Publications

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in vitro activity of telavancin against clinically important gram-positive pathogens from 69 u.s. medical centers (2015): potency analysis by u.s. census divisions.a total of 8,072 gram-positive isolates collected from 69 medical centers among all 9 u.s. census divisions during the 2015 sentry antimicrobial surveillance program were included. telavancin had minimal inhibitory concentration (mic)50 and mic90 values of 0.03/0.06 μg/ml, respectively, against methicillin-susceptible (mssa) and methicillin-resistant staphylococcus aureus (mrsa). similar activity was also observed among coagulase-negative staphylococci (mic50/90, 0.03/0.06 μg/ml; 100.0% inhibite ...201728727951
telavancin activity in vitro tested against a worldwide collection of gram-positive clinical isolates (2014).telavancin activity was measured against gram-positive pathogens collected worldwide during 2014 using the revised broth microdilution testing method. the results were compared with previous reports.201728735051
safety evaluation of enterocin producer enterococcus sp. strains isolated from traditional turkish cheeses.the purpose of this study was to determine the antimicrobial activity and occurrence of bacteriocin structural genes in enterococcus spp. isolated from different cheeses and also investigate some of their virulence factors. enterococcus strains were isolated from 33 different cheeses. enterococcus faecium (6 strains) and enterococcus faecalis (5 strains) enterocin-producing strains were identified by 16s rdna analyses. structural genes enta, entb, entp and entx were detected in some isolates. mu ...201728735309
recovery of metallo-tolerant and antibiotic resistant psychrophilic bacteria from siachen glacier, pakistan.cultureable bacterial diversity of previously unexplored siachen glacier, pakistan, was studied. out of 50 isolates 33 (66%) were gram negative and 17 (34%) gram positive. about half of the isolates were pigment producers and were able to grow at 4-37°c. 16s rrna gene sequences revealed gram negative bacteria dominated by proteobacteria (especially γ-proteobacteria and β-proteobacteria) and flavobacteria. the genus pseudomonas (51.51%, 17) was dominant among γ- proteobacteria. β-proteobacteria c ...201728746396
first description in europe of emergence of enterococcus faecium st117, carrying both vana/vanb genes, isolated in greece.an enterococcus faecium, carrying both vana/vanb genes was recovered from a patient with bacteraemia, treated in a greek hospital. since this is the first description in europe of e. faecium, carrying both vana/vanb genes, the isolate was further studied.201728754459
clonal dissemination of vancomycin-resistant enterococcus faecium st412 in a brazilian region.vancomycin-resistant enterococcus faecium (vrefm) has emerged as an important global nosocomial pathogen, and this trend is associated with the spread of high-risk clones. here, we determined the genetic and phenotypic features of 93 vrefm isolates that were obtained from patients in 13 hospitals in vitória, espírito santo, brazil, during 2012-2013. all the isolates were vancomycin-resistant and harbored the vana gene. only 6 (6.5%) of the vrefm isolates showed the ability to form biofilm. the 9 ...201728759747
vancomycin-resistant enterococci with vana gene in treated municipal wastewater and their association with human hospital strains.vancomycin-resistant enterococci (vre) are pathogens of increasing medical importance. in brno, czech republic, we collected 37 samples from the effluent of a wastewater treatment plant (wwtp), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the wwtp. samples were cultured selectively. enterococci were identified using maldi-tof ms, phenotypically tested for ...201728763660
revisiting nitrofurantoin for vancomycin resistant enterococci.enterococcal infection has emerged as a major therapeutic challenge. emergence of high level aminoglycoside resistance (hlar) and vancomycin-resistant enterococcus (vre) has further limited the drug therapy in enterococcal infections. however, nitrofuratoin being an old drug reported to have less resistance in comparison to the other classes of antimicrobial agents.201728764160
synthesis and structural characterization of silver nanoparticles stabilized with 3-mercapto-1-propansulfonate and 1-thioglucose mixed thiols for antibacterial applications.the synthesis, characterization and assessment of the antibacterial properties of hydrophilic silver nanoparticles (agnps) were investigated with the aim to probe their suitability for innovative applications in the field of nanobiotechnology. first, silver nanoparticles were synthetized and functionalized with two capping agents, namely 3-mercapto-1-propansulfonate (3mps) and 1-β-thio-d-glucose (tg). the investigation of the structural and electronic properties of the nano-systems was carried o ...201628774148
treatment of vancomycin-resistant enterococci: focus on daptomycin.vancomycin-resistant enterococci (vre) infections are problematic due to limited availability of anti-vre agents and their potential for adverse effects and drug interactions. this review focuses on the role of daptomycin in treating vre infections by summarizing key points of relevant clinical studies.201728779348
clinical and microbiological characteristics of pantoea agglomerans infection in children.pantoea agglomerans is an environmental gram-negative bacterium that rarely is responsible for the infections in humans but it is often a causative factor of a number of occupational diseases. this study evaluated the clinical and microbiological characteristics and pathogenicity of p. agglomerans in children. we retrospectively reviewed microbiological test results for all children (1 month old to 18 years old) who were admitted to our pediatric hospital between january 2000 to june 2015 and ha ...201728780309
inhibitory substances production by lactobacillus plantarum st16pa cultured in hydrolyzed cheese whey supplemented with soybean flour and their antimicrobial efficiency as biopreservatives on fresh chicken meat.cheese whey, the main byproduct of the dairy industry, is one of the most worrisome types of industrial waste, not only because of its abundant annual global production but also because it is a notable source of environmental pollution. however, cheese whey can serve as a raw material for the production of biocomposites. in this context, in this study, we assayed the production of a bacteriocin-like inhibitory substance (blis) and lactate by culturing lactobacillus plantarum st16pa in hydrolyzed ...201728784542
comparative pharmacodynamics of single-dose oritavancin and daily high-dose daptomycin regimens against vancomycin-resistant enterococcus faecium isolates in an in vitro pk/pd model of infection.there are limited therapeutic options to treat infections caused by vancomycin-resistant enterococcus faecium (vrefm). the lipoglycopeptide oritavancin exhibits in vitro activity against this pathogen although its utility against infections caused by vrefm has not been established clinically. in this study, the pharmacodynamic activity of free-drug levels associated with 12 mg/kg/day of daptomycin and a single 1200 mg dose of oritavancin were determined against three vana vrefm isolates in an in ...201728784674
chemical sterilization of allograft dermal tissues.common terminal sterilization methods are known to alter the natural structure and properties of soft tissues. one approach to providing safe grafts with preserved biological properties is the combination of a validated chemical sterilization process followed by an aseptic packaging process. this combination of processes is an accepted method for production of sterile healthcare products as described in ansi/aami st67:2011. this article describes the validation of the peracetic acid and ethanol- ...201728799106
genomic insights into the pathogenicity and environmental adaptability of enterococcus hirae r17 isolated from pork offered for retail sale.genetic information about enterococcus hirae is limited, a feature that has compromised our understanding of these clinically challenging bacteria. in this study, comparative analysis was performed of e. hirae r17, a daptomycin-resistant strain isolated from pork purchased from a retail market in beijing, china, and three other enterococcal genomes (enterococcus faecium do, enterococcus faecalis v583, and e. hirae atcc(™) 9790). some 1,412 genes were identified that represented the core genome t ...201728799224
vancomycin-resistant enterococcus faecium st17 outbreak in a neonatal unit. 201728807390
[distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015: results from a multicenter, retrospective study].objective: to describe the distribution and drug resistance of pathogens at hematology department of jiangsu province from 2014 to 2015 to provide reference for empirical anti-infection treatment. methods: pathogens were from hematology department of 26 tertiary hospitals in jiangsu province from 2014 to 2015. antimicrobial susceptibility testing was carried out according to a unified protocol using kirby-bauer method or agar dilution method. collection of drug susceptibility results and corresp ...201728810329
complete genome sequence of a novel, virulent ahjdlikevirus bacteriophage that infects enterococcus faecium.a novel virulent bacteriophage named vb_efap_ime199 that specifically infects enterococcus faecium was isolated and characterized. its optimal multiplicity of infection was 0.01, and it had a 30 minute outbreak period. high-throughput sequencing revealed that the phage has a dsdna genome of 18,838 bp with 22 open reading frames. the genome has very low homology to all other bacteriophage sequences in the genbank database. run-off sequencing experiments confirmed that vb_efap_ime199 has short inv ...201728812171
pathobactyper: a web server for pathogenic bacteria identification and molecular genotyping.with the decline in the cost of whole-genome sequencing because of the introduction of next-generation sequencing (ngs) techniques, many public health and clinical laboratories have started to use bacterial whole genomes for epidemiological surveillance and clinical investigation. for epidemiological and clinical purposes in this "ngs era," whole-genome-scale single nucleotide polymorphism (wgsnp) analysis for genotyping is considered suitable. in this paper, we present an online service, pathob ...201728824598
effectiveness of automated ultraviolet-c light for decontamination of textiles inoculated with enterococcus faecium.healthcare textiles are increasingly recognized as potential vehicles for transmission of hospital-acquired infections. we tested the ability of an automated ultraviolet-c (uv-c) room disinfection device (tru-d(®) smart uvc) to decontaminate textiles inoculated with enterococcus faecium in a clinical setting. contaminated polycotton (50/50 polyester/cotton) swatches were distributed to predefined locations in a ward room and exposed to uvc light. uvc-decontamination reduced e. faecium counts by ...201728827192
reversible inactivation of a peptidoglycan transpeptidase by a β-lactam antibiotic mediated by β-lactam-ring recyclization in the enzyme active site.β-lactam antibiotics act as suicide substrates of transpeptidases responsible for the last cross-linking step of peptidoglycan synthesis in the bacterial cell wall. nucleophilic attack of the β-lactam carbonyl by the catalytic residue (ser or cys) of transpeptidases results in the opening of the β-lactam ring and in the formation of a stable acyl-enzyme. the acylation reaction is considered as irreversible due to the strain of the β-lactam ring. in contradiction with this widely accepted but poo ...201728831100
characterization of microbiota in arapaima gigas intestine and isolation of potential probiotic bacteria.the aim of this study was to determine the intestinal microbiota of pirarucu (a. gigas) in different growth stages (adult and fingerlings), and to isolate and identify potential probiotic bacteria.201728833934
comparison of a new matrix-assisted laser desorption/ionization time-of-flight mass spectrometry platform, asta microidsys, with bruker biotyper for species identification.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, with its accuracy and speed, is widely used for bacterial identification. the asta microidsys system (asta, korea) was recently developed for species identification. we compared its performance with that of bruker biotyper (bruker daltonics, germany). microbes were recovered from sputum, urine, and pus samples from patients admitted to a tertiary care hospital in korea from january to april 2016. matrix solution (α-cya ...201728840993
meso-dihydroguaiaretic acid derivatives with antibacterial and antimycobacterial activity.thirty-three meso-dihydroguaiaretic acid (meso-dga) derivatives bearing esters, ethers, and amino-ethers were synthesized. all derivatives were tested against twelve drug-resistant clinical isolates of gram-positive and gram-negative bacteria, including sensitive (h37rv) and multidrug-resistant mycobacterium tuberculosis strains. among the tested compounds, four esters (7, 11, 13, and 17), one ether (23), and three amino-ethers (30, 31, and 33) exhibited moderate activity against methicillin-res ...201728844400
in vitro antimicrobial activity screening of xylaria hypoxylon.fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey.201628852719
diversity of antagonistic bacteria isolated from medicinal plant peganum harmala l.the antimicrobial activity of plant extract of peganum harmala, a medicinal plant has been studied already. however, knowledge about bacterial diversity associated with different parts of host plant antagonistic to different human pathogenic bacteria is limited. in this study, bacteria were isolated from root, leaf and fruit of plant. among 188 bacterial isolates isolated from different parts of the plant only 24 were found to be active against different pathogenic bacteria i.e. escherichia coli ...201728855824
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