Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| what sets bacillus anthracis apart from other bacillus species? | bacillus anthracis is the cause of anthrax, and two large plasmids are essential for toxicity: pxo1, which contains the toxin genes, and pxo2, which encodes a capsule. b. anthracis forms a highly monomorphic lineage within the b. cereus group, but strains of bacillus thuringiensis and b. cereus exist that are genetically closely related to the b. anthracis cluster. during the past five years b. cereus strains that contain the pxo1 virulence plasmid were discovered, and strains with both pxo1 and ... | 2009 | 19514852 |
| the catalytic property of 3-hydroxyisobutyrate dehydrogenase from bacillus cereus on 3-hydroxypropionate. | the mmsb gene product from bacillus cereus atcc14579 exhibits 3-hydroxypropionate dehydrogenase activity. it encodes the 32-kda enzyme protein composed of 292 amino acids. recombinant 3-hydroxyisobutyrate dehydrogenase (3-hibadh) was purified 100-fold from cell extract by ammonium sulfate fractionation and column chromatography. the enzyme catalyzed oxidation of 3-hydroxypropionate (3-hp) between ph 7.0 and 10.0 with optimal activity between 8.8 and 9.0. a km of 16.8 mm for 3-hp was calculated f ... | 2010 | 19517068 |
| inhibitory effect of commercial green tea and rosemary leaf powders on the growth of foodborne pathogens in laboratory media and oriental-style rice cakes. | the antimicrobial effects of green tea and rosemary added to foods as antagonists to foodborne pathogens were determined in laboratory media and oriental-style rice cakes. the growth of each pathogen (bacillus cereus, salmonella typhimurium, enterobacter sakazakii, escherichia coli o157:h7, staphylococcus aureus, and listeria monocytogenes) in tryptic soy broth or rice cake with or without addition of green tea or rosemary leaf powders before autoclaving or cooking, respectively, was investigate ... | 2009 | 19517743 |
| assessment of the microbiological safety of salad vegetables and sauces from kebab take-away restaurants in the united kingdom. | the purpose of this study was to establish the microbiological safety of salad vegetables and sauces served in kebab take-away restaurants. comparison with published microbiological guidelines revealed that 4.7% of 1213 salad vegetable samples were of unsatisfactory microbiological quality due to escherichia coli and/or staphylococcus aureus levels at > or =10(2) cfu g(-1). another 0.3% of salad samples were of unacceptable quality due to s. aureus at > or =10(4) cfu g(-1) (2 samples) or the pre ... | 2009 | 19527831 |
| bacillus cereus meningitis in a term neonate. | a case of bacillus cereus meningitis in a term neonate without identifiable risk factors or source of the organism is presented. prompt identification of the organism and treatment resulted in a favourable outcome. | 2009 | 19530006 |
| bacillus thuringiensis bel protein enhances the toxicity of cry1ac protein to helicoverpa armigera larvae by degrading insect intestinal mucin. | bacillus thuringiensis has been used as a bioinsecticide to control agricultural insects. bacillus cereus group genomes were found to have a bacillus enhancin-like (bel) gene, encoding a peptide with 20 to 30% identity to viral enhancin protein, which can enhance viral infection by degradation of the peritrophic matrix (pm) of the insect midgut. in this study, the bel gene was found to have an activity similar to that of the viral enhancin gene. a bel knockout mutant was constructed by using a p ... | 2009 | 19542344 |
| screening of some kenyan medicinal plants for antibacterial activity. | eleven medicinal plants used by traditional healers in machakos and kitui district were screened, namely: ajuga remota benth, aloe secundiflora engl, amaranthus hybridus l, cassia didymobotrya fes, croton macrostachyus del, entada leptostachya harms, erythrina abyssinica dc, harrisonia abyssinica oliv, schkuhria pinnata o. ktze, terminalia kilimandscharica engl and ziziphus abyssinica hochst for potential antibacterial activity against four medically important bacterial strains, namely: bacillus ... | 2010 | 19548257 |
| synthesis, characterization, antimicrobial activity and structure-activity relationships of new aryldisulfonamides. | a series of aromatic disulfonamide (1-8) derivatives and 4-methylbenzenesulfonyl hydrazide (9) were synthesized and characterized. all compounds were evaluated in vitro for their antimicrobial activity against staphylococcus aureus atcc 25953, bacillus cereus atcc 6633, bacillus magaterium rskk 5117, escherichia coli atcc 11230, salmonella enterititis atcc 13076 by microdilution and disc diffusion methods. antimicrobial activity of the aromatic disulfonamides decreased as the length of the carbo ... | 2009 | 19548771 |
| infections in patients with aplastic anemia. | infection is a major cause of death in patients with aplastic anemia (aa). there are differences between the immunocompromised state of a patient with aa and the patient who is neutropenic due to chemotherapy and this leads to a difference in the infections that they incur. prolonged neutropenia is one of the largest risk factors for the development of infections with the invasive mycoses and bacteria. recovery from neutropenia is directly related to survival, and supportive care plays a large r ... | 2009 | 19549579 |
| a dna microarray facilitates the diagnosis of bacillus anthracis in environmental samples. | in order to improve the diagnosis of bacillus anthracis in environmental samples, we established a dna microarray based on the arraytube technology of clondiag. | 2009 | 19552771 |
| comparison of multiplex pcr, enzyme immunoassay and cell culture methods for the detection of enterotoxinogenic bacillus cereus. | fast and reliable methods are needed for the detection of pathogenic bacillus cereus which should provide consistent results. therefore, we tested a panel of 176 strains, including b. cereus strains, b. cereus group strains and other bacillus spp. with polymerase chain reaction, immunoassays and cytotoxicity tests and assessed the consistency of the results. a screening multiplex pcr for the detection of hbl, nhe, ces and cytk1 as well as two multiplex pcrs for the differentiation of hbl genes ( ... | 2009 | 19555727 |
| phylogenetic analysis of bacillus p450 monooxygenases and evaluation of their activity towards steroids. | cytochrome p450 (p450) open reading frames (orfs) identified in genome sequences of bacillus species are potential resources for new oxidation biocatalysts. phylogenetic analysis of 29 bacillus p450 orfs revealed that the p450s consist of a limited number of p450 families, cyp102, cyp106, cyp107, cyp109, cyp134, cyp152, and cyp197. previously, we identified the catalytic activities of three p450s of bacillus subtilis towards steroids by rapid substrate screening using fourier transform ion cyclo ... | 2009 | 19559718 |
| critical illness polyneuropathy after bacillus cereus sepsis in acute lymphoblastic leukemia. | we report a 16-year-old woman patient with acute lymphoblastic leukemia (all) who developed severe critical illness polyneuropathy (cip). she developed bacillus cereus sepsis with multiple brain abscesses after chemotherapy. although she was successfully treated and her general condition recovered, she could not stand. cip was diagnosed based on the clinical course and electrophysiological studies. since vincristine (vcr) was thought to have aggravated neurological symptoms, all treatment was co ... | 2009 | 19571454 |
| nanoparticles affect the survival of bacteria on leaf surfaces. | the increasing presence of nanomaterial and nanoproducts makes it imperative to learn more about the associated impacts of these materials on human health and the environment. in this study, the effect of the nanomaterial tio2 on the phyllosphere microbial community was investigated. analysis results by pcr-denaturing gradient gel electrophoresis revealed a tio2-induced change in the community structure of microorganisms. an epiphytic bacterium, bacillus cereus 905, was chosen to study the role ... | 2009 | 19573199 |
| bacillus cereus as a biotemplating agent for the synthesis of zinc oxide with raspberry- and plate-like structures. | currently the development of green chemistry approach with the use of biomaterial-based activities of microbial cells in the synthesis of various nanostructures has attracted a great attention. in this study, we report on the use of bacterium, bacillus cereus as a biotemplating agent for the formation of zinc oxide nanoparticles with raspberry- and plate-like structures through a simple thermal decomposition of zinc acetate by maintaining the original ph of the reaction mixtures. possible mechan ... | 2009 | 19577306 |
| synergistic effect of high hydrostatic pressure and natural antimicrobials on inactivation kinetics of bacillus cereus in a liquid whole egg and skim milk mixed beverage. | an in-depth study was conducted in order to extend the storage life of a liquid whole egg-skim milk (lwe-sm) mixed beverage to enhance its safety and the safety of related beverages. bacillus cereus vegetative cells (1 x 10(8) colony-forming units [cfu]/ml) were inoculated in lwe-sm beverages with or without natural antimicrobial supplements: flavonol rich-cocoa powder (cocoanox 12%, ccx) (700 ppm), vanillin (700 ppm), anise (700 ppm), and cinnamon (700 ppm). b. cereus cells were maintained at 1 ... | 2009 | 19580454 |
| assessment of the medicinal potentials of the methanol extracts of the leaves and stems of buddleja saligna. | buddleja saligna willd (loganiaceae) is a small to medium-sized evergreen tree; trunk short, often gnarled and crooked; crown dense, rounded or domed-shaped; foliage greyish green. the wild olives are traditionally used to lower blood pressures in many parts of the world. in southern africa, bark and leaf decoctions are used to treat colic, coughs, colds, sore eyes, urinary problems and as purgatives. | 2009 | 19580647 |
| necrotizing fasciitis and myonecrosis "synergistic necrotizing cellulitis" caused by bacillus cereus. | our patient was a 37-year-old man with diabetes mellitus and hepatopathy as underlying diseases. swelling, erythema and pain appeared in the left upper limb on the day before the initial examination. on examination, diffuse purpura was noted on the left upper limb, and, as it rapidly extended to the left upper trunk, emergency surgery was performed. intraoperatively, gas-producing necrosis was observed not only in subcutaneous tissues but also from the fascia to muscle tissues, and the condition ... | 2009 | 19583692 |
| development of a pcr assay for identification of the bacillus cereus group species. | a pcr technique was developed as a reliable and rapid identification method for the bacillus cereus group species, based on a unique conserved sequence of the motb gene (encoding flagellar motor protein) from b. cereus, bacillus thuringiensis and bacillus anthracis. | 2010 | 19583794 |
| superdormant spores of bacillus species have elevated wet-heat resistance and temperature requirements for heat activation. | purified superdormant spores of bacillus cereus, b. megaterium, and b. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations, thus allowing isolation of the superdormant spores, and even with alternate germinants. however, these superdormant spores exhibited significant germination with the original or a ... | 2009 | 19592590 |
| an oxidant- and solvent-stable protease produced by bacillus cereus sv1: application in the deproteinization of shrimp wastes and as a laundry detergent additive. | the current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. in this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by bacillus cereus sv1. maximum protease activity (5,900 u/ml) was obtained when the strain was grown in medium containing 40 g/l shrimp wastes powder as a sole carbon source. the optimum ph, optimum temperature, ph stability, and thermal stability of ... | 2010 | 19593670 |
| survival of foodborne pathogens in grain products and the effect of catechins. | we investigated the survival of bacterial pathogens in grain products. salmonella enteritidis and staphylococcus aureus survived for more than 3 weeks in grain flakes. although s. enteritidis in grain flakes reached a level of less than 10(2) cfu/g, the surviving organisms grew rapidly in milk and reached 10(9) cfu/g after 25-hr incubation. when catechins were present on the grain flakes, the numbers of s. enteritidis and s. aureus were significantly decreased. in addition, catechins reduced the ... | 2009 | 19602859 |
| broad range evaluation of the matrix solubilization (matrix lysis) strategy for direct enumeration of foodborne pathogens by nucleic acids technologies. | a previously published rapid (<5 h) proof-of-concept protocol for the concentration of the foodborne pathogen listeria monocytogenes from milk, based on the solubilization of the food matrix, was further evaluated. the original protocol was modified to detect gram-negative and other gram-positive bacteria and to broaden the range of food matrices by using lutensol instead of sodium dodecyl sulfate as the main detergent in the buffer. a new protocol using a protease and sucrose buffer was establi ... | 2009 | 19610333 |
| simultaneous identification of seven foodborne pathogens and escherichia coli (pathogenic and nonpathogenic) using capillary electrophoresis-based single-strand conformation polymorphism coupled with multiplex pcr. | the objective of this study was to develop a novel technique for parallel analysis of eight important foodborne microbes using capillary electrophoresis-based single-strand conformation polymorphism (ce-sscp) coupled with multiplex pcr. specific primers for multiplex pcr amplification of the 16s rrna gene were designed, corresponding to eight species of bacteria, including escherichia coli, clostridium perfringens, campylobacter jejuni, salmonella enterica, listeria monocytogenes, vibrio parahae ... | 2009 | 19610337 |
| a response surface model to describe the effect of temperature and ph on the growth of bacillus cereus in cooked rice. | this study was performed to develop a predictive model for the growth rate of bacillus cereus in cooked rice. a response surface methodology (rsm) was used with a combination of storage temperature (10 to 40 degrees c) and ph value (5.4 to 6.8). the growth curves generated under different conditions were fitted using a modified gompertz equation, and the relationship of the growth rate to the growth curves was modeled using an rsm quadratic polynomial equation. the predictive model was significa ... | 2009 | 19610344 |
| bacillus cereus in refrigerated milk submitted to different heat treatments. | the possibility of the survival, germination, and multiplication of bacillus cereus in extended-shelf-life milk prompted research into the occurrence of the bacteria in refrigerated milk submitted to different heat treatments. samples were submitted to ultrapasteurization (138 degrees c for 2 s), "superpasteurization" (96 degrees c for 13 s), and pasteurization (74 degrees c for 15 s) and stored under refrigeration at 4 +/- 2 degrees c for up to 6 weeks. the milk was analyzed for its sensory qua ... | 2009 | 19610345 |
| synthesis, characterization and evaluation of antibacterial activity of some thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties. | a series of thiazolo[3,2-b][1,2,4]triazole incorporating diphenylsulfone moieties were synthesized starting from 5-[4-(4-x-phenylsulfonyl)phenyl]-4h-1,2,4-triazole-3-thioles 3a-c, x=h, cl, br. thus, alkylation of 1,2,4-triazoles 3 with phenacyl bromide or 4-bromophenacyl bromide afforded s-substituted 1,2,4-triazoles 4, 5. these new intermediates 4 and 5, in the presence of h(2)so(4) (c), were cyclized to 2-[4-(4-x-phenylsulfonyl)phenyl]-6-(4-y-phenyl)[1,3]thiazolo[3,2-b]-[1,2,4]-triazoles 6, 7 ... | 2009 | 19616347 |
| the small acid soluble proteins (sasp alpha and sasp beta) of bacillus weihenstephanensis and bacillus mycoides group 2 are the most distinct among the bacillus cereus group. | the bacillus cereus group includes bacillus anthracis, b. cereus, bacillus thuringiensis, bacillus mycoides and bacillus weihenstephanensis. the small acid soluble spore protein (sasp) beta has been previously demonstrated to be among the biomarkers differentiating b. anthracis and b. cereus; sasp beta of b. cereus most commonly exhibits one or two amino acid substitutions when compared to b. anthracis. sasp alpha is conserved in sequence among these two species. neither sasp alpha nor beta for ... | 2009 | 19616612 |
| antimicrobial, mechanical, and barrier properties of cassava starch-chitosan films incorporated with oregano essential oil. | the physicochemical and antimicrobial properties of starch-chitosan films incorporated with oregano essential oil (oeo) have been investigated. the antimicrobial effects of starch-chitosan-oeo films against bacillus cereus, escherichia coli, salmonella enteritidis, and staphylococcus aureus were determined by the disk inhibition zone method. the film mechanical properties, water vapor permeability (wvp), fourier transform infrared spectra (ftir), and thermograms (tga) were also determined. films ... | 2009 | 19627142 |
| differentiation of whole bacterial cells based on high-throughput microarray chip printing and infrared microspectroscopic readout. | using robotic automation, a microarray printing protocol for whole bacterial cells was developed for subsequent label-free and nondestructive infrared microspectroscopic detection. using this contact microspotting system, 24 microorganisms were printed on zinc selenide slides; these were 6 species of listeria, 10 species of vibrio, 2 strains of photobacterium damselae, yersinia enterocolitica 289, bacillus cereus atcc 14529, staphylococcus aureus, atcc 19075 (serotype 104 b), shigella sonnei 201 ... | 2009 | 19630511 |
| literature review on the safety of toyocerin, a non-toxigenic and non-pathogenic bacillus cereus var. toyoi preparation. | bacillus cereus var. toyoi is a naturally occurring, non-toxigenic and non-pathogenic strain of b. cereus. safety studies were conducted on a b. toyoi preparation (toyocerin, including but not limited to enterotoxicity, eye irritation, genotoxicity, acute, subchronic and chronic toxicity studies and human clinical trials. in rabbits, toyocerin did not exhibit enterotoxicity and was only slightly irritating to the eyes. it was non-mutagenic in an ames assay at up to 10,000 microg/plate and did no ... | 2009 | 19631708 |
| kefiran protects caco-2 cells from cytopathic effects induced by bacillus cereus infection. | the aim of this work was to evaluate the ability of kefiran to antagonize cytopathic effects triggered by bacillus cereus strain b10502 on cultured human enterocytes (caco-2 cells). cell damage was evaluated by f-actin labelling, scanning electron microscopy and determination of ratios of necrotic and detached cells. to assess the interaction between kefiran and bacteria or eukaryotic cells, flow cytometric analysis was conducted with fitc-labelled kefiran. kefiran significantly protected infect ... | 2009 | 19633916 |
| seasonal variation and bioactivity in the leaf oil of liriodendron tulipifera growing in huntsville, alabama. | the seasonal variation in the chemical composition of the leaf essential oil of liriodendron tulipifera has been analyzed by gc-ms. two individual trees were sampled five times during the course of the growing season. twenty components were identified in the leaf oils, which were dominated by sesquiterpene hydrocarbons, principally germacrene d and beta-elemene, in the early part of the season (42-44% and 18-23%, respectively,) but monoterpene hydrocarbons, largely (z)-beta-ocimene, dominated th ... | 2009 | 19634333 |
| bacteriocin production by staphylococcus aureus involved in bovine mastitis in brazil. | in the present study, 257 staphylococcus spp. strains were isolated from bovine mastitis cases in 56 different brazilian dairy herds located in the southeast region of the country and tested for antimicrobial substance (ams) production. forty-six strains (17.9%) exhibited ams production and their identification as staphylococcus aureus was based on the presence of gram-positive cocci and on positive results in tests for the ability to coagulate rabbit plasma, to ferment mannitol, and to produce ... | 2009 | 19635553 |
| bacillus cereus spores release alanine that synergizes with inosine to promote germination. | the first step of the bacterial lifecycle is the germination of bacterial spores into their vegetative form, which requires the presence of specific nutrients. in contrast to closely related bacillus anthracis spores, bacillus cereus spores germinate in the presence of a single germinant, inosine, yet with a significant lag period. | 2009 | 19636427 |
| mechanisms of catalysis and inhibition operative in the arginine deiminase from the human pathogen giardia lamblia. | giardia lamblia arginine deiminase (glad), the topic of this paper, belongs to the hydrolase branch of the guanidine-modifying enzyme superfamily, whose members employ cys-mediated nucleophilic catalysis to promote deimination of l-arginine and its naturally occurring derivatives. g. lamblia is the causative agent in the human disease giardiasis. the results of rnai/antisense rna gene-silencing studies reported herein indicate that glad is essential for g. lamblia trophozoite survival and thus, ... | 2009 | 19640561 |
| detection of enterobacter sakazakii and other pathogens associated with infant formula powder by use of a dna microarray. | pathogen detection is critical to the process of generating and testing powdered infant formula (pif). an obstacle associated with pif microbial surveillance is that most current procedures are time-consuming and labor-intensive. we have developed a rapid, dna microarray-based detection technique to identify 10 different pathogenic bacteria associated with pif contamination based on the 16s-23s rrna gene internal transcribed spacer (its) sequences and wzy (o antigen polymerase) gene. using this ... | 2009 | 19641057 |
| antibacterial compounds from mushrooms i: a lanostane-type triterpene and prenylphenol derivatives from jahnoporus hirtus and albatrellus flettii and their activities against bacillus cereus and enterococcus faecalis. | antibacterial bioassay-guided fractionation of two american mushroom species, jahnoporus hirtus and albatrellus flettii, led to the isolation and identification of their major antibacterial constituents: 3,11-dioxolanosta-8,24( z)-diene-26-oic acid (1) from j. hirtus and confluentin (2), grifolin (3), and neogrifolin (4) from a. flettii. compound 1 is a new lanostane-type triterpene. all purified compounds were evaluated for their ability to inhibit the growth of bacillus cereus and enterococcus ... | 2010 | 19644795 |
| survey of bacterial populations present in us-produced linerboard with high recycle content. | to survey paperboard products from 17 us mills for bacterial populations and for bacteria potentially harmful to human health. | 2010 | 19645765 |
| novel and unique diagnostic biomarkers for bacillus anthracis infection. | a search for bacterium-specific biomarkers in peripheral blood following infection with bacillus anthracis was carried out with rabbits, using a battery of specific antibodies generated by dna vaccination against 10 preselected highly immunogenic bacterial antigens which were identified previously by a genomic/proteomic/serologic screen of the b. anthracis secretome. detection of infection biomarkers in the circulation of infected rabbits could be achieved only after removal of highly abundant s ... | 2009 | 19648366 |
| biofilm formation and cell surface properties among pathogenic and nonpathogenic strains of the bacillus cereus group. | biofilm formation by 102 bacillus cereus and b. thuringiensis strains was determined. strains isolated from soil or involved in digestive tract infections were efficient biofilm formers, whereas strains isolated from other diseases were poor biofilm formers. cell surface hydrophobicity, the presence of an s layer, and adhesion to epithelial cells were also examined. | 2009 | 19648377 |
| [a case of sepsis in ilbw infant caused by bacillus cereus]. | bacillus cereus is an aerobic or facultative anaerobic gram-positive rod which is ubiquitous in the environment. the incidence of neonatal infections is very low. the clinical course is serious, usually life- threatening or permanently damaging the central nervous system. the immature immune system, subjected to invasive procedures, increases the morbidity risk in this age group, especially in extremely low birth weight infants. diagnostic difficulties and insusceptibility to ''first- line'' ant ... | 2009 | 19648658 |
| antimicrobial susceptibility, beta-lactamase and enterotoxin production in bacillus cereus isolates from clinical and food samples. | the antimicrobial susceptibility of 30 clinical and 30 food bacillus cereus isolates was determined. all isolates were susceptible to streptomycin, ciprofloxacin and gentamicin, 90 % of them to clindamycin and vancomycin, and 67 % to erythromycin. all isolates were resistant to amoxicillin with clavulanic acid, ampicillin, cefotaxime, ciprofloxacin, cloxacillin, cefotaxime with clavulanic acid and penicillin. the mic values (determined by e-tests) were 48-256 mg/l for ampicillin, 0.19-1.5 mg/l f ... | 2009 | 19649741 |
| structure and applications of a rhamnolipid surfactant produced in soybean oil waste. | soybean oil soapstock was utilized as an alternative carbon source for the production of rhamnolipids by pseudomonas aeruginosa lbi strain. the chemical composition and properties of the rhamnolipid mixture obtained were determined to define its potential applications. the chemical characterization of the rhamnolipid has revealed the presence of ten different homologues. the monorhamnolipid rhac10c1) and the dirhamnolipid rha2c10c10 were the main components of the mixture that showed predominanc ... | 2010 | 19649781 |
| changes in membrane fatty acids and murein composition of bacillus cereus and salmonella typhi induced by gamma irradiation treatment. | this present study was carried out to evaluate the effects of gamma irradiation on the fatty acids (fa) and mureins composition of two different radiotolerant bacteria. bacillus cereus lspq 2872 and salmonella typhi atcc 19430 were used for this study. the bacterial strains were treated with a sublethal radiation dose of 1kgy to cause a cellular damage. gas chromatography (gc) and high performance liquid chromatography (hplc) analysis were performed to demonstrate respectively the modification o ... | 2009 | 19651456 |
| bacillus anthracis physiology and genetics. | bacillus anthracis is a member of the bacillus cereus group species (also known as the "group 1 bacilli"), a collection of gram-positive spore-forming soil bacteria that are non-fastidious facultative anaerobes with very similar growth characteristics and natural genetic exchange systems. despite their close physiology and genetics, the b. cereus group species exhibit certain species-specific phenotypes, some of which are related to pathogenicity. b. anthracis is the etiologic agent of anthrax. ... | 2009 | 19654018 |
| complete sequence of three plasmids from bacillus thuringiensis inta-fr7-4 environmental isolate and comparison with related plasmids from the bacillus cereus group. | bacillus thuringiensis is an insect pathogen used worldwide as a bioinsecticide. it belongs to the bacillus cereus sensu lato group as well as bacillus anthracis and b. cereus. plasmids from this group of organisms have been implicated in pathogenicity as they carry the genes responsible for different types of diseases that affect mammals and insects. some plasmids, like paw63 and pbt9727, encode a functional conjugation machinery allowing them to be transferred to a recipient cell. they also sh ... | 2009 | 19654019 |
| production and structural characterization of crystalline silver nanoparticles from bacillus cereus isolate. | to date, biosynthesis of metal nanoparticles has been intensively studied using bacteria and fungi. we have isolated and identified metal resistant bacterial strains from electroplating industries, they produce silver nanoparticles. the reduction reaction of aqueous silver nitrate with bacterial biomass was carried out for 120 h. bacteria produced metallic nanoparticles showed a strong absorbance at surface plasmon resonance wavelength around 420 nm. the size and morphology of these nanoparticle ... | 2009 | 19660920 |
| the synthesis of 2- and 3-aryl indoles and 1,3,4,5-tetrahydropyrano[4,3-b]indoles and their antibacterial and antifungal activity. | a series of 2- and 3-aryl substituted indoles and two 1,3,4,5-tetrahydropyrano[4,3-b]indoles were synthesized from indole and 5-methoxyindole. the 2-aryl indoles were synthesized from the 1-(phenylsulfonyl)indole derivatives using magnesiation followed by iodination. the 2-iodinated compounds were then subjected to suzuki-miyaura reactions. in addition, the 3-aryl indoles were made from the corresponding 3-bromoindoles using suzuki-miyaura reactions. the 1,3,4,5-tetrahydropyrano[4,3-b]indoles we ... | 2009 | 19660944 |
| plasma assisted decontamination of bacterial spores. | the efficacy and mechanism of killing bacterial spores by a plasma torch is studied. bacterial-spore (bacillus cereus) suspension is inoculated onto glass/paper slide-coupons and desiccated into dry samples, and inoculated into well-microplate as wet sample. the exposure distance of all samples is 4 cm from the nozzle of the torch. in the experiment, paper slide-coupon is inserted inside an envelope. the kill times on spores in three types of samples are measured to be about 3, 9, and 24 seconds ... | 2008 | 19662115 |
| characterization of lactobacillus spp. isolated from the feces of breast-feeding piglets. | lactobacillus spp., referred to as ij-1 and ij-2, were isolated from the feces of breast-feeding piglets and analyzed for probiotic properties. according to the analyses of 16s rdna sequence, lactobacillus sp. ij-1 showed greater than 99% homology with lactobacillus reuteri dsm 20016(t), and lactobacillus sp. ij-2 had greater than 99% homology with the l. gasseri atcc 33323(t) and l. johnsonii atcc 33200(t). the ph changes in the culture media of lactobacillus sp. ij-1 and lactobacillus sp. ij-2 ... | 2009 | 19664551 |
| positively cooperative binding of zinc ions to bacillus cereus 569/h/9 beta-lactamase ii suggests that the binuclear enzyme is the only relevant form for catalysis. | metallo-beta-lactamases catalyze the hydrolysis of most beta-lactam antibiotics and hence represent a major clinical concern. while enzymes belonging to subclass b1 have been shown to display maximum activity as dizinc species, the actual metal-to-protein stoichiometry and the affinity for zinc are not clear. we have further investigated the process of metal binding to the beta-lactamase ii from bacillus cereus 569/h/9 (known as bcii). zinc binding was monitored using complementary biophysical t ... | 2009 | 19665032 |
| development of a real-time pcr assay for detection and quantification of enterotoxigenic members of bacillus cereus group in food samples. | a highly sensitive real-time pcr (qpcr) procedure, targeting the phosphatidylcholine-specific phospholipase c gene (pc-plc), was developed for specific detection and quantification of strains belonging to bacillus cereus group. the target region was selected based on the enterotoxigenic profiles of 75 bacillus strains. the inclusivity and exclusivity of the rti-pcr assay were assessed with 59 isolates of the b. cereus group, 16 other bacillus spp., and 4 non-bacillus strains. the assay was also ... | 2009 | 19665814 |
| characterization of sucrose-glutamic acid maillard products (sgmps) degrading bacteria and their metabolites. | two aerobic bacteria rnbs1 and rnbs3 capable to degrade and utilize sucrose-glutamic acid maillard products (sgmps) as carbon, nitrogen and energy source were isolated and characterized as alcaligenes faecalis (dq659619) and bacillus cereus (dq659620) respectively by 16s rrna gene sequence analysis. in present study, mixed bacterial culture was found more effective compared to axenic culture rnbs1 and rnbs3 decolourizing 73.79%, 66.80% and 62.56% sgmps, respectively. the sgmps catabolizing enzym ... | 2009 | 19665891 |
| the cca anticodon specifies separate functions inside and outside translation in bacillus cereus. | bacillus cereus 14579 encodes two trnas with the cca anticodon, trna(trp) and trna(other). trna(trp) was separately aminoacylated by two enzymes, trprs1 and trprs2, which share only 34% similarity and display different catalytic capacities and specificities. trprs1 was 18-fold more proficient at aminoacylating trna(trp) with trp, while trprs2 more efficiently utilizes the trp analog 5-hydroxy trp. trna(other) was not aminoacylated by either trprs but instead by the combined activity of lysrs1 an ... | 2009 | 19667754 |
| antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens. | crude extracts were prepared using the solvent ethanol from the seaweeds viz., gracilaria edulis, calorpha peltada and hydroclothres sp. and screening for their antibacterial activity against six bacterial pathogens were also carried out. the test bacterial strains were escherichia coli, enterobacter aerogenes, staphylococcus aureus, pseudomonas aeruginosa, streptococcus faecalis and bacillus cereus. ethanol extract of gracilaria edulis inhibited growth of all the test organisms except bacillus ... | 2009 | 19673167 |
| the preparation, germination properties and stability of superdormant spores of bacillus cereus. | to determine yields, germination and stability of superdormant bacillus cereus spores. | 2010 | 19674187 |
| growth and gaseous emissions of pure and mixed small intestinal bacterial cultures: effects of bile and vancomycin. | simultaneous cultivations in anaerobiosis, aerobiosis and with microaerobic gas mixture were used to clarify the bile (oxgall) effects on the pure and mixed cultures of enterobacterial strains in simulations in portable microbe enrichment unit (pmeu) linked with chempro100i((r)) gas detector. the effects of vancomycin were evaluated in aerobic cultures. growth and metabolic activity of cultures were also followed by measuring sugar consumption, ph alterations, and colony counts on bd chromagar o ... | 2010 | 19682874 |
| inhibition of nitrate reduction by chromium (vi) in anaerobic soil microcosms. | chromium is often found as a cocontaminant at sites polluted with organic compounds. for nitrate-respiring microbes, cr(vi) may be not only directly toxic but may also specifically interfere with n reduction. in soil microcosms amended with organic electron donors, cr(vi), and nitrate, bacteria oxidized added carbon, but relatively low doses of cr(vi) caused a lag and then lower rates of co(2) accumulation. cr(vi) strongly inhibited nitrate reduction; it occurred only after soluble cr(vi) could ... | 2009 | 19684175 |
| expression of bacillus cereus hemolysin ii in bacillus subtilis renders the bacteria pathogenic for the crustacean daphnia magna. | hemolysin ii (hlyii) is a pore-forming toxin of the opportunistic pathogen bacillus cereus. despite our understanding of the mechanism of hlyii cytotoxicity in vitro, many of its characteristics, including potential target cells, conditions of its action and expression, are not known. here we report that the expression of hlyii in bacillus subtilis renders the bacteria hemolytic and is able to kill the crustacean daphnia magna. the hemolytic activity of hlyii-encoded b. subtilis strains in cultu ... | 2009 | 19686345 |
| characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (lepidoptera: noctuidae). | the characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (anticarsia gemmatalis) were the objectives of this study. twelve aerobic and anaerobic isolates of proteolytic bacteria were obtained from the caterpillar gut in calcium caseinate agar. the number of colony forming units (cfus) of proteolytic bacteria was higher when the bacteria were extracted from caterpillars reared on artificial diet rather than on soybean leaves (1.73 +/- 0.35 x 10(3) ... | 2009 | 19689886 |
| a noninvasive near infrared system for detection of platelet components contaminated with bacteria. | platelet (plt) transfusion-associated bacterial sepsis has remained a substantial patient risk, primarily due to lacking effective and point-of-issue measures to detect bacterial contamination. this study describes near infrared (nir) spectroscopy to examine inoculated plts without sampling within a few seconds. | 2010 | 19694992 |
| treatment and outcome of traumatic endophthalmitis in open globe injury with retained intraocular foreign body. | our aim was to evaluate the incidence, risk factors, pathogens, use of systemic and intravitreal antibiotics, and outcome of traumatic endophthalmitis after open globe injury with retained intraocular foreign body (riofb). | 2010 | 19707031 |
| production of bioactive metabolites by nocardia levis mk-vl_113. | to isolate and identify the bioactive compounds produced by nocardia levis mk-vl_113. | 2009 | 19708882 |
| differential transfer dynamics of paw63 plasmid among members of the bacillus cereus group in food microcosms. | to assess the dynamics of plasmid transfer between bacillus thuringiensis and b. cereus in various food microcosms using the b. thuringiensis paw63 and staphylococcus aureus pub110 plasmids as models. | 2010 | 19709333 |
| effect of mixed antimicrobial agents and flavors in active packaging films. | active packaging is an emerging food technology to improve the quality and safety of food products. many works have been developed to study the antimicrobial activity of essential oils. essential oils have been traditionally used as flavorings in food, so they have an important odor impact but they have as well antimicrobial properties that could be used to protect the food. recent developments in antimicrobial active packaging showed the efficiency of essential oils versus bread and bakery prod ... | 2009 | 19711918 |
| an extracytoplasmic function sigma factor controls beta-lactamase gene expression in bacillus anthracis and other bacillus cereus group species. | the susceptibility of most bacillus anthracis strains to beta-lactam antibiotics is intriguing considering that the closely related species bacillus cereus and bacillus thuringiensis typically produce beta-lactamases and the b. anthracis genome harbors two beta-lactamase genes, bla1 and bla2. we show that beta-lactamase activity associated with b. anthracis is affected by two genes, sigp (ba2502) and rsip (ba2503), predicted to encode an extracytoplasmic function sigma factor and an anti-sigma f ... | 2009 | 19717606 |
| microbial succession in the rhizosphere of live and decomposing barley roots as affected by the antagonistic strain pseudomonas fluorescens dr54-bn14 or the fungicide imazalil. | abstract the protocol used in the present study was a long-term mesocosm experiment where the microbial succession around live barley roots and subsequent decomposing roots was assessed after seed coating with either the antagonistic strain pseudomonas fluorescens dr54-bn14 or the fungicide imazalil. four diversity measures were used: community level physiological profiles (clpp), bacteria-specific polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge), actinomycete-specifi ... | 2003 | 19719670 |
| sources of bacillus cereus contamination in a pasteurized zucchini purée processing line, differentiated by two pcr-based methods. | in previous work, raw materials used for processing pasteurized zucchini purée (zucchini, milk proteins and starch) and cultivation soil of zucchinis were found to be potential sources of stored product contamination with bacillus cereus. 134 b. cereus strains originating from these sources and from the stored product were typed using coliphage m13 sequence-based polymerase chain reaction (m13-pcr) and dna amplification fingerprinting. combined patterns from the two methods were compared using d ... | 2003 | 19719681 |
| assay of enterocin as-48 for inhibition of foodborne pathogens in desserts. | enterocin as-48 was tested against staphylococcus aureus, bacillus cereus, and listeria monocytogenes in different kinds of desserts. the highest activity against s. aureus was detected in baker cream. however, in yogurt-type soy-based desserts and in gelatin pudding, as-48 (175 arbitrary units [au]/g) reduced viable cell counts of s. aureus by only 1.5 to 1.8 log units at most. the efficacy of as-48 in puddings greatly depended on inoculum size, and viable s. aureus counts decreased below detec ... | 2009 | 19722396 |
| the genome and variation of bacillus anthracis. | the bacillus anthracis genome reflects its close genetic ties to bacillus cereus and bacillus thuringiensis but has been shaped by its own unique biology and evolutionary forces. the genome is comprised of a chromosome and two large virulence plasmids, pxo1 and pxo2. the chromosome is mostly co-linear among b. anthracis strains and even with the closest near neighbor strains. an exception to this pattern has been observed in a large inversion in an attenuated strain suggesting that chromosome co ... | 2009 | 19729033 |
| new approach for the detection of non-ribosomal peptide synthetase genes in bacillus strains by polymerase chain reaction. | bacillus strains produce non-ribosomal lipopeptides that can be grouped into three families: surfactins or lichenysins, iturins and fengycins or plispastatins. these biosurfactants show a broad spectrum of biological activities. to detect strains able to produce these lipopeptides, a new polymerase chain reaction screening approach was developed using degenerated primers based on the intraoperon alignment of adenylation and thiolation nucleic acid domains of all enzymes implicated in the biosynt ... | 2010 | 19730852 |
| volatile constituents and antibacterial screening of the essential oil of chenopodium ambrosioides l. growing in nigeria. | the essential oil of the aerial parts of chenopodium ambrosioides l. has been isolated by hydrodistillation and analyzed using gc-ms. the major components were found to be alpha-terpinene (63.1%), p-cymene (26.4%) and ascaridole (3.9%). the oil displayed no antibacterial activity against either gram-positive bacteria bacillus cereus or staphylococcus aureus, or the gram-negative bacterium escherichia coli (mic=1250 microg/ml). a cluster analysis of c. ambrosioides essential oils reveals at least ... | 2009 | 19731609 |
| biosynthesis and transcriptional analysis of thurincin h, a tandem repeated bacteriocin genetic locus, produced by bacillus thuringiensis sf361. | thurincin h, a bacteriocin produced by bacillus thuringiensis sf361 isolated from honey, strongly inhibited the growth of bacillus cereus f4552. the bacteriocin was purified by 65% ammonium sulfate precipitation of the culture supernatant, followed by octyl-sepharose cl-4b and reverse-phase hplc. the molecular mass of the bacteriocin was determined to be 3139.51 da and the 14 amino acids of the bacteriocin at the n-terminus were identified. the complete amino acid sequence of mature thurincin h ... | 2009 | 19732155 |
| in vitro antimicrobial effect of satureja wiedemanniana against bacillus species isolated from raw meat samples. | in this study a total of 30 raw meat samples obtained from ankara, turkey were screened for the presence of bacillus species. among the meat samples analyzed, the predominant species isolated was bacillus circulans; other bacillus species were identified as bacillus firmus, bacillus lentus, bacillus megaterium, bacillus licheniformis, bacillus mycoides, bacillus sphaericus, and bacillus cereus. minced meat samples were more contaminated with bacillus species than sliced beef sample. from these s ... | 2009 | 19735196 |
| microbial toxin's effect on mitochondrial survival by increasing k+ uptake. | we studied the effects of toxins, which inhibited the motility of boar spermatozoa, on rat liver mitochondria. the toxins studied were originally from bacteria isolated from moisture-damaged buildings where inhabitants exhibited symptoms, or from food causing poisoning. some strains of bacillus cereus and streptomyces griseus produced potassium ionophoric peptides cereulide and valinomycin (mikkola, et al., european journal of biochemistry 1999; 263: 112-117). of interest is that channels were f ... | 2009 | 19736254 |
| antibiotic resistance in bacteria: novel metalloenzyme inhibitors. | beta-lactam antibiotics are among the most important drugs used to fight bacterial infection. overuse and misuse of beta-lactam antibiotics has caused the evolution of resistance mechanisms, allowing pathogenic bacteria to survive antibiotic treatment. the major source of resistance to beta-lactam antibiotics occurs through production of enzymes called beta-lactamases capable of catalyzing hydrolysis of the beta-lactam rings in these drug compounds. the metallo-beta-lactamases have become a majo ... | 2009 | 19751419 |
| enteropathogenicity of bacillus cereus isolated from some nigerian foods. | bacillus cereus is a soil bacterium and has been isolated from cooked and uncooked rice, milk and dairy products, meat, spices, garlic, vegetables, cereals and infant formulae. | 2009 | 19761179 |
| evaluation of antimicrobial peptides as novel bactericidal agents for room temperature-stored platelets. | a single cost-effective pathogen inactivation approach would help to improve the safety of our nation's blood supply. several methods and technologies are currently being studied to help reduce bacterial contamination of blood components. there is clearly need for simple and easy-to-use pathogen inactivation techniques specific to plasma, platelets (plts), and red blood cells. | 2010 | 19761549 |
| the impact of oxygen availability on stress survival and radical formation of bacillus cereus. | both the growth and stress survival of two model bacillus cereus strains, atcc 14579 and atcc 10987, were tested in three different conditions varying in oxygen availability, i.e., aerobic, microaerobic and anaerobic conditions. both b. cereus strains displayed highest growth rates and yields under aerobic conditions, whereas the microaerobic and anaerobic cultures showed similar reduced growth performances. the cells grown and exposed microaerobically and anaerobically were more resistant to he ... | 2009 | 19762101 |
| effect of bile salts on the dna and membrane integrity of enteric bacteria. | enteric bacteria are able to resist the high concentrations of bile encountered throughout the gastrointestinal tract. here we review the current mechanisms identified in the enteric bacteria salmonella, escherichia coli, bacillus cereus and listeria monocytogenes to resist the dangerous effects of bile. we describe the role of membrane transport systems, and their connection with dna repair pathways, in conferring bile resistance to these enterics. we discuss the findings from recent investigat ... | 2009 | 19762477 |
| the dlt operon of bacillus cereus is required for resistance to cationic antimicrobial peptides and for virulence in insects. | the dlt operon encodes proteins that alanylate teichoic acids, the major components of cell walls of gram-positive bacteria. this generates a net positive charge on bacterial cell walls, repulsing positively charged molecules and conferring resistance to animal and human cationic antimicrobial peptides (amps) in gram-positive pathogenic bacteria. amps damage the bacterial membrane and are the most effective components of the humoral immune response against bacteria. we investigated the role of t ... | 2009 | 19767427 |
| identification and classification of bcl genes and proteins of bacillus cereus group organisms and their application in bacillus anthracis detection and fingerprinting. | the bacillus cereus group includes three closely related species, b. anthracis, b. cereus, and b. thuringiensis, which form a highly homogeneous subdivision of the genus bacillus. one of these species, b. anthracis, has been identified as one of the most probable bacterial biowarfare agents. here, we evaluate the sequence and length polymorphisms of the bacillus collagen-like protein bcl genes as a basis for b. anthracis detection and fingerprinting. five genes, designated bcla to bcle, are pres ... | 2009 | 19767469 |
| identification of bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks. | this report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, bacillus anthracis. bacillus cultures were prepared under standardized conditions and inactivated according to a recently developed ms-compatible inactivation protocol for highly pathogenic microorganisms. maldi-tof ms was then emplo ... | 2009 | 19767470 |
| examination of electron stains as a substitute for uranyl acetate for the ultrathin sections of bacterial cells. | electron staining reagents were examined to find a possible substitute for uranyl acetate (ua) in electron microscopy of bacterial ultrathin sections. four kinds of stains, platinum blue (pt-blue), oolong tea extract (ote), potassium permanganate (kmno(4)) and phosphotungstic acid (pta), were examined in comparison with ua either with or without post-staining with lead citrate (pb). electron microscopy was performed on sections from spurr-embedded cells of a gram-positive bacterium, bacillus cer ... | 2010 | 19767626 |
| identification of a polymorphic collagen-like protein in the crustacean bacteria pasteuria ramosa. | pasteuria ramosa is a spore-forming bacterium that infects daphnia species. previous results demonstrated a high specificity of host clone/parasite genotype interactions. surface proteins of bacteria often play an important role in attachment to host cells prior to infection. we analyzed surface proteins of p. ramosa spores by two-dimensional gel electrophoresis. for the first time, we prove that two isolates selected for their differences in infectivity reveal few but clear-cut differences in p ... | 2009 | 19770039 |
| characterization of single heat-activated bacillus spores using laser tweezers raman spectroscopy. | heat activation of dormant bacterial spores is a short treatment at a sublethal temperature that potentiates and synchronizes spore germination. in this paper, laser tweezers raman spectroscopy (ltrs) was used to study the heat activation of single spores of bacillus cereus and bacillus subtilis. we measured the raman spectra of single spores without treatment, during heat activation at 65 degrees c (b. cereus) or 70 degrees c (b. subtilis), and following heat activation and cooling to 25 degree ... | 2009 | 19770863 |
| inhibition of salmonella enteritidis by cerein 8a, edta and sodium lactate. | the ability of the bacteriocin cerein 8a to inhibit salmonella enteritidis in combination with edta and sodium lactate was investigated. salmonella enteritidis was incubated with combinations of cerein 8a (3200au/ml) and edta (20, 50, 100 mmol/l) or sodium lactate (200 mmol/l). all treatments caused a significant reduction in the od(600) values of salmonella enteritidis cultures. the addition of cerein 8a plus edta resulted in higher inhibition in comparison with the bacteriocin alone; the great ... | 2009 | 19775768 |
| study on the antibiotic activity of microcapsule curcumin against foodborne pathogens. | curcumin as an edible natural pigment has several biological activities but its use as a food colorant and preservative is restricted due to low stability and insolubility in water. stabilizing it with microencapsulation to obtain microcapsule curcumin can improve its stability and solubility. the microcapsule curcumin was studied for their antibacterial and antifungal activities against some foodborne pathogens and spoilage microbe such as escherichia coli, yersinia enterocolitica, staphylococc ... | 2009 | 19775769 |
| expression of recombinant antibacterial lactoferricin-related peptides from pichia pastoris expression system. | four recombinant antimicrobial peptide (ramp) cdnas, constructed from two goat lactoferricin-related peptide cdnas (glfcin and glfcin ii) with/without (his)(6)-tag, were cloned into ppiczalphac and transformed into pichia pastoris smd1168h. after methanol induction, these ramps were expressed and secreted into broth. they were purified after cm-sepharose (without his-tg), histrap (with his-tg) and sephadex g-25 chromatographies. the yield of purified ramp was 0.15 mg/ml of broth. these 4 ramps w ... | 2009 | 19780544 |
| sympatric soil communities of bacillus cereus sensu lato: population structure and potential plasmid dynamics of pxo1- and pxo2-like elements. | eighty soil-borne bacillus cereus group isolates were collected from two neighbouring geographical sites in belgium. their genetic relationships and population structure were assessed using multilocus sequence typing analysis of five chromosomal genes, while the contribution of extrachromosomal elements to the population dynamics was gauged by the presence, diversity and transfer capacity of pxo1- and pxo2-like plasmids. globally, the bacterial population displayed a broad diversity, including a ... | 2009 | 19780824 |
| effects of moderately high pressure plus heat on the germination and inactivation of bacillus cereus spores lacking proteins involved in germination. | to determine the germination and inactivation of bacillus cereus spores lacking various germination proteins using moderately high pressure (mhp) and heat. | 2009 | 19780952 |
| antioxidant, anti-microbial and antimutagenicity activities of pistachio (pistachia vera) green hull extract. | antioxidant, anti-microbial and antimutagenicity activities of pistachio (ahmadaghaei variety) green hull extracts (crude and purified extracts) were studied. at first, different solvents were compared for determining of the best solvent for extraction of phenolic compounds from pistachio green hull. water and acetonitrile with 49.32 and 6.22 (mg of gallic acid equivalents/g sample) were the best and the worst solvent in the extraction of phenolic compounds, respectively. the antioxidant capacit ... | 2010 | 19781589 |
| valinomycin biosynthetic gene cluster in streptomyces: conservation, ecology and evolution. | many streptomyces strains are known to produce valinomycin (vlm) antibiotic and the vlm biosynthetic gene cluster (vlm) has been characterized in two independent isolates. here we report the phylogenetic relationships of these strains using both parsimony and likelihood methods, and discuss whether the vlm gene cluster shows evidence of horizontal transmission common in natural product biosynthetic genes. eight streptomyces strains from around the world were obtained and sequenced for three regi ... | 2009 | 19787052 |
| synthesis of ethyl acetate employing celite-immobilized lipase of bacillus cereus mtcc 8372. | a wide range of fatty acid esters can be synthesized by esterification and transesterification reactions catalyzed by lipases in non-aqueous systems. in the present study, immobilization of a purified alkaline extra-cellular lipase of bacillus cereus mtcc 8372 by adsorption on diatomaceous earth (celite) for synthesis of ethyl acetate via transesterification route was investigated. b. cereus lipase was deposited on celite (77% protein binding efficiency) by direct binding from aqueous solution. ... | 2009 | 19789138 |
| acute encephalopathy of bacillus cereus mimicking reye syndrome. | we present an 11-year-old boy diagnosed as having acute encephalopathy and liver failure with the underlying condition of a metabolic dysfunction. he developed convulsions and severe consciousness disturbance following gastroenteritis after the ingestion of some fried rice. he showed excessive elevation of transaminases, non-ketotic hypoglycemia and hyperammonemia, which were presumed to reflect a metabolic dysfunction of the mitochondrial beta-oxidation, and he exhibited severe brain edema thro ... | 2010 | 19796886 |
| [structure and function of sphingomyelinase]. | bacillus cereus is one that causes of opportunistic human infections. sphingomyelinase produced by b. cereus is assumed a virulence factor for the infection. sphingomyelinase from bacillus cereus (bc-smase) is mg(2+)-containing metalloenzyme. bc-smase is a family of neutral smase (nsmase) and mimics the actions of the endogenous mammalian nsmase in causing differentiation, development, and apoptosis. bc-smase may be a good model for the poorly characterized mammalian nsmase. activation of bc-sma ... | 2009 | 19797879 |
| activity assay for nisin-like acidic bacteriocins using an optimal ph-conditioned gel matrix. | a new zymography for detecting nisin-like acidic bacteriocins was developed using a tricine-sodium dodecyl sulfate (sds) gel and an acidic gel matrix (ph 4.0). after electrophoresis, proteins in the tricine gel were electrotransferred to an optimal ph-conditioned gel matrix (op-cgm). the op-cgm was overlaid with indicator cells (bacillus cereus) embedded in nutrient broth soft agar (0.8%, w/v). antibacterial activity shown as a growth inhibition using b. cereus was detected at approximately 3.8k ... | 2010 | 19799850 |
| comparative analysis of the virulence of invertebrate and mammalian pathogenic bacteria in the oral insect infection model galleria mellonella. | infection of galleria mellonella by feeding a mixture of bacillus thuringiensis spores or vegetative bacteria in association with the toxin cry1c results in high levels of larval mortality. under these conditions the toxin or bacteria have minimal effects on the larva when inoculated separately. in order to evaluate whether g. mellonella can function as an oral infection model for human and entomo-bacterial pathogens, we tested strains of bacillus cereus, bacillus anthracis, enterococcus faecali ... | 2010 | 19800349 |
| ceftazidime, a pseudomonas-active cephalosporin: in-vitro antimicrobial activity evaluation including recommendations for disc diffusion susceptibility tests. | the in-vitro qualities of ceftazidime, as compared to other beta-lactams and three other antimicrobials (amikacin, gentamicin and chloramphenicol), were evaluated in a multilaboratory, multiphasic study in the united states. a total of 12,986 recent clinical isolates were tested by reference dilution methods in six medical centres over 45-60 days. ceftazidime was superior to the comparison cephalosporin (cefamandole) and comparable in spectrum and activity to gentamicin against enterobacteriacea ... | 1981 | 19802985 |