Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the effects of an advanced uterine environment on embryonic survival in the mare. | reasons for performing the study: during embryo transfer (et) the equine embryo can tolerate a wide degree of negative asynchrony but positive asynchrony of >2 days usually results in embryonic death. there is still confusion over whether this is due to the inability of the embryo to induce luteostasis or to an inappropriate uterine environment. objectives: to assess embryo survival and development in an advanced uterine environment. hypothesis: embryo-uterine asynchrony, not the embryo's inabil ... | 2011 | 21950418 |
| a pilot comparison of laser-assisted vs piezo drill icsi for the in vitro production of horse embryos. | intracytoplasmic sperm injection (icsi) is the method of choice for the in vitro production (ivp) of equine embryos. however, conventional icsi has been associated with mechanical damage to the oocyte caused by the deformation of the zona pellucida (zp) and exposure of the oolemma to negative pressure during injection. introduction of the less traumatic and more efficient piezo drill-assisted icsi (pdai) yielded higher cleavage rates and more consistent results. nevertheless, pdai is also associ ... | 2011 | 21950451 |
| characteristics of thoroughbred and quarter horse racehorses that sustained a complete scapular fracture. | reasons for performing study: to determine if scapular fractures occur in racehorses with distinctive characteristics. objectives: to test the hypothesis that thoroughbred (tb) and quarter horse (qh) racehorses with a scapular fracture have similar characteristics that are different from those of their respective racetrack populations. methods: necropsy findings, case details, last race information and career earnings for tb and qh racehorses that had a scapular fracture in california between 19 ... | 2011 | 21950466 |
| Distribution of Mast Cells in Vaginal, Cervical and Uterine Tissue of Non-pregnant Mares: Investigations on Correlations with Ovarian Steroids. | Mast cells constitute part of the cellular immune system of the genital tract. They play a potential role in cervical remodelling during parturition and are subject to the influence of ovarian steroids. In this study, the influence of oestradiol-17ß and progesterone on the distribution of mast cells in equine vaginal, cervical and uterine tissue was evaluated. Genital tracts were retrieved from healthy mares at a local slaughterhouse. The cervix was divided along the longitudinal axis into five ... | 2011 | 21950580 |
| Immunolocalization of aquaporin-5 in normal human skin and hypohidrotic skin diseases. | Aquaporin (AQP)-5 has been shown to be expressed in the secretory parts of mouse, rat and horse sweat glands. However, the precise localization of AQP-5 in normal and diseased human skin has not been fully determined. The aim of the present study was to further clarify the immunolocalization of AQP-5 in normal human skin and hypohidrotic skin diseases. Normal human scalp skin and biopsies from skin affected by hypohidrotic diseases were analyzed for AQP-5 and/or dermcidin expression by immunohis ... | 2011 | 21950762 |
| Relationships between in vivo microdamage and the remarkable regional material and strain heterogeneity of cortical bone of adult deer, elk, sheep and horse calcanei. | Natural loading of the calcanei of deer, elk, sheep and horses produces marked regional differences in prevalent/predominant strain modes: compression in the dorsal cortex, shear in medial-lateral cortices, and tension/shear in the plantar cortex. This consistent non-uniform strain distribution is useful for investigating mechanisms that mediate the development of the remarkable regional material variations of these bones (e.g. collagen orientation, mineralization, remodeling rates and secondary ... | 2011 | 21951210 |
| 1-(5-bromo-2-hydroxy-4-methoxyphenyl)ethanone [SE1] suppresses pro-inflammatory responses by blocking NF-?B and MAPK signaling pathways in activated microglia. | Unregulated activation of microglia is a key risk factor contributes to neurodegenerative diseases and suppression of this phenomenon is considered as a potential therapeutic target. The compound isolated from sea horse Hippocampus kuda Bleeler; 1-(5-bromo-2-hydroxy-4-methoxyphenyl)ethanone [SE1] was characterized for its ability in suppressing LPS mediated activation of murine BV-2 cells. Despite the presence of various active molecular groups in the structure, SE1 has not well explored for its ... | 2011 | 21951967 |
| evaluation of normalization methods on gelc-ms/ms label-free spectral counting data to correct for variation during proteomic workflows. | normalization of spectral counts (spcs) in label-free shotgun proteomic approaches is important to achieve reliable relative quantification. three different spc normalization methods, total spectral count (tspc) normalization, normalized spectral abundance factor (nsaf) normalization, and normalization to selected proteins (nsp) were evaluated based on their ability to correct for day-to-day variation between gel-based sample preparation and chromatographic performance. three spectral counting d ... | 2011 | 21952779 |
| protein conformation and supercharging with dmso from aqueous solution. | the efficacy of dimethyl sulfoxide (dmso) as a supercharging reagent for protein ions formed by electrospray ionization from aqueous solution and the mechanism for supercharging were investigated. addition of small amounts of dmso to aqueous solutions containing hen egg white lysozyme or equine myoglobin results in a lowering of charge, whereas a significant increase in charge occurs at higher concentrations. results from both near-uv circular dichroism spectroscopy and solution-phase hydrogen/d ... | 2011 | 21953100 |
| Mouse versus rat: Profound differences in meiotic regulation at the level of the isolated oocyte. | Cumulus cell-enclosed oocytes (CEO), denuded oocytes (DO), or dissected follicles were obtained 44-48?hr after priming immature mice (20-23 days old) with 5?IU or immature rats (25-27 days old) with 12.5?IU of equine chorionic gonadotropin, and exposed to a variety of culture conditions. Mouse oocytes were more effectively maintained in meiotic arrest by hypoxanthine, dbcAMP, IBMX, milrinone, and 8-Br-cGMP. Atrial natriuretic peptide, a guanylate cyclase activator, suppressed maturation in CEO f ... | 2011 | 21953615 |
| are nhs foundation trusts able and willing to exercise autonomy? 'you can take a horse to water...'. | foundation trusts (fts) have been a central part of the government's national health service (nhs) reforms in england since 2004. they illustrate the government's claim to decentralization, by granting greater autonomy to high performing organizations. the number of fts has grown steadily, reaching 131 in september 2010, over 50% of eligible trusts. despite this growth, and notwithstanding the fact that organizations which initially became fts were previously high performing, doubts remain about ... | 2011 | 21954235 |
| [Comparative analysis of the informativeness of ISSR markers for estimating genetic diversity of horse breeds]. | The informativeness of six different types of ISSR markers was compared in order to evaluate genetic diversity of Mongolian, Buryat and Tuvinian horse breeds (Equus caballus). The dinucleotide-based ISSR primers ((GA)9C, (AG)9C, and (CA)9G) proved to be of little use to examine genetic polymorphism among horses due to the low number of amplified and polymorphic fragments. The polymorphism information content (PIC) for the dinucleotide-based ISSR primers was 2.88 times lower than that for trinucl ... | 2011 | 21954623 |
| whispering dysphonia in an australian family (dyt4): a clinical and genetic reappraisal. | the designation, dyt4, was assigned to an australian family with whispering dysphonia. the role of known causes of dystonia has not been comprehensively investigated in this family, nor has the possible relationship with wilson disease (wnd) in 2 siblings. eighteen family members were neurologically examined, and dna samples were obtained. linkage analysis was performed to dyt1, dyt6, dyt7, dyt11, dyt13, dyt15, and atp7b with microsatellite markers and the thap1 (dyt6), prkra (dyt16), and atp7b ... | 2011 | 21956287 |
| Equine ELISPOT assay to study secretion of IFN? and IL-4 from peripheral blood mononuclear cells. | Human and mouse immune system cells are the most frequently used specimens in ELISPOT assays. In an effect to expand the application of ELISPOT assay to other species, we developed matched antibody pairs for ready-to-use kits designed for studying the frequency of equine IFN?- and IL-4-secreting peripheral blood mononuclear cells (PBMCs). Equine PBMCs were stimulated with either concanavalin A (Con A) or calcium ionomycin mixed with phorbol 12-myristate 13-acetate (CaI + PMA). We found that Con ... | 2012 | 21956499 |
| the cellular protein lyric interacts with hiv-1 gag. | human immunodeficiency virus type 1 (hiv-1) gag is the main structural protein driving assembly and release of virions from infected cells. gag alone is capable of self-assembly in vitro, but host factors have been shown to play a role in efficient viral replication and particle morphogenesis within the living cell. in a series of affinity purification experiments, we identified the cellular protein lyric to be an hiv-1 gag-interacting protein. lyric was previously described to be an hiv-inducib ... | 2011 | 21957284 |
| time-dependent changes of cytokines mrna in bronchoalveolar lavage fluid from symptomatic recurrent airway obstruction-affected horses. | during an 18 day test, we measured the cytokine mrna expression (interleukin-1beta [il-1beta], interleukin-8 [il-8], interferon-gamma [ifn-gamma], tumor necrosis factor-alpha [tnf-alpha]) of cells from bronchoalveolar lavage fluid [balf] in five horses previously diagnosed with rao, before and during challenge exposure, and after the desensitization phase which involved dexamethasone treatment and environmental modification. simultaneously, the same cytokine mrna expression of cells from balf in ... | 2011 | 21957726 |
| Effects of nutritional cues on the duration of the winter anovulatory phase and on associated hormone levels in adult female Welsh pony horses (Equus caballus). | Mares have an annual reproductive rhythm, with a phase of inactivity in midwinter. The aim of this study was to determine the impact of food restriction on physiological and metabolic hallmarks of this rhythm. | 2011 | 21958120 |
| ovarian responses and embryo survival in recipient lactating holstein cows treated with equine chorionic gonadotropin. | the objectives of experiment 1 were to determine a dose of ecg that would increase total luteal volume and plasma progesterone (p4) concentration on estrous cycle day 7 in cows. the objectives of experiment 2 were to determine the effects of treating embryo recipient lactating holstein cows with ecg on pregnancy per embryo transfer (p/et). in experiment 1, lactating dairy cows at 63 ± 3 d postpartum (dim) received no treatment (control, n = 10), or 600 (ecg6, n = 19), or 800 (ecg8, n = 19) iu of ... | 2011 | 21958626 |
| the risk of introduction of equine infectious anemia virus into usa via cloned horse embryos imported from canada. | deriving horse oocytes in the usa is hampered by the lack of abattoirs processing horse carcasses which could provide abundant quantities of ovaries from slaughtered mares. therefore, several cloning industries in the usa are attempting to import cloned horse embryos from canada. like any agricultural commodity, cloned embryos pose a risk of introduction of exotic animal diseases into the importing country. under such circumstances, risk assessment could provide an objective, transparent, and in ... | 2011 | 21958631 |
| ultrasonographic features of the mule embryo, fetus and fetal-placental unit. | the aim of this study was to establish baseline ultrasound data concerning the mule conceptus during gestation. ten multiparous trotter mares were artificially inseminated with chilled semen from an amiatino jack donkey. daily transrectal ultrasonography was carried out from the day of ovulation until day 50 of gestation to determine the following: first detection of the embryonic vesicle (ev), mobility phase, ev diameter, day of ev fixation, changes in ev shape, date of yolk sac regression and ... | 2011 | 21958639 |
| Introduction of new atrial fibrillation ablation technology into clinical practice: The cart before the horse? | 2011 | 21959014 | |
| In vitro characterization of EHV-4 gG-deleted mutant. | Equine herpesvirus 4 (EHV-4) is an important pathogen that causes respiratory tract disease in horse populations worldwide. Glycoprotein G (gG) homologs have been identified in several alphaherpesviruses as minor non-essential membrane-anchored glycoproteins. In this study, EHV-4 gG deletion mutant has been generated by using bacterial artificial chromosome technology to investigate the role of gG in viral pathogenesis. Our findings reported here revealed no significant difference between parent ... | 2011 | 21960433 |
| factors associated to theileria equi in equids of two microregions from rio de janeiro, brazil. | serum samples from 714 equids of itaguaí and serrana microregions, rio de janeiro, southeastern brazil, were examined by indirect fluorescent antibody test (titer 1:80) for theileria equi. the prevalence in the microregions and factors associated with seropositivity were evaluated and the prevalence ratio (pr) calculated. the overall prevalence of t. equi infection was 81.09% (n = 579), with higher prevalence (p < 0.05) in the itaguaí (85.43%) when compared to serrana microregion (76.92%). the g ... | 2011 | 21961755 |
| Modeling competitive cytokine adsorption dynamics within hemoadsorption beads used to treat sepsis. | Extracorporeal blood purification is a promising therapeutic modality for sepsis, a potentially fatal, dysfunctional immunologic state caused by infection. Removal of inflammatory mediators such as cytokines from the blood may help attenuate hyper-inflammatory signaling during sepsis and improve patient outcomes. We are developing a hemoadsorption device to remove cytokines from the circulating blood using biocompatible, porous sorbent beads. In this work, we investigated whether competitive ads ... | 2011 | 21962329 |
| 'Care, and not fine stables, make a good horse': Addressing the sustainability and welfare of geriatric horses. | 2011 | 21962827 | |
| oxidant-antioxidant imbalance in horses infected with equine infectious anaemia virus. | this study assesses the impact of equine infectious anaemia virus (eiav) infection on the oxidant/antioxidant equilibrium of horses. blood samples from 96 romanian horses aged 1-25years, were divided into different groups according to their eiav-infection status, age, and time post-seroconversion. the effect of infection on oxidative stress was estimated by measuring enzymatic antioxidants (superoxide dismutase [sod], glutathione peroxidase [gpx] and catalase), non-enzymatic antioxidants (uric a ... | 2011 | 21962828 |
| network simulation modeling of equine infectious anemia in the non-racehorse population in japan. | an equine infectious anemia (eia) transmission model was developed by constructing a network structure of horse movement patterns in a non-racehorse population. this model was then used to evaluate the effectiveness and efficiency of several eia surveillance strategies. because eia had not been detected in japan since 1993, it was appropriate to review the current surveillance strategy, which aims to eradicate eia by intensive testing, and to consider alternative strategies suitable for the curr ... | 2012 | 21963256 |
| proteomic and biological characterization of the venom of the redtail coral snake, micrurus mipartitus (elapidae), from colombia and costa rica. | venoms of the redtail coral snake micrurus mipartitus from colombia and costa rica were analyzed by "venomics", a proteomic strategy to determine their composition. proteins were separated by rp-hplc, followed by sds-page, in-gel tryptic digestion, identification by maldi or esi tandem mass spectrometry, and assignment to known protein families by similarity. these analyses were complemented with a characterization of venom activities in vitro and in vivo. proteins belonging to seven families we ... | 2011 | 21963438 |
| genetic characterization of endangered kiso horse using 31 microsatellite dnas. | in order to contribute to conservation of the endangered kiso horse, we clarified their genetic information using 31 microstellite dnas, and genotyped 125 horses, 83% of the existing breed. first, we clarified the current status of the horse. the horses were confirmed to have experienced rapid loss of population causing a bottleneck, and their effective population size was much smaller than their census size. moreover, the number of alleles (6.3), observed heterozygosity (0.674), and expected he ... | 2011 | 21963881 |
| Horse metabolism and the photocatalytic process as a tool to identify metabolic products formed from dopant substances: the case of sildenafil. | Two horses were treated with sildenafil, and its metabolic products were sought in both urine and plasma samples. Prior to this, a simulative laboratory study had been done using a photocatalytic process, to identify all possible main and secondary transformation products, in a clean matrix; these were then sought in the biological samples. The transformation of sildenafil and the formation of intermediate products were evaluated adopting titanium dioxide as photocatalyst. Several products were ... | 2011 | 21964727 |
| Liquid chromatography electrospray ionization tandem mass spectrometry for the detection of mesocarb abuse in horse doping. | A method is described for the determination of mesocarb abuse in equestrian sport by combining gradient liquid chromatography and electrospray ionization tandem mass spectrometry. Mesocarb was administrated orally to two horses at a dose of 50 µg/kg. Urine samples were collected up to 120?h post administration. Hydrolyzed and conjugated urine fractions were handled using liquid-liquid extraction (LLE). The identity of the parent drug and metabolites was confirmed using liquid chromatography comb ... | 2011 | 21964865 |
| A trojan horse strategy to deliver amikacin to mycobacterial granulomas. | 2011 | 21965019 | |
| [ocular changes after hormone replacement therapy in postmenopausal women. a preliminary report]. | ocular function is modified with hormone therapy; however, reports in literature are highly controversial. | 2005 | 21966755 |
| Effects of flushing and hormonal treatment on reproductive performance of Iranian Markhoz goats. | Forty-eight Iranian Markhoz goats were allocated to six groups (n = 8) to study the effect of flushing and hormonal treatments on reproductive performance. Treatments were divided into two categories including, hormonal treatments and flushing. The goats in each group were fed the same basal ration and received one of the following treatments: Groups A and B - injection of GnRH and equine chorionic gonadotropin (eCG) respectively; Groups C, D and E - a supplement of barley grain, soybean oil and ... | 2011 | 21967729 |
| Heminecrolysin, a potential immunogen for monospecific antivenom production against Hemiscorpius lepturus scorpion. | Serotherapy against Hemiscorpius (H.) lepturus scorpion sting is based on the administration of equine polyvalent antivenom prepared against a mixture of six venoms. In a previous study, we reported the identification of Heminecrolysin, a 33 kDa H. lepturus venom protein endowed with a sphingomyelinase D, hemolytic and dermonecrotic activities. We aimed herein to investigate the capacity of Heminecrolysin to generate antibodies able to neutralize the major physiopathological properties of H. lep ... | 2011 | 21967811 |
| the comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse. | a chondrocyte progenitor population isolated from the surface zone of articular cartilage presents a promising cell source for cell-based cartilage repair. in this study, equine articular cartilage progenitor cells (acpcs) and equine bone marrow-derived stromal cells (bmscs) were compared as potential cell sources for repair. clonally derived bmscs and acpcs demonstrated expression of the cell fate selector gene, notch-1, and the putative stem cell markers stro-1, cd90 and cd166. chondrogenic in ... | 2011 | 21968294 |
| combined hepatic resection with the inferior vena cava and diaphragm and reconstruction using an equine pericardial patch: report of a case. | we herein report a case of combined hepatic resection with inferior vena cava (ivc) and diaphragm resection, and reconstruction using an equine pericardial patch. a 54-year-old woman showed hepatic cancer recurrence on radiological imaging, with invasion to the caudate lobe of the liver, ivc, diaphragm, and adrenal gland. we resected 10 × 5 cm of the diaphragm. after dissecting the hepatic parenchyma, the caudate lobe was connected only to the ivc. clamping of the ivc was performed between the i ... | 2011 | 21969204 |
| back to black bag and horse-and-buggy medicine. | 2011 | 21969414 | |
| standing low-field magnetic resonance imaging appearance of normal collateral ligaments of the equine distal interphalangeal joint. | high- and low-field magnetic resonance (mr) imaging systems are available for clinical diagnosis of collateral desmopathy of the equine distal interphalangeal joint (dij). knowledge of the normal appearance, size, shape,and signal variation of these ligaments on high- and low-field mr images is essential when assessing desmopathy detected by mr imaging. however, there are no descriptions of the normal features of dij collateral ligaments on images obtained with a standing low-field mr system. lo ... | 2011 | 21970005 |
| truth in basic biomedical science will set future mankind free. | it is self-evident that continued wellbeing and prosperity of our species in time to come depends upon a steady supply of major scientific and technologic innovations. however, major scientific and technical innovations are rare. as a rule, they grow only in the exceptionally fertile minds of men and women, who have fully mastered the underlying basic sciences. to waken their interest in science at an early critical age and to nurture and enhance that interest afterward, good textbooks at all le ... | 2011 | 21970156 |
| dose-response effects of estrogenic mycotoxins (zearalenone, alpha- and beta-zearalenol) on motility, hyperactivation and the acrosome reaction of stallion sperm. | the aim of this study was to investigate the in vitro effects of the fusarium fungus-derived mycotoxin, zearalenone and its derivatives alpha-zearalenol and beta-zearalenol on motility parameters and the acrosome reaction of stallion sperm. since the toxic effects of zearalenone and its derivatives are thought to result from their structural similarity to 17beta-estradiol, 17beta-estradiol was used as a positive control for 'estrogen-like' effects. | 2011 | 21970729 |
| seroprevalence of leptospira spp. in clinically healthy horses in switzerland. | a retrospective, cross-sectional study was conducted to determine the leptospiral seroprevalence in clinically healthy horses in switzerland. a representative sample of 615 horse sera was examined by microscopic agglutination test for the presence of antibodies against 15 leptospira spp. serovars. in total, 58.5 % (n = 360) of the horses were positive for one or more of the antigens analysed, with 20.3 % of them showing titres >= 400. the most prevalent serovar was pyrogenes (22.6 %), followed b ... | 2011 | 21971672 |
| [Intrathoracic esophageal perforation of unknown cause in four horses]. | Three horses (age 17 - 23 years) were referred to the equine clinic of the University of Berne due to colic, fever, tachycardia and tachypnea. All horses showed pleural effusion. Clinical findings in 2 of the horses were highly suggestive of an intra-thoracic esophageal perforation. Severe septic pleuropneumonia without suspicion of an esophageal lesion was diagnosed in the 3rd horse. In addition, an 11 year old stallion was referred to the equine clinic for treatment of a presumptive large colo ... | 2011 | 21971675 |
| Alphaviruses: Semliki forest virus and Sindbis virus vectors for gene transfer into neurons. | Alphaviral vectors based on Semliki Forest virus and Sindbis virus infect many host cell types, causing rapid and high-level transgene expression. In the CNS, Semliki Forest virus and Sindbis virus exhibit an outstanding preference for neurons rather than glial cells, compared to other viruses. Generation of high-titer virus stocks is rapid (less than two days) and typically requires biosafety level 1 or 2 containment. Wild-type vectors are cytotoxic, permitting short-term transgene expression. ... | 2011 | 21971849 |
| Predicting the severity of acute pancreatitis: choose the right horse before hitching the cart. | 2011 | 21971951 | |
| [lumbar canal stenosis with overactive bladder presenting urinary incontinence developed by walking: a case report]. | a 74-year-old male complained of lower back pain, paresthesia of the bilateral feet and urinary incontinence. urinary incontinence manifested at rest and worsened with walking. he had a history of surgery for prostatic cancer. lumbar magnetic resonance imaging (mri) demonstrated lumbar canal stenosis at the l4/5 level. the investigations including a cystometrogram manifested an overactive bladder caused by lumbar canal stenosis. his clinical symptoms were unresponsive to conservative treatment a ... | 2011 | 21972188 |
| pathogen associated molecular pattern motifs from gram-positive and gram-negative bacteria induce different inflammatory mediator profiles in equine blood. | although the incidence of gram-positive infection in horses is increasing, little is known about differences in inflammatory response between gram-positive and gram-negative organisms in this species. equine blood was stimulated with components of both gram-negative and gram-positive organisms: lipopolysaccharide (lps); lipoteichoic acid (lta); peptidoglycan (pg); with combinations of lps, lta and pg; and with phosphate buffered saline (control). lps, lta and pg stimulated tumor necrosis factor ... | 2011 | 21974971 |
| outbreak of equine herpesvirus myeloencephalopathy in france: a clinical and molecular investigation. | equid herpesvirus 1 (ehv-1)-associated myeloencephalopathy (ehm) is a disease affecting the central nervous system of horses. despite the constantly increasing interest about this syndrome, epidemiological data are limited especially when related to the description of large outbreaks. the aim of this article is to describe clinical, virological and molecular data obtained throughout a severe outbreak of ehm, with emphasis on laboratory diagnostic methods. the epidemic disease concerned a riding ... | 2011 | 21975071 |
| effect of ecg on early resumption of ovarian activity in postpartum dairy cows. | the purpose of the present study was to hasten the resumption of ovarian activity early postpartum in lactating dairy cows, using equine chorionic gonadotropin (ecg), to enhance follicular growth, followed by hcg, to induce ovulation. primiparous holstein dairy cows (n=21) were assigned equally into ecg, ecg-hcg and control groups. cows in the ecg and ecg-hcg groups received an i.m. injection of ecg (500 iu folligon®) on day 6 postpartum. cows in the ecg-hcg group were also given an i.m. injecti ... | 2011 | 21975305 |
| Infection prevalences of common tick-borne pathogens in adult lone star ticks (Amblyomma americanum) and American dog ticks (Dermacentor variabilis) in Kentucky. | Rocky Mountain spotted fever, Lyme disease, and ehrlichiosis are tick-borne diseases that are reported annually in Kentucky. We conducted a survey to describe infection prevalence of tick-borne pathogens in Amblyomma americanum and Dermacentor variabilis ticks collected in Kentucky. During 2007-2008, we collected 287 ticks (179 D. variabilis and 108 A. americanum) from canine, feral hog, horse, raccoon, white-tailed deer, and human hosts in six counties in Kentucky. Ticks were screened for Ricke ... | 2011 | 21976578 |
| the cargo-binding domain of transportin 3 is required for lentivirus nuclear import. | lentiviruses, unlike the gammaretroviruses, are able to infect nondividing cells by transiting through nuclear pores to access the host genomic dna. several nuclear import and nuclear pore components have been implicated as playing a role in nuclear import, including transportin 3 (tnpo3), a member of the importin-β family of nuclear import proteins. we demonstrated that tnpo3 was required by several lentiviruses, with simian immunodeficiency virus mac239 (sivmac239) and equine infectious anemia ... | 2011 | 21976643 |
| equine cd4(+) cd25(high) t cells exhibit regulatory activity by close contact and cytokine-dependent mechanisms in vitro. | horses are particularly prone to allergic and autoimmune diseases, but little information about equine regulatory t cells (treg) is currently available. the aim of this study therefore was to investigate the existence of cd4(+) treg cells in horses, determine their suppressive function as well as their mechanism of action. freshly isolated peripheral blood mononuclear cells (pbmc) from healthy horses were examined for cd4, cd25 and forkhead box p3 (foxp3) expression. we show that equine foxp3 is ... | 2011 | 21977999 |
| The molecular epidemiology of equine influenza in Ireland from 2007-2010 and its international significance. | Reasons for performing the study: Antigenic and genetic drift of equine influenza (EI) virus is monitored annually by the Expert Surveillance Panel (ESP), which make recommendations on the need to update vaccines. Surveillance programmes are essential for this process to operate effectively and to decrease the risk of disease spread through the international movement of subclinically infected vaccinated horses. Not only is surveillance necessary to inform vaccine companies which strains are in c ... | 2011 | 21978127 |
| Genomic structure, polymorphism and expression of the horse alpha-actinin-3 gene. | Gene characterization is an important feature for genome annotation and more particularly for candidate genes that could be selected in domestic species. Associations between an alpha-actinin-3 gene polymorphism and muscle performance were reported in humans involving a nonsense mutation (R577X) and in mice after inactivation of the gene. Here, we characterized the equine alpha-actinin-3 (ACTN3) gene by sequencing and transcript analysis. The cDNA was determined to be 3.47 kb in length with an o ... | 2012 | 21979116 |
| breast tenderness after initiation of conjugated equine estrogens and mammographic density change. | we examined the association between new-onset breast tenderness and change in mammographic density after initiation of conjugated equine estrogens (cee). we analyzed baseline, year 1 and 2 data from 695 participants of the women's health initiative estrogen + progestin (daily cee 0.625 mg + medroxyprogesterone acetate 2.5 mg [mpa] or placebo) and estrogen-alone (cee 0.625 mg or placebo) trials who participated in the mammogram density ancillary study. using multivariable repeated measures models ... | 2011 | 21979747 |
| identifying hendra virus diversity in pteropid bats. | hendra virus (hev) causes a zoonotic disease with high mortality that is transmitted to humans from bats of the genus pteropus (flying foxes) via an intermediary equine host. factors promoting spillover from bats to horses are uncertain at this time, but plausibly encompass host and/or agent and/or environmental factors. there is a lack of hev sequence information derived from the natural bat host, as previously sequences have only been obtained from horses or humans following spillover events. ... | 2011 | 21980413 |
| pulling rabbits to reveal the secrets of the prion protein. | the prion protein (prp) is a membrane-tethered glycoprotein that plays a central role in a unique class of neurodegenerative diseases that affect humans and other mammals. prion diseases have genetic and sporadic origins, but their infectious nature sets them apart from other neurodegenerative disorders. according to the "protein-only" hypothesis, misfolded prp conformers (prions) are responsible for both spongiform degeneration of the brain and disease transmissibility. thus, understanding prp ... | 2011 | 21980555 |
| African horse sickness virus induces apoptosis in cultured mammalian cells. | Infection of mammalian cell cultures with African horse sickness virus (AHSV) is known to result in dramatic cytopathic effects (CPE), but no CPE is observed in infected insect cell cultures despite productive virus replication. The basis for this phenomenon has not yet been investigated, but is suggestive of apoptosis being induced following virus infection of the mammalian cells. To investigate whether AHSV can induce apoptosis in infected mammalian cells, Culicoides variipennis (KC) insect ce ... | 2011 | 21983259 |
| A comparison of antibody responses to commercial equine influenza vaccines following primary vaccination of Thoroughbred weanlings--a randomised blind study. | Many racing authorities, sales companies and equestrian bodies have mandatory vaccination policies for equine influenza (EI). The consequences of lack of vaccine efficacy include clinical disease, disruption to training programmes, the cancellation of equestrian events and the introduction of virus to susceptible populations. The correlation between antibody against the virus haemagglutinin and protection against influenza has been well established. The objective of this study was to compare the ... | 2011 | 21983355 |
| vascular access: an historical perspective from sir william harvey to the 1956 nobel prize to andré f. cournand, werner forssmann, and dickinson w. richards. | sir william harvey (1578-1657), who had many precursors, discovered blood circulation in 1628 after a significant number of anatomic dissection of cadavers; his studies were continued by sir christopher wren and daniel johann major. the first central vein catheterization was performed on a horse by stephen hales, an english vicar. in 1844, a century later, the french biologist claude bernard attempted the first carotid artery cannulation and repeated the procedure in the jugular vein, again on a ... | 2011 | 21983826 |
| current situation with equine ai technicians 'unsafe'. | 2011 | 21984625 | |
| round-bale feeder design affects hay waste and economics during horse feeding. | many horse owners find round bales convenient, less labor intensive and more affordable than other hay types, but report an inability to control horse weight gain and excessive hay waste. the objectives were to compare hay waste, hay intake and payback of nine round-bale feeders and a no-feeder control when used during horse feeding. nine round-bale feeders were tested: cinch net, cone, covered cradle, hayhut, hay sleigh, ring, tombstone, tombstone saver and waste less. each feeder design was pl ... | 2011 | 21984721 |
| endoscopic assessment of airway inflammation in horses. | comprehensive endoscopic scoring of the upper and lower airways for inflammation has not been critically assessed among a large population of horses. the relationship between upper and lower airways described in humans by the "one airway, one disease" concept might also apply to horses. | 2011 | 21985142 |
| a comparison of hypertonic (7.2%) and isotonic (0.9%) saline for fluid resuscitation in horses: a randomized, double-blinded, clinical trial. | hypertonic saline solution (7.2%) (hss) can quickly replace intravascular volume deficits. hss more recently has been advocated in the treatment of traumatic brain injury, but its use in dehydrated patients remains controversial. | 2011 | 21985143 |
| computed tomographic findings in the pituitary gland and brain of horses with pituitary pars intermedia dysfunction. | pituitary pars intermedia dysfunction (ppid) is the most common endocrinologic disorder of aged horses. | 2011 | 21985144 |
| laboratory, electrocardiographic, and echocardiographic detection of myocardial damage and dysfunction in an arabian mare with nutritional masseter myodegeneration. | 2011 | 21985146 | |
| t-cell lymphoma with immune-mediated anemia and thrombocytopenia in a horse. | 2011 | 21985147 | |
| Use of an inguinal approach adapted from equine surgery for cryptorchidectomy in dogs and cats: 26 cases (1999-2010). | OBJECTIVE-To determine whether a surgical technique used in cryptorchid horses can be used successfully to remove testicles retained in the inguinal region or abdominal cavity in dogs and cats. DESIGN-Retrospective case series. ANIMALS-22 dogs and 4 cats with cryptorchidism. PROCEDURES-In 1999 through 2010, 26 cryptorchid patients underwent surgery during which an incision was made over the inguinal ring and the undescended testicle was located for removal via identification of the vaginal proce ... | 2011 | 21985352 |
| Penetrating injuries to the frog (cuneus ungulae) and collateral sulci of the foot in equids: 63 cases (1998-2008). | Objective-To determine the outcome of penetrating injuries to the central region of the foot in equids and identify factors that may affect treatment and outcome. Design-Retrospective case series. Animals-63 equids (61 horses, 1 pony, and 1 mule). Procedures-Records of equids incurring puncture wounds through the frog (cuneus ungulae) or collateral sulci of the foot between 1998 and 2008 were reviewed. Evaluated factors that were hypothesized to affect outcome included signalment, degree o ... | 2011 | 21985353 |
| heterobilharzia americana infection as a cause of hepatic parasitic granulomas in a horse. | case description-a 22-year-old american paint horse gelding from the gulf coast region of texas was evaluated for regrowth of a perirectal squamous cell carcinoma that had been surgically removed 11 months previously. clinical findings-a necrotic and ulcerated mass was present below the anus. the horse had paraphimosis and was having difficulty with urination. histologic examination of the mass revealed that it was squamous cell carcinoma, and the horse was euthanized because of the unlikelihood ... | 2011 | 21985355 |
| vasodilatory effect of pentoxifylline in isolated equine digital veins. | the direct vasodilatory action of pentoxifylline (1-(5-oxohexyl)-3,7-dimethylxanthine) and its signalling pathway was evaluated in equine digital veins. cumulative concentration-response curves to pentoxifylline (1nm to 300μm) were recorded in phenylephrine-precontracted equine digital vein rings under different experimental conditions. relaxation to pentoxifylline was partially inhibited by endothelium removal, but was unaltered by cgs-15943 (a non-xanthine adenosine receptor antagonist; 3μm). ... | 2011 | 21986319 |
| influence of different mixing ratios on in-vessel co-composting of sewage sludge with horse stable straw bedding waste: maturity and process evaluation. | composting sewage sludge alone would reduce the decomposition efficiency due to free limited porosity in sludge. to alleviate this, the use of horse stable straw bedding waste (hsb) was evaluated as a co-composting material with sewage sludge in a 10 tonnes day(-1) in-vessel composter for a period of 7 days before curing in a static aeration pile. sludge was mixed with hsb at 1 : 1.5 (hsl) and 1 : 2.9 (lsl) on a fresh weight basis. after a composting period of 56 days, both mixing ratios demonst ... | 2011 | 21987410 |
| Comparison of Gene-Specific DNA Methylation Patterns in Equine Induced Pluripotent Stem Cell Lines with Cells Derived From Equine Adult and Fetal Tissues. | Cellular pluripotency is associated with expression of the homeobox transcription factor genes NANOG, SOX2, and POU5F1 (OCT3/4 protein). Some reports suggest that mesenchymal progenitor cells (MPCs) may express increased quantities of these genes, creating the possibility that MPCs are more "pluripotent" than other adult cell types. The objective of this study was to determine whether equine bone marrow-derived MPCs had gene expression or DNA methylation patterns that differed from either early ... | 2011 | 21988203 |
| Effect of equestrian therapy and onotherapy in physical and psycho-social performances of adults with intellectual disability: a preliminary study of evaluation tools based on the ICF classification. | Purpose: To assess the effects of equestrian rehabilitation (ER) and onotherapy (Ono) on physical and psycho-social performances of subjects affected by intellectual disability (ID), and to develop a measurement tool based on the International Classification of Functioning Disability and Health-Children and Youth (ICF-CY). Methods: A tool based on the ICF-CY classification was designed to evaluate subjects undergoing equine rehabilitation within a bio-psychosocial approach. A simplified version ... | 2012 | 21988646 |
| Effects of a constant rate infusion of detomidine on cardiovascular function, isoflurane requirements and recovery quality in horses. | To examine the influence of a detomidine constant rate infusion (CRI) on cardiovascular function, isoflurane requirements and recovery quality in horses undergoing elective surgery. | 2011 | 21988809 |
| quantitative analysis of a quaternary ammonium drug: ipratropium bromide by lc/esi-ms(n) in horse plasma and urine. | a quantitative method, using lc/esi-ms(n) with a quadrupole linear ion trap mass analyzer, has been developed for the analysis of ipratropium cation in horse plasma and urine. the method applies solid-phase extraction with wcx cartridges for plasma and mm2 cartridges for urine, prior to analysis by lc/esi-ms(n) . the efficiency of extraction combined with the sensitivity and the selectivity of ms(n) allows for the quantification of ipratropium cation at picogram per milliliter levels. the anal ... | 2011 | 21989922 |
| gene expression analysis in the thalamus and cerebrum of horses experimentally infected with west nile virus. | gene expression associated with west nile virus (wnv) infection was profiled in the central nervous system of horses. pyrosequencing and library annotation was performed on pooled rna from the cns and lymphoid tissues on horses experimentally infected with wnv (vaccinated and naïve) and non-exposed controls. these sequences were used to create a custom microarray enriched for neurological and immunological sequences to quantitate gene expression in the thalamus and cerebrum of three experimental ... | 2011 | 21991302 |
| Optimization of a Staphylococcus aureus adhesion assay for equine corneocytes. | Meticillin-resistant Staphylococcus aureus (MRSA) causes serious skin and soft-tissue infections of humans and animals. Multiple strains of MRSA have been characterized, and one in particular, designated as strain USA 500, causes infections predominantly of horses and the people who work with them. The purpose of this study was to optimize an assay which could subsequently be used to compare the relative avidity of different S. aureus strains for equine corneocytes. Corneocytes were collected fr ... | 2011 | 21992593 |
| The genome diversity and karyotype evolution of mammals. | ABSTRACT: The past decade has witnessed an explosion of genome sequencing and mapping in evolutionary diverse species. While full genome sequencing of mammals is rapidly progressing, the ability to assemble and align orthologous whole chromosome regions from more than a few species is still not possible. The intense focus on building of comparative maps for companion (dog and cat), laboratory (mice and rat) and agricultural (cattle, pig, and horse) animals has traditionally been used as a means ... | 2011 | 21992653 |
| Neonatal encephalopathy in foals. | Neonatal encephalopathy is a common central nervous system disorder of neonatal foals and human infants, resulting in clinical signs such as lethargy, inappropriate behavior, seizures, and other neurologic deficits. Although neonatal encephalopathy is frequently seen in equine practice, a paucity of veterinary clinical and basic science research data is available. Therefore, the pathophysiologic mechanisms of this disorder in equids, such as energy deprivation, excess excitatory amino acids, and ... | 2011 | 21993991 |
| understanding abdominal ultrasonography in horses: which way is up? | the recent introduction of more affordable and portable ultrasound equipment makes it possible to perform transabdominal ultrasonography on equine patients in the field. clipping the hair is not always necessary: intraabdominal structures can be quickly evaluated by soaking hair with isopropyl alcohol and using a 3.0- to 3.5-mhz curvilinear transducer. the ultrasonographer must be acquainted with the location and architecture of normal intraabdominal structures and simultaneously mindful of the ... | 2011 | 21993992 |
| isolation and differentiation potential of an equine amnion-derived stromal cell line. | stem cells represent an important tool in veterinary therapeutic field such as tissue engineering. in the present study, equine amnion-derived mesenchymal stromal cells were investigated for applications in veterinary science as an alternative source to bone marrow mesenchymal stem cells and adipose stem cells. amnion stromal cells isolation and characterization protocol is described; the in vitro cell growth rate was calculated by measuring viable cell number over 20 days. the expression of ste ... | 2012 | 21994048 |
| Genome-wide association study among four horse breeds identifies a common haplotype associated with in vitro CD3+ T cell susceptibility/resistance to equine arteritis virus infection. | Previously, we have shown that horses could be divided into susceptible and resistant groups based on an in vitro assay using dual-color flow cytometric analysis of CD3+ T cells infected with equine arteritis virus (EAV). Here, we demonstrate that the differences in in vitro susceptibility of equine CD3+ T lymphocytes to EAV infection have a genetic basis. To investigate the possible hereditary basis for this trait, we conducted a genome-wide association study (GWAS) to compare susceptible and r ... | 2011 | 21994447 |
| alphaviruses in gene therapy. | alphaviruses are enveloped single stranded rna viruses, which as gene therapy vectors provide high-level transient gene expression. semliki forest virus (sfv), sindbis virus (sin) and venezuelan equine encephalitis (vee) virus have been engineered as efficient replication-deficient and -competent expression vectors. alphavirus vectors have frequently been used as vehicles for tumor vaccine generation. moreover, sfv and sin vectors have been applied for intratumoral injections in animals implante ... | 2009 | 21994535 |
| Rev variation during persistent lentivirus infection. | The ability of lentiviruses to continually evolve and escape immune control is the central impediment in developing an effective vaccine for HIV-1 and other lentiviruses. Equine infectious anemia virus (EIAV) is considered a useful model for immune control of lentivirus infection. Virus-specific cytotoxic T lymphocytes (CTL) and broadly neutralizing antibody effectively control EIAV replication during inapparent stages of disease, but after years of low-level replication, the virus is still able ... | 2011 | 21994723 |
| characterization of a full-length endogenous beta-retrovirus, eqerv-beta1, in the genome of the horse (equus caballus). | information on endogenous retroviruses fixed in the horse (equus caballus) genome is scarce. the recent availability of a draft sequence of the horse genome enables the detection of such integrated viruses by similarity search. using translated nucleotide fragments from gamma-, beta-, and delta-retroviral genera for initial searches, a full-length beta-retrovirus genome was retrieved from a horse chromosome 5 contig. the provirus, tentatively named eqerv-beta1 (for the first equine endogenous be ... | 2011 | 21994749 |
| development and validation of a method for cd, pb and as analysis in bovine, equine and poultry liver by inductively coupled plasma mass spectrometry. | a method for the determination of as, cd and pb in bovine, equine and poultry liver by icp-ms was developed and validated. samples were digested in a microwave oven using a 10% hno(3) solution. a set of experiments was made according to a central composite design (ccd) for optimisation of the plasma argon flow, nebuliser argon flow and radiofrequency power applied to the plasma. during the validation, rh and ru were evaluated as internal standards and, after validation, the best was rh for pb an ... | 2011 | 21995839 |
| searching for ivermectin resistance in dutch horses. | a study was conducted to evaluate the occurrence of resistance against, in particular, ivermectin in cyathostomins in the netherlands. seventy horse farms were visited between october 2007 and november 2009. in initial screening, faecal samples were collected 2weeks after deworming with either ivermectin, moxidectin or doramectin. pooled faecal samples from a maximum of 10 horses were examined for worm eggs using a modified mcmaster technique and for worm larvae after faecal larval cultures. in ... | 2011 | 21996004 |
| Equid herpesvirus 5-associated dermatitis in a horse-Resembling herpes-associated erythema multiforme. | An equid herpesvirus 5 (EHV-5) infection was detected in lesioned skin from a nine-year-old Holsteiner stallion in the south of Germany. Macroscopically, the animal displayed a non-pruritic, multifocal, pustular dermatitis around both eyes, nostrils and the muzzle, which had been ongoing for one year. Histopathologically, skin lesions were characterized by orthokeratotic to parakeratotic hyperkeratosis, pustular dermatitis, epidermal hyperplasia, apoptotic keratinocytes, a lympho-plasmahistiocyt ... | 2011 | 21996546 |
| differences between epididymal and ejaculated sperm characteristics in donkey. | spermatozoa acquire their motility and fertilizing ability during their passage through the epididymal canal. in the epididymal caput and corpus spermatozoa undergo several biochemical and metabolic changes while the cauda of the epididymis should be considered as the primarily site for storage of the spermatozoa. in the horse spermatozoa from cauda epididymis were collected and frozen, and the fertility of semen assessed. however, no studies have detailed semen characteristics of spermatozoa co ... | 2011 | 21996580 |
| preliminary list of horse flies (diptera, tabanidae) of serbia. | thirty six species of horse flies (tabanidae) were previously known from serbia (europe). the present faunistic study of horse flies (tabanidae) has resulted in the recording of the 4 new species atylotus fulvus (meigen, 1804); tabanus miki brauer in brauer and bergenstamm, 1880; tabanus unifasciatus loew, 1858; and heptatoma pellucens (fabricius, 1776), in the fauna of serbia. the genus heptatoma meigen, 1803 is cited for the first time in the fauna of serbia. 40 species are currently known fro ... | 2011 | 21998507 |
| integrating databases for research on health and performance in small animals and horses in the nordic countries. | in a world of limited resources, using existing databases in research is a potentially cost-effective way to increase knowledge, given that correct and meaningful results are gained.nordic examples of the use of secondary small animal and equine databases include studies based on data from tumour registries, breeding registries, young horse quality contest results, competition data, insurance databases, clinic data, prescription data and hunting ability tests. in spite of this extensive use of s ... | 2011 | 21999438 |
| worldfengur - the studbook of origin for the icelandic horse. | worldfengur is the database that contains and functions as the studbook of origin of the icelandic horse. only pure-bred icelandic horses, whose ancestry can be traced back to iceland entirely, may be registered into worldfengur. the worldfengur project is a joint effort by the faic (farmers association of iceland) and feif (international federation of icelandic horse associations) to construct an official and central database on horses of icelandic origin located all over the world. it is used ... | 2011 | 21999469 |
| Evaluation of catastrophic musculoskeletal injuries in Thoroughbreds and Quarter Horses at three Midwestern racetracks. | To determine the incidence of and compare the types of catastrophic musculoskeletal injuries (CMIs) sustained in Thoroughbreds and Quarter Horses during racing at 3 Midwestern racetracks from 2000 to 2006. | 2011 | 21999798 |
| Hypoxia regulates the expression of extracellular matrix associated proteins in equine dermal fibroblasts via HIF1. | Exuberant granulation tissue (EGT), a fibrotic healing disorder resembling the human keloid, occurs almost exclusively in limb wounds of horses and may be caused in part by a relative state of hypoxia within the wound. | 2012 | 21999945 |
| equine fetal sex determination using circulating cell-free fetal dna (ccffdna). | in this study, polymerase chain reaction (pcr) reamplification of the first pcr product (2nd-pcr) and a qpcr assay were used to detect the sex determining region y (sry) gene from circulating cell-free fetal dna (ccffdna) in blood plasma of pregnant mares to determine fetal sex. the ccffdna was isolated from plasma of 20 thoroughbred mares (5-13 y old) in the final 3 mo of pregnancy (fetal sex was verified after foaling). for controls, plasma from two non-pregnant mares and two virgin mares were ... | 2011 | 22000028 |
| isolation and phylogenetic grouping of equine encephalosis virus in israel. | during 2008-2009 in israel, equine encephalosis virus (eev) caused febrile outbreaks in horses. phylogenetic analysis of segment 10 of the virus strains showed that they form a new cluster; analysis of segment 2 showed ≈92% sequence identity to eev-3, the reference isolate. thus, the source of this emerging eev remains uncertain. | 2011 | 22000361 |
| equine piroplasmosis associated with amblyomma cajennense ticks, texas, usa. | we report an outbreak of equine piroplasmosis in southern texas, usa, in 2009. infection prevalence reached 100% in some areas (292 infected horses). amblyomma cajennense was the predominant tick and experimentally transmitted theileria equi to an uninfected horse. we suggest that transmission by this tick species played a role in this outbreak. | 2011 | 22000367 |
| Identification and phenotypic characterisation of chondroprogenitor cells for the repair of equine articular cartilage. | 2011 | 22001301 | |
| Equine intensive care unit opens at Liverpool. | 2011 | 22001799 |