Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| probiotic supplements beneficially affect tryptophan-kynurenine metabolism and reduce the incidence of upper respiratory tract infections in trained athletes: a randomized, double-blinded, placebo-controlled trial. | prolonged intense exercise has been associated with transient suppression of immune function and an increased risk of infections. in this context, the catabolism of amino acid tryptophan via kynurenine may play an important role. the present study examined the effect of a probiotic supplement on the incidence of upper respiratory tract infections (urti) and the metabolism of aromatic amino acids after exhaustive aerobic exercise in trained athletes during three months of winter training. | 2016 | 27886064 |
| monitoring of wheat lactic acid bacteria from the field until the first step of dough fermentation. | the present work was carried out to retrieve the origin of lactic acid bacteria (lab) in sourdough. to this purpose, wheat lab were monitored from ear harvest until the first step of fermentation for sourdough development. the influence of the geographical area and variety on lab species/strain composition was also determined. the ears of four triticum durum varieties (duilio, iride, saragolla and simeto) were collected from several fields located within the palermo province (sicily, italy) and ... | 2017 | 27889157 |
| maldi-tof ms meets wgs in a vre outbreak investigation. | the use of maldi-tof ms (matrix-assisted laser desorption/ ionization-time of flight mass spectrometry) and wgs (whole genome sequencing) has been described for identification and strain relatedness determination. we describe the complementary use of maldi-tof ms and wgs in a vre (vancomycin-resistant enterococci) outbreak investigation, and discuss some of the challenges with defining strain similarity across these two platforms. although both assays indicated multiple clusters involved in the ... | 2017 | 27889877 |
| enterococcus faecium hdrsef1 protects the intestinal epithelium and attenuates etec-induced il-8 secretion in enterocytes. | the probiotic enterococcus faecium hdrsef1 (ef1) has been shown to have positive effects on piglet diarrhoea, but the mechanism has not yet been elucidated. in this study, using the ipec-j2 cell line to mimic intestinal epithelial cells and enterotoxigenic escherichia coli (etec) k88ac as a representative intestinal pathogen, the mechanism underlying ef1 protection against an enteropathogen was investigated. the results demonstrated that ef1 was effective in displacing k88ac from the ipec-j2 cel ... | 2016 | 27890970 |
| experience with splenic abscess from southern india. | splenic abscess is a rare entity with potentially life threatening complications. sparse recent published data are available documenting the aetiological profile and management of patients with splenic abscess from india. | 2016 | 27891372 |
| detection of a cfr(b) variant in german enterococcus faecium clinical isolates and the impact on linezolid resistance in enterococcus spp. | the national reference centre for staphylococci and enterococci in germany has received an increasing number of clinical linezolid-resistant e. faecium isolates in recent years. five isolates harbored a cfr(b) variant gene locus the product of which is capable of conferring linezolid resistance. the cfr(b)-like methyltransferase gene was also detected in clostridium difficile. antimicrobial susceptibility was determined for cfr(b)-positive and linezolid-resistant e. faecium isolates and two isog ... | 2016 | 27893790 |
| bactericidal and antioxidant properties of essential oils from the fruits dennettia tripetala g. baker. | the antibacterial and antioxidant properties of the essential oils (eos) of unripe and ripe fruits of dennettia tripetala and their potential for the management of infectious and oxidative-stress diseases were investigated in-vitro in this study. | 2016 | 27894288 |
| glycopeptide resistance: links with inorganic phosphate metabolism and cell envelope stress. | antimicrobial resistance is a critical health issue today. many pathogens have become resistant to many or all available antibiotics and limited new antibiotics are in the pipeline. glycopeptides are used as a 'last resort' antibiotic treatment for many bacterial infections, but worryingly, glycopeptide resistance has spread to very important pathogens such as enterococcus faecium and staphylococcus aureus. bacteria confront multiple stresses in their natural environments, including nutritional ... | 2016 | 27894856 |
| nosocomial infections in the icu: pens and spectacles as fomites. | nosocomial infections are a major cause of morbidity and mortality. non-medical objects known as fomites may have a role in their genesis. we investigated the significance of writing pens and spectacles as fomites. the study was conducted at aga khan university hospital, karachi, from july 2013 to september 2013. cultures were taken from pens and/or spectacles of resident nurses, doctors and nursing assistants in intensive care unit (icu). organisms important in icu nosocomial infections were ta ... | 2016 | 27895354 |
| two cases of proteinase 3-anti-neutrophil cytoplasmic antibody (pr3-anca)-related nephritis in infectious endocarditis. | we herein report two cases of proteinase 3-anti-neutrophil cytoplasmic antibody (pr3-anca)-related nephritis in infectious endocarditis. in both cases, the patients were middle-aged men with proteinuria and hematuria, hypoalbuminemia, decreased kidney function, anemia, elevated c-reactive protein (crp) levels, and pr3-anca positivity. each had bacteremia, due to enterococcus faecium in one and streptococcus bovis in the other. one patient received aortic valve replacement therapy for aortic regu ... | 2017 | 27904114 |
| [mortality analysis of enterococcus faecium bloodstream infection in central taiwan]. | bloodstream infections (bsis) due to enterococcus faecium (e. faecium), particularly those due to vancomycin-resistant enterococcus (vre), are still a therapeutic challenge. | 2016 | 27905623 |
| phenotypic and genotypic characterization of bacteriocinogenic enterococci against clostridium botulinum. | the present study aimed to characterize enterococcus faecalis (n = -6) and enterococcus faecium (n = 1) isolated from healthy chickens to find a novel perspective probiotic candidate that antagonize clostridium botulinum types a, b, d, and e. the isolated enterococci were characterized based on phenotypic properties, pcr, and matrix-assisted laser desorption/ionization time of flight (maldi-tof). the virulence determinants including hemolytic activity on blood agar, gelatinase activity, sensitiv ... | 2016 | 27914002 |
| [experience of the selection and maintainence for donor lung: 62 cases attached]. | objective: to summarize the experience of selection and maintainence for the lungs from donation. methods: from january 2015 to june 2016, 62 cases had been evaluated by department of thoracic surgery, first affiliated hospital of guangzhou medical university. all the clinic data had been collected to evaluate the donor lungs, including the basic information, physical examination, results of radiology and laboratory, the bronchoscope, etc. all the satisfactory donors had been harvested and trans ... | 2016 | 27916031 |
| refugees and antimicrobial resistance: a systematic review. | there is a large increase in the numbers of refugees and asylum seekers worldwide and a lack of data on the carriage of antimicrobial resistance in refugee/asylum seeking groups. | 2017 | 27919742 |
| identification of n-arylated nh125 analogues as rapid eradicating agents against mrsa persister cells and potent biofilm killers of gram-positive pathogens. | bacterial biofilms housing dormant persister cells are innately tolerant to antibiotics and disinfectants, yet several membrane-active agents are known to eradicate tolerant bacterial cells. nh125, a membrane-active persister killer and starting point for development, led to the identification of two n-arylated analogues (1 and 2) that displayed improved biofilm eradication potencies compared to the parent compound and rapid persister-cell-killing activities in stationary cultures of methicillin ... | 2017 | 27925693 |
| assessment of virulence factors, antibiotic resistance and amino-decarboxylase activity in enterococcus faecium mxvk29 isolated from mexican chorizo. | enterococcus faecium mxvk29 has the ability to produce an antimicrobial compound that belongs to class iia of the klaenhammer classification, and could be used as part of a biopreservation technology through direct inoculation of the strain as a starter or protective culture. however, enterococcus is considered as an opportunistic pathogen, hence, the purpose of this work was to study the food safety determinants of e. faecium mxvk29. the strain was sensitive to all of the antibiotics tested (pe ... | 2017 | 27930817 |
| a canine-specific probiotic product in treating acute or intermittent diarrhea in dogs: a double-blind placebo-controlled efficacy study. | a double-blind placebo-controlled intervention study on 60 dogs recruited from a pool of canine patients visiting a veterinary practice and diagnosed with acute diarrhea was conducted. the dogs received in randomized manner either a sour-milk product containing three canine-derived lactobacillus sp. probiotics in combination of lactobacillus fermentum vet 9a, l. rhamnosus vet 16a, and l. plantarum vet 14a (2×10(9)cfu/ml), or placebo. stool consistency, general well-being, and the numbers of spec ... | 2016 | 27938673 |
| antimicrobial resistance in enterococcus strains isolated from healthy domestic dogs. | enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. vancomycin and ampicillin minimum inhibitory concentrations (mics) and high-level aminoglycoside resistance (hlar) tests were also performed. isolates showed resist ... | 2016 | 27976593 |
| procalcitonin levels in bloodstream infections caused by different sources and species of bacteria. | the aim of this study was to evaluate procalcitonin (pct) diagnostic accuracy in discriminating gram-negative (gn) from gram-positive (gp) bloodstream infections and determining the relationship between pct levels, infection sites, and pathogen types. | 2017 | 27979420 |
| detection of eskape bacterial pathogens at the point of care using isothermal dna-based assays in a portable degas-actuated microfluidic diagnostic assay platform. | an estimated 1.5 billion microbial infections occur globally each year and result in ∼4.6 million deaths. a technology gap associated with commercially available diagnostic tests in remote and underdeveloped regions prevents timely pathogen identification for effective antibiotic chemotherapies for infected patients. the result is a trial-and-error approach that is limited in effectiveness, increases risk for patients while contributing to antimicrobial drug resistance, and reduces the lifetime ... | 2017 | 27986722 |
| antimicrobial resistance and virulence profile of enterococci isolated from poultry and cattle sources in nigeria. | this study investigated the occurrence, antimicrobial resistance and virulence of enterococcus from poultry and cattle farms. three hundred and ninety samples: cloacal/rectal swabs (n = 260) and manure (n = 130] were processed for recovery of enterococcus species. standard bacteriological methods were used to isolate, identify and characterize enterococcus species for antimicrobial susceptibility and expression of virulence traits. detection of antibiotic resistance and virulence genes was carri ... | 2017 | 27987112 |
| culture methods impact recovery of antibiotic-resistant enterococci including enterococcus cecorum from pre- and postharvest chicken. | pathogenic strains of enterococcus cecorum (ec) expressing multidrug resistance have emerged. in national antimicrobial resistance monitoring system (narms) data, ec is rarely recovered from chickens. two narms methodologies (fda and usda) were compared with standard culture (sc) techniques for recovery of ec. narms methods failed to detect ec in 58 caecal samples, 20 chicken breast or six whole broiler samples. ec was recovered from 1 of 38 (2·6%) and 2 of 38 (5·2%) preharvest spinal lesions (u ... | 2017 | 27987523 |
| enterococcus faecium and enterococcus durans isolated from cheese: survival in the presence of medications under simulated gastrointestinal conditions and adhesion properties. | in this study, we evaluated the survival of enterococcus faecium and enterococcus durans, isolated from cheese, in the presence of medications and under simulated in vitro gastrointestinal conditions. the presence of genes encoding virulence factors, the susceptibility to antimicrobial agents, and adhesion properties were also assessed. enterococcus faecium and e. durans both exhibited resistance to most of the tested medications but showed a large sensitivity to analgesics and antihypertensives ... | 2017 | 27988121 |
| vana-positive multi-drug-resistant enterococcus spp. isolated from surfaces of a us hospital laundry facility. | enterococcus spp. are a normal part of the gastrointestinal tract of humans and animals. they are also important pathogens, being responsible for 14% of us nosocomial infections from 2007 to 2010. | 2017 | 27989378 |
| enterococcus species diversity in fecal samples of wild marine species as determined by real-time pcr. | analyses using culture-independent molecular techniques have improved our understanding of microbial composition. the aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative pcr. seven enterococcus species were examined in fecal dna of south american fur seals (arctocephalus australis), subantarctic fur seals (arctocephalus tropicalis), green turtles (chelonia mydas), magellanic penguins (spheniscus magellanicus), snowy-crown ... | 2017 | 27991828 |
| comparison of the antimicrobial activity of direct pulp-capping materials: mineral trioxide aggregate-angelus and biodentine. | to compare the antimicrobial activity of the tricalcium silicate-based biodentine (bd) and mineral trioxide aggregate (mta)-angelus cement with the aid of agar diffusion test. | 2017 | 27994321 |
| a novel combination approach of human polyclonal ivig and antibiotics against multidrug-resistant gram-positive bacteria. | gram-positive bacteria, especially methicillin-resistant staphylococcus aureus (mrsa) and enterococci, have shown a remarkable ability to develop resistance to antimicrobial agents. | 2016 | 27994476 |
| effects of ex vivo infection with etec on jejunal barrier properties and cytokine expression in probiotic-supplemented pigs. | enterotoxigenic escherichia coli (etec) strains are involved in piglet post-weaning diarrhea. prophylactic measures including probiotics have been examined in infection experiments with live piglets. in the present study, we have tested whether the early effects of etec infection can also be evoked and studied in a model in which etec is added to whole mucosal tissues ex vivo, and whether this response can be modulated by prior supplementation of the piglets with probiotics. | 2017 | 27995406 |
| [epidemiology and risk factors of patients with intra-abdominal postsurgical infection treated with tigecycline: a cohort study]. | to study a cohort of patients with intra-abdominal postsurgical infection treated with tigecycline to analyze its effectiveness and mortality related factors. | 2017 | 28010058 |
| polyclonal emergence of vana vancomycin-resistant enterococcus faecium in australia. | to investigate the genetic context associated with the emergence of vana vre in australia. | 2017 | 28031272 |
| antimicrobial resistance of selected invasive bacteria in a tertiary care center: results of a prospective surveillance study. | we aimed to report the distribution and resistance patterns of eight invasive clinically relevant bacteria surveyed in the clinical center of serbia (ccs) in belgrade. | 2016 | 28036312 |
| comparative genome analysis of multiple vancomycin-resistant enterococcus faecium isolated from two fatal cases. | enterococcus faecium is both a commensal of the human intestinal tract and an opportunistic pathogen. the increasing incidence of enterococcal infections is mainly due to the ability of this organism to develop resistance to multiple antibiotics, including vancomycin. the aim of this study was to perform comparative genome analyses on four vancomycin-resistant enterococcus faecium (vrefm) strains isolated from two fatal cases in a tertiary hospital in malaysia. two sequence types, st80 and st203 ... | 2017 | 28039075 |
| novel lnu(g) gene conferring resistance to lincomycin by nucleotidylation, located on tn6260 from enterococcus faecalis e531. | to identify a novel putative lincosamide resistance gene determinant in a swine enterococcus faecalis e531 exhibiting a lincosamide resistance/macrolide susceptibility (l r m s ) phenotype and to determine its location and genetic environment. | 2017 | 28039271 |
| beneficial effects of probiotic cholesterol-lowering strain of enterococcus faecium wefa23 from infants on diet-induced metabolic syndrome in rats. | the aim of this study was to select probiotic enterococcus strains that have the potential to improve metabolic syndrome (ms). ten enterococcus strains isolated from healthy infants were evaluated for their probiotic properties in vitro, and enterococcus faecium wefa23 was selected due to its cholesterol removal ability (1.89 ± 0.07 mg/10(10) cfu), highest glycodeoxycholic acid-hydrolase activity (1.86 ± 0.01 u/mg), and strong adhesion capacity to caco-2 cells (17.90 ± 0.19%). the safety of e. f ... | 2017 | 28041735 |
| rela mutant enterococcus faecium with multiantibiotic tolerance arising in an immunocompromised host. | serious bacterial infections in immunocompromised patients require highly effective antibacterial therapy for cure, and thus, this setting may reveal novel mechanisms by which bacteria circumvent antibiotics in the absence of immune pressure. here, an infant with leukemia developed vancomycin-resistant enterococcus faecium (vre) bacteremia that persisted for 26 days despite appropriate antibiotic therapy. sequencing of 22 consecutive vre isolates identified the emergence of a single missense mut ... | 2017 | 28049149 |
| tigecycline-based therapy for glycopeptide-resistant enterococcus faecium infection in a pediatric intensive care unit. | 2016 | 28051225 | |
| antimicrobial resistant escherichia coli and enterococcus spp. isolated from miranda donkey (equus asinus): an old problem from a new source with a different approach. | the miranda donkey (equus asinus) is an endangered asinine from miranda do douro region, located in north east of portugal. we study the antimicrobial resistance (amr) and virulence genes in escherichia coli and enterococcus spp. isolates from these animals. in march 2014, a total of 66 faecal samples were recovered from independent animals. antibiotic resistance was determined by the disk diffusion method. carriage of genes codifying for antibiotic resistant and virulent factors was analyzed by ... | 2017 | 28056219 |
| genetic relatedness of faecal coliforms and enterococci bacteria isolated from water and sediments of the apies river, gauteng, south africa. | to date, the microbiological quality of river sediments and its impact on water resources are not included in the water quality monitoring assessment. therefore, the aim of this study was to establish genetic relatedness between faecal coliforms and enterococci isolated from the river water and riverbed sediments of apies river to better understand the genetic similarity of microorganisms between the sediment and water phases. indicator bacteria were subjected to a molecular study, which consist ... | 2017 | 28063147 |
| vancomycin-dependent enterococcus faecium can easily be obscured. | 2016 | 28065416 | |
| design, synthesis and antibacterial evaluation of novel pleuromutilin derivatives possessing piperazine linker. | a series of pleuromutilin derivatives bearing piperazine ring have been reported. the in vitro antibacterial activities of the synthetic derivatives against mrsa (atcc 43300), staphylococcus aureus (atcc 29213), enterococcus faecalis (atcc 29212), enterococcus faecium (atcc35667) and escherichia coli (atcc25922) were evaluated by the broth dilution method. most of the synthesized derivatives displayed potent activities. compounds 11c, 12a and 12c were found to be the most active antibacterial de ... | 2017 | 28068600 |
| a novel phosphodiesterase of the gdpp family modulates cyclic di-amp levels in response to cell membrane stress in daptomycin-resistant enterococci. | substitutions in the liafsr membrane stress pathway are frequently associated with the emergence of antimicrobial peptide resistance in both enterococcus faecalis and enterococcus faecium cyclic di-amp (c-di-amp) is an important signal molecule that affects many aspects of bacterial physiology, including stress responses. we have previously identified a mutation in a gene (designated yybt) in e. faecalis that was associated with the development of daptomycin resistance, resulting in a change at ... | 2017 | 28069645 |
| decarboxylase-positive enterococcus faecium strains isolated from rabbit meat and their sensitivity to enterocins. | the objective of the study was to determine sensitivity of enterococcus faecium strains from rabbit meat to enterocins. | 2017 | 28070313 |
| antibacterial activity of limonium brasiliense (baicuru) against multidrug-resistant bacteria using a statistical mixture design. | limonium brasiliense (boiss.) kuntze (plumbaginaceae) is commonly known as "baicuru" or "guaicuru" and preparations of its dried rhizomes have been popularly used in the treatment of premenstrual syndrome and menstrual disorder, and as an antiseptic in genito-urinary infections. this study evaluated the potential antibacterial activity of rhizome extracts against multidrug-resistant bacterial strains using statistical mixture design. | 2017 | 28089736 |
| efficacy of vacuum steam pasteurization for inactivation of salmonella pt 30, escherichia coli o157:h7 and enterococcus faecium on low moisture foods. | low moisture foods such as nuts, spices, and seeds have been implicated in several outbreaks due to salmonella or e. coli o157:h7 contamination. such foods may be consumed raw, and can be used as ingredients in other food products. while numerous thermal inactivation studies have been conducted for salmonella on nuts, studies on other seeds and grains are minimal. product water activity can influence the thermal resistance of pathogens, where thermal resistance increases as water activity decrea ... | 2017 | 28092821 |
| interspecies transfer of vancomycin, erythromycin and tetracycline resistance among enterococcus species recovered from agrarian sources. | enterococci are now well recognised for their ability to transfer antibiotic resistance and for their association with nosocomial infections, but less is known regarding their relevance in the wider environment. enterococcus faecalis and enterococcus faecium were isolated from a range of agrarian associated sources (low-flow water, septic tank, poultry litter, high flow water, slurry/soil) and were assessed for latent ability to transfer antimicrobial resistance. | 2017 | 28100194 |
| model systems for the study of enterococcal colonization and infection. | enterococcus faecalis and enterococcus faecium are common inhabitants of the human gastrointestinal tract, as well as frequent opportunistic pathogens. enterococci cause a range of infections including, most frequently, infections of the urinary tract, catheterized urinary tract, bloodstream, wounds and surgical sites, and heart valves in endocarditis. enterococcal infections are often biofilm-associated, polymicrobial in nature, and resistant to antibiotics of last resort. understanding enteroc ... | 2017 | 28102784 |
| comparative efficacies of daptomycin, vancomycin, and linezolid in experimental enterococcal peritonitis. | enterococci have become increasingly important pathogens for nosocomial infection (e.g. bacteremia, intra-abdominal infection, endocarditis, etc.), related to their intrinsic resistance to many antibiotics. although the in vitro susceptibility of daptomycin (dap) against enterococci is well established, the food and drug administration has only approved its use for complicated skin and skin structure infections induced by enterococcus faecalis. in this study we evaluated the potential therapeuti ... | 2017 | 28108098 |
| rapid detection of high-risk enterococcus faecium clones by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | we aimed to explore the potential of matrix assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) for early identification of dominant enterococcus faecium (efm) clones involved in human infections. well-characterized efm isolates (n=77), analyzed by pulsed-field gel electrophoresis and multilocus sequence typing(eburst and baps [bayesian analysis of population structure] algorithms), and belonging to different hospital (n=53) and community (n=24) phylogenomic grou ... | 2017 | 28109550 |
| clinical and microbiological characteristics of bloodstream infections among patients with haematological malignancies with and without neutropenia at a medical centre in northern taiwan, 2008-2013. | this study aimed to investigate the epidemiology and microbiology of bloodstream infections (bsis) in patients with haematological malignancies. clinical characteristics and microbiology of 2083 patients with haematological malignancy who were treated for bsi from 2008 to 2013 at a medical centre in taiwan were retrospectively reviewed. lymphoma (38.1%) was the most common, followed by acute myeloid leukaemia (30.9%). of the 2090 non-duplicate bsi isolates, 1310 (62.7%) were recovered from patie ... | 2017 | 28109554 |
| colonisation of poultry by salmonella enteritidis s1400 is reduced by combined administration of lactobacillus salivarius 59 and enterococcus faecium pxn-33. | salmonella enteritidis remains a significant issue within the poultry industry and one potential solution is to use probiotic bacteria to prevent salmonella colonisation through competitive exclusion (ce). we demonstrate that combined administration of lactobacillus salivarius 59 and enterococcus faecium pxn33 were effective competitive excluders of salmonella enteritidis s1400 in poultry. two models were developed to evaluate the efficacy of probiotic where birds received salmonella enteritidis ... | 2017 | 28110775 |
| positive clinical outcomes derived from using a proprietary mixture of selected strains during pregnancy. | while the use of antibiotic prophylaxis is clearly advantageous to prevent streptococcal infection, it alters the composition of the gut microbiota in mothers and infants. enterococcus faecium l3 is one of the best studied probiotic strains and shows strong antagonistic activity against streptococcus agalactiae due to the production of bacteriocins able to inhibit common gut and vaginal pathogens. | 2017 | 28112691 |
| characterization of class iia bacteriocin resistance in enterococcus faecium. | vancomycin-resistant enterococci, particularly resistant enterococcus faecium, pose an escalating threat in nosocomial environments because of their innate resistance to many antibiotics, including vancomycin, a treatment of last resort. many class iia bacteriocins strongly target these enterococci and may offer a potential alternative for the management of this pathogen. however, e. faecium's resistance to these peptides remains relatively uncharacterized. here, we explored the development of r ... | 2017 | 28115354 |
| enterococcus faecium strain r30 increases red blood cell velocity and prevents capillary regression in the soleus of hindlimb unloaded rats via the enos/vegf pathway. | a chronic decrease in neuromuscular activity results in atrophy and capillary regression in skeletal muscles. the purposes of the present study were to determine the effects of enterococcus faecium strain r30 (r30) administration on 1) the hemodynamics of the rat soleus muscle and 2) the capillary regression normally associated with hindlimb unloading (hu). | 2017 | 28116830 |
| effect of preoperative biliary drainage on bacterial flora in bile of patients with periampullary cancer. | patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (pbd). the effect of pbd on the microbiome of the biliary system and on postoperative outcome remains unclear. | 2017 | 28121036 |
| effect of vancomycin, tylosin, and chlortetracycline on vancomycin-resistant enterococcus faecium colonization of broiler chickens during grow-out. | broiler chickens may serve as reservoirs for human colonization by vancomycin-resistant enterococcus (vre). we examined the effects of vancomycin and two commonly used antimicrobial feed additives on vre colonization in broiler chickens during grow-out. chicks received unsupplemented feed or feed containing vancomycin, chlortetracycline, or tylosin from day of hatch to grow-out at 6 weeks. at 3 days of age, chicks received by crop gavage 10(7) colony-forming units (cfus) of a human or poultry vr ... | 2017 | 28128649 |
| [update on antibiotic resistance in gram-positive bacteria]. | antimicrobial resistance among gram-positive bacteria, especially in staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and streptococcus pneumoniae, is a serious threat to public health. these microorganisms have multiple resistance mechanisms to agents currently used in clinical practice. many of these resistance mechanisms are common to all 4 of these bacterial species, but other mechanisms seem to be more specific. the prevalence and dissemination of these mechanisms varies ... | 2017 | 28129816 |
| bioactive compounds from hypericum humifusum and hypericum perfoliatum: inhibition potential of polyphenols with acetylcholinesterase and key enzymes linked to type-2 diabetes. | natural products are reported to have a wide spectrum of pharmacological properties such as antimicrobial, anti-inflammatory and anti-cholinesterase. the genus hypericum (hypericaceae) is a source of a variety of molecules with different biological activities, notably hypericin and various phenolics. | 2017 | 28147885 |
| evolutionary origins of the emergent st796 clone of vancomycin resistant enterococcus faecium. | from early 2012, a novel clone of vancomycin resistant enterococcus faecium (assigned the multi locus sequence type st796) was simultaneously isolated from geographically separate hospitals in south eastern australia and new zealand. here we describe the complete genome sequence of ef_aus0233, a representative st796 e. faecium isolate. we used pacbio single molecule real-time sequencing to establish a high quality, fully assembled genome comprising a circular chromosome of 2,888,087 bp and five ... | 2017 | 28149688 |
| synthesis, surfactant properties and antimicrobial activities of methyl glycopyranoside ethers. | a series of amphiphilic methyl glucopyranoside ethers incorporating various alkyl chain lengths has been synthesized from commercially available methyl glucopyranosides following an acetalisation/hydrogenolysis sequence. the amphiphilic properties of ethers and acetal intermediates were evaluated. both families exhibit excellent surfactant properties with a maximum efficiency obtained for compounds bearing a linear dodecyl chain (cmc = 0.012 mm, γsat. = 30 mn m(-1)). antimicrobial activity studi ... | 2017 | 28157594 |
| a potent synthetic inorganic antibiotic with activity against drug-resistant pathogens. | the acronymously named "eskape" pathogens represent a group of bacteria that continue to pose a serious threat to human health, not only due to their propensity for repeated emergence, but also due to their ability to "eskape" antibiotic treatment. the evolution of multi-drug resistance in these pathogens alone has greatly outpaced the development of new therapeutics, necessitating an alternative strategy for antibiotic development that considers the evolutionary mechanisms driving antibiotic re ... | 2017 | 28165020 |
| prevalence of high-level gentamicin-resistant enterococcus faecalis and enterococcus faecium in an iranian hospital. | this study was designed to determine the molecular characteristics and antimicrobial resistance of enterococcal strains isolated from patients admitted to an iranian hospital. enterococcal strains were isolated from the burn patients. all strains were screened for genes encoding resistance to aminoglycoside [aac(6')-ie-aph(2'')-ia, aph (3'), ant (4')], resistance to vancomycin (vana, vanb), resistance to tetracycline (tetk, tetl, tetm, teto), and resistance to erythromycin (erma, ermb, ermc) by ... | 2016 | 28167856 |
| antibacterial low-molecular-weight compounds produced by the marine bacterium rheinheimera japonica kmm 9513(t). | strain kmm 9513(t) was isolated from a sediment sample collected from the sea of japan seashore and selected due to its ability to inhibit indicator bacterial growth. the strain kmm 9513(t) has been recently described as a novel species rheinheimera japonica. this study was undertaken to determine which substances produced by strain kmm 9513(t) could be responsible for its antimicrobial activity. eight compounds were obtained from an ethyl acetate extract of r. japonica kmm 9513(t). the structur ... | 2017 | 28176144 |
| correlation between infective factors and antibiotic resistance in enterococci clinical isolates in west of iran. | the present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in enterococcus faecalis and enterococcus faecium. considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infections and persistence of enterococci in hospital settings, which can lead to an increase in antimicrobial resistance. 175 e. faecalis and 67 e. faecium isolated from clinical specimens were used. the ... | 2017 | 28184339 |
| current status of the preharvest application of pro- and prebiotics to farm animals to enhance the microbial safety of animal products. | the selection of microorganisms that act as probiotics and feed additives that act as prebiotics is an ongoing research effort, but a sizable range of commercial pro-, pre- and synbiotic (combining pro- and prebiotics) products are already available and being used on farms. a survey of the composition of commercial products available in the united states revealed that lactobacillus acidophilus, enterococcus faecium, and bacillus subtilis were the three most common species in probiotic products. ... | 2017 | 28185614 |
| efficacy of a probiotic-prebiotic supplement on incidence of diarrhea in a dog shelter: a randomized, double-blind, placebo-controlled trial. | diarrhea is the most frequent morbidity affecting kenneled dogs in animal shelters. diarrhea impacts animal welfare and the finances of the shelter as they must treat, clean, and house affected animals until recovered. | 2017 | 28186660 |
| lipoteichoic acid synthesis inhibition in combination with antibiotics abrogates growth of multidrug-resistant enterococcus faecium. | enterococcus faecium is a multidrug-resistant (mdr) nosocomial pathogen causing significant morbidity in debilitated patients. new antimicrobials are needed to treat antibiotic-resistant e. faecium infections in hospitalised patients. e. faecium incorporates lipoteichoic acid (lta) (1,3-polyglycerol-phosphate linked to glycolipid) in its cell wall. the small-molecule inhibitor 1771 [2-oxo-2-(5-phenyl-1,3,4-oxadiazol-2-ylamino)ethyl 2-naphtho[2,1-b]furan-1-ylacetate] specifically blocks the activ ... | 2017 | 28188831 |
| improvement of infection control management by routine molecular evaluation of pathogen clusters. | undetected pathogen clusters can often be a source of spreading in-hospital infections. unfortunately, detection of clusters can be problematic because epidemiological connection is not always easily established. infection prevention and control (ipc) measures, however, are most effective when applied at the earliest possible stage. | 2017 | 28189284 |
| in vitro screening of an fda-approved library against eskape pathogens. | bacterial resistance to conventional antibiotics is an increasingly serious threat to public health worldwide that requires immediate exploration and the development of novel antimicrobial compounds. drug repurposing is an inexpensive and untapped source of new antimicrobial leads, and it holds many attractive features warranting further attention for antimicrobial drug discovery. in an effort to repurpose drugs and explore new leads in the field of antimicrobial drug discovery, we performed a w ... | 2017 | 28190396 |
| analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth. | to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth. | 2017 | 28193569 |
| subinhibitory concentrations of ciprofloxacin enhance antimicrobial resistance and pathogenicity of enterococcus faecium. | enterococcus faecium has emerged as a major opportunistic pathogen for two decades, with the spread of hospital-adapted multidrug-resistant clones. members of the intestinal microbiota, they are subjected to numerous bacterial stresses, including antibiotics at subinhibitory concentrations (sics). since fluoroquinolones are extensively prescribed, sics are very likely to occur in vivo with potential effects on bacterial metabolism with subsequent modulation of opportunistic traits. the aim of th ... | 2017 | 28193670 |
| genotyping of clinical and environmental multidrug resistant enterococcus faecium strains. | multidrug resistant (mdr) enterococcus faecium is a nosocomial pathogen and clonal complex 17 (cc17) is the main genetic subpopulation of e. faecium in hospitals worldwide. | 2017 | 28195096 |
| linezolid-resistant enterococci in polish hospitals: species, clonality and determinants of linezolid resistance. | the significant increase of the linezolid-resistant enterococci (lre) has been observed in polish hospitals since 2012 and our study aimed at elucidating the possible reasons for this phenomenon. polish lre isolates were analysed by multilocus-sequence typing (mlst) and multiple locus variable-number tandem repeat (vntr) analysis (mlva), polymerase chain reaction (pcr) and pcr-restriction fragment length polymorphism (pcr-rflp) to establish clonal relatedness and mechanism of linezolid resistanc ... | 2017 | 28197728 |
| antibacterial effect of sevoflurane and isoflurane. | multidrug resistant bacteria are increasing worldwide and therapeutic options are limited. some anaesthetics have shown antibacterial activity before. in this study, we have investigated the antibacterial effect of the halogenated anaesthetic agents sevoflurane and isoflurane against a range of resistant pathogens. | 2017 | 28198170 |
| diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on listeria monocytogenes and salmonella enterica serovar enteritidis in a co-culture model. | food-borne infections cause huge economic and human life losses. listeria monocytogenes and salmonella enterica serovar enteritidis are among the top ranking pathogens causing such losses. control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to ... | 2017 | 28202007 |
| screening for vancomycin-resistant enterococci with xpert® vana/vanb: diagnostic accuracy and impact on infection control decision making. | vancomycin-resistant enterococci (vre) are increasingly important nosocomial pathogens and screening for colonization status is a mainstay in infection control. we implemented pcr-based screening during vana-positive enterococcus faecium outbreaks in four university hospitals in copenhagen, denmark. xpert(®)vana/vanb was performed directly on rectal swabs and the vana pcr result was used to guide infection control measures. concurrently, all samples were selectively cultured including an overnig ... | 2017 | 28203378 |
| biocompatible injectable hydrogel with potent wound healing and antibacterial properties. | two component injectable hydrogels that cross-link in situ have been used as noninvasive wound-filling devices, i.e., sealants. these materials carry a variety of functions at the wound sites, such as sealing leaks, ceasing unwanted bleeding, binding tissues together, and assisting in wound healing processes. however, commonly used sealants typically lack antibacterial properties. since bacterial infection at the wound site is very common, bioadhesive materials with intrinsic antibacterial prope ... | 2017 | 28207269 |
| multidrug-resistant enterococcal infections: new compounds, novel antimicrobial therapies? | over the past two decades infections due to antibiotic-resistant bacteria have escalated world-wide, affecting patient morbidity, mortality, and health care costs. among these bacteria, enterococcus faecium and enterococcus faecalis represent opportunistic nosocomial pathogens that cause difficult-to-treat infections because of intrinsic and acquired resistance to a plethora of antibiotics. in recent years, a number of novel antimicrobial compound classes have been discovered and developed that ... | 2017 | 28209400 |
| growth interactions and antilisterial effects of the bacteriocinogenic lactococcus lactis subsp. cremoris m104 and enterococcus faecium ke82 strains in thermized milk in the presence or absence of a commercial starter culture. | traditional greek cheeses are often produced from thermized milk (tm) with the use of commercial starter cultures (cscs), which may not inhibit growth of listeria monocytogenes completely. therefore, this study evaluated the behavior of an artificial l. monocytogenes contamination in commercially tm (63 °c; 30 s) inoculated with a csc plus lactococcus lactis subsp. lactis m104 and/or enterococcus faecium ke82, two indigenous strains producing nisin a and enterocin a and b, respectively. inoculat ... | 2017 | 28213019 |
| conjugative type iv secretion in gram-positive pathogens: trag, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein tram. | conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pip501 is a gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in enterococcus faecalis and enterococcus faecium clinical isolates. the pip501 type iv secretion system is encoded by 15 transfer genes. in this work, we focus on the virb1-like protein trag, a modular peptidoglycan metabolizing enzyme, and the virb8-homolog tram, a potenti ... | 2017 | 28219792 |
| comparing thermal process validation methods for salmonella inactivation on almond kernels. | ongoing regulatory changes are increasing the need for reliable process validation methods for pathogen reduction processes involving low-moisture products; however, the reliability of various validation methods has not been evaluated. therefore, the objective was to quantify accuracy and repeatability of four validation methods (two biologically based and two based on time-temperature models) for thermal pasteurization of almonds. almond kernels were inoculated with salmonella enteritidis phage ... | 2017 | 28221874 |
| inactivation of gram-positive bacteria by novel phenolic branched-chain fatty acids. | novel phenolic branched-chain fatty acids (pbc-fas) were evaluated for their antimicrobial properties against both gram-positive ( listeria innocua , bacillus subtilis , enterococcus faecium ) and gram-negative ( escherichia coli , salmonella typhimurium, and pseudomonas tolaasii ) bacteria. in addition, pbc-fa derivatives, such as pbc-fa methyl ester mixture, methyl-branched fatty acid mixtures, and trimethylsilyl-pbc-fa methyl esters, were synthesized to study the structure activity relationsh ... | 2017 | 28221889 |
| enhanced control of listeria monocytogenes by enterococcus faecium ke82, a multiple enterocin-producing strain, in different milk environments. | enterococcus faecium ke82, isolated from traditional greek graviera cheese, was identified in pure broth cultures in vitro as a multiple enterocin-producing bacterial strain possessing the structural enta, entb, and entp enterocin genes. e. faecium ke82 was further assessed for in situ antilisterial activity in raw milk (rm) and commercially thermized milk (tm; 63°c for 30 s) in the presence of the indigenous microbiota and in sterile raw milk (srm; 121°c for 5 min) with or without the addition ... | 2017 | 28221890 |
| isolation, identification, and evaluation of new lactic acid bacteria strains with both cellular antioxidant and bile salt hydrolase activities in vitro. | in this study, we analyzed chinese traditional fermented food to isolate and identify new lactic acid bacteria (lab) strains with novel functional properties and to evaluate their cellular antioxidant and bile salt hydrolase (bsh) activities in vitro. a sequential screening strategy was developed to efficiently isolate and obtain 261 lab strains tolerant of bile salt, acid, and h2o2 from nine chinese traditional fermented foods. among these strains, 70 were identified as having 2,2-diphenyl-1-pi ... | 2016 | 28221912 |
| requirement of the crors two-component system for resistance to cell-wall-targeting antimicrobials in enterococcus faecium. | enterococci are serious opportunistic pathogens that are resistant to many cell-wall-targeting antibiotics. the crors two-component signaling system responds to antibiotic-mediated cell-wall stress and is critical for resistance to cell-wall-targeting antibiotics in enterococcus faecalis here, we identify and characterize an orthologous two-component system found in enterococcus faecium that is functionally equivalent to the crors system of e. faecalis deletion of crors in e. faecium resulted in ... | 2017 | 28223383 |
| raising the alarmone: within-host evolution of antibiotic-tolerant enterococcus faecium. | enterococci are ancient commensal bacteria that recently emerged as leading causes of antibiotic-resistant, hospital-acquired infection. vancomycin-resistant enterococci (vre) epitomize why drug-resistant enterococcal infections are a problem: vre readily colonize the antibiotic-perturbed gastrointestinal (gi) tract where they amplify to large numbers, and from there, they infect other body sites, including the bloodstream, urinary tract, and surgical wounds. vre are resistant to many antimicrob ... | 2017 | 28223450 |
| determination of antibacterial and technological properties of vaginal lactobacilli for their potential application in dairy products. | functional foods could differently affect human health in relation to the gender. recent studies have highlighted the anti-candida and anti-chlamydia activities of some lactobacillus strains isolated from the vagina of healthy women. considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. in this context, the aim of this work was to study some f ... | 2017 | 28223974 |
| increasing resistance to extended-spectrum cephalosporins, fluoroquinolone, and carbapenem in gram-negative bacilli and the emergence of carbapenem non-susceptibility in klebsiella pneumoniae: analysis of korean antimicrobial resistance monitoring system (karms) data from 2013 to 2015. | national surveillance of antimicrobial resistance becomes more important for the control of antimicrobial resistance and determination of treatment guidelines. we analyzed korean antimicrobial resistance monitoring system (karms) data collected from 2013 to 2015. | 2017 | 28224769 |
| drying and decontamination of raw pistachios with sequential infrared drying, tempering and hot air drying. | pistachio nuts have been associated with outbreaks of foodborne disease and the industry has been impacted by numerous product recalls due to contamination with salmonella enterica. the current hot air drying of pistachios has low energy efficiency and drying rates, and also does not guarantee the microbial safety of products. in the study described herein, dehulled and water-sorted pistachios with a moisture content (mc) of 38.14% (wet basis) were dried in a sequential infrared and hot air (sir ... | 2017 | 28226285 |
| genotypic characterization of vancomycin-resistant enterococcus spp. in tertiary center, iran. | enterococci infection rate, mortality and morbidity has been increased in recent decades accompanied by progressive emerging antimicrobial resistance. vancomycin-resistant enterococci (vre), important pathogen in hospitalized patients, have distinct antibiotic susceptibility to glycopeptides that varied genetically and can influence in choosing therapeutic agents. in this study, we aimed to determine the patterns of antimicrobial resistance according to the genotypic variations in vres. | 2017 | 28228089 |
| development of a rapid identification method for the differentiation of enterococcus species using a species-specific multiplex pcr based on comparative genomics. | enterococci are lactic acid bacteria that are commonly found in food and in animal gut. since 16 s ribosomal rna (rrna) sequences, genetic markers for bacterial identification, are similar among several enterococcus species, it is very difficult to determine the correct species based on only 16 s rrna sequences. therefore, we developed a rapid method for the identification of different enterococcus species using comparative genomics. we compared 38 genomes of 13 enterococcus species retrieved fr ... | 2017 | 28229213 |
| efficacy of rifampicin combination therapy for the treatment of enterococcal infections assessed in vivo using a galleria mellonella infection model. | enterococci are a leading cause of healthcare-associated infection worldwide and display increasing levels of resistance to many of the commonly used antimicrobials, making treatment of their infections challenging. combinations of antibiotics are occasionally employed to treat serious infections, allowing for the possibility of synergistic killing. the aim of this study was to evaluate the effects of different antibacterial combinations against enterococcal isolates using an in vitro approach a ... | 2017 | 28235571 |
| amylolytic enzymes acquired from l-lactic acid producing enterococcus faecium k-1 and improvement of direct lactic acid production from cassava starch. | an amylolytic lactic acid bacterium isolate k-1 was isolated from the wastewater of a cassava starch manufacturing factory and identified as entercoccus faecium based on 16s rrna gene sequence analysis. an extracellular α-amylase was purified to homogeneity and the molecular weight of the purified enzyme was approximately 112 kda with optimal ph value and temperature measured of 7.0 and 40 °c, respectively. it was stable at a ph range of 6.0-7.0, but was markedly sensitive to high temperatures a ... | 2017 | 28236189 |
| enterococcus durans ep1 a promising anti-inflammatory probiotic able to stimulate siga and to increase faecalibacterium prausnitzii abundance. | enterococcus species, principally enterococcus faecium are used as probiotics since a long time with preference in animal applications but safety considerations were updated and also new uses as probiotics can be envisaged. fifteen enterococcus strains isolated from different foods were identified and analyzed for virulence factors and antibiotic resistance. three enterococcus durans strains were selected to study their immunomodulatory properties on pbmc and caco2 cells. two strains presented a ... | 2017 | 28239378 |
| immobilized trienzymatic system with enhanced stabilization for the biotransformation of lactose. | the use of ketohexose isomerases is a powerful tool in lactose whey processing, but these enzymes can be very sensitive and expensive. development of immobilized/stabilized biocatalysts could be a further option to improve the process. in this work, β-galactosidase from bacillus circulans, l-arabinose (d-galactose) isomerase from enterococcus faecium, and d-xylose (d-glucose) isomerase from streptomyces rubiginosus were immobilized individually onto eupergit c and eupergit c 250 l. immobilized a ... | 2017 | 28241449 |
| the two component system chtrs contributes to chlorhexidine tolerance in enterococcus faecium. | enterococcus faecium is one of the primary causes of nosocomial infections. dinsinfectants are commonly used to prevent infections with multidrug-resistant e. faecium in hospitals. worryingly, e. faecium strains that exhibit tolerance to disinfectants have already been described. we aimed to identify and characterize e. faecium genes that contribute to tolerance to the disinfectant chlorhexidine (chx).we used a transposon mutant library, constructed in a multi-drug resistant e. faecium bloodstre ... | 2017 | 28242664 |
| fluorescence high-throughput screening for inhibitors of tonb action. | gram (-) bacteria acquire ferric siderophores through tonb-dependent outer membrane transporters (tbdt). by fluorescence spectroscopic high-throughput screening (flhts) we identified inhibitors of tonb-dependent ferric enterobactin (feent) uptake through e. coli fepa (ecofepa). among 165 inhibitors found in a primary screen of 17,441 compounds, we evaluated 20 in secondary tests: tonb-dependent ferric siderophore uptake and colicin killing; proton-motive force-dependent lactose transport. 6 of 2 ... | 2017 | 28242720 |
| antibacterial nerol cinnamates from the australian plant eremophila longifolia. | two new antimicrobial agents, neryl ferulate (1) and neryl p-coumarate (2), were identified using bioassay-guided isolation from the leaves of eremophila longifolia, which is a medicinal plant used by some australian aboriginal communities. although gradual autoxidation of the nerol subunit hindered the initial attempts to purify and characterize 1 and 2, it was found that the autoxidation could be stopped through storage under argon at -20 °c. biological evaluation showed that neryl ferulate (1 ... | 2017 | 28257200 |
| human norovirus binding to select bacteria representative of the human gut microbiota. | recent reports describe the ability of select bacterial strains to bind human norovirus, although the specificity of such interactions is unknown. the purpose of this work was to determine if a select group of bacterial species representative of human gut microbiota bind to human norovirus, and if so, to characterize the intensity and location of that binding. the bacteria screened included naturally occurring strains isolated from human stool (klebsiella spp., citrobacter spp., bacillus spp., e ... | 2017 | 28257478 |
| antimicrobial-resistant escherichia coli and enterococcus spp. isolated from miranda donkey (equus asinus): an old problem from a new source with a different approach. | the miranda donkey (equus asinus) is an endangeredasinine from miranda do douro region, located in the north east of portugal. we studied the antimicrobial resistance and virulence genes in escherichia coli and enterococcus spp. isolates from these animals. | 2017 | 28260587 |
| infectious anastomotic pseudoaneurysm complicating renal allograft: case report and review of literature. | infectious anastomotic pseudoaneurysm complicating renal transplant is rare, but probably under-reported with <30 cases worldwide. we report a 45-year-old man with hypertension, diabetes mellitus and end stage renal failure, who had a renal transplant anastomosed to the right external iliac artery and vein. postoperatively, he made a slow recovery with malaise and persistent vague right iliac fossa discomfort. ultrasound scan 1 month postoperatively showed perinephric collection, and fluid cultu ... | 2017 | 28260939 |