Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| application of an automated immunomagnetic separation-enzyme immunoassay for the detection of salmonella enterica subspecies enterica from poultry environmental swabs. | an automated immunomagnetic separation (ims) and enzyme immunoassay (eia) was applied to the detection of salmonella enterica subspecies enterica serotypes from poultry environmental samples. the analytical sensitivity and specificity of the ims-eia for 46 s. enterica serotypes and 33 non-salmonellae isolates belonging to 21 different genera were 91.3% and 90.9%, respectively. from post enrichment s. enterica cultures, the limit of detection of the assay was estimated at 10(4)-10(6) cfu/ml. appl ... | 2009 | 19616586 |
| fatty acid profiling and proteomic analysis of salmonella enterica serotype typhimurium inactivated with supercritical carbon dioxide. | non-thermal sterilization and microbial inactivation processes are currently receiving much attention in food and pharmaceutical industries. in particular, since supercritical carbon dioxide (sc-co2) treatment, which is conducted at relatively low temperatures, is considered to be a promising alternative method to replace thermal sterilization processes that cannot be safely used in foods and bioactive materials. although sc-co2 has been applied to many microorganisms, the inactivation of microb ... | 2009 | 19616865 |
| characterization of salmonella enterica serotype typhimurium from outpatients of 28 hospitals in henan province in 2006. | to characterize the diarrheal patients with salmonella typhimurium (s. typhimurium) infections and to set up the first baseline for s. typhimurium pulsed-field gel electrophoresis (pfge) patterns in henan province, thus laying a foundation for comprehensive surveillance of salmonella in human as well as foods. | 2009 | 19618691 |
| role of nod1 in mucosal dendritic cells during salmonella pathogenicity island 1-independent salmonella enterica serovar typhimurium infection. | recent advances in immunology have highlighted the critical function of pattern-recognition molecules (prms) in generating the innate immune response to effectively target pathogens. nod1 and nod2 are intracellular prms that detect peptidoglycan motifs from the cell walls of bacteria once they gain access to the cytosol. salmonella enterica serovar typhimurium is an enteric intracellular pathogen that causes a severe disease in the mouse model. this pathogen resides within vacuoles inside the ce ... | 2009 | 19620349 |
| combining capillary electrophoresis with laser-induced fluorescence detection for the analysis of escherichia coli lysates. | with the goal of improving water quality control, in this study we combined ce-lif for the analysis of escherichia coli lysates under discontinuous conditions. prior to injecting a large-volume protein sample, a plug of 0.2% w/v sds was injected into the capillary filled with 2.0 m tris-borate (tb) solution (ph 10.3). during the courses of the process stacking, proteins migrated against the electroosmotic flow (eof) and entered a 1.7% w/v poly(ethylene oxide) solution that has been prepared in 2 ... | 2009 | 19621366 |
| [construction of a dual-promoter expression plasmid delivered by salmonella choleraesuis c500]. | salmonella choleraesuis c500 strain is an attenuated vaccine preventing piglet from paratyphoid and can also be used as a live vector of other dna vaccines. through mucosal immunization, immune response to specific antigens carried by it can be induced. to enhance the immune efficiency of dna vaccine it carried, promoter ptrc was inserted into the down stream of the human cytomegalovirus (cmv) immediate early promoter of eukaryotic expression plasmid pegfp-c1. then transcription terminator rrnbt ... | 2009 | 19621572 |
| [construction of attenuated salmonella choleraesuis vaccine strain expressing recombinant antigen of shiga-like toxin escherichia coli]. | to construct an attenuated salmonella choleraesuis vaccine strain expressing the gene slt-iieb and fedf of shiga-like toxin escherichia coli (sltec) o138 with balanced lethal system. | 2009 | 19621641 |
| improvement of an acid phosphatase/dhap-dependent aldolase cascade reaction by using directed evolution. | to enhance the phosphorylating activity of the bacterial nonspecific acid phosphatase from salmonella enterica ser. typhimurium lt2 towards dihydroxyacetone (dha), a mutant library was generated from the native enzyme. three different variants that showed enhanced activity were identified after one round of eppcr. the single mutant v78l was the most active and showed an increase in the maximal dhap concentration to 25 % higher than that of the wild-type enzyme at ph 6.0. this variant is 17 times ... | 2009 | 19623595 |
| meeting on establishment of consortium to study invasive salmonelloses in sub-saharan africa. | 2009 | 19624911 | |
| role of salmonella enterica lipopolysaccharide in activation of dendritic cell functions and bacterial containment. | in contrast to nonpathogenic bacteria, the gram-negative pathogen salmonella enterica is not eradicated, but persists in murine dendritic cells (dc). the molecular basis of this phenotype is unknown. we set out to characterize bacterial and dc functions that are involved in salmonella persistence. our data prove that neither bacterial nor host cell de novo protein biosynthesis is required for salmonella persistence in dc. we identified the salmonella o-ag of the lps of salmonella as an important ... | 2009 | 19625639 |
| molecular leakage at implant-abutment connection--in vitro investigation of tightness of internal conical implant-abutment connections against endotoxin penetration. | microleakage has been discussed as a major contributing factor for inflammatory reactions at the implant-abutment connection. in previous studies, the tightness against corpuscular bodies (viable bacteria) has been successfully investigated under static and dynamic conditions. the aim of this study was to investigate the tightness against endotoxins of two implant systems (astratech and ankylos) with conical internal connections under static conditions. the inner parts of eight implants of each ... | 2010 | 19629543 |
| rapid, sensitive, and specific detection of the o4 group of salmonella enterica by loop-mediated isothermal amplification. | the present study developed a loop-mediated isothermal amplification (lamp) assay that amplifies the fragments of o4 salmonella enterica-specific gene rfbj and evaluates the potential use in detection of salmonella enterica serovar typhimurium (st). the detection limit of the lamp assay was 10(3) cfu/ml, which was lower than that of the pcr assay with the same target gene (10(5) cfu/ml), confirmed by electrophoresis. the increased turbidity of the final products of lamp was also observed with mo ... | 2009 | 19630227 |
| salmonella enterica serovar typhi in japan, 2001-2006: emergence of high-level fluoroquinolone-resistant strains. | the phage types and antimicrobial susceptibilities of 226 isolates of salmonella enterica serovar typhi from imported cases in japan between 2001 and 2006 were investigated. most (93.8%) had travelled to asian countries, particularly south east asia. twenty-one phage types were identified with e1 (30.5%), uvs (15.9%) and b1 (9.3%) being the most common. the frequency of multidrug-resistant strains reached 37.0% in 2006 with phage types e1 and e9 predominating. almost half (48.2%) of the isolates ... | 2010 | 19631017 |
| emergence of fluoroquinolone-resistant strains of salmonella enterica in surabaya, indonesia. | typhoid fever remains a major health problem in developing countries. fluoroquinolones such as ciprofloxacin emerged as the 1st-choice treatment of enteric fever, including typhoid, in the 1990s. recently, salmonella typhi strains with resistance to ciprofloxacin have been increasingly reported in several countries, although the fluoroquinolone-resistant clinical strain has not been reported in indonesia. in the present study, we examined the drug susceptibility and the presence of gyra mutation ... | 2009 | 19631095 |
| rapid identification of salmonella enterica subsp. arizonae and s. enterica subsp. diarizonae by real-time polymerase chain reaction. | reptiles are popular as pets, leading to an increased risk of human infections due to uncommon salmonella strains including the arizona group (subspecies arizonae and diarizonae). we present a real-time arizona-specific polymerase chain reaction demonstrating 100% specificity and 99.6% sensitivity, offering savings in time and labor over traditional identification methods. | 2009 | 19631101 |
| innate immune gene expression differentiates the early avian intestinal response between salmonella and campylobacter. | salmonella enterica serovar typhimurium and campylobacter jejuni are major human pathogens, yet colonise chickens without causing pathology. the aim of this study was to compare intestinal innate immune responses to both bacterial species, in a 4-week-old broiler chicken model. challenged and control birds were sacrificed and tissue samples taken for histopathology and rna extraction. no significant clinical or pathological changes were observed in response to infection with either bacterial spe ... | 2009 | 19632728 |
| distribution of sulfonamide resistance genes in escherichia coli and salmonella isolates from swine and chickens at abattoirs in ontario and québec, canada. | sulfonamide-resistant escherichia coli and salmonella isolates from pigs and chickens in ontario and québec were screened for sul1, sul2, and sul3 by pcr. each sul gene was distributed differently across populations, with a significant difference between distribution in commensal e. coli and salmonella isolates and sul3 restricted mainly to porcine e. coli isolates. | 2009 | 19633109 |
| [a new method of designing genechip probe for frontier port detection of pathogenic microorganism]. | in the process of designing genechip probe for detecting pathogenic microorganism, the selection of single nucleotide polymorphism (snp) combination is of great importance. at present, there is no automatic design method. this work is hard and the result is not always well. a new approach for selecting snp combination is presented in this paper. genetic algorithm is used to search optimal solution on the basis of classification ability of snp combination, which is evaluated by the rough set theo ... | 2009 | 19634689 |
| nmr assignment of reduced form of copper, zinc superoxide dismutase from salmonella enterica. | almost complete assignment (97%) of nmr resonances was obtained for the reduced, cu(i), form of prokaryotic cuznsod from salmonella enterica. 13c direct detection was used to complement the standard bouquet of 1h detected triple resonance experiments and contributed to the identification of proline backbone resonances and to side chains assignments of asx, glx and aromatic rings. this is the only complete assignment available for monomer sod from prokaryotic organisms. | 2007 | 19636828 |
| development and application of an electrochemical plate coupled with immunomagnetic beads (elime) array for salmonella enterica detection in meat samples. | salmonella is one of the main organisms causing outbreaks of foodborne illness, and meat is one of the major vehicles of salmonellosis throughout the world. a novel analytical immunosensor array, based on a 96-well electrochemical plate coupled with immunomagnetic beads (elime array), is proposed for the detection of salmonella in meat samples. after an optimization study, using salmonella enterica serotype enteritidis as reference antigen, the ability of the method to interact with a large numb ... | 2009 | 19639993 |
| survival and growth of salmonella and vibrio in som-fak, a thai low-salt garlic containing fermented fish product. | fermentation of raw fish is a common process in asia for improvement of shelf life and safety, however, little is known about the survival of pathogenic bacteria in these products. raw fish may be contaminated with salmonella and vibrio species. the purpose of this study was to determine survival and potential growth of salmonella enterica serovar weltevreden, s. enterica serovar enteritidis, vibrio cholerae and v. parahaemolyticus as influenced by the preservation parameters (sodium chloride, g ... | 2009 | 19640599 |
| detection of enterobacter sakazakii and other pathogens associated with infant formula powder by use of a dna microarray. | pathogen detection is critical to the process of generating and testing powdered infant formula (pif). an obstacle associated with pif microbial surveillance is that most current procedures are time-consuming and labor-intensive. we have developed a rapid, dna microarray-based detection technique to identify 10 different pathogenic bacteria associated with pif contamination based on the 16s-23s rrna gene internal transcribed spacer (its) sequences and wzy (o antigen polymerase) gene. using this ... | 2009 | 19641057 |
| gamma radiation used as hygienization technique for foods does not induce viable but non-culturable state (vbnc) in salmonella enterica subsp. enterica serovar typhimurium. | gamma radiation has been widely used for hygienization of food products. whether gamma radiation stress induces vbnc state in salmonella is of great concern. therefore, the study was carried out to determine whether gamma radiation exposure induces vbnc state in salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium). the parameters tested were culturability on agar medium, transcriptional activity by rt-pcr, cytoplasmic membrane integrity, and direct viable count using live/dea ... | 2009 | 19641961 |
| salmonella enterica subclinical infection: bacteriological, serological, pulsed-field gel electrophoresis, and antimicrobial resistance profiles--longitudinal study in a three-site farrow-to-finish farm. | the aim of this surveillance was to study both salmonella spp. shedding patterns and the time course of serological response in farrow-to-finish reared pigs from a subclinically infected farm. antimicrobial resistance profile, molecular subtyping, and the relationship among the isolates were determined by pulsed-field gel electrophoresis (pfge). a farrow-to-finish farm of 6000 sows, with a history of salmonella typhimurium septicemia, was selected. a longitudinal bacteriological and serological ... | 2009 | 19642916 |
| transcriptional profiling avian beta-defensins in chicken oviduct epithelial cells before and after infection with salmonella enterica serovar enteritidis. | salmonella enterica serovar enteritidis (se) colonizes the ovary and oviduct of chickens without causing overt clinical signs which can lead to se-contamination of the content and membrane of shell-eggs as well as hatchery eggs. the organism utilizes the salmonella pathogenicity island-2 encoded type iii secretion system (t3ss-2) to promote persistence in the oviduct of laying hens. in this study, reverse transcriptase-polymerase chain reaction (rt-pcr) was carried out to determine the expressio ... | 2009 | 19642979 |
| internalization of salmonella enterica in leaves is induced by light and involves chemotaxis and penetration through open stomata. | outbreaks of salmonellosis related to consumption of fresh produce have raised interest in salmonella-plant interactions leading to plant colonization. incubation of gfp-tagged salmonella enterica with iceberg lettuce leaves in the light resulted in aggregation of bacteria near open stomata and invasion into the inner leaf tissue. in contrast, incubation in the dark resulted in a scattered attachment pattern and very poor stomatal internalization. forcing stomatal opening in the dark by fusicocc ... | 2009 | 19648358 |
| antimicrobial resistance-conferring plasmids with similarity to virulence plasmids from avian pathogenic escherichia coli strains in salmonella enterica serovar kentucky isolates from poultry. | salmonella enterica, a leading cause of food-borne gastroenteritis worldwide, may be found in any raw food of animal, vegetable, or fruit origin. salmonella serovars differ in distribution, virulence, and host specificity. salmonella enterica serovar kentucky, though often found in the food supply, is less commonly isolated from ill humans. the multidrug-resistant isolate s. kentucky cvm29188, isolated from a chicken breast sample in 2003, contains three plasmids (146,811 bp, 101,461 bp, and 46, ... | 2009 | 19648374 |
| comprehensive identification of salmonella enterica serovar typhimurium genes required for infection of balb/c mice. | genes required for infection of mice by salmonella typhimurium can be identified by the interrogation of random transposon mutant libraries for mutants that cannot survive in vivo. inactivation of such genes produces attenuated s. typhimurium strains that have potential for use as live attenuated vaccines. a quantitative screen, transposon mediated differential hybridisation (tmdh), has been developed that identifies those members of a large library of transposon mutants that are attenuated. tmd ... | 2009 | 19649318 |
| purification, crystallization and preliminary x-ray analysis of flit, a bacterial flagellar substrate-specific export chaperone. | the assembly process of the bacterial flagellum is coupled to flagellar gene expression. flit acts not only as a flagellar type iii substrate-specific export chaperone for the filament-capping protein flid but also as a negative regulator that suppresses flagellar gene expression through its specific interaction with the master regulator flhd(4)c(2) complex. in this study, flit of salmonella enterica serovar typhimurium was expressed, purified and crystallized. crystals of semet flit were obtain ... | 2009 | 19652350 |
| salmonella enterica serotypes and antibiotic susceptibility in new zealand, 2002-2007. | we analysed the serotypes and antibiotic susceptibility of 1560 human and 1505 non-human salmonella isolated in new zealand (nz) between 2002 and 2007. the most common serotypes in humans were salmonella enterica serovar typhimurium, s. enteritidis, s. brandenburg and s. infantis. over the 6-year period human cases due to s. agona and s. enteritidis increased and cases due to s. typhimurium decreased. the most common serotypes from non-human sources were s. typhimurium, s. brandenberg, s. hindma ... | 2010 | 19653924 |
| cell yields and fermentation responses of a salmonella typhimurium poultry isolate at different dilution rates in an anaerobic steady state continuous culture. | the objectives of these studies were to determine cell yield and fermentation responses of a salmonella enterica serovar typhimurium poultry isolate using various dilution rates in steady state continuous culture incubations. s. enterica typhimurium cells were propagated in continuous cultures with a total volume of 0.50 l of luria bertani medium containing 0.1% glucose. dilution rates from 0.0125 to 1.44/h were used. cell protein concentration generally increased linearly with increased dilutio ... | 2009 | 19655268 |
| molecular typing of escherichia coli strains isolated from food in casablanca (morocco). | serotyping of o- and h- antigens is regarded as the gold standard in classification of e. coli for taxonomic and epidemiological purposes similar to the kaufmann-white scheme for salmonella enterica. molecular methods to replace or to support the serotyping were recently applied. using the molecular polymorphism of the somatic (o- antigen) gene rfb cluster and flagella (h- antigen) gene flic, 74 e. coli strains carrying the virulent genes isolated from food were characterised by polymerase chain ... | 2009 | 19656466 |
| protection of salmonella by ampicillin-resistant escherichia coli in the presence of otherwise lethal drug concentrations. | microbial systems have become the preferred testing grounds for experimental work on the evolution of traits that benefit other group members. this work, based on conceptual and theoretical models of frequency-dependent selection within populations, has proven fruitful in terms of understanding the dynamics of group beneficial or 'public goods' traits within species. here, we expand the scope of microbial work on the evolution of group-beneficial traits to the case of multi-species communities, ... | 2009 | 19656787 |
| global transcriptional response of salmonella enterica serovar typhi to anti-z66 antiserum. | recent studies have shown that flagella may modulate physiological processes by sensing environmental changes in temperature and moisture. when the z66(+) strain of salmonella enterica serovar typhi (s. typhi) was exposed to an antiserum against the z66 flagellar antigen, the fljba operon was deleted from a linear plasmid, leading to the unidirectional flagellar phase variation from fljb to flic. we hypothesized that flagella may serve as a sensor that responded to the antiserum by altering gene ... | 2009 | 19659726 |
| outbreak of salmonella enterica serotype muenster infections associated with goat's cheese, france, march 2008. | salmonella enterica serotype muenster (hereafter referred to as s. muenster) is rare in france and in europe. in france, a nationwide outbreak of gastrointestinal illness due to s. muenster occurred during march and april 2008. twenty-five laboratory-confirmed cases of s. muenster were documented by telephone using a trawling questionnaire. four patients were admitted to hospital and no death was recorded. among the 21 interviewed cases, 16 reported consumption of goat's cheese in the days prior ... | 2009 | 19660242 |
| macrophages influence salmonella host-specificity in vivo. | the generalist salmonella enterica serovar typhimurium causes disease in many animal species, but the closely related host-specific serovar typhi only causes disease in humans. typhi and typhimurium share major virulence loci; hence it is not known exactly why typhi does not cause disease in mice. we tested the hypothesis that macrophages contribute to salmonella host-specificity in mice. no significant difference in survival of the two serovars was observed in vitro in mouse macrophage cell lin ... | 2009 | 19660538 |
| [activity of ertapenem and ceftriaxone in the eradication of salmonella in a model of experimental peritonitis in mice]. | since the beginning of the 1990s, the prevalence of isolates of non-typhoidal salmonella species resistant to antimicrobial agents, including those commonly used for the treatment of salmonella infections such as fluoroquinolones, trimethoprim-sulfamethoxazole or beta-lactams, has increased substantially. infections caused by multidrug-resistant strains of non-typhoidal salmonella are now frequently encountered. in order to consider an appropriate role of ertapenem in salmonella-due intraabdomin ... | 2009 | 19662546 |
| characterization of lactobacillus spp. isolated from the feces of breast-feeding piglets. | lactobacillus spp., referred to as ij-1 and ij-2, were isolated from the feces of breast-feeding piglets and analyzed for probiotic properties. according to the analyses of 16s rdna sequence, lactobacillus sp. ij-1 showed greater than 99% homology with lactobacillus reuteri dsm 20016(t), and lactobacillus sp. ij-2 had greater than 99% homology with the l. gasseri atcc 33323(t) and l. johnsonii atcc 33200(t). the ph changes in the culture media of lactobacillus sp. ij-1 and lactobacillus sp. ij-2 ... | 2009 | 19664551 |
| autonomous and flik-dependent length control of the flagellar rod in salmonella enterica. | salmonella flgg point mutations produce filamentous rod structures whose lengths are determined by flik. flik length variants produce rods with lengths proportional to the corresponding flik molecular size, suggesting that flik controls the length of not only the hook but also the rod by the same molecular mechanism. | 2009 | 19666714 |
| quantification of horizontal transmission of salmonella enterica serovar enteritidis bacteria in pair-housed groups of laying hens. | an important source of human salmonellosis is the consumption of table eggs contaminated with salmonella enterica serovar enteritidis. optimization of the various surveillance programs currently implemented to reduce human exposure requires knowledge of the dynamics of s. enteritidis infection within flocks. the aim of this study was to provide parameter estimates for a transmission model of s. enteritidis in laying-type chicken flocks. an experiment was carried out with 60 pairs of laying hens. ... | 2009 | 19666725 |
| autophagy genes protect against salmonella typhimurium infection and mediate insulin signaling-regulated pathogen resistance. | a conserved insulin-like pathway modulates both aging and pathogen resistance in caenorhabditis elegans. however, the specific innate effector functions that mediate this pathogen resistance are largely unknown. autophagy, a lysosomal degradation pathway, plays a role in controlling intracellular bacterial pathogen infections in cultured cells, but less is known about its role at the organismal level. we examined the effects of autophagy gene inactivation on salmonella enterica serovar typhimuri ... | 2009 | 19667176 |
| silica-antibiotic hybrid nanoparticles for targeting intracellular pathogens. | we investigated the capability of biodegradable silica xerogel as a novel carrier of antibiotic and the efficacy of treatment compared to that with the same dose of free drug against murine salmonellosis. the drug molecules (31%) entrapped in the sol-gel matrix remained in biologically active form, and the bactericidal effect was retained upon drug release. the in vitro drug release profiles of the gentamicin from the xerogel and that from the xerogel-polyethylene glycol (peg) were distinctly di ... | 2009 | 19667284 |
| polysaccharides derived from yamoa (funtumia elastica) prime gammadelta t cells in vitro and enhance innate immune responses in vivo. | yamoa (ground bark of funtumia elastica tree) is marketed and sold as a dietary supplement with anecdotal therapeutic effects in the treatment of asthma and hay fever. we determined that yamoa and yamoa-derived polysaccharides affected innate immunity, in part, by priming gammadelta t cells. gene expression patterns in purified bovine gammadelta t cells and monocytes induced by yamoa were similar to those induced by ultrapure lipopolysaccharide (ulps). in the presence of accessory cells, yamoa h ... | 2009 | 19671448 |
| an improved method to knock out the asd gene of salmonella enterica serovar pullorum. | an asd-deleted (deltaasd) mutant of salmonella enterica serovar pullorum (sp) was constructed using an improved method of gene knockout by combining the pi-suicide plasmid system with the red disruption system. the asd gene was efficiently knocked out by the recombinant suicide vector, which replaced the asd gene with the cmr gene. based on the balanced lethal host-vector system, the phenotype of the deltaasd mutant was further defined. the improved method was simpler and more effective than pre ... | 2009 | 19672470 |
| use of stabilized luciferase-expressing plasmids to examine in vivo-induced promoters in the vibrio cholerae vaccine strain cvd 103-hgr. | live, attenuated vibrio cholerae vaccines can induce potent immune responses after only a single oral dose. the strategy of harnessing these strains to present antigens from heterologous pathogens to the mucosal immune system shows great promise. to fully realize this possibility, v. cholerae strains must be created that stably express antigens in vivo in sufficient quantity to generate an immune response. in vivo-induced promoters have been shown to increase the stability and immunogenicity of ... | 2009 | 19678844 |
| escherichia coli unsaturated fatty acid synthesis: complex transcription of the faba gene and in vivo identification of the essential reaction catalyzed by fabb. | although the unsaturated fatty acid (ufa) synthetic pathway of escherichia coli is the prototype of such pathways, several unresolved issues have accumulated over the years. the key players are the faba and fabb genes. earlier studies of faba transcription showed that the gene was transcribed from two promoters, with one being positively regulated by the fadr protein. the other weaker promoter (which could not be mapped with the technology then available) was considered constitutive because its ... | 2009 | 19679654 |
| antibody selection for immobilizing living bacteria. | we report a comparative study of the efficacy of immobilizing living bacteria by means of seven antibodies against bacterial surface antigens associated with salmonella enterica serovar typhimurium. the targeted bacterial antigens were cfa/i fimbriae, flagella, lipopolysaccharides (lps), and capsular f1 antigen. the best immobilization of s. typhimurium was achieved with the antibody against cfa/i fimbriae. the immobilization of bacteria using antiflagellin showed significant enhancement if the ... | 2009 | 19681578 |
| genomic diversity of citrate fermentation in klebsiella pneumoniae. | it has long been recognized that klebsiella pneumoniae can grow anaerobically on citrate. genes responsible for citrate fermentation of k. pneumoniae were known to be located in a 13-kb gene cluster on the chromosome. by whole genome comparison of the available k. pneumoniae sequences (mgh 78578, 342, and ntuh-k2044), however, we discovered that the fermentation gene cluster was present in mgh 78578 and 342, but absent in ntuh-k2044. in the present study, the previously unknown genome diversity ... | 2009 | 19682387 |
| role of a conserved prolyl residue (pro173) of mota in the mechanochemical reaction cycle of the proton-driven flagellar motor of salmonella. | the mota/b complex acts as the stator of the proton-driven bacterial flagellar motor. proton translocation through the stator complex is efficiently coupled with torque generation by the stator-rotor interactions. in salmonella enterica serovar typhimurium, the highly conserved pro173 residue of mota is close to the absolutely conserved asp33 residue of motb, which is believed to be a proton-binding site. pro173 is postulated to be involved in coupling proton influx to torque generation. however ... | 2009 | 19683537 |
| the s. typhimurium effector sope induces caspase-1 activation in stromal cells to initiate gut inflammation. | in the healthy intestinal mucosa, homeostasis between the immune system and commensal microflora prevents detrimental inflammatory responses. infection with acute enteropathogens such as salmonella enterica serovar typhimurium disturbs this homeostasis and triggers inflammation, but the underlying mechanisms are poorly understood. we found that bacterial delivery or ectopic expression of the s. typhimurium type iii effector protein sope, a known activator of host cellular rho gtpases, led to pro ... | 2009 | 19683679 |
| prognostic factors and clinical features of non-typhoid salmonella bacteremia in adults. | infections caused by non-typhoid salmonella (nts) have increased, and the increasing incidence of multidrug-resistant nts bacteremia in adult patients has also been noted. this study aimed to investigate the clinical and microbiological characteristics of nts bacteremia. | 2009 | 19686996 |
| molecular insights into farm animal and zoonotic salmonella infections. | salmonella enterica is a facultative intracellular pathogen of worldwide importance. infections may present in a variety of ways, from asymptomatic colonization to inflammatory diarrhoea or typhoid fever depending on serovar- and host-specific factors. human diarrhoeal infections are frequently acquired via the food chain and farm environment by virtue of the ability of selected non-typhoidal serovars to colonize the intestines of food-producing animals and contaminate the avian reproductive tra ... | 2009 | 19687040 |
| pspa family fusion proteins delivered by attenuated salmonella enterica serovar typhimurium extend and enhance protection against streptococcus pneumoniae. | pneumococcal surface protein a (pspa) is highly immunogenic and can induce a protective immune response against pneumococcal infection. pspa is divided into two major families based on serological variability: family 1 and family 2. to provide broad protection, pspa proteins from pneumococcal strains rx1 (family 1) and ef5668 (family 2) were combined to form two pspa fusion proteins, pspa/rx1-ef5668 and pspa/ef5668-rx1. each protein was fused to a type ii secretion signal and delivered by a reco ... | 2009 | 19687204 |
| msbb deletion confers acute sensitivity to co2 in salmonella enterica serovar typhimurium that can be suppressed by a loss-of-function mutation in zwf. | pathogens tolerate stress conditions that include low ph, oxidative stress, high salt and high temperature in order to survive inside and outside their hosts. lipopolysaccharide (lps), which forms the outer-leaflet of the outer membrane in gram-negative bacteria, acts as a permeability barrier. the lipid a moiety of lps anchors it to the outer membrane bilayer. the msbb enzyme myristoylates the lipid a precursor and loss of this enzyme, in salmonella, is correlated with reduced virulence and sev ... | 2009 | 19689794 |
| salmonella type iii secretion effector slrp is an e3 ubiquitin ligase for mammalian thioredoxin. | salmonella enterica encodes two virulence-related type iii secretion systems in salmonella pathogenicity islands 1 and 2, respectively. these systems mediate the translocation of protein effectors into the eukaryotic host cell, where they alter cell signaling and manipulate host cell functions. however, the precise role of most effectors remains unknown. using a genetic screen, we identified the small, reduction/oxidation-regulatory protein thioredoxin as a mammalian binding partner of the salmo ... | 2009 | 19690162 |
| cyclic di-gmp signalling controls virulence properties of salmonella enterica serovar typhimurium at the mucosal lining. | cyclic di-gmp (c-di-gmp), a novel secondary signalling molecule present in most bacteria, controls transition between motility and sessility. in salmonella enterica serovar typhimurium (s. typhimurium) high c-di-gmp concentrations favour the expression of a biofilm state through expression of the master regulator csgd. in this work, we investigate the effect of c-di-gmp signalling on virulence phenotypes of s. typhimurium. after saturation of the cell with c-di-gmp by overexpression of a di-guan ... | 2010 | 19691499 |
| selectively reduced intracellular proliferation of salmonella enterica serovar typhimurium within apcs limits antigen presentation and development of a rapid cd8 t cell response. | ag presentation to cd8(+) t cells commences immediately after infection, which facilitates their rapid expansion and control of pathogen. this paradigm is not followed during infection with virulent salmonella enterica serovar typhimurium (st), an intracellular bacterium that causes mortality in susceptible c57bl/6j mice within 7 days and a chronic infection in resistant mice (129 x 1svj). infection of mice with ova-expressing st results in the development of a cd8(+) t cell response that is det ... | 2009 | 19692639 |
| tumor invasion of salmonella enterica serovar typhimurium is accompanied by strong hemorrhage promoted by tnf-alpha. | several facultative anaerobic bacteria with potential therapeutic abilities are known to preferentially colonize solid tumors after systemic administration. how they efficiently find and invade the tumors is still unclear. however, this is an important issue to be clarified when bacteria should be tailored for application in cancer therapy. | 2009 | 19693266 |
| a comparative study of thermal and acid inactivation kinetics in fruit juices of salmonella enterica serovar typhimurium and salmonella enterica serovar senftenberg grown at acidic conditions. | acid and heat inactivation in orange and apple juices of salmonella enterica serovar typhimurium colección española de cultivos tipo (i.e., spanish type culture collection) 443 (cect 443) (salmonella typhimurium) and s. enterica serovar senftenberg cect 4384 (salmonella senftenberg) grown in buffered brain heart infusion (ph 7.0) and acidified brain heart infusion up to ph 4.5 with acetic, citric, lactic, and hydrochloric acids was evaluated. acid adaptation induced an adaptive response that inc ... | 2009 | 19694554 |
| salmonella enterica serotype typhimurium dt104 arta-dependent modification of pertussis toxin-sensitive g proteins in the presence of [32p]nad. | salmonella enterica serotype typhimurium (s. typhimurium) definitive phage type (dt) 104 has become a widespread cause of human and other animal infections worldwide. the severity of clinical illness in s. typhimurium dt104 outbreaks suggests that this strain possesses enhanced virulence. arta and artb - encoded by a prophage in s. typhimurium dt104 - are homologues of components of pertussis toxin (ptx), including its adp-ribosyltransferase subunit. here, we show that exposing dt104 to mitomyci ... | 2009 | 19696112 |
| development of an indirect elisa for the detection of serum igg antibodies against region iv of phase 1 flagellin of salmonella enterica serovar brandenburg in sheep. | region iv of phase 1 flagellin (flic) of salmonella enterica serovar brandenburg (s. brandenburg) was expressed in escherichia coli and purified by nickel chelate affinity chromatography. the purified recombinant protein was evaluated for its suitability as an antigen in an indirect elisa for the detection of antibodies in sheep sera. a cut-off value of 0.1 was calculated using 80 serum samples collected from sheep with no previous history of s. brandenburg. in the present study we show the resu ... | 2009 | 19696151 |
| plasmid-mediated 16s rrna methylases among extended-spectrum-beta-lactamase-producing salmonella enterica senftenberg isolates from algeria. | 2009 | 19700474 | |
| cloning of salmonella enterica serovar enteritidis fimbrial protein sefa as a surface protein in escherichia coli confers the ability to attach to eukaryotic cell lines. | the gene for the salmonella enterica serovar enteritidis fimbrial protein sefa was cloned into an escherichia coli surface expression vector and confirmed by western blot assay. e. coli clones expressing sefa attached to avian ovary granulosa cells and hep-2 cells, providing evidence for the involvement of sefa in the ability of salmonella to attach to eukaryotic cells. | 2009 | 19700549 |
| absence of functional hfe protects mice from invasive salmonella enterica serovar typhimurium infection via induction of lipocalin-2. | mutations of hfe are associated with hereditary hemochromatosis, but their influence on host susceptibility to infection is incompletely understood. we report that mice lacking one or both hfe alleles are protected from septicemia with salmonella typhimurium, displaying prolonged survival and improved control of bacterial replication. this increased resistance is paralleled by an enhanced production of the enterochelin-binding peptide lipocalin-2 (lcn2), which reduces the availability of iron fo ... | 2009 | 19700664 |
| introduction of new multidrug-resistant salmonella enterica strains into commercial dairy herds. | a longitudinal observational study of 59 dairy herds was conducted in washington state to estimate the rate of introduction of new multidrug-resistant (mdr) salmonella enterica strains onto commercial dairy herds. samples were collected on these herds over 7 visits separated by intervals of 2 to 4 mo over a period of 15 to 21 mo. samples were cultured for salmonella spp. and serogroup, serovar, and antimicrobial susceptibility patterns were identified for mdr salmonella isolates. fingerprinting ... | 2009 | 19700683 |
| survey of probiotic enterococci from traditional dairy products and their impact upon the infant intestinal microbiota. | 2009 | 19700927 | |
| quinolone-resistant salmonella enterica serovar typhi presenting as acute fulminant hepatitis. | 2009 | 19702041 | |
| the tvia auxiliary protein renders the salmonella enterica serotype typhi rcsb regulon responsive to changes in osmolarity. | in response to osmolarity, salmonella enterica serotype typhi (s. typhi) regulates genes required for vi capsular antigen expression oppositely to those required for motility and invasion. previous studies suggest that osmoregulation of motility, invasion and capsule expression is mediated through the rcsc/rcsd/rcsb phosphorelay system. here we performed gene expression profiling and functional studies to determine the role of tvia, an auxiliary protein of the rcsb response regulator, in control ... | 2009 | 19703107 |
| rcs signalling-activated transcription of rcsa induces strong anti-sense transcription of upstream flipqr flagellar genes from a weak intergenic promoter: regulatory roles for the anti-sense transcript in virulence and motility. | in salmonella enterica, an activated rcs signalling system inhibits initiation of transcription of the flhd master operon. under these conditions, where motility is shut down, microarray experiments showed an increased rna signal for three flagellar genes -flipqr- located upstream of rcsa. we show here that it is the anti-sense (as) strand of these genes that is transcribed, originating at a weak promoter in the intergenic region between flir and rcsa. rcsa is an auxiliary regulator for the rcs ... | 2009 | 19703110 |
| national surveillance of salmonella enterica in food-producing animals in japan. | a total of 518 fecal samples collected from 183 apparently healthy cattle, 180 pigs and 155 broilers throughout japan in 1999 were examined to determine the prevalence and antimicrobial susceptibility of salmonella. the isolation rates were 36.1% in broilers, 2.8% in pigs and 0.5% in cattle. s. enterica infantis was the most frequent isolate, found in 22.6% of broiler fecal samples. higher resistance rates were observed against oxytetracycline (82.0%), dihydrostreptomycin (77.9%), kanamycin (41. ... | 2009 | 19703311 |
| involvement of spi-2-encoded spic in flagellum synthesis in salmonella enterica serovar typhimurium. | spic encoded within salmonella pathogenicity island 2 on the salmonella enterica serovar typhimurium chromosome is required for survival within macrophages and systemic infection in mice. additionally, spic contributes to salmonella-induced activation of the signal transduction pathways in macrophages by affecting the expression of flic, a component of flagella filaments. here, we show the contribution of spic in flagellum synthesis. | 2009 | 19706157 |
| an outer membrane protease of the omptin family prevents activation of the citrobacter rodentium phopq two-component system by antimicrobial peptides. | the phopq two-component system of the intracellular pathogen salmonella enterica senses and controls resistance to alpha-helical antimicrobial peptides (amps) by regulating covalent modifications of lipid a. a homologue of the phopq operon was found in the genome of the murine enteric extracellular pathogen, citrobacter rodentium. here we report that c. rodentium phopq was apparently unable to mediate activation of target genes in the presence of alpha-helical amps. however, these amps activated ... | 2009 | 19708916 |
| influence of mutation frequency on the persistence of salmonella enterica serotypes in natural waters. | abstract in aquatic environments, the survival of pathogenic microorganisms, such as salmonella spp., may represent a risk to public health. the mutation frequency was hypothesized to influence the persistence of salmonella serotypes in environmental waters. the mean mutation frequency to rifampin resistance of the 11 major serotypes of salmonella enterica isolated from the tech river (pyrénées-orientales, france) was determined. then, a comparison of the survival of three serotypes holding diff ... | 2002 | 19709246 |
| interaction between host cells and septicemic salmonella enterica serovar typhimurium isolates from pigs. | salmonella enterica serovar typhimurium is an important pathogen in swine and is also a frequently reported zoonotic agent. the objective of this study was to characterize isolates of s. enterica serovar typhimurium associated with septicemia in swine and to compare them to isolates recovered from clinically healthy pigs. we were particularly interested in comparing the two groups of isolates for their ability to adhere to and invade host cells, to be phagocytized and survive in monocyte cells, ... | 2009 | 19710281 |
| kinetics of the natural, humoral immune response to salmonella enterica serovar typhi in kathmandu, nepal. | typhoid fever is a major public health problem in developing countries, conservatively estimated to occur in 17 million cases and be responsible for 200,000 deaths annually. we investigated the acquisition of natural immunity to salmonella enterica serovar typhi in a region where typhoid is endemic by testing sera from an age-stratified sample of 210 healthy participants in kathmandu, nepal, for bactericidal activity toward s. typhi and for anti-vi capsular polysaccharide antibodies. bactericida ... | 2009 | 19710294 |
| effect of mixed antimicrobial agents and flavors in active packaging films. | active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ... | 2009 | 19711918 |
| previously uncharacterized salmonella enterica genes required for swarming play a role in seedling colonization. | incidences of bacterial foodborne illness caused by ingestion of fresh produce are rising. instead of this being due to incidental contamination, the animal pathogen salmonella enterica utilizes specific molecular mechanisms to attach to and colonize plants. this work characterizes two s. enterica genes of unknown function: a putative periplasmic protein, stm0278, and a putative protein with a hydrolase in the c-terminus, stm0650. stm0278 and stm0650 are important for seedling colonization but a ... | 2009 | 19713240 |
| an enteric pathogen salmonella enterica serovar typhimurium suppresses tumor growth by downregulating cd44high and cd4t regulatory (treg) cell expression in mice: the critical role of lipopolysaccharide and braun lipoprotein in modulating tumor growth. | an antitumor activity associated with several bacterial pathogens, including salmonella enterica serovar typhimurium, has been reported; however, the underlying immunological mechanism(s) that lead to an antitumor effect are currently unclear. furthermore, such pathogens cannot be used to suppress tumor growth because of their potential for causing sepsis. recently, we reported the characterization of s. typhimurium isogenic mutants from which braun lipoprotein genes (lppa and b) and the multico ... | 2010 | 19713997 |
| transepithelial activation of human leukocytes by probiotics and commensal bacteria: role of enterobacteriaceae-type endotoxin. | the goal of the current study was to clarify whether commercially available probiotics induce greater trans-epithelial activation of human leukocytes than do commensal, food-derived and pathogenic bacteria and to identify the compounds responsible for this activation. eleven different bacterial strains, and some of their pathogen-associated molecular patterns, were incubated apically on a confluent layer of intestinal epithelial cells (caco-2), which were basolaterally co-cultured with human mon ... | 2009 | 19714861 |
| real-time reverse-transcriptase polymerase chain reaction for the rapid detection of salmonella using inva primers. | recent outbreaks of salmonella linked to fresh produce emphasize the need for rapid detection methods to help control the spread of disease. reverse-transcriptase polymerase chain reaction (rt-pcr) can detect the presence of mrna (shorter half-life than dna) with greater potential for detecting viable pathogens. the chromosomally located inva gene required for host invasion by salmonella is widely used for detection of this pathogen by pcr. detection of salmonella was undertaken by real-time rt- ... | 2009 | 19715427 |
| acute acalculous cholecystitis complicating typhoid fever in an adult patient: a case report and review of the literature. | a case of typhoidal acalculous cholecystitis is described in a 31-year-old indian man, who was admitted with 4-day fever, abdominal pain, diarrhea and vomiting. on examination, he looked ill, but was conscious and febrile with icteric sclera. the right upper quadrant of the abdomen was tender. investigations showed high liver enzymes with high total bilirubin. abdominal ultrasound findings were consistent with the diagnosis of acalculous cholecystitis and salmonella enterica serovar typhi was is ... | 2009 | 19717100 |
| crystal structure of the salmonella enterica serovar typhimurium virulence factor srfj, a glycoside hydrolase family enzyme. | to cause infection, salmonella enterica serovar typhimurium uses type iii secretion systems, which are encoded on two salmonella pathogenicity islands, spi-1 and spi-2, the latter of which is thought to play a crucial role in bacterial proliferation in salmonella-containing vacuoles (scvs) after invading cells. s. typhimurium srfj, located outside spi-2, is also known to be involved in salmonella pathogenicity and has high amino acid sequence homology with human lysosomal glucosylceramidase (glc ... | 2009 | 19717598 |
| dam methylation controls o-antigen chain length in salmonella enterica serovar enteritidis by regulating the expression of wzz protein. | we reported previously that a salmonella enterica serovar enteritidis dam mutant expressing a truncated dam protein does not agglutinate in the presence of specific antibodies against o9 polysaccharide. here we investigate the participation of dam in lipopolysaccharide (lps) synthesis in salmonella. the lps o-antigen profiles of a dam null mutant (sedeltadam) and the salmonella serovar enteritidis parental strain were examined by using electrophoresis and silver staining. compared to the parenta ... | 2009 | 19717610 |
| inactivation of salmonella enterica serovar enteritidis on shell eggs by pulsed light technology. | this is a study on the efficacy of pulsed light (pl) technology for the inactivation of salmonella enterica serovar enteritidis on shell eggs. in preliminary studies on noble agar, a pl treatment of 0.7 j/cm(2) gave an inactivation of 6.7 log cfu/cm(2). photoreactivation of salmonella (0.5-0.7 log cfu/cm(2)) was observed. different results were obtained in eggs according to the state of the cuticle. when unwashed eggs were pulsed, 24 to 80% of the samples showed the maximum decontamination (3.6 ... | 2009 | 19720417 |
| salmonella enterica serovar choleraesuis derivatives harbouring deletions in rpos and phop regulatory genes as vehicles for dna vaccines. | we investigated the use of two previously described attenuated strains of salmonella enterica subspecies enterica serovar choleraesuis (s. choleraesuis), deltaphop and deltarpos, compared with the commercial attenuated sc-54 strain, as bactofection vehicles, to deliver an epitope model (3xflag) to the intestinal immune system. the gene encoding the epitope 3xflag was subcloned into the pcmvbetam2a mammalian expression vector (creating pcmv3xflagm2a) and introduced into s. choleraesuis strains. t ... | 2010 | 19720478 |
| bacterial nitric oxide detoxification prevents host cell s-nitrosothiol formation: a novel mechanism of bacterial pathogenesis. | s-nitrosylation is an important mediator of multiple nitric oxide-dependent biological processes, including eukaryotic cellular events such as macrophage apoptosis and proinflammatory signaling. many pathogenic bacteria possess no detoxification mechanisms, such as the nitric oxide reductase (norb) of neisseria meningitidis and the flavohemoglobins (hmp) of salmonella enterica and escherichia coli, which serve to protect the microorganism from nitrosative stress within the intracellular environm ... | 2010 | 19720623 |
| salmonella enterica serovar typhimurium exploits toll-like receptor signaling during the host-pathogen interaction. | salmonella survives and replicates in host cells by using a type iii secretion system to evade host immune defenses. the innate immune system plays an important role as a first line of defense against pathogens and is mediated in part by toll-like receptors (tlrs); however, the infection dynamics of salmonella enterica serovar typhimurium within macrophages stimulated with tlr ligands is poorly understood. we studied the infection dynamics of salmonella in murine macrophages previously exposed t ... | 2009 | 19720755 |
| perceptions, practices, and consequences associated with foodborne pathogens and the feeding of raw meat to dogs. | this study explored the impact of feeding raw meat to dogs on the fecal prevalence of several enteric bacterial zoonotic pathogens. campylobacter jejuni was isolated from 1/42 (2.6%) raw meat-fed dogs. salmonella enterica was isolated from 2/40 (5%) of the raw meat feeds, 6/42 (14%) raw meat-fed dog feces, none of the dogs that did not receive raw meat (p = 0.001), 4/38 (10.5%) of the vacuum cleaner waste samples from households where raw meat was fed, and 2/44 (4.5%) of vacuum cleaner waste sam ... | 2009 | 19721784 |
| validation of intervention strategies to control escherichia coli o157:h7 and salmonella typhimurium dt 104 in mechanically tenderized and brine-enhanced beef. | after three different outbreaks were linked to the consumption of nonintact meat products contaminated with escherichia coli o157:h7, the u.s. department of agriculture, food safety and inspection service published notice requiring establishments producing mechanically tenderized and moisture-enhanced beef products to reassess their respective hazard analysis and critical control point system, due to potential risk to the consumers. the purpose of this study was to determine the effectiveness of ... | 2009 | 19722392 |
| sporadic salmonella enterica serotype javiana infections in georgia and tennessee: a hypothesis-generating study. | from 1996 to 2004, the incidence of salmonella javiana infections increased in foodnet, the u.s. national active foodborne disease surveillance programme. contact with amphibians and consumption of tomatoes have been associated with outbreaks of s. javiana infection. to generate and test hypotheses about risk factors associated with sporadic s. javiana infections, we interviewed patients with laboratory-confirmed s. javiana infection identified in georgia and tennessee during august-october 2004 ... | 2010 | 19723360 |
| comparison of the entericbio multiplex pcr system with routine culture for detection of bacterial enteric pathogens. | the entericbio system uses a multiplex pcr assay for the simultaneous detection of campylobacter spp., salmonella enterica, shigella spp., and escherichia coli o157 from feces. it combines overnight broth enrichment with pcr amplification and detection by hybridization. an evaluation of this system was conducted by comparing the results obtained with the system with those obtained by routine culture, supplemented with alternative pcr detection methods. in a study of 773 samples, routine culture ... | 2009 | 19726596 |
| use of toll-like receptor assays to detect and identify microbial contaminants in biological products. | toll-like receptor (tlr)-expressing cells, for the first time, detected and identified a microbial contaminant in a product made in escherichia coli using an old manufacturing process. it was suspected of having a microbial contaminant(s) because, although it tested negative by standard pyrogen assays, it was associated with adverse events in early clinical trials. the assay readout is the induction of nf-kappab and/or cytokines in response to tlr activation. four coded samples, labeled a to d, ... | 2009 | 19726599 |
| regulation of sulfur assimilation pathways in salmonella enterica serovar typhi upon up-shift high osmotic treatment: the role of uhpa revealed through transcriptome profiling. | salmonella species were the most deeply and extensively studied prokaryotes, which were used as useful prokaryotic models for the genetic analysis. salmonella enterica serovar typhi (s. typhi) is the etiological agent of typhoid fever, a major health problem in developing countries. uhpb/uhpa couple is a member of two-component regulatory system. it is considered that uhpb/uhpa controls the expression of the transport protein uhpt, which enables the cell to acquire phosphorylated sugars from its ... | 2009 | 19727945 |
| motility revertants of opggh mutants of salmonella enterica serovar typhimurium remain defective in mice virulence. | we recently demonstrated that osmoregulated periplasmic glucans (opgs) of salmonella enterica serovar typhimurium are required for optimal mouse virulence. however, lack of opgs also generated pleiotropic phenotypes such as reduced motility and slower growth rate under hypoosmotic growth conditions. whether the observed suboptimal virulence of opg mutants was due to reduced motility was investigated by isolating fully motile revertants of opggh mutants. motility revertants remained defective in ... | 2009 | 19727946 |
| effect of age on susceptibility to salmonella typhimurium infection in c57bl/6 mice. | ageing is associated with a decline in immune function, which predisposes the elderly to a higher incidence of infections. information on the mechanism of the age-related increase in susceptibility to salmonella enterica serovar typhimurium (s. typhimurium) is limited. in particular, little is known regarding the involvement of the immune response in this age-related change. we employed streptomycin (sm)-pretreated c57bl/6 mice to develop a mouse model that would demonstrate age-related differen ... | 2009 | 19729455 |
| o-antigen delays lipopolysaccharide recognition and impairs antibacterial host defense in murine intestinal epithelial cells. | although toll-like receptor (tlr) 4 signals from the cell surface of myeloid cells, it is restricted to an intracellular compartment and requires ligand internalization in intestinal epithelial cells (iecs). yet, the functional consequence of cell-type specific receptor localization and uptake-dependent lipopolysaccharide (lps) recognition is unknown. here, we demonstrate a strikingly delayed activation of iecs but not macrophages by wildtype salmonella enterica subsp. enterica sv. (s.) typhimur ... | 2009 | 19730692 |
| flow cytometric characterization of peyer's patch and cecal tonsil t lymphocytes in laying hens following challenge with salmonella enterica serovar enteritidis. | two trials were conducted to determine t cell changes in peyer's patches (pp) and cecal tonsils (ct) of specific-pathogen-free single-comb white leghorn hens challenged with salmonella enterica serovar enteritidis (se). each week, crop lavage samples were obtained from 4 or 3 hens in trials 1 and 2, respectively. these birds were then sacrificed and their intestinal tracts excised. the crop sample and contents of one cecum from each hen were cultured for the presence of se. cells were purified f ... | 2010 | 19732961 |
| intracellular concentrations of enrofloxacin in quinolone-resistant salmonella enterica subspecies enterica serovar choleraesuis. | the emergence of fluoroquinolone-resistant strains of salmonella enterica subspecies enterica serovar choleraesuis is an important concern in several countries, including japan. we examined the intracellular concentration of enrofloxacin in s. choleraesuis to determine the existence of a relationship with the emergence of quinolone resistance. the intracellular concentration of enrofloxacin was significantly lower in nalidixic acid-resistant isolates compared with nalidixic acid-susceptible isol ... | 2009 | 19733466 |
| genotypic characterization of antibiotic-resistant salmonella enteritidis isolates in dakar, senegal. | it is well established that salmonella enterica is a major cause of food-borne disease worldwide. in africa, according to the who global salm-surv country data bank from 2000 to 2002 salmonella enterica serovar enteritidis was the most common serotype involved in human salmonellosis. in dakar this serotype of salmonella has been reported as a frequent and an increasing cause of human infection. | 2007 | 19734606 |
| feeding the bt cationic peptides to chickens at hatch reduces cecal colonization by salmonella enterica serovar enteritidis and primes innate immune cell functional activity. | the bt/tamus 2032 (bt) cationic peptides are a group of related cationic peptides produced by a gram-positive soil bacterium, brevibacillus texasporus. cationic amphiphilic peptides produced by host cells have been found to stimulate or prime the innate immune responses in mammals, but little information is available on the effects of bacterial-produced peptides on host immunity. we have previously shown that bt, provided as a feed additive for 4 days after hatch, significantly induced protectio ... | 2010 | 19735207 |