Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| inflammasome and fas-mediated il-1β contributes to th17/th1 cell induction in pathogenic bacterial infection in vivo. | cd4(+) th cells play crucial roles in orchestrating immune responses against pathogenic microbes, after differentiating into effector subsets. recent research has revealed the importance of ifn-γ and il-17 double-producing cd4(+) th cells, termed th17/th1 cells, in the induction of autoimmune and inflammatory diseases. in addition, th17/th1 cells are involved in the regulation of infection caused by the intracellular bacterium mycobacterium tuberculosis in humans. however, the precise mechanism ... | 2017 | 28674179 |
| comparison of laboratory testing methods for the diagnosis of tuberculous pleurisy in china. | to evaluate the diagnostic utilities of different methods for mycobacterium tuberculosis (m. tuberculosis) detection in pleural fluid that represent potentially cost-effective measures for resource-limited settings in china. we compared diagnostic characteristics of the ampsure simultaneous amplification and testing method, the bactec mgit 960 system, and acid-fast bacilli staining of specimen smears for detection of m. tuberculosis in pleural fluids of 438 patients with suspected tuberculous pl ... | 2017 | 28674427 |
| mycobacterium tuberculosis endocarditis in native valves. | 2017 | 28674587 | |
| genexpert technology. a breakthrough for the diagnosis of tuberculous pericarditis and pleuritis in less than 2 hours. | to evaluate the diagnostic validity of genexpert for the detection of mycobacterium tuberculosis (mtb) in pericardial and pleural effusions samples. | 2017 | 28674714 |
| the sigma factor sigd of mycobacterium tuberculosis putatively enhances gene expression of the septum site determining protein under stressful environments. | this work examined the expression of the septum site determining gene (ssd) of mycobacterium tuberculosis cdc1551 and its δsigd mutant under different growing conditions. the results showed an up-regulation of ssd during stationary phase and starvation conditions, but not during in vitro dormancy, suggesting a putative role for sigd in the control of ssd expression mainly under lack-of-nutrients environments. furthermore, we elucidated a putative link between ssd expression and cell elongation o ... | 2017 | 28675242 |
| plant e3 ligases ubiquitinate escherichia coli σ(32)in vitro. | ubiquitin-like proteins (ubls) are extremely well-conserved among eukaryotes and prokaryotes allowing interactions between proteins from different organisms. for example, the prokaryotic ubiquitin-like proteins (pups) and the proteasome accessory factor a (pafa) of mycobacterium tuberculosis are sufficient to pupylate at least 51 escherichia coli proteins. this work shows that the plant e3 ligases bntr1 and at1g02860 can ubiquitinate e. coli σ(32), but not hsp70 dnak in vitro. molecular biology ... | 2017 | 28676399 |
| hla-e presents glycopeptides from the mycobacterium tuberculosis protein mpt32 to human cd8(+) t cells. | infection with mycobacterium tuberculosis (mtb), the bacterium that causes tuberculosis, remains a global health concern. both classically and non-classically restricted cytotoxic cd8(+) t cells are important to the control of mtb infection. we and others have demonstrated that the non-classical mhc i molecule hla-e can present pathogen-derived peptides to cd8(+) t cells. in this manuscript, we identified the antigen recognized by an hla-e-restricted cd8(+) t cell clone isolated from an mtb late ... | 2017 | 28676677 |
| orbital tuberculosis: clinicopathological correlation and diagnosis using pcr in formalin-fixed tissues. | this article describes the clinicopathological correlation and challenges encountered in the diagnosis of orbital tuberculosis (tb). retrospective, interventional case series. a chart review of six patients who underwent biopsy for an orbital mass between january 2012 and december 2013 was performed. institutional review board approval was obtained for the study. clinical, radiological, and laboratory findings were documented, and response to antitubercular therapy (att) was noted. age at presen ... | 2017 | 28678592 |
| mechanism-based inactivator of isocitrate lyases 1 and 2 from mycobacterium tuberculosis. | isocitrate lyase (icl, types 1 and 2) is the first enzyme of the glyoxylate shunt, an essential pathway for mycobacterium tuberculosis (mtb) during the persistent phase of human tb infection. here, we report 2-vinyl-d-isocitrate (2-vic) as a mechanism-based inactivator of mtb icl1 and icl2. the enzyme-catalyzed retro-aldol cleavage of 2-vic unmasks a michael substrate, 2-vinylglyoxylate, which then forms a slowly reversible, covalent adduct with the thiolate form of active-site cys191 2-vic disp ... | 2017 | 28679637 |
| virtual screening, pharmacophore development and structure based similarity search to identify inhibitors against ider, a transcription factor of mycobacterium tuberculosis. | ider, an essential gene of mycobacterium tuberculosis, is an attractive drug target as its conditional knockout displayed attenuated growth phenotype in vitro and in vivo. to the best of our knowledge, no inhibitors of ider are identified. we carried out virtual screening of nci database against the ider dna binding domain followed by inhibition studies using emsa. nine compounds exhibited potent inhibition with nsc 281033 (i-20) and nsc 12453 (i-42) exhibiting ic50 values of 2 µg/ml and 1 µg/ml ... | 2017 | 28680150 |
| disruption of key nadh-binding pocket residues of the mycobacterium tuberculosis inha affects dd-coa binding ability. | tuberculosis (tb) is a global health problem that affects over 10 million people. there is an urgent need to develop novel antimicrobial therapies to combat tb. to achieve this, a thorough understanding of key validated drug targets is required. the enoyl reductase inha, responsible for synthesis of essential mycolic acids in the mycobacterial cell wall, is the target for the frontline anti-tb drug isoniazid. to better understand the activity of this protein a series of mutants, targeted to the ... | 2017 | 28680153 |
| robust immunoreactivity of teenager sera against peptide 19 from porphyromonas gingivalis hsp60. | epitope spreading is a phenomenon in which distinct subdominant epitopes become major targets of the immune response. heat shock protein (hsp) 60 from porphyromonas gingivalis (pghsp60) and peptide 19 from pghsp60 (pep19) are immunodominant epitopes in autoimmune disease patients, including those with periodontitis. it remains unclear whether pep19 is a dominant epitope in subjects without periodontitis or autoimmune disease. the purpose of this study was to determine the epitope spreading patte ... | 2017 | 28680713 |
| genotypic characterisation of mycobacterium tuberculosis isolates from tuberculous meningitis patients at a tertiary neurocare centre in southern india. | specific genotypes of mycobacterium tuberculosis (mtb) have been reported to cause outbreaks of pulmonary tuberculosis (tb) in geographical areas that are endemic to tb. however, since there is little epidemiological evidence on the association of particular genotypes that cause tuberculous meningitis (tbm), we sought to investigate the association of specific mtb strains with infection of the central nervous system (cns). | 2017 | 28681808 |
| levels of circulating immune complexes containing mycobacterium tuberculosis-specific antigens in pulmonary tuberculosis and sarcoidosis patients. | the present study was conducted to understand the aetiological link between tuberculosis (tb) and sarcoidosis. sera from smear-positive tb subjects (n = 24), smear-negative tb subjects (n = 24), sarcoidosis patients (n = 24) and healthy controls (n = 24) were collected and circulating immune complexes were isolated. sandwich elisa was performed for detecting four highly specific mycobacterial regions of difference (rd) proteins (early secretory antigenic target 6 [esat6], 10 kda culture filtrate ... | 2017 | 28681824 |
| development and evaluation of a loop-mediated isothermal amplification combined with au-nanoprobe assay for rapid detection of mycobacterium tuberculosis. | loop-mediated isothermal amplification (lamp) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. we present a novel strategy for the detection of lamp amplicons derived from mycobacterium tuberculosis by the use of au-nanoprobes. when applied to a total of 93 clinical specimens, the lamp assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. the au-nanoprobe augmented lamp test platform with its advantages o ... | 2017 | 28681828 |
| synthesis of a 3-amino-2,3-dihydropyrid-4-one and related heterocyclic analogues as mechanism-based inhibitors of bioa, a pyridoxal phosphate-dependent enzyme. | amiclenomycin (acm) is a chemically unstable antibiotic with selective activity against mycobacterium tuberculosis (mtb) due to mechanism-based inhibition of bioa, a pyridoxal 5'-phosphate (plp)-dependent aminotransferase. the first-generation acm analogue dihydro-2-pyridone 1 maintains a similar bioactivation mechanism concluding with covalent labeling of the plp cofactor. to improve on 1, we report the synthesis of dihydro-4-pyranone 2, dihydro-4-pyridone 3, and dihydro-4-thiopyranone 13, whic ... | 2017 | 28682613 |
| synthesis and immunological studies of the lipomannan backbone glycans found on the surface of mycobacterium tuberculosis. | investigations into novel bacterial drug targets and vaccines are necessary to overcome tuberculosis. lipomannan (lm), found on the surface of mycobacterium tuberculosis (mtb), is actively involved in the pathogenesis and survival of mtb. here, we report for the first time a rapid synthesis and biological activities of an lm glycan backbone, α(1-6)mannans. the rapid synthesis is achieved via a regio- and stereoselective ring opening polymerization to generate multiple glycosidic bonds in one sim ... | 2017 | 28682637 |
| proteomics of culture filtrate of prevalent mycobacterium tuberculosis strains: 2d-page map and maldi-tof/ms analysis. | although diverse efforts have been done to identify biomarkers for control of tuberculosis using laboratory strain mycobacterium tuberculosis h37rv, the disease still poses a threat to mankind. there are many emerging m. tuberculosis strains, and proteomic profiling of these strains might be important to find out potential targets for diagnosis and/or prevention of tuberculosis. we evaluated the comparative proteomic profiling of culture filtrate (cf) proteins from prevalent m. tuberculosis stra ... | 2017 | 28683213 |
| the complete structure of the mycobacterium smegmatis 70s ribosome. | the ribosome carries out the synthesis of proteins in every living cell. it consequently represents a frontline target in anti-microbial therapy. tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. here, we present the 3.3-å cryo-em structure of the 70s ribosome of mycobacterium smegmatis, a close relative to the human pathogen mycobacterium tuberculosis. the structure reveals two additional ... | 2017 | 28683309 |
| host-directed therapeutics as novel angle for tuberculosis treatment. | despite significant efforts to improve tuberculosis (tb) treatment, it remains a worldwide infectious disease due to the limitations of current tb regimens. recent work on novel tb treatment strategies has suggested that directly targeting host factors is beneficial for tb treatment. such strategies, termed host-directed therapeutics (hdts), focus on host-pathogen interactions. hdts are more beneficial than existed tb drugs, which are limited the long durations of treatment and the emergence of ... | 2017 | 28683527 |
| longitudinal profiling reveals a persistent intestinal dysbiosis triggered by conventional anti-tuberculosis therapy. | effective treatment of mycobacterium tuberculosis (mtb) infection requires at least 6 months of daily therapy with multiple orally administered antibiotics. although this drug regimen is administered annually to millions worldwide, the impact of such intensive antimicrobial treatment on the host microbiome has never been formally investigated. here, we characterized the longitudinal outcome of conventional isoniazid-rifampin-pyrazinamide (hrz) tb drug administration on the diversity and composit ... | 2017 | 28683818 |
| complete genome sequences of three representative mycobacterium tuberculosis beijing family strains belonging to distinct genotype clusters in hanoi, vietnam, during 2007 to 2009. | we present here three complete genome sequences of mycobacterium tuberculosis beijing family strains isolated in hanoi, vietnam. these three strains were selected from major genotypic clusters (15-miru-vntr) identified in a previous population-based study. we emphasize their importance and potential as reference strains in this asian region. | 2017 | 28684565 |
| erratum: data intensive genome level analysis for identifying novel, non-toxic drug targets for multi drug resistant mycobacterium tuberculosis. | this corrects the article doi: 10.1038/srep46595. | 2017 | 28684876 |
| evaluation of mycotube, a modified version of lowenstein-jensen (lj) medium, for efficient recovery of mycobacterium tuberculosis (mtb). | timely diagnosis of tuberculosis (tb), caused by mycobacterium tuberculosis (mtb), is only achieved for ~58% cases. an improved, accurate, time- and cost-effective method for bacteriological confirmation of mtb is necessary. we evaluated mycotube, a new variant of lowenstein-jensen (lj) culture medium, by comparing it with mycobacterium growth indicator tube (mgit) 960 (gold standard), local lj, and biomérieux lj-t in terms of isolation rate and time-to-growth. pulmonary and extra-pulmonary samp ... | 2017 | 28685188 |
| conjugated and entrapped hpma-pla nano-polymeric micelles based dual delivery of first line anti tb drugs: improved and safe drug delivery against sensitive and resistant mycobacterium tuberculosis. | first line antitb drugs have several physical and toxic manifestations which limit their applications. rif is a hydrophobic drug and has low water solubility and inh is hepatotoxic. the main objective of the study was to synthesize, characterize hpma-pla co-polymeric micelles for the effective dual delivery of inh and rif. | 2017 | 28685299 |
| human genetic factors in tuberculosis: an update. | tuberculosis (tb) is a major threat to human health, especially in many developing countries. human genetic variability has been recognised to be of great relevance in host responses to mycobacterium tuberculosis infection and in regulating both the establishment and the progression of the disease. an increasing number of candidate gene and genome-wide association studies (gwas) have focused on human genetic factors contributing to susceptibility or resistance to tb. to update previous reviews o ... | 2017 | 28685916 |
| proteomic profile of mycobacterium tuberculosis after eupomatenoid-5 induction reveals potential drug targets. | we investigated a proteome profile, protein-protein interaction and morphological changes of mycobacterium tuberculosis after different times of eupomatenoid-5 (eup-5) induction to evaluate the cellular response to the drug-induced damages. | 2017 | 28686056 |
| drug resistance profiles of new- and previously treated patients with pulmonary tuberculosis in ibadan, nigeria. | information on tb drug resistance profiles and its' associated risk factors are scarce in nigeria despite the large burden of disease in the country. the study was designed to report drug resistance profiles of new- and previously treated patients with pulmonary tuberculosis (tb) in ibadan, nigeria. | 2016 | 28686829 |
| a review of eleven cases of tuberculosis presenting as sternal wound abscess after open heart surgery. | sternal wound infection with mycobacterium tuberculosis is an uncommon yet highly challenging disease that can be quite insidious with various presentations. we hereby provide a review of 10 cases in current literature and describe an additional case which illustrates the difficulties associated with diagnosis. | 2017 | 28687049 |
| invasive mucinous adenocarcinoma with lepidic-predominant pattern coexisted with tuberculosis: a case report. | we observed a rare case of invasive mucinous adenocarcinoma (ima) with a lepidic-predominant pattern accompanied by pulmonary tuberculosis. an 85-year-old man with repeated cough and sputum was admitted to xinhua hospital. t-spot test result was 212 pg/ml (reference value of negative is < 14 pg/ml), mycobacterium tuberculosis culture was positive, and tuberculin skin test (ppd) was negative (skin induration < 5 mm). the patient was treated with several courses of antibiotics and anti-tuberculosi ... | 2017 | 28687977 |
| survival of mycobacterium tuberculosis and mycobacterium bovis bcg in lysosomes in vivo. | mycobacterium tuberculosis is one of the most successful pathogens known, having infected more than a third of the global population. an important strategy for intracellular survival of pathogenic mycobacteria relies on their capacity to resist delivery to lysosomes, instead surviving within macrophage phagosomes. several factors of both mycobacterial and host origin have been implicated in this process. however, whether or not this strategy is employed in vivo is not clear. here we show that in ... | 2017 | 28689009 |
| design, synthesis and antitubercular potency of 4-hydroxyquinolin-2(1h)-ones. | in this study, a 50-membered library of substituted 4-hydroxyquinolin-2(1h)-ones and two closely related analogues was designed, scored in-silico for drug likeness and subsequently synthesized. thirteen derivatives, all sharing a common 3-phenyl substituent showed minimal inhibitory concentrations against mycobacterium tuberculosis h37ra below 10 μm and against mycobacterium bovis an5a below 15 μm but were inactive against faster growing mycobacterial species. none of these selected derivatives ... | 2017 | 28689097 |
| selection of a new mycobacterium tuberculosis h37rv aptamer and its application in the construction of a swcnt/aptamer/au-ide mspqc h37rv sensor. | a rapid and accurate detection method for mycobacterium tuberculosis (m. tuberculosis) is essential for effectively treating tuberculosis. however, current detection methods cannot meet these clinical requirements because the methods are slow or of low specificity. consequently, a new highly specific ssdna aptamer against m. tuberculosis reference strain h37rv was selected by using the whole-cell systematic evolution of ligands by exponential enrichment technique. the selected aptamer was used t ... | 2017 | 28689112 |
| combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis. | early diagnosis of tuberculosis is of major clinical importance. among 4733 clinical specimens collected from 3363 patients and subjected to ziehl-neelsen microscopy, 4109 were inoculated onto löwenstein-jensen slants and 3139 in bactec/9000mb. polymerase chain reaction (pcr) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 mycobacterium tuberculosis complex pcr-positive for isoniazid and rifampicin resistance detection specimens (genotype mtbdrplus). recove ... | 2017 | 28689813 |
| [diagnostic value of different detection methods for mycobacterium tuberculosis in bronchoalveolar lavage fluid from pulmonary tuberculosis patients]. | to investigate the diagnostic value of different detection methods for mycobacterium tuberculosis in bronchoalveolar lavage fluid (balf) from patients with pulmonary tuberculosis. methods: balf from100 patients in changsha central hospital from january 2013 to december 2015 was collected. among 100 patients, 65 cases were clinically diagnosed as tuberculosis, and 35 cases served as control. balf smear method, polymerase chain reaction (pcr) and membrane reverse dot blot (rdb) were used for sync ... | 2017 | 28690221 |
| identification of inhibitors against α-isopropylmalate synthase of mycobacterium tuberculosis using docking-mm/pbsa hybrid approach. | α-isopropylmalate synthase (α-ipms) encoded by leua in mycobacterium tuberculosis (m.tb) is involved in the leucine biosynthesis pathway and is extremely critical for the synthesis of branched-chain amino acids (leucine, isoleucine and valine). α-ipms activity is required not only for the proliferation of m.tb but is also indispensable for its survival during the latent phase of infection. it is absent in humans and is widely regarded as one of the validated drug targets against tuberculosis (tb ... | 2017 | 28690380 |
| case report of a lupus patient with a severe flare and miliary tuberculosis: need for proper guidelines for management. | systemic lupus erythematosus (sle) is an autoimmune disease that is frequently treated with high doses of corticosteroids and other immunosuppressive drugs. thus patients with sle are at increased risk for infections with several pathogens including mycobacterium tuberculosis. there are no established guidelines available for treatment of tuberculosis in sle patients with high disease activity due to lack of relevant studies and management based more on physician expertise. we report a case of a ... | 2017 | 28690859 |
| the emergence of phenolic glycans as virulence factors in mycobacterium tuberculosis. | tuberculosis is the leading infectious cause of mortality worldwide. the global epidemic, caused by mycobacterium tuberculosis, has prompted renewed interest in the development of novel vaccines for disease prevention and control. the cell envelope of m. tuberculosis is decorated with an assortment of glycan structures, including glycolipids, that are involved in disease pathogenesis. phenolic glycolipids and the structurally related para-hydroxybenzoic acid derivatives display potent immunomodu ... | 2017 | 28692249 |
| structural and biophysical characterization of the mycobacterium tuberculosis protein rv0577, a protein associated with neutral red staining of virulent tuberculosis strains and homologue of the streptomyces coelicolor protein kbpa. | mycobacterium tuberculosis protein rv0577 is a prominent antigen in tuberculosis patients, the component responsible for neutral red staining of virulent strains of m. tuberculosis, a putative component in a methylglyoxal detoxification pathway, and an agonist of toll-like receptor 2. it also has an amino acid sequence that is 36% identical to that of streptomyces coelicolor afsk-binding protein a (kbpa), a component in the complex secondary metabolite pathways in the streptomyces genus. to gain ... | 2017 | 28692281 |
| whole genome sequencing of mycobacterium tuberculosis isolates from extrapulmonary sites. | tuberculosis (tb) remains one of the leading causes of morbidity and mortality worldwide. extrapulmonary tuberculosis (eptb) constitutes around 15-20% of tb cases in immunocompetent individuals. extrapulmonary sites that are affected by tb include bones, lymph nodes, meningitis, pleura, and genitourinary tract. whole genome sequencing has emerged as a powerful tool to map genetic diversity among mycobacterium tuberculosis (mtb) isolates and identify the genomic signatures associated with drug re ... | 2017 | 28692415 |
| evaluation of the relationship between marco and cd36 single-nucleotide polymorphisms and susceptibility to pulmonary tuberculosis in a chinese han population. | gene polymorphisms impact greatly on a person's susceptibility to pulmonary tuberculosis (ptb). macrophage receptor with collagenous structure (marco) and cd36 are two scavenger receptors (srs) that can recognize mycobacterium tuberculosis (mtb) and play a key role in tuberculosis infection. gene polymorphisms of marco and cd36 may contribute to tuberculosis risk. | 2017 | 28693442 |
| hydrophilic interaction liquid chromatography-mass spectrometry as a new tool for the characterization of intact semi-synthetic glycoproteins. | improved methods for detailed characterization of complex glycoproteins are required in the growing sector of biopharmaceuticals. hydrophilic interaction liquid chromatography (hilic) coupled to high resolution (hr) time-of-flight mass spectrometric (tof-ms) detection was examined for the characterization of intact neo-glycoproteins prepared by chemical conjugation of synthetic saccharides to the lysine residues of selected recombinant proteins. the separation performances of three different ami ... | 2017 | 28693734 |
| systematic review and meta-analysis of the nitrate reductase assay for drug susceptibility testing of mycobacterium tuberculosis and the detection limits in liquid medium. | recently, the need for rapid, reliable, and low-cost drug susceptibility testing (dst) methods has increased due to the emergence of multidrug-resistant mycobacterium tuberculosis. colorimetric methods of dst provide results more quickly than standard culture methods and are inexpensive than molecular methods. thus, colorimetric methods, such as the nitrate reductase assay (nra), are being recommended. we searched medline pubmed for reports on the nra for dst of m. tuberculosis written in englis ... | 2017 | 28694139 |
| medicinal plants used in the treatment of tuberculosis - ethnobotanical and ethnopharmacological approaches. | tuberculosis is a highly infectious disease declared a global health emergency by the world health organization, with approximately one third of the world's population being latently infected with mycobacterium tuberculosis. tuberculosis treatment consists in an intensive phase and a continuation phase. unfortunately, the appearance of multi drug-resistant tuberculosis, mainly due to low adherence to prescribed therapies or inefficient healthcare structures, requires at least 20months of treatme ... | 2017 | 28694178 |
| mycobacterium tuberculosis dosr regulon gene rv2004c encodes a novel antigen with pro-inflammatory functions and potential diagnostic application for detection of latent tuberculosis. | approximately 1.7 billion people in the world harbor latent mycobacterium tuberculosis (mtb) with a substantial risk of progression to clinical outcome. containment of these seed beds of mtb is essential to eliminate tuberculosis completely in high burden settings such as india. hence, there is an urgent need for the identification of new serological markers for detection or vaccine candidates to prevent latent tuberculosis infection (ltbi). dosr regulon antigens of mtb might serve as attractive ... | 2017 | 28694808 |
| an uncommon cause of longitudinally extensive transverse myelitis. | considering that currently romania has the highest tuberculosis incidence in europe, the recognition of the infection is an acknowledged health issue. central nervous system tuberculosis accounts for approximately 1% of all cases of tuberculosis. longitudinally extensive transverse myelitis is a contiguous infl ammatory lesion of the spinal cord which involves three or more spinal segments. in tuberculosis, spinal cord involvement is rare and is usually rather due to radiculomyelitis or spinal t ... | 2016 | 28694861 |
| abdominal tuberculosis after removal of an adjustable gastric band - report of an unusual case. | laparoscopic adjustable gastric banding (lagb) is the third most popular bariatric procedure. despite its reversibility and minimal invasiveness, band infection affects 1.2% of patients. we present a case of a 25-year-old, obese woman who was experiencing malaise and feverishness 3 years after gastric band placement. due to port site infection the port was removed, which did not improve the patient's condition. after 2 years the band was removed via laparotomy with a minor surgical site infectio ... | 2017 | 28694906 |
| the cording phenotype of mycobacterium tuberculosis induces the formation of extracellular traps in human macrophages. | the causative agent of tuberculosis, mycobacterium tuberculosis, shares several characteristics with organisms that produce biofilms during infections. one of these is the ability to form tight bundles also known as cords. however, little is known of the physiological relevance of the cording phenotype. in this study, we investigated whether cord-forming m. tuberculosis induce the formation of macrophage extracellular traps (mets) in human monocyte-derived macrophages. macrophages have previousl ... | 2017 | 28695112 |
| the putative polyketide cyclase msmeg_0129 from mycobacterium smegmatis: purification, crystallization and x-ray crystallographic analysis. | mycobacterium tuberculosis rv0164 has previously been identified as a human t-cell antigen that induces significant production of ifn-γ in human peripheral blood mononuclear cells. m. smegmatis msmeg_0129 shares 59% sequence identity with rv0164. based on sequence alignment, both proteins are predicted to be members of the cyclase/dehydrase family, which is part of a large group of enzymes referred to as type ii polyketide synthases (pkss). in biosynthetic pathways mediated by type ii pkss, cycl ... | 2017 | 28695854 |
| innate scavenger receptor-a regulates adaptive t helper cell responses to pathogen infection. | the pattern recognition receptor (prr) scavenger receptor class a (sr-a) has an important function in the pathogenesis of non-infectious diseases and in innate immune responses to pathogen infections. however, little is known about the role of sr-a in the host adaptive immune responses to pathogen infection. here we show with mouse models of helminth schistosoma japonicum infection and heat-inactivated mycobacterium tuberculosis stimulation that sr-a is regulated by pathogens and suppresses irf5 ... | 2017 | 28695899 |
| sterilizing effect of ertapenem-clavulanate in a hollow-fiber model of tuberculosis and implications on clinical dosing. | carbapenems are now being explored for treatment of multidrug-resistant tuberculosis (mdr-tb), especially in conjunction with clavulanate. clinical use is constrained by the need for multiple parenteral doses per day and the lack of knowledge of the optimal dose for sterilizing effect. our objective was to identify the ertapenem exposure associated with optimal sterilizing effect and then design a once-a-day dose for clinical use. we utilized the hollow-fiber system model of tuberculosis in a 28 ... | 2017 | 28696238 |
| ethambutol partitioning in tuberculous pulmonary lesions explains its clinical efficacy. | clinical trials and practice have shown that ethambutol is an important component of the first-line tuberculosis (tb) regime. this contrasts the drug's rather modest potency and lack of activity against nongrowing persister mycobacteria. the standard plasma-based pharmacokinetic-pharmacodynamic profile of ethambutol suggests that the drug may be of limited clinical value. here, we hypothesized that this apparent contradiction may be explained by favorable penetration of the drug into tb lesions. ... | 2017 | 28696241 |
| defining the functionally important domain and amino acid residues in mycobacterium tuberculosis integration host factor for genome stability, dna binding and integrative recombination. | the integration host factor of mycobacterium tuberculosis (mihf) consists of a single polypeptide chain, the product of the ihf gene. we previously revealed that mihf is a novel member of a new class of nucleoid-associated proteins that have important roles in dna damage response, nucleoid compaction and integrative recombination. the mihf contains a region of 86 amino acids at its n-terminus, absent in both α- and β-subunits of escherichia coli ihf. however, the functional significance of an ex ... | 2017 | 28696279 |
| plumbago auriculata leaf extract-mediated agnps and its activities as antioxidant, anti-tb and dye degrading agents. | in the present work, silver nanoparticles have been biosynthesized by utilizing the alcoholic extract of plumbago auriculata. the optimization of reaction conditions was carried out by monitoring the reactions with the help of uv-visible absorption spectroscopy. the characterization of agnp was carried out by infrared spectroscopy, transmission electron microscopy and x-ray diffraction (xrd) studies. the biogenic agnps were tested against mycobacterium tuberculosis using microplate almar blue as ... | 2017 | 28697688 |
| pyrazinamide resistance-conferring mutations in pnca and the transmission of multidrug resistant tb in georgia. | the ongoing epidemic of multidrug-resistant tuberculosis (mdr-tb) in georgia highlights the need for more effective control strategies. a new regimen to treat mdr-tb that includes pyrazinamide (pza) is currently being evaluated and pza resistance status will largely influence the success of current and future treatment strategies. pza susceptibility testing was not routinely performed at the national reference laboratory (nrl) in tbilisi between 2010 and september 2015. we here provide a first i ... | 2017 | 28697808 |
| multidrug-resistant/extensively drug-resistant tuberculosis in greece: predominance of mycobacterium tuberculosis genotypes endemic in the former soviet union countries. | 2017 | 28698038 | |
| mycobacterium tuberculosis phoy proteins promote persister formation by mediating pst/senx3-regx3 phosphate sensing. | the mycobacterium tuberculosis phosphate-specific transport (pst) system controls gene expression in response to phosphate availability by inhibiting the activation of the senx3-regx3 two-component system under phosphate-rich conditions, but the mechanism of communication between these systems is unknown. in escherichia coli, inhibition of the two-component system phor-phob under phosphate-rich conditions requires both the pst system and phou, a putative adaptor protein. e. coli phou is also inv ... | 2017 | 28698272 |
| a novel protein kinase inhibitor imb-yh-8 with anti-tuberculosis activity. | protein kinase b (pknb) is one of the mycobacterium tuberculosis serine/threonine protein kinases and has an essential role in sustaining mycobacterial growth. here, we identified and characterized a novel small molecule compound named imb-yh-8 that inhibited pknb and served as anti-mycobacteria lead compound. imb-yh-8 inhibited pknb auto-phosphorylation and the phosphorylation of gara by pknb in a dose-dependent manner. the compound did not inhibit human akt1 or other serine/threonine kinases i ... | 2017 | 28698545 |
| antigens rv0310c and rv1255c are promising novel biomarkers for the diagnosis of mycobacterium tuberculosis infection. | this study aimed to identify novel immunogenic epitopes from mycobacterium tuberculosis (mtb) that could be used in tuberculosis (tb) diagnostics. to determine the diagnostic potential of mycobacterial antigens in serodiagnosis of tb, 256 patients were enrolled in a study and divided into two groups: 126 smear-positive pulmonary tb patients (sppt) and 130 smear-negative pulmonary tb patients (snpt); 152 bacillus calmette-guerin (bcg)-vaccinated healthy people were used as a control. murine resul ... | 2017 | 28698665 |
| tuberculosis in kidney transplant recipients: a case series. | solid organ transplant recipients have an elevated risk of tuberculosis (tb) with high mortality. data about tb in this population in the united states is sparse. we present four cases of active tuberculosis in kidney transplant recipients at our center. all patients had possible tb exposure prior to transplant and all were diagnosed with active tb within the first year of transplant. disseminated tb was seen in half of the patients with extra-pulmonary tb being more common affecting lymph nodes ... | 2017 | 28698838 |
| antituberculotic activity of actinobacteria isolated from the rare habitats. | a distinctive screening procedure resulted in the isolation and identification of antituberculotic actinobacteria. in this course, a total of 125 actinobacteria were isolated from various soil samples from untapped areas in northwestern himalayas, india. the antibacterial screening showed that 26 isolates inhibited the growth of at least one of the tested bacterial pathogens including staphylococcus aureus (atcc 25923), staphylococcus epidermidis (atcc 12228), bacillus subtilis (atcc 11774), mic ... | 2017 | 28699251 |
| cell wall associated factors of mycobacterium tuberculosis as major virulence determinants: current perspectives in drugs discovery and design. | mycobacteria genus is responsible for deadly diseases like tuberculosis and leprosy. cell wall of bacteria belonging to this genus is unique in many ways. it plays a major role in pathogenesis and intracellular survival inside the host. in intracellular pathogens, their cell wall acts as molecular shield and interacts with host cell milieu to modulate host defense responses. unique role of cell wall associated factors that play crucial roles in the biosynthesis of mycobacterial cell wall compone ... | 2017 | 28699515 |
| fermentation, isolation, purification and characterization of an antitubercular antibiotic from streptomyces luridus mtcc 4402. | tuberculosis, caused by mycobacterium tuberculosis, continues to be a serious public health problem around the world, and it urges the need for development of new antitubercular drugs. an antibiotic producing strain, streptomyces luridus (mtcc 4402) was earlier isolated from soil by our group. in this work, the phylogenic status was established by 16s rrna gene sequence analysis. the strain was found to be active against clinically resistant strains. the culture was grown in shake flasks in a me ... | 2016 | 28699723 |
| in vitro synergistic effects of a short cationic peptide and clinically used antibiotics against drug-resistant isolates of brucella melitensis. | in the last few decades, increasing microbial resistance to common antibiotics has attracted researchers' attention to the development of new classes of antibiotics such as antimicrobial peptides. accordingly, the aim of the current study was to evaluate antimicrobial effects of the cm11 peptide alone and combined with common antibiotics against drug-resistant isolates of brucella melitensis. | 2017 | 28699872 |
| the conserved actinobacterial two-component system mtrab coordinates chloramphenicol production with sporulation in streptomyces venezuelae nrrl b-65442. | streptomyces bacteria make numerous secondary metabolites, including half of all known antibiotics. production of antibiotics is usually coordinated with the onset of sporulation but the cross regulation of these processes is not fully understood. this is important because most streptomyces antibiotics are produced at low levels or not at all under laboratory conditions and this makes large scale production of these compounds very challenging. here, we characterize the highly conserved actinobac ... | 2017 | 28702006 |
| novel vaccination approaches to prevent tuberculosis in children. | pediatric tuberculosis (tb) is an underappreciated problem and accounts for 10 % of all tb deaths worldwide. children are highly susceptible to infection with mycobacterium tuberculosis and interrupting tb spread would require the development of effective strategies to control tb transmission in pediatric populations. the current vaccine for tb, m. bovis bacille calmette-guérin (bcg), can afford some level of protection against tb meningitis and severe forms of disseminated tb in children; howev ... | 2016 | 28702297 |
| tuberculosis and pneumonia in hiv-infected children: an overview. | pneumonia remains the most common cause of hospitalization and the most important cause of death in young children. in high human immunodeficiency virus (hiv)-burden settings, hiv-infected children carry a high burden of lower respiratory tract infection from common respiratory viruses, bacteria and mycobacterium tuberculosis. in addition, pneumocystis jirovecii and cytomegalovirus are important opportunistic pathogens. as the vertical transmission risk of hiv decreases and access to antiretrovi ... | 2016 | 28702298 |
| mutagenesis of threonine to serine in the active site of mycobacterium tuberculosis fructose-1,6-bisphosphatase (class ii) retains partial enzyme activity. | the glpx gene encodes for the class ii fructose-1,6-bisphosphatase enzyme in mycobacterium tuberculosis (mt), an essential enzyme for pathogenesis. we have performed site directed mutagenesis to introduce two mutations at residue thr84, t84a and t84s, to explore the binding affinity of the substrate and the catalytic mechanism. the t84a mutant fully abolishes enzyme activity while retaining substrate binding affinity. in contrast, the t84s mutant retains some activity having a 10 times reduction ... | 2017 | 28702369 |
| the role of human host genetics in tuberculosis resistance. | tuberculosis (tb) remains a public health problem: the latest estimate of new incident cases per year is a staggering 10.4 million. despite this overwhelming number, the majority of the immunocompetent population can control infection with mycobacterium tuberculosis. the human genome underlies the immune response and contributes to the outcome of tb infection. areas covered: investigations of tb resistance in the general population have closely mirrored those of other infectious diseases and ini ... | 2017 | 28703045 |
| synthesis and in vitro antimycobacterial and antibacterial activity of 8-ome ciprofloxacin-hydrozone/azole hybrids. | a series of novel 8-ome ciprofloxacin (cpfx)-hydrazone/azole hybrids were designed, synthesized, and evaluated for their in vitro biological activities. our results reveal that all of the hydrozone-containing hybrids (except for 7) show potency against mycobacterium tuberculosis (mtb) h37rv (minimum inhibitory concentration (mic): <0.5 μm), which is better than the parent drug cpfx, and comparable to moxifloxacin and isoniazid, some of the tested gram-positive strains (mic: 0.06-4 μg/ml), and mo ... | 2017 | 28703766 |
| reduced susceptibility of clinical strains of mycobacterium tuberculosis to reactive nitrogen species promotes survival in activated macrophages. | drugs such as isoniazid (inh) and pretomanid (prt), used against mycobacterium tuberculosis are active partly through generation of reactive nitrogen species (rns). the aim of this study was to explore variability in intracellular susceptibility to nitric oxide (no) in clinical strains of m. tuberculosis. | 2017 | 28704501 |
| phenotypic and genomic comparison of mycobacterium aurum and surrogate model species to mycobacterium tuberculosis: implications for drug discovery. | tuberculosis (tb) is caused by mycobacterium tuberculosis and represents one of the major challenges facing drug discovery initiatives worldwide. the considerable rise in bacterial drug resistance in recent years has led to the need of new drugs and drug regimens. model systems are regularly used to speed-up the drug discovery process and circumvent biosafety issues associated with manipulating m. tuberculosis. these include the use of strains such as mycobacterium smegmatis and mycobacterium ma ... | 2017 | 28705154 |
| reactogenicity to major tuberculosis antigens absent in bcg is linked to improved protection against mycobacterium tuberculosis. | mtbvac is a live-attenuated mycobacterium tuberculosis vaccine, currently under clinical development, that contains the major antigens esat6 and cfp10. these antigens are absent from the current tuberculosis vaccine, bcg. here we compare the protection induced by bcg and mtbvac in several mouse strains that naturally express different mhc haplotypes differentially recognizing esat6 and cfp10. mtbvac induces improved protection in c3h mice, the only of the three tested strains reactive to both es ... | 2017 | 28706226 |
| host sirtuin 1 regulates mycobacterial immunopathogenesis and represents a therapeutic target against tuberculosis. | mycobacterium tuberculosis (mtb) executes a plethora of immune-evasive mechanisms, which contribute to its pathogenesis, limited efficacy of current therapy, and the emergence of drug-resistant strains. this has led to resurgence in attempts to develop new therapeutic strategies/targets against tuberculosis (tb). we show that mtb down-regulates sirtuin 1 (sirt1), a nicotinamide adenine dinucleotide (nad(+))-dependent deacetylase, in monocytes/macrophages, tb animal models, and tb patients with a ... | 2017 | 28707004 |
| misdiagnosis of tuberculosis associated with some species of nontuberculous mycobacteria by genexpert mtb/rif assay. | out of 12 nontuberculous mycobacteria (ntm) species, 5 were identified as mycobacterium tuberculosis (mtb) by genexpert at a bacterial load of 10(6). notably, two species, including m. abscessus and m. smegmatis, were flagged as rif-resistant mtb due to the high c(t) value of probe e. in conclusion, our data have demonstrated that the misdiagnosis of mtb by genexpert assay is observed in five ntm species at a high bacterial load. | 2017 | 28707184 |
| a review of recent advancements in anti-tubercular molecular hybrids. | tuberculosis (tb) accounts for millions of deaths worldwide every year. diverse survival strategies adopted by mycobacterium tuberculosis (mtb) has substantially hindered the existing anti-tb regimen thereby leading to multidrug-resistant (mdr) and extremely drug-resistant (xdr) strains of tb. the steady decrease in current antibiotics' efficacy against these adversities is an indicator that their adequate replenishment in future is almost impossible, placing society on the precipice of a catast ... | 2017 | 28707584 |
| protein tagging, destruction and infection. | cells possess protein quality control mechanisms to maintain proper cellular homeostasis. in eukaryotes, the roles of the ubiquitination and proteasome-mediated degradation of cellular proteins is well established. recent studies have elucidated protein tagging mechanisms in prokaryotes, involving transfer messenger rna (tmrna) and pupylation. in this review, newer insights and bioinformatics analysis of two distinct bacterial protein tagging machineries are discussed. the machinery for tmrna-me ... | 2017 | 28707598 |
| rapid diagnosis of extrapulmonary tuberculosis with xpert mycobacterium tuberculosis/rifampicin assay. | xpertmycobacterium tuberculosis/rifampicin (xpert mtb/rif) assay has been endorsed by the world health organization (who) for diagnosis of extrapulmonary tuberculosis (eptb), while the sensitivity and specificity have not been fully evaluated. we aimed to evaluate the performance of xpert mtb/rif assay in the detection of different extrapulmonary specimens. | 2017 | 28708053 |
| correction: mycobacterium tuberculosis arrests host cycle at the g1/s transition to establish long term infection. | [this corrects the article doi: 10.1371/journal.ppat.1006389.]. | 2017 | 28708888 |
| rv3723/luca coordinates fatty acid and cholesterol uptake in mycobacterium tuberculosis. | pathogenic bacteria have evolved highly specialized systems to extract essential nutrients from their hosts. mycobacterium tuberculosis (mtb) scavenges lipids (cholesterol and fatty acids) to maintain infections in mammals but mechanisms and proteins responsible for the import of fatty acids in mtb were previously unknown. here, we identify and determine that the previously uncharacterized protein rv3723/luca, functions to integrate cholesterol and fatty acid uptake in mtb. rv3723/luca interacts ... | 2017 | 28708968 |
| parotid tuberculosis. | parotid gland tuberculosis is an uncommon manifestation of one of the most common infections even in the developing countries, caused by mycobacterium tuberculosis. there are no specific symptoms or clinical signs of parotid tuberculosis, and such an infection most commonly presents as a slow growing painless parotid mass. because of its rarity, tuberculosis of parotid gland is often mistaken for a malignant growth, and it most commonly gets diagnosed after superficial protidectomy. complete cur ... | 2017 | 28709482 |
| diagnosis of tuberculous meningitis: current scenario from a tertiary neurocare centre in india. | tuberculous meningitis (tbm) is a condition that is caused by mycobacterium tuberculosis complex and is difficult to diagnose due to the nonspecificity of the presentations. the study analyzed the different modes of diagnosis available in a developing country set up over a period of five years to understand the diagnostic values of the current conventional and automated methods of diagnosis of tbm among the patients suspected with chronic meningitis. | 2017 | 28709486 |
| endobronchial ultrasound experience in a high tuberculosis prevalence setting. | most of the published endobronchial ultrasound-guided transbronchial needle aspiration (ebus-tbna) data are from the western countries, establishing the role of ebus-tbna in the diagnosis and staging of lung cancer. the etiology of mediastinal lymphadenopathy may be different in an ethnic group with a high prevalence of tuberculosis (tb). | 2017 | 28709488 |
| band pattern analysis of mutations in rifampicin resistance strain of mycobacterium tuberculosis by line probe assay in patients from delhi, india. | the genotype mtbdrplus, a commercial line probe assay (lpa) kit from hain lifescience, germany, is endorsed by india's rntcp program for diagnosis of drtb cases among smear-positive sputum samples. although the lpa has been studied in several laboratories, there is a wide variation in existing m. tuberculosis strains across the globe, and false results can occur due to the presence of unique genetic mutations in different settings. | 2017 | 28709491 |
| multiple abdominal abscesses - a not so common presentation of ntm. | non-tuberculous mycobacteria/mycobacterium other than tuberculosis (mott) are ubiquitous organisms. they are acid fast bacilli often giving trouble to the physician to distinguish it from mycobacterium tuberculosis. these organisms are a menace for the treating physician as when to treat and when not to treat. they are often difficult to diagnose and may present in a variety of forms with propensity to cause number of infections of different body parts and organs. they are more common in immunoc ... | 2017 | 28709494 |
| drug-resistant tuberculosis coexisting with invasive candidiasis in an immunocompetent 30-year-old woman: a case report. | invasive candidiasis coexisting with drug-resistant mycobacterium tuberculosis (dr-tb) in the immunocompetent patient is a rare entity. we report a 30-year-old woman, nondiabetic, who presented to us with complaints of acute onset cough, breathlessness, and fever since 20 days. on thorough investigations, she was diagnosed to be suffering from coexisting drug-resistant tuberculosis and invasive candidiasis. prompt treatment initiated at right time helped us in saving her life. the unique present ... | 2017 | 28709496 |
| bactericidal impact of ag, zno and mixed agzno colloidal nanoparticles on h37rv mycobacterium tuberculosis phagocytized by thp-1 cell lines. | the purpose of this research project was to infection of human macrophages (thp-1) cell lines by h37rv strain of mycobacterium tuberculosis (h37rvmtb) and find out the ratio/dilution of mixture silver (ag nps) and zinc oxide nanoparticles (zno nps) whose ability to eliminate phagocytized bacteria compared to rifampicin. the colloidal ag nps and zno nps were synthesized and their characteristics were evaluated. the thp-1 cell lines were infected with different concentration of h37rvmtb. next, the ... | 2017 | 28710015 |
| peritoneal tuberculosis as a cause of ascites in a patient with cirrhosis. | a 59-year-old portuguese caucasian man with a history of heavy alcohol intake and no significant medical history presented with ascites, weight loss and general malaise. the ascitic fluid analysis showed 921 cells/mm(3) with mononuclear predominance (93.6%), elevated total proteins and a slightly elevated serum-ascites albumin gradient. the abdominal ultrasound confirmed the presence of chronic liver disease with ascites, and additionally on ct there was evidence of peritoneal thickening. on rep ... | 2017 | 28710241 |
| combining molecular typing and spatial pattern analysis to identify areas of high tuberculosis transmission in a moderate-incidence county in taiwan. | in total, 303 randomly selected clinical mycobacterium tuberculosis (mtb) isolates from 303 patients (collected january to december 2012) in central taiwan were examined. the major lineages found were beijing (n = 114, 37.62%), haarlem (n = 76, 25.08%) and east african-indian (eai) (n = 42, 13.86%). notably, younger persons (≤30 years old) were 6.58 times more likely to be infected with a beijing genotype compared to older persons (>70 years) (p < 0.05). combining molecular typing methods and ge ... | 2017 | 28710410 |
| breaking transmission with vaccines: the case of tuberculosis. | members of the mycobacterium tuberculosis complex (mtbc) have evolved causing tuberculosis (tb) in different mammalian hosts. mtbc ecotypes have adapted to diverse animal species, with m. bovis being the most common cause of tb in livestock. cattle-to-human transmission of m. bovis through ingestion of raw milk was common before introduction of the pasteurization process. tb in humans is mainly caused by m. tuberculosis. this bacterium is considered a genetically clonal pathogen that has coevolv ... | 2017 | 28710848 |
| a multistage subunit vaccine effectively protects mice against primary progressive tuberculosis, latency and reactivation. | adult tuberculosis (tb) is the main cause of tb epidemic and death. the infection results mainly by endogenous reactivation of latent tb infection and secondarily transmitted by exogenous infection. there is no vaccine for adult tb. to this end, we first chose antigens from a potential antigenic reservoir. the antigens strongly recognized t cells from latent and active tb infections that responded to antigens expressed by mycobacterium tuberculosis cultured under different metabolic states. fusi ... | 2017 | 28711483 |
| extended spectrum of antibiotic susceptibility for tuberculosis, djibouti. | the horn of africa suffers from a high prevalence of tuberculosis which is reported to be partly driven by multi-drug resistant (mdr) mycobacterium tuberculosis strictu sensu strains. we conducted a prospective study to investigate m. tuberculosis complex species causing tuberculosis in djibouti; along with their in vitro susceptibility to standard anti-tuberculous antibiotics, in addition to clofazimine, minocycline, chloramphenicol and sulfadiazine. among the 118 mycobacteria isolates from 118 ... | 2017 | 28711677 |
| striking the right immunological balance prevents progression of tuberculosis. | tuberculosis (tb) caused by infection with mycobacterium tuberculosis (mtb) is a major burden for human health worldwide. current standard treatments for tb require prolonged administration of antimycobacterial drugs leading to exaggerated inflammation and tissue damage. this can result in the reactivation of latent tb culminating in tb progression. thus, there is an unmet need to develop therapies that would shorten the duration of anti-tb treatment and to induce optimal protective immune respo ... | 2017 | 28711989 |
| synthesis and biological evaluation of novel 1,2,3-triazole derivatives as anti-tubercular agents. | a library of seventeen novel 1,2,3-triazole derivatives were efficiently synthesized in excellent yields by the popular 'click chemistry' approach and evaluated in vitro for their anti-tubercular activity against mycobacterium tuberculosis h37ra (atcc 25177 strain). among the series, six compounds exhibited significant activity with minimum inhibitory concentration (mic) values ranging from 3.12 to 0.78μg/ml and along with no significant cytotoxicity against mbmdmqs (mouse bone marrow derived ma ... | 2017 | 28712709 |
| structural insight into the activation of pkni kinase from m. tuberculosis via dimerization of the extracellular sensor domain. | protein kinases play central roles in the survival of mycobacterium tuberculosis within host. here we report the individual high-resolution crystal structures of the sensor domain (in both monomer and dimer forms) and the kinase domain of pkni, a transmembrane protein member of the serine/threonine protein kinases (stpks) family. pkni is the first stpk identified whose sensor domain exists in a monomer-dimer equilibrium. inspection of the two structures of the sensor domain (pkni_sd) revealed co ... | 2017 | 28712808 |
| nanoparticle-formulated curcumin prevents posttherapeutic disease reactivation and reinfection with mycobacterium tuberculosis following isoniazid therapy. | curcumin, the bioactive component of turmeric also known as "indian yellow gold," exhibits therapeutic efficacy against several chronic inflammatory and infectious diseases. even though considered as a wonder drug pertaining to a myriad of reported benefits, the translational potential of curcumin is limited by its low systemic bioavailability due to its poor intestinal absorption, rapid metabolism, and rapid systemic elimination. therefore, the translational potential of this compound is specif ... | 2017 | 28713372 |
| first insight into a nationwide genotypic diversity of mycobacterium tuberculosis among previously treated pulmonary tuberculosis cases in benin, west africa. | molecular studies on tuberculosis (tb) are rare in low-resource countries like benin, where data on molecular study on previously treated tb cases is unavailable. | 2017 | 28713434 |
| modulation of trehalose dimycolate and immune system by rv0774c protein enhanced the intracellular survival of mycobacterium smegmatis in human macrophages cell line. | mycobacterium tuberculosis rv0774c protein was reported previously to express under stress conditions. therefore, rv0774c gene was cloned and expressed in mycobacterium smegmatis, a surrogate host, to determine its role in bacterial persistence and immune modulation in natural environment. the bacterial colonies expressing rv0774c (ms_rv0774c) were larger, smoother, more moist, and flatter than the control ones (ms_ve). enhanced survival of ms_rv0774c after treatment with streptomycin was observ ... | 2017 | 28713776 |
| generation of monoclonal antibodies against ag85a antigen of mycobacterium tuberculosis and application in a competitive elisa for serodiagnosis of bovine tuberculosis. | the ag85 complex functions as the main secretory protein of mycobacterium tuberculosis (m. tuberculosis) and bcg. this complex is composed of the proteins, ag85a, ag85b, and ag85c, with ag85a thought to play the largest role within the complex. however, the lack of commercially available monoclonal antibodies (mabs) against ag85a still hinders the biological and applicative research on this protein. in this study, we developed and identified anti-ag85a mabs, and five hybridoma cells were establi ... | 2017 | 28713817 |
| role of interferons in the development of diagnostics, vaccines, and therapy for tuberculosis. | tuberculosis (tb) is an airborne infection caused by mycobacterium tuberculosis (mtb). about one-third of the world's population is latently infected with tb and 5-15% of them will develop active tb in their lifetime. it is estimated that each case of active tb may cause 10-20 new infections. host immune response to mtb is influenced by interferon- (ifn-) signaling pathways, particularly by type i and type ii interferons (ifns). the latter that consists of ifn-γ has been associated with the prom ... | 2017 | 28713838 |