antimicrobial and antimycotic effect; this substance has been used for several decades as a preservative in food industry. the aim of this study was to model a semisolid emulsion system (o/w) for cutaneous use with moisturizing and antimicrobial properties, where the active ... | 2009 | 20050532 |
| production, characterization, and antimicrobial activity of a bacteriocin from newly isolated enterococcus faecium ij-31. | this work aimed to isolate and characterize enterococcus spp. from indigenous dairy products in islamabad, pakistan. by classical microbiological techniques, one strain from a butter sample was identified to be enterococcus faecium, and we designated it e. faecium ij-31. the precise identity of this strain was then established by determining the sequence of its 16s and 23s rrna genes. the sequence homology searches revealed matches with a number of previously reported strains, such as e. faecium ... | 2010 | 20051203 |
| multi-functional p(3hb) microsphere/45s5 bioglass-based composite scaffolds for bone tissue engineering. | novel multi-functional p(3hb) microsphere/45s5 bioglass-based composite scaffolds exhibiting potential for drug delivery were developed for bone tissue engineering. 45s5 bioglass-based glass-ceramic scaffolds of high interconnected porosity produced using the foam-replication technique were coated with biodegradable microspheres (size<2 microm) made from poly(3-hydroxybutyrate), p(3hb), produced using bacillus cereus spv. a solid-in-oil-in-water emulsion solvent extraction/evaporation technique ... | 2010 | 20056174 |
| analysis of acid-stressed bacillus cereus reveals a major oxidative response and inactivation-associated radical formation. | acid stress resistance of the food-borne human pathogen bacillus cereus may contribute to its survival in acidic environments, such as encountered in soil, food and the human gastrointestinal tract. the acid stress responses of b. cereus strains atcc 14579 and atcc 10987 were analysed in aerobically grown cultures acidified to ph values ranging from ph 5.4 to ph 4.4 with hcl. comparative phenotype and transcriptome analyses revealed three acid stress-induced responses in this ph range: growth ra ... | 2010 | 20074238 |
| comparison of two optical-density-based methods and a plate count method for estimation of growth parameters of bacillus cereus. | quantitative microbiological models predicting proliferation of microorganisms relevant for food safety and/or food stability are useful tools to limit the need for generation of biological data through challenge testing and shelf-life testing. the use of these models requires quick and reliable methods for the generation of growth data and estimation of growth parameters. growth parameter estimation can be achieved using methods based on plate counting and methods based on measuring the optical ... | 2010 | 20081006 |
| isolation, structure elucidation and biological activity of angucycline antibiotics from an epiphytic yew streptomycete. | in the course of study of epiphytic microorganisms occurring on the surface of roots of taxus baccata l. a new strain streptomyces sp. ac113 was isolated. according to 16s ribosomal dna-based identification the new strain is 99% identical with streptomyces flavidofuscus. this strain cultivated in an arginine glycerol medium produced three major metabolites identified as (-)-8-o -methyltetrangomycin (1), 8-o -methyltetrangulol (2) and 8-o -methyl-7-deoxo-7-hydroxytetrangomycin (3). the chemical s ... | 2010 | 20082376 |
| safety assessment of recalled haemophilus influenzae type b (hib) conjugate vaccines--united states, 2007-2008. | on 13 december 2007, merck & co., inc. voluntarily recalled 1.2 million doses of haemophilus influenzae type b (hib) vaccines that had been distributed since april 2007 for concerns regarding potential bacillus cereus contamination. enhanced postrecall surveillance was conducted to detect vaccine-associated b. cereus infections. | 2010 | 20084617 |
| profile of bacteria and fungi on money coins. | to determine the quantity and quality of bacterial and fungi on money coins and to identify those that could pose a public health risk. | 2009 | 20084997 |
| antibacterial and antioxidant activities of the essential oil of artemisia echegarayi hieron. (asteraceae). | artemisia echegarayi hieron. (asteraceae) is commonly known in argentina as "ajenjo". many studies report high efficacy of essential oils against food-borne pathogenic bacteria. the antimicrobial activity and minimal inhibitory concentration of a. echegarayi essential oil were evaluated against seven bacterial species of significant importance in food hygiene, by using the disc diffusion assay and the micro-well dilution method, respectively. volatile components of the extract were analyzed by g ... | 2009 | 20085186 |
| single chain variable fragment antibodies selected by phage display against the sporozoite surface antigen s16 of cryptosporidium parvum. | two human single chain variable fragment (scfv) libraries were used to select clones that bound to the surface glycoprotein s16 of cryptosporidium parvum. panning of the tomlinson libraries i and j resulted in the isolation of nine distinct clones. of the four clones which had full-length scfv, three contained stop codons. the remaining five clones were truncated, with four missing the heavy chain, and one missing most of the light chain. the full-length clones exhibited better binding to native ... | 2010 | 20093113 |
| use of fatty acid methyl ester profiles for discrimination of bacillus cereus t-strain spores grown on different media. | the goal of this study was to determine if cellular fatty acid methyl ester (fame) profiling could be used to distinguish among spore samples from a single species (bacillus cereus t strain) that were prepared on 10 different medium formulations. to analyze profile differences and identify fame biomarkers diagnostic for the chemical constituents in each sporulation medium, a variety of statistical techniques were used, including nonmetric multidimensional scaling (nmds), analysis of similarities ... | 2010 | 20097814 |
| characterization of wet-heat inactivation of single spores of bacillus species by dual-trap raman spectroscopy and elastic light scattering. | dual-trap laser tweezers raman spectroscopy (ltrs) and elastic light scattering (els) were used to investigate dynamic processes during high-temperature treatment of individual spores of bacillus cereus, bacillus megaterium, and bacillus subtilis in water. major conclusions from these studies included the following. (i) after spores of all three species were added to water at 80 to 90 degrees c, the level of the 1:1 complex of ca(2+) and dipicolinic acid (cadpa; approximately 25% of the dry weig ... | 2010 | 20097820 |
| growth of microorganisms in total parenteral nutrition solutions without lipid. | to identify the microorganisms that can grow rapidly in total parenteral nutrition (tpn) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (staphylococcus aureus, serratia marcescens, bacillus cereus, and candida albicans) in tpn solutions without lipid. | 2010 | 20107529 |
| designed low amphipathic peptides with alpha-helical propensity exhibiting antimicrobial activity via a lipid domain formation mechanism. | although several low amphipathic peptides have been known to exhibit antimicrobial activity, their mode of action has not been completely elucidated. in this study, using designed low amphipathic peptides that retain different alpha-helical content and hydrophobicity, we attempted to investigate the mechanism of these properties. calorimetric and thermodynamic analyses demonstrated that the peptides induce formation of two lipid domains in an anionic liposome at a high peptide-to-lipid ratio. on ... | 2010 | 20109510 |
| isolation and characterization of an acrylamide-degrading bacillus cereus. | several local acrylamide-degrading bacteria have been isolated. one of the isolate that exhibited the highest growth on acrylamide as a nitrogen source was then further characterized. the isolate was tentatively identified as bacillus cereus strain dry135 based on carbon utilization profiles using biolog gp plates and partial 16s rdna molecular phylogeny. the isolate grew optimally in between the temperatures of 25 and 30 degrees c and within the ph range of 6.8 to 7.0. glucose, fructose, lactos ... | 2009 | 20112864 |
| antibacterial effects of minerals from ores indigenous to korea. | we tested the antibacterial properties of a mix of minerals consisting mainly of sericite, talc, and halloysite from korea. the preparation showed clear growth inhibition of the gram-negative bacteria escherichia coli and pseudomonas aeruginosa, and the gram-positive bacteria staphylococcus aureus subsp. aureus, s. epidermidis, and bacillus cereus, as well as the anaerobic bacterium propionibacterium acnes. these results indicate that this preparation, made from ore minerals indigenous to korea, ... | 2009 | 20112878 |
| prevalence of bacillus anthracis-like organisms and bacteriophages in the intestinal tract of the earthworm eisenia fetida. | stable infection of bacillus anthracis laboratory strains with environmental bacteriophages confers survival phenotypes in soil and earthworm intestinal niches (r. schuch and v. a. fischetti, plos one 4:e6532, 2009). here, the natural occurrence of two such b. anthracis-infective bacteriophages, wip1 and wip4, was examined in the intestines of eisenia fetida earthworms as part of a 6-year longitudinal study at a pennsylvania forest site. the wip1 tectivirus was initially dominant before being su ... | 2010 | 20118353 |
| chemistry, antioxidant, antibacterial and antifungal activities of volatile oils and their components. | the present paper reports the chemical composition, antioxidant and antibacterial activities of several essential oils and their components. analysis showed that three oils (carum carvi l., verbena officinalis l. and majorana hortensis l.) contained predominantly oxygenated monoterpenes, while others studied (pimpinella anisum l., foeniculum vulgare mill.) mainly contained anethole. c. carvi, v. officinalis and m. hortensis oils exhibited the most potent antioxidant activity, due their contents ... | 2009 | 20120118 |
| occurrence of antibiotic resistant bacteria in waste site of ede south west nigeria. | analysis of antibiotic resistant bacteria from composted waste site soil samples for two seasons were done, using prepared diagnostic test agar and direct sensitivity tests. all major isolated bacteria were subjected to various antibiotics. concurrent positive and negative tests were simultaneously studied. results show relatively high resistant and sensitive bacteria in both seasons. in wet seasons, the bacillus cereus concentrations are considerably reduced than in the dry. in the dry seasons, ... | 2009 | 20121016 |
| inha1, npra, and hlyii as candidates for markers to differentiate pathogenic from nonpathogenic bacillus cereus strains. | bacillus cereus is found in food, soil, and plants, and the ability to cause food-borne diseases and opportunistic infection presumably varies among strains. therefore, measuring harmful toxin production, in addition to the detection of the bacterium itself, may be key for food and hospital safety purposes. all previous studies have focused on the main known virulence factors, cereulide, hbl, nhe, and cytk. we examined whether other virulence factors may be specific to pathogenic strains. inha1, ... | 2010 | 20129969 |
| prevalence of emetic bacillus cereus in different ice creams in bavaria. | in this study, 809 samples of ice cream from different sources were investigated by using cultural methods for the presence of presumptive bacillus cereus. isolates from culture-positive samples were examined with a real-time pcr assay targeting a region of the cereulide synthetase gene (ces) that is highly specific for emetic b. cereus strains. the samples were collected from ice cream parlors and restaurants that produced their own ice cream and from international commercial ice cream companie ... | 2010 | 20132691 |
| antimicrobial activities of tapioca starch/decolorized hsian-tsao leaf gum coatings containing green tea extracts in fruit-based salads, romaine hearts and pork slices. | the antimicrobial activities of edible coatings based on a tapioca starch/decolorized hsian-tsao leaf gum (dhg) matrix with various green tea extracts (gtes) were evaluated. its effect on the shelf-life extension of fruit-based salads, romaine hearts, and pork slices were investigated as well. three types of gtes from hot water (80 degrees c) (w), 40% (e4) and 80% (e8) ethanol were prepared. it was found that all gtes showed pronounced inhibition on gram positive bacteria in agar media, includin ... | 2010 | 20138382 |
| effect of high pressure on the microbiological quality of cooked chicken during storage at normal and abuse refrigeration temperatures. | vacuum-packaged cooked poultry meat was treated at a range of pressures (400-600 mpa) and hold times (1, 2 and 10 min), followed by storage at 4 degrees , 8 degrees or 12 degrees c for up to 35 days. weissella viridescens was found to be the dominant microorganism in the pressure-treated meat, constituting 100% of the microflora identified at 500 and 600 mpa. none of the pressure-treated samples had obvious signs of spoilage during the 35 day storage period, even when the weissella count was >7 ... | 2010 | 20141945 |
| antibacterial activity of carvacrol and 2-nitro-1-propanol against single and mixed populations of foodborne pathogenic bacteria in corn flour dough. | cereal doughs are an important part of human diet, but at the same time can act as vehicles for the transmission of human pathogenic bacteria. in the present study, four pathogenic or toxinogenic bacteria (escherichia coli o157:h7, salmonella enterica serovar enteritidis, bacillus cereus and staphylococcus aureus) were inoculated in a dough made from corn flour in combination with the single antimicrobial compounds carvacrol and 2-nitro-1-propanol (2npoh). survival of single and mixed population ... | 2010 | 20141946 |
| germinant receptor diversity and germination responses of four strains of the bacillus cereus group. | four strains of the bacillus cereus group were compared for their germinant receptor composition and spore germination capacity. phylogenetic analysis of the germinant receptor encoding operons of the enterotoxic strains b. cereus atcc 14579 and atcc 10987, the emetic strain ah187, and the psychrotolerant strain bacillus weihenstephanensis kbab4, indicated a core group of five germinant receptor operons to be present in the four strains, with each strain containing one to three additional recept ... | 2010 | 20153067 |
| coupling of the hydrogen and polyhydroxyalkanoates (pha) production through anaerobic digestion from taihu blue algae. | coupling bio-production of hydrogen and polyhydroxyalkanoates (pha) from taihu blue algae through metabolites circulation was investigated. it was found that the ph adjustment, especially basification was more practical and efficient than other methods for the pretreatment of blue algae before anaerobic digestion. on this occasion, scod, biogas accumulation and hydrogen content reached 26 mg/gts, 500 ml and 37.2%, and which were 4.3, 1.3 and 14.4 times of those of the control group, respectively ... | 2010 | 20153165 |
| the swaposin-like domain of potato aspartic protease (stasp-psi) exerts antimicrobial activity on plant and human pathogens. | plant-specific insert domain (psi) is a region of approximately 100 amino acid residues present in most plant aspartic protease (ap) precursors. psi is not a true saposin domain; it is the exchange of the n- and c-terminal portions of the saposin like domain. hence, psi is called a swaposin domain. here, we report the cloned, heterologous expression and purification of psi from stasp 1 (solanum tuberosum aspartic protease 1), called stasp-psi. results obtained here show that stasp-psi is able to ... | 2010 | 20153392 |
| cytokine profile in a premature infant with systemic bacillus cereus infection. | 2010 | 20158644 | |
| role of the gerp operon in germination and outgrowth of bacillus anthracis spores. | germination of bacillus anthracis spores occurs when nutrients such as amino acids or purine nucleosides stimulate specific germinant receptors located in the spore inner membrane. the gerp(abcdef) operon has been suggested to play a role in facilitating the interaction between germinants and their receptors in spores of bacillus subtilis and bacillus cereus. b. anthracis mutants containing deletions in each of the six genes belonging to the orthologue of the gerp(abcdef) operon, or deletion of ... | 2010 | 20161744 |
| antibacterial activities of the extracts and conessine from holarrhena floribunda g. don. (apocynaceae). | the methanolic extract and conessine isolated from the stem bark of holarrhena floribunda (hf) were tested for their antibacterial activities on bacillus: bacillus cereus, bacillus subtilis, bacillus megaterium and bacillus stearothermophilus using the disc diffusion method. phytochemical analysis of the crude extract and fractions was also conducted. the inhibition parameters of the crude methanol extract and the total alkaloid fraction were determined using the macrodilution method. the result ... | 2007 | 20161899 |