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the role of free amino acids in semen of rainbow trout oncorhynchus mykiss and carp cyprinus carpio.the present study investigated (1) the free amino acid (faa) composition in semen of rainbow trout oncorhynchus mykiss and carp cyprinus carpio, (2) enzyme systems involved in amino acid metabolism and (3) the effect of amino acids on sperm viability under in vitro storage conditions. in the seminal plasma of o. mykiss, the main faas were arginine, glutamic acid, isoleucine, leucine, methionine and proline, in spermatozoa cysteine, arginine and methionine. in the seminal plasma of c. carpio, the ...200920738581
a one-year investigation of the relationship between serum gh levels and the growth of f(4) transgenic and non-transgenic common carp cyprinus carpio.it has been demonstrated that growth hormone (gh) transgenic fish often posses a trait for fast growth. here, we investigated the growth of f(4)'all-fish' gh transgenic carp cyprinus carpio and their serum gh levels for a year. the results showed that f(4) all-fish gh transgenic carp were significantly larger in body mass (c. two-fold, p < 0.001) and body length (c. 1.3 fold, p < 0.001), compared with the non-transgenic group. the discrepancy of serum gh levels between the transgenic carp group ...200920738600
behavioural and morphological differences between feral and domesticated strains of common carp cyprinus carpio.morphological and behavioural traits of a feral strain of the common carp cyprinus carpio from lake biwa in japan were compared with those of two domesticated strains reared in japan (one commercial strain and one ornamental koi). to compare genetically inherited traits, all fish were reared from eggs under similar environmental conditions. using these fish, the following five traits were compared among the three strains: body shape, consumption rate of two types of free-swimming shrimp, medaka ...200920738609
population subdivision in siamese mud carp henicorhynchus siamensis in the mekong river basin: implications for management.a molecular approach was employed to investigate stock structure in siamese mud carp henicorhynchus siamensis populations collected from 14 sites across mainland south-east asia, with the major focus being the lower mekong river basin. spatial analysis of a mitochondrial dna fragment (atpase 6 and 8) identified four stocks in the mekong river basin that were all significantly differentiated from a population in the nearby khlong river, thailand. in the mekong river basin, populations in northern ...200920738620
elevated ability to compete for limited food resources by 'all-fish' growth hormone transgenic common carp cyprinus carpio.food consumption, number of movements and feeding hierarchy of juvenile transgenic common carp cyprinus carpio and their size-matched non-transgenic conspecifics were measured under conditions of limited food supply. transgenic fish exhibited 73.3% more movements as well as a higher feeding order, and consumed 1.86 times as many food pellets as their non-transgenic counterparts. after the 10 day experiment, transgenic c. carpio had still not realized their higher growth potential, which may be p ...200920738625
development and characterization of microsatellite markers to study population genetic diversity of przewalski's naked carp gymnocypris przewalskii in china.two populations of przewalski's naked carp gymnocypris przewalskii, 30 individuals per population, were screened for 10 microsatellite loci. moderate allele variation was found in these loci with two to eight alleles per locus. the expected and observed heterozygosity ranged from 0.019 to 0.805 and from 0.160 to 0.575, respectively.200920738692
transmission dynamics of an emerging infectious disease in wildlife through host reproductive cycles.emerging infectious diseases are major threats to wildlife populations. to enhance our understanding of the dynamics of these diseases, we investigated how host reproductive behavior and seasonal temperature variation drive transmission of infections among wild hosts, using the model system of cyprinid herpesvirus 3 (cyhv-3) disease in common carp. our main findings were as follows: (1) a seroprevalence survey showed that cyhv-3 infection occurred mostly in adult hosts, (2) a quantitative assay ...201120740025
inclusion of atlantic salmon in the chinese diet reduces cardiovascular disease risk markers in dyslipidemic adult men.although the beneficial effects of n-3 fatty acids on several physiologic functions have been widely reported, information about the effects of oily fish in the asian diet on cardiovascular disease (cvd) risk is diminutive. we hypothesize that daily inclusion of oily fish for 8 weeks in the chinese diet will elevate serum eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) levels and reduce cvd risk markers in dyslipidemic adult men, comparable with the effects generally observed by inclu ...201020797476
[effect of chronic anosmia on morphofunctional parameters of the gustatory system primary centers of the carp cyprinus carpio].morphofunctional state of neurons in primary centers of olfactory, external and intraoral gustatory systems in carp yearlings was studied at different time after bilateral anosming, with use of methods of morphometrical, biochemical, and histochemical analyses. the olfactory deafferentation produces centripetal translocation of olfactory bulbs and development of their degenerative changes. chronic elimination of olfactory reception in carps leads to activation of neurons in the facial lobe of me ...201020799610
application of enzymatic probe sonication extraction for the determination of selected veterinary antibiotics and their main metabolites in fish and mussel samples.a new method based on enzymatic probe sonication extraction prior to high-performance liquid chromatography (hplc) has been developed for the determination of 11 antibiotics (drugs) and the main metabolites of five of them in fish tissue and mussel samples. the analytes belong to four different classes of antibiotics (sulfonamides, tetracyclines, penicillins and amphenicols). the analysed compounds were sulfadiazine (sdi) and n(4)-acetylsulfadiazine (ndi) metabolite, sulfamethazine (smz) and n(4 ...201020800727
integrated biological and chemical analysis of organochlorine compound pollution and of its biological effects in a riverine system downstream the discharge point.pollution in riverine systems, along with its biological effects, may propagate downstream even at considerable distances. we analyzed the organochlorine compound (oc) pollution in a section of the low ebro river (northeast spain) downstream a long-operating chlor-alkali plant. maximal levels of ocs and of their associated dioxin-like biological activity occurred in residue samples from the plant, and persisted in river sediments some 40km downstream (xerta site). biological analysis at multiple ...201020800876
fetuin-a and cystatin c are endogenous inhibitors of human meprin metalloproteases.meprin α and β, zinc metalloproteinases, play significant roles in inflammation, including inflammatory bowel disease (ibd), possibly by activating cytokines, like interleukin 1β, interleukin 18, or tumor growth factor α. although a number of potential activators for meprins are known, no endogenous inhibitors have been identified. in this work, we analyzed the inhibitory potential of human plasma and identified bovine fetuin-a as an endogenous meprin inhibitor with a k(i) (inhibition constant) ...201020806899
cell cycle and apoptosis regulatory protein (carp)-1 is a novel, adriamycin-inducible, diffuse large b-cell lymphoma (dlbl) growth suppressor.diffuse large b-cell lymphoma (dlcl) accounts for 30-40% of adult non-hodgkin's lymphoma (nhl). current anti-nhl therapies often target cellular growth suppression pathways and include r-chop (cyclophosphamide, adriamycin, vincristine, and prednisone plus monoclonal anti-cd20 antibody rituximab). however, since many patients relapse, resistant cells to these therapies remain a significant problem and necessitate development of new intervention strategies. cell cycle and apoptosis regulatory prot ...201020809119
influence of a toxic microcystis aeruginosa strain on glutathione synthesis and glutathione-s-transferase activity in common carp cyprinus carpio (teleostei: cyprinidae).we evaluated the effects of aqueous extracts of the cyanobacterium-producing microcystin (mc), microcystis aeruginosa (strain rst9501), on detoxification capacity and glutathione (gsh) synthesis in liver, brain, gill, and muscle-as well as apoptotic protease (calpain) activity in liver and brain-in the common carp cyprinus carpio (teleostei: cyprinidae). experimental groups were defined as follows: (1) control (ctr); (2) carp treated with an aqueous extract from the toxic cyanobacteria m. aerugi ...201120809345
oxidative stress biomarkers in cyprinus carpio exposed to commercial herbicide bispyribac-sodium.cyprinus carpio were exposed under field conditions to 20.87 microg l(-1) of commercial herbicide bispyribac-sodium (nominee, sc), during 7, 21 and 72 days. enzymatic parameters such as catalase (cat), glutathione s-transferase (gst) and acetylcholinesterase (ache) activities, as well as thiobarbituric acid-reactive substances (tbars) and protein carbonyl contents were studied in different tissues. after 7 days of exposure, gst activity decreased. at the same period, brain ache activity increase ...201020809548
perforin-dependent cytotoxic mechanism in killing by cd8 positive t cells in ginbuna crucian carp, carassius auratus langsdorfii.t cell-mediated cytotoxicity occurs via pathways based on perforin or fas mechanisms. perforin is a protein present in the cytoplasmic granules of cd8(+) cytotoxic t lymphocytes and is secreted to form pores on target cell membranes. in fish, although the involvement of perforin in cytotoxicity have been suggested for several species, perforin-mediated cytotoxicity of cd8α(+) lymphocyte in conjunction with expression of the perforin gene has not been reported. in order to investigate the killing ...201120813128
toxicity of organic pollutants to seven aquatic organisms: effect of polarity and ionization.the toxicity of organic chemicals to vibrio fischeri, river bacteria, algae, daphnia magna and fishes were analysed. the results showed that the toxicity of chemicals to narcotics was dependent on hydrophobicity. a single model for both polar and non-polar narcotics was developed by inclusion of a polarity descriptor as well as the hydrophobic parameter. the highly hydrophobic polar narcotics could be treated as non-polar narcotics because their polar functional group(s) make(s) a relatively sma ...201020818578
immunomodulatory effect of aegle marmelos leaf extract on freshwater fish cyprinus carpio infected by bacterial pathogen aeromonas hydrophila.aquatic organisms (especially fish) require potent defense mechanisms to protect themselves against pathogen invasion and disease formation. the use of immunostimulants in fish culture can prevent the diseases through augmentation of both specific and non-specific immunity.201020818950
carbonic anhydrase related protein viii and its role in neurodegeneration and cancer.mammalian carbonic anhydrase (α-ca) gene family comprises sixteen isoforms, thirteen of which are active isozymes and three isoforms lack classical ca activity of reversible hydration of co(2) due to absence of one or more histidine residues required for ca catalytic activity. the inactive isoforms are known as carbonic anhydrase related proteins (carps) viii, x and xi. among these three, carp viii was reported first in 1990 from a mouse brain cdna library and is well studied structurally as wel ...201020819067
the influence of diet on the assimilation efficiency of 47 polychlorinated biphenyl congeners in japanese koi (cyprinus carpio).the influence of diet on polychlorinated biphenyl (pcb) assimilation was investigated by measuring dietary assimilation efficiencies (aes) for 47 pcb congeners in juvenile koi (cyprinus carpio) fed five experimental diets. two of the diets were naturally contaminated and were obtained by collecting mayflies from lake erie (on, canada) and emerald shiners from the detroit river (mi, usa). the remaining diets consisted of commercial fish pellets (lipid contents from 6.7 to 24%) that were contamina ...201020821460
effect of nitrite on early-life stages of common carp (cyprinus carpio l.).a one-month chronic exposure of common carp larvae and embryos to nitrite revealed significant (p < 0.01) differences in total accumulated mortality in fish exposed to 33, 67, and 330 mg/l no(2)(-) compared with controls. at the highest concentration, all fish died within 8 d of exposure. on the basis of accumulated mortality in the experimental groups, lethal concentrations of nitrite were estimated at 29 d lc50 = 88 mg/l no(2)(-); lowest-observed-effect concentration (loec) = 28 mg/l no(2)(-); ...201020821475
microcystin-lr modulates selected immune parameters and induces necrosis/apoptosis of carp leucocytes.microcystins (mcs) are potent hepatotoxins acting by the inhibition of protein phosphatase 1 and 2a, and may promote liver tumors. moreover, studies also suggest they are nephrotoxic. the aim of the present study was to assess possible in vitro effects of microcystin-lr (which contains the amino acids leucine and arginine, the most widely studied and distributed variant of all microcystins) on the selected immune functions of the cells isolated from the head kidney of carp. in the experiments, p ...201020821480
hemolysin etha in edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system esra-esrb and nucleoid protein hhaet.edwardsiella tarda is a gram-negative pathogen for hemorrhagic septicemia in fish. recently, two-component system (tcs) esra-esrb in e. tarda has been found to play key roles in regulating type iii secretion system (ttss) and type vi secretion system (t6ss). in this study, a markedly attenuated δesrb mutant was investigated to exhibit enhanced cell-invasion capability, as well as the increased cytotoxicity of its extracellular products (ecps). compared with the parental strain, the δesrb mutant ...201020832475
variability of responses in the crucian carp carassius carassius from two ukrainian ponds determined by multi-marker approach.the aim of this study was to evaluate the health status of the comparatively tolerant fish carassius carassius over three seasons in an area characterized by spontaneous human activities. sites near the springs of a river (site z) and downstream of a river (site b) in western ukraine were selected. according to the centroid grouping analysis, the biochemical and morphological indices allowed the fish to be distinguished according to season more than to site. the level of nuclear abnormalities wa ...201020832861
ultrastructural aspects of spermatogenesis, testes, and vas deferens in the parthenogenetic tapeworm atractolytocestus huronensis anthony, 1958 (cestoda: caryophyllidea), a carp parasite from slovakia.spermatogenesis, testes, and vas deferens in the parthenogenetic monozoic tapeworm atractolytocestus huronensis anthony, 1958 (cestoda: caryophyllidea) from slovakia, parasitizing the carp cyprinus carpio l., have been investigated by means of transmission electron microscopy for the first time. the present results show that helminths with parthenogenetic and normal reproduction may share some common spermatology features, e.g., dense cytoplasm of the peripherally localized spermatogonia or a ro ...201120838810
protective effect of zinc on related parameters to bone metabolism in common carp fish (cyprinus carpio l.) intoxified with cadmium.the short term effects of waterborne cadmium (cd(+2)) on the levels of serum parameters related to bone metabolism including calcium (ca), inorganic phosphorus (p(i)) and alkaline phosphatase (alp) in common carp fish (cyprinus carpio l.) were studied. fish were treated with varying concentrations of cd(+2) (0.22, 1.1 and 2.2 mg l(-1)) daily for 14 days. the results obtained show that serum p(i) and alp concentrations were elevated by increasing cd(+2) concentration in water containing fish wher ...201120839051
sequence variation and secondary structure analysis of the first ribosomal internal transcribed spacer (its-1) between cyprinus carpio carpio and c. carpio haematopterus. 201120848177
at least two expressed genes for transcription factors pitx2 and rpx are present in common carp and are upregulated during winter acclimatization.the mechanisms of seasonal acclimatization in eurythermal fish such as common carp are not fully understood. here, we concentrate on the regulation of pituitary factors, as this organ was shown to be highly affected by seasonal changes. we cloned and sequenced two different cdnas for each of the transcription factors pitx2 and rpx, known to play a role in pituitary development. we show that these genes are conserved throughout evolution, to different degrees depending on the specific domain cons ...201020850444
the lim protein zyxin binds carp-1 and promotes apoptosis.zyxin is a dual-function lim domain protein that regulates actin dynamics in response to mechanical stress and shuttles between focal adhesions and the cell nucleus. here we show that zyxin contributes to uv-induced apoptosis. exposure of wild-type fibroblasts to uv-c irradiation results in apoptotic cell death, whereas cells harboring a homozygous disruption of the zyxin gene display a statistically significant survival advantage. to gain insight into the molecular mechanism by which zyxin prom ...201020852740
a new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-κb pathway in skeletal muscle.a multiprotein complex encompassing a transcription regulator, cardiac ankyrin repeat protein (carp), and the calpain 3 protease was identified in the n2a elastic region of the giant sarcomeric protein titin. the present study aimed to investigate the function(s) of this complex in the skeletal muscle. we demonstrate that carp subcellular localization is controlled by the activity of calpain 3: the higher the calpain 3, the more important the sarcomeric retention of carp. this regulation would o ...201020860623
the effect of extenders, cryoprotectants and cryopreservation methods on common carp (cyprinus carpio) sperm.the objective of this study was to determine the effects of various extenders, cryoprotectants and cryopreservation methods on post-thaw sperm motility and duration of sperm motility in common carp (cyprinus carpio). we focused on freezing of common carp sperm utilizing a practical and inexpensive protocol for aquaculture. sperm were diluted 1:1 in one of six extenders (common carp sperm extenders; ccse 1-ccse 6) containing three types of cryoprotectants (dimethyl sulfoxide; dmso, methanol; met ...201020863635
cxcl8 chemokines in teleost fish: two lineages with distinct expression profiles during early phases of inflammation.during the inflammatory process, chemokine cxcl8 plays a pivotal role in recruitment of human neutrophilic granulocytes. a diversity of sequences similar to cxcl8 was reported in fish, but their evolutionary relationships and functional homology with their human homolog remain unclear.201020865040
hepatoprotective and antioxidant effects of glycyrrhiza glabra extract against carbon tetrachloride (ccl(4))-induced hepatocyte damage in common carp (cyprinus carpio).the present study is aiming at evaluating the hepatoprotective and antioxidant effects of glycyrrhiza glabra extract (2.5, 5 and 10 μg/ml) on the carbon tetrachloride (ccl(4))-induced carp hepatocyte damage in vitro. glycyrrhiza glabra extract was added to the carp primary hepatocytes before (pre-treatment), after (post-treatment) and both before and after (pre- and post-treatment) the incubation of the hepatocytes with ccl(4). ccl(4) at 8 mm in the culture medium produced significantly elevated ...201120865324
effects of gas supersaturation on lethality and avoidance responses in juvenile rock carp (procypris rabaudi tchang).laboratory experiments were conducted to determine the effects of total dissolved gas (tdg) supersaturation on acute lethality and avoidance responses in juvenile rock carp (procypris rabaudi tchang). the juvenile rock carp were exposed to water with different levels of supersaturation (105%, 115%, 120%, 125%, 130%, 135%, 140%, and 145%) and depth of 0.20 m at 25 °c for 60 h. median lethal time (lt(50)) was used to assess the lethal responses corresponding to different levels of gas supersaturat ...201020872989
clonal growth of carp (cyprinus carpio) t cells in vitro.carp kidney leukocytes co-cultured with a supporting cell layer resulted in the rapid proliferation of various types of leukocytes including immature leukocytes. expressions of marker genes for multiple blood cell lineages were observed in the primary culture. however, after several passages, the proliferating cells expressed only t cell and macrophage marker genes. further rt-pcr analysis revealed that the proliferating cells expressed tcr constant regions (cα, cβ, cγ, cδ), cd3γ/δ and cd4 (cd4l ...201120875447
simultaneous determination of hexabromocyclododecanes and tris (2,3-dibromopropyl) isocyanurate using lc-apci-ms/ms.hexabromocyclododecanes (hbcds) and tris (2,3-dibromopropyl) isocyanurate (tbc) are both brominated flame retardants, and have been used throughout the world with subsequent concern for potential environment pollution. here we describe the development of a method for the simultaneous determination of tbc and hbcd diastereoisomers in environmental matrices by high performance liquid chromatography tandem mass spectrometry (hplc-ms/ms). the method recovery ranged from 81% to 93% and limits of dete ...201020875598
cdna cloning, pituitary location, and extra-pituitary expression of pro-opiomelanocortin gene in rare minnow (gobiocypris rarus).a cdna encoding pro-opiomelanocortin (pomc) gene was cloned from the pituitary gland of the rare minnow (gobiocypris rarus), a small freshwater fish endemic to china. this was achieved by reverse transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). data showed that the predicted rare minnow pomc (rmpomc) cdna consisted of 846bps coding for the following sequences, flanked by proteolytic cleavage sites: signal peptide (sp, met(1)-ala(28)), n-terminal pepti ...201120878469
a novel (18)o inverse labeling-based workflow for accurate bottom-up mass spectrometry quantification of proteins separated by gel electrophoresis.in the present work we report on a novel and fast protocol for accurate bottom-up protein quantification that overcomes the drawbacks of in-gel digestion and maldi analysis, while maintaining their benefits. it relies on the following steps: (i) gel electrophoresis separation of proteins, (ii) fast in-gel protein digestion with trypsin, (iii) (18)o-labeling through the decoupled method, (iv) quantification through selected peptides previously chosen using the (18)o inverse labeling approach and ...201020882554
¹h nmr-based metabolomics approach to assess toxicity of bunker a heavy oil to freshwater carp, cyprinus carpio.using a ¹h nmr metabolomics approach, the effects of dietary exposure of bunker a heavy oil (0.01, 0.1, 1, and 5% in diet) on freshwater carp, cyprinus carpio, were examined. statistical analysis by pca score plots showed that the amount of metabolites in exposure groups 0.1, 1, and 5% differed from those in the control group. although no discernible effects on metabolites were noted in the 0.1% exposure group as well as in the lowest concentration (0.01%) group, several metabolites such as amin ...201220882592
genotoxic and cytotoxic effects induced by aluminum in the lymphocytes of the common carp (cyprinus carpio).few studies have been made in regard to the effect of aluminum on the molecular and cellular structure and function of aquatic organisms; therefore, in the present report we determined the genotoxic and cytotoxic effects induced by the metal on the lymphocytes of carp (cyprinus carpio). three groups of fish were exposed to 0.05, 120, and 239 mg/l of aluminum (al), respectively, by using al₂ (so₄)₃·7h₂o, and another group was included as control. the cells obtained were studied with the comet ass ...201120883821
trypanosomiasis-induced th17-like immune responses in carp.in mammalian vertebrates, the cytokine interleukin (il)-12 consists of a heterodimer between p35 and p40 subunits whereas interleukin-23 is formed by a heterodimer between p19 and p40 subunits. during an immune response, the balance between il-12 and il-23 can depend on the nature of the pathogen associated molecular pattern (pamp) recognized by, for example tlr2, leading to a preferential production of il-23. il-23 production promotes a th17-mediated immune response characterized by the product ...201020885956
genetic linkage mapping and analysis of muscle fiber-related qtls in common carp (cyprinus carpio l.).a genetic linkage map of common carp (cyprinus carpio l.) was constructed using type i and type ii microsatellite markers and a pseudo-testcross mapping strategy. the microsatellite markers were isolated from microsatellite-enriched genomic libraries and tested for their segregation in a full-sib mapping panel containing 92 individuals. a total of 161 microsatellite loci were mapped into 54 linkage groups. the total lengths of the female, male and consensus maps were 2,000, 946, and 1,852 cm, wi ...201120886255
exposure to tebuconazol in rice field and laboratory conditions induces oxidative stress in carp (cyprinus carpio).pesticides can have an effect on the biochemical and physiological functions of living organisms. the changes seen in fish and their response to pesticides can be used as an example for vertebrate toxicity. in this study, carp fish (cyprinus carpio) were exposed to different concentrations of tebuconazol fungicide, by rice field (31.95 μg/l) and laboratory (33.47 and 36.23 μg/l) conditional testing, during a 7 day period. parameters such thiobarbituric acid-reactive substance levels (tbars), pro ...201120888428
analysis of the conformations corresponding to hexapeptide and large sequences characterized by continuous single amino acid repeats in proteins.the analysis of conformations corresponding to continuous amino acid repeat peptides (carps) comprising six or more residues in proteins of known three-dimensional structure revealed that alanine, glycine, glutamic acid, proline, valine, histidine, aspartic acid, glutamine and lysine were associated as repeating amino acid residues. alanine, glycine and histidine carps were most common, although the histidine hexapeptide and large carps mainly correspond to affinity tags and are not part of the ...201020937037
haematopoiesis in the head kidney of common carp (cyprinus carpio l.): a morphological study.the morphogenesis of head kidney haematopoietic cells in common carp has been shown to be very similar to that of higher vertebrates. the erythropoietic series consisted of the erythroblasts (basophilic, polychromatic and orthochromic), young erythrocyte and erythrocyte. the morphological changes that occur during maturation process are reduction in size and further increase at mature stage, gradual chromatin condensation and the increase in haemoglobin content. maturation stages of neutrophils ...201120960053
changes of globin expression in the japanese medaka (oryzias latipes) in response to acute and chronic hypoxia.fishes live in an aquatic environment with low or temporally changing o(2) availability. variations in o(2) levels require many anatomical, behavioral, physiological, and biochemical adaptations that ensure the uptake of an adequate amount of o(2). some fish species are comparatively well adapted to tolerate low o(2) partial pressure (hypoxia). the japanese ricefish medaka (oryzias latipes) is an important model organism for biomedical research that shows remarkable tolerance towards hypoxia. we ...201120963423
mass mortality associated with koi herpesvirus in wild common carp in canada.koi herpesvirus (khv) was identified as being associated with more than one mortality event affecting common carp in canada. the first was an extensive mortality event that occurred in 2007 in the kawartha lakes region, ontario, affecting lakes scugog and pigeon. fish had branchial necrosis and hepatic vasculitis with an equivocal interstitial nephritis. several fish also had branchial columnaris. subsequent mortality events occurred in 2008 in additional bodies of water in south-central ontario ...201020966274
the tissue factor pathway inhibitor 1 of sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection.tissue factor pathway inhibitor 1 (tfpi-1) is a kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. in teleosts, tfpi-1-like sequences have been found to exist in two species (danio rerio and cyprinus carpio); however, the potential function of fish tfpi-1 has not been investigated. in this study, we identified and analyzed a tfpi-1 homologue, sotfpi-1, from red drum (sciaenops ocellatus). the deduced amino acid sequence of sotfpi-1 is 284 re ...201020970444
mutagenic impact on fish of runoff events in agricultural areas in south-west france.when heavy rainfall follows herbicide application, the intense surface runoff causes stream water contamination. aquatic organisms are then briefly exposed to a complex mixture of contaminants. the aim of the present study is to investigate the genotoxic impact of such events on fish. a model fish, the crucian carp (carassius carassius) was exposed in controlled conditions, for 4 days, to water sampled daily in the save river (france). the watershed of this stream is representative of agricultur ...201120970859
prevalence and sequence comparison of phyllodistomum folium from zebra mussel and from freshwater fish in the ebro river.we utilised dna analysis to detect the presence of the digenean phyllodistomum folium in three cyprinid species, scardinius erythrophthalmus, cyprinus carpio and rutilus rutilus. dna sequencing of the region containing the genes its1-5.8s-its2 revealed 100% sequence identity between dna from the sporocysts found in zebra mussels and dna from adults located in the urinary system of 29 cyprinid fish. a second genetically different (variation=1.6%) sequence was observed in two samples from r. rutil ...201020971214
rates and patterns of microsatellite mutations in common carp (cyprinus carpio l.).during genotyping 150 microsatellites in a f1 family of common carp, six mutations were found at five microsatellite loci. the overall mutation rate of common carp microsatellites was 2.53 x 10(-4) per locus per generation. at five loci, mutations increased the length of alleles by at least one repeat unit, suggesting mutations at microsatellite loci in common carp do not follow strict stepwise mutation model. the data on mutation rates and patterns can facilitate population genetics studies, an ...201020979260
nutritive value of fish from michigan waters; nicotinic acid of lake herring, carp, common suckers, and burbot. 201621025062
derivation and characterization of embryonic stem-like cells of indian major carp catla catla.embryonic stem (es)-like cells were derived from mid-blastula stage embryos of a freshwater fish, catla catla catla, under feeder-free condition and designated as cces cells. the conditioned media was optimized with 10% foetal bovine serum (fbs), fish embryo extract (fee) having 100 µg ml(-1) protein concentration, 15 ng ml(-1) basic fibroblast growth factor (bfgf) and basic media containing leibovitz-15, dmem with 4·5 g l(-1) glucose and ham's f12 (ldf) in 2:1:1 ratio using a primary culture of ...201021039493
population genetics of invasive common carp cyprinus carpio l. in coastal drainages in eastern australia.the common carp cyprinus carpio introduced in two drainages in eastern australia are largely descended from european common carp, and in a third drainage they descend largely from east asian common carp. the partial genetic differentiation among the species in those drainages is consistent with their origins.201021039496
identification of calcium-binding proteins in fish seminal plasma.calcium ions play an important role in the activation of fish sperm movement. the mechanism of their binding in semen is still unknown. the goal of this study was the development of a method for identifying calcium-binding proteins in fish seminal plasma. two methods of calcium-binding proteins detection were tested with the use of quin2 and stains-all dyes. the first method was useful for the identification of calcium-binding proteins of fish seminal plasma. it consisted of proteins separation ...201021042848
variations in physico-chemical and microbiological characteristics of water during breeding of cyprinus carpio in a closed hatchery system.physico-chemical and microbial characteristics of culture water were examined during the induced breeding of cyprinus carpio in a controlled environmental system. water temperature, dissolved oxygen, nitrate, phosphate, ph, ammonia nitrogen, total bacterial count, hardness, salinity, carbonate and bicarbonate were estimated before and after spawning and hatching. average alteration in water ph before and after spawning was 7.91-7.57 and 7.86-7.58 respectively. total hardness, carbonate and bicar ...201021047001
expression of immune-related genes in rohu labeo rohita (hamilton) by experimental freshwater lice argulus siamensis (wilson) infection.the crustacean ectoparasite, argulus poses one of the major threats to carp culture due to absence of any suitable control measure. the study was undertaken to determine the expression of immune-related genes in three major immunocompetent organs viz., kidney, skin and liver of rohu (labeo rohita) during experimental freshwater lice argulus siamensis infection. results showed that the expression of tlr 22-like, lysozyme g and β2-microglobulin genes in kidney was significantly (p ≤ 0.05) down-reg ...201021051150
native fish diversity alters the effects of an invasive species on food webs.aquatic communities have been altered by invasive species, with impacts on native biodiversity and ecosystem function. at the same time, native biodiversity may mitigate the effects of an invader. common carp (cyprinus carpio) is a ubiquitous, invasive fish species that strongly influences community and ecosystem processes. we used common carp to test whether the potential effects of an invasive species are altered across a range of species diversity in native communities. in mesocosms, treatmen ...201021058556
biologic responses of bacteria communities living at the mucus secretion of common carp (cyprinus carpio) after exposure to the carbon nanomaterial fullerene (c(60)).bacteria communities living in mucus secretions of common carp cyprinus carpio (cyprinidae) were exposed to the organic nanomaterial fullerene (c(60)) to evaluate its potential bactericidal effects. end points analyzed were viability, growth, reactive oxygen species (ros) concentration, and total antioxidant competence against peroxyl radicals. viability was not affected (p > 0.05), whereas growth was arrested (p < 0.05) after 3 hours of exposure to the three concentration of c(60) assayed (0.1, ...201021072630
hepatoprotective and antioxidant effects of hibiscus sabdariffa extract against carbon tetrachloride-induced hepatocyte damage in cyprinus carpio.the present study aims to evaluate the hepatoprotective and antioxidant effects of hibiscus sabdariffa extract on the carbon tetrachloride (ccl(4))-induced hepatocyte damage in fish and provide evidence as to whether it can be potentially used as a medicine for liver diseases in aquaculture. h. sabdariffa extract (100, 200, and 400 μg/ml) was added to the carp primary hepatocyte culture before (pre-treatment), after (post-treatment), and both before and after (pre- and post-treatment) the incuba ...201121082285
characterization of fish irf3 as an ifn-inducible protein reveals evolving regulation of ifn response in vertebrates.in mammals, ifn regulatory factor (irf) 3 is a critical player in modulating transcription of type i ifn and ifn-stimulated genes (isgs). in this study, we describe the roles of crucian carp (carassius auratus l.) irf3 in activating fish ifn and isgs. fish irf3 exhibits a large sequence divergence from mammalian orthologs. whereas mammalian irf3 is constitutively expressed, fish irf3 protein is significantly upregulated by ifn, poly-ic, and other stimuli known as ifn inducers in mammals. the ifn ...201021084665
molecular cloning, characterization and expression analysis of tlr9, myd88 and traf6 genes in common carp (cyprinus carpio).induction of innate immune pathways is critical for early host defense, but there is limited understanding of how teleost fishes recognize pathogen molecules and activate these pathways. in mammals, cells of the innate immune system detect pathogenic molecular structures using pattern recognition receptors (prrs). tlr9 functions as a prr that recognizes cpg motifs in bacterial and viral dna and requires adaptor molecules myd88 and traf6 for signal transduction. here we report full-length cdna is ...201121087670
construction and characterization of the bac library for common carp cyprinus carpio l. and establishment of microsynteny with zebrafish danio rerio.a bacterial artificial chromosome (bac) library of common carp cyprinus carpio l. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. the library, containing a total of 92,160 bac clones with an average insert size of 141 kb, was constructed into the restriction site of hind iii on bac vector copycontrol pcc1bac, covering 7.7 x haploid genome equivalents. three dimension pools and superpools of the bac library were established and 23 p ...201121088980
enlarged gold-tipped silicon microprobe arrays and signal compensation for multi-site electroretinogram recordings in the isolated carp retina.in order to record multi-site electroretinogram (erg) responses in isolated carp retinae, we utilized three-dimensional (3d), extracellular, 3.5-μm-diameter silicon (si) probe arrays fabricated by the selective vapor-liquid-solid (vls) growth method. neural recordings with the si microprobe exhibit low signal-to-noise (s/n) ratios of recorded responses due to the high-electrical-impedance characteristics of the small recording area at the probe tip. to increase the s/n ratio, we designed and fab ...201121093247
hematological, biochemical and ionoregulatory responses of indian major carp catla catla during chronic sublethal exposure to inorganic arsenic.in the present study the acute toxicity of arsenic trioxide in fingerlings of catla catla an indian major carp was evaluated with renewal bioassay method. the median lethal concentration of arsenic trioxide to the fish c. catla for 96 h was found to be 20.41 ppm (with 95% confidence limits). from this a non-lethal dose of (2.041 ppm; 1/10th of lc 50 96 value) was selected and fingerlings were exposed to 35 d and hematological, biochemical and ionoregulatory responses were studied at days 7, 14, ...201121094981
toxicological responses of cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic.cyprinus carpio was exposed to imazethapyr and imazapic at laboratory and at field conditions. the laboratory experiment was carried out for 7 days and at rice field for 7, 30 and 90 days. oxidative stress parameters and antioxidant profile were studied as well as metabolic parameters. after 7 days, brain ache activity increases in laboratory and field, but in muscle, reduction was observed only in laboratory. at the same period, brain and muscle tbars and liver cat increase in the laboratory. m ...201021095003
the rapid generation of chimerical genes expanding protein diversity in zebrafish.variation of gene number among species indicates that there is a general process of new gene origination. one of the major mechanism providing raw materials for the origin of new genes is gene duplication. retroposition, as a special type of gene duplication- the rna-based duplication, has been found to play an important role in new gene evolution in mammals and plants, but little is known about the process in the teleostei genome.201021106061
molecular cloning and differential expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in hypophthalmichthys molitrix.copper/zinc superoxide dismutase (cu,zn-sod) cdna and manganese superoxide dismutase (mn-sod) cdna were first cloned from silver carp hypophthalmichthys molitrix using reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) method. the open reading frame (orf) of cu,zn-sod is 465 bp and encodes a 154 amino acids (aa) protein, whereas the orf of mn-sod is 675 bp and encodes a 224 aa protein. multiple polypeptide sequence alignment showed high identity ...201121112403
differential responses to natural and recombinant allergens in a murine model of fish allergy.aerosolized fish proteins are an important cause of allergic airway reactions in both the domestic and the occupational environment. the aim of this study was to investigate inhalant fish-induced allergy in a mouse model and compare immune responses generated by raw and heat-treated fish extracts as well as natural and recombinant forms of the major fish allergen parvalbumin. mice were sensitized with raw or cooked pilchard extract and challenged intranasally with cooked pilchard extract, purifi ...201021112638
purification and characterization of parvalbumins, the major allergens in red stingray (dasyatis akajei).fish has received increasing attention because it induces ige-mediated food allergy. parvalbumin (pv) represents the major allergen of fish, and ige cross-reactivity to pv in various teleost fish species has been shown, while little information is available about allergens in elasmobranch fish. in this study, two pv isoforms (named as pv-i and pv-ii) from red stingray (dasyatis akajei) were purified to homogeneity by a series of procedures including ammonium sulfate precipitation and column chro ...201021121608
cyprinid herpesvirus 3.the recently designated cyprinid herpesvirus 3 (cyhv-3) is an emerging agent that causes fatal disease in common and koi carp. since its emergence in the late 1990s, this highly contagious pathogen has caused severe financial losses in common and koi carp culture industries worldwide. in addition to its economic role, recent studies suggest that cyhv-3 may have a role in fundamental research. cyhv-3 has the largest genome among viruses in the order herpesvirales and serves as a model for mutagen ...201021122210
hippocampal carp over-expression solidifies consolidation of contextual fear memories.the doublecortin-like kinase (dclk) gene is involved in neuronal migration during development. through alternative splicing the dclk gene also produces a transcript called ca(2+)/calmodulin dependent protein kinase (camk)-related peptide (carp) that is expressed exclusively during adulthood in response to neuronal activity. the function of carp, however, is poorly understood. to study carp function, we have generated transgenic mice with over-expression of the carp transcript in, amongst other b ...201021130104
activity and function of rabbit muscle-specific creatine kinase at low temperature by mutation at gly268 to asn268.carp muscle-specific creatine kinase m1 isoenzyme (m1-ck) seems to have evolved to adapt to synchronized changes in body temperature and intracellular ph. when gly(268) in rabbit muscle-specific creatine kinase was substituted with asn(268) as found in carp m1-ck, the rabbit muscle-specific ck g286n mutant specific activity at ph 8.0 and 10°c was more than 2-fold higher than that in the wild-type rabbit enzyme. kinetic studies showed that k(m) values of the rabbit ck g268n mutant were similar to ...201021130895
the ontogeny of mauthner cells in the brain of labeo rohita as revealed by nadph-d and nnos immunohistochemistry.nitric oxide (no) is well demonstrated to act as a neuronal messenger in neurotransmission in vertebrate animals. we are for the first time reporting nicotinamide adenine dinucleotide phosphate-diaphorase (nadph-d) and neuronal nitric oxide synthase (nnos) in the mauthner cells (mc) of the carp, labeo rohita brain during post-embryonic development. the nadph-d and nnos-positive mc were seen intensely labeled in all young stages on either side of the ventricle in the hindbrain. in adult, these ce ...201021132510
phylogeography and conservation genetics of lake qinghai scaleless carp gymnocypris przewalskii.the objective of this study was to examine the spatial genetic relationships of the lake qinghai scaleless carp gymnocypris przewalskii within the lake qinghai system, determining whether genetic evidence supports the current taxonomy of gymnocypris przewalskii przewalskii and gymnocypris przewalskii ganzihonensis and whether gymnocypris przewalskii przewalskii are returning to their natal rivers to spawn. comparison of mitochondrial (control region) variation (42 haplotypes in 203 fish) of g. p ...201021133917
the application of hcg, cph and ovopel in successful artificial reproduction of goldfish (carassius auratus auratus) under controlled conditions.artificial reproduction of fish is one of the main goals of aquaculture production. the aim of this study is to optimize the method of goldfish reproduction under controlled conditions by comparing the effectiveness of carp pituitary homogenate (cph), ovopel and human chorionic gonadotropin (hcg), administered as a one-off dose and inducing two spawns in the same fish within a short time period. goldfish spawners were stimulated with hcg, cph and ovopel, and the results were compared to the fish ...201021134006
generation of monoclonal antibodies specific for orf68 of koi herpesvirus.outbreaks of koi herpesvirus (khv) infection in carp are still a serious problem worldwide. khv is closely related to other two cyprinid herpesviruses, pox herpesvirus (chv) and haematopoietic necrosis herpesvirus (cyhv-2) in goldfish. in this study, two major khv antigenic proteins (orf62 and orf68) were identified by immunoscreening using a khv-specific polyclonal antibody, and then monoclonal antibodies were generated for immunodiagnostic studies. after screening hybridoma cells, one mab agai ...201021134694
impact of pollutants on fish collected from different parts of shatt al-arab river: a histopathological study.in this study, a total of 120 fish specimens of common carp (cyprinus carpio), were caught at four different stations in shatt al-arab (hareer, garmat ali, al-maqal and abual-khasib). the histological changes in the gills of fish were detected microscopically. results show that the shatt al-arab is polluted by some different sources of pollutants including power stations, paper industry, oil refineries, petrochemical industry, chemical fertilizer companies and the sewage system and overfishing a ...201121140208
subcellular compartmentalization of tnf receptor-1 and cd95 signaling pathways.receptors that belong to the family of death-receptors including tnf receptor-1 (tnf-r1), cd95 (fas, apo-1) and trail receptors (trail-r1, trail r2/dr4/dr5) transduce signals resulting in entirely different biological outcomes: they promote cell death via apoptosis but are also capable of inducing anti-apoptotic signals through the transcription factor nuclear factor nf-κb or activation of the proliferative mapk/erk protein kinase cascade resulting in cell protection and tissue regeneration. rec ...201121144616
disrupting seasonality to control disease outbreaks: the case of koi herpes virus.common carp accounts for a substantial proportion of global freshwater aquaculture production. koi herpes virus (khv), a highly virulent disease affecting carp that emerged in the late 1990s, is a serious threat to this industry. after a fish is infected with khv, there is a temperature dependent delay before it becomes infectious, and a further delay before mortality. consequently, khv epidemiology is driven by seasonal changes in water temperature. also, it has been proposed that outbreaks cou ...201121145328
removal of pollutants and reduction of bio-toxicity in a full scale chemical coagulation and reverse osmosis leachate treatment system.removals of pollutants and toxic organic compounds and reduction in bio-toxicity of leachate along an operating full-scale leachate treatment system utilizing chemical coagulation, sand filtration, microfiltration (mf) and reverse osmosis (ro) membrane were evaluated. high pollutant removals were achieved mainly by coagulation and sand filtration. major toxic organic pollutants, i.e. dehp, dbp and bisphenol a were removed by 100%, 99.6% and 98.0%. acute toxicity test using water flea, nile tilap ...201121147528
visualizing a set of olfactory sensory neurons responding to a bile salt.in the present study, we exposed the olfactory epithelia of crucian carp, carassius carassius, and brown trout, salmo trutta, to dextran coupled with alexa dyes together with odorants. dye uptake was severely reduced after pre-exposure to nocodazole, an inhibitor of microtubule polymerization that impairs endocytosis, supporting the hypothesis that odour-activated olfactory receptor molecules undergo endocytosis. application of the bile acid taurolithocholate, a potent and specific odorant for f ...201121147971
conservation defines functional motifs in the squint/nodal-related 1 rna dorsal localization element.rna localization is emerging as a general principle of sub-cellular protein localization and cellular organization. however, the sequence and structural requirements in many rna localization elements remain poorly understood. whereas transcription factor-binding sites in dna can be recognized as short degenerate motifs, and consensus binding sites readily inferred, protein-binding sites in rna often contain structural features, and can be difficult to infer. we previously showed that zebrafish s ...201121149265
the individual and combined effects of deoxynivalenol and aflatoxin b(1) on primary hepatocytes of cyprinus carpio.aflatoxin b(1) (afb(1)) and deoxynivalenol (don) are important food-borne mycotoxins that have been implicated in animal and human health. in this study, individual and combinative effects of afb(1) and don were tested in primary hepatocytes of cyprinus carpio. the results indicated that the combinative effects of afb(1) and don (0.01 μg/ml afb(1) and 0.25 μg/ml don; 0.02 μg/ml afb(1) and 0.25 μg/ml don; 0.02 μg/ml afb(1) and 0.5 μg/ml don) were higher than that of individual mycotoxin (p < 0.05 ...201021152299
consequences of the size structure of fish populations for their effects on a generalist avian predator.size-structured interspecific interactions can shift between predation and competition, depending on ontogenetic changes in size relationships. i examined the effects of common carp (cyprinus carpio), an omnivorous fish, on the reproductive success of the red-necked grebe (podiceps grisegena), an avian gape-limited predator, along a fish size gradient created by stocking distinct age-cohorts in seminatural ponds. young-of-the-year (0+) carp were an essential food source for young grebes. only ad ...201121153742
validating and corroborating the deposition of two annual growth zones in asteriscus otoliths of common carp cyprinus carpio from south africa's largest impoundment.a total of 816 common carp cyprinus carpio asteriscus otolith pairs were collected from lake gariep, south africa. otoliths were interpreted whole, submerged in methyl salicylate and viewed under transmitted light. the precision of growth zone counts of the primary reader was estimated at 5·54 and 7·03% using the average per cent error method and the coefficient of variation, respectively. age-bias plots indicated no systematic bias between the primary reader and the three secondary readers for ...201021155779
isolation and characterization of koi herpesvirus (khv) from indonesia: identification of a new genetic lineage.koi herpesvirus (khv) is the aetiological agent of an emerging disease (khvd) associated with mass mortalities in koi and common carp and reported from at least 30 countries. we report the first isolation of khv from koi and common carp in indonesia and initial characterization of the isolates. clinical signs, histopathology and virion morphology are similar to those of isolates from other countries. phylogenetic analyses using the thymidine kinase gene amplified from each isolate and from carp ...201021158870
haematological indices are modulated in juvenile carp, cyprinus carpio l., exposed to microcystins produced by cyanobacterial water bloom.this study evaluated the influence of toxic cyanobacterial water blooms on the blood indices of the common carp, cyprinus carpio l. experimental fish were exposed to a natural population of cyanobacterial water blooms (mainly microcystis aeruginosa and m. ichthyoblabe), which contained microcystins [total concentration 133-284 μg g⁻¹ (dw), concentration in water 2.8-7.4 μg l⁻¹]. haematological indices showed marked changes in fish exposed to the cyanobacterial population in comparison with the c ...201021158871
potential use of probiotic- and triherbal extract-enriched diets to control aeromonas hydrophila infection in carp.this study reports the effect of probiotic- and triherbal extract-enriched diet on cyprinus carpio (52 +/- 2 g, n = 250) inoculated intramuscularly (50 microl) with aeromonas hydrophila (1.8 x 106 cfu ml(-1)). on day 6 post-infection, the fish were divided into 4 groups and fed with the basal diet (iu) or with diets supplemented with triherbal extract (h), sporolac (s) or lactobacillus (l). a fifth group of non-infected fish fed with the basal diet was used as a control. haematology and immunolo ...201021166313
seminoma in a koi carp cyprinus carpio: histopathological and immunohistochemical findings.a spontaneous seminoma in a 3 yr old male koi carp cyprinus carpio l. is described. the animal, presenting a symmetric abdominal enlargement, showed a celomatic multinodular, white-yellowish and firm mass that infiltrated the liver and the intestine wall. histologically, the neoplasm was non-encapsulated and poorly demarcated, showed invasive growth and was characterized by a lobular architecture, subdivided by abundant fibro-connective septa. large necrotic and calcified areas together with sma ...201021166318
concentration of polychlorinated biphenyls and organochlorine pesticides in some edible fish species from the shadegan marshes (iran).concentrations of polychlorinated biphenyls (pcbs), and organochlorine pesticides (ocps), such as dichlorodiphenyltrichloroethane (ddt) and analogs, hexachlorocyclohexanes (hchs) and hexachlorobenzene (hcb), were determined in 8 fish species collected during october and november 2007 from the largest iranian wetland, the shadegan marshes. fishes were selected on the basis of their importance in the local diet and feeding behavior. in all samples, ocps were found in higher concentrations than pcb ...201121168210
[correlation and location of est markers with cold tolerance trait of common carp (cyprinus carpio l)].expressed sequence tags (ests) are parts of complementary dnas (cdnas) with certain gene function.it provides us more information than other neutral markers. the association of est markers with phenotypes can increase our understanding of the biochemical pathways and mechanisms affecting economically important traits. in this study, 12 candidate est markers isolated from the cold-induced brain cdna of common carp (cyprinus carpio l.) were conducted the correlation analysis of marker and cold tol ...201021174346
trophic transfer of mercury and methylmercury in an aquatic ecosystem impacted by municipal sewage effluents in beijing, china.gaobeidian lake, located in beijing, china, serves as a recipient lake for effluents from a large municipal sewage treatment plant (mstp). in order to evaluate the effects of discharging mstp effluent on the mercury contamination of the local aquatic ecosystem, sediment cores, water, plankton, fish, and turtle samples were collected from gaobeidian lake for mercury speciation analysis. high concentrations of total mercury (t-hg) were detected in sediment cores (5.24-17.0 microg/g dry weight (dw) ...201021179957
[activation of the mauthner cell by direct stimulation on vagus nerve in crucian carp].to investigate the influence of the vagus nerve on the electrophysiological properties in the mauthner cell soma and the relationship between soma and viscus.200221180078
nitrosative stress during infection-induced inflammation in fish: lessons from a host-parasite infection model.the inflammatory response should be considered a protective immune reaction of the host aimed at the removal of pathogens, sometimes irrespective of negative side-effects. in this review we discuss the differential contribution of macrophages and neutrophilic granulocytes to nitrosative stress in vivo and discuss how the timing and concentration of nitric oxide (no·) are important factors determining the degree of nitrosative stress during parasite-induced inflammation. infections of common carp ...201021184662
calcitonin-like immunoreactivity in the subcommissural organ-reissner's fiber complex of some freshwater and marine teleosts.the subcommissural organ (sco) is a highly specialised circumventricular ependymal organ covering and penetrating the posterior commissure. the secretory products of the sco condense to form reissner's fiber (rf). because of its extensive secretory activity and the chemical properties of its secretion, the organ functions as similar to the neurosecretory cells. teleosts comprised of more than 20,000 extant species that show great diversity in terms of the form, habit and habitat. affinity of cal ...201121184824
genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional orf that could be expressed in fish cell.genomic sequence of scophthalmus maximus rhabdovirus (smrv) isolated from diseased turbot has been characterized. the complete genome of smrv comprises 11,492 nucleotides and encodes five typical rhabdovirus genes n, p, m, g and l. in addition, two open reading frames (orf) are predicted overlapping with p gene, one upstream of p and smaller than p (temporarily called ps), and another in p gene which may encodes a protein similar to the vesicular stomatitis virus c protein. the c orf is containe ...201021185339
study on apoptotic effects of neurotoxin anatoxin-a on fish immune cells.the aim of this study was to assess the possible in vitro apoptotic effects of anatoxin-a on the selected immune cells isolated from the blood of carp.201021187820
effects of subchronic exposure to spartakus (prochloraz) on common carp cyprinus carpio.the aim of the study was to investigate effects of the fungicide formulation spartakus (prochloraz 450 g.l-1) on common carp cyprinus carpio through biometric, biochemical, haematological and antioxidant indices, induction of xenobiotic metabolizing enzymes and histological examination of selected tissues.201021187831
enzymatic-microwave assisted extraction and high-performance liquid chromatography-mass spectrometry for the determination of selected veterinary antibiotics in fish and mussel samples.a new method based on enzymatic-microwave assisted extraction prior to high performance liquid chromatography (hplc) has been developed for the determination of 11 antibiotics (drugs) and the main metabolites of five of them in fish tissue and mussel samples. the analysed compounds were sulfadiazine (sdi), n(4)-acetylsulfadiazine (ndi), sulfamethazine (smz), n(4)-acetylsulfamethazine (nmz), sulfamerazine (smr), n(4)-acetylsulfamerazine (nmr), sulfamethoxazole (smx), trimetroprim (tmp), amoxicill ...201121190806
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